73 results about "Halomonas salina" patented technology

The invention provides a method for producing L-phenylalanine, 4-hydroxymandelic acid and / or a PHA material through a one-carbon resource and application of the L-phenylalanine, the 4-hydroxymandelic acid and / or the PHA material. According to the method, a halomonas strain which cannot be directly produced by using a one-carbon resource originally is modified, and a carbon dioxide fixed gene is added, so that recombinant bacteria capable of producing L-phenylalanine and 4-hydroxymandelic acid are constructed, and co-production of extracellular L-phenylalanine, 4-hydroxymandelic acid and other high-added-value compounds and intracellular polyhydroxyalkanoate (PHA) is realized. According to the invention, a more economic, sustainable and high-benefit platform for producing L-phenylalanine, 4-hydroxymandelic acid and PHA is established, and the method plays an important role in further reduction of production cost and improvement of sustainable development.

The invention provides an organic carrier in-root-rhizosphere growth-promoting microbial agent for ecological restoration of degraded grassland as well as a preparation method and application of the microbial agent, and belongs to the technical field of grassland ecological restoration and microbial agents. The organic carrier growth-promoting microbial agent disclosed by the invention is prepared from four kinds of growth-promoting bacteria (PGPE) in roots, four kinds of growth-promoting bacteria (PGPR) in roots and an organic carrier, the PGPE comprises terracotta warrior bacillus K4, bacillus 9-3-1, bacillus deserticola 11-5-4 and halomonas L31, the PGPR comprises serratia rubra 5, serratia rubra 23, cosinia cosinii 11 and enterobacter parabarium 24, and the organic carrier is a carbon-based organic fertilizer, a cattle and sheep manure organic fertilizer or biochar. When the organic carrier growth-promoting microbial agent is applied to the degraded grassland, the biomass and coverage of plant communities can be remarkably improved. The organic carrier growth-promoting microbial agent can be used for ecological restoration of degraded grassland.

The invention relates to the technical field of microorganisms, in particular to a low-temperature-resistant denitrification strain and application thereof in a recirculating aquaculture system. The nitrogen removal bacterial strain is halomonas piezotolans YX98, and the preservation number of the halomonas piezotolans YX98 is CCTCC (China Center for Type Culture Collection) NO: M 20242820. The strain YX98 has an efficient denitrification function and also has the characteristics of low temperature resistance and halophile. The strain is inoculated into simulated wastewater with the temperature of 15 DEG C and the salinity of 20% o, and the degradation rates of ammonia nitrogen, nitrite nitrogen and nitrate nitrogen are as high as 96.15%, 98.20% and 99.99% respectively; when the strain is applied to a salmon and trout circulating mariculture system as a low-temperature fungicide, the NH4 < + >-N and NO2 <->-N degradation function of a moving bed bio-membrane reactor can be enhanced, so that the duration of a function starting period is shortened by 43.75%, and the duration of a function stable period is shortened by 26.32%.

The invention belongs to the technical field of microorganisms, and discloses saline-alkaline tolerant halomonas and application thereof. The Franzmannia qiaohouensis is separated from rhizosphere soil of broom corn millet in saline-alkali soil, the strain number is STM-122, the preservation number is CGMCC No.36349, the classification status is clear through 16S rRNA (ribosomal ribonucleic acid) gene sequence identification and phylogenetic analysis, and the Franzmannia qiaohouensis has the functions of dissolving phosphorus and producing EPS (expandable polystyrene), auxin and siderophore. Through bacterial suspension seed soaking treatment, the germination potential and germination rate of broom corn millet seeds under saline-alkali stress can be remarkably improved, and the indexes of seedling root length, plant height and the like are promoted to be improved; meanwhile, the invention provides a complete preparation method of the bacterial strain fermented bacterial fertilizer, and the viable count of the obtained fermented bacterial fertilizer is greater than or equal to 1 * 10 < 8 > CFU / g. The method can enrich salt-tolerant microbial resources, and provides technical support for planting broom corn millet and other crops in saline-alkali soil.

