418 results about "Bacterial wilt" patented technology

The invention relates to the technical field of microorganisms, in particular to application of a cladosporium spp. Strain and a synthetic flora thereof in plant growth promotion and bacterial wilt prevention and treatment, the cladosporium spp. HX2 is a new strain, has a good symbiotic relationship with tomatoes, has a good prevention effect on tomato bacterial wilt, and has a good application prospect in plant growth promotion and bacterial wilt prevention and treatment. After the bacterial strain is mixed with congeneric strains LJ1, MS2 and LC3 to prepare a cladosporium bacterial agent, the growth-promoting and disease-resisting capabilities of the bacterial agent are enhanced, and experiments show that the synthetic flora has a good growth-promoting function on tomatoes and fresh ginger, the yield of the fresh ginger is remarkably increased, and the growth vigor of tomato plants is improved; meanwhile, the synthetic flora also has a good prevention and treatment effect on bacterial wilt of tomatoes, fresh ginger and tobaccos, and can improve the bacterial wilt resistance of plants, so that the strain HX2 and the synthetic flora prepared from the strain HX2 can be used as an anti-microbial agent or a raw material for preparing a bio-organic fertilizer in the later period.

The invention provides a Pseudomonas protegens P150 strain and an application of the Pseudomonas protegens P150 strain, and belongs to the technical field of biocontrol bacteria. The defensive pseudomonas P150 is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC No.34520, the preservation time is May 13, 2025, and the preservation address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The defensive pseudomonas P150 disclosed by the invention has the effects of inhibiting various pathogenic bacteria, producing enzymes, promoting growth and preventing and treating tomato bacterial wilt and soybean pustule. The microbial inoculum prepared by adopting the biocontrol bacterium can reduce the dosage of chemical pesticides and the prevention and control cost of plant diseases, is green and pollution-free, can be used for preventing and controlling tomato bacterial wilt and soybean pustule, and has a relatively good popularization value.

The invention relates to the technical field of microorganisms, in particular to application of Cladosporium LC3 in crop growth promotion and bacterial wilt resistance, the strain LC3 belongs to Cladosporium and Cladosporium immonda species, the strain is separated from Guangxi sugarcane rhizosphere soil, experimental evaluation screening finds that the strain has a good growth promotion function on tomatoes and fresh ginger, and the strain can be applied to crop growth promotion and bacterial wilt resistance. The yield of ginger is remarkably increased, and the growth vigor of tomato plants is effectively improved; meanwhile, the strain has a good prevention and treatment effect on tomato and ginger bacterial wilt and has good plant bacterial wilt resistance, and screening verification of the strain provides a new fungicide and a raw material for later-period growth of an anti-microbial agent or a biological organic fertilizer.

The invention provides Yunnan golden camellia root endophytic fungi and application thereof, and relates to the technical field of crop disease control. The Yunnan camellia nitidissima root endophytic fungus is Lecanicillium vase JHC098 and is preserved in the China Center for Type Culture Collection, the preservation number is CCTCC NO: M 2025749, and a liquid fermentation liquor crude extract of the Lecanicillium vase can be applied to prevention and treatment of crop diseases. The invention overcomes the defects in the prior art, and the crude extract of the liquid fermentation liquor of the endophytic fungus Lecanicillium viculare JHC098 at the root of Yunnan golden camellia can effectively inhibit the growth of pathogenic bacteria such as citrus canker and tobacco bacterial wilt, thereby achieving the purpose of certain biological control.

The invention discloses bacillus velezensis XF-8 and application of the bacillus velezensis XF-8 in prevention and treatment of tomato bacterial wilt caused by Ralstonia solanacearum, and belongs to the technical field of microorganisms. The bacillus velezensis XF-8 capable of antagonizing Ralstonia solanacearum is obtained through separation and screening, and it is found that the bacillus velezensis XF-8 also has an excellent inhibition effect on various pathogenic fungi and shows broad-spectrum antibacterial activity of the bacillus velezensis XF-8. The bacillus velezensis XF-8 has a good inhibition effect on tomato bacterial wilt, and the control effect of the bacillus velezensis XF-8 in potted tomatoes reaches 74.25%. Results of the invention show that the bacillus velezensis XF-8 has a great application prospect in prevention and treatment of tomato bacterial wilt, and a good biocontrol resource is provided for research and development of a biocontrol agent for tomato bacterial wilt.

