299 results about "Neutral protease" patented technology

The invention discloses preparation of a whey protein peptide mixture with immune and muscle-building effects, and relates to the technical field of food processing and bioengineering. The neutral protease is adopted and the pH is adjusted, so that the enzymolysis efficiency is effectively improved, and the enzymolysis time is greatly shortened; meanwhile, the whey protein peptide which is uniform in molecular weight distribution, free of peculiar smell and good in thermal stability is successfully prepared by accurately controlling enzymolysis conditions and combining microbial fermentation. The protein peptide can effectively improve thymus indexes and spleen indexes, enhance the proliferation capacity of splenic lymphocytes and the activity of NK cells, play an excellent immune regulation role and have a remarkable muscle building effect. The preparation process has the advantages of high efficiency, low cost, simplicity, convenience and the like, gives consideration to high quality and high functionality of the product, and has great application value.

The invention relates to a medicine for repairing skin wounds and a preparation method thereof, and belongs to the technical field of biological medicines.The medicine is prepared from, by mass, 25-30 parts of bee embryo peptide, 8-10 parts of alkannin derivative, 10-15 parts of armillaria mellea polysaccharide, 8-10 parts of inonotus obliquus polysaccharide, 6-8 parts of notoginsenoside and 5-7 parts of astragaloside. The bee fetus peptide is a product which is more than 500Da and is obtained by performing step-by-step enzymolysis on bee larva freeze-dried powder through trypsin and bacillus subtilis neutral protease to obtain enzymatic hydrolysate which is less than 3kDa, performing purification through a G-50 sephadex column, performing ultrafiltration and performing freeze-drying. The alkannin derivative is obtained by reacting alkannin with succinic anhydride and glucosamine-polyethylene glycol. A special method is adopted for preparing the bee embryo peptide and alkannin derivatives, the bee embryo peptide and alkannin derivatives are matched with other components to play a synergistic role, and the effects of high safety, high skin cell proliferation promoting capacity, excellent antibacterial performance and the like are achieved.

This invention relates to the technical field of deep processing of sturgeon by-products, and particularly to a sturgeon tendon peptide with antioxidant and pancreatic lipase inhibitory activities and its preparation method. The preparation method includes the following steps: mixing sturgeon with water and crushing it to form a first slurry; adding Bacillus licheniformis alkaline protease to the first slurry for a first hydrolysis; adding Bacillus subtilis neutral protease to the first hydrolysate for a second hydrolysis; adding a complex enzyme to the second hydrolysate for a third hydrolysis to obtain a sturgeon tendon hydrolysate; filtering the sturgeon tendon hydrolysate to remove impurities to obtain a filtrate, which is then filtered again to retain polypeptides, thus obtaining the sturgeon tendon peptide; wherein the complex enzyme includes pectinase and amylase. Using this method, a sturgeon tendon peptide with high glycosaminoglycan content can be obtained, which exhibits pancreatic lipase inhibitory activity, antioxidant activity, Staphylococcus aureus inhibitory activity, and prebiotic effects that promote the proliferation of Lactobacillus plantarum and Lactobacillus fermentum.

The invention provides a whey protein deep hydrolysate with low sensitization and an allergy relieving effect as well as a preparation method and application of the whey protein deep hydrolysate. According to the method, the alkaline protease, the neutral protease and the glutamyltransferase are sequentially used for carrying out first enzymolysis, second enzymolysis and third enzymolysis on the whey protein raw material, and the whey protein hydrolysate which is low in molecular weight, good in taste, good in digestion and absorption performance, low in sensitization and capable of effectively relieving allergic reaction of the organism is obtained.

The invention discloses a preparation method for simultaneously and efficiently extracting crocodile polypeptide and crocodile oil, and relates to the technical field of biological extraction, crocodile meat is used as a raw material, and heating micro-pressure treatment is sequentially performed to promote component dissolution; a multi-stage enzymolysis process is adopted, alkaline protease, neutral protease and lipase are sequentially used, and a calcium phosphate system is used for assisting filtration and a natural antioxidant is added in a gradient manner in the enzymolysis process; performing plate frame filtration and standing layering subsequently to realize crude separation of the oil peptide; finally, the crocodile polypeptide liquid is subjected to multi-stage membrane filtration and spray drying, the crocodile crude oil is subjected to ultrasonic-assisted fishy smell removal, ceramic membrane fine filtration and gradient cooling phase change refining, and after enzymolysis treatment and before filtration, a composite additive is added into enzymatic hydrolysate and stirred. No organic solvent exists in the whole process, and the product yield, purity and oxidation stability are remarkably improved.