The present invention relates to a novel strain having an excellent polyhydroxyalkanoate-producing ability and a use thereof. A species of Halomonas ixanhensis, which previously has not been reported to have polyhydroxyalkanoate biosynthesis characteristics, was found to produce polyhydroxyalkanoate. A novel HN-1-3-2 strain that produces polyhydroxyalkanoate in a significantly higher content and concentration than existing polyhydroxyalkanoate-producing strains has been selected, and thus can be used for the purpose of producing polyhydroxyalkanoate.

The present invention provides a method for producing melanin and / or polyhydroxyalkanoate (PHA) materials and / or proteins from microorganisms by means of C1-C1 and / or C2-C2 resources and uses thereof. The Halomonas is modified, so that the Halomonas can be used for producing melanin, and the co-production of extracellular melanin and intracellular PHA (Polyhydroxyalkanoate) and / or protein is realized. According to the method, a platform for sustainable and high-benefit production of melanin, PHA and / or protein by using carbon dioxide is established, and the method plays an important role in further reduction of production cost and improvement of sustainable development.

The invention relates to the technical field of biological materials, in particular to 4-hydroxybutyrate coenzyme A transferase and application thereof. The 4-hydroxybutyrate coenzyme A transferase has an amino acid sequence as shown in SEQ ID NO: 1. According to the 4-hydroxybutyric acid coenzyme A transferase provided by the embodiment of the invention, the proportion of 4-hydroxybutyric acid or 3-hydroxyhexanoic acid in PHA synthesized by eutrophus Rosei and halomonas can be remarkably increased. The engineering modified bacterium containing the 4-hydroxybutyric acid coenzyme A transferase provided by the embodiment of the invention can realize the PHA content of 8.58 g / L, and the molar ratio of 4-hydroxybutyric acid is as high as 46.4%.

The present invention provides a construction method of Halomonas J9U and its derivatives, a PHA preparation method and application, and belongs to the field of genetic engineering technology. The present invention connects the upstream and downstream homology arm sequences of the upp gene with the suicide plasmid pK18mobsacB to obtain a targeting vector pK18Δupp, which is transferred into the aspiring Halomonas J9, and two homologous recombinations occur to obtain J9U. The homologous recombination efficiency of this construction method is extremely high, up to 100%. The maximum yield of PHA produced by the J9UΔxylD‑P8xylA strain using xylose as the sole carbon source is 2.81g / L, and the strain is an advantageous chassis strain in the co-utilization of xylose and glucose. It has superior growth and PHA yield advantages in lignocellulose bioconversion, greatly reducing production costs.

The invention discloses a culture and domestication method of a salt-tolerant heterotrophic nitrification and denitrification microbial agent, and belongs to the technical field of environmental microorganisms. Comprising the following steps: inoculating a heterotrophic nitrification culture medium with a halomonas seed solution, and culturing until a strain reaches a stable growth period to finish domestication. The Halomonas sp. SOB56 strain provided by the invention can be used for effectively removing nitrogen-containing substances in high-salinity wastewater such as mariculture wastewater and the like, and can be adapted to high-salinity wastewater with different salinity through domestication. The removal rates of the strain on ammonia nitrogen and nitrate nitrogen are 99.6% and 94.22% respectively within 36 hours when the strain enters a logarithmic phase under the condition that the temperature is 30 DEG C and the salinity is 5%. The biological nitrogen removal method belongs to the technical field of novel biological nitrogen removal, nitrate nitrogen and nitrite nitrogen are not generated in the ammonia nitrogen removal process, and the biological nitrogen removal method has a good application prospect in actual application of high-salinity wastewater such as mariculture wastewater.

