279 results about "Glucanase" patented technology

The invention discloses bacillus velezensis YB-1652, a biocontrol microbial inoculum and application of the biocontrol microbial inoculum, and aims to solve the technical problem of lack of effective biological control measures for continuous accumulation of peanut phenolic acid autotoxic substances and pathogens in continuous cropping. According to the application, a bacillus velezensis YB-1652 strain is separated and screened from rhizosphere soil of continuous cropping peanuts, and the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.36152. The strain has the capability of efficiently degrading phenolic acid compounds, relieves continuous cropping obstacles and improves the soil environment; the compound has a relatively strong inhibition effect on southern blight and root rot of peanuts, and the occurrence rate of soil-borne diseases is reduced; meanwhile, peanut growth can be promoted; the bacillus amyloliquefaciens has siderophore, cellulase, beta-glucanase, amylase power and protease production capacity. The YB-1652 strain and the preparation thereof can provide technical support for restoration of peanut continuous cropping obstacles, provide bacterial source guarantee for prevention and treatment of peanut soil-borne diseases, replace or reduce use of chemical pesticides and improve food and ecological environment safety, and have good economic and social benefits.

The invention provides a Kitasatospora xanthophycea JY01 strain and application thereof, the strain is separated from intestinal tracts of spring silkworms, has good disease-resistant and growth-promoting effects, and is specifically expressed in that cellulase, beta-1, 3-glucanase, siderophore and auxin IAA can be secreted; pot experiments show that the biocontrol efficiency of the microbial inoculum to wheat stalk rot is 57.90%, the biocontrol efficiency of the microbial inoculum to corn stalk rot is 46.41%, and the plant height and fresh weight are both significantly increased. The microbial agent is expected to be applied to preparation of biological bacterial fertilizer for effectively preventing and controlling soil-borne fungal diseases.

The invention discloses an endoglucanase truncated mutant and an application of the endoglucanase truncated mutant. According to the invention, based on endoglucanase BsEGL from bacillus subtilis DLG, a truncated mutant is constructed by deleting a carbohydrate binding domain (CBM) of the endoglucanase BsEGL, and the truncated mutant is named as BsEGL [delta] CBM. Compared with BsEGL, the truncated mutant BsEGL delta CBM has the advantages that the thermal stability, the pH stability, the organic solvent tolerance and the like are remarkably improved, and the activity of degrading phosphoric acid expanded cellulose and corncob xylan is obtained. In addition, the enzymatic activity of the truncated mutant BsEGL delta CBM is remarkably improved by 6.41%, and the catalytic efficiency is remarkably improved. Due to the characteristics, the mutant can be suitable for industrial processes such as feed processing, papermaking, biological energy sources, spinning and food brewing, and is particularly suitable for enzyme catalytic reaction under extreme environments such as high temperature and low pH. The invention provides an enzyme preparation tool with excellent performance for efficient degradation of cellulose biomass, and has important application value.

The invention discloses a method for extracting eucommia ulmoides with an intestinal repair function, and relates to the technical field of natural product extraction.The method comprises the steps that a fresh eucommia ulmoides raw material is subjected to low-temperature drying and smashing, a polysaccharide water extract and filter residues are obtained through water extraction, and then the flavone residual quantity in the polysaccharide water extract is synchronously detected; carrying out enzymolysis on the polysaccharide water extract through beta-glucanase to obtain a small molecular polysaccharide solution, and carrying out alcohol extraction on filter residues to obtain a flavone alcohol extract; the preparation method comprises the following steps: mixing the two components according to a ratio, inoculating compound probiotics for anaerobic fermentation, regulating according to the residual quantity of flavone, regulating an alcohol extraction process by using short-chain fatty acid generated by fermentation when the residual quantity is high, supplementing flavone extract in an enzymolysis system when the residual quantity is low, and finally removing impurities, purifying, adapting intestinal tracts and drying to obtain the product. According to the method, regulation and control operation is correspondingly executed by detecting the residual quantity of flavone in the polysaccharide water extract, so that self-supply of a regulation and control reagent and high efficiency of active ingredients are realized, the conversion rate of small molecular polysaccharide and flavone aglycone is increased, and the intestinal tract tolerance of the product is optimized.

