357 results about "Bacterial wilt" patented technology

The invention provides a Pseudomonas protegens P150 strain and an application of the Pseudomonas protegens P150 strain, and belongs to the technical field of biocontrol bacteria. The defensive pseudomonas P150 is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC No.34520, the preservation time is May 13, 2025, and the preservation address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The defensive pseudomonas P150 disclosed by the invention has the effects of inhibiting various pathogenic bacteria, producing enzymes, promoting growth and preventing and treating tomato bacterial wilt and soybean pustule. The microbial inoculum prepared by adopting the biocontrol bacterium can reduce the dosage of chemical pesticides and the prevention and control cost of plant diseases, is green and pollution-free, can be used for preventing and controlling tomato bacterial wilt and soybean pustule, and has a relatively good popularization value.

The invention provides Yunnan golden camellia root endophytic fungi and application thereof, and relates to the technical field of crop disease control. The Yunnan camellia nitidissima root endophytic fungus is Lecanicillium vase JHC098 and is preserved in the China Center for Type Culture Collection, the preservation number is CCTCC NO: M 2025749, and a liquid fermentation liquor crude extract of the Lecanicillium vase can be applied to prevention and treatment of crop diseases. The invention overcomes the defects in the prior art, and the crude extract of the liquid fermentation liquor of the endophytic fungus Lecanicillium viculare JHC098 at the root of Yunnan golden camellia can effectively inhibit the growth of pathogenic bacteria such as citrus canker and tobacco bacterial wilt, thereby achieving the purpose of certain biological control.

The invention discloses bacillus velezensis XF-8 and application of the bacillus velezensis XF-8 in prevention and treatment of tomato bacterial wilt caused by Ralstonia solanacearum, and belongs to the technical field of microorganisms. The bacillus velezensis XF-8 capable of antagonizing Ralstonia solanacearum is obtained through separation and screening, and it is found that the bacillus velezensis XF-8 also has an excellent inhibition effect on various pathogenic fungi and shows broad-spectrum antibacterial activity of the bacillus velezensis XF-8. The bacillus velezensis XF-8 has a good inhibition effect on tomato bacterial wilt, and the control effect of the bacillus velezensis XF-8 in potted tomatoes reaches 74.25%. Results of the invention show that the bacillus velezensis XF-8 has a great application prospect in prevention and treatment of tomato bacterial wilt, and a good biocontrol resource is provided for research and development of a biocontrol agent for tomato bacterial wilt.

The invention discloses Delftia tsuhuensis and an application of the Delftia tsuhuensis in prevention and treatment of plant diseases. The Delftia tsuhuensis CDJ2 is preserved in the Guangdong Microbial Culture Collection Center on October 18, 2024, and the preservation number is GDMCC (China General Microbiological Culture Collection Center) NO: 65290. The strain has a good prevention and treatment effect on citrus canker, and the prevention and treatment effect can reach 84%. Meanwhile, the strain can also effectively prevent and treat soft rot of taro, bacterial basal rot of rice, bacterial wilt of crops, kiwifruit canker, angular leaf spot of mango, black rot of brassicaceous vegetables, tomato scab, gummosis of sugarcane, bacterial wilt of walnuts and bacterial leaf spot of plums. Wide application prospects are realized in the aspects of preventing and treating diseases of crops and commercial crops and promoting healthy growth of plants.

The invention provides a microbial agent type bio-organic fertilizer capable of improving continuous cropping tobacco planting soil hardening and a preparation method of the microbial agent type bio-organic fertilizer, and belongs to the technical field of organic fertilizers. The microbial agent type bio-organic fertilizer capable of improving continuous cropping tobacco planting soil hardening comprises the following raw materials: an organic material, a microbial agent, an inorganic nutritional ingredient, vinasse and an additive, wherein the organic material is composed of livestock and poultry manure, crop straw, tobacco waste and a fermentation inoculant; the additive is prepared from humic acid, seaweed extract and amino acid. The organic fertilizer contains rich organic materials and humic acid, the content of organic matters in soil can be increased, the granular structure of the soil can be improved, the growth of soil-borne pathogenic bacteria can be inhibited by the added multiple beneficial microorganisms, the micro-ecological environment of the soil can be improved by the photosynthetic bacteria, and the incidence rate of diseases such as tobacco black shank and bacterial wilt can be reduced.

