Yeast Gene Terminator Selection for Expression Control
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Solution Overview
Problem
Current methods for regulating gene expression in yeast lack effective identification of nucleotide sequences in terminator regions that contribute to high or suppressed gene expression outputs, making it difficult to optimize expression systems for desired levels.
Innovation Solution
Selecting specific yeast gene terminator regions based on expression intensity data to either enhance or suppress the output of an arbitrary gene in yeast, using a combination of high- or low-expression terminator regions with promoters to regulate gene expression levels.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If conventional promoter regions are used for gene expression in yeast, then gene expression can be achieved, but the expression output cannot be precisely regulated or optimized
Solution Approach 1:
The patent applies parameter changes by systematically varying the terminator region sequences to different expression levels. By selecting terminators with different nucleotide compositions and structures from yeast genes, the expression output can be precisely controlled across a range of levels, transforming a fixed-expression system into one with adjustable parameters.
Solution Approach 2:
The patent segments the gene expression system into distinct functional components: promoter regions and terminator regions. By independently optimizing and combining different promoter-terminator pairs, the system achieves modular control over expression levels, allowing flexible regulation without redesigning the entire expression cassette.
2Manufacturing precision
If terminator regions are selected based on expression intensity data, then precise regulation of gene expression is achieved, but the complexity of selecting and characterizing terminator regions increases
Solution Approach 1:
The patent performs preliminary characterization of terminator regions from various yeast genes, measuring their expression intensities in advance and cataloging them. This pre-established library of characterized terminators with known expression levels eliminates the need for repeated optimization experiments, reducing future complexity while maintaining precise control.
Solution Approach 2:
The patent creates a library of terminator region sequences with documented expression characteristics that can be copied and reused across different expression constructs. Once a terminator's expression level is characterized, it can be replicated in multiple applications without re-characterization, reducing overall system complexity.
Data Source
AI summary
A method for producing an expression product of an exogenous gene in a yeast, including using at least one yeast gene terminator regions selected based on expression intensity data related to the expression intensity of yeast gene terminator regions to cause expression of a gene in a yeast.