The invention relates to the technical field of active peptides, in particular to a myocardial active peptide capable of relieving heart failure and protecting heart and a preparation method thereof. The method takes pig hearts as raw materials, and sequentially comprises the following steps: pretreating the pig hearts, adding water, and homogenizing; adding alkaline protease to carry out first constant-temperature enzymolysis; adding neutral protease and flavourzyme to carry out second constant-temperature enzymolysis; then lipase is added for third-time constant-temperature enzymolysis, and enzyme deactivation and cooling are carried out; and finally, adding activated carbon into the enzymatic hydrolysate, filtering with diatomite, and spray-drying to obtain the myocardial active peptide. According to the method, the yield and the purity of the active peptide are remarkably improved through a synergistic process of three-step enzymolysis and activated carbon purification, the obtained product is rich in small molecule peptide with a definite heart protection function, and a new way is provided for high-value utilization of pig heart resources and development of functional products for preventing and treating heart failure.

The invention discloses modified hydroxytyrosol as well as a preparation method and application thereof. The provided preparation method of the modified hydroxytyrosol comprises the following steps: in the presence of an acidic solvent, mixing hydroxytyrosol, protease and collagen for reaction, mixing a reaction product with an extraction agent, performing centrifugal layering, and collecting upper-layer components, thereby obtaining the modified hydroxytyrosol, the protease comprises one or two of neutral protease and trypsin. Under the enzymolysis action of protease, the collagen can be decomposed into amino acid monomers such as glycine, proline and hydroxyproline, and the amino acid monomers can be subjected to graft modification with hydroxytyrosol; meanwhile, collagen fragments which are not subjected to enzymolysis also exist, and are subjected to graft modification with hydroxytyrosol in the form of collagen characteristic fragments. The oxidation resistance and the stability of the obtained modified hydroxytyrosol are obviously improved compared with those of unmodified hydroxytyrosol.

The invention discloses a preparation method of micromolecular active collagen peptide, which comprises the following steps: (1) cutting fresh leather into fragments, pretreating the leather with a sodium bicarbonate solution, rinsing the leather with deionized water, and refrigerating and storing; (2) mixing papain, bromelain, pancreatin, pepsase, alkaline protease, neutral protease and flavourzyme to obtain a compound enzyme preparation; (3) performing high-pressure steam sterilization on the leather at 110-140 kPa and 120-122 DEG C to obtain leather with the hydrolysis degree of 75-85%, then adding distilled water into the leather to obtain mucilage, then adding a compound enzyme preparation into the mucilage, reacting for 5.5-6.5 hours at the pH of 6.8-7.2 and the temperature of 50-55 DEG C, and then performing enzyme inactivation at the temperature of 85-95 DEG C; and (4) after the reaction system is cooled to room temperature, purifying and centrifuging the reaction system, and taking clarified supernate, thereby obtaining the product. Different animal skins are treated, the proportion of peptide fragments with the molecular weight smaller than 1000 Da of the prepared small-molecule active collagen peptide is larger than or equal to 80%, and the hydroxyproline content is larger than 3%.

The invention relates to the technical field of collagen extraction, and provides a collagen extraction process for increasing hydroxyproline content, which sequentially comprises the steps of raw material pretreatment, deliming and degreasing, directional enzymolysis, gradient pressure-temperature synergistic extraction, purification, concentration and drying, a composite pretreatment agent containing a proline hydroxylase activator, EDTA-2Na and neutral protease is adopted in the raw material pretreatment; deashing and degreasing are combined with a chelating agent solution and ultrasonic treatment; a compound enzyme solution composed of trypsin, papain and proline hydroxylase is used for directional enzymolysis; a two-stage gradient pressure-temperature cooperation mode is adopted for extraction; purification is realized through combination of gel filtration chromatography and ion exchange chromatography; and finally carrying out freeze concentration and vacuum freeze drying to obtain the product. According to the process, through a multi-step synergistic effect, the content of hydroxyproline in collagen is remarkably increased, the product purity is high, natural functional characteristics are reserved, and the process is suitable for the fields of food, cosmetics, biological medicine and the like.

