123 results about "Bacterial wilt" patented technology

The invention discloses an application of an NtWRKY26 gene in regulation and control of tobacco bacterial wilt resistance. A nucleotide sequence of the NtWRKY26 gene is shown as SEQ ID NO.1, and the NtWRKY26 gene is over-expressed in tobacco. The invention also discloses an application of the NtWRKY26 gene in regulation and control of tobacco bacterial wilt resistance. The NtWRKY26 gene provided by the invention not only enriches the genetic basis of tobacco bacterial wilt resistance as a brand new anti-disease gene resource, but also provides a new thought for developing a disease-resistant breeding strategy different from an upstream signal element as an action mechanism of a downstream transcription factor, and has good scientific research value and application prospect.

The invention relates to the technical field of plant genetic engineering, in particular to a peanut bacterial wilt resistance related gene Ah6Q1KS5, an encoding protein thereof, a biological material and application of the gene Ah6Q1KS5 in disease-resistant breeding. Through whole genome identification and functional analysis, the gene Ah6Q1KS5 which is remarkably related to bacterial wilt resistance in a peanut SAMS gene family is screened, and the gene is induced by ralstonia solanacearum, salicylic acid (SA), abscisic acid (ABA) and methyl jasmonate (MeJA) to be remarkably expressed in a peanut bacterial wilt resistant variety H108. Peanut and tobacco plants over-expressing Ah6Q1KS5 can significantly reduce cell death, inhibit growth of ralstonia solanacearum, and enhance resistance to bacterial wilt. The molecular marker can be used for analyzing the molecular mechanism of peanut bacterial wilt resistance, provides new gene resources and technical means for cultivating bacterial wilt resistant peanut varieties, and has important application value in the field of peanut breeding.

The application discloses a staphylococcus and application of the staphylococcus in preparation of a control agent for tobacco black shank and tobacco bacterial wilt, relates to the technical field of microorganisms, and the staphylococcus 3C obtained by the application has obvious inhibiting effect on tobacco phytophthora and ralstonia solanacearum, and the fermentation liquor prepared from the staphylococcus 3C has obvious prevention and treatment effect on tobacco black shank and tobacco bacterial wilt.

The present application relates to a strain of Streptomyces griseoaurantiacus and its application, belonging to the technical field of biological control of plant diseases. The Streptomyces griseoaurantiacus of the present application has been preserved in Guangdong Microbial Culture Collection Center on July 28, 2023, with the preservation number of GDMCC No: 63691 and the preservation address of No. 59 Building, 5th Floor, 100, Martyrs' Road, Guangzhou, Guangdong Province. The strain can inhibit the formation of Ralstonia solanacearum biofilm and the occurrence of Ralstonia solanacearum caused by bacterial wilt, has good prevention and control effect on bacterial wilt, and can be used as a biocontrol agent and biological preparation for the prevention and control of bacterial wilt.

The application discloses a preparation method of a nanogel preparation for preventing and treating tobacco diseases, and specifically comprises the following steps: (1) preparing bacillus licheniformis bacterial liquid; (2) preparing nanometer selenium precipitate; (3) preparing purified nanometer selenium particles; (4) preparing streptomycin-nanometer selenium solid; (5) preparing nanometer titanium dioxide photo-semiconductor sol; and (6) mixing low-melting agarose powder, the streptomycin-nanometer selenium solid and the nanometer titanium dioxide photo-semiconductor sol, centrifuging, washing, and drying to obtain the nanogel preparation. The nanogel preparation has significant bacteriostatic efficacy, can effectively prevent and treat tobacco diseases (especially tobacco bacterial wilt and tobacco wild fire disease), has a promoting effect on tobacco growth, is simple to make, has strong adhesion, and does not pollute the soil environment.

