1342 results about "Enzymatic hydrolysis" patented technology

The invention discloses preparation of a whey protein peptide mixture with immune and muscle-building effects, and relates to the technical field of food processing and bioengineering. The neutral protease is adopted and the pH is adjusted, so that the enzymolysis efficiency is effectively improved, and the enzymolysis time is greatly shortened; meanwhile, the whey protein peptide which is uniform in molecular weight distribution, free of peculiar smell and good in thermal stability is successfully prepared by accurately controlling enzymolysis conditions and combining microbial fermentation. The protein peptide can effectively improve thymus indexes and spleen indexes, enhance the proliferation capacity of splenic lymphocytes and the activity of NK cells, play an excellent immune regulation role and have a remarkable muscle building effect. The preparation process has the advantages of high efficiency, low cost, simplicity, convenience and the like, gives consideration to high quality and high functionality of the product, and has great application value.

The invention relates to a method for detecting activity of gamma-polyglutamic acid hydrolase and application. The method comprises the following steps: (1) preparing an enzymolysis reaction solution and a blank reaction solution; (2) preparing an NAC / OPA mixed solution; (3) adding an NAC / OPA mixed solution into the enzymatic hydrolysis reaction solution to obtain a reaction solution to be detected; (4) drawing a standard curve by taking L-glutamic acid as a standard substance; and (5) detecting the fluorescence intensity of the blank reaction solution and the reaction solution to be detected by using a microplate reader, and then calculating the activity of the gamma-polyglutamic acid hydrolase according to the fluorescence intensity and the standard curve. The method for detecting the activity of the gamma-polyglutamic acid hydrolase is high in detection speed, detection of a single sample can be completed within 25 min, and nearly real-time process monitoring is achieved. And the method has the advantages of reliable detection result and high accuracy, can be used for monitoring the enzyme activity of the gamma-polyglutamic acid hydrolase in the gamma-polyglutamic acid preparation process in real time and guiding the optimization of the fermentation process, and has extremely high industrial application value.

The invention discloses a preparation method and application of yak blood peptide with anti-hypoxia and anti-fatigue functions. The method comprises the steps of raw material pretreatment, composite enzymolysis (adopting a mixture of enzyme digestion modified lumbrukinase and nattokinase), enzyme deactivation, separation and purification, ferrous chelation, Maillard reaction, concentration and drying and the like. The enzymolysis efficiency and the active peptide yield are remarkably improved through ultrasonic treatment and a composite enzymolysis technology, the stability and the anti-hypoxia capacity of peptide fragments are enhanced through ferrous chelation, and the antioxidant activity and the anti-fatigue activity are further improved through the Maillard reaction. Experiments show that the yak blood peptide prepared by the method can significantly improve the antioxidant enzyme activity, prolong the normal-pressure hypoxia survival time, prolong the swimming exhaustion time and significantly reduce the MDA content of brain tissue and myocardial tissue. The obtained product can be applied to anti-hypoxia and anti-fatigue medicines or health-care foods, and has the advantages of high process efficiency, remarkable activity, high safety and the like.

The invention provides a lipidosome STING agonist delivery system based on a PD-L1 antibody as well as a preparation method and application of the lipidosome STING agonist delivery system, and belongs to the technical field of medical biology. Preparing lipidosome by adopting an ethanol injection method and an ammonium sulfate gradient technology; the PD-L1 antibody and the STING agonist can be loaded at the same time; the liposome is prepared by adopting an ethanol injection method and an ammonium sulfate gradient technology, and the STING agonist can be wrapped in the liposome in an active drug loading manner; dSPE-PEG2000-NHS and a PD-L1 antibody are mixed and incubated according to a specific proportion by utilizing a post-insertion method to form an antibody conjugated micelle, and the antibody conjugated micelle is fused with a blank liposome to realize antibody modification; secondly, the nano-liposome has good drug carrier characteristics and can effectively protect the loaded drug, reduce the risk of enzymolysis of the drug and improve the stability of the drug in vivo, so that the liposome drug delivery system simultaneously loading the nano-liposome and the nano-liposome is successfully prepared.

