Brain stereotaxic combined with continuous gas anesthesia drug delivery system and brain stereotaxic anesthesia depth quantitative regulation method

Abstract

The present application belongs to the field of biological experimental technology, and specifically relates to a brain stereotaxic combined with continuous gas anesthesia drug delivery system and a brain stereotaxic anesthesia depth quantitative adjustment method. A brain stereotaxic combined with continuous gas anesthesia drug delivery system, characterized in that it includes a first ear bar, a second ear bar, an anesthesia headgear and a gas delivery device; the anesthesia headgear is used to deliver anesthetic gas and / or oxygen to the experimental model and assist the first ear bar and the second ear bar to complete the fixation of the experimental model. The present application improves the structure and function of the original brain stereotaxic instrument, making it an animal brain stereotaxic instrument that can fix rats and give continuous gas anesthesia, avoiding experimental errors and accidental deaths caused by too shallow or too deep anesthesia depth of animals during the experiment, and has technical effects such as more stable model state, higher experimental accuracy, and experimental results closer to the actual situation.

Description

Technical Field

[0001] The present application belongs to the field of biological experiment technology, and specifically relates to a brain stereotaxic combined with continuous gas anesthesia drug delivery system and a method for quantitatively regulating the depth of brain stereotaxic anesthesia. Background Art

[0002] Stereotaxic apparatus, also known as brain fixation device, uses a three-dimensional coordinate system defined by the marks on the outside of the skull or other reference points to determine the position of certain subcortical neural structures, so as to conduct targeted stimulation, destruction, drug injection, potential guidance and other studies under non-direct exposure. Animal brain stereotaxic apparatus is an important research equipment in the fields of neuroanatomy, neurophysiology, neuropharmacology and neurosurgery, and can be used to establish animal models of Parkinson's disease, epilepsy, brain tumor models, learning and memory, brain neural stem cell transplantation, cerebral ischemia and other studies.

[0003] During the animal modeling process, we often fix the animals on a stereotaxic apparatus after intravenous or intraperitoneal injection of anesthetic drugs. Since some modeling takes a long time, the animals are prone to wake up during the modeling process, which affects the modeling effect. In addition, the dose of intravenous or intraperitoneal anesthetic drugs is difficult to control, which can easily cause death due to overdose of anesthesia or insufficient depth of anesthesia, greatly affecting the progress of the experiment. Summary of the invention

[0004] In view of this, the present application provides a brain stereotaxic combined with continuous gas anesthesia drug delivery system and a brain stereotaxic anesthesia depth quantitative adjustment method to solve the technical problems existing in the prior art.

[0005] The brain stereotactic combined with continuous gas anesthesia drug delivery system provided by the present application to solve the technical problem is:

[0006] A brain stereotactic combined with continuous gas anesthesia drug delivery system, characterized in that it comprises a first ear bar, a second ear bar, an anesthesia headgear and a gas delivery device; the first ear bar and the second ear bar are used to fix an experimental model; the anesthesia headgear is used to deliver anesthetic gas and / or oxygen to the experimental model and assist the first ear bar and the second ear bar to complete the fixation of the experimental model; the gas delivery device comprises an oxygen supply tube and an air supply tube, the two ends of the oxygen supply tube are respectively connected to the anesthesia headgear and the oxygen source, and the two ends of the air supply tube are respectively connected to the anesthesia headgear and the anesthetic gas source.

[0007] As a preferred embodiment of the brain stereotaxic combined with continuous pneumatic anesthesia drug delivery system, the brain stereotaxic combined with continuous pneumatic anesthesia drug delivery system also includes a model adapter, and the anesthesia headgear is arranged on the model adapter, which can adapt to different experimental models under the action of the model adapter.

[0008] As a preferred embodiment of the brain stereotaxic combined with continuous gas anesthesia drug delivery system, the brain stereotaxic combined with continuous gas anesthesia drug delivery system also includes a control component; the gas delivery device also includes an oxygen supply valve and a gas supply valve; the oxygen supply valve and the gas supply valve are electrically connected to the control component; the control component can adjust the oxygen supply amount and / or gas supply amount by controlling the oxygen supply valve and the gas supply valve.