The invention belongs to the technical field of molecular biology, and discloses a molecular marker remarkably related to cassava bacterial wilt resistance and application of the molecular marker. The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker related to cassava bacterial wilt resistance, which is characterized in that the sequence of the SNP molecular marker is shown as SEQ ID NO: 3, the SNP site is positioned at the 392nd site from the 5'terminal of the sequence shown as SEQ ID NO: 3, and the polymorphism of the SNP molecular marker is A / T. By detecting the genotype of the SNP molecular marker, cassava with strong resistance to bacterial wilt can be accurately selected, and the SNP molecular marker can be effectively used for cassava molecular marker assisted breeding; according to actual breeding requirements, genotype identification can be carried out on cassava, genotype cassava with high bacterial wilt resistance is selected for hybridization, cassava filial generation with high bacterial wilt resistance is obtained, breeding time is saved, cost is low, accuracy is high, and the cassava breeding process is accelerated.

The invention relates to bacillus safensis AHAU-WU410 and application of the bacillus safensis AHAU-WU410 in prevention and treatment of corn bacterial wilt and promotion of corn plant growth, and belongs to the technical field of biological prevention and treatment, a living body pure culture of the bacillus safensis AHAU-WU410 is preserved in China Center for Type Culture Collection in Wuhan University, the preservation date is August 4, 2025, the preservation number is CCTCC NO: M 20251769, and the preservation number is CCTCC NO: M 20251769. The bacillus safensis AHAU-WU410 has a remarkable antagonistic effect on pathogenic bacteria pantoea stutzeri of the corn bacterial wilt, and can promote the growth of corn plants by secreting IAA (Indoleacetic Acid) and playing a phosphate solubilizing role. The bacillus safensis AHAU-WU410 is screened and identified, the control effect, the biocontrol mechanism and the growth promoting function of the biocontrol bacterium are verified and analyzed, and a new direction and a theoretical basis are provided for developing a green biocontrol bacterium agent related to corn bacterial disease control.

The application provides bacillus velezensis HNC-17 and an application thereof, and belongs to the technical field of microorganisms. Bacillus velezensis The bacillus velezensis (HNC-17) of the application is preserved in the Guangdong Microbial Digital Culture Collection Center, and the preservation number is GDMCC No:68053. Ralstonia solanacearum The bacillus velezensis HNC-17 with antagonistic effect on ralstonia solanacearum is screened from the rhizosphere soil of cigar tobacco, and the optimal culture medium component and fermentation condition of the strain are optimized and determined. The prevention and treatment effect of the bacillus velezensis HNC-17 of the application on the cigar tobacco bacterial wilt reaches 74.59%, and the growth of cigar tobacco seedlings can be significantly promoted, which lays a foundation for the development of a bacterial agent for the bacterial wilt of cigar tobacco and the green prevention and control of the bacterial wilt.

The invention provides a bacillus velezensis QT1 and an application of the bacillus velezensis QT1. The classification name of the bacillus velezensis is bacillus velezensis QT1, and the bacillus velezensis is preserved in the China Center for Type Culture Collection on November 12, 2025, the preservation address is Wuhan University, Wuhan, China, and the preservation number is CCTCC NO: M 20252531. The strain shows remarkable biological control efficiency, realizes effective control on various plant pathogens, and can prevent and treat tomato bacterial wilt and tea tree anthracnose. In addition, the bacillus velezensis QT1 can also effectively kill root-knot nematodes (especially meloidogyne incognita). When the strain is applied to plant cultivation, the growth of various pathogens can be remarkably inhibited, the health and yield of crops are improved, the strain is environment-friendly, the use of chemical pesticides is reduced, soil pollution is avoided, and the development requirement of sustainable agriculture is met.

The present application relates to the technical field of plant breeding, in particular to StERF1A Application of a gene related biological agent in improving the resistance of potato to bacterial wilt, the biological agent StERF1A The nucleotide sequence of the gene is shown as SEQ ID NO. 1. The present application discloses for the first time StERF1A As a negative regulatory factor involved in the mechanism of potato resistance to bacterial wilt: overexpression StERF1A Can increase the accumulation amount of bacterial wilt in leaves, inhibit the expression of disease resistance related genes Pto 、 PR1b1 And PR1a The content of active oxygen ROS and the activity of related enzymes SOD, POD and CAT are reduced; and the expression of down-regulation StERF1A The opposite effect is presented, which significantly enhances the resistance of potato to bacterial wilt. The present application provides an important gene target and theoretical basis for breeding new potato varieties with high resistance to bacterial wilt.

