3580 results about "Bacillus sp" patented technology

The invention discloses a microbial compound bacterial fertilizer for improving saline alkali soil and increasing the acting yield. A preparation method of the microbial compound bacterial fertilizer comprises the following steps: preparing a microbial agent containing phosphate-solubilizing bacteria, potassium-solubilizing bacteria, nitrogen-fixing bacteria, bacillus subtilis and actinomycetes; the preparation method comprises the following steps: preparing a sodium alginate solution, adding nano-hydroxyapatite and a microbial agent, uniformly mixing, dropwise adding a calcium chloride solution, and embedding an inner layer to obtain microbial embedded particles; uniformly mixing biochar, humic acid and attapulgite, taking a 2-3% HPMC solution as a binder, and carrying out middle-layer coating, so as to obtain prefabricated microbial fertilizer particles; and coating the chitosan-sodium alginate-nano ZnO outer layer to obtain the microbial compound bacterial fertilizer. The microbial compound bacterial fertilizer disclosed by the invention adopts a multi-layer and multifunctional collaborative design concept, realizes efficient protection and slow release of functional microorganisms in a saline-alkali environment, remarkably improves the physicochemical properties of soil and promotes the growth of crops.

The invention provides bacillus velezensis NJ157 and application thereof, and relates to the technical field of biological pesticides. The bacillus velezensis NJ157 can resist saline and alkaline, has a good inhibition effect on ralstonia solanacearum, rhizoctonia solanacearum, fusarium moniliforme and phytophthora capsici, and can effectively prevent and treat tomato bacterial wilt, root rot and fruit rot. The bacillus velezensis NJ157 disclosed by the invention also has relatively strong capability of producing protease, cellulase and IAA (Indoleacetic Acid), and has a relatively good growth promoting effect on tomatoes, and a fungicide prepared from the biocontrol bacterium does not cause pollution to the ecological environment in a use process, and is pollution-free. By using the biocontrol bacterium disclosed by the invention, the dosage of other chemical pesticides can be reduced, the prevention and control cost of tomato diseases is reduced, the tomato growth is promoted, and the biocontrol bacterium has a good popularization value.

The invention provides bacillus and application thereof, the bacillus is preserved in Guangdong Microbial Culture Collection Center on June 6, 2025, and the preservation number is GDMCC No.66483; one application of the bacillus is to prepare a preparation for promoting growth and / or resisting diseases of plants, and the other application is to use the bacillus for inhibiting microorganisms; the bacillus can effectively and enduringly inhibit the growth of phytopathogen, and can be used as a broad-spectrum antibacterial biological antibacterial agent to enhance the resistance of plants and promote the growth of the plants.

The invention discloses bacillus velezensis GX0003742 and biocontrol application thereof, and belongs to the technical field of microorganism application. The invention discloses bacillus velezensis GX0003742 with a broad-spectrum bacteriostatic effect, which is preserved in the China General Microbiological Culture Collection Center, the preservation address is No.3, No.1 yard, Beichen West Road, Chaoyang District, Beijing, the preservation date is September 14, 2024, and the preservation number is CGMCC No.31981. The bacillus velezensis GX0003742 has the advantages that the bacillus velezensis GX0003742 can be used for preparing the bacillus velezensis GX0003742; the strain can obviously inhibit growth of colletotrichum basjoo mycelia and obviously reduce occurrence of postharvest diseases of bananas, has an inhibiting effect on various pathogenic bacteria, has a good insecticidal effect on auricular bean root-knot nematode, and provides a new strain resource for biological prevention and control of plant diseases.