The invention provides a ginger planting method for preventing and treating ginger bacterial wilt, and belongs to the technical field of ginger planting.According to the ginger planting method, through crop rotation, a ginger bacterial wilt pathogenic bacterium host chain is effectively cut off, ralstonia solanacearum is inhibited, harmful pathogenic bacteria in soil are removed through sun exposure, a biochar-probiotic mixture is applied, and meanwhile the harmful pathogenic bacteria in the soil are inhibited; biochar is utilized to increase porosity and water-holding capacity, relieve the problem of soil hardening, increase the pH value of soil, increase the content of alkaline cations, effectively inhibit growth of pathogenic bacteria of ginger blast, and also adsorb and retain nutrients such as ammonium and potassium through static electricity, and the pore structure of the biochar provides a shelter for probiotic groups, so that the probiotic groups are planted in the soil, and the soil quality is improved. The living space of the ginger blast pathogenic bacteria is inhibited; seed gingers are soaked in bacterium liquid containing bacteriophages, ginger blast pathogenic bacteria are effectively inhibited, drip irrigation is adopted for later-period management to maintain soil humidity, reproduction and spreading of the ginger blast pathogenic bacteria are effectively prevented and controlled, charcoal base fertilizer is additionally applied, and finally ginger blast is effectively prevented and controlled.

The invention discloses a crop ralstonia solanacearum disease prediction method based on an integrated learning model, and the method comprises the following steps: S1, collecting a published 16s rRNA gene sequence related to solanaceae crop bacterial wilt, and carrying out the preprocessing of original sequencing data based on an EasyAmplicon standardized process; s2, performing data dimension reduction by using a principal component analysis algorithm, and retaining 95% of variance; s3, performing hyper-parameter search based on 5-fold cross validation and grid search on the Light GBM model, the CatBoost model and the XGBoost model respectively, and selecting three groups of optimal hyper-parameters of each model; s4, constructing a model according to three groups of optimal hyper-parameters of each selected model, performing prediction, analyzing model result difference based on a Pearson correlation coefficient, and retaining Pearson correlation coefficient mean < lt > with other eight model prediction values; a model of 0.8; and S5, inputting the screened model prediction result into a second-layer element learner RF for integrated learning to obtain a final prediction result.

The invention discloses bacillus velezensis BR-1 and application of the bacillus velezensis BR-1 in prevention and treatment of economic crop diseases. The preservation number of the bacillus velezensis BR-1 is CCTCC NO: M 20251379. The bacillus velezensis BR-1 disclosed by the invention is high in adaptability and wide in growth condition range, and is more beneficial to application in agricultural production. Meanwhile, the bacillus velezensis BR-1 has a remarkable inhibition effect on the growth of various phytopathogens such as verticillium dahliae, fusarium oxysporum, sclerotinia sclerotiorum and ralstonia solanacearum, can remarkably reduce the morbidity of cotton verticillium wilt, fusarium wilt and tomato bacterial wilt under a greenhouse condition, and has a wide agricultural application prospect.

The invention discloses an application of an NtWRKY59 gene in regulation and control of tobacco bacterial wilt resistance. A nucleotide sequence of the NtWRKY59 gene is shown as SEQ ID NO.1, and the NtWRKY59 gene is over-expressed in tobacco. The invention further discloses an application of the NtWRKY59 gene in regulation and control of tobacco bacterial wilt resistance. The NtWRKY59 gene provided by the invention not only enriches the genetic basis of tobacco bacterial wilt resistance as a brand new anti-disease gene resource, but also provides a new thought for developing a disease-resistant breeding strategy different from an upstream signal element as an action mechanism of a downstream transcription factor, and has good scientific research value and application prospect.

Bacteriophages that comprise a tail fiber protein sequence comprising a C terminus host recognition site described by SEQ ID NO: 1 and which infect Ralstonia solanacearum which causes bacterial wilt disease are disclosed. Compositions comprising these bacteriophages may be employed as wilt control agents. Methods for preventing or treating diseases caused by Ralstonia solanacearum such as bacterial wilt disease are also disclosed. A bacterial wilt disease control method in a plant may include administering such bacteriophages to a plant or to a plant growth medium and can be effective against a wide variety of Ralstonia solanacearum strains. Nucleic acids encoding the C terminus host recognition site and amino acid sequences comprising the host recognition site are also disclosed.

