436 results about "Glucanase" patented technology

The invention discloses Trichoderma longibrachiatum NT241 and application thereof, the microbiological classification name of the Trichoderma longibrachiatum NT241 is Trichoderma longibrachiatum NT241, the Latin literature name of the Trichoderma longibrachiatum NT241 is Trichoderma longibrachiatum NT241, the preservation number of the Trichoderma longibrachiatum NT241 is CGMCC (China General Microbiological Culture Collection Center) No.41139, and the preservation address of the Trichoderma longibrachiatum NT241 is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The trichoderma longibrachiatum NT241 disclosed by the invention grows quickly, the generated endo-beta-glucanase and manganese peroxidase are relatively high in activity, the degradation of straws of crops such as oilseed rape and wheat is promoted by adding the liquid fungicide, and an efficient microbial resource can be provided for straw returning utilization.

The invention discloses bacillus velezensis YB-1652, a biocontrol microbial inoculum and application of the biocontrol microbial inoculum, and aims to solve the technical problem of lack of effective biological control measures for continuous accumulation of peanut phenolic acid autotoxic substances and pathogens in continuous cropping. According to the application, a bacillus velezensis YB-1652 strain is separated and screened from rhizosphere soil of continuous cropping peanuts, and the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.36152. The strain has the capability of efficiently degrading phenolic acid compounds, relieves continuous cropping obstacles and improves the soil environment; the compound has a relatively strong inhibition effect on southern blight and root rot of peanuts, and the occurrence rate of soil-borne diseases is reduced; meanwhile, peanut growth can be promoted; the bacillus amyloliquefaciens has siderophore, cellulase, beta-glucanase, amylase power and protease production capacity. The YB-1652 strain and the preparation thereof can provide technical support for restoration of peanut continuous cropping obstacles, provide bacterial source guarantee for prevention and treatment of peanut soil-borne diseases, replace or reduce use of chemical pesticides and improve food and ecological environment safety, and have good economic and social benefits.

The invention provides a Kitasatospora xanthophycea JY01 strain and application thereof, the strain is separated from intestinal tracts of spring silkworms, has good disease-resistant and growth-promoting effects, and is specifically expressed in that cellulase, beta-1, 3-glucanase, siderophore and auxin IAA can be secreted; pot experiments show that the biocontrol efficiency of the microbial inoculum to wheat stalk rot is 57.90%, the biocontrol efficiency of the microbial inoculum to corn stalk rot is 46.41%, and the plant height and fresh weight are both significantly increased. The microbial agent is expected to be applied to preparation of biological bacterial fertilizer for effectively preventing and controlling soil-borne fungal diseases.

The present disclosure relates to a process for preparing an upcycled material from a dry raw material with a moisture content of 0-30% w / w comprising lignocellulose and derived from spent cereal and / or pseudocereal grain and / or food waste or lignocellulosic biomass or a mixture thereof, the process comprising: a) a pre-treatment comprising the steps of: i) grinding and / or pulverizing the raw material to obtain a powder capable of passing through a sieve having a mesh size of 10-1000 μm and ii) performing autohydrolysis, followed by b) enzymatic hydrolysis of the of powder obtained from step ii), wherein the enzymatic hydrolysis comprises adding an enzyme mixture comprising at least a glucanase and a xylanase, and c) a post-treatment comprising the step of grinding and / or pulverizing the enzymatically hydrolysed mixture obtained from step b) to obtain the upcycled material in the form of a flour-like product.

