41 results about "Halomonas salina" patented technology

The present invention relates to a novel strain having an excellent polyhydroxyalkanoate-producing ability and a use thereof. A species of Halomonas ixanhensis, which previously has not been reported to have polyhydroxyalkanoate biosynthesis characteristics, was found to produce polyhydroxyalkanoate. A novel HN-1-3-2 strain that produces polyhydroxyalkanoate in a significantly higher content and concentration than existing polyhydroxyalkanoate-producing strains has been selected, and thus can be used for the purpose of producing polyhydroxyalkanoate.

The present invention provides a method for producing melanin and / or polyhydroxyalkanoate (PHA) materials and / or proteins from microorganisms by means of C1-C1 and / or C2-C2 resources and uses thereof. The Halomonas is modified, so that the Halomonas can be used for producing melanin, and the co-production of extracellular melanin and intracellular PHA (Polyhydroxyalkanoate) and / or protein is realized. According to the method, a platform for sustainable and high-benefit production of melanin, PHA and / or protein by using carbon dioxide is established, and the method plays an important role in further reduction of production cost and improvement of sustainable development.

The invention discloses a culture and domestication method of a salt-tolerant heterotrophic nitrification and denitrification microbial agent, and belongs to the technical field of environmental microorganisms. Comprising the following steps: inoculating a heterotrophic nitrification culture medium with a halomonas seed solution, and culturing until a strain reaches a stable growth period to finish domestication. The Halomonas sp. SOB56 strain provided by the invention can be used for effectively removing nitrogen-containing substances in high-salinity wastewater such as mariculture wastewater and the like, and can be adapted to high-salinity wastewater with different salinity through domestication. The removal rates of the strain on ammonia nitrogen and nitrate nitrogen are 99.6% and 94.22% respectively within 36 hours when the strain enters a logarithmic phase under the condition that the temperature is 30 DEG C and the salinity is 5%. The biological nitrogen removal method belongs to the technical field of novel biological nitrogen removal, nitrate nitrogen and nitrite nitrogen are not generated in the ammonia nitrogen removal process, and the biological nitrogen removal method has a good application prospect in actual application of high-salinity wastewater such as mariculture wastewater.

The application discloses a salt-tolerant composite bacterial community, a preparation method thereof and application of the composite bacterial community in high-salt oil sludge remediation. Halomonas venusta The composite bacterial community comprises salt bacillus Acinetobacter calcoaceticus C. acnes CCTCC KB 20081402, and pseudomonas aeruginosa Pseudomonas aeruginosa CCTCC AB 2010174. The application utilizes H. venusta The Ectoine secretion characteristic of the DSM 4743 provides osmotic pressure protection for non-salt-tolerant strains in the composite bacterial community, a Ectoine secretion-transmission-degradation synergistic mechanism is constructed, and the degradation efficiency of petroleum hydrocarbon in a high-salt environment is greatly improved. Experiments show that the petroleum hydrocarbon degradation rate of the composite bacterial community in high-salt oil sludge with a salt content of 35 g / L can reach 43.12% within 7 days. The application is suitable for oil sludge remediation in high-salt environments such as offshore oil fields and saline-alkali soil petroleum contaminated sites, and has important environmental and economic benefits.

This invention provides a method for preparing a high-salinity zinc oxide-resistant nanofluid mediated by Halomonas sp. and its application in enhancing oil recovery. Specifically, it includes: a Halomonas sp. strain CDH-2, with accession number CCTCC NO: M 2025786, which can rapidly grow in high-concentration salt solutions and produce salt-resistant extracellular metabolites. Fermenting and culturing CDH-2 under high-salt conditions yields a fermentation broth with a surface tension below 30 mN / m. Mixing and culturing the obtained fermentation broth with a metal precursor solution yields a high-salinity-resistant metal nanofluid. The resulting metal nanofluid maintains good stable dispersibility in high-salinity water and effectively reduces oil-water interfacial tension. It alters the wettability of oil-wetting surfaces, emulsifies and disperses crude oil, and reduces crude oil viscosity. Therefore, it can be used as an enhanced oil recovery agent in high-salt reservoirs and is widely applied in oilfield flooding, well huff and puff, and heavy oil viscosity reduction.