The invention discloses an efficient extraction method of large-molecular-weight astragalus polysaccharide, which belongs to the technical field of plant extraction, and comprises the following steps: cleaning astragalus, filtering, and crushing; the preparation method comprises the following steps: mixing a beta-1, 3-glucanase aqueous solution and trehalose ethylene diamine, and stirring for 10-30 minutes to obtain an enzyme protection system; soaking the radix astragali powder in a citric acid solution, adding an enzyme protection system, applying a bipolar pulsed electric field, and reacting at 40-60 DEG C for 30-40 minutes; adjusting the concentration of the ethanol to 40-50%, standing, centrifuging and collecting crude astragalus polysaccharide; purifying, decolorizing, concentrating, freeze-drying and crushing the crude astragalus polysaccharide to obtain refined astragalus polysaccharide; the mass ratio of the trehalose ethylene diamine to the beta-1, 3-glucanase is (15: 1) to (30: 1). The enzyme protection system maintains the activity of the enzyme, the enzyme and the pulsed electric field act synergistically, the extraction time is shortened, and the extraction rate is increased while the astragalus polysaccharide with large molecular weight is obtained.

The invention relates to the technical field of microorganisms, and particularly provides application of bacillus amyloliquefaciens BA-89 in crop growth promotion and verticillium dahliae antagonism. The strain is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.36498. The strain can secrete multiple extracellular enzymes (including protease, amylase, cellulase and glucanase), has the growth promoting characteristics of producing ammonia, dissolving potassium and the like, and can tolerate high temperature and ultraviolet stress; the compound has a remarkable antagonistic effect on various plant pathogenic bacteria (such as verticillium dahliae, fusarium oxysporum and phytophthora capsici); by inoculating the strain on cotton, the plant growth can be obviously promoted, the plant height, stem diameter, root length, leaf area and biomass are improved, and meanwhile, the disease index of verticillium wilt and the colonization amount of pathogenic bacteria at the root are effectively reduced. The strain disclosed by the invention can be used as a biological fertilizer and a biological pesticide, is used for cotton cultivation and disease prevention and control, and has a wide application prospect.

The invention discloses a method and a system for producing high-purity medium-chain fatty acid (MCFA) based on waste molasses two-stage regulation fermentation. Comprising the following steps: (1) diluting waste molasses, then adding a compound enzyme agent of pectinase, beta-glucanase and cellulase, and coupling microwave to assist treatment; (2) inoculating the pretreated waste molasses with a composite flora containing a clostridium kluyveri mutant strain and a Megasphaera elsdeni engineering bacterium, and carrying out a fermentation treatment on the waste molasses, wherein the composite flora contains the clostridium kluyveri mutant strain and the Megasphaera elsdeni engineering bacterium; (3) sequentially performing two-stage dynamic regulation and control fermentation on the waste molasses inoculated with the composite flora through a pH-stat control strategy to realize efficient conversion from butyric acid to hexanoic acid / octanoic acid; and (4) a hydrophobic hollow fiber membrane module is adopted to realize on-line removal of the product, and the inhibition effect of the product is relieved. According to the technology, the yield of the MCFA produced from the waste molasses reaches 0.45 g / g COD, the cost is reduced by 68% compared with that of a traditional substrate, and a new way is provided for recycling of sugar manufacturing waste.

The invention discloses a low-temperature-resistant and drought-resistant straw degrading bacterium and application thereof. The low-temperature-resistant and drought-resistant straw degrading bacterium is obtained through screening, is stenotrophomonas maltophilia GF-Y18, has good capacity of producing exon-beta-1, 4-glucanase, laccase and lignin peroxidase, can effectively promote straw decomposition, has certain low-temperature-resistant and drought-resistant capacity, can be used for degrading straw, and can be applied to the field of straw degradation. The strain and the fungicide thereof can be used for degrading lignin and / or cellulose and / or straw in a low-temperature drought environment.

The invention discloses a method for generating rare ginsenoside Rh2, which comprises the following steps: adding common glycol type ginsenoside into aspergillus brazilis fermentation liquor for fermentation, and sterilizing after fermentation to obtain a fermentation product; and carrying out enzymolysis by using beta-glucanase and pectinase, and carrying out enzyme deactivation after the enzymolysis is finished, so as to obtain the rare ginsenoside Rh2. The invention also provides the rare ginsenoside Rh2 prepared by the method and application of the rare ginsenoside Rh2. The common glycol type ginsenoside is efficiently and quickly converted into the rare ginsenoside Rh2 through the synergistic interaction of the aspergillus brazilis, the beta-glucanase and the pectinase; the rare ginsenoside Rh2 has the advantages of being simple in preparation method, easy to implement, low in cost, environmentally friendly, free of pollution and the like, and the generated rare ginsenoside Rh2 shows excellent skin care and repairing effects and has wide market prospects when applied to cosmetics.

