64 results about "Halomonas salina" patented technology

The invention discloses a halophilic bacterial agent and a preparation method thereof as well as a biological treatment system fixed with the bacterial agent and application thereof. In the invention, strains are screened from sewage, sludge and soil to be prepared into a bacterial agent, wherein the bacterial agent comprises four strains including Providenciasp. CJ-4, Oceanobcillussp. CJ-10, Brevibacterium epidermidis CJ-12 and Halomonassp. CJ-13. The invention also provides the biological treatment system fixed with the bacterial agent and a method for treating waste water by using the same. The bacterial agent disclosed by the invention has stronger degradation capability on phenolic wastewater, can adapt to high-salt environments and has very good degradation effects under an environment with the salt content of 2-25% and the phenol concentration of 2500 mg/l; and compared with the traditional active sludge method, the bacterial agent has obvious advantages in the performance of degrading phenolic compounds, and the degradation effect on the phenolic compounds in water can reach more than 99.9%.

The invention discloses halomonas TF-1 and application thereof. The strain of the halomonas TF-1 is obtained from an oil-water well sample of the Shengli Oil Field under the conditions of 37 DEG C temperature and ordinary pressure by using a high-salt culture medium containing 6.0% of NaCl through enrichment culture screening, temperature-resistant salt-resistant performance investigation, repeated screening and passage, is named as halomonas sp.TF-1, and the preservation number of the halomonas TF-1 is CGMCC No.10619. The nutrient culture medium of the halomonas TF-1 is prepared from 0.3 mg/L of glucose, 0.3 mg/L of peptone, 0.3 mg/L of yeast powder, 0.27 mg/L of K2HPO4 and 6 mg/L sodium chloride, and a pH value is 7.0 -7.5. The halomonas TF-1 grows with crude oil as a carbon source, the bacterial concentration is greater than 5*108 per milliliter. The emulsification viscosity reduction rate of fermentation liquid is higher than 90%. The recovery ratio improved in a physical simulation experiment is higher than 15%. The average oil increase amount of microbial single well stimulation is more than 250 tons. An averagely prolonged hot washing period of microbial paraffin control is greater than 120d. Therefore, the halomonas TF-1 can be widely applied to a field test of microbial single well treatment.

The invention belongs to the technical field of electric-microbiology collaborative remediation of soil contaminated by petroleum hydrocarbon, and particularly relates to a mixed microbial agent suitable for electric-microbiology collaborative remediation of high-salinity soil contaminated by the petroleum hydrocarbon as well as preparation and application of the mixed microbial agent. The microbial agent is obtained by respectively fermenting and mixing halomonas xianhensis, bacillus licheniformis and bacillus cereus. The combined microbial agent disclosed by the invention is applied to an electric-microbiology collaborative remediation technology; the remediation bottleneck of the high-salinity state of the soil, formed by the remediation process, is broken, functional microbial agent resources are reasonably utilized, degradation of the petroleum hydrocarbon in petroleum oil is effectively realized, and the toxicity of petroleum hydrocarbon organic pollutants in the soil is reduced.

A 3-hydroxypropionic acid(3HP) degradation way of halophilic bacteria is analyzed for the first time by a transcriptomics method, the inventor finds that the 3-hydroxypropionic acid can degrade genesdddA and dddC, and recombination halophilic bacteria TD delta dddA which cannot degrade 3HP is obtained. Based on this, an alcohol dehydrogenase gene AdhP and an aldehyde dehydrogenase gene AldDTD, which are from the halophilic bacteria endogenous and responsible for production and metabolism of the 3HP are further utilized, and a synthetizing biology means is taken for adjusting and controlling the expression level of the metabolic way. Through screening, the recombination halophilic bacteria high in yield of the 3HP is obtained, and the yield and the productivity are respectively 0.93g/g ofthe 1,3-propylene glycol(PDO) and 2.4g/L/h, which are the highest 3HP yield and productivity reported so far.

The invention provides a novel polyhydroxyalkanoate (PHA) tripolymer poly (3-hydroxybutyrate-4-hydroxybutyrate-5-hydroxyvalerate) and construction of a recombinant bacterium for its production, and particularly relates to a microorganism which can be any bacterium, in particular to halomonas. By constructing halomonas for producing the terpolymer, a specific substrate is selected, and the degradable PHA terpolymer is produced through fermentation.

The invention relates to a salt-tolerant Halomonas sp. Strain and application thereof in the field of water purification, the strain is preserved in China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.24127. The strain has good salt tolerance, the optimum growth salinity is 5%, under laboratory conditions, the ammonia nitrogen degradation rate reaches 100% in 24 h when the salinity is 15% or below, and the ammonia nitrogen degradation rate reaches 100% when the salinity is 15% or below. The strain can resist high ammonia nitrogen concentration and high temperature, the ammonia nitrogen degradation rate reaches 89.1% within 72 h for the ammonia nitrogen concentration of 3000 ppm, and when the initial ammonia nitrogen concentration is 100 ppm, the ammonia nitrogen degradation rate reaches 100% within 24 h at the temperature of 30-40 DEG C.

The invention discloses a saccharomyces cerevisiae engineering strain for synthesizing ectoine through fermentation, and belongs to the technical field of biological engineering. According to the invention, saccharomyces cerevisiae is taken as a host, a triple shuttle plasmid pEBS of bacillus subtilis, escherichia coli and saccharomyces cerevisiae is taken as an expression vector, an ectABC gene cluster derived from halomonas elongata is introduced into the saccharomyces cerevisiae, then strategies such as ectA, ectB and ectC single-gene RBS optimization, promoter optimization, single-gene RBS and promoter double optimization and the like are carried out, and efficient biosynthesis of ectoine is realized. The ectoine accumulation amount is up to 2.3 g/L when the recombinant saccharomyces cerevisiae strain constructed by the invention is cultured for 96 hours on a shake flask, and the recombinant saccharomyces cerevisiae strain has potential and wide application value in industry.