The invention belongs to the technical field of biocontrol bacteria, and discloses bacillus velezensis and application thereof in preventing and treating bacterial wilt of solanaceae crops. The bacillus velezensis is named as bacillus velezensis PR-7, and is preserved in the China General Microbiological Culture Collection Center (CGMCC), the address of the preservation unit is No.3, No.1 yard, Beichen West Road, Chaoyang District, Beijing, the preservation time is November 13, 2023, and the preservation number is CGMCC No.28979. The bacillus velezensis has the advantages that the bacillus velezensis is named as bacillus velezensis PR-7; the bacillus velezensis can effectively inhibit the growth of a bacterial wilt pathogen, i.e., the evolutionary I type (main evolutionary type of bacterial wilt pathogen in South China) of bacterial wilt pathogen, improves the bacterial wilt resistance of solanaceae crops, and is of great significance to the prevention and treatment of bacterial wilt of solanaceae crops in South China.

The invention discloses application of cobalamin and beneficial bacteria in prevention and control of tomato soil-borne bacterial wilt. The beneficial bacterium is Chryseobacterium CHR6, and the preservation number of the strain is CGMCC (China General Microbiological Culture Collection Center) The invention discloses a synbiotics for preventing and controlling tomato soil-borne bacterial wilt. The synbiotics consist of beneficial bacteria and cobalamin, and the beneficial bacterium is Chryseobacterium CHR6. The invention discloses a method for preventing and controlling tomato soil-borne bacterial wilt by matching cobalamin and beneficial bacteria, which comprises the following steps: 7-10 days after transplanting tomato seedlings, adding synbiotics into soil by a root irrigation method, adding 10-20 mL of cobalamin solution with the concentration of 5-10 nM into each tomato seedling, and inoculating 10-20 mL of beneficial bacteria suspension with the concentration of 1 * 10 < 7 >-1 * 10 < 9 > CFU / mL. By adding cobalamin, inhibition of growth of ralstonia solanacearum through siderophore mediation of Chryseobacterium CHR6 can be remarkably enhanced, and occurrence of tomato soil-borne bacterial wilt is reduced.

The invention relates to a ralstonia solanacearum bacteriophage LC10 capable of preventing and controlling tobacco bacterial wilt, the classification name of the ralstonia solanacearum bacteriophage LC10 is Rastonia phage, the preservation number of the ralstonia solanacearum bacteriophage LC10 is GDMCC No: 66716-B1, and the preservation date of the ralstonia solanacearum bacteriophage LC10 is July 21, 2025. The bacteriophage is a brachytail bacteriophage and can specifically split ralstonia solanacearum, inhibit the growth of the ralstonia solanacearum and enhance the disease inhibition capability of soil. Therefore, the bacteriophage LC10 can be used for preparing related preparations for preventing and controlling tobacco bacterial wilt.

The invention belongs to the technical field of microorganisms. The invention relates to a coxakella cocori Kos-10 strain, in particular to a coxakella cocori Kos-10 strain and application thereof. The invention discloses a Cosakya cokei Kos-10 strain which is preserved in the Guangdong Microbial Culture Collection Center on June 5, 2025, and the preservation number of the Cosakya cokei Kos-10 strain is GDMCC (China General Microbiological Culture Collection Center) NO: 66468. The Cosakya cokei Kos-10 strain has been preserved in the Guangdong Microbial Culture Collection Center on June 5, 2025. The strain has the capabilities of fixing nitrogen, dissolving phosphorus, dissolving potassium and producing siderophores, can be used as a growth-promoting bacterium for promoting plant growth, provides nutrient elements of nitrogen, potassium and iron for plants, and also can prevent and treat bacterial wilt of the plants. The Kos-10 strain disclosed by the invention is comprehensive in growth promoting effect, and has a good application prospect and application value in the aspect of promoting plant growth under the stress of bacterial wilt.