The Bacillus velezensis M173 was deposited with the Guangdong Microbial Culture Collection Center with a deposit number of GDMCC No. 61434. After being prepared into a seed-coating agent, Bacillus velezensis M173 can promote the growth and germination of plants and significantly improve the control effect on pathogens. Moreover, Bacillus velezensis M173 can relieve the condition of dead seedlings of crops at seedling stage after being applied to the crops. In particular, both pot experiments and field experiments prove that the Bacillus velezensis M173 of the present invention has a significant control effect on fungal and bacterial diseases, in particular, bacterial wilt and stem rot. Therefore, the Bacillus velezensis M173 of the present invention has broad application prospects.

The invention provides a peanut bacterial wilt resistance NBS-LRR coding gene AhRRS3 and application thereof. The nucleotide sequence of the gene AhRRS3 is shown as SEQ ID NO.1, and the amino acid sequence of the protein coded by the gene AhRRS3 is shown as SEQ ID NO.2. The invention further provides a preparation method of the peanut bacterial wilt resistance NBS-LRR coding gene AhRRS3. According to the invention, an overexpression vector of AhRRS3 is constructed, arabidopsis thaliana is transformed, and the disease-resistant function of the arabidopsis thaliana is observed. Research results show that after being infected by ralstonia solanacearum, the disease-resistant variety of arabidopsis thaliana and a disease-susceptible strain of the overexpressed gene AhRRS3 show obvious disease resistance, the disease-susceptible strain is in a whole-plant withering and death state, and the results show that the overexpressed gene AhRRS3 can improve the bacterial wilt resistance of the plant. The invention provides high-quality gene resources and theoretical basis support for genetic engineering breeding of plant bacterial wilt resistance.

The invention relates to the technical field of microorganisms, in particular to bacillus aryabhattai OF326 and application of the bacillus aryabhattai OF326 to preparation of bio-organic fertilizer and disease resistance, the bacillus aryabhattai OF326 is a probiotic screened by the applicant, research finds that the strain has the functions of IAA production, phosphorus solubilization and nitrogen fixation, growth of rice seedlings can be remarkably promoted, and the yield of the rice seedlings is increased. The method is suitable for secondary fermentation of organic fertilizers with sheep manure and traditional Chinese medicine residues as raw materials, and the viable count of beneficial bacteria is increased. In addition, tests show that the strain OF326 has a remarkable antagonistic effect on ralstonia solanacearum, and a clear antibacterial zone is formed; and by compounding with inositol or trehalose-6-phosphoric acid (T6P), the proliferation of the strain can be further promoted, and the growth rate of the strain is increased. In conclusion, the strain still keeps good growth-promoting and disease-resistant functions under the waterflooding condition, and is suitable for development of biological fertilizers and disease-resistant preparations.

The invention provides a tobacco and sweet potato relay intercropping field planting method and application thereof, and belongs to the technical field of agricultural planting. The planting method provided by the invention comprises the following steps: after tobacco is harvested, intensively burning diseased roots, diseased plants and weeds in a field, deeply ploughing soil in winter, carrying out soil drying and sterilization, applying an organic fertilizer or a special fertilizer for sweet potatoes by adopting a strip application mode, carrying out tobacco and sweet potato intercropping by adopting a single-side opposite planting mode, applying a bacillus siamensis XZ92 bacterial liquid and a bacillus velezensis XZ93 bacterial liquid to rhizosphere of the intercropped tobacco, and carrying out intercropping on the tobacco and the sweet potatoes by adopting a single-side opposite planting mode. And pouring methyl jasmonate liquid into the tobaccos, and supplementing trace elements in stages to finish planting. The comprehensive prevention and control system for the tobacco bacterial wilt is constructed by optimizing the relay intercropping space layout of tobacco and sweet potatoes, cooperatively applying strains with specific functions and methyl jasmonate and designing a staged nutrient supply scheme.

