2832 results about "Bacillus sp" patented technology

The invention discloses a microbial compound bacterial fertilizer for improving saline alkali soil and increasing the acting yield. A preparation method of the microbial compound bacterial fertilizer comprises the following steps: preparing a microbial agent containing phosphate-solubilizing bacteria, potassium-solubilizing bacteria, nitrogen-fixing bacteria, bacillus subtilis and actinomycetes; the preparation method comprises the following steps: preparing a sodium alginate solution, adding nano-hydroxyapatite and a microbial agent, uniformly mixing, dropwise adding a calcium chloride solution, and embedding an inner layer to obtain microbial embedded particles; uniformly mixing biochar, humic acid and attapulgite, taking a 2-3% HPMC solution as a binder, and carrying out middle-layer coating, so as to obtain prefabricated microbial fertilizer particles; and coating the chitosan-sodium alginate-nano ZnO outer layer to obtain the microbial compound bacterial fertilizer. The microbial compound bacterial fertilizer disclosed by the invention adopts a multi-layer and multifunctional collaborative design concept, realizes efficient protection and slow release of functional microorganisms in a saline-alkali environment, remarkably improves the physicochemical properties of soil and promotes the growth of crops.

The invention provides bacillus and application thereof, the bacillus is preserved in Guangdong Microbial Culture Collection Center on June 6, 2025, and the preservation number is GDMCC No.66483; one application of the bacillus is to prepare a preparation for promoting growth and / or resisting diseases of plants, and the other application is to use the bacillus for inhibiting microorganisms; the bacillus can effectively and enduringly inhibit the growth of phytopathogen, and can be used as a broad-spectrum antibacterial biological antibacterial agent to enhance the resistance of plants and promote the growth of the plants.

The invention relates to the field of self-repairing concrete, in particular to high-strength self-repairing concrete and a preparation method thereof. The high-strength self-repairing concrete comprises the following substances in parts by weight: 45-60 parts of cement; 100 to 120 parts of recycled coarse aggregate; 65 to 80 parts of fine aggregate; 10 to 20 parts of water; 0.1 to 0.3 part of a water reducing agent; the recycled coarse aggregate is the recycled coarse aggregate immobilized with self-repairing microorganisms, and the self-repairing microorganisms comprise at least one of bacillus subtilis, bacillus megatherium and bacillus pasteurii. The concrete crack self-repairing capability is endowed through the immobilized self-repairing microorganisms. The microorganisms form resting spores in the concrete, when cracks are generated and water permeates into the cracks, the microorganisms are activated and metabolize a calcium source and urea in the surrounding environment, calcium carbonate crystals are generated through the mechanism that the microorganisms induce carbonate precipitation, the cracks are filled, and the structural integrity is recovered. The process does not need external intervention, and the mechanical structure and mechanical properties of the concrete structure can be remarkably improved.

The invention discloses a bio-organic fertilizer for repairing heavy metal cadmium in soil and a preparation method of the bio-organic fertilizer, and belongs to the technical field of organic fertilizers. The organic fertilizer is prepared from the following raw materials in parts by weight: 7-12 parts of a compound microbial agent, 30-40 parts of modified charcoal, 3-8 parts of sodium alginate, 6-8 parts of a chelating agent and 60-80 parts of decomposed edible fungus residues. The compound microbial agent is prepared from potassium-solubilizing paenibacillus mucilaginosus MSSW02, pseudomonas plecoglossicida and bacillus aryabhattai; the chelating agent is composed of calcium humate and nano-hydroxyapatite according to the mass ratio of 100: (3-5). The multi-component synergistic effect is achieved, the content of heavy metal in soil is effectively reduced, especially the content of heavy metal Cd, meanwhile, rich organic matter is contained, the physical and chemical properties of the soil can be remarkably improved, the water and fertilizer retention capacity of the soil is improved, and crop growth is promoted; the remediation effect on medium-light cadmium polluted farmland soil is good, and the growth promoting effect on crops is remarkable.

