2676 results about "Enzymatic hydrolysis" patented technology

Compositions and methods for biomass conversion are provided. Compositions comprise novel enzyme mixtures that can be used directly on lignocellulose substrate. Methods involve converting lignocellulosic biomass to free sugars and small oligosaccharides with enzymes that break down lignocellulose. Novel combinations of enzymes are provided that provide a synergistic release of sugars from plant biomass. Also provided are methods to identify enzymes, strains producing enzymes, or genes that encode enzymes capable of degrading lignocellulosic material to generate sugars.

The invention is directed to a pharmacologically active peptide conjugate having a reduced tendency towards enzymatic cleavage comprising a pharmacologically active peptide sequence (X) and a stabilising petide sequence (Z) of 4-20 amino acid residues covalently bound to X.

A process for the treatment of biomass to render structural carbohydrates more accessible and/or digestible using concentrated ammonium hydroxide with or without anhydrous ammonia addition, is described. The process preferably uses steam to strip ammonia from the biomass for recycling. The process yields of monosaccharides from the structural carbohydrates are good, particularly as measured by the enzymatic hydrolysis of the structural carbohydrates. The monosaccharides are used as animal feeds and energy sources for ethanol production.

A method comprising selection of unbroken whole grain rice that are first washed, or whole grain corn that is first reduced in size, and then making an aqueous slurry that is subsequently wet milled to release all the protein, fat, fiber, and starch components normally held in the structure of the grain. The resulting slurry can be reacted with heat to gelatinize the starch and the subsequent product dried. Also, the heated slurry containing the liberated components can be treated to enzymatic hydrolysis via the process of liquefaction and optionally saccharification, producing whole grain rice milk products having diverse carbohydrate compositions. The whole grain milk products are characterized by a nutritional composition containing substantially all the nutritional components of the whole grain, being an opaque whole milk colloid, having smooth texture versus pulpiness, lacking in all bitterness normally associated with whole grain products, and having a variety of sweetness levels from non-sweet to very sweet.

The present invention relates to the production of a highly digestible, high protein product (high protein distillers dried grains or high protein DDG) from corn endosperm, and more particularly to a method for the recovery of high protein DDG by using: (i) dehulling and degermination to isolate a low fat, low fiber corn endosperm fraction, (ii) enzymatic hydrolysis to solubilize and alcoholic fermentation to assimilate the starch and non-starch carbohydrates present in the corn endosperm, and (iii) filtration and/or centrifugation to recover the dealcoholized insoluble solids that remain after fermentation of the corn endosperm. The present invention provides an alternative to the traditional dry mill method of processing corn to produce ethanol, and results in the production and recovery of a distillers' by-product (high protein DDG) with increased value and range of use as an ingredient in feeds for farm-raised ruminants and non-ruminants and pet foods. The product of the present invention contains less than about 2.0 weight percent starch, from about 55.0 to about 65.0 weight percent protein, from about 4.5 to about 7.5 weight percent fat, from about 3.0 to about 5.0 weight percent crude fiber, and from about 78.0 to about 90.0 percent total digestible nutrients, and improves the palatability and digestibility of animal feeds and/or pet foods into which it is incorporated, and aids in the management of the health and weight gain of the animal.

A process for producing cellulolytic and/or hemicellulolytic enzymes uses the residue from the ethanolic fermentation of enzymatic hydrolyzates of cellulosic or ligno-cellulosic materials. This process may be integrated into a process for the production of ethanol from cellulosic or ligno-cellulosic materials which comprises the following steps: 1) chemical and/or physical pre-treatment of a cellulosic or ligno-cellulosic substrate; 2) enzymatic hydrolysis of the pre-treated substrate using cellulolytic and/or hemicellulolytic enzymes; 3) ethanolic fermentation, by a suitable alcohologenic microorganism, of the hydrolyzate from step (2) and production of a fermentation must; and 4) separation of the alcohologenic microorganism used in step (3), separation/purification of the ethanol and production of an aqueous phase constituting a residue; and in which said residue serves for the production of the cellulolytic and/or hemicellulolytic enzymes used in step 2).