The invention discloses a preparation method of fresh taxus chinensis stem and leaf protoplasts. The preparation method comprises a preparation process and a purification process. The preparation method of the taxus chinensis stem and leaf protoplast is simple and easy to operate, raw materials are easy to obtain and low in price, reagent components have good biocompatibility, and the taxus chinensis stem and leaf protoplast is free of harmful components, safe and environmentally friendly. The invention provides a simple and rapid taxus chinensis stem and leaf protoplast enzymolysis preparation method, which adopts vacuum filtration, accelerates the permeation of enzymatic hydrolysate and improves the enzymolysis efficiency, so that the protoplast can be rapidly obtained. Meanwhile, high-purity separation of the protoplast is carried out in combination with density gradient sedimentation of the iodixanol solution, so that the protoplast with uniform size and complete form is obtained, and convenience is provided for scientific researches such as subsequent conversion and single cell transcriptome sequencing.

The invention relates to the technical field of scalp care, in particular to a hair-blacking composition for promoting melanin secretion and a preparation method of the hair-blacking composition. Comprising the following components in parts by weight: water, ethanol, a ginseng root extract, a polygonum multiflorum extract, a cacumen biotae extract, a licorice root extract, an eclipta prostrata extract, a ganoderma atrum extract, a rhizoma bletillae root extract, a mulberry leaf extract, radix sophorae flavescentis powder, a glossy privet fruit extract, a raspberry fruit extract and a black sesame extract. According to the technical scheme, the ganoderma atrum extract is prepared through enzymolysis-ultrasonic combined modification, a cell wall structure is decomposed through compound enzyme, then cell remains are further broken through the ultrasonic cavitation effect, and the release rate of active peptide and polysaccharide is increased; the hair-blacking composition can promote the generation of hair melanin so as to blacken hair, improve the hair quality, enable the hair to become tough and elastic, and enable the hair to gradually become black, smooth and firm after being smeared on the scalp for a long time.

The invention relates to the technical field of plant extraction, in particular to a multi-product preparation method based on an arabinoxylan-lignin compound. The method comprises the following steps: treating a lignocellulose raw material by adopting a weak base extracting solution and a strong base extracting solution, and separating an obtained strong base extracting and dealkalizing suspension into an arabinoxylan-lignin compound dispersion liquid and arabinoxylan; performing enzymolysis on the dispersion liquid, and filtering enzymatic hydrolysate to obtain a precipitate which is lignin and a filtrate which is xylooligosaccharide liquid; and separating and purifying the xylooligosaccharide liquid to obtain xylooligosaccharide, xylose and arabinose. According to the method, on the basis of a weak base-strong base two-step extraction process, obtained arabinoxylan-lignin compound dispersion liquid is subjected to enzymolysis, so that lignin, xylooligosaccharide, xylose and arabinose can be continuously prepared, and the reutilization of the arabinoxylan-lignin compound is realized.

The invention belongs to the technical field of food processing, and relates to a method for preparing low-salt shrimp juice through rapid microbial fermentation. The method comprises the following steps: removing heads of shrimps, preparing meat paste, drying and crushing shrimp shells, and mixing the shrimp shells with the meat paste; carrying out enzymolysis treatment on the meat paste by using alkaline protease, and then carrying out high-temperature enzyme deactivation; mixing the shrimp meat and shrimp shell enzymatic hydrolysate mixture with soybean meal and bran, and sterilizing at high temperature; inoculating a complex microbial inoculant containing lactobacillus plantarum, saccharomyces rouxii, aspergillus oryzae, deep-sea bacillus subtilis and bacillus subtilis to prepare a mold starter, fermenting at 28 DEG C for 30 days, and finally filtering and sterilizing. According to the method disclosed by the invention, the availability of the raw materials is effectively improved, the fermentation period is shortened, efficient fermentation under a low-salt condition is realized, various basic indexes of the obtained shrimp juice product meet national standards, and the shrimp juice product is outstanding in flavor quality and has rich sauce flavor and sweet taste. In addition, the shrimp juice shows good antioxidant stability before and after digestion.