The invention belongs to the technical field of microorganisms, particularly relates to rice endophytic bacillus velezensis AN6 and application thereof, and particularly provides bacillus velezensis AN6 which is classified and named as bacillus velezensis and has the preservation number of CCTCC (China Center for Type Culture Collection) NO: M 20231886. The invention provides a novel rice endophytic bacillus velezensis strain AN6, through separation, screening, identification and experimental verification, it is found that the strain shows broad-spectrum resistance to various phytopathogens including rice bacterial leaf blight, rice blast and the like, the strain has the capacity of inhibiting various phytopathogens and has the capacity of adsorbing heavy metal, and the strain has the advantages of being capable of inhibiting various phytopathogens and capable of being used as a novel rice endophytic bacillus velezensis strain AN6. The method is of great significance to biological control of rice diseases and development of green agriculture.

The invention relates to the technical field of animal feed preparation, in particular to a functional feed additive for ruminants and a preparation method of the functional feed additive for the ruminants, and the functional feed additive for the ruminants is characterized by consisting of the following components in percentage by mass: probiotics which are saccharomycetes, bacillus subtilis and enterococcus faecalis; an enzyme preparation, xylanase and protease; prebiotics are added; a microcapsule coating material; a binder; the balance is a carrier; the probiotics and the enzyme preparation are wrapped in the chitosan / sodium alginate composite film through a microcapsule coating technology, adverse factors such as temperature, humidity and illumination in the production, transportation and storage process can be effectively isolated, meanwhile, in the rumen low-pH, strong-protease and complex-microorganism environment, the coating layer can slowly release and protect active ingredients, and the effect of preventing and treating the probiotics and the enzyme preparation is achieved. Effective content and activity during final feeding are ensured, consistent growth promotion can be kept in different varieties, weights and feeding stages, and production benefits and feeding stability are improved.

The invention discloses a broad-spectrum biocontrol strain water bacillus barren ZG18 and application thereof, and belongs to the technical field of plant disease control, and the broad-spectrum biocontrol strain water bacillus barren ZG18 is characterized in that the water bacillus barren ZG18 is water bacillus barren and is preserved in the China General Microbiological Culture Collection Center, and the preservation number of the water bacillus barren ZG18 is CGMCC (China General Microbiological Culture Collection Center). The preservation address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing, China; the preservation date is September 6, 2024; the preservation number is CGMCC (China General Microbiological Culture Collection Center) The biocontrol bacterium strain disclosed by the invention has a relatively strong inhibition effect on pathogenic fusarium sporotrichum, fusarium graminearum, botrytis cinerea, rhizoctonia solani and corticosis cucurbitae, the prevention and treatment effect of the strain ZG18 on tobacco target leaf spot reaches up to 51.34%, the prevention and treatment effect of the strain ZG18 on tobacco damping-off in a pot experiment is 65.91%, and the strain ZG18 has a promoting effect on tobacco growth.

The invention discloses a preparation method of a curing agent with biological self-healing capability, which comprises the following steps: preparing a bacillus pasteurii bacterial solution, preparing the bacillus pasteurii bacterial solution into an endophytic spore solution, immersing polyvinyl alcohol fibers into a sodium alginate / endophytic spore solution, taking out the polyvinyl alcohol fibers, immediately immersing the polyvinyl alcohol fibers into a calcium acetate solution, drying the polyvinyl alcohol fibers to obtain core fibers with a hydrogel coating, and curing the core fibers with the hydrogel coating to obtain the curing agent with biological self-healing capability. The preparation method comprises the following steps: taking magnesium phosphate cement as a raw material, immediately immersing the magnesium phosphate cement into a chloroform solution of a polystyrene and polylactic acid polymer blend, drying to obtain a coated microbial liquid, premixing urea and glucose, doping the premixed urea and glucose into the magnesium phosphate cement, uniformly stirring, adding the coated microbial liquid, and uniformly stirring to obtain the product. According to the invention, phosphate cement is adopted as the curing agent, has the advantages of high strength, short setting time, strong durability, low alkalinity, good biocompatibility and the like, and compared with the curing agent in the prior art, the curing agent not only has a good curing effect, but also has a crack self-healing function, and is especially suitable for reinforcing foundation soil.