The present invention relates to the production of a highly digestible, mid-level protein DDGS from corn endosperm, and more particularly to a method for the recovery of mid-level protein DDGS by using: (i) dehulling and degermination to isolate a low fat, low fiber corn endosperm fraction, (ii) enzymatic hydrolysis to solubilize and alcoholic fermentation to assimilate the starch and non-starch carbohydrates present in the corn endosperm, and (iii) filtration, centrifugation and/or evaporation to recover the dealcoholized insoluble and soluble solids that remain after fermentation of the corn endosperm. The product of the present invention contains less than about 5.0 weight percent starch, from about 40.0 to about 52.5 weight percent protein, from about 4.5 to about 8.5 weight percent fat, from about 3.0 to about 6.0 weight percent crude fiber, and from about 78.0 to about 90.0 percent total digestible nutrients.

A process is defined for the continuous steam pretreatment and fractionation of corn cobs and low lignin lignocellulosic biomass to produce a concentrated cellulose solid stream that is sensitive to enzymatic hydrolysis. Valuable chemicals are recovered by fractionating the liquid and vapor stream composed of hydrolysis and degradation products of the hemicellulose. Cellulosic derived glucose is produced for fermentation to biofuels. A hemicellulose concentrate is recovered that can be converted to value added products including ethanol.

The invention relates to a preparation method of a walnut peptide powder, which effectively embodies a plurality of biological functional properties of the walnut peptide powder, reserves a plurality of nutritional components of walnuts. The polypeptide content in the walnut peptide powder is 60-80 percent, while the walnut peptide powder is light yellow or yellow-white color and has functions of anti-fatigue and antioxidation, etc. The invention comprises the following operation steps: crushing the walnut cakes before extracting the walnut protein, then carrying out enzymatic hydrolysis and centrifugation to the crude walnut protein to acquire the supernatant, dialyzing the supernatant and removing impurities and macromolecular compounds, and then concentrating the acquired dialysate, and at last vacuum drying to prepare the walnut peptide powder and packing into finished products.

Provided is an enzyme mixture for hydrolyzing a pretreated lignocellulosic feedstock to soluble sugars. The enzyme mixture comprises EG4 at a fractional concentration (f_EG4) of about 0.25 to about 0.83 (w/w), Swollenin at a fractional concentration (f_Swo1) of about 0 to about 0.66 (w/w), and Cip1 at a fractional concentration (f_Cip1) of 0 to about 0.33 measured relative to all accessory enzymes present in the enzyme mixture. Also provided are processes for converting a pretreated lignocellulosic feedstock to soluble sugars using the enzyme mixtures, and methods of using and producing such enzyme mixtures.

A process for the enzymatic hydrolysis of cellulose to produce a hydrolysis product from a pre-treated cellulosic feedstock is provided. The process comprises introducing an aqueous slurry of the pre-treated cellulosic feedstock at the bottom of a hydrolysis reactor. Axial dispersion in the reactor is limited by avoiding mixing and maintaining an average slurry flow velocity of about 0.1 to about 20 feet per hour, such that the undissolved solids flow upward at a rate slower than that of the liquid. Cellulase enzymes are added to the aqueous slurry before or during the step of introducing. An aqueous stream comprising hydrolysis product and unhydrolyzed solids is removed from the hydrolysis reactor. Also provided are enzyme compositions which comprise cellulase enzymes and flocculents for use in the process. In addition, a kit comprising cellulase enzymes and flocculent is provided.

The invention relates to a method of extracting hemicellulose, cellulose and lignin from wood fiber raw materials and belongs to the field of utilization and technology of biomass resources of agriculture and forestry. In the method, the hemicellulose is extracted from the wood fiber raw materials by using hot water, then the lignin is extracted by using alkaline organic solvent under mild conditions; and residual ingredients rich in the cellulose are further used for enzyme hydrolysis to prepare fermentable sugar solution, thereby achieving full-ingredient utilization of the hemicellulose, cellulose and lignin in the biomass resources of the agriculture and forestry. The method has simple process, and is environmentally friendly, the organic solvent can be recycled, thereby promoting the development of the green agriculture and forestry, and the method has wide social and economic benefits.

