41 results about "Bacterial wilt" patented technology

The application discloses a staphylococcus and application of the staphylococcus in preparation of a control agent for tobacco black shank and tobacco bacterial wilt, relates to the technical field of microorganisms, and the staphylococcus 3C obtained by the application has obvious inhibiting effect on tobacco phytophthora and ralstonia solanacearum, and the fermentation liquor prepared from the staphylococcus 3C has obvious prevention and treatment effect on tobacco black shank and tobacco bacterial wilt.

The application provides bacillus velezensis HNC-17 and an application thereof, and belongs to the technical field of microorganisms. Bacillus velezensis The bacillus velezensis (HNC-17) of the application is preserved in the Guangdong Microbial Digital Culture Collection Center, and the preservation number is GDMCC No:68053. Ralstonia solanacearum The bacillus velezensis HNC-17 with antagonistic effect on ralstonia solanacearum is screened from the rhizosphere soil of cigar tobacco, and the optimal culture medium component and fermentation condition of the strain are optimized and determined. The prevention and treatment effect of the bacillus velezensis HNC-17 of the application on the cigar tobacco bacterial wilt reaches 74.59%, and the growth of cigar tobacco seedlings can be significantly promoted, which lays a foundation for the development of a bacterial agent for the bacterial wilt of cigar tobacco and the green prevention and control of the bacterial wilt.

The invention provides a bacillus velezensis QT1 and an application of the bacillus velezensis QT1. The classification name of the bacillus velezensis is bacillus velezensis QT1, and the bacillus velezensis is preserved in the China Center for Type Culture Collection on November 12, 2025, the preservation address is Wuhan University, Wuhan, China, and the preservation number is CCTCC NO: M 20252531. The strain shows remarkable biological control efficiency, realizes effective control on various plant pathogens, and can prevent and treat tomato bacterial wilt and tea tree anthracnose. In addition, the bacillus velezensis QT1 can also effectively kill root-knot nematodes (especially meloidogyne incognita). When the strain is applied to plant cultivation, the growth of various pathogens can be remarkably inhibited, the health and yield of crops are improved, the strain is environment-friendly, the use of chemical pesticides is reduced, soil pollution is avoided, and the development requirement of sustainable agriculture is met.

The present application relates to the technical field of plant breeding, in particular to StERF1A Application of a gene related biological agent in improving the resistance of potato to bacterial wilt, the biological agent StERF1A The nucleotide sequence of the gene is shown as SEQ ID NO. 1. The present application discloses for the first time StERF1A As a negative regulatory factor involved in the mechanism of potato resistance to bacterial wilt: overexpression StERF1A Can increase the accumulation amount of bacterial wilt in leaves, inhibit the expression of disease resistance related genes Pto 、 PR1b1 And PR1a The content of active oxygen ROS and the activity of related enzymes SOD, POD and CAT are reduced; and the expression of down-regulation StERF1A The opposite effect is presented, which significantly enhances the resistance of potato to bacterial wilt. The present application provides an important gene target and theoretical basis for breeding new potato varieties with high resistance to bacterial wilt.

The Bacillus velezensis M173 was deposited with the Guangdong Microbial Culture Collection Center with a deposit number of GDMCC No. 61434. After being prepared into a seed-coating agent, Bacillus velezensis M173 can promote the growth and germination of plants and significantly improve the control effect on pathogens. Moreover, Bacillus velezensis M173 can relieve the condition of dead seedlings of crops at seedling stage after being applied to the crops. In particular, both pot experiments and field experiments prove that the Bacillus velezensis M173 of the present invention has a significant control effect on fungal and bacterial diseases, in particular, bacterial wilt and stem rot. Therefore, the Bacillus velezensis M173 of the present invention has broad application prospects.