[0009] As a preferred embodiment of the brain stereotaxic combined with continuous pneumatic anesthesia drug delivery system, the brain stereotaxic combined with continuous pneumatic anesthesia drug delivery system also includes a respiratory rate sensor, which is arranged in the anesthesia headgear and is used to measure the respiratory intensity of the experimental model.

[0010] As a preferred embodiment of the brain stereotaxic combined with continuous pneumatic anesthesia drug delivery system, the brain stereotaxic combined with continuous pneumatic anesthesia drug delivery system also includes a temperature probe and a blood pressure probe, which are respectively arranged in the first ear bar and the second ear bar and electrically connected to the control component, and are respectively used to test the body temperature and blood pressure of the experimental model; the control component can collect the vital sign parameters of the experimental model in real time, evaluate the anesthesia depth of the experimental model according to the collected vital sign parameters, and when the anesthesia depth does not meet the experimental requirements, the oxygen supply valve and / or the gas supply valve are used to adjust the oxygen supply and / or gas supply amount.

[0011] As a preferred embodiment of the brain stereotaxic combined with continuous pneumatic anesthesia drug delivery system, the brain stereotaxic combined with continuous pneumatic anesthesia drug delivery system further comprises an infrared receiver, and the infrared receiver is used to receive infrared rays radiated by the experimental model.

[0012] As a preferred embodiment of the brain stereotaxic combined with continuous gas anesthesia drug delivery system, the brain stereotaxic combined with continuous gas anesthesia drug delivery system further comprises at least one flow meter, and the flow meter is used for quantitatively adjusting the supply amount of anesthetic gas and / or oxygen.

[0013] The method for quantitatively adjusting the depth of stereotaxic anesthesia provided by the present application to solve the technical problem is as follows:

[0014] A method for quantitatively regulating the depth of brain stereotaxic anesthesia is characterized in that it includes the following steps: a vital sign parameter collection step, collecting the vital sign parameters of an experimental model; an anesthesia depth evaluation step, evaluating whether the current anesthesia depth of the experimental model reaches an ideal state through the collected vital sign parameters; an anesthesia depth adjustment step, if the current anesthesia depth does not reach the ideal state, adjusting the anesthesia depth of the experimental model by adjusting the supply of anesthetic gas until the anesthesia depth reaches the ideal state; if the current anesthesia depth reaches the ideal state, maintaining the current anesthesia depth.

[0015] As a preferred method for quantitatively adjusting the depth of anesthesia, the vital sign parameters collected in the vital sign parameter collection step include body temperature, blood pressure and respiratory intensity; in the anesthesia depth assessment step, the real-time body temperature, real-time blood pressure and real-time respiratory intensity of the experimental model are judged respectively to determine whether the real-time body temperature, real-time blood pressure and real-time respiratory intensity are all within the range of the ideal anesthesia depth.

[0016] As a preferred method for quantitatively adjusting the depth of anesthesia, the vital sign parameters collected in the vital sign parameter collection step also include the real-time infrared intensity radiated by the experimental model; the anesthesia depth assessment step also includes determining whether the real-time infrared intensity is within the range of the ideal anesthesia depth.

[0017] As a preferred method for quantitatively adjusting the depth of anesthesia, in the anesthesia depth assessment step, when judging whether the real-time body temperature, real-time blood pressure and real-time respiratory intensity of the experimental model are within the range of the ideal anesthesia depth, a finite parameter maximum tension cohesive body model is used as a reference model. When the real-time parameters are within the numerical range constrained by the finite parameter maximum tension cohesive body model, it is considered to be within the range of the ideal anesthesia depth.

