866 results about "Bacterial wilt" patented technology

The invention relates to a trichoderma harzianum composite bacteria culture and application of the same in the aspect of plant protection, and belongs to the technical field of biology. The strain of the trichoderma harzianum composite bacteria culture is preserved in the China General Microbiological Culture Collection Center (CGMCC), and classified and named as Trichoderma harzianum MDCGTH 18, with a preservation number of CGMCC NO.2965. The Trichoderma harzianum MDCGTH 18 composite bacteria culture utilizes antagonistic microbes for biological prevention and treatment, simultaneously has the functions of inducing the disease resistance of plants and increasing the yield, has obvious effect of controlling aspen root rot, apple root rot, citrus yellow shoot, watermelon fusarium wilt, tobacco bacterial wilt, wheat scab, full rot, root rot, banded sclerotial blight, cucumber powdery mildew, downy mildew, vegetable gray mold, fusarium wilt and various other epidemic diseases, can be independently taken as a biological control microbial inoculum for plant diseases, and can also be mixed with other biological antiseptics, plant antibiotics, plant growth regulators and the prior antiseptics to prepare various biological antiseptics for controlling different plant diseases.

The invention provides bacillus amyloliquefaciens K-8 and bactericide thereof. The preservation number of the bacillus amyloliquefaciens K-8 strain is CGMCCNO.6486. The bactericide takes the bacillus amyloliquefaciens K-8 strain as an active ingredient. The bacillus amyloliquefaciens K-8 strain is cultured to obtain a liquid bactericide, and a solid bactericide can be obtained by adding a carrier or mixture of the carrier and auxiliaries into the liquid bactericide. The bacillus amyloliquefaciens K-8 strain and the bactericide thereof provided by the invention can be used for effectively preventing and treating diseases such as bacterial wilt, wilt, angular spot, banded sclerotial blight, rice false smut, soft rot, rot, canker and root rot. The bactericide is simple to prepare and easy to use, is applicable to industrialized production and has wide application prospect.

A microbial preparation for preventing and treating the bacterial wilt, damping-off, etc of plant and promoting growth of palnt contains paenibacillus polymyxa or the fermented liquid obtained by culturing the bacterium.

The invention provides a biocontrol bacterial strain for preventing and treating plant diseases and its bacterial agent, belonging to the field of biological control. The strains used are Gram-negative bacteria, identified as Lysobacter enzymogenes, and the strain code is OH11. Strain OH11 has no flagella but has slippage, can produce various extracellular hydrolytic enzymes including chitinase, β-1,3-glucanase and protease, and can effectively inhibit the growth of fungi and bacteria. The antibacterial zone diameters of strain OH11 against Sclerotinia sclerotiorum and Phytophthora capsici were both greater than 22.0 mm; the antagonism against potato ring rot was stronger, and the diameter of the inhibition zone reached 50 mm. The OH11 strain was inoculated into the seed tank, cultivated to the logarithmic growth phase, and the seed liquid was connected to the production tank for cultivation. The medium used in the production tank was the same as that of the seed tank. The liquid fermentation adopts aerobic submerged fermentation and fed-batch process, the dissolved oxygen is 15%-20%, the fermentation temperature is 30°C, the fermentation time is 72h, and the initial pH value is 7.5. After the fermentation is completed, the culture solution is taken out of the tank and directly packed into liquid dosage forms with plastic packaging barrels or packaging bottles, or subpackaged into solid dosage forms with peat adsorption packaging bags. The biocontrol strain OH11 can effectively control plant pathogenic fungi, bacteria, nematodes and other diseases, and the overall control effect is 50%-70%. In the greenhouse pot experiment, the control effects of OH11 on pepper blight and tomato bacterial wilt reached 83.6% and 86.4%, respectively. Strain OH11 has the characteristics of broad antibacterial spectrum, high activity, and environmental safety. In today's serious pesticide pollution, zymolysobacterium and its bacterial agent will be a good substitute.