The application discloses a salt-tolerant composite bacterial community, a preparation method thereof and application of the composite bacterial community in high-salt oil sludge remediation. Halomonas venusta The composite bacterial community comprises salt bacillus Acinetobacter calcoaceticus C. acnes CCTCC KB 20081402, and pseudomonas aeruginosa Pseudomonas aeruginosa CCTCC AB 2010174. The application utilizes H. venusta The Ectoine secretion characteristic of the DSM 4743 provides osmotic pressure protection for non-salt-tolerant strains in the composite bacterial community, a Ectoine secretion-transmission-degradation synergistic mechanism is constructed, and the degradation efficiency of petroleum hydrocarbon in a high-salt environment is greatly improved. Experiments show that the petroleum hydrocarbon degradation rate of the composite bacterial community in high-salt oil sludge with a salt content of 35 g / L can reach 43.12% within 7 days. The application is suitable for oil sludge remediation in high-salt environments such as offshore oil fields and saline-alkali soil petroleum contaminated sites, and has important environmental and economic benefits.

This invention provides a method for preparing a high-salinity zinc oxide-resistant nanofluid mediated by Halomonas sp. and its application in enhancing oil recovery. Specifically, it includes: a Halomonas sp. strain CDH-2, with accession number CCTCC NO: M 2025786, which can rapidly grow in high-concentration salt solutions and produce salt-resistant extracellular metabolites. Fermenting and culturing CDH-2 under high-salt conditions yields a fermentation broth with a surface tension below 30 mN / m. Mixing and culturing the obtained fermentation broth with a metal precursor solution yields a high-salinity-resistant metal nanofluid. The resulting metal nanofluid maintains good stable dispersibility in high-salinity water and effectively reduces oil-water interfacial tension. It alters the wettability of oil-wetting surfaces, emulsifies and disperses crude oil, and reduces crude oil viscosity. Therefore, it can be used as an enhanced oil recovery agent in high-salt reservoirs and is widely applied in oilfield flooding, well huff and puff, and heavy oil viscosity reduction.

The invention discloses a salt-tolerant heterotrophic nitrification microbial agent and an application thereof. The salt-tolerant heterotrophic nitrification microbial agent is prepared by compounding seed solutions of a halomonas campparagenia strain LJK10, a marenobacter LJK14 and a halomonas basophilic strain LJK7, and the seed solutions of the halomonas campparagenia strain LJK10, the marenobacter LJK14 and the halomonas basophilic strain LJK7 are mixed to form the salt-tolerant heterotrophic nitrification microbial agent. The volume ratio of the seed solution of the Halomonas campparaganensis strain LJK10 to the seed solution of the Mariobacter LJK14 to the seed solution of the Halomonas basophila LJK7 is (2.5 to 3): (2.0 to 2.5): (1.5 to 2.0). The heterotrophic nitrification microbial agent provided by the invention has salinity tolerance of up to 20%, and still has nitrogen removal efficiency of 100% when the salinity is up to 12%. Therefore, the heterotrophic nitrification microbial agent disclosed by the invention has a huge application prospect in the aspect of biological denitrification of high-salinity mariculture tail water.

The present invention relates to a recombinant halomonas comprising an exogenously introduced xylose metabolic pathway and a metabolic pathway for synthesizing a copolymer of hydroxybutyric acid (HB) and lactic acid (LA), whereby the copolymer can be synthesized from a xylose-containing cellulose hydrolysate, the LA ratio can be controlled by further promoter modification and process optimization, and industrial enlargement is facilitated.

The invention belongs to the technical field of biology, and discloses a polypeptide derived from a deep sea ecological environment, an application of the polypeptide in preparation of an antibacterial preparation and an antibacterial drug. According to the application of the polypeptide disclosed by the invention in the preparation of the antibacterial preparation, the polypeptide comprises one or more of amino acid fragments with sequences shown as SEQ ID NO: 1-9, the compound has good bacteriostatic or bactericidal activity on one or more of escherichia coli, halomonas aeruginosa, acinetobacter picoteri, staphylococcus aureus, linear preristeria, klebsiella pneumoniae and vibrio parahaemolyticus, and has extremely low toxicity on mammalian cells under an effective concentration. The compound has excellent bacteriostatic activity, bacteriostatic broad-spectrum property and biocompatibility, and has very excellent application prospects in multiple fields such as food preservation, agricultural disease prevention and control, feed additives and the like.