The invention relates to a method for recovering energy from the organic fraction of urban solid waste comprising the following steps: a) the organic fraction is pre treated with mineral acids, preferably sulfuric acid, during which the fraction is heated by an outer thermal jacket with no steam injection or steam explosion, thereby producing a first slurry containing an insoluble solid susceptible to enzymatic attack by cellulases; b) a step comprising enzymatic hydrolysis using cellulases and simultaneous fermentation, using an ethanologenic microorganism, of the first slurry in order to obtain a second slurry containing diluted ethanol; and c) distillation of the second slurry such as to obtain wet ethanol, a recyclable liquid effluent and a solid.

The invention relates to a method for natural dried fruit essence by utilizing dried fruit raw materials, which comprises the following steps: taking peanut meal, cocoa powder, coffee powder and other dried fruit matters as raw materials; firstly extracting and concentrating flavor substances easier for volatilization in the raw materials by utilizing a natural extraction, resin adsorption and solvent desorption technology to obtain aqueous essence A; then drying and baking the raw materials remaining in the previous step, extracting with an organic solvent after fragrance is newly generated to obtain oily essence B; successively carrying out enzymatic hydrolysis on the remaining raw materials, centrifugally removing and precipitating, mixing with amino acid and reducing sugar for Maillard reaction to obtain pasty essence C; and finally spray-drying a precipitate after being subjected to enzymatic hydrolysis to obtain powdered flavor. Through all the steps, a series of dried fruit essence with different states which can be applied to different products can be obtained. The method has the advantages that the process is simple, the industrial production is convenient, the raw materials are fully utilized, the production cost can be reduced helpfully, and the fragrance is round, full, rich and natural.

The invention discloses a method for one-time separation and extraction of dendrobe polysaccharide, dendrobine and erianin from dendrobium candidum. The method for extraction of dendrobe polysaccharide, dendrobine and erianin from dendrobium candidum comprises the steps of crushing of dendrobe, enzymatic hydrolysis, ultrasonic extraction, microfiltration, ultrafiltration and nano-membrane concentration, solid-liquid separation, vacuum cryogenic distillation, solvent extraction, solvent recovery, recrystallization, vacuum low-temperature drying and purification in the carbon dioxide supercritical fluid, thereby obtaining the dendrobe polysaccharide, dendrobine and erianin. The prepared product has good color, good solubility and high active ingredients.

A biorefining process used to convert lignocellulosic biomass into ethanol via a fermentation pathway. In a first pretreatment process step, the biomass is mixed with an aqueous wash solution to remove soluble carbohydrates from the biomass structure. Next, the solid fraction is separated from a liquid fraction. In a second pretreatment process, the solid fraction is pre-treated to make the fiber bundles and complex polysaccharides more amenable to enzymatic hydrolysis. Following the second pretreatment process, the pre-treated biomass is subjected to one or more enzymes in a hydrolysis process. The liquid fraction isolated from the first pretreatment process is diverted past the second pretreatment process and is recombined with the solid fraction in the hydrolysis process. The enzyme cocktail in the hydrolysis process breaks down the alpha- and hemicellulose polymers into fermentable sugars. Finally, a fermentation process produces a “beer” that is further processed in a distillation and dehydration process.

The invention relates to a method for making a pet food attractant, which takes duck liver and marine fish as a main material, wherein the addition of the duck liver is 50-100%. The method comprises the following steps: mincing the raw material by a mincer, adding a proper amount of water for disinfection, adding protease for enzymatic hydrolysis, adding amino acid and carbohydrate for carrying out maillard reaction after the enzymatic hydrolysis reaction is finished, spray drying to obtain the food attractant specially used for pet, wherein the water content of the finished product is 6-10%.According to the enzymatic hydrolysis method in the invention, the partial protein is degraded to a polypeptide substance which is easy to be absorbd by pet, The specific local flavor food attractant is prepared by controlling the maillard reaction condition and the food attractant product is finally prepared, the food attractant with dark red color has rich meat fragrance, thereby the initiative for ingestion of pet can be enhanced for first time. The food attractant is capable of applying to exploitation of various pet principal food and snacks products.