[0018] Beneficial technical effects:

[0019] 1. The present application improves the equipment on the basis of the brain stereotaxic instrument by replacing the nose clip with an anesthesia headgear, which not only retains the function of fixing rats, but also can be connected to the gas anesthesia equipment, so that the gas anesthesia administration can be adjusted at any time during the modeling process, the anesthesia quality can be improved, and the continuous anesthesia state of the animals can be ensured, which is conducive to the smooth progress of the experiment and reduces experimental accidents caused by excessive or insufficient anesthetic dosage.

[0020] 2. The advantages of continuous inhalation gas anesthesia over traditional intravenous anesthesia or intraperitoneal anesthesia are: ① Inhalation anesthetics are metabolized and decomposed in the body, and most of them are excreted from the lungs in their original form, so inhalation anesthesia has higher safety and effectiveness. ② Inhalation anesthetics have the advantages of fast onset and fast awakening. ③ Inhalation gas anesthesia can adjust the depth of anesthesia at any time by adjusting the gas flow rate during the experiment, which is simple, convenient and fast.

[0021] 3. This application adopts a finite parameter maximum tension condensate model as a reference model for evaluating the depth of anesthesia. Compared with various existing anesthesia depth assessment methods, it has the technical effects of a more stable model, higher experimental accuracy, evaluation results closer to the actual situation, and better consistency of experimental results.

[0022] The technical solution and technical effects of the present application are described in detail below in conjunction with the accompanying drawings and specific implementation methods. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 : Schematic diagram of the structure of the brain stereotaxic combined with continuous gas anesthesia drug delivery system;

[0024] Figure 2 :Flow chart of quantitative regulation method of brain stereotaxic anesthesia depth;

[0025] Figure 3 : Schematic diagram of the principle of the finite parameter maximum tension condensate reference model;

[0026] Logo Description:

[0027] 10-first ear bar, 20-second ear bar, 30-anesthesia headgear, 40-gas delivery device, 50-model adapter, 60-control component, 70-respiration rate sensor, 80-temperature probe, 90-blood pressure probe, 100-infrared receiver;

[0028] 410- oxygen supply pipe, 420- gas supply pipe, 430- oxygen supply valve, 440- gas supply valve. DETAILED DESCRIPTION

[0029] First preferred embodiment

[0030] See also Figure 1-Figure 2 The brain stereotaxic combined continuous gas anesthesia drug delivery system provided in the present application includes a first ear bar 10, a second ear bar 20, an anesthesia headgear 30 and a gas delivery device 40, a model adapter 50, a control component 60, a respiratory rate sensor 70, a temperature probe 80 and a blood pressure probe 90.

[0031] The first ear bar 10 and the second ear bar 20 are used to fix the experimental model (a rat model in this embodiment).

[0032] The anesthesia headgear 30 is used to deliver anesthesia gas and / or oxygen to the experimental model and assist the first ear bar 10 and the second ear bar 20 in fixing the experimental model.

[0033] The gas delivery device 40 includes an oxygen delivery tube 410, an air delivery tube 420, an oxygen delivery valve 430, and an air delivery valve 440. The two ends of the oxygen delivery tube 410 are respectively connected to the anesthesia headgear 30 and an oxygen source such as an oxygen cylinder, and the two ends of the air delivery tube 420 are respectively connected to the anesthesia headgear 30 and an anesthetic gas source such as an anesthetic gas cylinder. The oxygen delivery valve 430 and the air delivery valve 440 are electrically connected to the control component 60; the control component 60 can adjust the oxygen delivery amount and / or the air delivery amount by controlling the oxygen delivery valve 430 and the air delivery valve 440.

[0034] The model adapter 50 , on which the anesthesia headgear 30 is disposed, can adapt to different experimental models under the action of the model adapter 50 .

[0035] The respiratory rate sensor 70 is arranged in the anesthesia headgear 30, and is used to measure the real-time respiratory intensity of the experimental model. The temperature probe 80 and the blood pressure probe 90 are respectively arranged in the first ear bar 10 and the second ear bar 20 and are electrically connected to the control component 60, and are respectively used to test the real-time body temperature and real-time blood pressure of the experimental model. The control component 60 can collect the vital sign parameters of the experimental model in real time, and evaluate the anesthesia depth of the experimental model according to the collected vital sign parameters. When the anesthesia depth does not meet the experimental requirements, the oxygen supply valve 430 and / or the gas supply valve 440 are used to adjust the oxygen supply and / or gas supply.