The invention relates to antagonistic bacteria preventing and removing continuous cropping tomato bacterial wilt and a microbial organic fertilizer produced thereby, which belong to the technologies of agricultural intensified production. The antagonistic bacteria NJQL-18 with the significant antagonistic effect for pathogenic bacteria of the tomato bacterial wilt is obtained by separation, solidfermentation is further carried out on the antagonistic bacteria and organic materials for preparing the microbial organic fertilizer, the fertilizer contains more than 1 multiplied by 10<8> / g of NJQL-18, the content of total nitrogen is 4-5% (more than 90% is organic nitrogen), the total content of nutrients of nitrogen, phosphorus and potassium is 6-10%, and the content of organic matters is 30-35%. Tests show that the microbial organic fertilizer can lead the antagonistic bacteria to be rapidly propagated after being applied into the soil and form heterotic groups in the soil, and the biological prevention efficiency in the soil with the serious tomato bacterial wilt can be more than 80%. The long-term application of the microbial organic fertilizer in the soil without the formation ofcontinuous cropping obstacles can prevent the occurrence of the continuous cropping tomato bacterial wilt to a great extent.

The invention provides a liquid biological organic fertilizer with high content of organic substances and a preparation method. The fertilizer is characterized by comprising the following components by weight: 60% of a humic acid release inhibitor, 0.4% of a composite biological agent, 10% of fulvic acid potassium, 12% of soluble nitrogen-phosphorus-potassium raw materials, 9% of a chelating medium trace element agent, 8.47% of a soil conditioner, 0.93% of compound sodium nitrophenolate and 0.1% of naphthalene acetic acid; and the fertilizer is prepared by mixing, heating, chelating, concentrating, cooling, microorganism adding, filtering and residue removing. The invention has the following beneficial effects: plant nutrition is directly supplemented; the contents of absorbable organic substances for a root system in soil are rapidly supplemented; growth of the root system is rapidly promoted; absorption area of the root system is enlarged; the chelating medium trace element agent can not be fixed by the soil and exists in a free state in the soil, so the chelating medium trace element agent is easy to be captured and absorbed by the root system in the soil; physiological diseases and pests are prevented from occurrence; quality of an agricultural product is improved; root-rot disease and stem-rot disease are prevented from occurrence; occurrences of fusarium and verticillium wilts and bacterial wilt are reduced; meanwhile, the fertilizer is capable of resisting salinity and alkalinity, resisting continuous cropping diseases, and killing soil-borne diseases.

The invention relates to a seedling stage identification method for the resistance of tobacco to the bacterial wilt. The seedling stage identification method comprises the steps of heat preservation pool construction, pool water heating and circulation, wilt bacterium inoculation, tobacco seedling, disease state investigation and grading. The method replaces a conventional field identification result with a seedling stage identification result, identifies a great amount of material in a smaller area, carries out the identification a plurality of times annually, improves the reliability of the result, shortens the identification cycle, saves the site and test cost, and covers little floor space. The seedling stage identification method for the resistance of tobacco to the bacterial wilt is characterized by rapid disease development, good result repeatability, and the like. The seedling stage identification method for the resistance of tobacco to the bacterial wilt can effectively remove the interference on the resistance result caused by soil temperature difference, strain pathogenicity difference, strain quantity difference and other diseases, thereby benefiting the recurrence and comparison of results from different years and different labs. The difference between the attack rates of infection resistant varieties and the disease indexes is obvious; and the appraisal result of the resistance of the variety is basically consistent with the perennial resistance appraisal result obtained by others. The method has greater application potential in the resistance identification and resistance heredity rule study of disease resistant resources and breeding medium materials.