The invention discloses Burkholderia paradensis and application thereof, and relates to the technical field of microorganisms, the Burkholderia paradensis S3-22L provided by the invention is preserved in China General Microbiological Culture Collection Center (CGMCC), the strain preservation number is GGMCC NO: 31325, the preservation date is July 16, 2024, the preservation number is CGMCC NO: 31325, the preservation number is CGMCC NO: 31325, the preservation number is CGMCC NO: 31325, the preservation number is CGMCC NO: 31325, the preservation number is CGMCC NO: 31325, the preservation number is CGMCC NO: 31325, the preservation number is CGMCC NO: 31325, and the preservation number is CGMCC NO: 31325. The 16S rDNA (ribosomal deoxyribonucleic acid) sequence of the Burkholderia paradensis S3-22L is as shown in SEQ ID NO. 1; the strain obtained through screening can dissolve insoluble inorganic phosphorus and mineralize organic phosphorus, can also produce IAA and siderophore and secrete hydrolytic enzymes such as phosphatase, has an inhibition effect on growth and reproduction of ralstonia solanacearum, has an important application effect on prevention and treatment of plant bacterial wilt, and is harmless to the environment and human beings.

The invention discloses a multifunctional peoriae paenibacillus and application thereof, and relates to the technical field of microorganisms, and the key point of the technical scheme is as follows: Paenibacillus peoriae S6330 is separated from rhizosphere soil; the peoriae paenibacillus has a good inhibition effect on pathogenic bacteria, pathogenic fungi and oomycetes; meanwhile, the peoriae paenibacillus has the functions of dissolving potassium, producing protease, producing amylase, producing cellulase, producing siderophore and the like, has a growth promoting effect on various crops such as wheat, corn, watermelons, small oilseed rapes, tobaccos and the like, and can remarkably improve indexes such as chlorophyll content, plant height, fresh weight, overground part weight, underground part weight and the like of plants; in addition, the peoriae paenibacillus can be used for effectively inhibiting the tobacco bacterial wilt; the peoriae paenibacillus has no environmental pollution problem, is simple in culture condition, and has wide disease prevention and growth promotion application prospects in agricultural production.

The invention discloses Delftia tsuhuensis and an application of the Delftia tsuhuensis in prevention and treatment of plant diseases. The Delftia tsuhuensis CDJ2 is preserved in the Guangdong Microbial Culture Collection Center on October 18, 2024, and the preservation number is GDMCC (China General Microbiological Culture Collection Center) NO: 65290. The strain has a good prevention and treatment effect on citrus canker, and the prevention and treatment effect can reach 84%. Meanwhile, the strain can also effectively prevent and treat soft rot of taro, bacterial basal rot of rice, bacterial wilt of crops, kiwifruit canker, angular leaf spot of mango, black rot of brassicaceous vegetables, tomato scab, gummosis of sugarcane, bacterial wilt of walnuts and bacterial leaf spot of plums. Wide application prospects are realized in the aspects of preventing and treating diseases of crops and commercial crops and promoting healthy growth of plants.

The invention belongs to the technical field of genetic transformation, and particularly relates to application of tomato Halide linker dissociation enzyme, a method for improving crop bacterial wilt resistance and a method for inhibiting pathogenic bacteria through protein. The invention provides application of a tomato Halide linker dissociation enzyme. The amino acid sequence of the tomato Halide linker dissociation enzyme SlMOC1 is as shown in SEQ ID NO: 2. The nucleotide sequence of the coding gene SlMOC1 is as shown in SEQ ID NO: 1. The result of the embodiment shows that the bacterial wilt resistance of the plant can be improved by overexpression of the tomato Halide linker dissociation enzyme SlMOC1 gene.