The invention relates to bacillus velezensis SMB1 and application thereof in prevention and treatment of plant diseases, and belongs to the technical field of biological prevention and treatment of plant diseases. The strain is preserved in the China Center for Type Culture Collection on November 27, 2024, the preservation address is Luojia mountain, Luojia mountain, eight road, Wuchang District, Wuhan City, Hubei Province, and the preservation number is CCTCC NO: M20242647. The bacillus velezensis SMB1 provided by the invention has a remarkable bacteriostatic effect on plant pathogenic fungi, plant pathogenic oomycetes, plant pathogenic bacteria and animal pathogenic bacteria, has an extremely good broad-spectrum bacteriostatic function, has a great application potential in biological control of plant diseases, can reduce the use of chemical pesticides, and has a good application prospect. The pesticide residue in agricultural products is reduced, the environment is protected, and the urgent demand on pollution-free agricultural product production at present is met.

The invention relates to bacillus, a biocontrol inoculant prepared from the bacillus and application of the biocontrol inoculant, and belongs to the technical field of biological inoculants. The bacillus provided by the invention is bacillus subtilis and is named as ZZ1, the preservation unit is China General Microbiological Culture Collection Center, the preservation address is Institute of Microbiology, Chinese Academy of Sciences, No.3, No.1 Yard, Beichen West Road, Chaoyang District, Beijing, the preservation center is numbered as CGMCC No.31374, and the preservation time is July 19, 2024. The bacillus ZZ1 disclosed by the invention has a certain bacteriostatic effect on different pathogenic bacteria regardless of living bacteria, supernate or cell lysis solution. The bacillus ZZ1 is high in living ability and wide in application range of nutrient sources, temperature, humidity and pH value. The compound has no pathogenicity to fruits and vegetables, has good tolerance to broad-spectrum bactericides, and has a great application prospect.

The invention relates to the field of self-repairing concrete, in particular to high-strength self-repairing concrete and a preparation method thereof. The high-strength self-repairing concrete comprises the following substances in parts by weight: 45-60 parts of cement; 100 to 120 parts of recycled coarse aggregate; 65 to 80 parts of fine aggregate; 10 to 20 parts of water; 0.1 to 0.3 part of a water reducing agent; the recycled coarse aggregate is the recycled coarse aggregate immobilized with self-repairing microorganisms, and the self-repairing microorganisms comprise at least one of bacillus subtilis, bacillus megatherium and bacillus pasteurii. The concrete crack self-repairing capability is endowed through the immobilized self-repairing microorganisms. The microorganisms form resting spores in the concrete, when cracks are generated and water permeates into the cracks, the microorganisms are activated and metabolize a calcium source and urea in the surrounding environment, calcium carbonate crystals are generated through the mechanism that the microorganisms induce carbonate precipitation, the cracks are filled, and the structural integrity is recovered. The process does not need external intervention, and the mechanical structure and mechanical properties of the concrete structure can be remarkably improved.

The invention discloses a bio-organic fertilizer for repairing heavy metal cadmium in soil and a preparation method of the bio-organic fertilizer, and belongs to the technical field of organic fertilizers. The organic fertilizer is prepared from the following raw materials in parts by weight: 7-12 parts of a compound microbial agent, 30-40 parts of modified charcoal, 3-8 parts of sodium alginate, 6-8 parts of a chelating agent and 60-80 parts of decomposed edible fungus residues. The compound microbial agent is prepared from potassium-solubilizing paenibacillus mucilaginosus MSSW02, pseudomonas plecoglossicida and bacillus aryabhattai; the chelating agent is composed of calcium humate and nano-hydroxyapatite according to the mass ratio of 100: (3-5). The multi-component synergistic effect is achieved, the content of heavy metal in soil is effectively reduced, especially the content of heavy metal Cd, meanwhile, rich organic matter is contained, the physical and chemical properties of the soil can be remarkably improved, the water and fertilizer retention capacity of the soil is improved, and crop growth is promoted; the remediation effect on medium-light cadmium polluted farmland soil is good, and the growth promoting effect on crops is remarkable.