The present invention relates to a recombinant halomonas comprising an exogenously introduced xylose metabolic pathway and a metabolic pathway for synthesizing a copolymer of hydroxybutyric acid (HB) and lactic acid (LA), whereby the copolymer can be synthesized from a xylose-containing cellulose hydrolysate, the LA ratio can be controlled by further promoter modification and process optimization, and industrial enlargement is facilitated.

The invention belongs to the technical field of biology, and discloses a polypeptide derived from a deep sea ecological environment, an application of the polypeptide in preparation of an antibacterial preparation and an antibacterial drug. According to the application of the polypeptide disclosed by the invention in the preparation of the antibacterial preparation, the polypeptide comprises one or more of amino acid fragments with sequences shown as SEQ ID NO: 1-9, the compound has good bacteriostatic or bactericidal activity on one or more of escherichia coli, halomonas aeruginosa, acinetobacter picoteri, staphylococcus aureus, linear preristeria, klebsiella pneumoniae and vibrio parahaemolyticus, and has extremely low toxicity on mammalian cells under an effective concentration. The compound has excellent bacteriostatic activity, bacteriostatic broad-spectrum property and biocompatibility, and has very excellent application prospects in multiple fields such as food preservation, agricultural disease prevention and control, feed additives and the like.

The present invention relates to a novel Halomonas lithopenaei strain having good polyhydroxyalkanoate productivity under high salt conditions, and use thereof, a novel Halomonas lithopenaei YBW-3-4-1 strain having been selected, from among Halomonas sp. strains differing as to whether polyhydroxyalkanoate is produced, that produces polyhydroxyalkanoate at notably high volume and concentration under high salt conditions, the strain can be used to produce polyhydroxyalkanoate.

The application provides halomonas and application thereof in preparation of PDRN and related products, and relates to the technical field of biotechnology. Halomonas sp. The application extracts natural PDRN from halomonas for the first time, and provides a new way for production of PDRN. The application further provides a method for extracting natural PDRN from halomonas, which is safe and controllable in the whole closed-loop management from culture of the strain to fermentation, and simple in preparation method, free of extreme reaction conditions and easy for large-scale industrial production.

The invention provides a CRISPR (clustered regularly interspaced short palindromic repeats) transposon gene integration system. The CRISPR transposon gene integration system comprises a vector pQCasTns containing transposase and Cas protein, and a vector pDoner containing a functional sequence, the pQCasTns comprises an operon and a T7 terminator which are connected in sequence; the pDoner comprises a functional sequence recognized by transposase and a crRNA sequence expression module of a targeted genome. The CRISPR transposon system provided by the invention solves a series of problems existing in genome editing of halomonas, and can realize multi-target knockout, high-efficiency deletion of long fragments and high-efficiency integration of exogenous genes.

The invention provides a restoration method based on black and odorous bottom mud in-situ chemical oxidation-biological synergy, and belongs to the technical field of environmental engineering. Comprising the following steps: adding a composite chemical oxidant into the black and odorous bottom mud to be repaired, reacting for a preset time, and adding a composite microbial agent into the same area; the complex microbial inoculant comprises microbial flora, growth factors and a composite carrier; wherein the microbial flora comprises pseudomonas putida, thiobacillus denitrificans, halomonas salina and rhodococcus erythropolis. The composite chemical oxidant provides rapid initial pollution reduction, and the composite microbial inoculant has efficient colonization ability, can perform long-term biodegradation and synergistic remediation, ensures the durability of the effect, solves the problem of mutual inhibition of chemical and biological processes, and is environment-friendly. The method is in-situ remediation, large-scale earthwork is not needed, the total cost is reduced, the method is completed mainly by adding chemicals, and the method is easy to apply and popularize in actual engineering.