The invention discloses a method for efficiently preparing a fatty acid type anti-wear agent based on biodiesel, which comprises the following steps: freezing and crystallizing the raw material biodiesel, filtering, evaporating and dealcoholizing, carrying out enzyme hydrolysis, insulating and layering, and rectifying under reduced pressure to finally obtain the fatty acid type anti-wear agent. Wherein the fatty acid composition of the crude methyl ester can be directionally regulated and controlled through freezing crystallization and filtering processes; through the synergistic effect of enzyme hydrolysis reaction and high-vacuum rectification separation coupling, efficient production of the fatty acid anti-wear agent prepared from biodiesel is achieved, the production period is greatly shortened, and the product quality is improved. According to the technology disclosed by the invention, the high-quality fatty acid type anti-wear agent can be produced economically, greenly, efficiently and stably, and the acid value of the product is stabilized between 198-203mgKOH / g oil and meets the Q / SHCG 57-2017 standard. The method has the advantages of low raw material requirement, wide application range, high production efficiency, high product quality, reproducible and reusable catalyst and the like, and is suitable for industrial production.

The invention relates to the technical field of food processing and making, and discloses a konjac food and a making method thereof, and the making method comprises the following steps: raw material pretreatment, swelling and dispersion, enzymolysis treatment, gel matrix preparation, flavor substance embedding, mixing and degassing, curing forming, ultrahigh pressure treatment and post-sterilization and packaging. In the invention, the konjac micro-powder is subjected to controllable enzymolysis through the compound bio-enzyme preparation, and the konjac micro-powder and the gel matrix liquid composed of carrageenan, gellan gum and curdlan gum in a specific ratio have a synergistic effect under a curing condition, so that the konjac colloid and the gel matrix are interwoven to form a compact and stable cementing system, and the texture of the konjac food is improved; according to the method, the plant-derived flavor extract is added, so that the taste of the plant-derived flavor extract is uniform and consistent, the defect that the traditional process product is unstable in texture or easily generates bad flavor is overcome, the plant-derived flavor extract is embedded by adopting beta-cyclodextrin, and the plant-derived flavor extract is converted into a microcapsule form through high-pressure homogenization and spray drying processes.

The invention relates to the technical field of film packaging, in particular to a high-barrier light-enzyme double-response controllable degradation three-layer composite film and a preparation method thereof.The preparation method comprises the steps that a base material layer and a functional layer are subjected to a film blowing process through a double-layer co-extrusion film blowing machine, a double-layer composite film is formed, after cooling, the surface of the double-layer composite film is coated with a barrier layer through a coating machine, and the double-layer composite film is obtained. The preparation method of the high-barrier light-enzyme double-response controllable degradation three-layer composite film provided by the invention is simple and low in cost, the high-barrier light-enzyme double-response controllable degradation three-layer composite film has high barrier property, rapid illumination and enzymolysis double-signal triggering is realized, rapid controllable degradation is realized, and the high-barrier light-enzyme double-response controllable degradation three-layer composite film has the advantages of high light-barrier property, high light-barrier property, high light-barrier property and high light-barrier property. The industrial problem that environmental protection and functionality are difficult to be compatible is solved.

The invention discloses a method for improving the quality of a Tieguanyin tea extracting solution through segmented composite enzymolysis extraction, and belongs to the technical field of tea drink preparation. The method comprises the following steps: S1, performing low-temperature aroma extraction on Tieguanyin dry tea, and performing crushing so as to obtain crushed tea; s2, adding water and beta-glucosidase into the broken tea to carry out first-stage enzymolysis extraction, and separating to obtain tea aroma extract liquid and aroma-free tea residues; s3, water and composite hydrolase are added into the aroma-free tea residues for second-stage enzymolysis extraction, separation and filtration are performed, and a hydrolysis extraction solution is obtained; and S4, mixing the tea fragrance extract liquid with the hydrolysis extract liquid, cooling, centrifuging and filtering to obtain the Tieguanyin tea extract liquid. According to the method disclosed by the invention, the original flavor of the Tieguanyin is maintained, meanwhile, the fragrance and the mouth feel are remarkably improved, and the problems of fragrance loss and bitter taste caused by traditional high-temperature long-time extraction and cooked soup taste generated by tannase enzymolysis are avoided.