The invention discloses a complex microbial inoculant for coupling straw decomposition and soil carbon sequestration and a preparation method thereof, the complex microbial inoculant can rapidly promote degradation of lignocellulose of straw in soil by compounding bacillus subtilis, bacillus licheniformis, pseudomonas aeruginosa and the like, provides a basic material energy source for activity of soil microorganisms, and has the advantages of simple preparation process and low cost. Meanwhile, corresponding microorganisms achieve long-term storage of organic carbon through humus stabilization and mineral substance combined carbon (MAOC) stabilization, the storage efficiency of the organic carbon is remarkably improved through nano Fe3O4, and under the synergistic effect of all strains in the complex microbial inoculant, the carbon loss of straw returning can be effectively reduced, the carbon immobilization capacity of soil is improved, and the soil quality is improved. The organic carbon content in the soil is increased.

The invention discloses a saline-alkaline tolerant growth-promoting bacillus velezensis AH216 and application thereof, and belongs to the technical field of microbiology and biologica.Bacillus velezensis AH216 is preserved in the China Center for Type Culture Collection (CCTCC), and the preservation number is CCTCC No: M 20241496. The saline-alkaline tolerant growth-promoting bacillus velezensis AH216 has the advantages that the saline-alkaline tolerant growth-promoting bacillus velezensis AH216 can be used for preparing saline-alkaline tolerant growth-promoting bacillus velezensis AH216; the bacillus velezensis AH216 provided by the invention can inhibit the growth of pathogenic bacteria of cotton fusarium wilt, and has the growth promoting functions of dissolving potassium, generating indoleacetic acid, cellulase, protease, siderophore and the like; meanwhile, the bacillus velezensis AH216 can tolerate a highly saline-alkaline environment, can improve agronomic characters such as the plant height, the biomass and the leaf area in the corn seedling stage in common soil and saline-alkaline soil, and can promote the development of a corn root system.

The invention provides a special charcoal-based slow-release fertilizer for sandy soil as well as a preparation method and application thereof, and belongs to the technical field of fertilizers. The special charcoal-based slow-release fertilizer for the sandy soil is prepared from amino modified biomass charcoal, urea, urea formaldehyde, monoammonium phosphate, potassium sulfate, polyglutamic acid, fulvic acid, clay, attapulgite, a compound microbial agent and sodium alginate. The compound microbial agent is prepared from halomonas (ACCC 11681), bacillus subtilis (ACCC 60429) and bacillus mucilaginosus (ACCC 10094), and the compound microbial agent is prepared from bacillus subtilis (ACCC 60429) and bacillus mucilaginosus (ACCC 10094). The invention further provides a preparation method and application of the special charcoal-based slow-release fertilizer for the sandy soil. The special charcoal-based slow-release fertilizer for the sandy soil can remarkably improve the water and fertilizer retention capacity of the sandy soil, improve the farmland productivity of the sandy soil, save fertilizer and water input and improve the yield and quality of crops.

The invention discloses bacillus velezensis YB-1652, a biocontrol microbial inoculum and application of the biocontrol microbial inoculum, and aims to solve the technical problem of lack of effective biological control measures for continuous accumulation of peanut phenolic acid autotoxic substances and pathogens in continuous cropping. According to the application, a bacillus velezensis YB-1652 strain is separated and screened from rhizosphere soil of continuous cropping peanuts, and the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.36152. The strain has the capability of efficiently degrading phenolic acid compounds, relieves continuous cropping obstacles and improves the soil environment; the compound has a relatively strong inhibition effect on southern blight and root rot of peanuts, and the occurrence rate of soil-borne diseases is reduced; meanwhile, peanut growth can be promoted; the bacillus amyloliquefaciens has siderophore, cellulase, beta-glucanase, amylase power and protease production capacity. The YB-1652 strain and the preparation thereof can provide technical support for restoration of peanut continuous cropping obstacles, provide bacterial source guarantee for prevention and treatment of peanut soil-borne diseases, replace or reduce use of chemical pesticides and improve food and ecological environment safety, and have good economic and social benefits.

The invention relates to the technical field of agricultural microorganisms, and particularly discloses bacillus vallismortis LRB-5, a biocontrol microbial agent, a bacterial fertilizer and application of the bacillus vallismortis LRB-5, the biocontrol microbial agent and the bacterial fertilizer, the bacillus vallismortis LRB-5 is preserved in China General Microbiological Culture Collection Center on January 25, 2018, the preservation number is CGMCC No.15306, and the bacillus vallismortis LRB-5 is classified and named as bacillus vallismortis. The bacillus vallismortis LRB-5 provided by the invention can inhibit the growth of phytopathogen, promote the growth and development of arabidopsis thaliana and apples, and also can alleviate successive cropping obstacles of the apples.

