37 results about "Glucanase" patented technology

The present application provides a kind of endo beta-1, 3-glucanase and its application, the amino acid sequence of the endo beta-1, 3-glucanase is SEQ ID NO:1.The endo beta-1, 3-glucanase of the present application can efficiently hydrolyze laminarin and curdlan, its enzyme activity can still be kept above 80% in the range of 40-60 DEG C and pH 4.5-6.0, and it shows good stability under low temperature conditions, which is conducive to reduce the energy consumption in the process of practical application.At the same time, the enzyme has significant high salt tolerance, and has certain resistance to 5%-15% ethanol.The endo beta-1, 3-glucanase of the present application has obvious inhibitory effect on the formation of Candida albicans biofilm, and the laminarin oligosaccharide generated by hydrolyzing laminarin also shows good antioxidant capacity, indicating that the enzyme has broad application prospect and significant practical value in the fields of antibacterial, biological medicine and the like.

The application discloses a high-yield beta-lyase Pichia kluyveri strain breeding and application in beer fermentation, belonging to the field of bioengineering technology. Pichia kluyveri The Pichia kluyveri (Pichia kluyveri) B21 is preserved in the China General Microbiological Culture Collection Center, and the preservation number is CGMCC No. 34028. The strain can produce high-yield beta-lyase, the enzyme catalyzes the production of volatile mercaptans, and can impart beer fruity aroma. The Pichia kluyveri B21 produces high content of ethyl acetate, isoamyl acetate, isobutyl acetate, ethyl nonanoate, ethyl decanoate, phenethyl acetate, linalool and nerol in hop simulation culture medium, and these volatile substances can increase beer fruity aroma, floral aroma and rich beer flavor. The Pichia kluyveri B21 is expected to be added to the production of other food and beverage to enrich flavor substance components.

A food or beverage ingredient is produced from enzymatically hydrolysed cereal, by contacting a slurry of cereal bran with an enzyme composition including xylanase, alpha-amylase, beta-glucanase, cellulase and endoprotease. A beverage creamer composition may contain the food or beverage ingredient.

The present application belongs to the technical field of packaging materials. More particularly, it relates to a PVA adhesive tape adhesive and a preparation method thereof. The specific preparation steps of the present application include: after guar gum and water are mixed and swelled according to a mass ratio of 1:15-20, 0.3-0.5% of endo-beta-glucanase of the mass of the guar gum is added, under the conditions of pH 5.0-5.2, temperature 48-52 DEG C, ultrasonic frequency 50-60 kHz, ultrasonic enzymolysis reaction for 30-45 min, enzyme inactivation by heating, cooling, and then activated plant gum solution is obtained; after the PVA solution and the activated plant gum solution are stirred and mixed uniformly, a thickening agent solution is added, and after continued stirring and uniform mixing, a composite modifier and potassium persulfate are added, under the condition of temperature 40-45 DEG C, incubation and maturation reaction for 2-4 h, aging for 24-36 h, and then discharging, a mixed glue solution is obtained; after the mixed glue solution is coated and dried, a PVA adhesive tape adhesive is obtained.

Recombinant yeasts bioengineered to overexpress genes for utilization of cellulosic and hemicellulosic fibers and / or produce lipid, and methods of use thereof. The yeasts are modified to express, constitutively express, or overexpress an exo-cellobiohydrolase I, an exo-cellobiohydrolase II, an endoglucanase, an endoxylanase, or any combination thereof. The yeasts may also be modified to express, constitutively express, or overexpress a lytic polysaccharide monooxygenase, an arabionofuranosidase, a xylanases, an acetylxylan esterase, or any combination thereof. The methods include cultivating the recombinant yeasts to convert low value organic stillage byproducts such as cellulose and hemicellulose into lipids suitable for biodiesel production and other higher value uses.

This invention discloses an endoglucanase mutant, TrepCel4-Aa, and its applications. The amino acid sequence of the TrepCel4-Aa mutant is shown in SEQ ID No. 4. It is obtained by mutating histidine at position 201 to tryptophan and glutamic acid at position 252 to tyrosine from the endoglucanase with the amino acid sequence SEQ ID No. 1. The nucleotide sequence of the gene encoding this mutant is shown in SEQ ID No. 5. The TrepCel4-Aa mutant contains only one module region of glycoside hydrolase family 5 and has been successfully expressed in *E. coli*. The purified enzyme solution exhibits good activity and excellent hydrolytic ability against natural lignocellulose. This not only enriches the strain sources of endoglucanase but also has theoretical guiding significance for improving the digestibility of feed fiber and enhancing animal production performance.