The invention discloses an agaric polypeptide VR5 with alpha-glucosidase inhibitory activity and a preparation method and application thereof, and belongs to the technical field of small molecule peptides. The amino acid sequence of the auricularia auricula polypeptide VR5 is VGMLR, and the auricularia auricula polypeptide VR5 can be prepared by a solid-phase synthesis method and an enzymolysis method (flavourzyme). The agaric polypeptide VR5 has the beneficial effects that experiments prove that the agaric polypeptide VR5 has very high alpha-glucosidase inhibitory activity, IC50 is equal to 34.96 mu g / mL, the agaric polypeptide VR5 can be used as an alpha-glucosidase inhibitor for preventing or treating alpha-glucosidase mediated diseases, and the agaric polypeptide VR5 can be used for preventing or treating alpha-glucosidase mediated diseases. Or as a hypoglycemic active component, the composition is added into auxiliary hypoglycemic functional food for auxiliary regulation of the blood sugar level of hyperglycemia people.

The invention relates to the technical field of gel material preparation, in particular to a bio-based gel material for dangerous chemical leakage disposal and a preparation method thereof.The preparation method comprises the steps that gelatin and deionized water are added into a reaction container, a gelatin solution is obtained under the preset dissolution condition, alkaline protease is added into the gelatin solution for an enzymolysis reaction, and a gelatin solution is obtained; a modified gelatin solution is obtained; the preparation method comprises the following steps: adding a phytic acid-chitosan compound and magnesium-aluminum layered double hydroxide into dispersion equipment, and obtaining a composite inorganic matter dispersion liquid under a preset dispersion condition; adding the modified gelatin solution, the composite inorganic substance dispersion liquid, polyethylene glycol and phase change microcapsules into a mixing reactor, and obtaining a premixed solution under a preset mixing condition; sequentially adding an initiator, a cross-linking agent and tetramethylethylenediamine into the premixed liquid, and performing cross-linking reaction under preset cross-linking conditions to obtain a bio-based gel initial product. The plugging efficiency of the bio-based gel material on dangerous chemical leakage is improved.

The present application provides a kind of beverage with chickpea as plant protein source and a preparation method thereof.The protein in the prepared chickpea protein beverage is 100% or mainly derived from natural chickpea seeds.No stabilizer, thickening agent and sweetener is added in the product, and the above-mentioned purposes are achieved only by physical processing, enzyme hydrolysis and pH adjustment.The chickpea protein and starch components can be fully utilized, the fullness of the plant protein beverage is improved, and the chickpea protein beverage can still meet the commercial sterile requirements even under the condition of higher protein content.

The invention relates to the technical field of agricultural fertilizers, in particular to an enzymolysis fish protein type amino acid-containing water-soluble fertilizer and a preparation method thereof, the fertilizer is prepared from 50-70% of enzymolysis fish protein stock solution and other components; the stock solution is prepared by pulping aquatic product processing leftovers, adjusting the pH value to 6.5-7.5, adding specific compound protease for enzymolysis, and performing enzyme deactivation and centrifugation; the macroelement liquid contains nitrate nitrogen and ammonium nitrogen (2: 1-4: 1), and the microelement chelating agent is prepared by reacting a stock solution small molecule peptide with a specific salt. The fish protein enzymolysis stock solution is prepared from aquatic product processing leftovers, high-proportion small molecule peptide and amino acid are obtained through specific enzymolysis and other processes, and by matching with high-content macroelements in a specific nitrogen form, nutrition can be rapidly supplemented, and crop photosynthesis and dry matter accumulation can be improved; and moreover, all the components cooperate to regulate the growth of the crops and improve the stress resistance, and the surfactant also improves the utilization rate of the fertilizer and reduces waste.

The invention relates to the technical field of daily chemical and skin external preparations, in particular to a six-dimensional synergistic effect-based acne removal and repair composition, which is prepared from the following active ingredients in parts by weight: 1 to 10 parts of sturgeon caviar extract, 0.1 to 10 parts of chlorogenic acid, 0.1 to 20 parts of 4-tert-butyl cyclohexanol and 0.1 to 20 parts of willow extract. The sturgeon caviar extract is prepared by supercritical CO2 fluid extraction, the willow extract is treated by a synthase process, the content of salicin is not less than 1.5 wt%, and at least one active component in the composition is subjected to supramolecular inclusion modification; the preparation method comprises the following steps: sequentially preparing the extracts and components, sequentially mixing, and finally carrying out an inclusion reaction. The composition constructs a full-link acne removal and skin health maintenance system through a six-dimensional synergistic effect of bacteriostasis, oil control, anti-inflammation, allergy relief, cutin dredging and repairing, has the characteristics of efficient acne removal, mildness, safety and low recurrence rate, is suitable for various skin types such as sensitive acne skin, and can be widely applied to acne removal products of various dosage forms.