The invention relates to the technical field of microorganisms and bio-organic fertilizers, and particularly discloses a bio-organic fertilizer for preventing and treating rhizome diseases of horseradish and application of the bio-organic fertilizer, the bio-organic fertilizer comprises the following components: component A: a bacillus velezensis microbial inoculum with the preservation number of CCTCC NO: M 2022923 and a trichoderma yunnanense microbial inoculum with the preservation number of CCTCC NO: M 2025645; the component B is prepared from soybean meal, vinegar residues, charcoal, wollastonite powder and allicin. The biological organic fertilizer disclosed by the invention can be used for increasing the content of growth hormones such as salicylic acid (SA), jasmonic acid (JA) and ethylene (ET) and phytoalexin as well as the activity of chitinase and beta-1, 3-glucanase of a wasabi root system, and has a relatively good prevention and treatment effect on a black heart disease caused by infection of phoma wabi Yokog and a soft rot disease caused by infection of pectobacterium carotovorum subsp. Carotovorum (Pcc). The biological organic fertilizer disclosed by the invention has a relatively good prevention and treatment effect on the soft rot disease caused by infection of the phoma wabi Yokog and a relatively good prevention and treatment effect on the soft rot disease caused by infection of the phoma wabi subsp. Carotovorum (Pcc).

The present invention provides L-β-galactoglucan, a preparation method therefor, and a use thereof. The polysaccharide is sinistrin, is composed of β-D-glucose and β-D-galactose, and has a molecular weight of not less than 5000 Da. The preparation method for the polysaccharide comprises: inoculating Agrobacterium FN01 in a culture solution for fermentation to obtain a fermentation broth; mixing the fermentation broth with alcohol, and carrying out precipitation and drying to obtain a crude polysaccharide product; and then carrying out purification to obtain a purified polysaccharide product, or carrying out enzymolysis on the fermentation broth by using glucanase, and then carrying out filtration, ultrafiltration concentration, and spray-drying to obtain a purified polysaccharide product. The polysaccharide has the effects of treating and / or preventing liver injury, resisting aging, decreasing blood sugar, repairing tissues and the like, and is a multipurpose polysaccharide.

The invention discloses an endoglucanase mutant with acid resistance and application of the endoglucanase mutant, and belongs to the technical field of gene engineering and enzyme engineering. According to the present invention, PCR amplification is adopted to carry out site-specific saturation mutation on endoglucanase tCel5A, and n variants S45D / T55D / T59D and T88H / W255H are constructed and screened; the enzyme activity determination result shows that the enzyme activities of the endoglucanase variants S45D / T55D / T59D and T88H / W255H under the acidic condition are 8828.7 U / mg prot and 5206.7 U / mg prot respectively, and the degradation rates of the endoglucanase variants S45D / T55D / T59D and T88H / W255H to vinasse under the solid-to-liquid ratio of 1: 30 are 53.53% and 50.47% respectively, so that the endoglucanase variants S45D / T55D / T59D and T88H / W255H have high acid resistance and plant cellulose degradation capacity and have great application value in the aspects of agriculture and brewing.

The invention discloses a high-activity endoglucanase expression optimized strain and application thereof, and belongs to the technical field of gene engineering and enzyme engineering. According to the present invention, PCR amplification and homologous recombination are adopted to carry out strain construction and expression optimization on endoglucanase tCel5A, and screening is performed to obtain the sAOX1-pSUC2-tCel5A strain; an enzymatic property research finds that the recombinant pPICZA-tCel5A has the optimal temperature of 50 DEG C and the optimal reaction pH of 4.5, and has good thermal stability and low pH stability. The enzyme activity determination result shows that the enzyme activity of the sAOX1-pSUC2-tCel5A after expression optimization is 4632.81 U / mg prot, which indicates that the tCel5A after expression optimization can efficiently degrade cellulose, and has a great application prospect in the fields of agriculture, brewing industry and the like.

The invention discloses difunctional glucanase for hydrolyzing agricultural and sideline products and application, and belongs to the technical field of biology. The difunctional glucanase is named as RuCel071, and the amino acid sequence of the difunctional glucanase is as shown in SEQ ID NO. 3. The novel cellulase RuCe071 is developed on the basis of a rumen microorganism metagenome resource mining technology, has the activity of simultaneously hydrolyzing cellulose and xylan, can be used for degrading agricultural and sideline products, reducing accumulation of the agricultural and sideline products and environmental pollution, lowering the treatment cost and improving the degradation efficiency, and has the advantages of being simple in preparation process, low in cost and high in application value. The utilization rate and the economic benefit of agricultural and sideline products are favorably improved.