[0036] Principle description: Fix the experimental model on the experimental table with the first ear bar, the second ear bar and the anesthesia headgear, and complete the stereoscopic positioning of the experimental model with the help of various brain stereotaxic instruments in the prior art, and then carry out the biological model experiment according to the experimental plan. When the anesthesia depth of the experimental model does not reach the ideal state, such as when the anesthesia is too light or too deep, the experimenter can make appropriate adjustments to the anesthesia depth according to the real-time state of the model by observing the flow meter, such as increasing the amount of anesthetic gas or increasing the oxygen supply, etc. The automated real-time evaluation and quantitative adjustment of the anesthesia state of the experimental model can also be achieved through the regulation of the control components.

[0037] The method for quantitatively adjusting the depth of stereotaxic anesthesia provided by the present application comprises the following steps:

[0038] The step of collecting physical sign parameters is to collect physical sign parameters of the experimental model;

[0039] Anesthesia depth assessment step, assessing whether the current anesthesia depth of the experimental model has reached the ideal state through the collected vital sign parameters;

[0040] The anesthesia depth adjustment step is as follows: if the current anesthesia depth has not reached the ideal state, the anesthesia depth of the experimental model is adjusted by adjusting the supply of anesthetic gas until the anesthesia depth reaches the ideal state; if the current anesthesia depth reaches the ideal state, the current anesthesia depth is maintained.

[0041] in:

[0042] The physical sign parameters collected in the physical sign parameter collection step include body temperature t, blood pressure p and respiratory intensity bs;

[0043] In the anesthesia depth assessment step, the real-time body temperature t, real-time blood pressure p and real-time respiratory intensity bs of the experimental model are judged respectively to determine whether the real-time body temperature t, real-time blood pressure p and real-time respiratory intensity bs are all within the range of the ideal anesthesia depth. When judging whether the real-time body temperature t, real-time blood pressure p and real-time respiratory intensity bs of the experimental model are within the range of the ideal anesthesia depth, the finite parameter maximum tension cohesive body model is used as the reference model. When the real-time parameters are within the numerical range of the finite parameter maximum tension cohesive body constraints, it is considered to be within the range of the ideal anesthesia depth.

[0044] See also Figure 3 When there is only one physical parameter that determines the depth of anesthesia, such as body temperature t, the ideal real-time body temperature t of the experimental model is between the range t 1 -t 2 Between; Temperature range t 1 -t 2 A flat plate-shaped model body is defined in space, which is infinitely extended in the positive and negative directions of the blood pressure p axis and the respiratory intensity bs axis; when there are two physical parameters that limit the depth of anesthesia, such as real-time body temperature t and real-time blood pressure p, the temperature range t 1 -t 2 and blood pressure range p 1 -p 2 A square model body is defined in space, which is infinitely extended in the positive and negative directions of the respiratory intensity bs axis; when there are three physical parameters that define the depth of anesthesia, such as real-time body temperature t, real-time blood pressure p and real-time respiratory intensity bs, the temperature range t 1 -t 2 , blood pressure range p 1 -p 2 and respiratory intensity bs 1 -bs 2 A rectangular model body is defined in space, and all model states that meet the requirements of the ideal state are within the rectangular model body.