This invention provides a thermosensitive peppermint essential oil microcapsule and its preparation method. First, peppermint essential oil is encapsulated in yeast cells to prepare microcapsules, improving the problems of volatility and poor stability of peppermint essential oil. Second, the microcapsules are immersed in Bacillus subtilis fermentation broth, where enzymes such as glucanase and mannoglucase in the fermentation broth enzymatically modify the microcapsules, partially converting polysaccharides on the cell wall into oligosaccharides and hydrolyzing long molecular chains into shorter ones, thus improving the dispersibility of the microcapsules in water. Finally, the enzymatically modified microcapsules are immersed in a polylysine-carboxymethyl chitosan solution for surface modification, ultimately obtaining thermosensitive peppermint essential oil microcapsules, broadening the application of peppermint essential oil in the field of food antibacterial preservation.

This invention discloses a strain of *Bacillus belyssus* S2 and its application in the control of early blight in tomato, belonging to the field of microbial technology. *Bacillus belyssus* S2 is deposited at the China Center for Type Culture Collection (CCTCC) on March 30, 2026, with accession number CCTCC NO: M 2026551. The *Bacillus belyssus* S2 isolated and screened in this invention can secrete siderophores, cellulases, proteases, 1,2-β-glucanases, and lipases. Its soluble and volatile substances can inhibit the mycelial growth of tomato pathogens and cause mycelial malformation, reduce spore germination rate, delay the onset of early blight in tomato fruits, and enhance the fruit's resistance, providing a new microbial resource for the control of diseases such as early blight in tomato.

The application belongs to the field of bioengineering, and particularly relates to a beta-1,6-glucanase and application thereof, more particularly to a beta-1,6-glucanase, a composition containing the same, and a method for preparing soluble yeast glucan by using the enzyme or the composition. The amino acid sequence of the beta-1,6-glucanase provided by the application comprises the sequence shown in SEQ ID NO. 1. The beta-1,6-glucanase provided by the application has a wide working temperature range and pH range, good thermal stability and pH stability, and a wide use range, and solves the problems of complex environment, poor pH and temperature tolerance in the industrial application of the beta-1,6-glucanase. Meanwhile, the combination of the beta-1,6-glucanase and beta-1,3-glucanase provided by the application is used to efficiently produce soluble yeast glucan, the reaction condition is mild, and an effective solution is provided for the degradation of natural complex beta-1,3 / 1,6-glucan.

The application discloses a method for preparing high-value cellobiose from a medicine and food homologous Radix Millettiae and application of the high-value cellobiose. The method comprises the following steps: extracting cellulose from Radix Millettiae; optimizing a beta-glucanase enzymolysis process by using a response surface method; after the enzymolysis liquid is subjected to selective removal of glucose through yeast fermentation, the high-value cellobiose product is obtained through acetone precipitation and freeze-drying. The process condition is mild and environment-friendly, high-value utilization of agricultural waste is realized, the obtained cellobiose product has high purity, and an economic and effective approach is provided for large-scale production of cellobiose. Animal experiments prove that the cellobiose product has a significant relieving effect on colonitis induced by dextran sulfate sodium in mice, can effectively improve disease symptoms, reduce oxidative stress, repair intestinal barrier and regulate inflammatory response, and has a good application prospect in preparation of functional food or medicine for relieving colonitis and high-value utilization of Radix Millettiae.

This invention provides a method for mutagenesis of *Aspergillus cristatus* and its application, belonging to the field of microbial technology. The mutagenesis method provided by this invention includes the following steps: first, pretreating *Aspergillus cristatus* suspension by mixing it with a compound enzyme solution; then culturing the pretreated *Aspergillus cristatus* in a culture medium supplemented with a mutagen; the compound enzyme solution consists of a cell-lysing enzyme solution and a β-glucanase solution; the mutagen includes ethyl methanesulfonate and / or diethyl sulfate. This invention can improve the efficiency of the mutagen entering the bacterial cells, thereby enhancing the mutagenesis effect; the mutant strains obtained by mutagenesis, compared to the original strain, exhibit more vigorous colony growth on plates, larger average diameter, denser hyphae, and / or better morphological stability. Furthermore, it can achieve ideal mutagenic selection pressure at lower mutagen concentrations and shorter treatment times, reducing sublethal damage, which is beneficial for the recovery and screening of mutant strains and improving the efficiency of mutagenesis experiments.