The invention provides a cold region paddy seedling culture matrix and relates to a paddy seedling culture matrix, and the cold region paddy seedling culture matrix is used for solving the problems of slow seedling emergence of the earlier stage, more irrigation times, serious damping off during the mid-to-late stage of seedling growing and obvious fertilizer removal during the later stage of the seedling when the existing paddy seedling culture matrix is applied in paddy cultivating areas of cold regions. The cold region paddy seedling culture matrix is prepared from edible fungus residues, coal ash, grass carbon, vermiculite and perlite, or ground straw, the coal ash, the grass carbon, the vermiculite and the perlite. The paddy which uses the cold region paddy seedling culture matrix does not need disease preventing and weeding during the seedling culture period, only needs to be irrigated, is convenient to operate, time-saving and labor-saving, has high quality seedlings and stronger stress resistance, no damping off, bacterial wilt and other diseases are caused during the seedling stage, and the seedlings are short and strong, have wide leaves and bright green color. The cold region paddy seedling culture matrix is applied in the paddy field.

The invention discloses bacillus amyloliquefaciens B011 for preventing and treating tobacco bacterial wilt and an application thereof, and relates to the technical field of biological prevention and treatment of plant diseases, wherein the bacillus amyloliquefaciens B011 has the preservation number of CCTCC NO:M2014576; separation comprises the following steps: firstly, sampling; secondly, grinding, thirdly, centrifugation; fourth, purification, and fifthly, screening. The bacillus amyloliquefaciens B011 has the characteristics of relatively good antagonistic effect on tobacco bacterial wilt, good safety on the environment and easy industrialized production, not only can be used for preventing and treating tobacco bacterial wilt, but also can be used for preventing and treating P.parasitica, A.alternata keissler, also can be used for preventing and treating C.capsici, P.capsici, C.gloeosprioides, P.oryzae, C.cuc-umerinum and other pathogenic fungi.

The invention relates to a bio-controlling bacterial strain AT31 for preventing and curing greenhouse vegetable root knot nematode disease and rice sheath blight disease, belonging to the technical field of plant protection. The bacterial strain AT31 is shaking cultured in PDA culture solution at 37 DEG C and 180rpm for 20-24 hours, then is subject to centrifugation at 6000rpm for 10min, a finished bactericide product thereof has a total viable bacteria concentration of 1*10<9>-1*10<10>cfu / ml, can be used by pouring at roots when transplanting vegetable seedlings after being diluted by 100 times, or be manufactured into microbial fertilizer to be used a week before the vegetable seedlings are transplanted, and has a prevention effect of 58-82% for root knot nematode disease of tomato, loofah, cucumber and other vegetables. The bactericide is diluted by 100 times to be used in spray treatment on rice stem base, has a prevention effect of 64.7% for rice sheath blight disease, and can increase the yield by 22.33%. And the bactericide can also cure vegetable bacterial wilt and epidemic diseases, and can improve soil hardening phenomenon.

The invention discloses a novel bacillus methylotrophicus, which has the preservation register number of CGMCC No.6793, wherein the strain is separated from tobacco rhizosphere soil and is obtained by separating and purifying and screening strains. The invention further provides a fermentation method of the bacillus methylotrophicus, a fermentation product prepared by the method, as well as a biological agent based on the fermentation product and a preparation method thereof; the invention further provides a use of the biological agent in controlling a variety of plant diseases. According to the invention, the biological agent is good in environmental compatibility so that the number of effective living spores is more than 5,000,000,000 / g, has high biological activity, and in particular has good control effect on tobacco bacterial wilt, wherein the control effect on tobacco bacterial wilt, soft rot of cabbage, rice bacterial leaf blight, rice bacterial stripe and cotton angular leaf spot is up to 80% or more.

The invention relates to a disease-preventing type special controlled-release fertilizer for Shandong peanuts as well as a preparation method and application thereof. The disease-preventing type controlled-release fertilizer is prepared from the following raw materials: humic acid coated urea, humic acid coated calcium chloride, sulfur-polymer coated urea, diammonium phosphate, granular potassium chloride, zinc sulfate, boric acid, thiodiazole-copper preparation, carbendazim preparation and fenamiphos granular formulation. The release and proportioning of nutrients in the controlled-release special fertilizer for Shandong peanuts are suitable for nutrient conditions and peanut absorption requirements of soil in main peanut production areas in Shandong, and simultaneously, medicaments for preventing and controlling main diseases such as peanut stem rot, root rot, bacterial wilt, root-knot nematode and the like are added. When peanuts are seeded, the disease-preventing type special controlled-release fertilizer for Shandong peanuts is applied for one time, so that labor and time are saved, and disease-preventing and yield-increasing effects are good.