The invention discloses an application of an NtWRKY41 gene in regulation and control of tobacco bacterial wilt resistance. A nucleotide sequence of the NtWRKY41 gene is shown as SEQ ID NO.1, and the NtWRKY41 gene is over-expressed in tobacco. The invention further discloses an application of the NtWRKY41 gene in regulation and control of tobacco bacterial wilt resistance. The NtWRKY41 gene provided by the invention not only enriches the genetic basis of tobacco bacterial wilt resistance as a brand new anti-disease gene resource, but also provides a new thought for developing a disease-resistant breeding strategy different from an upstream signal element as an action mechanism of a downstream transcription factor, and has good scientific research value and application prospect.

The invention provides a method for improving the disease resistance of plants, and particularly provides a method for improving the disease resistance of the plants by reducing or inhibiting the expression quantity and / or activity of SWEET11a genes or encoded proteins thereof. According to the method, the disease resistance of the plants can be improved, particularly the resistance to gray speck disease, northern leaf blight, bacterial wilt and stem rot is improved, and the method has important application value in biological breeding.

The invention discloses enterobacter hormaechei and application thereof, and belongs to the technical field of microorganisms. The strain is named as Enterobacter hormaechei (Enterobacter hormaechei) zhegui 61401, is preserved in the China Center for Type Culture Collection (CCTCC), and has the preservation number of CCTCC NO: M 20241827. The enterobacter hormaechei provided by the invention has the effect of relieving the influence of acid stress on plants, can inhibit the growth of ralstonia solanacearum, can be applied to biological prevention and control of plant bacterial wilt in an acidified soil area, and expands the application range of the enterobacter hormaechei. The invention provides a feasible solution for sustainable production of tomatoes in the acidified soil area.

The invention discloses staphylococcus Z8 for antagonizing pathogenic bacteria of mulberry bacterial wilt and application of the staphylococcus Z8. The Z8 is identified as staphylococcus sp. And is preserved in Guangdong Microbial Culture Collection Center, the preservation number is GDMCC No.66232, and the preservation time is April 28, 2025. The invention further discloses a preparation method of the staphylococcus Z8 for antagonizing the pathogenic bacteria of mulberry bacterial wilt and application of the staphylococcus Z8 for antagonizing the pathogenic bacteria of mulberry bacterial wilt. The strain Z8 disclosed by the invention can destroy the formation of a biological membrane of the mulberry ralstonia solanacearum, so that the pathogenicity of the ralstonia solanacearum is reduced, and the strain Z8 can be used for preventing and treating the mulberry bacterial wilt. In addition, the strain Z8 does not affect the growth and development of the mulberry, the safety of the strain as a biocontrol strain is embodied, and the root activity and the total chlorophyll content can be improved. An efficient and environment-friendly microbial resource is provided for biological prevention and control of mulberry bacterial wilt, traditional chemical pesticides can be replaced, and environmental pollution is reduced.

The invention relates to the technical field of data processing, and discloses a peanut anti-bacterial wilt breeding strategy analysis method and system based on a growth cycle. The method comprises the following steps: acquiring and analyzing peanut growth period characteristic data to obtain a growth period characteristic library; performing phenotype comparison on healthy plants and diseased plants, and establishing an anti-bacterial wilt early warning model; performing real-time monitoring according to the early warning model to obtain a risk early warning result; performing growth period tracking based on an early warning result, and establishing a resistance scoring system; strain stability parameters are obtained through environmental response analysis; and finally determining excellent strains to form a bacterial wilt resistant breeding scheme. By establishing a data monitoring and analysis system in the whole growth period, dynamic evaluation and early warning of the bacterial wilt resistance of the plant are realized, and the accuracy and efficiency of bacterial wilt resistance variety breeding are improved.

The invention discloses an application of an NtWRKY26 gene in regulation and control of tobacco bacterial wilt resistance. A nucleotide sequence of the NtWRKY26 gene is shown as SEQ ID NO.1, and the NtWRKY26 gene is over-expressed in tobacco. The invention also discloses an application of the NtWRKY26 gene in regulation and control of tobacco bacterial wilt resistance. The NtWRKY26 gene provided by the invention not only enriches the genetic basis of tobacco bacterial wilt resistance as a brand new anti-disease gene resource, but also provides a new thought for developing a disease-resistant breeding strategy different from an upstream signal element as an action mechanism of a downstream transcription factor, and has good scientific research value and application prospect.