The invention relates to beta-1, 4-glucanase and application thereof, and belongs to the technical field of biology. A novel beta-1, 4-glucanase is screened and identified in Pleurotus tuber-regium, and the beta-1, 4-glucanase has wide substrate specificity, can specifically hydrolyze beta-glucan (such as sodium carboxymethyl cellulose) containing beta-1, 4 glucosidic bonds, and can hydrolyze beta-glucan (such as sodium carboxymethyl cellulose) containing beta-1, 3-1, 4-glucanase) containing beta-1, 3-1, 4-glucanase. The beta-1, 4-glucanase disclosed by the invention can be used for preparing beta-glucan (such as barley beta-glucan and oat beta-glucan) containing beta-1, 3-1, 4 glucosidic bonds, so that the beta-1, 4-glucanase disclosed by the invention has good enzyme activity on the beta-glucan containing the beta-1, 4 glucosidic bonds and the beta-glucan containing the beta-1, 3-1, 4 glucosidic bonds. Besides, the enzyme activity of the beta-1, 4-glucanase disclosed by the invention can be kept at 70% or above under the condition that the pH value is 4.0-7.0, the enzyme activity of the beta-1, 4-glucanase can be kept at 88% or above under the temperature of 40 DEG C, and the beta-1, 4-glucanase has good stability.

The invention discloses an endoglucanase truncated mutant and an application of the endoglucanase truncated mutant. According to the invention, based on endoglucanase BsEGL from bacillus subtilis DLG, a truncated mutant is constructed by deleting a carbohydrate binding domain (CBM) of the endoglucanase BsEGL, and the truncated mutant is named as BsEGL [delta] CBM. Compared with BsEGL, the truncated mutant BsEGL delta CBM has the advantages that the thermal stability, the pH stability, the organic solvent tolerance and the like are remarkably improved, and the activity of degrading phosphoric acid expanded cellulose and corncob xylan is obtained. In addition, the enzymatic activity of the truncated mutant BsEGL delta CBM is remarkably improved by 6.41%, and the catalytic efficiency is remarkably improved. Due to the characteristics, the mutant can be suitable for industrial processes such as feed processing, papermaking, biological energy sources, spinning and food brewing, and is particularly suitable for enzyme catalytic reaction under extreme environments such as high temperature and low pH. The invention provides an enzyme preparation tool with excellent performance for efficient degradation of cellulose biomass, and has important application value.

The invention discloses a method for extracting eucommia ulmoides with an intestinal repair function, and relates to the technical field of natural product extraction.The method comprises the steps that a fresh eucommia ulmoides raw material is subjected to low-temperature drying and smashing, a polysaccharide water extract and filter residues are obtained through water extraction, and then the flavone residual quantity in the polysaccharide water extract is synchronously detected; carrying out enzymolysis on the polysaccharide water extract through beta-glucanase to obtain a small molecular polysaccharide solution, and carrying out alcohol extraction on filter residues to obtain a flavone alcohol extract; the preparation method comprises the following steps: mixing the two components according to a ratio, inoculating compound probiotics for anaerobic fermentation, regulating according to the residual quantity of flavone, regulating an alcohol extraction process by using short-chain fatty acid generated by fermentation when the residual quantity is high, supplementing flavone extract in an enzymolysis system when the residual quantity is low, and finally removing impurities, purifying, adapting intestinal tracts and drying to obtain the product. According to the method, regulation and control operation is correspondingly executed by detecting the residual quantity of flavone in the polysaccharide water extract, so that self-supply of a regulation and control reagent and high efficiency of active ingredients are realized, the conversion rate of small molecular polysaccharide and flavone aglycone is increased, and the intestinal tract tolerance of the product is optimized.