The invention provides a microbial agent type bio-organic fertilizer capable of improving continuous cropping tobacco planting soil hardening and a preparation method of the microbial agent type bio-organic fertilizer, and belongs to the technical field of organic fertilizers. The microbial agent type bio-organic fertilizer capable of improving continuous cropping tobacco planting soil hardening comprises the following raw materials: an organic material, a microbial agent, an inorganic nutritional ingredient, vinasse and an additive, wherein the organic material is composed of livestock and poultry manure, crop straw, tobacco waste and a fermentation inoculant; the additive is prepared from humic acid, seaweed extract and amino acid. The organic fertilizer contains rich organic materials and humic acid, the content of organic matters in soil can be increased, the granular structure of the soil can be improved, the growth of soil-borne pathogenic bacteria can be inhibited by the added multiple beneficial microorganisms, the micro-ecological environment of the soil can be improved by the photosynthetic bacteria, and the incidence rate of diseases such as tobacco black shank and bacterial wilt can be reduced.

The invention provides a ginger planting method for preventing and treating ginger bacterial wilt, and belongs to the technical field of ginger planting.According to the ginger planting method, through crop rotation, a ginger bacterial wilt pathogenic bacterium host chain is effectively cut off, ralstonia solanacearum is inhibited, harmful pathogenic bacteria in soil are removed through sun exposure, a biochar-probiotic mixture is applied, and meanwhile the harmful pathogenic bacteria in the soil are inhibited; biochar is utilized to increase porosity and water-holding capacity, relieve the problem of soil hardening, increase the pH value of soil, increase the content of alkaline cations, effectively inhibit growth of pathogenic bacteria of ginger blast, and also adsorb and retain nutrients such as ammonium and potassium through static electricity, and the pore structure of the biochar provides a shelter for probiotic groups, so that the probiotic groups are planted in the soil, and the soil quality is improved. The living space of the ginger blast pathogenic bacteria is inhibited; seed gingers are soaked in bacterium liquid containing bacteriophages, ginger blast pathogenic bacteria are effectively inhibited, drip irrigation is adopted for later-period management to maintain soil humidity, reproduction and spreading of the ginger blast pathogenic bacteria are effectively prevented and controlled, charcoal base fertilizer is additionally applied, and finally ginger blast is effectively prevented and controlled.

The invention discloses a crop ralstonia solanacearum disease prediction method based on an integrated learning model, and the method comprises the following steps: S1, collecting a published 16s rRNA gene sequence related to solanaceae crop bacterial wilt, and carrying out the preprocessing of original sequencing data based on an EasyAmplicon standardized process; s2, performing data dimension reduction by using a principal component analysis algorithm, and retaining 95% of variance; s3, performing hyper-parameter search based on 5-fold cross validation and grid search on the Light GBM model, the CatBoost model and the XGBoost model respectively, and selecting three groups of optimal hyper-parameters of each model; s4, constructing a model according to three groups of optimal hyper-parameters of each selected model, performing prediction, analyzing model result difference based on a Pearson correlation coefficient, and retaining Pearson correlation coefficient mean < lt > with other eight model prediction values; a model of 0.8; and S5, inputting the screened model prediction result into a second-layer element learner RF for integrated learning to obtain a final prediction result.

The invention discloses bacillus velezensis BR-1 and application of the bacillus velezensis BR-1 in prevention and treatment of economic crop diseases. The preservation number of the bacillus velezensis BR-1 is CCTCC NO: M 20251379. The bacillus velezensis BR-1 disclosed by the invention is high in adaptability and wide in growth condition range, and is more beneficial to application in agricultural production. Meanwhile, the bacillus velezensis BR-1 has a remarkable inhibition effect on the growth of various phytopathogens such as verticillium dahliae, fusarium oxysporum, sclerotinia sclerotiorum and ralstonia solanacearum, can remarkably reduce the morbidity of cotton verticillium wilt, fusarium wilt and tomato bacterial wilt under a greenhouse condition, and has a wide agricultural application prospect.

The invention discloses an application of an NtWRKY59 gene in regulation and control of tobacco bacterial wilt resistance. A nucleotide sequence of the NtWRKY59 gene is shown as SEQ ID NO.1, and the NtWRKY59 gene is over-expressed in tobacco. The invention further discloses an application of the NtWRKY59 gene in regulation and control of tobacco bacterial wilt resistance. The NtWRKY59 gene provided by the invention not only enriches the genetic basis of tobacco bacterial wilt resistance as a brand new anti-disease gene resource, but also provides a new thought for developing a disease-resistant breeding strategy different from an upstream signal element as an action mechanism of a downstream transcription factor, and has good scientific research value and application prospect.