The invention belongs to the technical field of agriculture, and particularly relates to application of halomonas DY-S025GC05 to improvement of salt tolerance of plants and / or promotion of plant growth. The invention provides an application of halomonas DY-S025GC05 in improving the salt tolerance of plants and / or promoting the growth of the plants, the halomonas DY-S025GC05 is derived from deep-sea sediments below 3000 meters in a ninety-degree Hailing of Indian Ocean, and the strain can obviously improve the salt tolerance of the plants and promote the growth of the plants; at the low temperature (10 DEG C), the strain still can efficiently play a role in improving the growth performance and the salt tolerance of plants, so that a new source is provided for obtaining efficient functional strains for solving soil problems, and the strain has a huge application prospect in the fields of saline-alkali soil improvement and green biological fertilizers.

The invention discloses a method for producing ectoine, which comprises the following steps of: culturing halomonas in a fermentation culture medium, optimizing the culture medium, supplementing materials in two stages and optimizing the C / N ratio of the supplemented materials, so that the ectoine can be produced at low cost and high yield by using the halomonas under low salt concentration, and the yield can reach more than 100g / L.

The invention relates to an adaptive evolution culture method of Halomonas and application of the Halomonas in preparation of PHB, and belongs to the technical field of biology. According to the method, the red algae are pretreated with the acid with the specific concentration, the hydrolysis rate of the red algae can be increased, and more raw materials are provided for PHB preparation. According to the invention, the halomonas is subjected to 35 times of adaptive evolution culture in the red algae pre-hydrolysate, so that the tolerance and transformation capability of the strain to an inhibitor are improved, the prepared culture solution containing the halomonas evolved strain is used for preparing PHB (poly-3-hydroxybutyrate), and the yield of the PHB can be obviously improved. According to the method, the PHB is prepared by fermentation of the Halomonas evolved strain, the red algae are taken as the raw material, the hydrolysate obtained by pre-hydrolysis does not need solid-liquid separation, and fermentation is performed after enzymolysis of cellulase, so that more raw materials can be released, the content of the PHB is further increased, the utilization rate of the red algae is increased, and meanwhile, the preparation process steps are simplified.

The invention discloses an oil absorbent felt and application thereof in degradation of grease. The oil absorption felt comprises an oil absorption material and a complex microbial inoculant; the invention relates to a compound bacterial agent, which comprises yarrowia lipolytica (Yarrowia lipolytica), Gordonia bronchialis (Gordonia bronchialis) and Halomonas tianensis (Halomonas tianensis), and is characterized in that the compound bacterial agent comprises the following components: Yarrowia lipolytica (Yarrowia lipolytica (Yarrowia lipolytica), Gordonia bronchialis (Gordonia bronchialis) and Halomonas tianensis (Halomonas tianensis). According to the oil absorbent felt, the degradation rate of grease can be greatly increased, and volatilization of substances harmful to people in the grease is reduced; the device can be used in a household oil cup cleaning scene, and a user does not need to carry out complex cleaning operation on the oil cup; the oil absorption felt can be discarded after single use, and the bottom of the oil cup is still clean and smooth. Through the design of the oil absorption felt, the period of cleaning an oil cup by a user can be greatly prolonged.

This invention discloses a highly efficient halomonas strain for synthesizing selenium nanoparticles, along with its preparation method and applications, belonging to the field of biotechnology. The halomonas strain CCTCC NO: M 20242266 of this invention exhibits strong tolerance to high concentrations of selenite and can efficiently convert selenite into selenium nanoparticles. The required culture conditions are simple, low-cost, and easy to operate. The selenium nanoparticles produced using this strain are stable and have high bioactivity. The halomonas strain CCTCC NO: M 20242266 or its microbial agents, or the selenium nanoparticles produced using this strain, have broad development prospects in functional agriculture, including crop and animal husbandry, functional food processing, health food processing, and pharmaceutical product processing.