The invention relates to the technical field of collagen, in particular to a pig collagen chemical decellularization method based on a supercritical fluid technology, which comprises the following steps: taking fresh pigskin collagen, cleaning, cutting, and carrying out physical pretreatment; putting the pretreated collagen into a supercritical reaction kettle, introducing supercritical fluid, controlling the pressure to be 30-42 MPa and the temperature to be 40-50 DEG C, and maintaining for 10-20 minutes to establish a supercritical environment; injecting a mixed reagent containing ethanol, protease and a chelating agent into the supercritical reaction kettle, and stirring for 1.0-2.0 hours; after the treatment is finished, reducing the pressure of the reaction kettle in a stepped manner at the rate of 0.5-1MPa / min, introducing supercritical fluid or a mixed fluid of the supercritical fluid and ethanol for replacement during the period, and finally reducing the pressure to normal pressure; and taking out the collagen, carrying out enzymolysis treatment, and washing with a sterile solution to obtain the collagen. And the DNA residual quantity is greatly reduced through supercritical multiphase fluid cooperation and a nano-enzyme directional delivery technology.

The invention relates to the technical field of preparation of fermented drinks, in particular to a probiotic-enzymolysis synergistic fermentation functional tea beverage and a preparation method thereof. Aiming at the technical problems that the content of polyphenols in tea leaves is high, the growth of probiotics is inhibited, the use of mixed enzymes lacks time sequence control, and the activity of the probiotics is reduced in the shelf life, the invention provides the following scheme: carrying out directional enzymolysis pretreatment on tea raw materials, adopting response type enzyme-controlled release capsules to assist fermentation, and constructing a three-stage fermentation control strategy; tea soup before fermentation is pretreated through a pulsed electric field, initial infectious microbe load is reduced, and low-temperature sterilization is achieved; after fermentation, the probiotics are microencapsulated, so that the survival rate of the probiotics stored at normal temperature is remarkably increased. The functional tea beverage prepared by the invention is high in viable count of probiotics, good in stability, rich and harmonious in flavor and remarkable in nutritional and health-care effects.

The invention discloses a composition for growing and nourishing hair as well as a preparation method and application thereof. The composition is prepared from various components including 20 to 30 parts of cacumen biotae extract, 2 to 7 parts of eclipta prostrata extract, 3 to 9 parts of specnuezhenide and the like. The preparation method comprises the steps of raw material pretreatment, active ingredient extraction, compound blending, microcapsule embedding and preparation forming, the cacumen biotae is subjected to a supercritical extraction process, and the eclipta prostrata is subjected to an ultrasonic-assisted enzymolysis-subcritical water extraction combined process. Experiments show that the composition can significantly increase the hair growth rate, maintain the hair follicle growth period, increase the diameter of a hair bulb part, effectively improve hair follicle health through the synergistic effects of activating hair follicle stem cells, inhibiting apoptosis and the like, and can be applied to preparation of hair care products for promoting hair growth.

The invention relates to the technical field of separation and extraction, and provides an extraction method and application of chlorogenic acid in honeysuckle. The invention relates to a method for extracting chlorogenic acid from honeysuckle, which comprises the following steps: mixing honeysuckle powder with an ethanol solution, and carrying out ultrasonic enzymolysis; carrying out suction filtration to obtain a supernatant A containing chlorogenic acid and medicine residues I, and adding an ethanol solution into the medicine residues I to carry out microwave extraction; performing suction filtration to obtain a filtrate B and medicine residues II, mixing the medicine residues II with a citric acid-sodium citrate buffer solution, adding a Fe3O4-coated GO-coated MOFs nano-catalyst, performing hydrothermal reaction, performing suction filtration to obtain hydrolysate, and combining the hydrolysate with the supernate A and the filtrate B to obtain a total extraction solution; concentrating the total extracting solution under reduced pressure, and recovering ethanol until the volume of the concentrated solution is about 1 / 6-1 / 8 of the original volume; and concentrating under reduced pressure to thick paste, and drying to obtain the chlorogenic acid. The extraction efficiency and yield of chlorogenic acid are improved, and the utilization rate of raw materials and the product purity are improved.