The invention discloses a method for inducing plant disease resistance by using a hydrolase active mutant of xyloglucan specific glucanase XEG1. A pichia pastoris eukaryotic expression system is utilized to obtain the purified hydrolase activity mutant XEG1E136D protein of the phytophthora sojae core pathogenic factor xyloglucan specific glucanase XEG1, and the hydrolase activity mutant XEG1E136D protein does not have the hydrolase activity of plant cell walls and can activate the systemic resistance of plants. The XEG1E136D is overexpressed into a biocontrol bacterium trichoderma harzianum by utilizing an agrobacterium-mediated fungal transformation optimization system, and the XEG1E136D is proved to be capable of secreting XEG1E136D protein to the outside of cells and activating the broad-spectrum systemic resistance of plants. The XEG1E136D protein expressed by the trichoderma harzianum can be used as a plant vaccine to improve the prevention and treatment effect, and the trichoderma harzianum XEG1E136D protein has a wide application prospect in the field of plant disease prevention and treatment.

The invention belongs to the technical field of feed additives, and particularly provides a compound preparation for improving the digestibility of dry coconut shreds, which comprises a compound enzyme preparation, the compound enzyme preparation comprises xylanase with the enzyme activity of 0.5 million U / g to 25 million U / g, glucanase with the enzyme activity of 0.8 million U / g to 200 million U / g, mannase with the enzyme activity of 10,000 U / g to 200 million U / g, cellulase with the enzyme activity of 1.2 million U / g to 25 million U / g, pectinase with the enzyme activity of 10,000 U / g to 25 million U / g, galactosidase with the enzyme activity of 10,000 U / g to 300 million U / g and phytase with the enzyme activity of 0.8 million U / g to 200 million U / g. According to the compound preparation provided by the invention, macromolecular substances such as starch in the dry coconut shreds can be degraded into micromolecular substances which are easy to digest, so that the digestion utilization rate is greatly increased; fat is degraded, fatty acid components such as palmitic acid, oleic acid and lauric acid in the coconut shreds are released, and the effects of reducing blood pressure, promoting digestion and the like are achieved; meanwhile, the generated active substances can improve the disease resistance of animals, improve the health state of organisms, improve the nutritional value of the dried shredded coconut and increase the variety of unconventional feed raw materials.

The invention provides an endoglucanase mutant and application of the endoglucanase mutant. The endoglucanase mutant comprises: 1) a protein which has at least one of the following sites in SEQ ID NO: 29: D194, T232 or G247, has a mutated amino acid sequence, and has endoglucanase activity; or 2) a protein which has an amino acid sequence with homology of at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95% or at least 99% with the protein in 1) and has endoglucanase activity. Compared with a wild type, the endoglucanase mutant has higher specific activity and is beneficial to wide application of beta-endoglucanase in industry.

The invention belongs to the technical field of gene engineering and enzyme engineering, and particularly relates to a glucanase mutant and construction and application of a gene sequence of the glucanase mutant, and the glucanase mutant has an amino acid sequence as shown in SEQ ID NO.2. The glucanase mutant is obtained by carrying out G214A mutation, S249P mutation and D286L mutation on an amino acid sequence as shown in SEQ ID NO. 1. The glucanase mutant has the advantages that the glucanase mutant is obtained; compared with the non-mutated EGL, the glucanase mutant EGL62 provided by the invention has the advantages that the thermal stability is obviously improved; after incubation at 75 DEG C for 3 minutes, the enzyme activity of 60% or above can still be maintained; the enzyme activity is still more than 50% after the enzyme is treated for 1 hour under the condition that the pH value is 3.0-7.0. The glucanase mutant EGL62 provided by the invention has the optimal pH value of 5.0 and the optimal temperature of 50 DEG C, and is good in stability and wide in application prospect.

The invention discloses an extracellular vesicle and a preparation method and application thereof, and belongs to the technical field of cosmetics, and the preparation method comprises the following steps: mixing rose and a PBS buffer solution, juicing, and filtering to obtain a filtrate; adding cellulase, xylanase and beta-glucanase into the filtrate, carrying out enzymolysis, and filtering to obtain an enzymatic hydrolysate; and centrifuging the enzymatic hydrolysate, filtering, resuspending and precipitating to obtain the rose extracellular vesicles. The rose extracellular vesicles can guarantee structural integrity and biological activity, can effectively load active ingredients, and can effectively deliver active molecules in the rose extracellular vesicles to target cells through interaction with membranes of skin cells. The extracellular vesicles can be fused with skin cell membranes to enter cells, active ingredients encapsulated by the extracellular vesicles are released, and the absorption efficiency of skin is improved.