This invention belongs to the field of fermentation engineering and plant disease control technology, specifically relating to the application of a Bacillus endophyticus compound inoculant fermentation broth in the control of plant diseases. The Bacillus endophyticus compound inoculant disclosed in this invention exhibits antibacterial effects against anthracnose in peppers, blight in peppers, gray mold in tomatoes, wilt in watermelons, wilt in cucumbers, verticillium wilt in cotton, black spot in peppers, root rot in carrots, soft rot in taro, and bacterial wilt in peppers, with inhibition rates of (75.37±1.44), (71.00±1.41)%, (83.14±0.96)%, (74.70±1.09)%, (69.21±1.04)%, (78.31±1.39)%, (73.06±0.73)%, (75.49±0.89)%, (46.79±1.57)%, and (73.82±0.51)%, respectively.

This invention belongs to the field of biotechnology. To improve the effectiveness of biological control of plant diseases, this invention discloses *Bacillus velezensis* ZF516 and its application in controlling various soil-borne diseases. The *Bacillus velezensis* strain number is ZF516, and its registration number at the China General Microbiological Culture Collection Center is CGMCC No. 30136. This *Bacillus velezensis* strain can prevent and / or treat tomato bacterial canker, tomato bacterial wilt, or cowpea root rot, or can be used to prepare products for the prevention and / or treatment of these diseases. This strain also exhibits good inhibitory effects against *Fusarium oxysporum*, *Diplostomum spp.*, *Rhizoctonia solani*, *Fusarium solanum*, *Corynebacterium multiflorum*, *Botrytis cinerea*, *Agrobacterium tumefaciens*, *Pectinobacter brasiliensis*, *Ralstonia solanacearum*, *Streptomyces spp.*, etc.

The application discloses application of a tobacco NtMATE34 gene and protein in regulation of a bacterial wilt resistance. The tobacco NtMATE34 gene is induced to express by inoculation of a bacterial wilt pathogen Ralstonia solanacearum, and participates in a tobacco bacterial wilt stress response. After artificial inoculation of the bacterial wilt, compared with K326, the tobacco plant with overexpression of the NtMATE34 gene shows strong bacterial wilt resistance. The tobacco NtMATE34 gene plays an important role in regulation of the tobacco bacterial wilt resistance, and can be applied to gene function research and biological breeding for improvement of the tobacco bacterial wilt resistance.

This invention relates to the field of biotechnology, specifically to *Bacillus belyssiensis* NH4 / 2 and its applications. This invention provides *Bacillus belyssiensis* NH4 / 2 and its applications. The *Bacillus belyssiensis* NH4 / 2 provided by this invention exhibits strong broad-spectrum inhibitory effects against pathogens such as bacterial wilt, red spot pathogen, tobacco black shank pathogen, anthracnose pathogen, wilt pathogen, and gray mold pathogen. Furthermore, it is isolated from soil and is harmless to humans, livestock, and the environment, meeting the needs of green plant protection and sustainable agricultural development.

This invention discloses a myxobacterium resistant to plant pathogenic fungi and bacteria. The strain is named *Myxococcus sp.* R23, with accession number GDMCC No: 63212. The methanol extract of the fermentation supernatant of *Myxococcus sp.* R23 inhibits the growth of *Rhizoctonia solani* Kühn GDMCC 3.513, which causes rice sheath blight. Furthermore, this strain can prey on *Ralstonia solanacearum* GIM 1.70, which causes bacterial wilt in tomatoes, demonstrating its application value in the biological control of plant diseases.

The application provides a kind of lipopeptide compound PW-1 and its preparation method and application in preventing and controlling bacterial wilt, belong to biological pesticide technical field;The PW-1 is prepared by fermentation, solvent extraction, acidification and reverse phase chromatography from Paenibacillus polymyxa (P. Paenibacillus polymyxa ) strain DW-7A;The core of the compound is that it plays a role through "anti-toxicity" mechanism, at the concentration without inhibiting the growth of Pseudomonas solanacearum (P. Ralstonia solanacearum ), can specifically interfere with the biosynthesis of pathogenic bacteria flagellum, cause its motility, chemotaxis and early infection ability to decrease significantly;Pot experiment proves that the relative control efficiency of PW-1 to tobacco bacterial wilt reaches 62.2%, and has no effect on the growth of soil bacillus at the concentration of up to 1000 μg / mL, shows efficient, targeted and environment compatible characteristics;The application provides a new candidate compound and key technical support for the development of green pesticide targeting flagellum.