Bacteriophages that comprise a tail fiber protein sequence comprising a C terminus host recognition site described by SEQ ID NO: 1 and which infect Ralstonia solanacearum which causes bacterial wilt disease are disclosed. Compositions comprising these bacteriophages may be employed as wilt control agents. Methods for preventing or treating diseases caused by Ralstonia solanacearum such as bacterial wilt disease are also disclosed. A bacterial wilt disease control method in a plant may include administering such bacteriophages to a plant or to a plant growth medium and can be effective against a wide variety of Ralstonia solanacearum strains. Nucleic acids encoding the C terminus host recognition site and amino acid sequences comprising the host recognition site are also disclosed.

The invention discloses a microbial agent for preventing and treating tomato bacterial wilt and a prevention and treatment method, and belongs to the technical field of plant disease prevention and treatment. According to the microbial agent for preventing and treating tomato bacterial wilt and the prevention and treatment method, the morbidity of the bacterial wilt can be remarkably reduced, and the microbial agent has a good application prospect in agricultural production. Compared with chemical pesticides, the biological prevention and control method provided by the invention has small influence on the environment and is a more environment-friendly prevention and control means.

The invention provides a carbon-based bio-fertilizer, a preparation method thereof and application of the carbon-based bio-fertilizer in prevention and treatment of tomato bacterial wilt, and belongs to the technical field of biological prevention and treatment of plant diseases. The carbon-based bio-fertilizer for preventing and treating tomato bacterial wilt is prepared from the following raw materials in parts by weight: 22 to 28 parts of livestock manure, 10 to 15 parts of straw, 10 to 15 parts of mushroom dregs, 10 to 15 parts of charcoal, 6 to 9 parts of medicine dregs, 8 to 12 parts of potassium humate, 6 to 8 parts of pine sawdust, 3 to 6 parts of microbial agent and 4 to 6 parts of attapulgite powder. The microbial agent comprises paecilomyces lilacinus, streptomyces microflavus, bacillus megatherium, lactobacillus plantarum and photosynthetic bacteria. The carbon-based bio-fertilizer for preventing and treating tomato bacterial wilt is composed of different organic wastes and specific microbial flora, is not easy to generate drug resistance, can quickly form dominant flora after being applied to soil, and can effectively antagonize tomato bacterial wilt pathogenic bacteria and prevent and treat tomato bacterial wilt.

The invention relates to the field of microbial prevention and control, in particular to a Riebsiella spp. SHP-47 and application thereof. The invention provides a strain of Kitasatosporidium sp. SHP-47, and the preservation number of the Kitasatosporidium sp. SHP-47 is CGMCC (China General Microbiological Culture Collection Center) No.33249. The invention also provides a method for preparing the Kitasatosporidium sp. SHP-47. The strain has a remarkable inhibition effect on pathogenic bacteria of eucalyptus bacterial wilt, can effectively reduce occurrence and spreading of diseases, and provides a new way for green prevention and treatment of eucalyptus; meanwhile, the problems that in the prior art, eucalyptus bacterial wilt prevention and control means are insufficient, and use of chemical pesticides brings negative effects to the environment are solved.

The invention relates to the technical field of microbial pesticides and microbial fertilizers, in particular to actinomycetes antagonizing various plant pathogens and application of the actinomycetes antagonizing various plant pathogens, and provides a YC3041 strain with the taxonomic name of Streptomyces pratensis and the preservation number of GDMCC No: 64828. The technical scheme provided by the invention has the characteristics of low use cost, good control effect, no residue and safety to human, livestock and environment, and the actinomycetes antagonizing various plant pathogens and the application of the actinomycetes antagonizing various plant pathogens have broad application prospects. The biological pesticide prepared from the YC3041 strain has the control effect of 70% or above on tobacco root knot nematode disease, tobacco bacterial wilt, tobacco black shank and compound diseases caused by the tobacco root knot nematode disease, the tobacco bacterial wilt and the tobacco black shank.