The present invention relates to the technical field of bacterial enzyme fermentation, and provides a bacterial enzyme synergistic fermentation method for removing antinutritional factors in soybean meal, the method comprises the following steps: S1, soybean meal pretreatment: soybean meal, water and an auxiliary agent are mixed and then subjected to steam explosion treatment, and the auxiliary agent comprises citric acid, a hydrogen peroxide solution and polyphenol; s2, bacterial enzyme fermentation: mixing the pretreated soybean meal with molasses and water to obtain a raw material, adding a complex microbial inoculant and a complex enzyme, uniformly stirring, and fermenting; the complex microbial inoculants comprise saccharomycetes, bacillus subtilis, lactobacillus rhamnosus and pediococcus pentosaceus; the compound enzyme comprises acid protease, pectinase, phytase, glucanase, alpha-galactosidase and keratinase; and S3, after fermentation is finished, drying the materials to obtain the fermented soybean meal. According to the fermentation method, the glycinin degradation rate can reach 79% or above, the acid soluble protein content is increased to 26% or above, the anti-nutritional factor content is remarkably reduced, and the nutritional value is greatly increased.

The invention relates to the technical field of agriculture, and discloses a synergistic technology for repairing soil and promoting plant stem cells by using modified amino active groups. Comprising the following steps: modifying an amino acid compound, namely modifying active groups in amino acid and fulvic acid, so that the active groups have a synergistic effect with cellulase, hemicellulase, urease, phosphatase, chitinase, beta-glucanase, superoxide dismutase, peroxidase and catalase; through the strong chelation of the modified amino active groups and heavy metal ions in soil, the bio-availability and mobility of heavy metals can be effectively reduced, for example, the chelation removal rate of cadmium, lead and other heavy metals can reach 40% or above, the heavy metal contaminated soil is repaired, the soil structure is improved, the content of organic matters in the soil is increased, and the pH value of the soil is adjusted; the acidified or alkalized soil is gradually restored to a pH range suitable for plant growth, and the water and fertilizer retention capability of the soil is improved.

The invention discloses an incision beta-1, 3-glucanase mutant with improved enzyme activity and thermal stability, and belongs to the technical field of gene engineering and enzyme engineering. According to the invention, a site-directed mutagenesis technology is utilized to carry out molecular modification on wild type beta-1, 3-glucanase, and a series of mutants S22F, N226D and N187S are obtained. Compared with a wild enzyme, the beta-1, 3-glucanase mutant constructed by the invention has the advantages that the enzyme activity is improved by 1.04-1.81 times, the Tm value is improved by 0.97-1.19 DEG C, and the half-life period at 70 DEG C is prolonged to 1.08-1.28 times. According to the invention, the enzyme activity and the thermal stability of the wild beta-1, 3-glucanase are improved at the same time, and better use conditions are created for the enzyme in practical application.

The invention relates to the technical field of cyanobacterial bloom control in water, and particularly discloses a compound enzyme-bacterium synergistic preparation for cyanobacterial bloom control, a preparation method and a use method. The preparation comprises the following components in percentage by mass: compound enzymes (10-20% of cellulase, 8-12% of beta-1, 3-glucanase and 3-8% of lipase), functional bacteria (10-20% of bacillus subtilis, 8-15% of trichoderma harzianum and 5-10% of bacillus amyloliquefaciens), an enzyme stabilizer (3-8% of trehalose), an adsorption carrier (15-25% of Fe < 3 + > modified charcoal and 8-15% of diatomite) and auxiliary components. The preparation method comprises the steps of carrier modification, solid fermentation and microcapsule embedding. During use, 3-20kg / 1000m < 3 > of water is added for the first time, 2-10kg / 1000m < 3 > of water is added every 7-14 days in the maintenance stage, and synchronous aeration is performed. Through the synergistic effect of beta-1, 3-glucanase and trichoderma harzianum, the algicidal rate is 92%, and the spore germination inhibition rate is 82%; the Fe < 3 + > modified charcoal improves the stability of the enzyme in an alkaline environment (80% of activity is retained when the pH value is 9); the method is suitable for eutrophic water with chlorophyll a greater than or equal to 30 [mu] g / L, and has no chemical residue risk.