The application discloses a kind of salt-tolerant heterotrophic nitrification microbial inoculant and application thereof, the salt-tolerant heterotrophic nitrification microbial inoculant is compounded by seed liquid of halomonas campaniensis strain LJK10, Marinobacterium LJK14 and halomonas alkaliphila LJK7, the volume ratio of the seed liquid of halomonas campaniensis strain LJK10, Marinobacterium LJK14 and halomonas alkaliphila LJK7 is 2.5-3:2.0-2.5:1.5-2.0.The heterotrophic nitrification microbial inoculant of the application tolerates salinity up to 20%, and when salinity is up to 12%, there is still 100% denitrification efficiency.It is shown that the heterotrophic nitrification microbial inoculant of the application has great application prospect in the aspect of biological denitrification of high salinity mariculture tail water.

The invention provides recombinant halomonas, a construction method of the recombinant halomonas and application of the recombinant halomonas in production of polyhydroxyalkanoate. The recombinant halomonas is used for expressing the 2-keto acid decarboxylase or the 2-keto acid decarboxylase mutant. According to the invention, a 2-ketonic acid decarboxylase mediated 3HV synthesis path is constructed, alpha-ketoglutaric acid in tricarboxylic acid circulation can be directly converted into 3-hydroxyvaleryl coenzyme A, de novo synthesis of a 3HV monomer is realized, a conventional carbon source can be efficiently utilized to synthesize 3HV, and the traditional technical bottleneck of dependence on an exogenous propionic acid or valeric acid precursor is broken through; the 3HV with a higher proportion can be obtained by regulating and controlling a metabolic pathway under the condition of not adding an exogenous precursor, so that the fermentation process is remarkably simplified, and the raw material cost and the production complexity are reduced.

The present invention relates to a novel Halomonas lithopenaei strain having good polyhydroxyalkanoate productivity under high salt conditions, and use thereof, a novel Halomonas lithopenaei YBW-3-4-1 strain having been selected, from among Halomonas sp. strains differing as to whether polyhydroxyalkanoate is produced, that produces polyhydroxyalkanoate at notably high volume and concentration under high salt conditions, the strain can be used to produce polyhydroxyalkanoate.

The present invention relates to an aerobic denitrifying Halomonas desiderata Halomonas tianxiuensis LHT6, with a preservation number of CGMCC No. 32312. This strain can tolerate salinity, heavy metal ions and antibiotic stress under low carbon-nitrogen ratio conditions to achieve efficient nitrogen removal, and has application significance in the actual treatment of water bodies such as aquaculture wastewater and pharmaceutical wastewater.

The application provides halomonas and application thereof in preparation of PDRN and related products, and relates to the technical field of biotechnology. Halomonas sp. The application extracts natural PDRN from halomonas for the first time, and provides a new way for production of PDRN. The application further provides a method for extracting natural PDRN from halomonas, which is safe and controllable in the whole closed-loop management from culture of the strain to fermentation, and simple in preparation method, free of extreme reaction conditions and easy for large-scale industrial production.

The invention provides a CRISPR (clustered regularly interspaced short palindromic repeats) transposon gene integration system. The CRISPR transposon gene integration system comprises a vector pQCasTns containing transposase and Cas protein, and a vector pDoner containing a functional sequence, the pQCasTns comprises an operon and a T7 terminator which are connected in sequence; the pDoner comprises a functional sequence recognized by transposase and a crRNA sequence expression module of a targeted genome. The CRISPR transposon system provided by the invention solves a series of problems existing in genome editing of halomonas, and can realize multi-target knockout, high-efficiency deletion of long fragments and high-efficiency integration of exogenous genes.