The present application relates to the field of microbiological technology, and particularly relates to a walnut decay disease biocontrol bacterium Achromobacter insuavis P52 and application thereof.The Achromobacter insuavis is Achromobacter insuavis P52, which is preserved in the China General Microbiological Culture Collection Center, and the preservation number is CGMCC No.28849.A strain of Achromobacter insuavis is isolated from a pot seedling of Populus trichocarpa, and the strain has a significant antagonistic effect on a walnut decay disease pathogenic fungus Cytospora chrysosperma, with an inhibition rate of 62.4%, and an in-vitro prevention and treatment effect on walnut branch decay disease of 76%.The Achromobacter insuavis in the present application has a significant prevention and treatment effect on walnut decay disease, and has a good application prospect.

This invention discloses the application of the natural product santalol as an agricultural fungicide in plant disease control, belonging to the field of plant disease prevention and control. It shows good inhibitory effects against bacterial diseases such as rice bacterial leaf blight, rice bacterial leaf streak, citrus canker, cabbage black rot, tomato bacterial spot, kiwifruit canker, tobacco bacterial wilt, cucumber bacterial angular leaf spot, and potato bacterial wilt. It also shows good inhibitory effects against viral diseases such as tobacco mosaic virus; and against fungal diseases such as rice rhizome blight, wheat scab, pepper wilt, *Botrytis cinerea*, or *Colletotrichum sorghum*. It can be used in the preparation of plant fungicides. Santalol is a natural plant extract and is environmentally friendly. This invention provides a new use for the natural product santalol, enabling its application in the agricultural field for plant disease control and providing a new avenue for the research and development of new pesticides.

The application discloses cupriavidus CUP3, a combination of cupriavidus CUP3 and raoultella ornithinolytica RAO1 and application of the combination in prevention and control of crop bacterial wilt. Cupriavidus necator Cupriavidus pinatubonensis Cupri The application discloses cupriavidus CUP3, a combination of cupriavidus CUP3 and raoultella ornithinolytica RAO1 and application of the combination in prevention and control of crop bacterial wilt. The application discloses cupriavidus CUP3, a combination of cupriavidus CUP3 and raoultella ornithinolytica RAO1 and application of the combination in prevention and control of crop bacterial wilt. The application discloses cupriavidus CUP3, a combination of cupriavidus CUP3 and raoultella ornithinolytica RAO1 and application of the combination in prevention and control of crop bacterial wilt.

This invention provides a method for promoting tobacco growth and preventing bacterial wilt. Rhodococcus qingshengii This invention relates to the field of microbial technology, specifically to Rhodococcus faecalis strain FJ5, with the preservation number CGMCC No. 32631. The FJ5 strain exhibits a wide adaptability range, growing well within a temperature range of 16–34°C and a pH range of 5.0–8.0. The FJ5 strain promotes early growth and enhances stress resistance in tobacco by mitigating aluminum stress, increasing the activity of tobacco defense enzymes, improving tobacco growth hormone secretion and carbon and nitrogen metabolism, while simultaneously improving acidic soil fertility and controlling tobacco bacterial wilt. The Rhodococcus faecalis FJ5 provided by this invention has significant development value for high-quality tobacco development under acidified soil conditions in rice-tobacco rotation, and is also important for improving plant stress resistance and the biological control of root and stem diseases.

The application provides a primer, a probe and application of an RPA-gold colloidal lateral flow immunochromatography method for detecting tobacco bacterial wilt bacteria. The primer comprises a specific primer pair designed for a tobacco bacterial wilt bacteria genome ABNQ24_03690-lpxC gene interval sequence; the probe comprises a gold nanoparticle combined probe, a quality control line capture probe and a tobacco bacterial wilt bacteria capture probe. Meanwhile, application of the primer and the probe in preparation of a tobacco bacterial wilt bacteria detection product and a detection method are provided. The specific primer and the probe designed for the tobacco bacterial wilt bacteria genome ABNQ24_03690-lpxC gene interval sequence are fused with the rapid isothermal amplification technology of RPA and the visual detection advantage of the gold colloidal lateral flow immunochromatography, rapid, high-sensitivity and high-specificity detection of the tobacco bacterial wilt bacteria is realized, complex instruments and professional operations are not needed, and the method is suitable for early, convenient and rapid diagnosis in multiple scenes.