The invention belongs to the technical field of plant protection, and relates to a tobacco-black-shank-resisting and tobacco-bacterial-wilt-resisting bio-control strain Trb3. The bio-control strain is a bacillus subtilis. A method for preparing a microbial agent by using the strain Trb3 comprises the steps that: the strain Trb3 is subjected to oscillation culturing in an LB culture solution for 36-40h under a speed of 180rpm under temperature of 28 DEG C; the obtained material is centrifuged for 10min under a speed of 6000rpm; the material is diluted by using sterile water, such that the microbial agent is prepared, wherein a viable bacteria total concentration in the microbial agent finished product is 1*10<9>-1*10<10>u / ml. The Trb3 strain microbial agent is applied for root dipping before tobacco transplanting or for root irrigating during tobacco transplanting, and for root irrigating after tobacco transplanting and right before disease periods of tobacco black shank and tobacco bacterial wilt. In a greenhouse, a controlling effect of the Trb3 strain microbial agent against tobacco black shank reaches 72.3%, and a controlling effect of the Trb3 strain microbial agent against tobacco bacterial wilt reaches 65.7%. When the Trb3 strain microbial agent is applied in field, a controlling effect of the Trb3 strain microbial agent against black shank and tobacco bacterial wilt is 61.04%.

The invention discloses a special-purpose seedling-raising controlled-release fertilizer for a tobacco seedling-raising plate and a preparation method of the fertilizer, belongs to the technical field of pesticides and solves problem that the existing fertilizer is low in utilization rate and cannot prevent tobacco diseases and insect pests. A weight ratio of N to P to K in a film-coating controlled-release fertilizer is (15-20):(4-10):(6-15); the film-coating controlled-release fertilizer is further added with 0.1%-0.5% of fungicide, wherein the fungicide is prepared from the following materials: tobacco, black false hellebore, garlic, pyrethrum, ailanthus, mandala, uniflower swisscentaury root and weeping willow. The controlled-release fertilizer disclosed by the invention has a long release period, realizes satisfying demands of crops on nutrients such as nitrogen, phosphor, potassium, calcium, sulfur and the like, in the whole growth period by single-dose application. Meanwhile, an occurrence rate of diseases such as tobacco bacterial wilt, black shank and root rot, is greatly reduced, infection of germs in a seedling-raising period is avoided, and occurrence of diseases of stalks which are transplanted in a large field is greatly reduced. And meanwhile, the fertilizer is prevented from adding for many times, and labor cost is greatly lowered.

The invention discloses a method for controlling tobacco bacterial wilt. The tobacco bacterial wilt is controlled by soil improvement, balance of soil nutrient and a tobacco seedling treatment system. The method specifically comprises the following steps of: adding a modifier to a tobacco planting field, then carrying out soil ploughing before winter; fertilizing a base fertilizer to the improved soil; fertilizing the base fertilizer to a hole before transplanting the tobacco seedling; carrying out root dipping treatment by bacteria liquid of paenibacillus polymyxa or bacillus cereus before transplanting the tobacco seedling; carrying out spray treatment by chitosan or brassinolide at the resettling stage; and carrying out root irrigation treatment for 2-3 times by mixed solution of ethylicin or mancozeb at the vigorous growing stage, wherein the interval is 7 days each time. By utilizing the method disclosed by the invention, explosion of the tobacco bacterial wilt can be delayed; the tobacco yield can be increased; the tobacco quality is improved; meanwhile, the acid soil can be improved; and the method can be widely applied to the tobacco planting industry.