The present invention relates to a recombinant halomonas for the production of 1, 4-butanediol (1, 4-BDO), which overexpresses a 4-hydroxybutyryl-CoA reductase (adhE) and an ethanol dehydrogenase (yqhD) from E. coli (E. coli), in particular to a recombinant halomonas for the production of 1, 4-butanediol (1, 4-BDO). In order to further improve the production efficiency, glucose-6-phosphate dehydrogenase (zwf) is also overexpressed. The synthesis of the 1, 4-BDO is realized in the halomonas for the first time, the production process is simple, the cost is low, and the application prospect is wide.

The invention relates to the technical field of engineering modification of microorganisms, in particular to recombinant halomonas and application thereof in production of gamma-aminobutyric acid. The invention provides a recombinant halomonas, the recombinant halomonas expresses or overexpresses glutamate decarboxylase and / or glutamic acid / gamma-aminobutyric acid antiporter, and the recombinant halomonas also inactivates gamma-aminobutyric acid transaminase, so that the recombinant halomonas can be used for expressing or overexpressing glutamic acid decarboxylase and / or glutamic acid / gamma-aminobutyric acid antiporter. By culturing the recombinant halomonas disclosed by the invention, efficient production of gamma-aminobutyric acid can be realized, and other products (such as PHA) can be co-produced.

The invention provides a restoration method based on black and odorous bottom mud in-situ chemical oxidation-biological synergy, and belongs to the technical field of environmental engineering. Comprising the following steps: adding a composite chemical oxidant into the black and odorous bottom mud to be repaired, reacting for a preset time, and adding a composite microbial agent into the same area; the complex microbial inoculant comprises microbial flora, growth factors and a composite carrier; wherein the microbial flora comprises pseudomonas putida, thiobacillus denitrificans, halomonas salina and rhodococcus erythropolis. The composite chemical oxidant provides rapid initial pollution reduction, and the composite microbial inoculant has efficient colonization ability, can perform long-term biodegradation and synergistic remediation, ensures the durability of the effect, solves the problem of mutual inhibition of chemical and biological processes, and is environment-friendly. The method is in-situ remediation, large-scale earthwork is not needed, the total cost is reduced, the method is completed mainly by adding chemicals, and the method is easy to apply and popularize in actual engineering.

The invention belongs to the technical field of agriculture, and particularly relates to application of halomonas DY-S025GC05 to improvement of salt tolerance of plants and / or promotion of plant growth. The invention provides an application of halomonas DY-S025GC05 in improving the salt tolerance of plants and / or promoting the growth of the plants, the halomonas DY-S025GC05 is derived from deep-sea sediments below 3000 meters in a ninety-degree Hailing of Indian Ocean, and the strain can obviously improve the salt tolerance of the plants and promote the growth of the plants; at the low temperature (10 DEG C), the strain still can efficiently play a role in improving the growth performance and the salt tolerance of plants, so that a new source is provided for obtaining efficient functional strains for solving soil problems, and the strain has a huge application prospect in the fields of saline-alkali soil improvement and green biological fertilizers.

The invention discloses a method for producing ectoine, which comprises the following steps of: culturing halomonas in a fermentation culture medium, optimizing the culture medium, supplementing materials in two stages and optimizing the C / N ratio of the supplemented materials, so that the ectoine can be produced at low cost and high yield by using the halomonas under low salt concentration, and the yield can reach more than 100g / L.

