Treponema pallidum recombinant antigen and method for preparing the same
By preparing recombinant antigens fused with four basic protein fragments of Treponema pallidum, the sensitivity and specificity issues in Treponema pallidum antibody detection were resolved, achieving high sensitivity and high specificity in Treponema pallidum antibody detection.
CN115856298BActive Publication Date: 2025-11-18SUZHOU CENTURY COORDINATE BIOTECHNOLOGY CO LTD +2
2 Cites 0 Cited by
Patent Information
- Application Number
- CN202211283535.5
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Filing Date
- 2022-10-20
- Publication Date
- 2025-11-18
- Estimated Expiration
- 2042-10-20
AI Technical Summary
Technical Problem
Existing methods for detecting Treponema pallidum antibodies have low sensitivity and specificity, making it difficult to accurately diagnose syphilis infection.
Method used
A recombinant antigen fused with four basic protein fragments of Treponema pallidum was used to prepare the Treponema pallidum recombinant antigen through gene cloning technology. The target protein was then purified by IPTG-induced expression to construct a Treponema pallidum-specific multisite recombinant protein for syphilis antibody detection.
Benefits of technology
It improved the sensitivity and specificity of Treponema pallidum antibody detection, achieving a detection rate of 100% and a specificity of 98%, while reducing the false detection rate.
✦ Generated by Eureka AI based on patent content.
Abstract
The application discloses a Treponema pallidum recombinant antigen and a preparation method thereof, and belongs to the technical field of Treponema pallidum antibody detection. In order to solve the technical problems of low sensitivity and low specificity in syphilis antibody detection, the application provides a Treponema pallidum recombinant antigen, the amino acid sequence of the recombinant antigen is shown as SEQ ID NO. 1, and a Treponema pallidum antibody detection kit, the kit comprising the Treponema pallidum recombinant antigen. The Treponema pallidum recombinant protein antigen of the application can specifically recognize the antibody of the serum species of Treponema pallidum, and due to the four different protein fragments, the sensitivity and specificity are greatly improved, and the Treponema pallidum recombinant protein antigen is the best candidate antigen of the Treponema pallidum antibody.
Need to check novelty before this filing date? Find Prior Art