A process for increasing production of sugars from cellulose in a plant biomass using ammonia after swelling of the biomass with water and enzymatic hydrolysis is described. The sugars are preferably fermented to an alcohol, particularly ethanol as a fuel for vehicles.

The invention relates to a processing method of sea cucumber glycopeptides chelated calcium. The method comprises the following steps: A. material treatment: cleaning sea cucumber mouthparts, cooking, soaking, beating, and homogenizing; B. biological enzymatic hydrolysis: choosing a variety of protease for composite segmented enzymatic hydrolysis; C. centrifugal separation: conducting continuous centrifugal separation on an enzymolysis liquid to obtain the sea cucumber glycopeptides solution and sea cucumber calcium precipitate; D. filtration, concentration and grading: subjecting the enzyme liquid to ultrafiltration fractionation concentration, and desalinating small peptide filtered solution by nanofiltration; E. preparation of sea cucumber calcium by organic acidolysis; G. chelating reaction: promoting the chelating of sea cucumber calcium and glycopeptides of sea cucumber by microwave assistance; and H. after the reaction, carrying out centrifuging separation, and drying the precipitate and supernatant respectively. The present invention comprehensively utilizes the sea cucumber processing waste to prepare sea cucumber autologous calcium and autologous glycopeptides for chelation. The method on the one hand is beneficial to playing their biological effects, and on the other hand can also obtain five products with different specific biological functions.

The invention provides an enzymolysis lignin epoxy resin and a preparation method thereof. The raw materials include: an enzymolysis lignin-dianhydride prepolymer, a glycidol ether, a catalyst, a curing agent and a filler. The preparation method comprises the following steps that: under the action of the catalyst, an etherification of a hydroxyl group contained in the enzymolysis lignin or a derivative of the enzymolysis lignin and dianhydride is carried to synthesize a prepolymer of enzymolysis lignin-dianhydride; then the prepolymer reacts with the glycidol ether and undergoes etherification and ring closing reaction to obtain an enzymolysis lignin--polyester type epoxy resin. The enzymolysis lignin adopted by the method is natural macromolecular material extracted from the residues which is produced in the preparation of alcohol through the fermentation of wood chips, bamboo and stalk, thereby not only effectively utilizing biological resources, but also replacing petrochemical raw materials. The enzymolysis lignin epoxy resin which can be used as a modifier for engineering materials, adhesives, paints and macromolecular materials has a broad application prospect.

The invention relates to a cereal yoghurt and a preparation method thereof. The present invention uses various coarse grain powders as raw materials, undergoes amylase hydrolysis process to make hydrolyzed grain slurry and then mixes them with fresh milk or milk powder, or mixes various coarse grain powders etc. with fresh milk or milk powder and then adds amylase Hydrolyzed and then fermented by lactic acid bacteria to produce a unique flavor of coarse grain yogurt, and the content of coarse grains in the final product can reach 1-40%. The invention belongs to a new type of dairy product, which not only has a unique taste, but also contains milk High-quality protein, also contains sufficient carbohydrates and a variety of micronutrients, can be used as a good meal replacement food or dessert.

The invention provides a milk white fructus momordicae extract, fructus momordicae glycoside V and fructus momordicae glycoside of which are respectively larger than or equal to 40 percent and 98 percent. The invention also discloses a preparation method of the extract. Using fresh fructus momordicae as the raw material, the invention is obtained through the water extraction, the concentration, the enzymatic hydrolysis, the macroporous adsorption resin refining, the ion exchange resin decoloration, the concentration and the spray drying. The invention is characterized in that the process parameters of the steps which are suitable for the industrial production are found, the yield and the quality of the fructus momordicae extraction are all obviously improved, and obvious economic benefits and social benefits are obtained.

