39 results about "3-Hydroxypentanoic acid" patented technology

Organisms are provided which express enzymes such as glycerol dehydratase, diol dehydratase, acyl-CoA transferase, acyl-CoA synthetase β-ketothiolase, acetoacetyl-CoA reductase, PHA synthase, glycerol-3-phosphate dehydrogenase and glycerol-3-phosphatase, which are useful for the production of PHAs. In some cases one or more of these genes are native to the host organism and the remainder are provided from transgenes. These organisms produce poly (3-hydroxyalkanoate) homopolymers or co-polymers incorporating 3-hydroxypropionate or 3-hydroxyvalerate monomers wherein the 3-hydroxypropionate and 3-hydroxyvalerate units are derived from the enzyme catalysed conversion of diols. Suitable diols that can be used include 1,2-propanediol, 1,3 propanediol and glycerol. Biochemical pathways for obtaining the glycerol from normal cellular metabolites are also described. The PHA polymers are readily recovered and industrially useful as polymers or as starting materials for a range of chemical intermediates including 1,3-propanediol, 3-hydroxypropionaldehyde, acrylics, malonic acid, esters and amines.

Several novel PHA polymer compositions produced using biological systems include monomers such as 3-hydroxybutyrate, 3-hydroxypropionate, 2-hydroxybutyrate, 3-hydroxyvalerate, 4-hydroxybutyrate, 4-hydroxyvalerate and 5-hydroxyvalerate. These PHA compositions can readily be extended to incorporate additional monomers including, for example, 3-hydroxyhexanoate, 4-hydroxyhexanoate, 6-hydroxyhexanoate or other longer chain 3-hydroxyacids containing seven or more carbons. This can be accomplished by taking natural PHA producers and mutating through chemical or transposon mutagenesis to delete or inactivate genes encoding undesirable activities. Alternatively, the strains can be genetically engineered to express only those enzymes required for the production of the desired polymer composition. Methods for genetically engineering PHA producing microbes are widely known in the art (Huisman and Madison, 1998, Microbiology and Molecular Biology Reviews, 63: 21-53). These polymers have a variety of uses in medical, industrial and other commercial areas.

The invention discloses construction and application of a polygene knockout strain of Halomonas sp. TD01. The invention provides a recombinant strain which is obtained by inactivating one or more genes related to metabolic pathways of propionic acid in the Halomonas sp. TD01 used for producing polyhydroxyalkanoate (PHA). The one or more genes related to metabolic pathways of propionic acid are at least one selected from the group consisting of a coding gene for 2-methylcitrate synthetase, a coding gene for PHA digestive enzyme 1, a coding gene for PHA digestive enzyme 2 and a coding gene for PHA digestive enzyme 3. According to results of experiments in the invention, molecular modification is carried out on the Halomonas sp. TD01 to knock out 2-methylcitrate synthetase PrpC and three PHA digestive enzymes so as to obtain the novel recombinant strain, poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) with more excellent material performance can be highly efficiently produced by utilizing propionic acid, and the proportion of 3-hydroxyvaleric acid monomers in the produced PHBV and the conversion rate of the substrate propionic acid are substantially improved.

This invention relates to a process for the preparation of a 3-hydroxycarboxylic acid from a 3-hydroxynitrile. More specifically, 3-hydroxyvaleronitrile is converted to 3-hydroxyvaleric acid in high yield at up to 100% conversion, using as an enzyme catalyst 1) nitrile hydratase activity and amidase activity or 2) nitrilase activity of a microbial cell. 3-Hydroxyvaleric acid is used as a substitute for ε-caprolactone in the preparation of highly branched copolyester.

The invention discloses recombinant escherichia coli QW102PT and an application thereof in producing poly(3-hydroxybutyrate-3-hydroxyvalerate) (PHBV) by utilizing a single carbon source. The recombinant escherichia coli can be used for efficiently utilizing the single carbon source to synthesize the PHBV, and the mole fraction of 3HV (3-hydroxyvaleric acid) is improved from 0.45 percent to 17.5 percent compared with that of recombinant bacteria DH5 alpha (pBHR68), which has obvious significance to commercial production of the PHBV. According to the invention, the problems that the cost is overhigh as propionic acid is used as an auxiliary carbon source and control strategies during the production process are excessively complicated are solved, and a new concept of synthesizing the PHBV byutilizing a simple carbon source is created.

The invention relates to a synthetic route and a method for preparing (R)-5-(2-(4-fluorophenyl)-5-isopropyl-3-phenyl-4-phenylamino formyl-1H-pyrrole-1-ethyl)-3-hydroxyvalerate. The (R)-5-(2-(4-fluorophenyl)-5-isopropyl-3-phenyl-4-phenylamino formyl-1H-pyrrole-1-ethyl)-3-hydroxyvalerate is obtained through seven steps by using (R)-2,3-dihydroxy-chloropropane as raw material. The method provided bythe invention has low cost, simple operation, and is suitable for industrialization.

The present invention relates to an obtainment process of biodegradable polymers from a citric residue resulting from the processing of orange juice. The polymers obtained are polyesters classified as polyhydroxyalkanoates including, among them the poly(3-hydroxybutyrate) and poly(3-hydroxybutyrate-co-3-hydroxyvalerate). The biodegradable polymer is obtained from the batch culture process or fed batch culture process with or without recirculation of the cells, using as a carbon source the pre-treated pressing liquor and / or the citric molasses. The polyhydroxyalkanoates, herein described, can be used as substitutes of the synthetic polyesters in different areas, including the food, pharmaceutical, medical, agricultural and other areas.