The invention discloses a leontopodium alpinum callus extract as well as a preparation method and application thereof, and belongs to the technical field of plant active ingredient extraction. The leontopodium alpinum callus extract is prepared by the following steps: mixing leontopodium alpinum callus freeze-dried powder with a buffer solution, adding cellulase, pectinase and beta-glucanase, uniformly mixing, reacting to obtain primary enzymolysis slurry, adjusting the primary enzymolysis slurry to be neutral, adding phospholipase A2, stirring, reacting to obtain deep enzymatic hydrolysate, filtering, washing, and drying to obtain the leontopodium alpinum callus extract. Transferring into an extraction kettle, adding an entrainer, and extracting to obtain a leontopodium alpinum extract; dissolving soybean phospholipid and cholesterol in ethanol to obtain an organic phase; dispersing the leontopodium alpinum extract in a buffer solution to obtain an active matter water phase; and injecting the organic phase into the active matter water phase, stirring, carrying out reduced pressure distillation, and homogenizing. The preparation method provided by the invention can efficiently and completely obtain the active component, and the nano-liposome formed by phospholipid molecules can effectively improve the stability of the active component.

The invention relates to the technical field of microorganism application, in particular to low-nucleic-acid yeast protein as well as a preparation method and application thereof. The preparation method of the low-nucleic-acid yeast protein comprises the following steps: (1) carrying out inactivation treatment on high-protein yeast; (2) carrying out enzymolysis on the inactivated yeast by adopting neutral protease, glucanase, mannase and first nuclease, and after enzyme deactivation, separating and taking a heavy phase to obtain crude yeast protein; (3) performing high-pressure homogenization treatment on the crude yeast protein; and (4) carrying out enzymolysis on the crude yeast protein subjected to high-pressure homogenization treatment by adopting second nuclease and lipase, and after enzyme deactivation, separating and taking a heavy phase to obtain the low-nucleic-acid yeast protein. The yeast protein with the nucleic acid content of 1.0% or below is obtained by taking the high-protein yeast as a raw material through inactivation, compound enzyme enzymolysis, high-pressure homogenization treatment and secondary enzymolysis, is neutral and pure in taste, and can be widely applied to the fields of nutrition, health and food such as protein supplementation and protein substitution.

A method for immobilizing beta-glucanase based on a rice husk-sodium alginate composite carrier belongs to the field of enzyme engineering and biological materials, and comprises the following steps: 1) crushing and sieving rice husks; 2) mixing rice husks and sodium alginate according to a mass ratio of 1: 3-1: 6, and performing ultrasonic dispersion and magnetic stirring to form a carrier solution; (3) adding beta-glucanase into the carrier solution, and reacting for 35-50 minutes at the temperature of 30-35 DEG C; and 4) dropwise adding the enzyme-carrier mixed solution into a calcium chloride solution for curing and forming, and washing and drying to obtain the immobilized enzyme particles. By optimizing the ratio of the composite carrier, when the mass ratio of the rice husks to the sodium alginate is 1: 4, the catalytic activity of the prepared immobilized enzyme under unit mass is obviously higher than that of other ratio groups. According to the method, agricultural waste rice hulls are used as main raw materials, the cost is low, the process is simple, the prepared immobilized enzyme is high in activity, and a new way is provided for industrial application of beta-glucanase.

The invention belongs to the technical field of radix gentianae extracts, and discloses a preparation method and application of a radix gentianae extract, and the preparation method of the radix gentianae extract comprises the following steps: S1, adding radix gentianae powder and chitosan-nano calcium carbonate composite microspheres into a buffer solution, and uniformly mixing to obtain a suspension 1; s2, firstly adding cellulase into the suspension 1, then adding beta-glucanase and flavourzyme, and after enzymolysis is finished, obtaining a suspension 2; and S3, adding the suspension 2 into a PEG-400 / ionic liquid ([BMIM] BF) / water extraction medium, carrying out primary extraction at 20-30 DEG C and secondary extraction at 55-65 DEG C to obtain a crude extract, and carrying out post-treatment purification to obtain the radix gentianae extract. The radix gentianae extract prepared by the preparation method disclosed by the invention is high in content of effective components, high in stability of the effective components and low in impurity content.

The present application relates to a glucanase variant.

The present invention relates to polypeptides having endo-1,3-α-glucanase activity, polynucleotides encoding the polypeptides, nucleic acid constructs, vectors, host cells comprising the polynucleotides, methods of producing the polypeptides, as well as oral care compositions comprising the polypeptides.