The invention belongs to the technical field of biological fermentation, and particularly relates to ganoderma lucidum polypeptide and a preparation method and application thereof. The preparation method comprises the following steps: (1) taking ganoderma lucidum powder, adding water, and cooking to obtain a mixture 1; (2) adding bacillus subtilis liquid into the mixture 1, and fermenting to obtain ganoderma lucidum polypeptide fermentation liquid bacteria liquid; (3) adding neutral protease into the ganoderma lucidum polypeptide fermentation liquid for primary enzymolysis, and then adding aminopeptidase for secondary enzymolysis to obtain ganoderma lucidum polypeptide enzymatic hydrolysate; and (4) filtering, concentrating and spray-drying the lucid ganoderma polypeptide enzymatic hydrolysate to obtain the lucid ganoderma polypeptide. The molecular weight of the ganoderma lucidum polypeptide ranges from 200 Da to 5000 Da, molecular weight distribution is uniform, the polypeptide content can reach 31.11% at most, the ganoderma lucidum polypeptide can be applied to functional cosmetics, and the application range of the ganoderma lucidum polypeptide is widened.

The invention discloses extraction of polydeoxyribonucleotide as well as preparation and application of a PDRN-Ce (Polydeoxyribonucleotide-Ce) microsphere cluster. The extraction and purification method comprises the following steps: mixing in-vitro fish testis tissues with water, carrying out crushing treatment and homogenization treatment on an obtained premix, carrying out proteolysis and solid-liquid separation, and collecting supernate to obtain enzymatic hydrolysate; wherein protease used for proteolysis comprises neutral protease; carrying out alcohol precipitation on the enzymatic hydrolysate by adopting an alcohol solvent, and collecting a precipitate; and dissolving the precipitate in water, desalting and drying. According to the extraction method, the purity and the yield of the poly-deoxyribonucleotide are remarkably improved, the preparation method is low in cost and convenient to operate, the prepared poly-deoxyribonucleotide is stable in property, small in molecular weight and easy to absorb by skin, in order to further improve the bioavailability of the extracted poly-deoxyribonucleotide, the PDRN-Ce microsphere cluster is prepared, and the PDRN-Ce microsphere cluster can be used for preparing the poly-deoxyribonucleotide. Good application prospects are realized.

The present invention discloses a Dendrobium officinale protein hydrolysate composition, a preparation method thereof, and an application thereof in anti-aging cosmetics. The Dendrobium officinale protein hydrolysate composition consists of Dendrobium officinale protein hydrolysates TDM-1 and TDM-5, with a mass ratio of 1:(0.5-2); TDM-1 and TDM-5 are peptide components with a molecular weight ≤10 kDa and a peptide component with a molecular weight of 10 kDa < ≤50 kDa, which are obtained by enzymatic hydrolysis of Dendrobium officinale crude protein using a neutral protease and papain. TDM-1 and TDM-5 components have synergistic effects of antioxidant, anti-inflammatory, and barrier repair by inhibiting the activation of the MAPK / NF-κB signaling pathway and promoting ECM repair mediated by the TGF-β pathway, thereby achieving multi-target anti-aging and having low irritation. They can be used in dosage forms such as essences and creams, providing core raw materials for the development of natural anti-aging cosmetics.

The present invention relates to a cartilage tissue single cell extraction kit and an extraction method thereof, the kit comprises: (1) a first enzyme for enzymatic hydrolysis, the first enzyme for enzymatic hydrolysis comprising one or more of trypsin, chondroitinase ABC and fucosidase; (2) a second enzyme for enzymolysis, wherein the second enzyme for enzymolysis comprises collagenase II, neutral protease II, hyaluronidase and Dnase I; and (3) a basic solution, wherein the basic solution comprises a basic culture medium and / or a buffer solution. The invention also provides a method for extracting single cells from cartilage tissues by using the kit. The cartilage tissue single-cell suspension prepared by the method has the advantages of high cell yield, high activity and the like, and has a wide application prospect.

