1119 results about "Glucanase" patented technology

A process for the fractionation of valuable fractions from cereal brans (e.g. wheat, barley and oat brans, and rice polish) is described. In particular, this invention describes a two step process, in which the said bran is first subjected to a combination of enzymatic treatment and wet milling, followed by sequential centrifugation and ultrafiltration, which aims at physically separating the main bran factions, i.e. insoluble phase (pericarp and aleurone layer), germ-rich fraction, residual endosperm fraction and soluble sugars. A second step consists of fractionating cereal brans substantially free of soluble compounds, hence insoluble phase from the above-mentioned first step, by enzymatic treatment with xylanases and / or beta-glucanase and wet milling, followed by sequential centrifugation and ultrafiltration, which aims at physically separating the main fractions, i.e. insoluble phase (remaining cell wall components), protein-rich fraction, soluble hemicellulose and oligosaccharide, and therefore maximizes the extraction rate of valuable cell wall components and aleurone cells from previously cleaned bran.

It is an object of the present invention to provide enzymes that have high endoglucanase activity and yet exhibit high activity even under alkaline conditions, and genes encoding the same. The enzyme according to the invention has the following properties: a) exhibiting endoglucanase activity; and b) capable of completely removing fuzz from regenerated cellulose fabrics at a concentration of 1 mg of the protein / L or below. The enzyme of the invention having endoglucanase activity is a protein comprising the amino acid sequence as shown in SEQ ID NO: 1, 3, 5, 7, 9 or 11; a modified protein thereof exhibiting endoglucanase activity; or a homologue of the protein or the modified protein.

The invention discloses a complex enzyme preparation for feeding piglets. The complex enzyme preparation for feeding piglets comprises the following seven enzymes: acid protease, amylase, xylanase, beta-glucanase, cellulase, pectinase and phytase; and the enzymatic activity ratio of the seven enzymes is 1: 1: (8-10): (3.3-4): 1: (0.5-0.55): (0-0.02). By applying the complex enzyme preparation disclosed by the invention, various anti-nutritional factors in feed can be degraded, the viscosity of chyme in an intestinal tract can be reduced, and the metabolic energy of the feed can be improved; the protein and starch digestibility of the piglets can be improved; excessive reproduction of harmful microorganisms in the intestinal tract can be reduced, damage to the intestinal wall can be reduced, and the microecological balance of the intestinal tract can be regulated; the survival rate and disease resistance of the piglets can be improved, the diarrhea rate can be reduced, and the overall uniformity is improved; and the weight gaining of the piglets can be promoted, and the cultivation cost is reduced.

The invention belongs to the technical field of feed additives, and in particular relates to complex enzyme for a feed. The complex enzyme comprises 7,500 to 15,000U / g of xylanase, 1,000 to 3,000U / g of mannose, 50 to 80U / g of galactosidase, 200 to 700U / g of cellulose, 500 to 1,500U / g of beta-glucanase, 150 to 1,000U / g of alpha-amylase, 2,000 to 10,000U / g of protease and 100 to 200U / g of pectinase. By adding corresponding enzyme preparations, the anti-nutrition function of the complex enzyme is reduced, the feed utilization is improved, and the animal production efficiency is improved, so the problems that the utilization rate of animal feed raw materials is low and the animal production performance is inhibited are effectively solved; meanwhile, the complex enzyme has the advantages of reasonable raw material mixing, good effect, and low overall cost.

The invention discloses a laying hen composite premix, and belongs to the technical field of feed composition. A composite enzyme preparation containing beta-mannase, beta-glucanase, acidic proteinase, phytase, pectinase, and amylase is adopted to eliminate the anti-nutrient factors in soybean meal. Yeast cell wall type mycotoxin absorbent is used to effectively eliminate the hazardous factors that may affect the intestine health and are caused by using a large amount of corns. A stomach fortifying acid is used to effectively adjust the pH value of intestines. The used wood chips in the premix are soft and high in fiber content, do not hurt the intestines, do not pollute the environment, can effectively strengthen the digesting and absorbing abilities of laying hens, and have a stronger bearing and water-absorbing performance. The materials mentioned above are systematically and scientifically used to guarantee that the intestines of the laying hens be in the best state. The premix can improve the immunity and disease resistant performance of laying hens, and makes the laying hens exert the maximal laying ability. Moreover, the water content of manure is reduced, and the economic profit of farms that use the premix is prominently improved.