The invention provides a bacillus subtilis LU5 and a lignocellulose degrading flora as well as a culture method and application thereof, and belongs to the technical field of microorganisms. According to the invention, a strain is screened from surface soil of composted straws and identified as bacillus subtilis, the strain is combined with bacillus velezensis SS6 and trichoderma longibrachiatum according to a specific proportion, co-culture is carried out in a specific culture medium, a flora capable of degrading lignocellulose is obtained, all microorganisms of the flora are in synergistic symbiosis, and the effect of degrading lignocellulose is achieved. The produced various cellulases have a synergistic effect, so that the aim of degrading lignocellulose is fulfilled. And by adopting the flora provided by the invention, the efficiency and the stability of the lignocellulose degradation process are remarkably improved.

The invention belongs to the technical field of microorganisms, particularly relates to rice endophytic bacillus velezensis AN6 and application thereof, and particularly provides bacillus velezensis AN6 which is classified and named as bacillus velezensis and has the preservation number of CCTCC (China Center for Type Culture Collection) NO: M 20231886. The invention provides a novel rice endophytic bacillus velezensis strain AN6, through separation, screening, identification and experimental verification, it is found that the strain shows broad-spectrum resistance to various phytopathogens including rice bacterial leaf blight, rice blast and the like, the strain has the capacity of inhibiting various phytopathogens and has the capacity of adsorbing heavy metal, and the strain has the advantages of being capable of inhibiting various phytopathogens and capable of being used as a novel rice endophytic bacillus velezensis strain AN6. The method is of great significance to biological control of rice diseases and development of green agriculture.

The invention relates to the technical field of animal feed preparation, in particular to a functional feed additive for ruminants and a preparation method of the functional feed additive for the ruminants, and the functional feed additive for the ruminants is characterized by consisting of the following components in percentage by mass: probiotics which are saccharomycetes, bacillus subtilis and enterococcus faecalis; an enzyme preparation, xylanase and protease; prebiotics are added; a microcapsule coating material; a binder; the balance is a carrier; the probiotics and the enzyme preparation are wrapped in the chitosan / sodium alginate composite film through a microcapsule coating technology, adverse factors such as temperature, humidity and illumination in the production, transportation and storage process can be effectively isolated, meanwhile, in the rumen low-pH, strong-protease and complex-microorganism environment, the coating layer can slowly release and protect active ingredients, and the effect of preventing and treating the probiotics and the enzyme preparation is achieved. Effective content and activity during final feeding are ensured, consistent growth promotion can be kept in different varieties, weights and feeding stages, and production benefits and feeding stability are improved.

The invention discloses a broad-spectrum biocontrol strain water bacillus barren ZG18 and application thereof, and belongs to the technical field of plant disease control, and the broad-spectrum biocontrol strain water bacillus barren ZG18 is characterized in that the water bacillus barren ZG18 is water bacillus barren and is preserved in the China General Microbiological Culture Collection Center, and the preservation number of the water bacillus barren ZG18 is CGMCC (China General Microbiological Culture Collection Center). The preservation address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing, China; the preservation date is September 6, 2024; the preservation number is CGMCC (China General Microbiological Culture Collection Center) The biocontrol bacterium strain disclosed by the invention has a relatively strong inhibition effect on pathogenic fusarium sporotrichum, fusarium graminearum, botrytis cinerea, rhizoctonia solani and corticosis cucurbitae, the prevention and treatment effect of the strain ZG18 on tobacco target leaf spot reaches up to 51.34%, the prevention and treatment effect of the strain ZG18 on tobacco damping-off in a pot experiment is 65.91%, and the strain ZG18 has a promoting effect on tobacco growth.