The invention relates to a recombinant halomonas, which comprises an exogenously introduced xylose metabolic pathway and a metabolic pathway for synthesizing a copolymer of hydroxybutyric acid (HB) and lactic acid (LA), so that the copolymer can be synthesized through cellulose hydrolysate containing xylose, the production cost is reduced, and a new thought is provided for green manufacturing.

The invention belongs to the technical field of grassland restoration, and relates to a microbial ecological restoration method for a degenerated typical grassland, which comprises the following steps: selecting the degenerated typical grassland to be treated, ditching, applying a carrier growth-promoting microbial agent, and combining the carrier growth-promoting microbial agent with an arbuscular mycorrhizal fungus agent and / or an azotobacter agent according to the degeneration type. The carrier growth-promoting microbial agent comprises specific strains such as serratia rubra, enterobacter paraphora, cosinia cosinii, bacillus and halomonas. The carrier growth-promoting microbial agent is prepared from the specific strains such as serratia rubra, enterobacter paraphora, cosinia cosinii, bacillus and halomonas. The microbial inoculum is cultured and prepared by being loaded on an organic fertilizer carrier, and the application amount is adjusted according to different degeneration degrees. According to the method, vegetation recovery is remarkably promoted through the synergistic effect of three main microbial functional groups of soil, the biomass and coverage of plant communities are improved, soil nutrients and microbial activity are improved, and the method is suitable for various degeneration types such as grazing degeneration, cradling degeneration and desertification typical grassland; the method has the advantages of being good in remediation effect, environmentally friendly, high in sustainability and the like.

The application provides a recombinant Halomonas, a construction method thereof and application of the recombinant Halomonas in production of polyhydroxyalkanoate. The recombinant Halomonas expresses 2-keto acid decarboxylase or a 2-keto acid decarboxylase mutant. The application constructs a 3HV synthesis path mediated by 2-keto acid decarboxylase, which can directly convert alpha-ketoglutarate in the tricarboxylic acid cycle into 3-hydroxyvaleryl coenzyme A, realizes de novo synthesis of 3HV monomers, can efficiently utilize conventional carbon sources to synthesize 3HV, breaks through the technical bottleneck of traditional dependence on exogenous propionic acid or valeric acid precursors, realizes obtaining of a higher proportion of 3HV under the condition of no need of adding exogenous precursors, and significantly simplifies the fermentation process and reduces raw material cost and production complexity.

The application provides a recombinant bacterium for producing 3-hydroxypropionic acid, and a construction method and application thereof. The recombinant bacterium expresses a halomonas sp. derived accA gene, accB gene and malonyl-CoA reductase. The application fully utilizes the 3HB synthesis pathway of the strain itself, cooperates with the introduced 3-HP synthesis path, realizes high-efficiency synthesis of poly(3-hydroxybutyric acid-3-hydroxypropionic acid) or poly(3,4-dihydroxybutyric acid-3-hydroxypropionic acid) without passing through the intermediate product 3-hydroxypropionic acid aldehyde, and avoids the toxic effect of the intermediate product on cells.

The application belongs to the technical field of microorganisms, and discloses a halophilic halomonas and application thereof. Franzmannia qiaohouensis The halomonas is separated from the millet rhizosphere soil of saline-alkali land, has a strain number STM-122, a preservation number CGMCC No. 36349, is identified and classified by 16S rRNA gene sequence identification and phylogenetic analysis, and has the functions of phosphorus solubilization, EPS production, auxin production and iron carrier production. Through seed treatment by bacterial suspension, the germination energy and germination rate of millet seeds under saline-alkali stress can be significantly improved, and the indexes such as seedling root length and plant height are promoted; meanwhile, a complete preparation method of the fermentation bacterial fertilizer of the strain is provided, and the obtained fermentation bacterial fertilizer has a viable bacterial count ≥1×10 8 CFU / g. The application can enrich the salt-tolerant microbial resources, and provide technical support for the planting of millet and other crops in saline-alkali land.