This application relates to the field of biotechnology and discloses a protein construct, a polynucleotide, and a recombinant host cell comprising a polypeptide having 1,3-α-glucanase activity. The polypeptide can be used in oral care products, including a polypeptide with the sequence shown in SEQ ID NO:3; and at least one oral care ingredient; wherein the polypeptide has activity in inhibiting and / or clearing biofilm formation of *Streptococcus mutans*. This application provides a polypeptide that inhibits the formation of *Streptococcus mutans* biofilm in the oral cavity, and an enzyme variant with biofilm-clearing effects, the enzyme variant providing stronger biofilm-clearing ability and exhibiting enzyme activity at oral pH levels.

This invention discloses a method for preparing Ganoderma lucidum and jujube seed compound freeze-dried flash release tablets by enzymatic hydrolysis and fermentation, which relates to the field of traditional Chinese medicine preparation technology. The method includes the following steps: (1) Raw material pretreatment: Jujube seed is crushed and mixed with citrate buffer to obtain jujube seed slurry; Ganoderma lucidum fruiting body is ultra-finely pulverized and mixed with cut lily petals to obtain mixed raw materials. This invention uses a specific ratio of compound enzymes (cellulase, pectinase, β-glucanase) in an optimized acidic environment for enzymatic hydrolysis, which can efficiently and selectively destroy the plant cell walls of jujube seed, Ganoderma lucidum and lily, and fully release the active ingredients in the cells; the subsequent fermentation of Lactobacillus plantarum can further decompose the substrate and may produce new beneficial metabolites, forming a synergistic biotransformation, which greatly improves the extraction rate of total active ingredients and product value.

This disclosure relates to a method for preparing an upgraded reconstituted material from dried raw materials with a moisture content of 0-30% w / w, said raw materials comprising lignocellulose and generally derived from waste grains and / or pseudo-grains, and / or food waste, or lignocellulose biomass or mixtures thereof, the method comprising: a) a pretreatment comprising the steps of: i) grinding and / or pulverizing the raw materials to obtain powder capable of passing through a sieve having a sieve aperture size of 10-1000 μm; ii) performing autohydrolysis; subsequently b) enzymatically hydrolyzing the powder obtained in step ii), wherein said enzymatic hydrolysis comprises adding an enzyme mixture comprising at least glucanase and xylanase; and c) a posttreatment comprising the step of grinding and / or pulverizing the enzymatically hydrolyzed mixture obtained in step b) to obtain an upgraded reconstituted material in a flour-like product form.

The application discloses high-digestibility yeast protein and a preparation method and application thereof. First, ordinary yeast protein powder is prepared into a yeast protein dispersion, then the protein structure and aggregation state are regulated by two-stage ultrasonic treatment under temperature control, and then glutaminase and beta-glucanase are sequentially added for enzymatic modification, thereby obtaining high-digestibility yeast protein. By the modification strategy of two-stage ultrasonic pretreatment and sequential double-enzyme treatment, the comprehensive regulation of the aggregation state, molecular conformation and polysaccharide barrier effect of the yeast protein is realized, and the overall method has good technical effect and application value. The method combines physical modification and enzymatic modification, disperses, depolymerizes and restructures the protein by two-stage ultrasonic treatment, and then adjusts the protein conformation and polysaccharide barrier by glutaminase and beta-glucanase respectively.

The application discloses endoglucanases derived from the intestinal flora of Phyllobius oblongus and application thereof. Eight high-activity endoglucanases are obtained from the intestinal flora of Phyllobius oblongus by metagenomics technology, the amino acid sequences of the endoglucanases are respectively shown as SEQ ID NO:1, 3, 5, 7, 9, 11, 13 and 15, and the nucleotide sequences of the encoding genes are respectively shown as SEQ ID NO:2, 4, 6, 8, 10, 12, 14 and 16. The above-mentioned genes are heterologously expressed in Zymomonas mobilis, purified and subjected to enzymatic property determination, and it is confirmed that the endoglucanases have excellent enzymatic activity, and the specific enzyme activity of CSP2705 is as high as 22.1 U / mg. The endoglucanases can be efficiently expressed in Zymomonas mobilis, and provide key enzyme elements for constructing CBP strains with Zymomonas mobilis as a chassis and realizing direct conversion of lignocellulose into fuel ethanol, and have important industrial application value.