The invention discloses a method for synthesizing a biodegradable mulching film from straws and illegal cooking oil by using halomonas sp.ZY-1 under an open and non-sterile condition. Straws are treated to obtain a straw supernatant, and an MS culture medium with the volume ratio of the straw supernatant to illegal cooking oil being 35: 1-40: 1 is prepared. In a sequencing batch reactor (SBR), Halomonas sp.ZY-1 is subjected to aerobic culture with the unsterilized straw supernatant and illegal cooking oil MS culture medium to obtain thalli, and intracellular polyester substances are extracted.Polyester substances are further modified to obtain the mulching film with the tensile strength of 16-18 MPa, the mulching film is deeply buried in cultivated land soil with the depth of 20-30 cm, the 35d weight reduction rate is 40%-50%, and the 35d weight reduction rate is 0.3%-0.5% when the mulching film is placed in dry air. Under the condition that halophilic bacteria are not sterilized, wastes are utilized to synthesize the biological mulching film, so that the production cost is reduced to the maximum extent, and technical support is provided for realizing green ecological cycle and agricultural sustainable development.

The invention belongs to the technical field of nitrogen pollution, and provides halomonas DY-S025GC05 with nitrification and denitrification effects and application of the halomonas DY-S025GC05. TheLatin of the halomonas DY-S025GC05 with nitrification and denitrification effects is Halomonas sp., the strain has been preserved in China General Microbiological Culture Collection Center, the address is Institute of Microbiology, Chinese Academy of Sciences, No.3, Yard 1, West Beichen Road, Chaoyang District, Beijing, the preservation date is May 19th, 2020, and the preservation number is CGMCCNO.19847. The halomonas DY-S025GC05 provided by the invention has double activities of nitrification and denitrification at the same time, and the conversion of nitrification and denitrification functions can be realized by adjusting the oxygen content.

The invention provides a quantitative detection method for halomonas strains by adopting an indirect enzyme-linked immunosordent assay. The quantitative detection method comprises the following steps: (1) preparing an O antigen of halomonas; (2) preparing a polyclonal antibody of the halomonas, namely with the O antigen of the halomonas as an immunizing antigen, immunizing a New Zealand rabbit, and preparing the polyclonal antibody of the halomonas by adopting an existing polyclonal antibody preparation and purification technology; and (3) carrying out an indirect ELISA operation method, namely coating the antigen with an elisa plate, and staying overnight at 4 DEG C, washing the plate, adding confining liquid, washing the plate, and adding the antibody, washing the plate, and adding a second antibody, washing the plate, and carrying out chromogenic reaction, and measuring the light absorption value, namely OD450 at a wavelength of 450nm by using a microplate reader. The quantitative detection method for the halomonas strains by adopting the indirect enzyme-linked immunosordent assay is simple and convenient in operation flow and is easy to operate, the detection sensitivity can reach 105cfu/mL, and antiserum generates no cross reaction with other bacteria, so that the quantitative detection method is a fast and efficient detection method with high sensitivity and strong specificity, promotes the application of probiotics on aquatic products and has the certain practical application significance.

The invention belongs to the technical field of preparation of polyhydroxyalkanoate, and discloses a preparation method and application of polyhydroxyalkanoate. The preparation method comprises the following steps: inoculating a fermentation culture medium with a halomonas strain for fermentation culture; after the fermentation culture is performed for 6-10 hours, the mass percent of the carbon source is controlled to be 1.3-1.6%, and the mass percent of the nitrogen source is controlled to be 0.015-0.05%; after the fermentation is performed for 30 to 40 hours, the mass percent of the nitrogen source is controlled to be 0.01 to 0.015 percent; and after the fermentation culture time is 45-50 hours, stopping controlling the concentration of the nitrogen source. By controlling the concentration of the carbon source, the concentration of the nitrogen source, the adding time and other factors, the preparation method of the polyhydroxyalkanoate improves the conversion rate of the polyhydroxyalkanoate, reduces the usage amount of sodium hydroxide, and also has the advantages of being simple and easy to industrialize.

The invention discloses a Halomonas strain and an application thereof. The strain is a new species in Halomonas, and the preservation number of the strain is CGMCC (China General Microbiological Culture Collection Center) NO.24977. The invention further discloses a preparation method of the Halomonas strain. The strain C2-1-M8 obtained by separation in the invention is a new species different from a known species of Halomonas, and can be used for synthesizing ectoine (Ectoine) in cells. The strain C2-1-M8 can utilize substrates such as D-fructose, glycerol, D-fructose-6-phosphoric acid, L-alanine, L-histamine, L-serine, D-galacturonic acid, D-glucuronic acid, glucosamide, L-lactic acid, D-malic acid, L-malic acid, Tween 40 and the like, and has a broad-spectrum property of the substrates. According to physiological and biochemical experiments, the growth temperature of the strain C2-1-M8 ranges from 4 DEG C to 30 DEG C, the NaCl concentration needed by growth ranges from 1.0% to 17.0%, and the growth pH value ranges from 5.0 to 7.5. Meanwhile, the yield of tetrahydropyrimidine synthesized by the strain C2-1-M8 under the conditions that the temperature is 30 DEG C, the NaCl concentration is 5.0%-9.0% and the pH is 5.5-7.0 is optimal. The strain has a very wide application prospect in biological preparation of ectoine.