[0045] It can be seen from the spatial contraction caused by parameter expansion that each additional model state evaluation parameter limits the expansion of the model body in one direction, adds a pair of mutually parallel parameter planes that limit the model body, and the obtained model body is becoming more and more effective. However, the parameters used to evaluate the anesthesia state of the experimental model may be infinite, such as at least experimental model brain waves, stress potentials, infrared radiation intensity, etc. Each evaluation parameter that can be considered is added to the cubic model body (that is, the three-parameter system model body), and pairs of mutually parallel parameter planes used to limit the model body will be formed at the edges of the model body. When the number of parameter planes is sufficient (that is, when the anesthesia state evaluation parameters considered are sufficient), a model body with infinite edges or a model body with a surface close to a smooth surface will be formed, that is, the model body named by the finite parameter maximum tension condensate in this application. When the value range of the parameters used to limit the depth of anesthesia is uniform and equal, the finite parameter maximum tension condensate further condenses into a sphere. When the real-time parameter is within the numerical range constrained by the finite parameter maximum tension condensate, it is considered to be within the range of the ideal anesthesia depth. The use of the spherical model as a reference model has undoubtedly taken into account all the evaluation parameters that can evaluate the depth of anesthesia. However, although the ideal state defined by the sphere can achieve the theoretical ideal state, it may not be suitable for all experimental scenarios due to overly harsh conditions.

[0046] The advantage of using a finite parameter maximum tension cohesive body model to replace various existing anesthesia depth assessment methods is that although some edges are abandoned to meet the requirements of the ideal state of finite parameters, the experimental model is not actually in the anesthesia state of the optimized experimental state, which is conducive to improving the health of the experimental model, improving the experimental accuracy, and making the experimental results closer to the actual situation. Those skilled in the art can use various existing technologies to obtain a finite parameter maximum tension cohesive body, and the following is one of the feasible solutions:

[0047] Select the three most informative parameters for assessing the depth of anesthesia, such as body temperature t, blood pressure p, and respiratory intensity bs;

[0048] Determine the ideal range of anesthesia depth assessment parameters, that is, the temperature range t 1 -t 2 , blood pressure range p 1 -p 2 and respiratory intensity bs 1 -bs 2 ;

[0049] Draw a cubic model body based on the ideal value range of the evaluation parameters;

[0050] Fillet each edge of the cubic model body with the maximum curvature and ensure that the fillet curvatures on the corresponding edges are equal and that each edge has a fillet, forming a finite parameter maximum tension condensate;

[0051] The boundary curve or straight line of the finite parameter maximum tension cohesion is determined as a basis for judging whether the real-time vital sign parameters are within the value range of the ideal anesthesia state defined by the finite parameter maximum tension cohesion.

[0052] Those skilled in the art can use 3D drawing tools such as PROE and analysis tools such as Matlab to obtain the finite parameter maximum tension cohesive body and its defined boundary curve or straight line, which will not be elaborated here.

[0053] This application uses a finite parameter maximum tension condensate model as a reference model for evaluating the depth of anesthesia. Compared with various existing anesthesia depth assessment measures, it has technical effects such as assessment results that are closer to the actual situation, a more stable model, higher experimental accuracy, and better consistency of experimental results.

[0054] Second preferred embodiment

[0055] In another preferred embodiment of the present application, the same hardware results and sleep depth assessment method as those in the first preferred embodiment are used, except that the brain stereotactic combined with continuous gas anesthesia drug delivery system disclosed in this embodiment also includes an infrared receiver 100, which is electrically connected to the control component 60 and is used to receive infrared rays radiated by the experimental model to determine whether the real-time infrared intensity of the experimental model is within the range of ideal anesthesia depth by the intensity of the infrared rays.

[0056] The technical scheme and technical effects of the present application are described in detail above in combination with the drawings and specific embodiments of the specification. It should be noted that the specific implementation methods disclosed in the specification are only preferred embodiments of the present application, and technical personnel in the field can also develop other embodiments on this basis; any simple deformation and equivalent substitution that does not deviate from the innovative concept of the present application are covered by the present application and fall within the scope of protection of this patent.