The invention relates to a compound enzyme preparation for fermentation of pu'er tea, which is formed by compounding 10% to 20% of cellulase, 10% to 20% of pectinase, 5% to 15% of polyphenol oxidase, 5% to 10% of glucose oxidase, 5% to 10% glucoamylase, 5% to 10% xylanase, 5% to 10% of beta-glucanase, 5% to 10% of beta-mannase, 5% to 10% of tannase, 5% to 10% of acidic protease, 5% to 10% of lipase, 1% to 5% of alpha-amylase and 1% to 5% of phytase. By the aid of coordination of the enzyme system, polysaccharide and other substances in tea including cellulose and hemicellulose can be effectively decomposed, so that various physiochemical substances in cells can be dissolved. Meanwhile, oxidation and condensation of tea polyphenol, decomposition of protein, amino acid and carbonhydrate, a series of reactions of various products including polymerization and condensation and the like are accelerated and promoted.

This invention provides novel enzyme compositions using newly identified and isolated C. lucknowense enzymes, including CBH Ib CBH IIb, EG II, EG VI, β-glucosidase, and xylanase II in conjunction with previously identified enzymes CBH Ia, CBH IIa (previously described as Endo 43), and EG V. These enzyme compositions demonstrate an extremely high ability to convert lignocellulosic biomass (e.g., Avicel, cotton, Douglas fir wood pretreated by organosolv) to glucose. CBH Ia and IIb, which both have a cellulose-binding module (CBM) displayed a pronounced synergism with three major endoglucanases (EG II, EG V, EG VI) from the same fungus in hydrolysis of cotton as well as a strong synergy with each other. The enzyme compositions are effective in hydrolysis of the lignocellulosic biomass.

There is provided an endoglucanase enzyme, which is useful for reducing fuzz of regenerated cellulose-containing fabrics, improving the touch and appearance, color clarification, localized variation in color, reducing stiffness and using it as components of a detergent, as well as deinking waste paper and improving freeness of paper pulp. A cDNA coding for the endoglucanase enzyme NCE5 was cloned and its DNA sequence and amino acid sequence derived from it were determined.

The invention provides a biocontrol bacterial strain for preventing and treating plant diseases and its bacterial agent, belonging to the field of biological control. The strains used are Gram-negative bacteria, identified as Lysobacter enzymogenes, and the strain code is OH11. Strain OH11 has no flagella but has slippage, can produce various extracellular hydrolytic enzymes including chitinase, β-1,3-glucanase and protease, and can effectively inhibit the growth of fungi and bacteria. The antibacterial zone diameters of strain OH11 against Sclerotinia sclerotiorum and Phytophthora capsici were both greater than 22.0 mm; the antagonism against potato ring rot was stronger, and the diameter of the inhibition zone reached 50 mm. The OH11 strain was inoculated into the seed tank, cultivated to the logarithmic growth phase, and the seed liquid was connected to the production tank for cultivation. The medium used in the production tank was the same as that of the seed tank. The liquid fermentation adopts aerobic submerged fermentation and fed-batch process, the dissolved oxygen is 15%-20%, the fermentation temperature is 30°C, the fermentation time is 72h, and the initial pH value is 7.5. After the fermentation is completed, the culture solution is taken out of the tank and directly packed into liquid dosage forms with plastic packaging barrels or packaging bottles, or subpackaged into solid dosage forms with peat adsorption packaging bags. The biocontrol strain OH11 can effectively control plant pathogenic fungi, bacteria, nematodes and other diseases, and the overall control effect is 50%-70%. In the greenhouse pot experiment, the control effects of OH11 on pepper blight and tomato bacterial wilt reached 83.6% and 86.4%, respectively. Strain OH11 has the characteristics of broad antibacterial spectrum, high activity, and environmental safety. In today's serious pesticide pollution, zymolysobacterium and its bacterial agent will be a good substitute.

The invention relates to an animal feed additive containing compound enzyme. The additive is a microecological compound enzyme preparation mainly composed of xylanase, mannanase, beta-glucanase, cellulase, acid protease, alpha-amylase, phytase, Bacillus subtilis, Bacillus licheniformis and the like. The product of the additive containing compound enzyme in the invention can be used as a novel feed additive, has no drug residue, no pollution and no generation of drug resistance, drug residues and drug-resistant strains, can be extensively applied to livestock feeds and ruminant feeds and has effects on improving the digestion and utilization rate of feeds, enhancing production performance of animals, strengthening immunity of animals, etc.

A novel endoglucanase derived from Staphylotrichum coccosporum, a polynucleotide encoding the endoglucanase, and a cellulase preparation containing the endoglucanase are disclosed. The endoglucanase or cellulase preparation is available for a washing use or fabric processing, such as a color clarification of a cellulose-containing fabric, a reduction of fuzz, an improvement of the touch feel and appearance of the fabric, providing a localized color change to the fabric, or a reduction of stiffness.