The invention relates to microorganism plant vaccine capable of controlling continuous tomato bacterial wilt disease, and belongs to the agricultural intensive production technology. According to the microorganism plant vaccine capable of controlling the tomato bacterial wilt disease, a ralstonia solanacearum sibling bacterium pearson ralstonia eutropha (Ralstoniapickettii) NJQL-A6 is isolated from the rhizosphere of a healthy tomato, and the ralstonia eutropha has a weaking inhibited impact on a bacterial wilt pathogenic bacteria ralstonia eutropha (Ralstonia solanacearum) panel. However, the ralstonia eutropha possesses powerful colonization capability on the tomato rhizosphere and inside a plant, and can inhibit the infection of the ralstonia eutropha to a tomato plant. Experiments show that after the vaccine is irrigated to be applied to soil, the vaccine can breed rapidly so that an advantage group is formed in the soil, and the biocontrol efficiency can be reached up to more than 70% in the soil where the tomato bacterial wilt disease is severe. By inoculating the biocontrol bateria on the tomato plant with the root being infected by the bacterial wilt on the overground part, the further development of the disease can be effectively controlled.

The invention discloses a selective breeding method of a high-resistance tobacco variety and belongs to the technical field of resistive breeding of tobacco. According to the selective breeding method, the tobacco high-yield varieties Yunyan 85, Yunyan 87 and Zhongyan 100 or K326 are taken as recurrent parents, and the cold-resistant variety Honghua Dajinyuan, the drought-resistant variety Zhongyan 14, the brown spot and blackleg resistant variety Zhongyan 86, the root-knot nematode-resistant variety TI1706, the common mosaic virus-resistant variety Taiyan No.7 and the tobacco bacterial wilt-resistant variety D101 as resistant resource parents, and the seven major drought, cold and disease resistant resource genes of the tobacco are pyramided and inserted into the good-quality high-yield tobacco variety with the same genetic background by use of a synchronous backcross and convergent cross method, so that a new high-resistance, high-yield and good-quality tobacco variety, which is highly resistant to 7 drought, cold and disease damage factors and highly consistent with the original recurrent parents in other agricultural and yield traits, is obtained.

The invention relates to bacillus subtilis with a stronger phosphorus decomposing effect and stronger gamma-polyglutamic acid producing capacity. Furthermore, the bacillus subtilis has a better preventing and treating effect on varieties of plant diseases and a plant growth promoting effect. A preservation serial number of the bacillus subtilis JCD-H-16 with phosphorus decomposing, disease preventing and growth promoting effects disclosed by the invention is CGMCC NO.13663, a preservation date is February 16th in 2017, and the bacillus subtilis has multiple effects of decomposing phosphorus, preventing diseases, promoting growth, high producing gamma-polyglutamic acid, increasing yield, improving quality, having a wide antibacterial spectrum and the like. A bacterial strain and a fungicideof the bacillus subtilis disclosed by the invention can effectively prevent and treat powdery mildew, bacterial wilt, gray mold, rice sheath blight disease, pimento anthracnose, damping off and the like; broad-spectrum antibacterial is achieved, and effects are stable and durable; the bacillus subtilis can be applied to developing a bacterial fertilizer and a microbial pesticide preparation integrated with phosphorus decomposing, gamma-polyglutamic acid producing and disease preventing and treating. When the bacterial fertilizer of the bacillus subtilis disclosed by the invention is applied,about one half of a nitrogen fertilizer and a phosphorus fertilizer can be reduced, and an obvious yield increasing effect is further obtained.