The invention discloses a preparation method of a curing agent with biological self-healing capability, which comprises the following steps: preparing a bacillus pasteurii bacterial solution, preparing the bacillus pasteurii bacterial solution into an endophytic spore solution, immersing polyvinyl alcohol fibers into a sodium alginate / endophytic spore solution, taking out the polyvinyl alcohol fibers, immediately immersing the polyvinyl alcohol fibers into a calcium acetate solution, drying the polyvinyl alcohol fibers to obtain core fibers with a hydrogel coating, and curing the core fibers with the hydrogel coating to obtain the curing agent with biological self-healing capability. The preparation method comprises the following steps: taking magnesium phosphate cement as a raw material, immediately immersing the magnesium phosphate cement into a chloroform solution of a polystyrene and polylactic acid polymer blend, drying to obtain a coated microbial liquid, premixing urea and glucose, doping the premixed urea and glucose into the magnesium phosphate cement, uniformly stirring, adding the coated microbial liquid, and uniformly stirring to obtain the product. According to the invention, phosphate cement is adopted as the curing agent, has the advantages of high strength, short setting time, strong durability, low alkalinity, good biocompatibility and the like, and compared with the curing agent in the prior art, the curing agent not only has a good curing effect, but also has a crack self-healing function, and is especially suitable for reinforcing foundation soil.

The invention relates to bacillus velezensis and application thereof in preparation of a multifunctional straw decomposition agent, and belongs to the technical field of microorganisms. The bacillus velezensis has the functions of producing cellulase, producing xylanase, degrading abamectin, decomposing inorganic phosphorus and degrading straws; when straw is returned to the field, the multifunctional straw decomposition agent provided by the invention not only can accelerate the decomposition speed of straw, decompose abamectin residues in soil and improve the soil ecology, but also can promote the growth of pepper plants and enhance the stress resistance of the pepper plants, and has a good comprehensive application effect.

The invention discloses bacillus velezensis capable of tolerating various environmental pollution and promoting growth and application of the bacillus velezensis. A plant endophyte bacillus velezensis L381 strain is identified in wild rice, and the strain is high in environmental adaptability, can grow in the environment with the temperature of 15-40 DEG C and the pH value of 5-8, and can tolerate the NaCl concentration of 4%; the rice growth promoting agent has a good growth promoting function, can secrete auxin IAA, solubilize phosphorus, dissolve potassium and secrete siderophores, and can effectively promote rice growth. Meanwhile, the strain has stress resistance to environmental stress such as heavy metals and antibiotics, and tetracycline can be degraded; the strain has a wide antibacterial spectrum, and can be used as a biocontrol bacterium for preventing and treating various plant diseases. The plant endophyte L381 can adapt to variable temperature conditions and variable soil environments in the natural environment, has very strong practical application potential, not only improves the functions of bacillus velezensis, but also widens the application range of the strain.

The invention discloses a complex microbial inoculant for coupling straw decomposition and soil carbon sequestration and a preparation method thereof, the complex microbial inoculant can rapidly promote degradation of lignocellulose of straw in soil by compounding bacillus subtilis, bacillus licheniformis, pseudomonas aeruginosa and the like, provides a basic material energy source for activity of soil microorganisms, and has the advantages of simple preparation process and low cost. Meanwhile, corresponding microorganisms achieve long-term storage of organic carbon through humus stabilization and mineral substance combined carbon (MAOC) stabilization, the storage efficiency of the organic carbon is remarkably improved through nano Fe3O4, and under the synergistic effect of all strains in the complex microbial inoculant, the carbon loss of straw returning can be effectively reduced, the carbon immobilization capacity of soil is improved, and the soil quality is improved. The organic carbon content in the soil is increased.

The invention relates to the related technical field of extraction of active ingredients in roselle calyx, and particularly provides an extract based on roselle oil and roselle polypeptide as well as an extraction method and application of the extract. The extraction method comprises the following steps: S1, sequentially performing crushing treatment and supercritical fluid extraction treatment on roselle calyx to obtain a roselle oil extract and flower residues; s2, the roselle residues are sequentially subjected to fermentation treatment and enzymolysis treatment, and a roselle polypeptide extract is obtained; wherein a strain used in the fermentation treatment is selected from at least one of saccharomyces cerevisiae, bacillus subtilis and lipomyces starkeyi; an enzyme used in the enzymolysis treatment is selected from at least one of acid protease and compound protease; the extract comprises the roselle oil extract and the roselle polypeptide extract. The extraction method provided by the invention improves the extraction rate of active ingredients extracted from roselle calyx.