The invention discloses a complex microbial inoculant capable of degrading grease as well as a preparation method and application of the complex microbial inoculant. The complex microbial inoculant is prepared from Yarrowia lipolytica, Gordonia bronchialis and Halomonas tianensis, and the complex microbial inoculant is prepared from Yarrowia lipolytica, Gordonia bronchialis and Halomonas tianensis. The complex microbial inoculant disclosed by the invention has a relatively high degradation rate on grease, especially on high-concentration grease. The complex microbial inoculant or the grease degradation preparation can be used for degrading kitchen grease, and the microbial degradation method is low in cost, free of secondary pollution risk and excellent in development.

The invention discloses a sewage treatment method, and belongs to the technical field of water, wastewater, sewage or sludge treatment. According to the invention, halotolerant bacillus, halomonas sphaeromonas and an embedding medium containing a nano synergist are combined to finally form a carrier, and an immobilized strain preparation is obtained. When the compound is applied to a high-salt organic wastewater degradation experiment, COD (Chemical Oxygen Demand) in water quality indexes of a compound group is 51.3 mg / L, ammonia nitrogen is 5.9 mg / L, total nitrogen is 10.6 mg / L, and volatile phenol is 0.23 mg / L, so that the compound group is superior to a salt-tolerant bacillus group using a single strain, a stone spring halomonas group using a single strain, a magnesium synergist group using nano magnesium particles and a zinc synergist group using nano zinc particles. Therefore, after the halotolerant bacillus and the halomonas

The application provides an organic carrier endo- rhizosphere growth promoting microbial agent for degraded grassland ecological restoration and a preparation method and application thereof, and belongs to the technical field of grassland ecological restoration and microbial agents. The organic carrier growth promoting microbial agent comprises four endo- rhizosphere growth promoting bacteria (PGPE), four rhizosphere growth promoting bacteria (PGPR) and an organic carrier; the PGPE comprises B. terrigena K4, B. subtilis 9-3-1, B. safensis 11-5-4 and Halomonas sp. L31, the PGPR comprises Serratia marcescens 5, Serratia marcescens 23, Kosakonia koreensis 11 and Enterobacter cloacae 24, and the organic carrier is carbon-based organic fertilizer, cattle and sheep manure organic fertilizer or biochar. The application of the organic carrier growth promoting microbial agent in degraded grassland can significantly improve the biomass and coverage of the plant community. The organic carrier growth promoting microbial agent can be used for the ecological restoration of degraded grassland.

The invention belongs to the technical field of sewage treatment, and particularly relates to a salt-tolerant denitrification composite microbial agent as well as a preparation method and application thereof. The salt-tolerant denitrification complex microbial inoculant is obtained by respectively domesticating salt-tolerant bacillus, salt lake vibrio, halophilic pseudomonas and food organic matter halomonas, performing single-bacterium high-density fermentation and compounding. The complex microbial inoculant disclosed by the invention has relatively strong combination and synergy capabilities, a bacterial strain metabolism function has strong synergy complementarity, the bacterial reproduction rate is high, the nitrogen removal efficiency is high, the production cost is low, the complex microbial inoculant is environment-friendly, pollution-free and excellent and efficient in performance, does not need a complex process during use, is directly put in use, and can effectively degrade organic pollutants, COD, NH3-N and TN in a water body.