The invention relates to the technical field of environmental microorganisms, in particular to high-temperature-resistant bacillus subtilis and application thereof. The invention discloses a high-temperature-resistant bacillus, which is named as bacillus subtilis HY-1, has been preserved in China General Microbiological Culture Collection Center (CGMCC) on October 17, 2024, and has a preservation number of CGMCC No.32250. The invention further discloses a preparation method of the high-temperature-resistant bacillus. The invention further discloses a culture method of the bacillus, a liquid fungicide prepared from the bacillus and application of the bacillus in kitchen waste biogas residue compost and straw degradation. The high-temperature-resistant bacillus disclosed by the invention is derived from a compost indigenous strain, can reduce the inhibition effect of other indigenous bacteria, and has relatively good degradation capability and high-temperature-resistant performance on cellulose. The method has a wide application prospect in the field of kitchen waste biogas residue resourceful treatment.

The invention relates to the technical field of agricultural fertilizers, discloses a seaweed extract-functional microorganism compound slow-release fertilizer and a preparation method thereof, and solves the problems in the background art. The seaweed extract is a compound containing enteromorpha polysaccharide and alginic acid extract; the functional microorganism is a complex microbial inoculant composed of phosphate solubilizing bacteria, nitrogen-fixing bacteria, phosphate solubilizing bacteria and bacillus; the slow-release carrier is prepared by coating the seaweed extract and the functional microorganisms with sodium alginate-chitosan microcapsules; the signal molecule is selected from at least one of polyamine substances and jasmonic acid methyl ester; the fertilizer has the advantages that the utilization rate of the fertilizer can be increased, growth and reproduction of soil microorganisms are promoted, the stress resistance of crops is improved, and the requirements of different soil types and planting environments can be met.

The invention relates to the technical field of biomineralization, and particularly discloses an alkali-resistant urease-producing microbial flora for biomineralization of construction joints and a preparation method of the alkali-resistant urease-producing microbial flora. The microbial flora disclosed by the invention is prepared from the following strains with relative abundance: 4.4 to 5.2 percent of bacillus, 0.39 to 0.46 percent of enterococcus, 4.8 to 5.7 percent of alcaligenes, 0.50 to 0.58 percent of cellulomonas, 22.4 to 24.2 percent of lysobacter, 1.7 to 2.4 percent of pseudomonas, 10.8 to 11.9 percent of Savagea, 15.1 to 16.2 percent of sarcina, 30.8 to 32.2 percent of chloridomonas and 0.41 to 0.48 percent of bacillus. The alkali-resistant microbial compound flora with high urease activity in the limited oxygen and normal environment can effectively overcome the defects that urease microorganisms are low in mineralization efficiency under the anoxic condition, and the strength of an obtained consolidated body is not high.

The invention belongs to the field of microorganisms, and particularly relates to banana endophytic bacillus velezensis JDB15 and application thereof. The novel endophytic bacillus velezensis JDB15 is separated from banana plants, has the characteristics of efficient inhibition of Foc4, growth promotion of bananas and broad-spectrum antagonism of various pathogenic bacteria, can inhibit the morbidity of corn southern leaf blight, tomato fusarium wilt, tobacco gray mold and peach anthracnose, has the effects of inhibiting the morbidity of various pathogenic fungi, and has the advantages of high efficiency, low cost and the like. And a good broad-spectrum effect is achieved. Meanwhile, the strain can kill nematodes, has wide biocontrol bacterium activity, can be developed deeply subsequently, and provides a sustainable and green solution for prevention and treatment of banana wilt and other diseases and insect pests.

The invention discloses a microbial compound feed additive, which is prepared from the following ingredients in parts by weight: 35 to 45 parts of traditional Chinese medicine residues, 25 to 35 parts of soybean meal, 15 to 25 parts of bran, 8 to 12 parts of corn flour, 0.3 to 0.7 part of selenium-enriched yeast, 8 to 12 parts of aspergillus niger spore powder, 10 to 15 parts of bacillus subtilis, 6 to 9 parts of lactobacillus plantarum and 5 to 8 parts of candida utilis. The invention relates to the technical field of feed additives, through the synergistic effect of the aspergillus niger, the bacillus subtilis, the lactobacillus plantarum and the candida utilis, the aspergillus niger can generate various enzymes, and decomposition of raw materials and release of nutritional ingredients are facilitated; the bacillus subtilis can enhance the immunity of livestock and poultry and inhibit the growth of harmful bacteria; lactobacillus plantarum can regulate intestinal microecological balance and promote digestion and absorption; candida utilis is rich in protein and vitamins and provides nutrition for livestock and poultry, the four components cooperate with each other, the function limitation of a single strain is broken through, and the health level and production performance of livestock and poultry are comprehensively improved.