The application belongs to the field of food, and particularly relates to a yeast enzymatic hydrolysate with high emulsification performance, a yeast protein, a yeast protein sodium salt product, and preparation and application thereof. The emulsification activity of the yeast enzymatic hydrolysate is greater than or equal to 25.0 m2 / g, and the emulsification stability is greater than or equal to 77.0%; the emulsification activity of the yeast protein is greater than or equal to 70.0 m2 / g, and the emulsification stability is greater than or equal to 175.0%; the emulsification activity of the yeast protein is greater than or equal to 70.0 m2 / g, and the emulsification stability is greater than or equal to 175.0%. In the application, the yeast is subjected to complex enzymolysis by alkaline protease, flavour protease, glucanase, mannanase and glutamine transaminase, and then subjected to ionization reaction with sodium hydroxide to obtain a yeast protein sodium salt product. The yeast protein sodium salt product has high emulsification activity, good color and flavor, and can be used as a natural emulsifier, thereby solving the problems of limited production of sodium caseinate, unstable supply and high price.

The present invention relates to a process for obtaining a yeast protein comprising the following steps: a) providing a yeast cream; b) exposing the yeast cream to heat plasmolysis at a temperature of 70 to 95°C for 30 seconds to 4 hours, preferably 1 minute to 3 hours, more preferably 40 minutes to 2 hours; b') separating the insoluble fraction and the soluble fraction; c) subjecting the insoluble fraction to the activity of at least one ribonuclease and one glucanase, sequentially or simultaneously, at a temperature of 40 to 65°C, preferably 60°C, for 8 to 24 hours, preferably 18 hours; d) separating the insoluble fraction from the soluble fraction; wherein the insoluble fraction collected in step d) has no taste, a nucleotide content of less than 3% and a true protein content of at least 72%. Step b') is optional. In this case, the whole composition obtained after heat plasmolysis of the yeast cream is subjected to the enzymatic activity.

This invention discloses a recombinant β-glucanase bacterium, its construction, and its application in the preparation of water-soluble β-glucan. Belonging to the fields of genetic engineering and enzyme engineering, the recombinant plasmid pWB980-g9050 is introduced and expressed in the β-glucanase recombinant bacterium. The plasmid contains the gene sequence shown in SEQ ID NO.1 from *Chaetoceros* JY25 with accession number CGMCC No. 6882. The full gene sequence of the recombinant plasmid is shown in SEQ ID NO.2. This recombinant bacterium can be used in the preparation of water-soluble β-glucan. This invention achieves efficient and targeted biotransformation of yeast β-glucan by constructing a recombinant *Bacillus subtilis* strain that efficiently expresses a specific endoglucanase and employing a simple process based on crude enzyme solution fermentation. This not only stably produces water-soluble products with concentrated molecular weight distribution and well-defined structures, but also utilizes mild and environmentally friendly processes, demonstrating excellent potential for industrial application.

This invention relates to the field of microbial application technology, specifically to a low-yeast-flavor yeast protein, its preparation method, and its applications. A method for preparing a low-yeast-flavor yeast protein includes the following steps: preparing a dispersion of high-protein yeast, adjusting the pH to 4-8, inactivating it, centrifuging to collect the heavy phase, and then adding water to obtain yeast milk; enzymatically hydrolyzing the obtained yeast milk using a complex enzyme and adding ethyl acetate, wherein the complex enzyme includes glucanase, mannanase, and lipase; inactivating the obtained enzymatic hydrolysate, centrifuging to collect the heavy phase, and obtaining a low-yeast-flavor yeast protein with a protein content of over 78%, a nucleic acid content of less than 1.5%, and almost no yeast odor or other unpleasant odors, meeting the needs of human nutrition and alternative protein fields.

The application discloses a kind of composite enzyme preparation and preparation method thereof, it is related to enzyme preparation technical field.The composite enzyme preparation of the application at least includes the following weight parts of component: xylanase 10-30 parts, modified cellulase 15-35 parts, glucanase 8-20 parts, protease 5-15 parts, amylase 5-18 parts, pectinase 3-12 parts, phytase 5-25 parts, modified superoxide dismutase composite microsphere 15-40 parts.The composite enzyme preparation provided in the application is added in the daily ration of ruminant, can improve the apparent digestibility of ruminant to nutrient and growth performance, while more safe quality better meat quality can be obtained, improve meat taste, obtain higher economic value.

This invention provides a rumen methane inhibitor and its application, belonging to the field of ruminant farming. The active ingredients of the rumen methane inhibitor of this invention include: *Lactobacillus reuteri* inoculum, *Lactobacillus gasseri* inoculum, *Lactobacillus casei* inoculum, amylase, phytase, xylanase, cellulase, and β-glucanase. The rumen methane inhibitor can effectively regulate the intestinal microbiota of dairy cows, promote the decomposition of fibrous material in the rumen, optimize the rumen fermentation environment, thereby controlling rumen function, reducing methane production, and improving the productivity of ruminants. The rumen methane inhibitor is safe and environmentally friendly, which is conducive to its application in ruminant farming, agriculture, and other fields, and has good practicality.