The application discloses a pseudomonas and an application thereof, and belongs to the field of microbial application. The strain is preserved in the China General Microbiological Culture Collection Center (CGMCC) on November 28, 2025, and the preservation number is CGMCC NO. 36820. The strain belongs to the genus Pseudomonas sp., and the strain name is Pseudomonas T9. The strain has a significant inhibiting effect on plants infected with bacterial bacterial wilt disease bacteria, and has important application potential in the prevention and control of bacterial wilt disease.

This invention discloses a composition of *Brachystomum clavatum* CUP3, *Brachystomum clavatum* CUP3, and *Raoultella ornithine-lysinogen* RAO1, and its application in controlling bacterial wilt in crops. *Brachystomum clavatum* CUP3 is classified as... Cupriavidus yeoncheonensis The invention discloses the application of *Cup3*, a copper-loving bacterium, in the control of soil-borne bacterial wilt in crops. The invention also discloses a bacterial composition containing *Cup3* and *Rauvolfia ornithine-releasing* RAO1, or a fermentation broth containing *Cup3* and *Rauvolfia ornithine-releasing* RAO1; wherein the preservation number of *Rauvolfia ornithine-releasing* RAO1 is GDMCC NO:67513. Both *Cup3* and the bacterial composition can reduce the occurrence of bacterial wilt in tomatoes, especially the bacterial composition, which can significantly reduce the occurrence of bacterial wilt in tomatoes.

Abstract:　Problem: To provide a soil-transmissible plant disease control agent or similar that can safely be used as a biological control agent.　Solution: A soil-transmissible plant disease control agent or similar, such as a vegetable bacterial wilt control agent, can be provided by using, as an active ingredient, live bacterial cells of a novel strain of a bacterium of the Bacillus or Fictibacillus genus with a heretofore unknown soil-transmissible plant disease controlling action, or a cultured product including said live bacterial cells. This control agent is characterized by being able, through a foliage application treatment, to control bacterial wilt and / or bacterial soft rot caused by the Ralstonia solanacearum species complex, which are soil-borne bacteria.　Selected drawing: none

The present application relates to a kind of can prevent and treat bacterial wilt of lemon acid-loving Leuconostoc Q2-1, on February 9, 2026, be preserved in China Microorganism Strain Preservation Management Committee, and the application also relates to the application of the lemon acid-loving Leuconostoc Q2-1 in the prevention and treatment of bacterial wilt.The antagonistic strain Q2-1 of the present application is isolated from the rhizosphere of plant, and it is a kind of harmless strain to human and animal and plant, and the strain has significant antagonism to ralstonia solanacearum.In addition, volatile metabolites of antagonistic strain Q2-1 are analyzed by its plate bacteriostatic analysis, and it is found that certain metabolites in the gas produced by antagonistic strain Q2-1 have inhibitory effect on bacterial wilt pathogen CQPS-1.Therefore, the antagonistic strain Q2-1 of the present application has prevention and treatment effect on bacterial wilt of solanaceae crops, provides a new effective way for the prevention and treatment of bacterial wilt of solanaceae crops, and has good application prospect.

This invention discloses a Bacillus microbial agent for controlling tobacco diseases, its preparation method, and its application. The microbial agent is prepared by compounding Bacillus amyloliquefaciens and Bacillus bellis in a volume ratio of 0.8-1.2:1, resulting in a total effective viable count ≥2×10¹⁰ CFU / mL. This invention utilizes independently isolated specific Bacillus amyloliquefaciens and Bacillus bellis; the compounding of these two agents creates a synergistic effect. Bacillus amyloliquefaciens secretes antibacterial substances that directly inhibit the growth of pathogens, while Bacillus bellis induces resistance in tobacco plants and enhances rhizosphere colonization, synergistically improving the control effect against tobacco bacterial wilt and black shank. Field trials show that this microbial agent achieves a control efficacy of over 75% against tobacco bacterial wilt and over 85% against black shank, significantly higher than single-strain agents. The strains used in this invention are isolated from the rhizosphere soil of tobacco plants in different tobacco-growing areas of Baoshan. The addition of a special stabilizer after compounding enhances the strains' resistance, extends shelf life at room temperature, and maintains stable efficacy.