The invention discloses staphylococcus xylosus and application of the staphylococcus xylosus in antagonism of ralstonia solanacearum, and relates to the technical field of agricultural biological control, the staphylococcus xylosus CQZY-H-XJ-0002 provided by the invention is preserved in Guangdong Microbial Culture Collection Center on October 14, 2024, and the preservation number of the strain is GDMCC NO.65264. The staphylococcus xylosus CQZY-H-XJ-0002 provided by the invention is named as KQZY-H-XJ-0002. The staphylococcus xylosus CQZY-H-XJ-0002. The strain has a remarkable effect of inhibiting the growth of ralstonia solanacearum, and can effectively prevent and treat bacterial wilt and promote plant growth. The strain has the advantages of being simple in process, easy to operate, low in cost, green, safe, non-toxic and the like when being used for biological control.

The present invention discloses a fermentation method for Ralstonia solanacearum and a high-yield Ralstonia solanacearum phage, belonging to the field of microbial technology. The present invention obtains a new strain of Ralstonia solanacearum (Ralstonia solanacearum) BMB-Rs0001 by screening, and its preservation number is CCTCC NO: M20241876. This strain can significantly increase the explosiveness of Ralstonia solanacearum phage fermentation as a host bacteria. At the same time, the present invention improves the fermentation medium, fermentation temperature and feeding process, and ultimately greatly increases the fermentation yield of Ralstonia solanacearum phage, with the highest titer reaching 1.05×10 12 The Ralstonia solanacearum phage obtained by the method of the present invention significantly enhanced its storage stability at 4°C and 25°C over 180 days, and its control rate against bacterial wilt in plants increased from 72.82% to 90.51%.

The present invention discloses the application of coumarin-3-carboxylic acid in preventing and treating plant bacterial diseases. The coumarin-3-carboxylic acid provided by the present invention has a significant inhibitory effect on plant bacterial diseases. The plant bacterial diseases are oryzae bacterial leaf blight, oryzae bacterial leaf streak, citrus canker, cantaloupe bacterial fruit spot, tomato bacterial wilt, strawberry bacterial angular leaf spot, cabbage black rot, cabbage soft rot, potato soft rot, mango bacterial angular leaf spot, cassava bacterial wilt, cucumber bacterial angular leaf spot, kiwifruit canker and banana bacterial soft rot. The coumarin-3-carboxylic acid provided by the present invention has protective and therapeutic effects on melon bacterial fruit spot.

The invention discloses application of a SmCYP82D47 gene in improvement of eggplant bacterial wilt resistance, and belongs to the technical field of molecular biology. 1, and the nucleotide sequence of the gene is as shown in SEQ ID NO. 2. On the basis of positioning an eggplant bacterial wilt resistance linkage site by GWAS, the eggplant bacterial wilt resistance candidate gene SmCYP82D47 is screened through bioinformatics analysis such as gene sequence analysis and haplotype analysis; the tissue expression specificity, hormone response expression characteristics and the like of candidate genes are analyzed; by silencing candidate genes in a resistant material S027 and overexpressing the candidate genes in a susceptible material S054, the disease resistance conditions of a silent plant and a wild type plant as well as the wild type plant and an overexpressed plant are observed, so that the accuracy of a positioning result can be verified, and the effect of the candidate genes in regulating and controlling the bacterial wilt resistance of the eggplant is determined; important theoretical basis and technical means can be provided for eggplant disease-resistant molecular breeding.

The present invention discloses a method for batch inoculation of phages to control tobacco bacterial wilt, including: Step S1, culturing phages and Ralstonia solanacearum together to obtain phages with the ability to infect and lyse tobacco Ralstonia solanacearum; Step S2, encapsulating the phages into a nano-carrier to prepare nanoparticles, where the nano-carrier is a biodegradable nano-carrier; Step S3, cutting off water for hardening seedlings 5 - 7 days before transplanting flue-cured tobacco floating seedlings. After the hardening of seedlings is completed, put 2 - 3 cm of shallow water in the seedling-raising pool, add the phage-containing nanoparticles and an adhesive to the nutrient pool, and put the floating seedling trays back into the seedling-raising pool for inoculation. By combining phage therapy and nanotechnology, the present invention adds nano-encapsulated phages to tobacco floating seedling raising, which can not only inoculate a large number of tobacco seedlings more conveniently and quickly, but also extend the persistence of phages in the soil and improve their control efficacy.