The invention discloses a saline-alkaline tolerant growth-promoting bacillus velezensis AH216 and application thereof, and belongs to the technical field of microbiology and biologica.Bacillus velezensis AH216 is preserved in the China Center for Type Culture Collection (CCTCC), and the preservation number is CCTCC No: M 20241496. The saline-alkaline tolerant growth-promoting bacillus velezensis AH216 has the advantages that the saline-alkaline tolerant growth-promoting bacillus velezensis AH216 can be used for preparing saline-alkaline tolerant growth-promoting bacillus velezensis AH216; the bacillus velezensis AH216 provided by the invention can inhibit the growth of pathogenic bacteria of cotton fusarium wilt, and has the growth promoting functions of dissolving potassium, generating indoleacetic acid, cellulase, protease, siderophore and the like; meanwhile, the bacillus velezensis AH216 can tolerate a highly saline-alkaline environment, can improve agronomic characters such as the plant height, the biomass and the leaf area in the corn seedling stage in common soil and saline-alkaline soil, and can promote the development of a corn root system.

The invention discloses an algae-controlling bioactive preparation as well as a preparation method and application thereof. The preparation method comprises the following steps: inoculating bacillus velezensis HY16 in a bioactive substance fermentation culture medium and culturing; the culture temperature is 35-40 DEG C, and the culture time is 32-48 hours. The algae-controlling bioactive preparation provided by the invention has good capability of inhibiting growth of microcystis aeruginosa, and the algae-lysing rate of the algae-controlling bioactive preparation on microcystis aeruginosa in an aquarium system can reach 97% or above. The algae-controlling bioactive preparation prepared from the bacillus velezensis HY16 fermentation active liquid is stable in property, safe, environment-friendly and simple to apply, can be used for effectively and biologically controlling and repairing an aquarium water body with microcystis aeruginosa, is remarkable in effect, and has good market application prospect and value.

The invention relates to bacillus, a biocontrol inoculant prepared from the bacillus and application of the biocontrol inoculant, and belongs to the technical field of biological inoculants. The bacillus provided by the invention is bacillus subtilis and is named as QY2, the preservation unit is China General Microbiological Culture Collection Center, the preservation address is Institute of Microbiology, Chinese Academy of Sciences, No.3, No.1 Yard, Beichen West Road, Chaoyang District, Beijing, the number of the preservation center is CGMCC No.31373, and the preservation time is July 19, 2024. The bacillus QY2 disclosed by the invention has a certain bacteriostatic effect on different pathogenic bacteria regardless of living bacteria, supernate or cell lysis solution. The bacillus QY2 is high in living ability and wide in application range of nutrient sources, temperature, humidity and pH value. The compound has no pathogenicity to fruits and vegetables, has good tolerance to broad-spectrum bactericides, and has a great application prospect.

The invention belongs to the technical field of microbial pesticides, and particularly relates to application of bacillus velezensis Bv-6 in prevention and treatment of plant viruses. The invention aims to provide a new choice for biological control of plant virus diseases. According to the technical scheme, the invention relates to application of bacillus velezensis Bv-6 in prevention and treatment of plant virus diseases, and the preservation number of the Bv-6 is CCTCC (China Center for Type Culture Collection) No: M20191106. The invention finds that the bacillus velezensis Bv-6 fermentation liquor and the wettable powder of the bacillus velezensis Bv-6 fermentation liquor can be used for effectively preventing and treating plant virus diseases caused by tobacco mosaic virus (Tobacco mosaic virus) and turnip mosaic virus (Turnip mosaic virus) for the first time.