The invention relates to a biocontrol bacterial agent. An effective active constituent consists of bacillus subtilis PTS-394. The biocontrol bacterial agent is characterized in that: the biocontrol bacterial agent is aqueous solution or wettable powder. The aqueous solution comprises the following components in percentage by weight: bacillus subtilis PTS-394 with 2 *1,010 cfu / mL of content of bacteria, 0.5 percent of NNO (sodium methylene naphthalene sulfonate), 6 percent of SDBS (sodium dodecyl benzene sulfonate) and 0.1 percent of sodium benzoate. The wettable powder comprises the following components in percentage by weight: 40 percent of bacillus subtilis PTS-394 viable bacterium raw powder with 5 *1,010 cfu / g of content of bacteria, 7 percent of NNO (sodium methylene naphthalene sulfonate), 3 percent of polyethylene glycol, 5 percent of SDBS (sodium dodecyl benzene sulfonate) and 45 percent of light calcium carbonate. The biocontrol bacterial agent is poured into vegetable roots when applied and is used for preventing and controlling vegetable soil-borne diseases such as solanaceous fruit vegetable blight and bacterial wilt mainly; and the content of effective agent components is between 104 and 108 cfu / mL or between 104 and 108 cfu / g after finished product agents are diluted. The biocontrol bacterial agent has the advantages that: the aims of preventing and controlling two kinds of soil-borne diseases by means of one kind of biocontrol bacterial agent are fulfilled by introducing the bacillus subtilis PTS-394 which is safe to people and livestock and has better inhibition effect on the solanaceous fruit vegetable blight and the bacterial wilt; the using amount of chemical agents is reduced, and the agent cost is reduced; and the ecological environment is improved, and the residues of the chemical agents in the vegetables are reduced and the same time.

The invention relates to a Bacillus methylotrophicus Sanju-04 and its application. The above strain is preserved at China Center for Type Culture Collection (CCTCC) in Wuhan University, has a preservation date of May, 16, 2012, and has a preservation number of CCTCC No:M2012169. The Bacillus methylotrophicus Sanju-04 has very good capsicum bacterial wilt and orange anthracnose control efficacies, and has the advantages of stability, no toxic chemical substance residual, no pollution to agricultural products or the environment, high cost performance, and application convenience. The Bacillus methylotrophicus Sanju-04 plays an important role in the control of the bacterial wilt and the anthracnose of plants, and has a wide application prospect.

The invention discloses a Paenibacillus polymyxa strain for preventing and controlling plant bacterial wilt, which is named Paenibacillus polymyxa ZJU0901 through identification and is conserved in China Center for Type Culture Collection (CCTCC) positioned in Wuhan University, Luojia Hill, Wuhan. The conservation date of the strain is July 19th, 2009, and the conservation number of the strain is CCTCC NO:M209157. The strain can produce inhibiting effect on the growth of different biochemical types of strain of ralstonia solanacearum under in vitro conditions and can effectively prevent and control plant bacterial wilt.

The present invention relates to the antagonistic bacteria that are used to prevent and eliminate tobacco bacterial wilt and their microbial organic fertilizer, which belong to the technology for agricultural intensive production. The present invention separates two antagonistic bacteria (Brevibacillus brevis NJL-25 and Bacillus cereus NJL-14) that have remarkable antagonistic action against the pathogens of tobacco bacterial wilt. Microbial organic fertilizer is produced from these antagonistic bacteria and organic compost. Wherein, the content of each of NJL-25 and NJL-14 is above 1×1010 cfu / g, the content of total nitrogen is 4˜5% (above 90% of the nitrogen is organic nitrogen), the content of total nitrogen-phosphorus-kalium nutrient is 6˜10% and the content of organic matter is 30˜35%. As indicated by experiments, after the microbial organic fertilizer is applied into soil, it will enable rapid multiplication of the antagonistic bacteria into a dominant microflora in the soil and achieve more than 97.6% of biocontrol rate of tobacco bacterial wilt in the soil suffering from tobacco bacterial wilt. If the microbial organic fertilizer is applied in the soil without continuous cropping obstacle in a long time, it can prevent the occurrence of the bacterial wilt of continuous cropping tobacco to a great extent.