The invention relates to the field of biological product preparation, in particular to a preparation method of high-activity oyster-derived small molecule peptide. The method comprises the following steps: selecting fresh meat of pacific oysters or ostrea rivularis as a raw material, degreasing, adding water, crushing, filtering, sequentially carrying out compound protease and neutral protease hydrolysis, implanting a sensor and a probe in hydrolysis, monitoring in real time, and dynamically adjusting reaction conditions; adding a targeting microcapsule wrapping an ascorbic acid glutathione composite protective agent, and releasing the protective agent when a target peptide signal is detected; separating and concentrating through a gradient membrane, and purifying the target peptide by using a molecular imprinting chromatographic column; carrying out alkaline hydrolysis on enzymatic hydrolysis residues to prepare chitosan for cyclic utilization, carrying out enzymatic hydrolysis on residual residues to generate collagen peptide, treating and recycling wastewater, and recovering protease. According to the method, the technical effects that the high-activity and high-purity target peptide is prepared, the resource utilization rate is increased, the production cost is reduced, the target peptide has good oxidation resistance and biological activity, the intestinal enzyme degradation rate is reduced after glycosylation modification, and the blood half-life period is prolonged are achieved.

The utility model discloses an enzymatic hydrolysis device for fruit essence, and belongs to the technical field of fruit essence production, the enzymatic hydrolysis device comprises a reaction tank body, a feed liquid storage tank, a standard alkali liquid storage tank, a standard acid liquid storage tank and an enzyme liquid storage tank, the reaction tank body is composed of a shell and an inner cylinder, an interlayer is formed by a gap between the shell and the inner cylinder, and the inner cylinder is arranged in the shell; a feeding hole is formed in the top end of the interior of the inner cylinder, and the feed liquid storage tank is communicated with the feeding hole through a connecting pipe; the device is novel in design and ingenious, the hydrolysis efficiency and the mixing uniformity are improved by optimizing a stirring structure and a control mode, accurate control over the reaction temperature is achieved by adopting an accurate temperature control system and a multilayer temperature sensor layout, the product quality stability is improved, and by additionally arranging a high-definition camera and an automatic sampling device, the product quality stability is improved. The operation convenience and the remote monitoring capability are improved, the maintenance cost is reduced, the production automation level is improved, and the practicability of the device is greatly improved.

The invention relates to a gastric acid-resistant sustained-release intestinal bacteria capsule based on bionic mucous membrane-enzymolysis response and a preparation method thereof. The gastric acid-resistant sustained-release intestinal bacteria capsule comprises a core layer, an adhesive layer, an outer-layer composite film and a protective coating from inside to outside, the core layer consists of intestinal flora and a multifunctional freeze-drying protective agent; the adhesive layer is composed of trehalose, N-acetyl glucosamine and MUC2 recombinant protein; the outer-layer composite film is composed of a base material and a functional filler; the protective coating is composed of beewax and chitosan. The anti-gastric acid sustained-release intestinal bacteria capsule based on bionic mucosa-enzymolysis response provided by the invention has excellent gastric acid resistance, and the gastric acid resistance is remarkably improved; good slow release performance is achieved, and accurate slow release of enzymatic hydrolysis response can be achieved; the colonization efficiency of the intestinal bacteria can be effectively improved; the storage stability is good. The preparation method provided by the invention has the advantages of simplified steps, short time consumption, controllable core operation time and obviously shortened whole process, and is beneficial to keeping the activity of the flora.

The invention belongs to the technical field of plant fermented beverages, and particularly relates to a synergistically fermented composite plant beverage and a preparation method thereof. The preparation method comprises the following steps: sieving platycodon grandiflorum and liquorice, and then carrying out ultrasonic pretreatment and cold extraction to obtain a platycodon grandiflorum and liquorice cold extraction solution; adding pectinase and beta-glucosidase into the pear juice, and carrying out enzymolysis composite treatment, so as to obtain pear juice after enzymolysis; mixing the platycodon grandiflorum and liquorice cold extraction liquid and the pear juice subjected to enzymolysis to obtain a cold extraction mixed liquid, and then inoculating a combined strain for fermentation to obtain a fermented mixed liquid; and performing molecular distillation and concentration on the fermentation mixed solution, and then performing high-pressure carbon dioxide sterilization and photodynamic sterilization in sequence to obtain the product. According to the preparation method disclosed by the invention, the preparation time is effectively shortened, the energy consumption is reduced, the preparation efficiency is improved, and the prepared composite plant beverage is high in content of active ingredients and nutrient elements such as GABA, platycodin and the like, long in storage time and high in storage stability.