The invention provides an enzymolysis method of oat milk for promoting proliferation of lactobacillus plantarum and bifidobacterium. According to the invention, through the synergistic effect of in-situ enzymolysis of oat endogenous protease and glucanase and enzymolysis of free enzyme, on the basis, through enzymolysis of proper protease, the obtained oat milk contains oat polypeptide and oat beta-glucan with proper molecular weights, and has remarkable activity of promoting proliferation of lactobacillus plantarum and bifidobacterium.

The invention discloses an efficient extraction method of large-molecular-weight astragalus polysaccharide, which belongs to the technical field of plant extraction, and comprises the following steps: cleaning astragalus, filtering, and crushing; the preparation method comprises the following steps: mixing a beta-1, 3-glucanase aqueous solution and trehalose ethylene diamine, and stirring for 10-30 minutes to obtain an enzyme protection system; soaking the radix astragali powder in a citric acid solution, adding an enzyme protection system, applying a bipolar pulsed electric field, and reacting at 40-60 DEG C for 30-40 minutes; adjusting the concentration of the ethanol to 40-50%, standing, centrifuging and collecting crude astragalus polysaccharide; purifying, decolorizing, concentrating, freeze-drying and crushing the crude astragalus polysaccharide to obtain refined astragalus polysaccharide; the mass ratio of the trehalose ethylene diamine to the beta-1, 3-glucanase is (15: 1) to (30: 1). The enzyme protection system maintains the activity of the enzyme, the enzyme and the pulsed electric field act synergistically, the extraction time is shortened, and the extraction rate is increased while the astragalus polysaccharide with large molecular weight is obtained.

The invention relates to the technical field of microorganisms, and particularly provides application of bacillus amyloliquefaciens BA-89 in crop growth promotion and verticillium dahliae antagonism. The strain is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.36498. The strain can secrete multiple extracellular enzymes (including protease, amylase, cellulase and glucanase), has the growth promoting characteristics of producing ammonia, dissolving potassium and the like, and can tolerate high temperature and ultraviolet stress; the compound has a remarkable antagonistic effect on various plant pathogenic bacteria (such as verticillium dahliae, fusarium oxysporum and phytophthora capsici); by inoculating the strain on cotton, the plant growth can be obviously promoted, the plant height, stem diameter, root length, leaf area and biomass are improved, and meanwhile, the disease index of verticillium wilt and the colonization amount of pathogenic bacteria at the root are effectively reduced. The strain disclosed by the invention can be used as a biological fertilizer and a biological pesticide, is used for cotton cultivation and disease prevention and control, and has a wide application prospect.

The invention discloses a method and a system for producing high-purity medium-chain fatty acid (MCFA) based on waste molasses two-stage regulation fermentation. Comprising the following steps: (1) diluting waste molasses, then adding a compound enzyme agent of pectinase, beta-glucanase and cellulase, and coupling microwave to assist treatment; (2) inoculating the pretreated waste molasses with a composite flora containing a clostridium kluyveri mutant strain and a Megasphaera elsdeni engineering bacterium, and carrying out a fermentation treatment on the waste molasses, wherein the composite flora contains the clostridium kluyveri mutant strain and the Megasphaera elsdeni engineering bacterium; (3) sequentially performing two-stage dynamic regulation and control fermentation on the waste molasses inoculated with the composite flora through a pH-stat control strategy to realize efficient conversion from butyric acid to hexanoic acid / octanoic acid; and (4) a hydrophobic hollow fiber membrane module is adopted to realize on-line removal of the product, and the inhibition effect of the product is relieved. According to the technology, the yield of the MCFA produced from the waste molasses reaches 0.45 g / g COD, the cost is reduced by 68% compared with that of a traditional substrate, and a new way is provided for recycling of sugar manufacturing waste.