The invention relates to the technical field of microbial fermentation, and particularly discloses a microbial fermentation process for food processing. The composition used in the process comprises lactobacillus plantarum, aspergillus oryzae and a compound enzyme preparation containing neutral protease, cellulase and phytase. The preparation method comprises the following steps: firstly, pretreating the raw materials, then compounding lactobacillus plantarum and aspergillus oryzae into a fermentation starter according to a specific proportion, fermenting in a liquid environment, and implementing dynamic stepped oxygen control; adding a complex enzyme preparation in a hypha growth period; then transferring into solid matrix conversion to construct a porous structure; and finally, carrying out two-stage temperature-controlled vacuum drying to obtain the product. The composition disclosed by the invention can be used for fermenting plant-based raw materials such as beans and grains, and has the advantage that the availability of a substrate can be improved through the synergistic interaction of bacteria and enzymes; in addition, according to the process, through stepped oxygen control, the double-bacterium respiratory rhythm can be precisely adapted, and the problem of metabolism mutual exclusion in a constant oxygen mode is thoroughly solved.

The application provides a preparation method of seafood flavoring agent and shrimp paste, and relates to the technical field of food flavoring agents. The slurry of shrimp offal is subjected to enzymolysis by using a combination of neutral protease, flavor protease, nuclease and lipase, and then subjected to Maillard reaction, so that the seafood flavoring agent with high freshness, low bitterness and good flavor is obtained, and the seafood flavoring agent can be used for preparing shrimp paste.

The invention discloses phospholipid-rich egg yolk polypeptide powder as well as a preparation method and application thereof, and belongs to the technical field of food processing and medical health care. The preparation method of the phospholipid-rich egg yolk polypeptide powder specifically comprises the following steps: beating a fresh egg to separate egg yolk, adding water to dilute, adjusting the pH value to 5.0-6.0, centrifuging, and taking a precipitated phase; adding water to dilute the precipitate, adjusting the pH value to 7.0-9.0, and centrifuging to obtain a slurry phase supernatant; adding neutral protease into the pulp phase supernatant, and carrying out an enzymolysis reaction; after the reaction, concentrating, drying, freezing and crushing; placing the crushed material in a supercritical carbon dioxide extraction device for deoiling, and after deoiling, mechanically crushing and screening to obtain phospholipid-rich egg yolk polypeptide powder; the phospholipid-rich egg yolk polypeptide powder has relatively high dispersibility and stability, also has relatively high activity, has a very good promotion effect in improvement of a cognitive function, shows huge application potential in the technical fields of functional foods, health care products and medicines, and has extremely wide market prospects.

The invention provides a preparation method of a single-cell sequencing suspension fixedly preserved in situ. The invention provides digestive juice for preparing a single-cell suspension. The digestive juice is a solution composed of collagenase II and neutral protease. The invention also provides a kit containing the digestive juice. The invention provides application of the digestive juice to preparation of a kit and application of the kit to in-situ fixed preservation of cells and preparation of a single-cell suspension. The invention also provides a preparation method of the in-situ immobilized preserved cell and the single-cell suspension, and the single-cell suspension prepared by the preparation method.

The invention relates to the field of biological product preparation, in particular to a preparation method of high-activity oyster-derived small molecule peptide. The method comprises the following steps: selecting fresh meat of pacific oysters or ostrea rivularis as a raw material, degreasing, adding water, crushing, filtering, sequentially carrying out compound protease and neutral protease hydrolysis, implanting a sensor and a probe in hydrolysis, monitoring in real time, and dynamically adjusting reaction conditions; adding a targeting microcapsule wrapping an ascorbic acid glutathione composite protective agent, and releasing the protective agent when a target peptide signal is detected; separating and concentrating through a gradient membrane, and purifying the target peptide by using a molecular imprinting chromatographic column; carrying out alkaline hydrolysis on enzymatic hydrolysis residues to prepare chitosan for cyclic utilization, carrying out enzymatic hydrolysis on residual residues to generate collagen peptide, treating and recycling wastewater, and recovering protease. According to the method, the technical effects that the high-activity and high-purity target peptide is prepared, the resource utilization rate is increased, the production cost is reduced, the target peptide has good oxidation resistance and biological activity, the intestinal enzyme degradation rate is reduced after glycosylation modification, and the blood half-life period is prolonged are achieved.

