Truncated ZM-ODP2 Polypeptide for Maize Haploid Induction
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Solution Overview
Problem
Current methods for producing doubled haploid plants in maize are inefficient, as many maternal haploid embryos fail to regenerate into fertile plants, and require extensive pollination control and prolonged time for self-fertilized lines to reach isogenic states.
Innovation Solution
A method involving the use of a polynucleotide sequence encoding a truncated ZM-ODP2 polypeptide to promote chromosome doubling in diploid embryos, allowing for the regeneration of doubled haploid plants without chemical chromosome doubling agents, and utilizing genetic components like cyclin gene family members and genome-editing tools for enhanced haploid induction and chromosome doubling.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If maternal haploid embryos are selected following fertilization with haploid inducer pollen, then haploid embryos are produced, but most fail to regenerate into fertile doubled haploid plants
Solution Approach 1:
The patent applies preliminary action by introducing the truncated ZM-ODP2 polypeptide into diploid embryos before they undergo development. This pre-treatment promotes chromosome doubling during early embryogenesis, ensuring that when these embryos are later regenerated, they already contain doubled chromosomes and can develop into fertile doubled haploid plants without requiring subsequent chemical treatment or having low regeneration success rates.
2Productivity
If chemical chromosome doubling agents are used to produce doubled haploid plants, then chromosome doubling is achieved, but the process requires extensive pollination control and prolonged time
Solution Approach 1:
The patent applies self-service by engineering the plant system to perform chromosome doubling autonomously through the expression of the truncated ZM-ODP2 polypeptide. The modified diploid embryos self-duplicate their chromosomes during natural development without requiring external chemical agents or complex pollination control protocols. This self-driven chromosome doubling simplifies the breeding process and accelerates productivity.
Solution Approach 2:
The patent applies parameter changes by modifying the genetic composition of the embryo through introduction of the truncated ZM-ODP2 polypeptide. This genetic modification changes the developmental parameters of the embryo, causing it to naturally undergo chromosome doubling during its normal development process, thereby eliminating the need for external chemical treatments and reducing pollination control complexity.
3Stability of the object's composition
If self-fertilized lines are propagated to reach isogenic states, then genetic uniformity is achieved, but extensive time is required
Solution Approach 1:
The patent applies preliminary action by achieving chromosome doubling during the embryo stage through truncated ZM-ODP2 polypeptide expression. This creates genetically uniform doubled haploid plants in a single generation, eliminating the need for multiple generations of self-fertilization that would otherwise be required to achieve isogenic states. The genetic uniformity is established upfront rather than through prolonged propagation.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This method improves the efficiency of producing fertile doubled haploid plants by promoting chromosome doubling and reducing the need for chemical agents, thereby accelerating the breeding process and enhancing hybrid performance in maize.
Implementation Method 1
selecting a haploid embryo expressing the polynucleotide sequence encoding the truncated ZM-ODP2 polypeptide and lacking the genome of the haploid inducer plant, wherein the truncated ZM-ODP2 polypeptide promotes chromosome doubling of the haploid embryo to produce a doubled haploid embryo
Implementation Method 2
providing a diploid embryo of a plant with a polynucleotide sequence encoding at least a truncated ZM-ODP2 polypeptide; regenerating a T0 plant from the diploid embryo, wherein the T0 plant expresses the polynucleotide sequence encoding the truncated ZM-ODP2 polypeptide
Data Source
AI summary
The present disclosure provides methods of generating doubled haploid plants using a polynucleotide sequence encoding the truncated ZM-ODP2 polypeptide for parthenogenic haploid induction and haploid chromosome doubling.