The invention discloses a low-temperature-resistant and drought-resistant straw degrading bacterium and application thereof. The low-temperature-resistant and drought-resistant straw degrading bacterium is obtained through screening, is stenotrophomonas maltophilia GF-Y18, has good capacity of producing exon-beta-1, 4-glucanase, laccase and lignin peroxidase, can effectively promote straw decomposition, has certain low-temperature-resistant and drought-resistant capacity, can be used for degrading straw, and can be applied to the field of straw degradation. The strain and the fungicide thereof can be used for degrading lignin and / or cellulose and / or straw in a low-temperature drought environment.

The invention discloses a method for generating rare ginsenoside Rh2, which comprises the following steps: adding common glycol type ginsenoside into aspergillus brazilis fermentation liquor for fermentation, and sterilizing after fermentation to obtain a fermentation product; and carrying out enzymolysis by using beta-glucanase and pectinase, and carrying out enzyme deactivation after the enzymolysis is finished, so as to obtain the rare ginsenoside Rh2. The invention also provides the rare ginsenoside Rh2 prepared by the method and application of the rare ginsenoside Rh2. The common glycol type ginsenoside is efficiently and quickly converted into the rare ginsenoside Rh2 through the synergistic interaction of the aspergillus brazilis, the beta-glucanase and the pectinase; the rare ginsenoside Rh2 has the advantages of being simple in preparation method, easy to implement, low in cost, environmentally friendly, free of pollution and the like, and the generated rare ginsenoside Rh2 shows excellent skin care and repairing effects and has wide market prospects when applied to cosmetics.

The present invention relates to a low-nucleic acid yeast protein and a preparation method thereof, and belongs to the field of microbial application technology. The present invention creatively adds xylitol and sodium glycine to a fresh yeast dispersion prepared with deionized water as a dispersion medium, then performs a denucleic acid treatment under alkaline and high temperature conditions, obtains a heavy phase after subsequent centrifugation, and then continues to use deionized water as a dispersion medium to prepare a heavy phase dispersion, and successively adds two different enzymes, namely glucanase and β-mannanase, for enzymatic hydrolysis, thereby further reducing the nucleic acid content and increasing the protein content, thereby preparing a low-nucleic acid yeast protein, wherein the nucleic acid content can be less than 1.5wt%, and the protein content can be greater than 75wt%, effectively expanding the use field of yeast protein.

The present invention relates to the technical field of bacterial enzyme fermentation, and provides a bacterial enzyme synergistic fermentation method for removing antinutritional factors in soybean meal, the method comprises the following steps: S1, soybean meal pretreatment: soybean meal, water and an auxiliary agent are mixed and then subjected to steam explosion treatment, and the auxiliary agent comprises citric acid, a hydrogen peroxide solution and polyphenol; s2, bacterial enzyme fermentation: mixing the pretreated soybean meal with molasses and water to obtain a raw material, adding a complex microbial inoculant and a complex enzyme, uniformly stirring, and fermenting; the complex microbial inoculants comprise saccharomycetes, bacillus subtilis, lactobacillus rhamnosus and pediococcus pentosaceus; the compound enzyme comprises acid protease, pectinase, phytase, glucanase, alpha-galactosidase and keratinase; and S3, after fermentation is finished, drying the materials to obtain the fermented soybean meal. According to the fermentation method, the glycinin degradation rate can reach 79% or above, the acid soluble protein content is increased to 26% or above, the anti-nutritional factor content is remarkably reduced, and the nutritional value is greatly increased.

The present invention relates to polypeptides having endo-1, 3-alpha-glucanase activity, polynucleotides encoding the polypeptides, nucleic acid constructs, vectors, host cells comprising the polynucleotides, methods of producing the polypeptides, and oral care compositions comprising the polypeptides.