The invention relates to antagonistic bacteria NJL-14 for preventing and controlling continuous-cropping tobacco bacterial wilt, which belongs to intensive agricultural production technology. According to the invention, a microbial organic fertilizer is produced from two antagonistic bacteria (brevibacillus brevis NJL-25 and bacillus cereus NJL-14) which have a remarkable antagonistic function on pathogenic bacteria of the tobacco bacterial wilt and are obtained by separation, and organic fertilizers, wherein 1 gram of the microbial organic fertilizer contains more than 1*10<10> NJL-25 and 1*10<10> NJL-14, and has the total nitrogen content of 4 to 5 percent (organic nitrogen content is over 90 percent), the total nitrogen-phosphorus-potassium content of 6 to 10 percent and the content of organic matters of 30 to 35 percent. Tests prove that the microbial organic fertilizer, after being applied to soil, can fast multiply the antagonistic bacteria to form a dominant group in the soil, achieve the biological prevention and control rate of over 97.6 percent on the bacterial wilt in the soil with the tobacco bacterial wilt, and prevent the occurrence of the continuous-cropping tobacco bacterial wilt to a great extent after being applied for long to the soil without continuous cropping obstacles.

The invention discloses a biocontrol agent for preventing and curing bacterial wilt and club roots of a plant as well as preparation method and application thereof. The preparation method of the biocontrol agent comprises the following steps: inoculating activated bacillus amyloliquefaciens ZJUB2008 into a culture solution, and fermenting and culturing at 25-45 degree C for 6-120 hours, wherein the culture solution comprises the following components by taking the volume of 1L as the basis: 3-15g of peptone, 0-3g of beef extract, 0-5g of glucose, 0-5g of yeast powder, 0-8g of NaCl, and the balance of water; and the pH value of the culture solution is 5.5-9.0. According to the invention, the bacillus amyloliquefaciens ZJUB2008 is used to prepare the biocontrol agent on / under the appropriate fermentation medium and culture conditions by means of microbial fermentation; in addition, the prepared biocontrol agent has a better prevention effect to different chemotyped ralstonia and plasmodiophora, can remarkably lowering the occurrence rate and severity of the bacterial wilt and the club roots, and is suitable for large-scale production.

The invention relates to an antibiological inoculant, wherein an effective active ingredient of the antibiological inoculant is composed of bacillus amyloliquefaciens FQS38. The antibiological inoculant is characterized by being capable of conducting colonization on the roots of tomatoes, promoting the growth of tomatoes as well as preventing and treating the soil-borne disease of the tomatoes. In addition, the antibiological inoculant can secrete protease, cellulase, siderophores, auximones, gibberellins and other antibacterial and growth promoting ingredients. The antibiological inoculant is an aqueous solution which mainly prepared from the following ingredients in proportion: bacillus amyloliquefaciens FQS38 fermentation liquor (the bacterium content is 1*1010-2*1010cfu / mL) and 0.1 to 0.5% of corrosion remover with the pH value being 5.0 to 6.0. After being diluted, the antibiological inoculant is filled into the roots of the tomatoes in the colonization process so as to prevent and treat the bacterial wilt and the marrow necrosis disease of the tomatoes; and then the subsequent root filling is carried out according to the water-fertilizer rule. After the finished drug is diluted, the effective bacterium content is 106 to 108cfu / mL. The antibiological inoculant has the advantages that the bacillus amyloliquefaciens FQS38 has a good prevention and treatment effect on the bacterial soil-borne disease of the tomatoes, can promote the growing of the tomatoes, has no toxicity and pathogenicity, is safe for people and livestock and is environment-friendly, thereby being possibly applied to field production.

The invention discloses a screening method of a pseudomonas solanacearum bacteriophage resource. The screening method comprises the following steps: S1, taking a soil sample, treating the sample, and filtering by a filter membrane to obtain a filtrate; S2, culturing a pseudomonas solanacearum strain to obtain a pseudomonas solanacearum liquid; S3, co-culturing the filtrate in the S1 and the pseudomonas solanacearum in the S2; and S4, selecting and storing bacteriophage plaque, wherein the used material comprises sterile water, the pseudomonas solanacearum strain, a CPG culture medium, agar powder and low-melting-point agar. The CPG culture medium comprises a CPG solid culture medium and a CPG liquid culture medium. The method can be applied to screening the pseudomonas solanacearum bacteriophage resource from tobacco-planting soil, bacterial wilt soil and sewage and providing and collecting the pseudomonas solanacearum bacteriophage resource for researching prevention and control of tobacco bacterial wilt by using the pseudomonas solanacearum bacteriophage.