The application provides a multi-enzyme combination and application for accurately characterizing compost fermentation process, and belongs to the technical field of environmental biotechnology and solid waste resource utilization, and the multi-enzyme combination comprises alpha-glucosidase, alpha-galactosidase, endo-1, 3 glucanase, endo-cellulase and endo-chitosanase. The multi-enzyme combination is determined by screening the compost systems of different raw materials such as wheat straw, corn straw, chicken manure, alfalfa, sludge and the like under different C / N ratios. The multi-enzyme combination can accurately characterize four stages of composting, i.e. temperature rising, high temperature, temperature falling and maturity, and the enzyme activity can be determined by various detection methods such as fluorescence colorimetry and visible light colorimetry, so that the real-time monitoring and state evaluation of the composting process can be realized, technical support for the process regulation and quality release of composting is provided, and the application prospect is wide.

The application discloses a kind of lytic polysaccharide monooxygenase SLPMO10 and application thereof, belong to functional enzyme technical field.SLPMO10 amino acid sequence is as shown in SEQ ID NO.1, the nucleotide sequence of its encoding gene is as shown in SEQ ID NO.2.The enzyme belongs to AA10 family, optimum reaction temperature is 60 DEG C, optimum pH is 8.0, by C1 oxidation mode cleavage cellulose, and specifically produce polymeric degree 6 and 8 chitooligosaccharide acid.SLPMO10 and endoglucanase or complex cellulase synergistic effect, can significantly improve the degradation efficiency of cellulose;Especially with complex cellulase synergistic treatment seaweed powder, reducing sugar yield increases 23.5%.The application has important industrial application value in enzyme method treatment cellulose and seaweed biomass conversion.

The application belongs to the field of traditional Chinese medicine deep processing, and particularly relates to a lignum aquilariae resinatum fermentation product and a preparation method thereof. The lignum aquilariae resinatum fermentation product is obtained by enzyme fermentation of lignum aquilariae resinatum; the enzyme is cellulase, beta-glucanase, lignin peroxidase and neutral protease; the cellulase and the beta-glucanase are added into the lignum aquilariae resinatum at the beginning of fermentation; the lignin peroxidase is added into the lignum aquilariae resinatum after 1 day of fermentation, and the neutral protease is added into the lignum aquilariae resinatum after 3 days of fermentation. The lignum aquilariae resinatum fermentation product obtained by three-stage enzyme regulation of natural lignum aquilariae resinatum fermentation has high content of active ingredients and rich aroma, and can be used as traditional Chinese medicinal materials to improve efficacy, and as excellent spices to improve life quality, and has practical popularization and application value.

This invention belongs to the field of veterinary biological agent technology, specifically relating to a bacterial-enzyme complex preparation for the prevention and treatment of transmissible gastroenteritis (TGEV) in pigs, its preparation method, and its application. The bacterial-enzyme complex preparation comprises microcapsule particles; the core material of each microcapsule particle includes a complex of probiotics and a synergistic enzyme system; the complex of probiotics includes *Lactobacillus rhamnosus*, *Bacillus coagulans*, *Pediococcus pentosus*, and *Kluyveromyces marsupialis*. In the bacterial-enzyme complex preparation for the prevention and treatment of TGEV provided by this invention, the complex probiotics and the synergistic enzyme system form a synergistic effect. Lactic acid secreted by *Lactobacillus rhamnosus* can lower the intestinal pH, disrupting the TGEV survival environment; antimicrobial peptides produced by *Bacillus coagulans* can directly cleave the viral capsid protein; lysozyme can degrade viral envelope polysaccharides; and neutral protease, β-glucanase, and xylanase can destroy the viral adsorption sites on the intestinal mucosa. This quadruple action significantly enhances the antiviral effect.

The present application belongs to the technical field of high-value utilization of biomass resources, and particularly relates to a method for preparing a carbon source for a microbial culture medium based on enzymatic hydrolysis of corncob powder, and more particularly to a method for preparing a low-cost and high-efficiency carbon source suitable for the culture of probiotic bacteria by using a self-produced endoglucanase and xylanase complex enzyme system to enzymatically hydrolyze corncob powder, and applying the method to the large-scale culture of bifidobacteria, and the application of the method in the culture of bifidobacteria and the efficient synthesis of postbiotics.