The invention relates to the technical field of microorganisms, in particular to bacillus licheniformis JSF-9 and a complex microbial inoculant and application thereof.The strain JSF-9 is obtained by being separated from healthy cattle rectum contents by a research group, and it is detected that the strain has the good beta-1, 4-glucanase producing capacity, and the beta-1, 4-glucanase producing capacity is high. The antibacterial effect on staphylococcus aureus, salmonella typhimurium and shigella flexneri is achieved; the strain has a good bagasse fermentation capability, for example, after the strain, bacillus altitudinis MLF-1 and bacillus safensis MLL-5 are prepared into a mixed microbial inoculum, the bagasse fermentation capability is better, and verification shows that the complex microbial inoculum can effectively inhibit the reproduction of harmful bacteria, so that the preservation period of fermented feed is prolonged, and the yield of the fermented feed is improved. Animal feeding experiments find that the nutritional value and the disease resistance of the complex microbial inoculant fermented bagasse are remarkably improved, 10-15% of the complex microbial inoculant fermented bagasse is added into basic ration, and the cost-reducing and effect-increasing effects are achieved for feeding Jersey cattle.

The invention relates to the technical field of agriculture, and discloses a synergistic technology for repairing soil and promoting plant stem cells by using modified amino active groups. Comprising the following steps: modifying an amino acid compound, namely modifying active groups in amino acid and fulvic acid, so that the active groups have a synergistic effect with cellulase, hemicellulase, urease, phosphatase, chitinase, beta-glucanase, superoxide dismutase, peroxidase and catalase; through the strong chelation of the modified amino active groups and heavy metal ions in soil, the bio-availability and mobility of heavy metals can be effectively reduced, for example, the chelation removal rate of cadmium, lead and other heavy metals can reach 40% or above, the heavy metal contaminated soil is repaired, the soil structure is improved, the content of organic matters in the soil is increased, and the pH value of the soil is adjusted; the acidified or alkalized soil is gradually restored to a pH range suitable for plant growth, and the water and fertilizer retention capability of the soil is improved.

The invention discloses an incision beta-1, 3-glucanase mutant with improved enzyme activity and thermal stability, and belongs to the technical field of gene engineering and enzyme engineering. According to the invention, a site-directed mutagenesis technology is utilized to carry out molecular modification on wild type beta-1, 3-glucanase, and a series of mutants S22F, N226D and N187S are obtained. Compared with a wild enzyme, the beta-1, 3-glucanase mutant constructed by the invention has the advantages that the enzyme activity is improved by 1.04-1.81 times, the Tm value is improved by 0.97-1.19 DEG C, and the half-life period at 70 DEG C is prolonged to 1.08-1.28 times. According to the invention, the enzyme activity and the thermal stability of the wild beta-1, 3-glucanase are improved at the same time, and better use conditions are created for the enzyme in practical application.

The invention relates to the technical field of cyanobacterial bloom control in water, and particularly discloses a compound enzyme-bacterium synergistic preparation for cyanobacterial bloom control, a preparation method and a use method. The preparation comprises the following components in percentage by mass: compound enzymes (10-20% of cellulase, 8-12% of beta-1, 3-glucanase and 3-8% of lipase), functional bacteria (10-20% of bacillus subtilis, 8-15% of trichoderma harzianum and 5-10% of bacillus amyloliquefaciens), an enzyme stabilizer (3-8% of trehalose), an adsorption carrier (15-25% of Fe < 3 + > modified charcoal and 8-15% of diatomite) and auxiliary components. The preparation method comprises the steps of carrier modification, solid fermentation and microcapsule embedding. During use, 3-20kg / 1000m < 3 > of water is added for the first time, 2-10kg / 1000m < 3 > of water is added every 7-14 days in the maintenance stage, and synchronous aeration is performed. Through the synergistic effect of beta-1, 3-glucanase and trichoderma harzianum, the algicidal rate is 92%, and the spore germination inhibition rate is 82%; the Fe < 3 + > modified charcoal improves the stability of the enzyme in an alkaline environment (80% of activity is retained when the pH value is 9); the method is suitable for eutrophic water with chlorophyll a greater than or equal to 30 [mu] g / L, and has no chemical residue risk.