The invention relates to the technical field of biological materials, in particular to engineering modified halomonas, a construction method thereof and a method for producing a tissue filling material by using the engineering modified halomonas. The engineering modified halomonas is obtained by overexpressing a phaAB gene (SEQ ID NO: 2) through a promoter porin 59 (SEQ ID NO: 1) in the Halomonas blue phaseensis CYL37, and the engineering modified halomonas is obtained by overexpressing the phaAB gene (SEQ ID NO: 2) through the promoter porin 59 (SEQ ID NO: 1). The material for tissue filling prepared by the embodiment of the invention has better mechanical properties, and can realize the effects of long-acting filling and skin improvement.

The application discloses a heterotrophic nitrification and aerobic denitrification bacteria for treating high-salt and high-ammonia-nitrogen wastewater, and relates to a high-salt and high-ammonia-nitrogen wastewater treatment method. Halomonas sp The bacteria strain is Halomonas sp. Halomonas , named MY-11, belongs to the genus Halomonas, is preserved in the China Center for Type Culture Collection, has a preservation number of CCTCC M 20242923 and a preservation date of December 27, 2024. The bacteria strain is a heterotrophic nitrification and aerobic denitrification bacteria, can efficiently remove inorganic nitrogen pollution in water bodies, has good denitrification performance under high-salt and high-ammonia-nitrogen stress, and has high tolerance to high-salt and high-ammonia-nitrogen. The bacteria strain can efficiently treat nitrogen-containing wastewater under the conditions of 2%-6% salinity and 100 mg / L-400 mg / L ammonia-nitrogen concentration, and the removal efficiency can be higher than 99%. The bacteria strain has great potential in the application of inorganic nitrogen pollution treatment of various water bodies such as breeding and chemical industry wastewater.

The application discloses a kind of recombinant bacillus subtilis and its method for efficiently producing tetrahydropyrimidine using bean dregs hydrolysate, belong to microbial fermentation technical field.First, with bacillus subtilis 168 as host, heterologous import from Halomonas elongate ectABC Gene cluster constructs basic expression strain.Then through CRISPR-Cas9 technology, key genes of competitive metabolic branch hom And dapA Are precisely knocked out, successfully block the conversion of precursor material to threonine, methionine and lysine, significantly improve the carbon flux to the direction of tetrahydropyrimidine synthesis.Not only solve the equipment corrosion problem caused by traditional salt-tolerant bacteria fermentation, but also overcome the conventional engineering bacteria when using bean dregs fermentation byproduct many, low conversion rate bottleneck.Finally, the recombinant strain fermentation 36 h, tetrahydropyrimidine production can reach 28.68 g / L, provide extremely creative solution for the high-value utilization of agricultural waste bean dregs and the low-cost green production of tetrahydropyrimidine.

The invention provides a method for efficiently synthesizing and rapidly detecting PHB through organic solid waste bioconversion, and relates to the technical field of organic solid waste treatment and recycling. The method comprises the following steps: carrying out solid-liquid separation on the organic solid waste, inoculating halomonas hydrothermal in the separated liquid, and carrying out oscillation fermentation; the halomonas hydrotherm is Halomonas hydrotherm Slthf2 ATCC (American Type Culture Collection) BAA-800, and the halomonas hydrotherm is Halomonas hydrotherm Slthf2 ATCC BAA-800. According to the method disclosed by the invention, the organic solid waste is used as a substrate for synthesizing the PHAs by microorganisms, and the PHA is efficiently synthesized by using the Halomonas strain Slthf2ATCC BAA-800 under an open condition through process optimization, so that the culture cost is reduced, and the recycling of the organic solid waste is realized. The invention further provides a Nile red fluorescent staining and flora density combination method for rapidly judging the concentration of the PHB.

The application provides an engineered halomonas and a method for producing acetoin, wherein the halomonas expresses acetyl-lactate synthase and acetyl-lactate decarboxylase, and does not express PHA hydratase and acetoacetyl-CoA or the expressed PHA hydratase and acetoacetyl-CoA are not functional or have reduced function. The engineered halomonas can realize economic, sustainable and efficient production of acetoin, and improve single batch fermentation yield.