The invention relates to the technical field of plant genetic engineering, in particular to a peanut bacterial wilt resistance related gene Ah6Q1KS5, an encoding protein thereof, a biological material and application of the gene Ah6Q1KS5 in disease-resistant breeding. Through whole genome identification and functional analysis, the gene Ah6Q1KS5 which is remarkably related to bacterial wilt resistance in a peanut SAMS gene family is screened, and the gene is induced by ralstonia solanacearum, salicylic acid (SA), abscisic acid (ABA) and methyl jasmonate (MeJA) to be remarkably expressed in a peanut bacterial wilt resistant variety H108. Peanut and tobacco plants over-expressing Ah6Q1KS5 can significantly reduce cell death, inhibit growth of ralstonia solanacearum, and enhance resistance to bacterial wilt. The molecular marker can be used for analyzing the molecular mechanism of peanut bacterial wilt resistance, provides new gene resources and technical means for cultivating bacterial wilt resistant peanut varieties, and has important application value in the field of peanut breeding.

The application discloses a staphylococcus and application of the staphylococcus in preparation of a control agent for tobacco black shank and tobacco bacterial wilt, relates to the technical field of microorganisms, and the staphylococcus 3C obtained by the application has obvious inhibiting effect on tobacco phytophthora and ralstonia solanacearum, and the fermentation liquor prepared from the staphylococcus 3C has obvious prevention and treatment effect on tobacco black shank and tobacco bacterial wilt.

The invention provides Rhodococcus qingshengii FJ5 capable of promoting tobacco growth and preventing and treating bacterial wilt and application of the Rhodococcus qingshengii FJ5, and belongs to the technical field of microorganisms. The invention provides a rhodococcus FJ5 strain, and the collection number of the rhodococcus FJ5 strain is CGMCC No.32631. The FJ5 strain is wide in application range, and can well grow under the condition that the temperature is 16-34 DEG C and the pH value is 5.0-8.0. The FJ5 strain promotes early growth of tobaccos and enhances stress resistance by relieving acid-aluminum stress, improving defensive enzyme activity of the tobaccos and improving secretion of growth hormones of the tobaccos and the carbon and nitrogen metabolism level of the tobaccos, and meanwhile, improves fertility of acid soil and prevents and treats bacterial wilt of the tobaccos. The Rhodococcus FJ5 provided by the invention has important development value in high-quality development of tobacco under the condition of rice-tobacco rotation acidified soil, and also has important significance in improvement of stress resistance of plants and biological control of root and stem diseases.

The invention belongs to the field of agricultural bactericides, and particularly relates to a bactericidal composition containing nano-zinc oxide and brucea javanica extract and application of the bactericidal composition, and the mass ratio of the nano-zinc oxide to the brucea javanica extract in active ingredients of the bactericidal composition is 40: 1-1: 40; the bactericidal composition disclosed by the invention has an excellent bactericidal effect, and shows excellent control effects on watermelon fusarium wilt, wheat stem rot, tobacco bacterial wilt, cotton damping-off, cotton damping-off, peanut root rot, peanut stem rot, damping-off, fusarium wilt, damping-off, root rot and the like of fruits, vegetables and beans; the bactericidal composition enhances the bactericidal broad-spectrum property and efficiency, is environment-friendly, does not generate phytotoxicity, is safe to crops, and has a good application prospect.

The present application relates to a strain of Streptomyces griseoaurantiacus and its application, belonging to the technical field of biological control of plant diseases. The Streptomyces griseoaurantiacus of the present application has been preserved in Guangdong Microbial Culture Collection Center on July 28, 2023, with the preservation number of GDMCC No: 63691 and the preservation address of No. 59 Building, 5th Floor, 100, Martyrs' Road, Guangzhou, Guangdong Province. The strain can inhibit the formation of Ralstonia solanacearum biofilm and the occurrence of Ralstonia solanacearum caused by bacterial wilt, has good prevention and control effect on bacterial wilt, and can be used as a biocontrol agent and biological preparation for the prevention and control of bacterial wilt.