The invention discloses a biocontrol actinomycete strain for preventing and controlling tobacco bacterial wilt and application method thereof and belongs to the technical field of micro-organisms. The strain is streptomyces K4, is collected in China General Microbiological Culture Collection Center on March 24, 2014 and has the collection number of CGMCC No. 8949. The strain generates active substances to inhibit the growth of tobacco bacterial wilt and exhibits better prevention and control effects on the tobacco bacterial wilt. The biocontrol actinomycete strain is used for preparing a biocontrol microbial agent and the biocontrol microbial agent can be applied together with rooting water when tobacco seedlings are transplanted; or the biocontrol microbial agent is irrigated into the roots at 4-5 days after the tobacco seedlings are transplanted. The microbial agent can be applied as a bacterial fertilizer prepared by mixing the microbial agent and an organic fertilizer. The strain K4 is obtained from tobacco field rhizosphere soil and is ecologically and harmoniously compatible with soil so that the strain K4 has good application prospects in the biological control of tobacco bacterial wilt.

The invention relates to a dry-cultured paddy rice growth promoting and seedling strengthening agent comprising effective components of, by weight: 60 to 80% of an acidic NPK fertilizer, 0 to 5% of a trace fertilizer, 0.5 to 2.5% of a bacteriostatic preparation, 5 to 15% of an acidity regulating synergist, 0.05 to 0.15% of a chemical regulating preparation, and 5 to 15% of an organic auxiliary agent. When the agent provided by the invention is used on paddy rice directly sowed on dry paddy fields, the seedlings are well-arranged and strong, and are not separated from fertilizers. The roots of the paddy rice are well developed, and the resistances of the paddy rice are high. With the disease preventing function of a special bactericidal agent, manifestations and root causes of diseases can both be treated, and occurrences of rice seedling drooping diseases and bacterial wilts can be effectively prevented. As a result of demonstrational applications of the growth promoting and seedling strengthening agent in 500 acreages of fields in two years, disease preventing and seedling strengthening effects of the agent is substantial. The development and popularization of the agent assist in filling up a gap in domestic researches, and provides a positive effect in the popularization of a paddy rice direct dry sowing technology.

The invention discloses a phage for preventing and controlling soil-borne bacterial wilt, and an application thereof. The phage is a special Ralstonia solanacearum phage NN-742, and the special Ralstonia solanacearum phage NN-742 is preserved in China Center for Type Culture Collection on March 6, 2018 with the preservation number of CCTCC NO: M 2018102, and is named Podoviridae phage. The pathogen-specific phage can efficiently lyse bacteria to make the bacteria dead when used in biological control, and is nontoxic to the environment; and the phage has a strong specificity, does not destroy other normal florae, and can keep soil microbes balanced. The phage can specifically lyse bacterial wilt pathogens, and can be used to prevent and control tomato soil-borne bacterial wilt .

The invention discloses a Brevibacillus laterosporus strain N106-2 of which the collection number is CGMCC No.8482. The strain has strong indoor flat inhibiting actions on multiple plant pathogenic fungi (fussarium wilt of tomato, root rot of chili, gray mold of tomato, Rhizoctonia solani, black spot of cabbage, anthracnose of pear and black rot of pear) and plant pathogenic bacteria (bacterial wilt of tomato, fire blight of pear and streak of rice); and the strain can secrete proteinase, cellulase and surfactant antimicrobial related substances with hemolytic activity. The Brevibacillus laterosporus can be prepared into a biocontrol aqua; the biocontrol aqua has certain preventing and treating effects on fussarium wilt of tomato, fusarium rot of chili, anthracnose of pome and eyespot of pome. The Brevibacillus laterosporus N106-2 has the advantages of no toxicity, no pathogenicity and high safety for human and livestock, is environment-friendly, is a useful resource for development of microbial pesticides, and has potential application prospects.