The invention discloses breeding of a pichia kluyverovii strain with high yield of beta-lyase and application of the pichia kluyverovii strain in beer fermentation, and belongs to the technical field of bioengineering. The pichia kluyveri B21 is preserved in the China General Microbiological Culture Collection Center, and the preservation number of the pichia kluyveri B21 is CGMCC (China General Microbiological Culture Collection Center) No.34028. The strain can produce high-yield beta-lyase, and the beta-lyase is catalyzed to produce volatile mercaptan and endows beer with fruity flavor. The contents of ethyl acetate, isoamyl acetate, isobutyl acetate, ethyl nonanoate, ethyl caprate, phenethyl acetate, linalool and nerol generated by the pichia kluyveri B21 in a hops simulated culture medium are relatively high, and the volatile substances can increase the fruit fragrance and flower fragrance of beer and enrich the flavor of the beer. The pichia kluyveri B21 is expected to be added into production of other food and beverages so as to enrich flavor substance components.

The invention discloses a composite nut polypeptide solid beverage and a preparation method thereof. The health-care food is prepared from the following components in parts by weight: 20 to 40 parts of walnuts, 15 to 30 parts of cashew nuts, 10 to 25 parts of almonds, 12 to 28 parts of almonds, 8 to 20 parts of hazelnuts, 0.3 to 0.8 part of compound protease, 0.05 to 0.15 part of beta-glucanase, 10 to 20 parts of isomaltooligosacharide, 5 to 15 parts of resistant dextrin, 8 to 18 parts of maltodextrin, 0.1 to 0.3 part of silicon dioxide, 0.05 to 0.15 part of vitamin C, 0.02 to 0.08 part of gamma-aminobutyric acid, 0.03 to 0.1 part of taurine and 2 to 5 parts of natural strawberry powder. And 0.05 to 0.15 part of natural vanilla essence. The invention discloses and provides a composite nut polypeptide solid beverage and a preparation method thereof, a composite enzymolysis technology is adopted, nut protein is hydrolyzed into small-molecule polypeptides with the average molecular weight lower than 1000Da, and the polypeptides have high biological activity and bioavailability.

The application discloses an endoglucanase mutant with acid resistance and application thereof, and belongs to the technical field of genetic engineering and enzyme engineering. The endoglucanase tCel5A is subjected to site-specific saturation mutation by PCR amplification, and n variants S45D / T55D / T59D and T88H / W255H are obtained through screening. Enzyme activity determination results show that the endoglucanase variants S45D / T55D / T59D and T88H / W255H have enzyme activities of 8828.7 U / mg prot and 5206.7 U / mg prot respectively under acidic conditions, and the degradation rates of the endoglucanase variants S45D / T55D / T59D and T88H / W255H on distillers' grains are 53.53% and 50.47% respectively under a solid-liquid ratio of 1:30, so that the endoglucanase variants have strong acid resistance and degradation capacity on plant cellulose, and have great application value in agriculture and brewing.

The invention belongs to the technical field of packaging materials. The invention relates to an adhesive, in particular to a PVA adhesive tape adhesive and a preparation method thereof. The preparation method comprises the following specific preparation steps: mixing guar gum and water according to a mass ratio of 1: (15-20) for swelling, adding endo-beta-glucanase accounting for 0.3-0.5% of the mass of the guar gum, carrying out ultrasonic enzymolysis reaction for 30-45 minutes under the conditions that the pH is 5.0-5.2, the temperature is 48-52 DEG C and the ultrasonic frequency is 50-60 kHz, heating for enzyme deactivation, and cooling to obtain an activated vegetable gum solution; uniformly stirring and mixing a PVA (Polyvinyl Alcohol) solution and the activated vegetable glue solution, adding a thickening agent solution, continuously stirring and mixing uniformly, adding a composite modifier and potassium persulfate, carrying out heat preservation curing reaction for 2-4 hours at the temperature of 40-45 DEG C, aging for 24-36 hours, and discharging to obtain a mixed glue solution; and coating the mixed adhesive solution, and drying to obtain the PVA adhesive tape adhesive.