The invention provides a special charcoal-based slow-release fertilizer for sandy soil as well as a preparation method and application thereof, and belongs to the technical field of fertilizers. The special charcoal-based slow-release fertilizer for the sandy soil is prepared from amino modified biomass charcoal, urea, urea formaldehyde, monoammonium phosphate, potassium sulfate, polyglutamic acid, fulvic acid, clay, attapulgite, a compound microbial agent and sodium alginate. The compound microbial agent is prepared from halomonas (ACCC 11681), bacillus subtilis (ACCC 60429) and bacillus mucilaginosus (ACCC 10094), and the compound microbial agent is prepared from bacillus subtilis (ACCC 60429) and bacillus mucilaginosus (ACCC 10094). The invention further provides a preparation method and application of the special charcoal-based slow-release fertilizer for the sandy soil. The special charcoal-based slow-release fertilizer for the sandy soil can remarkably improve the water and fertilizer retention capacity of the sandy soil, improve the farmland productivity of the sandy soil, save fertilizer and water input and improve the yield and quality of crops.

The invention discloses bacillus velezensis YB-1652, a biocontrol microbial inoculum and application of the biocontrol microbial inoculum, and aims to solve the technical problem of lack of effective biological control measures for continuous accumulation of peanut phenolic acid autotoxic substances and pathogens in continuous cropping. According to the application, a bacillus velezensis YB-1652 strain is separated and screened from rhizosphere soil of continuous cropping peanuts, and the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.36152. The strain has the capability of efficiently degrading phenolic acid compounds, relieves continuous cropping obstacles and improves the soil environment; the compound has a relatively strong inhibition effect on southern blight and root rot of peanuts, and the occurrence rate of soil-borne diseases is reduced; meanwhile, peanut growth can be promoted; the bacillus amyloliquefaciens has siderophore, cellulase, beta-glucanase, amylase power and protease production capacity. The YB-1652 strain and the preparation thereof can provide technical support for restoration of peanut continuous cropping obstacles, provide bacterial source guarantee for prevention and treatment of peanut soil-borne diseases, replace or reduce use of chemical pesticides and improve food and ecological environment safety, and have good economic and social benefits.

The invention relates to a salt-tolerant bacillus and application thereof, and belongs to the technical field of microbial fertilizers, the salt-tolerant bacillus is preserved in China General Microbiological Culture Collection Center (CGMCC) on July 10, 2024, and the preservation number is CGMCC No.31260; the strain is relatively strong in saline-alkaline tolerance, has the capabilities of dissolving potassium, decomposing cellulose, degrading protein and dissolving organophosphorus, can antagonize various plant pathogenic bacteria such as Erwinia persicae, fusarium oxysporum and fusarium fusiforme, can generate a good growth promoting effect on corn in a salt-free or salt-containing environment, and is very wide in application prospect.

The invention relates to the technical field of agricultural microorganisms, and particularly discloses bacillus vallismortis LRB-5, a biocontrol microbial agent, a bacterial fertilizer and application of the bacillus vallismortis LRB-5, the biocontrol microbial agent and the bacterial fertilizer, the bacillus vallismortis LRB-5 is preserved in China General Microbiological Culture Collection Center on January 25, 2018, the preservation number is CGMCC No.15306, and the bacillus vallismortis LRB-5 is classified and named as bacillus vallismortis. The bacillus vallismortis LRB-5 provided by the invention can inhibit the growth of phytopathogen, promote the growth and development of arabidopsis thaliana and apples, and also can alleviate successive cropping obstacles of the apples.

The invention belongs to the technical field of plant disease control, and particularly relates to bacillus velezensis and application thereof. The bacillus velezensis strain is separated and screened from rhizosphere soil of wheat in a main wheat area in Hebei Province, the growth characteristics and physiological and biochemical characteristics of the bacterial colony are identified, then 16S rDNA sequencing is carried out, Blast homology comparison is carried out in a GenBank database, the bacterial strain is identified to be bacillus velezensis through the analysis, and the bacillus velezensis strain is identified to be bacillus velezensis. The fungicide containing the bacillus velezensis has a good prevention and treatment effect on wheat stem rot through seed coating.