Claims

1. A brain stereotactic combined with continuous gas anesthesia drug delivery system, characterized in that: It comprises a first ear bar (10), a second ear bar (20), an anesthesia headgear (30) and a gas delivery device (40); The first ear bar (10) and the second ear bar (20) are used to fix the experimental model; The anesthesia headgear (30) is used to deliver anesthesia gas and / or oxygen to the experimental model and assist the first ear bar (10) and the second ear bar (20) in fixing the experimental model; The gas delivery device (40) comprises an oxygen delivery tube (410) and an air delivery tube (420), wherein two ends of the oxygen delivery tube (410) are respectively connected to the anesthesia headgear (30) and an oxygen source, and two ends of the air delivery tube (420) are respectively connected to the anesthesia headgear (30) and an anesthetic gas source; The brain stereotaxic combined with continuous gas anesthesia drug delivery system also includes a control component (60); The gas delivery device (40) further comprises an oxygen delivery valve (430) and a gas delivery valve (440); The oxygen supply valve (430) and the gas supply valve (440) are electrically connected to the control component (60); The control component (60) can adjust the oxygen supply amount and / or gas supply amount by controlling the oxygen supply valve (430) and the gas supply valve (440); The brain stereotaxic combined with continuous gas anesthesia drug delivery system further comprises a respiratory rate sensor (70), wherein the respiratory rate sensor (70) is arranged in the anesthesia headgear (30) and is used to measure the respiratory intensity of the experimental model; The brain stereotaxic combined with continuous gas anesthesia drug delivery system further comprises a temperature probe (80) and a blood pressure probe (90), wherein the temperature probe (80) and the blood pressure probe (90) are respectively arranged in the first ear bar (10) and the second ear bar (20) and are electrically connected to the control component (60), and are respectively used to test the body temperature and blood pressure of the experimental model; The control component (60) can collect the vital sign parameters of the experimental model in real time, evaluate the anesthesia depth of the experimental model according to the collected vital sign parameters, and adjust the oxygen supply volume and / or gas supply volume through the oxygen supply valve (430) and / or the gas supply valve (440) when the anesthesia depth does not meet the experimental requirements; The brain stereotaxic combined with continuous gas anesthesia drug delivery system further comprises a model adapter (50), and the anesthesia headgear (30) is arranged on the model adapter (50) and can adapt to different experimental models under the action of the model adapter (50).

2. The brain stereotactic combined with continuous gas anesthesia drug delivery system according to claim 1, characterized in that: The brain stereotaxic combined with continuous gas anesthesia drug delivery system further comprises an infrared receiver (100), wherein the infrared receiver (100) is used to receive infrared rays radiated by the experimental model.

3. The brain stereotactic combined with continuous gas anesthesia drug delivery system according to claim 1, characterized in that: The brain stereotaxic combined with continuous gas anesthesia drug delivery system further comprises at least one flow meter, and the flow meter is used for quantitatively adjusting the supply amount of anesthetic gas and / or oxygen.

4. A method for quantitatively adjusting the depth of brain stereotaxic anesthesia, characterized in that: Applicable to the brain stereotactic combined with continuous gas anesthesia drug delivery system as described in claim 1; The steps include: The step of collecting physical sign parameters is to collect physical sign parameters of the experimental model; Anesthesia depth assessment step, assessing whether the current anesthesia depth of the experimental model has reached the ideal state through the collected vital sign parameters; anesthesia depth adjustment step, if the current anesthesia depth does not reach the ideal state, the anesthesia depth of the experimental model is adjusted by adjusting the supply of anesthetic gas until the anesthesia depth reaches the ideal state; if the current anesthesia depth reaches the ideal state, the current anesthesia depth is maintained; The physical sign parameters collected in the physical sign parameter collection step include body temperature, blood pressure and respiratory intensity; In the anesthesia depth assessment step, the real-time body temperature, real-time blood pressure and real-time respiratory intensity of the experimental model are judged respectively to determine whether the real-time body temperature, real-time blood pressure and real-time respiratory intensity are all within the range of the ideal anesthesia depth.

5. The method for quantitatively adjusting the depth of stereotaxic anesthesia according to claim 4, characterized in that: The physical sign parameters collected in the physical sign parameter collection step also include the real-time infrared intensity radiated by the experimental model; The anesthesia depth assessment step also includes determining whether the real-time infrared intensity is within the range of an ideal anesthesia depth.

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