The invention belongs to the technical field of biocontrol bacteria, and discloses bacillus velezensis and application thereof in preventing and treating bacterial wilt of solanaceae crops. The bacillus velezensis is named as bacillus velezensis PR-7, and is preserved in the China General Microbiological Culture Collection Center (CGMCC), the address of the preservation unit is No.3, No.1 yard, Beichen West Road, Chaoyang District, Beijing, the preservation time is November 13, 2023, and the preservation number is CGMCC No.28979. The bacillus velezensis has the advantages that the bacillus velezensis is named as bacillus velezensis PR-7; the bacillus velezensis can effectively inhibit the growth of a bacterial wilt pathogen, i.e., the evolutionary I type (main evolutionary type of bacterial wilt pathogen in South China) of bacterial wilt pathogen, improves the bacterial wilt resistance of solanaceae crops, and is of great significance to the prevention and treatment of bacterial wilt of solanaceae crops in South China.

The invention discloses application of cobalamin and beneficial bacteria in prevention and control of tomato soil-borne bacterial wilt. The beneficial bacterium is Chryseobacterium CHR6, and the preservation number of the strain is CGMCC (China General Microbiological Culture Collection Center) The invention discloses a synbiotics for preventing and controlling tomato soil-borne bacterial wilt. The synbiotics consist of beneficial bacteria and cobalamin, and the beneficial bacterium is Chryseobacterium CHR6. The invention discloses a method for preventing and controlling tomato soil-borne bacterial wilt by matching cobalamin and beneficial bacteria, which comprises the following steps: 7-10 days after transplanting tomato seedlings, adding synbiotics into soil by a root irrigation method, adding 10-20 mL of cobalamin solution with the concentration of 5-10 nM into each tomato seedling, and inoculating 10-20 mL of beneficial bacteria suspension with the concentration of 1 * 10 < 7 >-1 * 10 < 9 > CFU / mL. By adding cobalamin, inhibition of growth of ralstonia solanacearum through siderophore mediation of Chryseobacterium CHR6 can be remarkably enhanced, and occurrence of tomato soil-borne bacterial wilt is reduced.

The invention relates to a ralstonia solanacearum bacteriophage LC10 capable of preventing and controlling tobacco bacterial wilt, the classification name of the ralstonia solanacearum bacteriophage LC10 is Rastonia phage, the preservation number of the ralstonia solanacearum bacteriophage LC10 is GDMCC No: 66716-B1, and the preservation date of the ralstonia solanacearum bacteriophage LC10 is July 21, 2025. The bacteriophage is a brachytail bacteriophage and can specifically split ralstonia solanacearum, inhibit the growth of the ralstonia solanacearum and enhance the disease inhibition capability of soil. Therefore, the bacteriophage LC10 can be used for preparing related preparations for preventing and controlling tobacco bacterial wilt.

The Bacillus velezensis M173 was deposited with the Guangdong Microbial Culture Collection Center with a deposit number of GDMCC No. 61434. After being prepared into a seed-coating agent, Bacillus velezensis M173 can promote the growth and germination of plants and significantly improve the control effect on pathogens. Moreover, Bacillus velezensis M173 can relieve the condition of dead seedlings of crops at seedling stage after being applied to the crops. In particular, both pot experiments and field experiments prove that the Bacillus velezensis M173 of the present invention has a significant control effect on fungal and bacterial diseases, in particular, bacterial wilt and stem rot. Therefore, the Bacillus velezensis M173 of the present invention has broad application prospects.

The invention discloses an ecological prevention and control method for tomato bacterial wilt based on a multi-nutritional-level food web and application of the ecological prevention and control method, and belongs to the technical field of crop disease prevention and control. According to the ecological prevention and control method for tomato bacterial wilt provided by the invention, indigenous dominant heterophagous nematodes and bacterial-feeding nematodes which are widely distributed in soil are introduced, so that the structure of a food web of the soil is adjusted, and the natural inhibition effect of tomatoes on the bacterial wilt is enhanced; the ecological balance of soil is improved, the activity of beneficial microorganisms is increased, field planting of ralstonia solanacearum at rhizosphere is inhibited, and the plant morbidity is remarkably reduced; further, healthy growth and biomass accumulation of the tomatoes are facilitated, and the plant height and the dry weight of the overground part of the plants are remarkably increased; the provided control method is simple and convenient to operate, wide in application range and easy to implement, does not depend on chemicals, reduces environmental pollution and potential food safety hazards, and has wide popularization value.