The invention provides a preparation method, a formula and application of oat thick slurry rich in beta-glucan, and belongs to the technical field of food processing. The preparation method comprises the steps that S1, oat is ground into powder through a turbulence ultrafine grinder, and the particle size of the ground oat powder is 10-20 m; s2, mixing the oat powder with water, adding a complex enzyme preparation, stirring and preserving heat to obtain a first enzymatic hydrolysate, the complex enzyme preparation comprising protease and cellulase; s3, adding beta-glucanase into the first enzymatic hydrolysate, and performing enzymolysis to obtain a second enzymatic hydrolysate; s4, sequentially performing enzyme deactivation treatment, sieving treatment, homogenizing treatment, sterilization treatment and sterile filling on the second enzymatic hydrolysate to obtain the oat thick pulp rich in beta-glucan. According to the method disclosed by the invention, the dissolution rate of the beta-glucan in the prepared oat thick slurry rich in the beta-glucan can be effectively improved by crushing the oat, adding the compound enzyme preparation and adding the beta-glucanase.

Provided are a recombinant Escherichia coli with improved ability to secrete extracellular endoglucanase (BcsZ) and a construction method therefor. The recombinant Escherichia coli is constructed by using Escherichia coli JBZ-DH5α as a starting strain and inserting a membrane protein coding gene. The membrane protein gene is one of the following coding genes: a coding gene of zinc transporter permease znuB, a coding gene of DUF1449 family inner membrane protein ylaC, and a coding gene of UPF0410 family inner membrane protein ymgE.

The invention provides high-activity crataegus pinnatifida fruit vinegar and a preparation method thereof.The preparation method comprises the steps that crataegus pinnatifida fruits are juiced, then a composite chelating agent composed of polyglucuronic acid and monopotassium phosphate is added for chelating treatment, and a polysaccharide-metal ion cross-linked network is removed; then adding endo-beta-1, 4-glucanase to carry out linear polysaccharide directional enzymolysis, so that the viscosity of the fruit juice is obviously reduced; after the components are adjusted, double modified yeast beta-glucan microspheres are added, yeast is inoculated for alcoholic fermentation, the microspheres are subjected to double grafting modification through galactooligosaccharide and theaflavin, and active components in the crabapple can be directionally captured and stabilized through hydrophilic and hydrophobic effects respectively; after alcohol fermentation is finished, acetic bacteria are inoculated for acetic fermentation, and finally, filtering and sterilization are performed. According to the method disclosed by the invention, by virtue of a synergistic strategy of breaking a network firstly, then carrying out enzymolysis debonding and then carrying out microsphere loading, the fermentation mass transfer efficiency is effectively improved, and meanwhile, the retention rate of functional components such as polyphenol and saponin in the fruit vinegar is increased.

A process for upcycling spent coffee grounds into an antioxidant dietary fiber involves a microwave assisted extraction of the spent coffee grounds in water at a temperature up to 200° C. for a time of at least 5 min, followed by an enzymatic assisted extraction of the spent coffee grounds in an aqueous solution including an enzyme having endo-beta-glucanase activity and / or an enzyme having cellulase activity. The process further involves recovering soluble matter. Prior to the microwave assisted extraction step, the spent coffee grounds are subjected to a pulsed electric field treatment or a high-pressure extraction or both.

The present invention belongs to the field of genetic engineering and enzyme engineering, and relates to a chassis strain lacking the bglS gene and its application. The present invention provides a genetically engineered chassis strain of Bacillus subtilis, wherein the chassis strain is constructed by deleting the glucanase encoding gene on the Bacillus subtilis host genome. bglS The described chassis bacteria can efficiently express alkaline protease, aminopeptidase, mesophilic α-amylase, and thermostable α-amylase. This method is also suitable for increasing the expression of other exocrine proteins, laying the foundation for efficient expression of heterologous proteins and promoting the efficient expression and industrial production of alkaline protease, aminopeptidase, mesophilic α-amylase, and thermostable α-amylase.

The invention provides a multienzyme system for preparing cellobiose as well as a preparation method and application of cellobiose, and belongs to the technical field of bioengineering. The multi-enzyme system disclosed by the invention is prepared from endoglucanase, cellobiose hydrolase and lysed polysaccharide monooxygenase, wherein the endoglucanase is EG5C-1m, the cellobiose hydrolase is TeCel7A T393K, and the lysed polysaccharide monooxygenase is LsAA9A. According to the method disclosed by the invention, a multi-enzyme system for accurately degrading cellulose into cellobiose is constructed by screening high-efficiency endoglucanase, cellobiose hydrolase and lysed polysaccharide monooxygenase. On the basis, the optimal reaction condition of a multi-enzyme system is determined, a method is provided for efficient generation of cellobiose from cellulose, and a basis is provided for subsequent biomass conversion of high-value products.