This invention discloses a strain of Acinetobacter piterum and its application in the control of bacterial wilt in tomatoes. The Acinetobacter piterum provided by this invention (… Acinetobacter pittii Acinetobacter pituitaria BB307, with accession number GDMCC NO. 67769, exhibits broad-spectrum antibacterial activity against eight sequence variants of Ralstonia solanaceae, making it suitable for controlling bacterial wilt in tomatoes. Furthermore, it possesses nitrogen-fixing, phosphorus-solubilizing, siderophore-producing, and indoleacetic acid-promoting abilities, which can promote plant growth in tomatoes. The Acinetobacter pituitaria BB307 provided by this invention demonstrates synergistic potential for biocontrol and growth promotion, offering a highly efficient microbial resource for green production of solanaceous crops and providing a more economical, efficient, and environmentally friendly solution for the prevention and control of bacterial wilt.

This invention discloses the application of isocitric acid in the control of bacterial wilt in plants, wherein the isocitric acid is derived from tomato root exudates. The inhibitory effect of isocitric acid on *Ralstonia solanacearum* was evaluated through shake-flask culture experiments and plate inhibition zone experiments, and the control effect of isocitric acid on bacterial wilt was determined using greenhouse pot experiments. The results showed that within the concentration range of 10–200 μM, isocitric acid significantly inhibited the growth of *Ralstonia solanacearum*, forming a distinct inhibition zone. Under greenhouse pot conditions, applying 0.2–0.5 mg / kg of isocitric acid to the soil effectively reduced the incidence of bacterial wilt in tomatoes, with a disease control rate of 21.43%–52.38%. Furthermore, isocitric acid can indirectly inhibit the occurrence of bacterial wilt by influencing the soil bacterial community. The isocitric acid used in this invention is derived from natural plant root exudates, possessing advantages such as environmental friendliness and high safety, and can be used as a novel inhibitor of tomato bacterial wilt, showing promising application prospects in the field of green control of plant diseases.

This invention discloses a Streptomyces strain AB_3, classified as amylase-producing Streptomyces (…). Streptomyces diastatochromogenes The strain is preserved under the CCTCC No. M20252932. This invention also discloses a solid-state fermentation agent of *Streptomyces AB_3*, comprising the aforementioned *Streptomyces AB_3* strain. This invention further discloses the application of *Streptomyces AB_3* strain or the solid-state fermentation agent of *Streptomyces AB_3* in controlling soil-borne bacterial wilt and in promoting crop growth. *Streptomyces AB_3* possesses multiple functions, including disease resistance and growth promotion. This invention utilizes a novel solid-state fermentation method, significantly increasing the amount of *Streptomyces* spores and solving the problem of *Streptomyces* failing to produce spores during liquid culture.

The application discloses a kind of Streptomyces for preventing and treating tomato bacterial disease, fermentation liquor and preparation method and application, and the Streptomyces for preventing and treating tomato bacterial disease is Streptomyces venezuelae FK-11, is preserved in China Microbial Culture Collection Center, the preservation number is CGMCC NO.25330, the preservation date is July 18, 2022, and the preservation address is No.3, Xili, Beichen, Beijing City, Chaoyang District.Compared with the prior art, the application provides a kind of soil antagonistic bacteria, which is a kind of Streptomyces that can produce chloramphenicol, isolated and selected from the surrounding of tomato root base, the fermentation liquor can effectively inhibit tomato bacterial disease, especially tomato bacterial canker disease and tomato bacterial wilt, it is derived from soil, and has strong environmental adaptability, and can effectively improve yield while inhibiting disease occurrence, the mode of action is fermentation liquor seed soaking, easy to operate, time-saving and labor-saving, environment-friendly, hope to replace chemical medicine, reduce chemical input, reduce environmental pollution, and guarantee the green and efficient production of tomato.