The invention relates to the technical field of microorganisms and bio-organic fertilizers, and particularly discloses a bio-organic fertilizer for preventing and treating rhizome diseases of horseradish and application of the bio-organic fertilizer, the bio-organic fertilizer comprises the following components: component A: a bacillus velezensis microbial inoculum with the preservation number of CCTCC NO: M 2022923 and a trichoderma yunnanense microbial inoculum with the preservation number of CCTCC NO: M 2025645; the component B is prepared from soybean meal, vinegar residues, charcoal, wollastonite powder and allicin. The biological organic fertilizer disclosed by the invention can be used for increasing the content of growth hormones such as salicylic acid (SA), jasmonic acid (JA) and ethylene (ET) and phytoalexin as well as the activity of chitinase and beta-1, 3-glucanase of a wasabi root system, and has a relatively good prevention and treatment effect on a black heart disease caused by infection of phoma wabi Yokog and a soft rot disease caused by infection of pectobacterium carotovorum subsp. Carotovorum (Pcc). The biological organic fertilizer disclosed by the invention has a relatively good prevention and treatment effect on the soft rot disease caused by infection of the phoma wabi Yokog and a relatively good prevention and treatment effect on the soft rot disease caused by infection of the phoma wabi subsp. Carotovorum (Pcc).

Optimization of the formula of a culture medium for industrially producing a Bacillus velezensis TCS001 fermentation broth and culture process conditions therefor. By using the culture medium and the culture process conditions, not only can the fermentation production cost be reduced, but also the effective viable bacterium count and the spore count in the Bacillus velezensis TCS001 fermentation broth can be improved, so that the fermentation broth can be used for industrial production and market promotion and application. Provided is a lipopeptide-compound-containing mixture separated from a Bacillus velezensis TCS001 fermentation broth. By further optimizing the formula of the culture medium for the Bacillus velezensis TCS001 fermentation broth and the culture fermentation process conditions, the output and yield of lipopeptide compounds in the fermentation broth are successfully improved, providing an efficient and cost-effective solution for the control of plant diseases.

The invention relates to the field of alfalfa planting, in particular to a synthetic flora for breaking dormancy of alfalfa seeds and promoting growth of seedlings and application of the synthetic flora. The invention provides a strain of sinorhizobium meliloti NFB5, the preservation number is CGMCC NO.33618, and the sinorhizobium meliloti NFB5 can break dormancy of alfalfa seeds and promote growth of alfalfa seedlings and root systems. According to the method, sinorhizobium meliloti NFB5 is compounded with one or two of screened salt-tolerant brevibacillus brevis EB3 and bacillus PS06 to obtain a synthetic flora, and alfalfa seeds are soaked in a bacterial solution containing the synthetic flora, so that the number of hard seeds can be remarkably reduced, the germination rate and germination potential of the seeds can be remarkably improved, and the germination rate of the seeds can be remarkably increased. Therefore, the synthetic flora can well break the dormancy of the alfalfa seeds, does not cause loss to the seeds, and does not reduce the quality of the seeds. In addition, the synthetic flora also has a significant growth promoting effect, and can significantly improve the plant height, root length, stem weight and root weight of seedlings.

