Zeaxanthin-Enriched Egg Yolk via Paracoccus Bacterial Feed

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Solution Overview

Problem

Current methods for producing zeaxanthin-enriched eggs require high amounts of zeaxanthin sources, leading to inefficient human blood concentration increases and stability issues due to low zeaxanthin concentrations in egg yolks, particularly when using spirulina or Chinese wolfberry fruit as feed additives.

Innovation Solution

Incorporating zeaxanthin-producing bacteria from the genus Paracoccus into poultry feedstuff, such as Paracoccus carotinifaciens, to enhance zeaxanthin levels in egg yolks, allowing for higher concentrations with lower additive amounts and stable supply.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Quantity of substance

If high amounts of zeaxanthin sources (spirulina, Chinese wolfberry fruit) are added to feedstuffs, then zeaxanthin levels in egg yolks increase, but the efficiency of human blood concentration increase remains low and stability issues occur

Engineering Contradiction:
Improvezeaxanthin concentration in egg yolkVSAvoidefficiency of human blood zeaxanthin level increase
Core Design Contradiction:
Quantity of substanceVSProductivity

Solution Approach 1:

The patent changes the source parameter from plant-based zeaxanthin (spirulina, wolfberry) to bacterial-based zeaxanthin (Paracoccus carotinifaciens), which has higher bioavailability and efficiency for human blood concentration increase. This parameter change resolves the contradiction by maintaining high egg yolk zeaxanthin levels while improving the efficiency of human blood zeaxanthin increase.

Inventive Principle:
Principle #35Parameter changes

Solution Approach 2:

The patent uses bacterial culture (Paracoccus carotinifaciens) as a temporary, easily degradable feed additive that can be efficiently metabolized by chickens. The bacteria serve as a short-living, highly effective zeaxanthin source that is converted into stable egg yolk zeaxanthin, resolving the stability issue while maintaining high efficiency.

Inventive Principle:
Principle #27Cheap short-living objects (Disposable)

2Quantity of substance

If plant-based zeaxanthin sources (spinach, corn, oranges) are used, then dietary zeaxanthin intake is provided, but the concentration cannot be easily increased in an artificial manner

Engineering Contradiction:
Improvezeaxanthin concentrationVSAvoidartificial concentration increase capability
Core Design Contradiction:
Quantity of substanceVSEase of manufacture

Solution Approach 1:

The patent changes the zeaxanthin source from plant-based to bacterial-based (Paracoccus carotinifaciens), which can be cultured and concentrated artificially through controlled fermentation processes. This enables easy artificial concentration increase while maintaining natural zeaxanthin properties, resolving the contradiction between concentration and ease of manufacture.

Inventive Principle:
Principle #35Parameter changes

Solution Approach 2:

The bacterial culture system self-produces zeaxanthin through metabolic processes when provided with appropriate nutrients. The Paracoccus carotinifaciens bacteria automatically synthesize and accumulate zeaxanthin in culture medium, enabling easy artificial concentration without complex extraction processes from plants.

Inventive Principle:
Principle #25Self-service

3Quantity of substance

If supplements containing zeaxanthin are used to increase human blood zeaxanthin levels, then blood concentration can be increased, but the proportion of increase to intake is low (0.01-0.06 μM/(mg/Day))

Engineering Contradiction:
Improvehuman blood zeaxanthin concentrationVSAvoidproportion of increase to intake efficiency
Core Design Contradiction:
Quantity of substanceVSProductivity

Solution Approach 1:

The patent changes the zeaxanthin delivery system from direct supplementation to indirect delivery through egg yolk. The egg yolk matrix enhances bioavailability and absorption efficiency, resulting in a higher proportion of increase to intake (0.28 μM/(mg/Day)) compared to direct supplements, resolving the productivity contradiction.

Inventive Principle:
Principle #35Parameter changes

Solution Approach 2:

The patent uses chicken egg yolk as an intermediary carrier for zeaxanthin delivery to humans. The egg yolk matrix protects zeaxanthin during digestion and enhances its absorption in the human intestinal tract, significantly improving the efficiency of blood concentration increase compared to direct supplement intake.

Inventive Principle:
Principle #24Intermediary (Mediator)

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

This method effectively increases zeaxanthin concentrations in egg yolks to 3.5 mg to 10 mg per 100 g, leading to higher human blood zeaxanthin levels with improved efficiency and stability compared to existing methods.

Implementation Method 1

a method that involves administering to a chicken, zeaxanthin-producing bacteria; and collecting an egg from the chicken

Methodology Applied
Scientific EffectBacterial production: Fermentation

Data Source

PatentUS9192588B2Method for producing a zeaxanthin-enriched chicken egg
Publication Date: 2015.11.24 ENEOS MATERIALS CORP
  • US9192588B2 patent drawing
  • US9192588B2 patent drawing

AI summary

An object of the present invention is to provide zeaxanthin-enriched poultry eggs containing zeaxanthin at high concentrations. The present invention specifically relates to zeaxanthin-enriched poultry eggs obtained by feeding poultry with a poultry feedstuff containing zeaxanthin-producing bacteria.