The application provides a Yunnan golden camellia root endophytic fungus and application thereof, and relates to the technical field of crop disease prevention and treatment. The Yunnan golden camellia root endophytic fungus is Lecanicillium attenuatum JHC098, which is preserved in the China Center for Type Culture Collection and has a preservation number of CCTCC NO: M 2025749. The liquid fermentation broth extract of the fungus can be applied to crop disease prevention and treatment. The application overcomes the defects of the prior art. The liquid fermentation broth extract of the Yunnan golden camellia root endophytic fungus Lecanicillium attenuatum JHC098 can effectively inhibit the growth of citrus canker and tobacco bacterial wilt pathogenic bacteria, so that the purpose of biological prevention is achieved.

The invention discloses bacillus velezensis as well as a complex microbial inoculant and application thereof. The strain number of the bacillus velezensis D-3A is D-3A, the bacillus velezensis D-3A is registered in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.32670. The bacillus velezensis D-3A is a bacillus velezensis strain. The complex microbial inoculant comprises the bacillus velezensis and the bacillus subtilis. The bacillus velezensis D-3A separated for the first time and the bacillus subtilis PP are mixed to obtain the biocontrol bacterium complex microbial inoculant, the biocontrol bacterium complex microbial inoculant has a good inhibition effect on pathogenic bacteria of tobacco bacterial wilt, and the control effect is stable and lasting. The complex microbial inoculant influences soil mainly by activating nutrients (such as dissolving phosphorus, dissolving potassium and fixing nitrogen) and promoting organic matter conversion, hydrolytic nitrogen, available phosphorus and quick-acting potassium are remarkably increased, and the pH value of the soil is reduced to a certain extent.

The invention provides a DSE chlamydospore water aqua as well as a preparation method and application thereof, and belongs to the technical field of microbial preparations, the DSE chlamydospore water aqua comprises chlamydospore from at least one dark partitioned endophytic fungus, and further comprises 5g / mL-10g / mL of preservative and 5g / mL-10g / mL of protective agent, and the content of the chlamydospore is greater than 2.5 * 10 < 8 > CFU / mL. The product property of the water aqua is improved by reasonably optimizing the dosage of DSE chlamydospore and auxiliary materials, the content of chlamydospore in the obtained water aqua finished product can reach 2.54 * 10 < 8 > CFU / mL, and all indexes meet the national standard; the conidium water aqua can be stored for more than 12 months at normal temperature, and the shelf life is prolonged by more than 9 months compared with a conventional conidium water aqua. The DSE chlamydospore aqueous solution can effectively prevent and treat tomato bacterial wilt and southern rice black-streaked dwarf disease, improve the growth vigor of tomato plants and rice plants, and achieve a prevention and treatment effect which is not inferior to or even superior to that of positive control trichoderma harzianum. The DSE chlamydospore aqueous solution provided by the invention is simple to prepare, controllable in cost and excellent in performance, and is suitable for large-scale popularization and application as a novel biopesticide product.

The invention provides a peanut bacterial wilt resistant gene AhNHL24 as well as an identification method and application thereof, and belongs to the technical field of plant genetic engineering. The peanut disease resistance related gene AhNHL24 is separated from peanuts, the DNA sequence of the peanut disease resistance related gene AhNHL24 is shown as SEQ ID NO: 1, and the amino acid sequence of protein coded by the gene AhNHL24 is shown as SEQ ID NO: 2. According to the preparation method of the peanut disease-resistant gene, the AhNHL24 gene is separated and identified through Illumina RNA sequence and comparative transcriptome analysis, and transcription mode analysis and plant expression analysis find that the AhNHL24 gene can improve the resistance of plants to ralstonia solanacearum, so that the invention determines that the peanut AhNHL24 gene can be used as an important gene for peanut bacterial wilt resistance breeding.