The invention belongs to the technical field of microorganisms. The invention relates to a coxakella cocori Kos-10 strain, in particular to a coxakella cocori Kos-10 strain and application thereof. The invention discloses a Cosakya cokei Kos-10 strain which is preserved in the Guangdong Microbial Culture Collection Center on June 5, 2025, and the preservation number of the Cosakya cokei Kos-10 strain is GDMCC (China General Microbiological Culture Collection Center) NO: 66468. The Cosakya cokei Kos-10 strain has been preserved in the Guangdong Microbial Culture Collection Center on June 5, 2025. The strain has the capabilities of fixing nitrogen, dissolving phosphorus, dissolving potassium and producing siderophores, can be used as a growth-promoting bacterium for promoting plant growth, provides nutrient elements of nitrogen, potassium and iron for plants, and also can prevent and treat bacterial wilt of the plants. The Kos-10 strain disclosed by the invention is comprehensive in growth promoting effect, and has a good application prospect and application value in the aspect of promoting plant growth under the stress of bacterial wilt.

The invention discloses a compound reagent for preventing and treating tobacco bacterial wilt and application, and belongs to the technical field of tobacco disease prevention and control, the compound reagent is prepared by taking epsilon-polylysine and biocontrol bacterium fermentation liquor according to the mass ratio of (1-2): (3-5), then the compound reagent is sprayed on tobacco leaves, the cigar bacterial wilt can be effectively prevented and treated, and the yield of cigars is increased. By adopting the biological agent, the tobacco bacterial wilt is effectively prevented and treated, meanwhile, pathogenic bacteria are prevented from generating drug resistance, and meanwhile damage to the environment is relieved.

The invention relates to the technical field of microorganisms, in particular to a ginger bacterial wilt biocontrol strain caused by enterobacter cloacae and a ginger bacterial wilt control method. The name of the ginger bacterial wilt biocontrol strain is bacillus cereus Y51, the strain is preserved in the China General Microbiological Culture Collection Center on May 9, 2024, and the preservation number of the strain is CGMCC NO.30536. The invention further discloses a preparation method of the ginger bacterial wilt biocontrol strain. Experiments prove that the bacillus cereus Y51 has a good prevention effect on the enterobacter cloacae on ginger plants, so that the bacillus cereus Y51 can be used for preventing and treating the ginger blast, and has the advantages of being environmentally friendly, free of pesticide residues and green and environmentally friendly. The biocontrol strain provided by the invention fills up the blank of the existing ginger bacterial wilt biological control strain in the aspect of targeting specific pathogenic bacteria species.

The invention discloses an application of an NtWRKY41 gene in regulation and control of tobacco bacterial wilt resistance. A nucleotide sequence of the NtWRKY41 gene is shown as SEQ ID NO.1, and the NtWRKY41 gene is over-expressed in tobacco. The invention further discloses an application of the NtWRKY41 gene in regulation and control of tobacco bacterial wilt resistance. The NtWRKY41 gene provided by the invention not only enriches the genetic basis of tobacco bacterial wilt resistance as a brand new anti-disease gene resource, but also provides a new thought for developing a disease-resistant breeding strategy different from an upstream signal element as an action mechanism of a downstream transcription factor, and has good scientific research value and application prospect.

The invention provides a method for improving the disease resistance of plants, and particularly provides a method for improving the disease resistance of the plants by reducing or inhibiting the expression quantity and / or activity of SWEET11a genes or encoded proteins thereof. According to the method, the disease resistance of the plants can be improved, particularly the resistance to gray speck disease, northern leaf blight, bacterial wilt and stem rot is improved, and the method has important application value in biological breeding.

The invention discloses enterobacter hormaechei and application thereof, and belongs to the technical field of microorganisms. The strain is named as Enterobacter hormaechei (Enterobacter hormaechei) zhegui 61401, is preserved in the China Center for Type Culture Collection (CCTCC), and has the preservation number of CCTCC NO: M 20241827. The enterobacter hormaechei provided by the invention has the effect of relieving the influence of acid stress on plants, can inhibit the growth of ralstonia solanacearum, can be applied to biological prevention and control of plant bacterial wilt in an acidified soil area, and expands the application range of the enterobacter hormaechei. The invention provides a feasible solution for sustainable production of tomatoes in the acidified soil area.