The invention relates to an adaptive evolution culture method of Halomonas and application of the Halomonas in preparation of PHB, and belongs to the technical field of biology. According to the method, the red algae are pretreated with the acid with the specific concentration, the hydrolysis rate of the red algae can be increased, and more raw materials are provided for PHB preparation. According to the invention, the halomonas is subjected to 35 times of adaptive evolution culture in the red algae pre-hydrolysate, so that the tolerance and transformation capability of the strain to an inhibitor are improved, the prepared culture solution containing the halomonas evolved strain is used for preparing PHB (poly-3-hydroxybutyrate), and the yield of the PHB can be obviously improved. According to the method, the PHB is prepared by fermentation of the Halomonas evolved strain, the red algae are taken as the raw material, the hydrolysate obtained by pre-hydrolysis does not need solid-liquid separation, and fermentation is performed after enzymolysis of cellulase, so that more raw materials can be released, the content of the PHB is further increased, the utilization rate of the red algae is increased, and meanwhile, the preparation process steps are simplified.

The invention relates to the technical field of biological materials, in particular to a production method of engineering modified halomonas and glutamic acid. The engineering modified halomonas does not express glutamate dehydrogenase B, and the engineering modified halomonas overexpresses glutamate dehydrogenase A. The invention further discloses a preparation method of the engineering modified halomonas. According to the engineering modified halomonas provided by the embodiment of the invention, higher cell accumulation density and higher glutamic acid yield are realized at the same time, and the advantage is more obvious especially under a high osmotic pressure fermentation condition.

The invention discloses an oil absorbent felt and application thereof in degradation of grease. The oil absorption felt comprises an oil absorption material and a complex microbial inoculant; the invention relates to a compound bacterial agent, which comprises yarrowia lipolytica (Yarrowia lipolytica), Gordonia bronchialis (Gordonia bronchialis) and Halomonas tianensis (Halomonas tianensis), and is characterized in that the compound bacterial agent comprises the following components: Yarrowia lipolytica (Yarrowia lipolytica (Yarrowia lipolytica), Gordonia bronchialis (Gordonia bronchialis) and Halomonas tianensis (Halomonas tianensis). According to the oil absorbent felt, the degradation rate of grease can be greatly increased, and volatilization of substances harmful to people in the grease is reduced; the device can be used in a household oil cup cleaning scene, and a user does not need to carry out complex cleaning operation on the oil cup; the oil absorption felt can be discarded after single use, and the bottom of the oil cup is still clean and smooth. Through the design of the oil absorption felt, the period of cleaning an oil cup by a user can be greatly prolonged.

The present invention discloses a method for knocking out large DNA fragments in Halomonas using a dual-sgRNA binding RecET system. In the method for knocking out large DNA fragments in Halomonas disclosed by the present invention, the size of the knocked-out large DNA fragment is greater than or equal to 10 kb, and the method comprises: introducing a recombinant vector having the following features 1)-4) into the starting Halomonas to achieve the knockout of the large DNA fragment; 1) capable of transcribing sgRNA1 and sgRNA2, where sgRNA1 and sgRNA2 respectively target both ends of the knocked-out large DNA fragment; 2) containing an upstream homologous arm and a downstream homologous arm of the knocked-out large DNA fragment in the starting Halomonas; 3) capable of expressing Cas9; 4) capable of expressing RecE protein and RecT protein. The present invention solves the problem that large DNA fragment knockout cannot be experimentally carried out in Halomonas at present, and has broad application prospects.

This invention discloses a highly efficient halomonas strain for synthesizing selenium nanoparticles, along with its preparation method and applications, belonging to the field of biotechnology. The halomonas strain CCTCC NO: M 20242266 of this invention exhibits strong tolerance to high concentrations of selenite and can efficiently convert selenite into selenium nanoparticles. The required culture conditions are simple, low-cost, and easy to operate. The selenium nanoparticles produced using this strain are stable and have high bioactivity. The halomonas strain CCTCC NO: M 20242266 or its microbial agents, or the selenium nanoparticles produced using this strain, have broad development prospects in functional agriculture, including crop and animal husbandry, functional food processing, health food processing, and pharmaceutical product processing.