The invention relates to the technical field of soil function improvement, in particular to a rice field soil recarburization and capacity expansion method based on a charcoal-based organic fertilizer. The method comprises the following steps: crushing a biomass raw material, pyrolyzing and cooling to obtain biochar; immersing the biochar in a functional bacteria solution containing actinomycetes and bacillus for activating treatment to obtain modified biochar; mixing the modified biochar and decomposed straw, composting and fermenting to obtain organic fertilizers with different particle sizes; the large-particle-size organic fertilizer is applied to deep soil, the soil is covered with soil after straw is laid on the soil, and the small-particle-size organic fertilizer is applied to surface soil. Through layered application, the large-particle-size biochar repairs the deep soil structure and expands the carbon storage capacity, and meanwhile, the carbon stability is improved through carbon-nitrogen synergistic metabolism driven by functional bacteria, so that the carbon immobilization efficiency is improved, meanwhile, soil recarburization and expansion are achieved, the contradiction between carbon sequestration and structure degradation and nutrient imbalance in a traditional recarburization and expansion technology is solved, and the soil recarburization and expansion efficiency is improved. Synergic improvement of a carbon sink function and agricultural productivity is promoted.

The invention discloses a decomposition preparation containing bacillus licheniformis as well as a preparation method and application thereof, and belongs to the technical field of microorganisms. The decomposition preparation provided by the invention comprises bacillus licheniformis and Thermus arcuatus. The bacillus licheniformis is preserved in China General Microbiological Culture Collection Center (CGMCC) on June 27, 2025, the preservation address is No.3, No.1 Yard, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC No. 35039. The bacillus licheniformis is separated from coastal saline-alkali soil, has high temperature resistance and salt resistance, and can secrete protease and lipase at the same time. After the bacillus licheniformis and the Thermus arcuatus are mixed, the synergistic effect is remarkable, the duck-derived red mud is effectively promoted to be converted into humic acid, and high-value resource utilization of the duck-derived red mud is achieved.

The invention relates to bacillus subtilis, a biocontrol inoculant prepared from the bacillus subtilis and application of the biocontrol inoculant, and belongs to the technical field of biological inoculants. The bacillus provided by the invention is bacillus subtilis and is named as KC2, the preservation unit is China General Microbiological Culture Collection Center, the preservation address is Institute of Microbiology, Chinese Academy of Sciences, No.3, No.1 Yard, Beichen West Road, Chaoyang District, Beijing, the number of the preservation center is CGMCC No.31372, and the preservation time is July 19, 2024. The bacillus KC2 disclosed by the invention has a certain bacteriostatic effect on different pathogenic bacteria regardless of living bacteria, supernate or cell lysis solution. The bacillus KC2 is high in living ability and wide in application range of nutrient sources, temperature, humidity and pH value. The compound has no pathogenicity to fruits and vegetables, has good tolerance to broad-spectrum bactericides, and has a great application prospect.

The invention discloses preparation of a trichoderma composite flora and application of the trichoderma composite flora in prevention and control of crop fusarium wilt and improvement of soil, and relates to the field of agricultural production, and the composite flora is prepared by mixing trichoderma asperellum (5 * 10 < 7 > CFU / L), streptomyces (108 CFU / L), Chinese monospora (108 CFU / L), ensifer adhesion (108 CFU / L), pseudomonas nitroreducens (108 CFU / L) and paenibacillus alvei (108 CFU / L) according to the volume ratio of 6: 1: 1: 1: 1: 1: 1: 1 (V: V). According to the composite flora in the scheme, the composite flora is used for promoting growth of cucumber plants, and compared with a clear water control group (Foc), the fresh weight of cucumbers is increased by 55.51%, the stem diameter is increased by 12.76%, and the plant height is increased by 34.25%. The composite flora can also effectively prevent and treat fusarium wilt of cucumbers, and the prevention and treatment effect reaches 71.43%. Compared with the Trichoderma asperellum FJ035 treated group, the Trichoderma asperellum FJ035 has the advantages that the Trichoderma asperellum FJ035 is better than the Trichoderma asperellum FJ035 which is independently applied in the aspects of promoting the growth of cucumber plants and preventing and treating cucumber fusarium wilt, and compared with the Trichoderma asperellum FJ035 treated group, the control effect is improved by 56.25%. Meanwhile, the composite flora can be used for improving soil and preventing and treating multi-crop fusarium wilt.

The invention relates to the technical field of compound fertilizers, and provides a slow-release compound fertilizer for corn and a preparation method of the slow-release compound fertilizer. The fertilizer is prepared from the following raw material components: 35 to 40 parts of thermoplastic resin coated urea, 400 to 450 parts of potassium chloride compound fertilizer, 200 to 300 parts of humic acid coated urea, 10 to 12 parts of zinc sulfate, 30 to 45 parts of large granular urea, 100 to 150 parts of microencapsulated bacillus subtilis granules and 10 to 15 parts of polylactic acid coated borax. According to the invention, borax is coated by a polylactic acid coating technology, so that the release speed of the boron element is effectively delayed, the boron element is ensured to be continuously released from the elongation stage of corn to the large bell mouth stage (20-30 days after sowing), and the problem of premature loss of the boron element in the prior art is solved.