The invention provides high-protein fermented milk as well as a preparation method and application thereof, and relates to the technical field of dairy products. The preparation method of the high-protein fermented milk comprises the following steps: S1, inoculating a fermentation base material with a fermentation inoculant, applying ultrasonic treatment, and finishing the ultrasonic treatment until the ultrasonic energy density of a fermentation system is 10-25kJ / L, the ultrasonic treatment power is 100-200W, and the frequency is 15-25kHz; s2, neutral protease is added into the secondary fermentation system for continuous fermentation treatment, the enzyme activity of the neutral protease is larger than or equal to 5000 U, and the adding amount is 0.01 wt%-0.04 wt%. According to the method, ultrasonic treatment and protease are combined to treat and ferment a high-protein product, so that the fermentation speed is further increased, the system viscosity of the high-protein fermented milk is effectively reduced, the problem of rough texture caused by high protein content is solved, the particle size of the fermented milk is relatively small, and the fineness and stability of the product are improved.

The invention discloses camellia seed meal oligopeptide as well as a preparation method and application thereof. The method comprises the following steps: S1, pretreating camellia seed meal to obtain a pretreated substance; s2, performing enzymolysis and wall breaking to obtain first enzymatic hydrolysate; wherein the enzymolysis wall breaking adopts a mixed enzyme, and the mixed enzyme comprises amylase and cellulase; s3, extracting crude protein to obtain crude protein; s4, carrying out crude protein enzymolysis on the crude protein to obtain a second enzymatic hydrolysate; wherein the crude proteolysis adopts mixed protease, and the mixed protease comprises first protease and second protease; wherein the first protease is one or more of alkaline protease, neutral protease and acid protease; the second protease is papain; and S5, purifying the second enzymatic hydrolysate to obtain the camellia seed meal oligopeptide. The average molecular weight of the obtained product is smaller than 1000 Daltons, and the product has biological activity and high application value in the fields of cosmetics and functional food.

The invention relates to the technical field of microorganism application, in particular to low-nucleic-acid yeast protein as well as a preparation method and application thereof. The preparation method of the low-nucleic-acid yeast protein comprises the following steps: (1) carrying out inactivation treatment on high-protein yeast; (2) carrying out enzymolysis on the inactivated yeast by adopting neutral protease, glucanase, mannase and first nuclease, and after enzyme deactivation, separating and taking a heavy phase to obtain crude yeast protein; (3) performing high-pressure homogenization treatment on the crude yeast protein; and (4) carrying out enzymolysis on the crude yeast protein subjected to high-pressure homogenization treatment by adopting second nuclease and lipase, and after enzyme deactivation, separating and taking a heavy phase to obtain the low-nucleic-acid yeast protein. The yeast protein with the nucleic acid content of 1.0% or below is obtained by taking the high-protein yeast as a raw material through inactivation, compound enzyme enzymolysis, high-pressure homogenization treatment and secondary enzymolysis, is neutral and pure in taste, and can be widely applied to the fields of nutrition, health and food such as protein supplementation and protein substitution.

The invention discloses a hippophae rhamnoides seed meal protein-based hypoglycemic peptide and a preparation method thereof, and the preparation method comprises the following steps: mixing hippophae rhamnoides seed meal powder with water, adjusting the pH value with sodium hydroxide, stirring, and centrifuging to obtain a supernatant; adjusting the pH value of the supernatant with hydrochloric acid, standing, centrifuging, washing the obtained precipitate with distilled water until the precipitate is neutral, pre-freeze-drying, and freeze-drying to obtain SSP powder; the preparation method comprises the following steps: mixing SSP powder with water to obtain an SSP solution, carrying out water bath preheating, adding protease, carrying out enzymolysis, carrying out water bath heating on the solution, adjusting the pH value of the solution to be neutral, collecting a centrifugal supernatant, and carrying out low-temperature dialysis to obtain the hypoglycemic peptide based on the sea buckthorn seed meal protein, wherein the protease comprises alkaline protease, compound protease, neutral protease, bromelain and papain, and the invention aims to excavate the development prospect of the sea-buckthorn seed meal active peptide and expand the application potential of the sea-buckthorn seed meal active peptide in different disease fields.