The invention relates to the technical field of environmental microorganisms, in particular to high-temperature-resistant bacillus subtilis and application thereof. The invention discloses a high-temperature-resistant bacillus, which is named as bacillus subtilis HY-1, has been preserved in China General Microbiological Culture Collection Center (CGMCC) on October 17, 2024, and has a preservation number of CGMCC No.32250. The invention further discloses a preparation method of the high-temperature-resistant bacillus. The invention further discloses a culture method of the bacillus, a liquid fungicide prepared from the bacillus and application of the bacillus in kitchen waste biogas residue compost and straw degradation. The high-temperature-resistant bacillus disclosed by the invention is derived from a compost indigenous strain, can reduce the inhibition effect of other indigenous bacteria, and has relatively good degradation capability and high-temperature-resistant performance on cellulose. The method has a wide application prospect in the field of kitchen waste biogas residue resourceful treatment.

The invention relates to the technical field of agricultural fertilizers, discloses a seaweed extract-functional microorganism compound slow-release fertilizer and a preparation method thereof, and solves the problems in the background art. The seaweed extract is a compound containing enteromorpha polysaccharide and alginic acid extract; the functional microorganism is a complex microbial inoculant composed of phosphate solubilizing bacteria, nitrogen-fixing bacteria, phosphate solubilizing bacteria and bacillus; the slow-release carrier is prepared by coating the seaweed extract and the functional microorganisms with sodium alginate-chitosan microcapsules; the signal molecule is selected from at least one of polyamine substances and jasmonic acid methyl ester; the fertilizer has the advantages that the utilization rate of the fertilizer can be increased, growth and reproduction of soil microorganisms are promoted, the stress resistance of crops is improved, and the requirements of different soil types and planting environments can be met.

The invention discloses bacillus subtilis Gxunw-30 and application thereof, and belongs to the technical field of agricultural microorganisms. The bacillus subtilis Gxunw-30 provided by the invention is preserved in Guangdong Microbial Culture Collection Center on October 12, 2024, and the preservation number of the bacillus subtilis Gxunw-30 is GDMCC No: 65245. The bacillus subtilis Gxunw-30 provided by the invention has the capability of inhibiting plant pathogens in a broad spectrum, and can be used for antagonizing a variety of plant pathogens such as helminthosporium triticum, trichosporon pseudoacaceae, hechiense fusarium, fusarium chlamydosporum, cryphonectria parasitica, fusarium pseudograminearum, botryosphaeria dothidea and the like. The strain has the properties of decomposing organophosphorus, producing protease, producing ammonia, producing indole and fixing nitrogen, and has the biological properties of plant growth promotion and the like. The recombinant bacterium also contains a gene for coding an antibacterial substance, and has the capability of synthesizing the antibacterial peptides of iturin, surfactin, fengmycin and bacilysin, and the recombinant bacterium also contains a gene for coding the antibacterial substance and has the capability of synthesizing the antibacterial peptides of iturin, surfactin, fengmycin and bacilysin. The strain has the effects of preventing diseases and promoting growth, provides a good biocontrol resource for prevention and treatment of plant diseases, and has a relatively good application value for research and development of plant growth-promoting preparations and biocontrol microbial agents.

The invention provides a southern red soil dry land deacidification soil conditioner and a preparation method thereof, and belongs to the technical field of soil conditioners. The preparation method comprises the following steps: embedding bacillus subtilis, bacillus licheniformis and bacillus amyloliquefaciens to prepare bacterial microspheres, adding the bacterial microspheres into gel formed by carboxymethyl chitosan and silk fibroin peptide to prepare sustained-release bacterial microsphere gel, uniformly mixing the sustained-release bacterial microsphere gel with porous silicon dioxide loaded with basalt and charcoal, and drying to prepare the acid-reducing soil conditioner for the southern red soil dry land. The acid-reducing soil conditioner for the southern red soil dry land not only improves the pH value of the soil, but also can loosen the soil and improve the air permeability of the soil to a certain extent, and promotes the absorption of crops to moisture and nutrient elements by improving the physical and chemical properties of the soil, so that the normal growth of the crops is ensured, and the acid-reducing soil conditioner has a wide application prospect.