ZmPLD3 Gene Knockout for Maize Haploid Breeding Efficiency
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Solution Overview
Problem
Current maize haploid breeding methods are time-consuming and labor-intensive, and there is a need to identify genes that regulate haploid induction to increase the induction rate and efficiency of maize hybrid breeding.
Innovation Solution
The use of the ZmPLD3 gene is suppressed or knocked out using CRISPR/Cas9 gene editing technology to produce transgenic plants, which are then used as male parents to produce maternal haploids, facilitating the selection and breeding of new haploid induction lines with high induction rates.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If conventional haploid breeding methods are used, then pure lines can be obtained, but the breeding process is time-consuming and labor-intensive
Solution Approach 1:
The invention changes the genetic parameter by introducing a mutation in the ZmPLD3 gene of the male parent, which fundamentally alters the haploid induction mechanism. This genetic parameter change enables rapid haploid production without following the time-consuming conventional breeding steps, directly resolving the contradiction between breeding efficiency and time consumption
Solution Approach 2:
The invention performs preliminary action by pre-establishing a male parent line with a mutated ZmPLD3 gene that has high haploid induction capability. This pre-prepared induction line can be directly used for crossing with various female parents, eliminating the need for time-consuming conventional breeding procedures and enabling rapid pure line production
2Productivity
If conventional haploid induction lines are used, then some haploids can be obtained, but the induction rate needs to be increased
Solution Approach 1:
The invention changes the genetic parameter by introducing a specific mutation in the ZmPLD3 gene, which fundamentally alters the haploid induction mechanism. This genetic modification creates a new induction line with significantly enhanced induction rate and reliability, resolving the contradiction between induction rate and performance consistency
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This method significantly shortens the breeding process by achieving high haploid induction rates, providing a new approach for improving maize breeding efficiency and understanding the genetic mechanisms of haploid induction.
Implementation Method 1
Above-mentioned mutation is achieved by CRISPR/Cas9 gene editing technology; the target sequence of the CRISPR/Cas9 is as shown in SEQ ID NO: 2 of the sequence listing
Implementation Method 2
The sgRNA sequence of above-mentioned CRISPR/Cas9 is as shown in SEQ ID NO: 3 of the sequence listing. DNA molecule that hybridizes to DNA molecules defined in (1) under stringent conditions and encode proteins with the same function
Data Source
AI summary
A gene ZmPLD3 for inducing maize maternal haploid production and its application thereof. Transgenic homozygous mutant plants or their progeny can be obtained by knocking out the ZmPLD3 gene in maize, and maize maternal haploids can be produced by hybridizing them as paternal materials with other maize materials. A series of allelic mutations of the gene having maternal haploid induction function through hybridization were obtained. The experiments showed that the mutation of maize phospholipase PLD3 could lead to the production of maize maternal haploid, which provides new thoughts for revealing the biological role of phospholipase in maize maternal haploid induction process. At the same time, the mutant individuals have the maternal haploid induction ability in maize, which is of great significance for breeding new types of haploid induction lines with high haploid induction rate and improving the efficiency of maize haploid breeding.