The invention discloses a liver protection composition containing glutathione-rich yeast. The liver protection composition is prepared from the glutathione-rich yeast, turmeric, silybum marianum, enzymolysis polypeptide with homology of medicine and food and anti-inflammatory and anti-oxidation auxiliary components. Wherein the medicine and food homologous enzymolysis polypeptide is prepared from radix puerariae through neutral protease-cellulase double enzymolysis, the polypeptide sequence is identified as Leuu-Val-Glu-Pro-Ala, and the medicine and food homologous enzymolysis polypeptide has the capacity of remarkably inhibiting LPS-induced RAW264.7 cell NO release and removing DPPH free radicals. According to the composition, through multi-component synergism, in an anti-alcohol experiment, the blood alcohol concentration decline rate of a mouse can be remarkably increased, the MDA content of liver tissue is remarkably reduced, and no acute toxic reaction exists. The composition has the functions of protecting the liver and dispelling the effects of alcohol, is safe and effective, and is suitable for the field of health-care products or functional foods.

The invention relates to the technical field of food biology, and discloses an antiviral cubilose polysaccharide active component enriched with bound sialic acid as well as a preparation method and application of the antiviral cubilose polysaccharide active component. The weight-average molecular weight of the cubilose polysaccharide active component is 30-36 kDa, the molecular weight distribution coefficient is 1.1-1.2, the content of bound sialic acid is greater than or equal to 24.0%, and the content of total sugar is greater than or equal to 57.0%. The preparation method comprises the following steps: pretreating a cubilose raw material in a boiling water bath, and sequentially carrying out alkaline protease and neutral protease enzymolysis; carrying out alcohol precipitation on supernate, redissolving, and carrying out papain enzymolysis; and after enzyme deactivation, adjusting the ethanol concentration to 75-85%, precipitating, and drying. According to the method, the cubilose polysaccharide can be efficiently enriched, the combined sialic acid structure of the cubilose polysaccharide is reserved, and the obtained component has the effects of resisting oxidation, inhibiting pathogenic microorganisms and regulating intestinal microecology.

The invention discloses a preparation method of a crocodile polypeptide extract and a beverage, and relates to the technical field of polypeptide extraction and beverage preparation, and the preparation method comprises the following steps: pretreatment of crocodile skin; denaturation treatment: adding a NaHCO3 buffer solution, and treating under a microwave condition; performing ultrasonic pretreatment; carrying out enzymolysis reaction, namely sequentially carrying out primary enzymolysis of alkaline protease and secondary enzymolysis of compounding trypsin and neutral protease; adding composite colloid after enzyme deactivation treatment, wherein the composite colloid comprises flaxseed gum and xanthan gum; centrifuging to take supernate, and carrying out fractional separation through ultrafiltration membranes with the molecular weight cut-off of 500Da and 1000Da; and concentrating and drying to obtain polypeptide powder. According to the method, the polypeptide yield, the beverage stability and the oral bioavailability are improved; finally, the obtained polypeptide is scientifically matched with hyaluronic acid and vitamin C to prepare the beautifying beverage with the effects of remarkably improving skin stains and fine wrinkles and brightening the skin color, and the breakthrough of high-value utilization of crocodile skin resources and product function integration is realized.

The invention relates to the technical field of daily chemicals, in particular to a color-protecting laundry detergent containing plant extracts and a preparation method of the color-protecting laundry detergent. The color-protecting laundry detergent is prepared from the following components in percentage by mass: 12.5 to 18.3 percent of natural anionic surfactant, 6.4 to 9.7 percent of nonionic surfactant, 3.2 to 5.8 percent of plant polyphenol extract, 3.0 to 5.2 percent of fiber affinity natural polymer, 2.1 to 4.0 percent of bio-based chelating aid, 1.8 to 3.5 percent of natural glycoside synergist, 0.9 to 2.3 percent of pH buffering agent, 0.6 to 1.4 percent of antioxidant plant essential oil and the balance of deionized water. The compound protease comprises alkaline protease, neutral protease and papain; the invention aims to solve the technical problems that the existing laundry detergent is poor in color protection effect, insufficient in environmental protection performance and large in damage to fabrics, and provides a laundry detergent product which takes natural plant extracts as core components and has an efficient color protection function.