This inventnion relates to a preparation method for high-efficiency prebiotics oat beta-glucan, belonging to the quintessential processing technologies of oat. The said method includes using oat bran as raw materials, crushing, microwave-assisted-extracting, adding amylase and glucoamylase, isoelectric-point-precipitating, centrifugating and separating, concentrating the supernatant, precipitating with ethanol, centrifugating and collecting the precipitation, solving with water, hydrolyzing with beta-glucanase, and spray-drying process to obtain the high-efficiency prebiotics oat beta-glucan with significantly enhanced intestinal and fecal bifidobacteria and actobacillus value-added effect. The method is scientifically and rationally designed, and of great significance in achieving the comprehensive utilization of resources oat and increasing its added value.

The invention relates to enzymes having xylanase, mannanase and / or glucanase activity, e.g., catalyzing hydrolysis of internal β-1,4-xylosidic linkages or endo-β-1,4-glucanase linkages; and / or degrading a linear polysaccharide beta-1,4-xylan into xylose. Thus, the invention provides methods and processes for breaking down hemicellulose, which is a major component of the cell wall of plants, including methods and processes for hydrolyzing hemicelluloses in any plant or wood or wood product, wood waste, paper pulp, paper product or paper waste or byproduct. In addition, methods of designing new xylanases, mannanases and / or glucanases and methods of use thereof are also provided. The xylanases, mannanases and / or glucanases have increased activity and stability at increased pH and temperature.

The invention relates to bio-enzyme gel breaker, in particular to formula and technique for gel breaking of water-based guargum fracturing fluid by using the bio-enzyme gel breaker. The bio-enzyme gel breaker contains the following components in percentage by weight: 10-50% of beta- mannose, 0-20% of cellulose, 0-10% of pectinase, 0-15% of glucanase, 0-10% of xanthase, 3-10% of (NH4)2SO4, 2-5% of NaCl, 1.5-5% of ZnCL2 and 0-60% of persulfate. The fracturing fluid gel breaking technique comprises the following steps: dissolving the bio-enzyme gel breaker in borax crosslinked fluid; mixing and blending the crosslinked fluid and the guargum base fluid, crosslinking, forming jelly, and then mixing with proppant, pressing the mixture into the oil-water well until the mixture enters the fractured crack. In this way, the fracturing process is finished. The bio-enzyme gel breaker degrades the macromolecular guargum in the stratum fracturing fluid into small micromolecular sugar, and when the jelly breaks up, the proppant is left underground, the fracturing gel breaking fluid flows back, and in this way, the construction can be completed. The bio-enzyme gel breaker has good gel breaking performance, the construction process is reasonable, the viscosity of the fracturing fluid can not decrease too early, the gel of the fracturing fluid can be broken evenly and thoroughly with little residue and small harm to the stratum, and the operation is simple and convenient.

The invention discloses a high efficiency bioactivity animal feed additive product and a production method and application thereof. The invention pertains to the field of feed additive, which more particularly relates to a high efficiency bioactivity animal feed additive product that comprises various biogenk and a production method and application thereof. The invention solves the technical problem of providing a high efficiency bioactivity animal feed additive product, and the invention solves another technical problem of providing the production method and application of the high efficiency bioactivity animal feed additive product. The ingredients of the product of the invention comprise active bacillus subtilis, active yeast, active plant lactobacillus bacteria, Beta-glucanase, xylanase, cellulose and amylase; the production method of the invention comprises cultivation preparation and compound inocula of microorganism inocula. The product of the invention can improve microecology balance of enteric canal of ruminants, enhance utilization ratio of the feed, improve milkability of milk cows and quality of the milk, improve body immunity of the milk cows, thus reducing enteric canal diseases and purifying raising environments.

The present invention relates to a heterologous exo-endo cellulase fusion construct, which encodes a fusion protein having cellulolytic activity comprising a catalytic domain derived from a fungal exo-cellobiohydrolase and a catalytic domain derived from an endoglucanase. The invention also relates to vectors and fungal host cells comprising the heterologous exo-endo cellulase fusion construct as well as methods for producing a cellulase fusion protein and enzymatic cellulase compositions.

The invention relates to polypeptides having glucanase, e.g., endoglucanase, mannanase, xylanase activity or a combination of these activities, and polynucleotides encoding them. In one aspect, the glucanase activity is an endoglucanase activity (e.g., endo-1,4-beta-D-glucan 4-glucano hydrolase activity) and comprises hydrolysis of 1,4-beta-D-glycosidic linkages in cellulose, cellulose derivatives (e.g., carboxy methyl cellulose and hydroxy ethyl cellulose) lichenin, beta-1,4 bonds in mixed beta-1,3 glucans, such as cereal beta-D-glucans or xyloglucans and other plant material containing cellulosic parts. In addition, methods of designing new enzymes and methods of use thereof are also provided. In alternative aspects, the new glucanases e.g., endoglucanases, mannanases, xylanases have increased activity and stability, including thermotolerance or thermostability, at increased or decreased pHs and temperatures.