The invention describes a process for preparing acetone starting from acetyl-coenzyme A comprising process steps A. enzymatic conversion of acetyl-CoA into acetoacetyl-CoA B. enzymatic conversion of acetoacetyl-CoA into acetoacetate and CoA and C. decarboxylation of acetoacetate to acetone and CO₂, which is characterized in that the coenzyme A is not transferred in process step B to an acceptor molecule. In addition, process step B is surprisingly catalysed by enzymes of the classes of acyl-CoA thioesterase, acyl-CoA synthetase or acyl-CoA thiokinase.

A completely novel metabolic pathway is concerned, because the enzymatic hydrolysis of acetoacetyl-CoA without simultaneous transfer of CoA to a receptor molecule has never previously been described for any microbial enzyme.

The present invention discloses a production method of functional Chinese wolfberry fruit enzyme and product thereof. The production method includes pulping Chinese wolfberry fruits, conducting enzymatic hydrolysis, adjusting the solid content and pH value, sterilizing, inoculating probiotics and fermenting, conducting centrifugal separation, drying the thick slurry to obtain the Chinese wolfberry fruit enzyme powder, continuously inoculating the clear juice with probiotics and fermenting to obtain the Chinese wolfberry fruit enzyme stock solution, and after-ripening to obtain the Chinese wolfberry fruit enzymes. And the enzymes obtained can be used to produce other Chinese wolfberry fruit enzyme products including Chinese wolfberry fruit enzyme beverage, Chinese wolfberry fruit enzyme powder, effervescent tablets, chewable tablets, buccal tablets, granules, capsules, etc. The products have efficacies of clearing heat and toxins, replenishing blood, improving eyesight, lowering blood lipids and losing weight, improving body oxidation resistance, regulating balance of intestinal floras, enhancing immunity, promoting beauty, facilitating sleep, and preventing aging.

The invention discloses a preparation method for plant/fruit/vegetable enzymes. The method mainly comprises the following steps: (1) preparing plant/fruit/vegetable juice; (2) carrying out enzymatic hydrolysis; (3) carrying out first-time fermentation; (4) carrying out second-time fermentation; (5) carrying out dipping. According to the preparation method provided by the invention, the fermentation cycle is short, and fermented raw materials can be decomposed thoroughly, so that the content of substances related to flavor and taste in the plant/fruit/vegetable enzymes is increased, the content of nutrients is higher, and the absorption is easier; through repeated sterilization processes, the stability of the plant/fruit/vegetable enzymes is guaranteed.

The invention relates to a maize oligopeptide and a method for preparing same, belonging to the field of biological technology. The method takes the byproduct, namely corn protein powder (also called maize yellow powder containing about 60% of protein) in corn starch production as a raw material, removes the impurities such as starch, grease and the like by the treatment of alkaline water and obtains high-purity corn protein slag; furthermore, the corn protein slag is taken as enzymatic hydrolysis substrate and treated by the following process of pre-treatment, enzymatic hydrolysis, multi-step separation and purification, concentrating, drying and the like so as to prepare the maize oligopeptide powders with edible value and various health-care functions. The method for preparing maize oligopeptide has the advantages of easily obtaining raw material, simple process and higher industrial feasibility. The prepared maize oligopeptide sample has high crude protein content and small molecular weight, has various physiological activities of prompting immunity adjustment, anti-fatigue and the like, and can be used as new functional nutritional compounding and widely applied to the development and production of products such as foods, health-care foods and the like.