The invention discloses an application of coumarin substances in prevention and treatment of eucalyptus bacterial wilt. The coumarin substances are daphnetin and 6-methyl coumarin. Bacteriostatic experiment research results show that the daphnetin and the 6-methylcoumarin have an obvious inhibition effect on growth and proliferation of eucalyptus bacterial wilt bacteria when being used independently or used in combination. When the daphnetin is independently used, the effective concentration of the daphnetin is greater than or equal to 62.5 mu g / mL when the daphnetin has an obvious inhibition effect on eucalyptus bacterial wilt pathogenic bacteria; when the 6-methylcoumarin is singly used, the effective concentration of the 6-methylcoumarin is greater than or equal to 125 mu g / mL when the 6-methylcoumarin has an obvious inhibition effect on eucalyptus bacterial When the daphnetin and the 6-methylcoumarin are used in a combined manner, the effective concentration of the daphnetin and the effective concentration of the 6-methylcoumarin are both larger than or equal to 31.25 microgram / mL when the daphnetin and the 6-methylcoumarin show an obvious inhibition effect on eucalyptus bacterial wilt pathogenic bacteria, and therefore the daphnetin and the 6-methylcoumarin can serve as bacteriostatic agents to be used for preventing and treating eucalyptus bacterial wilt diseases and have potential application prospects of biocontrol preparations.

The invention belongs to the technical field of complex microbial inoculant processing, and discloses a processing device of a complex microbial inoculant for preventing and treating bacterial wilt, the processing device comprises a lower tank, the top of the lower tank is fixedly communicated with an upper tank, the top of the upper tank is fixedly communicated with a plurality of feed pipes, and the center of the bottom of the lower tank is fixedly connected with a servo motor; through cooperation of structures such as a feeding pipe, a push disc and a feeding box, the situation that the concentration of local fungus liquid is too high during feeding is avoided, when the feeding box moves downwards, the space formed by the push disc and an inner cavity of the feeding box is compressed and reduced, the pressure intensity is increased, a first one-way valve is opened through the pressure intensity to communicate with a vertical through groove, and the feeding effect is improved. At the moment, fungus liquid in the inner cavity of the feeding box can enter the vertical through groove along the arc-shaped concave surface of the push disc and is finally injected into the bacterial liquid in the lower tank through the feeding nozzle, the multiple pairs of feeding pipes are arranged, the feeding points are increased, the feeding amount is dispersed, the local fungus liquid is prevented from being too high in concentration, the stirring difficulty is reduced, the stirring time is shortened, and therefore the stability of the co-growth environment of strains is guaranteed.

The invention discloses bacillus siamensis and application thereof, and relates to the technical field of microorganisms. The strain is bacillus siamensis LYQ15 which is preserved in the China General Microbiological Culture Collection Center (CGMCC) on March 14, 2024, the preservation address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC NO: 30024. The strain provided by the invention can be used for effectively inhibiting the growth and reproduction of phytophthora and pseudomonas solanacearum as well as related diseases caused by the growth and reproduction of the phytophthora and the pseudomonas solanacearum; in addition, the bacillus siamensis LYQ15 can inhibit reproduction of pathogenic bacteria and epidemic of diseases in an environment with the pathogenic bacteria, and healthy growth of tobacco plants is guaranteed.

This invention belongs to the field of fermentation engineering and plant disease control technology, specifically relating to the application of a Bacillus endophyticus compound inoculant fermentation broth in the control of plant diseases. The Bacillus endophyticus compound inoculant disclosed in this invention exhibits antibacterial effects against anthracnose in peppers, blight in peppers, gray mold in tomatoes, wilt in watermelons, wilt in cucumbers, verticillium wilt in cotton, black spot in peppers, root rot in carrots, soft rot in taro, and bacterial wilt in peppers, with inhibition rates of (75.37±1.44), (71.00±1.41)%, (83.14±0.96)%, (74.70±1.09)%, (69.21±1.04)%, (78.31±1.39)%, (73.06±0.73)%, (75.49±0.89)%, (46.79±1.57)%, and (73.82±0.51)%, respectively.

The invention discloses application of limonin in preventing and treating bacterial wilt of cigars. According to the method, limonin is applied by adopting a foliar spraying method, so that the relative control effect of cigars on bacterial wilt can be remarkably improved, and the morbidity of the bacterial wilt of cigars is reduced.