This invention belongs to the field of alcoholic beverage processing technology, specifically relating to a high-purity ginkgo wine and its preparation method. The preparation method comprises the following steps: ginkgo slurry undergoes preliminary enzymatic hydrolysis with neutral protease and amylase, followed by secondary enzymatic hydrolysis with pectinase and sodium bisulfite; brewing yeast fermentation broth is added for pre-fermentation; glutamine transaminase, *Saccharomyces cerevisiae* fermentation broth, and *Candida valerate* fermentation broth are added for post-fermentation; and finally, distillation and filtration are performed to obtain the ginkgo wine. This invention achieves complete decomposition and fermentation of ginkgo kernels through a two-step enzymatic hydrolysis and two-step fermentation process. The preparation method has advantages such as simple process and low cost, which is beneficial for industrial production; the prepared ginkgo wine has advantages such as clarity, strong aroma, high alcohol content, and low acidity.

The present application relates to the technical field of small molecule peptide preparation, and specifically discloses a preparation process of selenium-rich antioxidant pig lung polypeptide. The preparation process of the selenium-rich antioxidant pig lung polypeptide comprises the following steps: washing and decontaminating fresh pig lung, peeling off the trachea, removing grease, and twisting into a minced meat shape to obtain pig lung pre-preparation material; mixing the pig lung pre-preparation material with a vitamin C aqueous solution, adding nano-silicon dioxide powder and eugenol to the mixture, and performing ultrasonic treatment; cooling to-10 to-30 DEG C, keeping for 1 to 2 hours, and then increasing the temperature to room temperature; homogenizing treatment, filtration, collecting the precipitate, and obtaining pretreated pig lung; adding water to the pretreated pig lung and stirring until uniform; adjusting the pH value of the system; adding a stabilizing alkaline protease for treatment; adjusting the system to be neutral; adding a neutral protease for enzymolysis; and cooling to room temperature to obtain pig lung enzymolysis liquid; centrifuging the pig lung enzymolysis liquid, ultrafiltering, collecting the permeate, nanofiltrating, collecting the concentrated liquid, and drying.

The invention discloses a sea-buckthorn small molecule peptide and a preparation method thereof, and relates to the field of biological medicine and food processing. According to the method, sea-buckthorn pulp residues and sea-buckthorn seed residues are used as composite raw materials and are mixed and stirred to form homogenate, and then cell wall structures are destroyed through ultrasonic wall breaking treatment to release protein components; then cutting off a peptide bond under the action of compound enzymes (plant protease, neutral protease and pepsase) by adopting an ultrasonic-enzyme synergistic hydrolysis process to generate a small molecule peptide fragment; after removing impurities through centrifugal separation, purifying through an ultrafiltration membrane (the molecular weight cut-off is 1-3kDa) to obtain a small molecule peptide solution; and performing debitterizing treatment by using macroporous adsorption resin, and preparing sea-buckthorn small molecule peptide powder by using a vacuum freeze-drying technology. According to the method, through ultrasonic-enzyme synergistic interaction, multi-stage purification combination and low-temperature keep-alive processes, the yield, molecular weight uniformity and antioxidant activity of small molecular peptides are remarkably improved, and high-valued utilization of sea-buckthorn processing by-products is achieved.

The invention discloses uric acid-reducing peptide goat milk powder and a preparation method thereof, and belongs to the technical field of enzymes. According to the process for preparing the uric acid lowering peptide by hydrolyzing goat milk through the compound enzyme, the total addition amount of the compound enzyme (the enzyme activity ratio of neutral protease N to neutral protease X is 1: 1) is 4000 U / g-6000 U / g, enzymolysis is conducted for 2-3 h at the temperature of 45-55 DEG C, and under the condition, the xanthine oxidase inhibition rate is 71.67 + / -1.32%-83.64 + / -0.98%. The enzymolysis goat milk containing the uric acid-reducing peptide is subjected to spray drying to prepare the uric acid-reducing peptide goat milk powder, and the storage stability of the goat milk powder is relatively good. The amino acid sequence and the source of the uric acid lowering peptide are separated and identified, the in-vitro uric acid lowering activity of the uric acid lowering peptide is verified through the solid-phase synthesized uric acid lowering peptide, and a theoretical basis and technical support are provided for development of new uric acid lowering peptide goat milk products.