The invention belongs to the technical field of health-care products, and particularly relates to a fermented composition beneficial to enhancing immunity and a preparation method thereof. The fermentation composition for improving immunity is prepared by taking yeast beta-glucan, an elderberry extract, glucoraphanin, sea cucumber peptide, inulin and a traditional Chinese medicine zymolyte as fermentation raw materials, firstly inoculating bacillus coagulans and staphylococcus xylosus for aerobic fermentation, and then inoculating bifidobacterium longum for anaerobic fermentation, the aerobic fermentation stage and the anaerobic fermentation stage have a synergistic effect, so that the fermentation speed can be increased, the fermentation time can be shortened, and richer nutrient substances can be provided, and therefore, the composition has an excellent effect of enhancing the immunity; the fermentation composition is applied to preparation of capsules helpful for enhancing immunity, is helpful for enhancing human immunity, and can solve the problem that active substances in existing health-care products are difficult to effectively absorb by human bodies, so that the effect of enhancing immunity is influenced.

The invention relates to the technical field of microorganisms and particularly discloses bacillus velezensis for producing tetramethylpyrazine and application of the bacillus velezensis, the preservation number of the strain is CCTCC NO: M20232058, and the bacillus velezensis is obtained by separating and purifying from high-temperature Daqu of Maotai-flavor liquor in the liquor industry of Jin-brand Maotai Town in Guizhou. The bacillus velezensis disclosed by the invention can produce 1875.4 mg / L of tetramethylpyrazine by performing shake flask fermentation on a liquid culture medium for 7 days, and the tetramethylpyrazine can reach 4810.8 mg / L by optimizing a culture medium formula and culture conditions. The bacillus velezensis is applied to small-scale brewing of Maotai-flavor liquor, the content of tetramethylpyrazine in the Maotai-flavor liquor reaches 34.5 + / -1.4 mg / L and is increased by 325.93% compared with a contrast, and the bacillus velezensis can be used for increasing the content of tetramethylpyrazine in the Maotai-flavor liquor.

The invention discloses bacillus velezensis XF-8 and application of the bacillus velezensis XF-8 in prevention and treatment of tomato bacterial wilt caused by Ralstonia solanacearum, and belongs to the technical field of microorganisms. The bacillus velezensis XF-8 capable of antagonizing Ralstonia solanacearum is obtained through separation and screening, and it is found that the bacillus velezensis XF-8 also has an excellent inhibition effect on various pathogenic fungi and shows broad-spectrum antibacterial activity of the bacillus velezensis XF-8. The bacillus velezensis XF-8 has a good inhibition effect on tomato bacterial wilt, and the control effect of the bacillus velezensis XF-8 in potted tomatoes reaches 74.25%. Results of the invention show that the bacillus velezensis XF-8 has a great application prospect in prevention and treatment of tomato bacterial wilt, and a good biocontrol resource is provided for research and development of a biocontrol agent for tomato bacterial wilt.

The invention relates to the technical field of deep processing of cattle spleen, in particular to cattle spleen active polypeptide capable of enhancing immunity and a preparation method and a functional product thereof. The preparation method comprises the following steps: mincing cattle spleen, adding water, uniformly mixing, heating and curing; adding bacillus licheniformis alkaline protease to carry out hydrolysis for the first time; adding papain and bacillus subtilis flavourzyme into the first hydrolysate to carry out hydrolysis for the second time; and performing enzyme deactivation on the second hydrolysate, and sequentially performing fishy smell removal, decolorization, filtration, concentration and drying treatment to obtain the cattle spleen polypeptide. The cattle spleen polypeptide prepared by the invention can obviously enhance the immune response of a body and play a role in enhancing immunity, can be applied to a functional product for enhancing immunity as a functional factor, has the advantages of low equipment requirement, simple process, easiness in operation, high extraction efficiency and the like, is suitable for industrial production, and has a wide application prospect. The scheme of the invention has good application and economic prospects.