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Method for preparing collagen peptide from fish scales

A technology of collagen peptides and fish scales, which is applied in the field of preparation of collagen peptides from fish scales, can solve problems such as limited application and difficulty in decompression of collagen peptides, and achieve the goal of increasing extraction rate, increasing peptide content and purity, and reducing enzymatic hydrolysis time Effect

Inactive Publication Date: 2012-12-26
WENZHOU UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Fish scale collagen peptides extracted with protease generally have a bitter taste, which limits its application in food, etc., and the debittering of collagen peptides is relatively difficult

Method used

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  • Method for preparing collagen peptide from fish scales
  • Method for preparing collagen peptide from fish scales
  • Method for preparing collagen peptide from fish scales

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1: Fish scale pretreatment

[0029] The scales are freshwater mixed fish scales, mainly crucian carp scales collected from the vegetable market. They are washed with tap water and then soaked in hydrochloric acid for decalcification. After decalcification, they are washed with tap water to neutrality, and then dried at 60°C (24~48 h ) Then crush it with a crusher.

[0030] Analyze the appropriate material-to-liquid ratio, hydrochloric acid concentration and decalcification time (the time when the calcium concentration in the soaking solution becomes stable) for hydrochloric acid decalcification by detecting the calcium concentration of the soaking solution. The calcium concentration is determined by EDTA titration.

[0031] The experimental results under different experimental conditions are as follows:

[0032] Table 1 Experimental results of fish scale decalcification under different experimental conditions

[0033] Experiment number1234 The ratio of scale and hy...

Embodiment 2

[0035] Example 2: Extracting fish scale collagen peptides by mixing enzymes

[0036] Take the decalcified, dried and pulverized fish scales prepared in Experiment 2 of Example 1 into the enzyme reactor, and then add water and mixed protease for mixed enzyme extraction of fish scale collagen peptides. After the mixed enzyme extraction is completed, the enzymes are inactivated and centrifuged at 4000 rpm for 10 minutes Afterwards, the enzymolysis solution is obtained, in which the mixed protease is a mixture of neutral protease, papain and flavor protease. The specific steps of inactivating the enzyme are: heating the enzymatic hydrolysate to above 90°C for 10 minutes.

[0037] The extraction rate of fish scale collagen peptides, the degree of hydrolysis and the amount of free amino acids in the enzymatic hydrolysis solution were used as the detection and analysis indicators. During the extraction process, when the extraction rate of fish scale collagen peptides in the solution tend...

Embodiment 3

[0057] Example 3: Membrane concentration of fish scale collagen peptides

[0058] The enzymatic hydrolysate prepared in Experiment 3 of Example 2 was centrifuged at 4000 rpm for 10 minutes to obtain the supernatant, and then the filtrate was concentrated in the combined membrane equipment. The primary membrane is a roll membrane with a molecular weight of 2000Da, which is carried out at room temperature and 1.0-1.5Mpa. The secondary membrane is a roll membrane with a molecular weight of 400Da, which is carried out at room temperature and 2.5~3.0Mpa. The secondary membrane is concentrated and freeze-dried to obtain fish scale collagen peptide powder.

[0059] Table 3 shows the yield of collagen peptides concentrated by the secondary membrane, and Table 4 shows the peptide content, molecular weight distribution and bitterness of the collagen peptide powder.

[0060] The yield of membrane-concentrated collagen peptides (%)=total hydroxyproline after concentration / total hydroxyproline ...

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PUM

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Abstract

The invention discloses a method for preparing collagen peptide from fish scales, which is characterized by comprising the following steps of: 1, taking the fish scales and immersing hydrochloric acid to remove calcium; washing to be neutral, drying and crushing; 2, adding the calcium-removed, dried and crushed fish scales obtained by the step 1 into an enzyme reactor; adding water and mixed protease to extract the collagen peptide; after the extraction, carrying out enzyme deactivation and centrifuging to obtain an enzymolysis solution, wherein the mixed protease is a mixture of neutral protease, papain and flavored protease; and 3, carrying out film concentration on the enzymolysis solution obtained by the step 2; and freezing and drying a concentrated solution to obtain fish scale collagen peptide powder. According to the fish scale collagen peptide obtained by the invention, the neutral protease, the papain and the flavored protease are used for extracting the fish scale collagen peptide by an enzymic method at one step, so that not only can the enzymolysis time (9 hours)be reduced, but also the extracting rate (72.72%) of the collagen peptide is improved, and solves the special flavor problem of bitter taste of the collagen peptide and the like.

Description

Technical field [0001] The invention belongs to the field of preparation of collagen peptides, and specifically relates to a method for preparing collagen peptides from fish scales. Background technique [0002] Collagen is widely found in animals, accounting for about 30% of the total protein in animals. It plays an extremely important role in the mechanical protection of tissues and organs and the regulation of the cellular microenvironment. The types of collagen are more complicated. There are about 27 reported types. The more common types are type I, type II, type III, type IV, and type V, among which type I collagen has the most application value. Type I collagen has a wide range of applications in food processing and packaging, biomedical materials, pharmaceuticals, leather, papermaking, imaging, biosynthesis and beauty and skin care products, especially in the application of beauty skin care and biomedical materials develop rapidly. [0003] Fish scales account for 1-5% of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06
Inventor 周茂洪赵肖为
Owner WENZHOU UNIVERSITY
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