The invention relates to a method for preparing wheat bran dietary fiber by utilizing ultrasound-assisted enzymatic hydrolysis, and in particularly belongs to the technical field of the agricultural and sideline product development, the functional food additive and the nutritional heath caring. The invention is characterized in that the invention adopts wheat bran as the raw material, and the wheat bran dietary fiber preparation is realized through utilizing the ultrasound treatment, and removing starch and protein by enzymatic method, and then the preparation of fine dietary fiber with different grain size is realized by crumbling with a grinder. The invention realizes the effective value increment and the utilization of the wheat bran, the prepared functional dietary fiber has important physiological function, and thereby can realize the functions of preventing constipation, colon cancer, intestina diverticulum, hemorrhoid and varicose veins of the lower extremities; the serum cholesterol is lowered, and the heart disease caused by coronary sclerosis can be prevented; the sensitivity of the peripheral nerve tissue to the insulin is improved, and the blood sugar level of the diabetes patient can be regulated, the invention is very useful for human health, and the economic benefit as well as the social benefit are greatly increased.

The invention relates to an enzymatical extraction process of oil in tea seed oil kernel, which comprises the following steps: firstly, utilizing dried tea seed oil kernel to be raw material, immersing in water in high temperature after disintegrating, stirring in uniform speed under 70-100 DEG C, secondly, enzyme hydrolyzing, adding acid protease which is 0.02-1% dry weight of disintegration samples or mixed enzyme of acid protease/cellulose in 45-55 DEG C to stir in uniform speed, and enzymatic hydrolysis for 2-4 hours, thirdly, separation emulsion breaking processing, directly separating out primary free boiled oil after enzymolysis liquid is centrifuged, getting secondary free boiled oil through centrifuging after missible oil demulsifies, combining the primary free boiled oil and the secondary free boiled oil, and getting the total boiled oil. The invention has the advantages that first, the invention reduces the problem of emulsification, foaming and the like which are faced when a grinding method is utilized to destroy cell structures, second, the invention prevents various enzymes in cells from degrading oil, which has high oil extraction rate to a further disintegrated function to tea seed oil kernel cell, third, the invention increases the treatment to missible oil, which increases free boiled oil yield through demulsifying, centrifuging, stewing to layer and the other modes.

The invention discloses a preparation method of instant oat flour and relates to a preparation method of oat flour. The preparation method comprises the following steps: baking, cleaning, soaking, steaming and cooling oats, serving as raw materials, sequentially; glue milling the oats after pretreatment to prepare oat pulp, carrying out enzymatic hydrolysis, heating, boiling, and killing enzyme; and cooling an enzymolysis liquid, glue milling, homogenizing, drying, sterilizing and packaging a finished product. According to the preparation method disclosed by the invention, with the oats as the raw materials, by virtue of pretreatment, enzymatic hydrolysis technological treatment and spray drying, the instant oat flour which can keep multiple nutritional components and fragrance of oat is prepared; the prepared instant oat flour is moderate in taste, has self flavor of oat, keeps the soluble nutritional components in the oats, is also capable of providing extra plant proteins, fats and soluble dietary fibers and can be directly used for preparing an oat beverage; as macromolecular substances including starch, cellulose and proteins in the oats are hydrolyzed by virtue of an enzyme method, the viscosity of the starch of the oats is lowered, and the problems of water separating, gelation, precipitation and the like caused when the oats are applied to beverages are solved.

The invention relates to a method for producing a high-concentration reduced sugar by combining enzymatic hydrolysis of lignocellulose and membrane separation. In the method, a lignocellulose substrate, a hydrolytic enzyme and a reduced sugar hydrolysate in enzymolysis solution are separated through microfiltration and ultrafiltration, the repeated use of the substrate and the enzyme is realized, the enzymolysis solution is concentrated by nanofiltering, and thus, the solution of the high-concentration reduced sugar is prepared. In the method, due to the combination of microfiltration, ultrafiltration and nanofiltration, the contamination of the lignocellulose substrate to an ultrafiltration membrane is effectively reduced, the inhibiting effect of the hydrolysate on enzymatic hydrolysis of the lignocellulose is avoided, the enzymatic efficiency is improved, the cost of enzymolysis is reduced, energy and water in a production process are saved, the concentration of the reduced sugar in the enzymolysis solution is increased, and the production of a high-concentration product through microbial fermentation is easy.