The invention discloses a compound enzyme preparation for ramie degumming, and a preparation method thereof. The method comprises the steps of taking bacillus cereus obtained through separation as an original strain, preparing high-enzyme-activity alkaline pectinase which contains glucanase and neutral protease through seed culture and liquid-submerged fermentation, and compounding an appropriate amount of xylanase and mannanase on the basis of the alkaline pectinase so as to prepare the compound enzyme preparation. The invention also discloses a ramie degumming process, and the compound enzyme preparation is applied to an enzyme refining step in the ramie degumming process. The process enables the alkaline pectinase and hemicellulase to play a role jointly by compounding a plurality of biological enzymes, so as to achieve the aim of degumming. The process can save acid-base raw materials, and has the advantages of mild treatment conditions, low cost, good fiber quality, little pollution on environment and the like.

A nucleic acid molecule having a nucleic acid sequence that encodes a linker region of exoglucanase, said nucleic acid sequence comprising the nucleic sequence 5'-GGCGGAAACCCGCCTGGCACCACC-3'.

The invention provides a traditional Chinese medicine multi-enzyme bacterium straw composite feed for livestock and poultry. The feed is mainly prepared from saccharomyces cerevisiae, protease, phytase, cellulose, xylanase, glucanase, laccase, pectase, amylase, Aspergillus niger, traditional Chinese medicinal preparations consisting of Radix Astragali, jerusalem artichoke, Poria cocos, purslane, dandelion and the like, straw, corn flour, potassium dihydrogen phosphate and magnesium sulfate magnesium sulfate. According to the invention, a complex enzyme system and microbes exert action on the straw, and synergism between traditional Chinese medicines and potassium dihydrogen phosphate and magnesium sulfate is used at the same time; so output of bifidobacteria in the livestock and poultry is increased, disease-resistant immunity of the livestock and poultry is improved, and bifidobacteria exerts effects on assisting digestion and decomposing plant barriers in coarse cereals so as to realize easy absorption of the coarse cereals by intestines and stomachs and assist the livestock and poultry in rapid digestion and absorption of foods. Thus, the livestock and poultry like flocks and herds are allowed to have healthy and strong bodies, an enhanced digestive function and an improved farrowing rate, fattening and marketing time is shortened, and breeding cost is reduced.

The present invention relates to xyloglucanases belonging to family 44 of glycosyl hydrolases and having a relative xyloglucanase activity of at least 30% between pH 5 and pH 8 are derived from the genus Paenibacillus, especially from a strain of Paenibacillus polymyxa or Paenibacillus sp. The xyloglucanases exhibit high performance in conventional detergent compositions.

Purified cellobiohydrolase I (glycosyl hydrolase family 7 (Cel7A) enzymes from Penicillium funiculosum demonstrate a high level of specific performance in comparison to other Cel7 family member enzymes when formulated with purified EIcd endoglucanase from A. cellulolyticus and tested on pretreated corn stover. This result is true of the purified native enzyme, as well as recombinantly expressed enzyme, for example, that enzyme expressed in a non-native Aspergilllus host. In a specific example, the specific performance of the formulation using puriified recombinant Cel7A from Penicillium funiculosum expressed in A. awamori is increased by more than 200% when compared to a formulation using purified Cel7A from Trichoderma reesei.

The invention discloses an additive for predigestion fermented feed for piglets, relating to pannage additive. The additive for predigestion fermented feed for piglets can stably improve the digestibility of feed and maintain the microecological balance of intestinal tracts. Each ton of perfect compound feed comprises 800-1000g of complex enzyme preparation, 300-500g of probiotic bacteria and 3000-5000g of lactic acid bacteria liquid, wherein 1g of complex enzyme preparation contains 800-1200U of acid protease, 80-120U of amylase, 1800-2200U of saccharifying enzyme, 12000-18000U of xylanase, 4000-5000U of beta-glucanase, 12000-16000U of cellulose, 6000-9000U of pectinase and 400-600U of phytase; 1g of probiotic bacteria contains 1.0*1010-2.0*1010CFU (colony-forming unit) of Bacillus licheniforms endogenous spore and 1.0*1010-2.0*1010CFU of yeast cell; and 1ml of lactic acid bacteria liquid contains 1.0*109-2.0*109CFU of lactic acid bacteria.