The invention discloses a strain of Chryseobacterium wanjuense C14, and a preparation method of the Chryseobacterium wanjuense C14. The invention further discloses application of the bacillus subtilis in inhibition of ralstonia solanacearum and application of the bacillus subtilis in degradation of straw. The Chryseobacterium sp. C14 can obviously inhibit the growth of pathogenic bacteria of tomato bacterial wilt, especially under the assistance of tomato rhizosphere metabolites such as O-phosphoethanolamine, mangiferin and L-tryptophan, the antibacterial effect of the Chryseobacterium sp. C14 is further enhanced, and the Chryseobacterium sp. C14 has higher environmental adaptability and prevention and treatment potential. The Chryseobacterium sp. C14 can also degrade protein, cellulose and hemicellulose at the same time, and can significantly promote the degradation of rice straw and corn straw. The Chryseobacterium sp. C14 has the functions of straw degradation and tomato ralstonia solanacearum inhibition, integrates a soil-borne disease biological control function and efficient straw degradation, and provides a green and efficient microbial new strategy for solving the problems of difficult straw treatment and difficult tomato bacterial wilt control in agricultural production.

The invention discloses a biopesticide composition for preventing and treating sesame bacterial wilt and a preparation, and belongs to the technical field of plant protection. The biopesticide composition for preventing and treating sesame bacterial wilt comprises the following raw materials in parts by mass: 30-40 parts of 50% carbendazim wettable powder, 2-3 parts of spinach / chamomile mixed extract, 2.5-3.5 parts of citrus peel extract and 1.5-2 parts of compound microorganisms. The biopesticide composition for preventing and treating sesame bacterial wilt has a good bacteriostatic effect on pathogenic bacteria, can effectively inhibit infection of the pathogenic bacteria on plants, can reduce the dosage of chemical agents, can reduce the risk that the pathogenic bacteria generate drug resistance, can effectively prevent and relieve the disease condition of sesame, and has a good application prospect. The influence of bacterial wilt on the economic crop yield is reduced.

The application discloses a preparation method and application of a novel nano-antibiotic agent for preventing and treating tobacco bacterial wilt, and belongs to the technical field of tobacco bacterial wilt prevention and treatment. Under the catalysis of N, N'-dicyclohexyl carbodiimide and 4-dimethylaminopyridine, daphnetin is condensed with carboxymethyl chitosan to form nanoparticles, and the prepared novel nanoparticles have high water solubility and good prevention and treatment effect.

The application discloses a kind of complex microbial inoculant for resisting tomato bacterial wilt and a preparation method thereof, and belongs to the technical field of microbial materials.The inoculant comprises functional microorganisms, microbial stimulants, soluble starch, xanthan gum and water.A biocontrol fungus, Bacillus subtilis, which is highly efficient in antagonizing tomato bacterial wilt, is screened.The fungus has a strong inhibitory effect on pathogenic bacteria and can promote tomato growth, and can form a biofilm and colonize the root system efficiently.The use of the fungus in combination with microbial stimulants can effectively improve the activity of the strain, and the strain can continuously play a role in the root system of the plant, and the biocontrol effect is remarkable.Finally, using soluble starch and xanthan gum as carriers, the raw materials jointly act to achieve long-term prevention and treatment of tomato bacterial wilt, and significantly improve the yield and quality of tomatoes.

This invention belongs to the field of biotechnology. To improve the effectiveness of biological control of plant diseases, this invention discloses *Bacillus velezensis* ZF516 and its application in controlling various soil-borne diseases. The *Bacillus velezensis* strain number is ZF516, and its registration number at the China General Microbiological Culture Collection Center is CGMCC No. 30136. This *Bacillus velezensis* strain can prevent and / or treat tomato bacterial canker, tomato bacterial wilt, or cowpea root rot, or can be used to prepare products for the prevention and / or treatment of these diseases. This strain also exhibits good inhibitory effects against *Fusarium oxysporum*, *Diplostomum spp.*, *Rhizoctonia solani*, *Fusarium solanum*, *Corynebacterium multiflorum*, *Botrytis cinerea*, *Agrobacterium tumefaciens*, *Pectinobacter brasiliensis*, *Ralstonia solanacearum*, *Streptomyces spp.*, etc.