This invention provides a wettable powder of DSE (dichloropyrophylloides) spores, its preparation method, and its application, belonging to the field of microbial preparation technology. It comprises chlamydospores from at least one dark-colored septate endophytic fungus, a carrier, and excipients, wherein the chlamydospore content is greater than 2.35 × 10⁻⁶. 8 CFU / g. This invention improves the properties of wettable powder by rationally optimizing the dosage of DSE chlamydospores and excipients, achieving a chlamydospore content of 2.35 × 10⁻⁶ CFU / g in the finished product. 8 With a concentration of CFU / g, a wetting time of 24.25s, and a suspension rate of 73.8%, all indicators meet national standards. It can be stored at room temperature for over 12 months, extending its shelf life by more than 6 months compared to conventional conidiophore agents. This powder significantly increases the expression of five defense enzymes—PAL, PPO, POD, CAT, and SOD—in tomato and rice plants, thereby activating the plant's defense system. It effectively controls bacterial wilt in tomatoes and black-streaked dwarf disease in southern rice, improving the growth of tomatoes infected with bacterial wilt and rice infected with black-streaked dwarf disease. It is suitable for promotion and use as a novel biological pesticide product.

This application relates to the field of green control technology for rice diseases and pests, specifically disclosing a method for synergistic reduction of pesticide, microbial, and fertilizer control in rice based on coconut shell fermentation broth. The composition includes chlorantraniliprole suspension, tricyclazole wettable powder, matrine aqueous solution, compound microbial agent, brassinolide emulsifiable concentrate, and coconut shell fermentation broth, wherein the compound microbial agent has an effective viable count of not less than 1×10⁻⁶. 8 The total free amino acid content of the coconut shell fermentation broth is not less than 5% (CFU / mL). The coconut shell fermentation broth is prepared using a segmented anaerobic directional fermentation process, involving raw material pretreatment, two-stage stepwise fermentation, and post-treatment. This composition can be used for the control of target pests and diseases in rice fields under high temperature and humidity conditions. Through the complementary functions and synergistic effects of multiple components, it achieves simultaneous and efficient control of multiple target pests and diseases in rice while significantly reducing the amount of chemical pesticides used. It also considers rice growth regulation and field ecological safety, meeting the production needs of green pest control in rice.

The application discloses application of baicalein in prevention and control of soil-borne bacterial wilt. The application discloses application of baicalein in preparation of a preparation for preventing and controlling soil-borne bacterial wilt. The application discloses a method for preventing and controlling soil-borne bacterial wilt, which comprises the following steps: after transplanting seedlings of plants for 7-10 days, baicalein solution is applied by means of root irrigation. In the application, baicalein is applied by means of root irrigation, which can promote secretion of bacteriostatic substances of beneficial bacterial groups in the rhizosphere of solanaceous crops such as tomatoes, inhibit bacterial wilt, reduce the incidence of tomato bacterial wilt, and prevent and control soil-borne bacterial wilt.

The application discloses application of a SmCYP82D47 gene in improving resistance of eggplants to bacterial wilt, and belongs to the technical field of molecular biology. The nucleotide sequence is shown as SEQ ID NO. 2. On the basis of GWAS positioning of an eggplant bacterial wilt resistance linkage site, the application screens an eggplant bacterial wilt resistance candidate gene SmCYP82D47 through bioinformatics analysis such as gene sequence analysis and haplotype analysis; analyzes tissue expression specificity and hormone response expression characteristics of the candidate gene; and observes the disease resistance of a silencing plant and a wild type plant, and the disease resistance of a wild type plant and an overexpression plant by silencing the candidate gene in a resistant material S027 and overexpressing the candidate gene in a susceptible material S054, so as to not only verify the accuracy of the positioning result and determine the role of the candidate gene in regulating the bacterial wilt resistance of the eggplant, but also provide an important theoretical basis and technical means for eggplant disease resistance molecular breeding.