An engineered halomonas sp. and a method for producing acetoin thereof
By engineering *Haloxylonus* to express acetolactate synthase and decarboxylase, and combining this with metabolic engineering strategies, the high energy consumption and safety issues in existing acetoin production technologies have been resolved, achieving low-cost and high-efficiency acetoin production.
Patent Information
- Application Number
- CN202410803451.2
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Filing Date
- 2024-06-20
- Publication Date
- 2026-08-25
- Estimated Expiration
- 2044-06-20
AI Technical Summary
Existing chemical synthesis methods for producing acetoin are energy-intensive, costly, and cause serious environmental pollution, while high-yield strains of biosynthesis are mostly pathogenic bacteria, which limits large-scale industrial application.
By engineering *Halomonas* to express or overexpress acetolactate synthase and acetolactate decarboxylase, knocking out or downexpressing PHA hydratase and acetyl-CoA, and combining various metabolic engineering strategies such as downregulating acetoin degradation pathways, enhancing membrane permeability and precursor supply, and adjusting enzyme expression levels, the production efficiency of acetoin can be improved.
The stable production of acetoin using glucose as a substrate under low-salt and low-oxygen conditions has reduced production costs, improved production efficiency, and enabled large-scale, safe, and reliable acetoin production.