The invention discloses a casein peptide composition containing multifunctional peptide and a preparation method of the casein peptide composition. The present invention provides a casein peptide composition containing a multifunctional peptide, characterized in that the casein peptide composition is obtained by enzymatic hydrolysis of a casein liquid, in the casein peptide composition, the relative strength of total functional peptides is 25% or more, the relative strength of casein phosphopeptides is 9% or more, and the relative strength of the casein phosphopeptides is 9% or more. The relative strength of the functional peptides except the casein phosphopeptides is above 11%, and the enzyme in the enzymolysis at least comprises serine protease.

The invention belongs to the field of biomass-based functional materials, relates to a difunctional coating based on enzymolysis modified cassava starch as well as a preparation method and application of the difunctional coating, and innovatively solves the technical problem that a single coating gives consideration to oil prevention and water absorption through a synergistic technical path of pretreatment and composite enzymolysis. The core mechanism is as follows: the starch particle structure is destroyed through pretreatment, and the degradation degree of starch is accurately controlled through the synergistic effect of alpha-amylase and saccharifying enzyme, so that an unexpected technical effect is achieved, and an enzymolysis product with a film-forming skeleton (medium molecular weight dextrin) and a hydrophilic unit (small molecular saccharide) is formed; meanwhile, a compact and continuous network structure can be formed by a film-forming framework, and grease permeation is blocked; and the hydrophilic unit endows the coating with a rapid capillary water absorption effect.

The present application relates to a kind of sensitive skin care composition with soothing, repair effect and its application, it is composed of 4-tert-butyl cyclohexanol, schisandra chinensis extract and grey hair bean seed extract, the mass ratio of 4-tert-butyl cyclohexanol, schisandra chinensis extract and grey hair bean seed extract is 1:(0.01-0.1):(0.01-0.1);Wherein, schisandra chinensis extract and grey hair bean seed extract are obtained using enzymatic hydrolysis-fermentation process, the composition of the present application has good soothing repair effect, and the active ingredient combination shows good compatibility and stability in formula system, without additional solubilizer and emulsifier, adapt more mild dosage form, more fit the needs of moderate sensitive skin.

The invention discloses an easily absorbable organic calcium powder containing mineral elements and a preparation process thereof, and the raw materials comprise, by weight, 50-70 parts of an organic calcium source, 20-30 parts of a plant protein source, 5-10 parts of a polybasic amino acid chelate, 0.01-0.03 part of vitamin D3, 1-3 parts of trace mineral elements, 0.5-1.5 parts of compound protease, 0.1-0.3 part of a food-grade surfactant, and 100-150 parts of deionized water. The invention relates to the technical field of nutritional supplements. According to the mineral element-containing easy-to-absorb organic calcium powder and the preparation process, vegetable protein is converted into amino acid through biological enzymolysis, the amino acid and calcium carbonate are subjected to a chelation reaction to generate amino acid chelated calcium, and compared with traditional organic calcium, chelated calcium is more easily absorbed by human intestinal tracts; meanwhile, calcium, magnesium and phosphorus are synergistically matched, magnesium can activate vitamin D3, the vitamin D3 further promotes absorption of calcium, phosphorus and calcium form a reasonable proportion, deposition of calcium in bones is enhanced, and calcium ions can be absorbed in intestinal tracts without depending on gastric acid by adopting a bicyclic stable chelating structure.

The application belongs to the technical field of polymer material recycling, and particularly relates to a high-efficiency enzymatic hydrolysis process of polycaprolactam. The high-efficiency enzymatic hydrolysis process of polycaprolactam provided by the application comprises the steps of acid pretreatment, enzymatic catalytic depolymerization and product post-treatment. The process combines sulfuric acid and a multi-enzyme system to treat PA6 with high substrate concentration. The physical form and chemical accessibility of PA6 are significantly changed through sulfuric acid pretreatment, which creates conditions for subsequent enzymatic hydrolysis reaction, and realizes efficient, deep and green degradation of PA6.