2611 results about "Enzymatic hydrolysis" patented technology

The invention belongs to the technical field of soil fertilizers, and particularly relates to a soil conditioner and a preparation method thereof. Wherein the soil conditioner is prepared from the following raw materials in parts by weight: 30 to 40 parts of modified charcoal, 20 to 30 parts of microbial complexing agent, 15 to 25 parts of humic acid and 1 to 5 parts of biological enzyme; according to the microbial complexing agent, dopamine grafted and modified nano silicon dioxide and hydroxyapatite form a composite shell, pseudomonas aeruginosa and bacillus subtilis are wrapped, and the microbial complexing agent has the functions of protecting microorganisms, adsorbing heavy metals and slowly releasing phosphorus elements; the modified biochar is compounded with the attapulgite through acid-base treatment to form a porous structure with physical inlaying and chemical functional groups synergistic, so that the adsorption and fixation capability on heavy metals and organic pollutants is remarkably improved; the biological enzyme accelerates organic phosphorus conversion, and enzymatic hydrolysate provides a phosphorus source for microorganisms, promotes microorganism breeding, effectively increases the content of available phosphorus in soil, and enhances the stability of a remediation system.

The invention discloses a method for extracting extracellular vesicles from dried Sanguisorba officinalis rhizomes and application of the extracellular vesicles, and relates to the technical field of molecular biology and biomedicine.The method comprises the following steps that S1, rehydration is conducted, specifically, the dried Sanguisorba officinalis rhizomes are subjected to liquid nitrogen quick freezing and then ground into powder at the low temperature, the powder and hydration liquid are mixed according to the proportion of 1: 10 (w / v), the mixture is slowly stirred for 2-4 h at the temperature of 4 DEG C, and the extracellular vesicles are obtained; removing large-particle impurities by using sterile gauze or a filter screen, and collecting filtrate; according to the method, through the synergistic effect of multiple enzymolysis and in combination with cellulase, pectinase and the like, the degradation efficiency is remarkably improved, meanwhile, EDTA is chelated with metal ions, and the enzymolysis effect is enhanced. Short-time alternating ultraviolet light is introduced to activate'dormant 'vesicles, so that the yield and the biological activity are improved. Secondly, multiple protection mechanisms, such as vitamin C, EDTA (Ethylene Diamine Tetraacetic Acid), cane sugar, BSA (Bovine Serum Albumin) and an ultraviolet protective agent are adopted, so that the integrity and the biological activity of the vesicles are ensured.

The invention relates to the technical field of biological extraction, in particular to a green synergistic extraction system and an extraction method and application thereof.The green synergistic extraction system comprises a deep eutectic solvent system, an enzymolysis system and an ultrasonic-assisted extraction system, and the deep eutectic solvent system comprises a DES solvent; the DES solvent is composed of a hydrogen bond acceptor and a hydrogen bond donor, and the hydrogen bond acceptor is choline, choline chloride or L-proline; the hydrogen bond donor is lactic acid, urea or ethylene glycol; enzyme preparations in the enzymolysis system are at least two of cellulase, pectinase and beta-glucosidase; the problems that a traditional organic solvent is high in toxicity and poor in biodegradability and synthetic deep eutectic solvent raw materials are non-renewable are solved, a green synergistic extraction system which is environmentally friendly, low in cost and excellent in performance is developed, the mass transfer efficiency and the extraction yield are improved, the used DES and enzyme preparations can be recycled, and the cost is reduced while the environment is protected.

The invention discloses a clean label type crispness increasing method for small crispy meat, and belongs to the field of fried products. The preparation method comprises the following steps: firstly, efficiently preparing a lentinus edodes stem nanocellulose-soybean protein isolate compound by combining enzymolysis with a high-pressure homogenization method, preparing a gel-like emulsion with 50-70% of an oil phase by taking the lentinus edodes stem nanocellulose-soybean protein isolate compound as a dispersion phase, and then adding the gel-like emulsion as a pore-forming agent into paste, so as to develop a new way for increasing the crispness of the fried small crisp meat. The lentinus edodes stem nanocellulose-soybean protein isolate gel emulsion is applied to the small crispy meat, the crispness of the small crispy meat can be increased, the oil content and the oil oxidation degree of the small crispy meat can be reduced, the color and luster of the small crispy meat can be effectively regulated and controlled, and technical innovation and quality improvement in the field of fried foods are expected to be promoted.

The invention discloses an efficient denitrification method and system for landfill leachate, and the method comprises the following steps: diluting waste molasses, and then adding a compound enzyme preparation containing cellulase, pectinase and alpha-amylase for enzymolysis pretreatment to obtain a pretreatment solution; inoculating the pretreatment liquid with a compound flora containing clostridium butyricum and succinic acid-producing anaerobic spirillum, carrying out anaerobic fermentation, and separating to obtain a liquid carbon source containing acetic acid and propionic acid and fermentation residues; mixing the fermentation residues with waste sugar residues, and performing pyrolysis to prepare biochar; and synchronously adding the liquid carbon source and the biochar into a denitrification reactor, and carrying out denitrification on the landfill leachate. The total nitrogen removal load of the landfill leachate denitrification system is increased by 60% compared with that of a single carbon source process, and the sugar manufacturing solid waste recycling rate gt is achieved; 95%. According to the method disclosed by the invention, the dual goals of efficient denitrification and solid waste recycling are achieved through cooperative treatment of the waste molasses and the waste sugar residues in the sugar manufacturing process.

The invention discloses preparation of a whey protein peptide mixture with immune and muscle-building effects, and relates to the technical field of food processing and bioengineering. The neutral protease is adopted and the pH is adjusted, so that the enzymolysis efficiency is effectively improved, and the enzymolysis time is greatly shortened; meanwhile, the whey protein peptide which is uniform in molecular weight distribution, free of peculiar smell and good in thermal stability is successfully prepared by accurately controlling enzymolysis conditions and combining microbial fermentation. The protein peptide can effectively improve thymus indexes and spleen indexes, enhance the proliferation capacity of splenic lymphocytes and the activity of NK cells, play an excellent immune regulation role and have a remarkable muscle building effect. The preparation process has the advantages of high efficiency, low cost, simplicity, convenience and the like, gives consideration to high quality and high functionality of the product, and has great application value.

The invention discloses a salty taste enhancing peptide. The amino acid sequence of the salty taste enhancing peptide is YD, VR, DA, RVR or any one of SEQ ID NO: 1 to SEQ ID NO: 12. The salty taste enhancing peptide is derived from the part with the molecular weight smaller than or equal to 3000Da in substances obtained after enzymolysis of soft-shelled turtle eggs, has excellent binding energy with salty taste receptors ENaC receptor, TRPV1 receptor and TMC4 receptor and can be tightly combined with the salty taste receptors, a salty taste enhancing peptide solution with the concentration of 0.1% (g / mL) has a remarkable salty taste enhancing effect on a NaCl solution, salty taste sense can be effectively adjusted, and the salty taste enhancing peptide can be used for improving the salty taste of the soft-shelled turtle eggs. Therefore, the salt intake is reduced, the salty taste is not reduced, and the purposes of reducing salt and not reducing salty taste are achieved.

The invention provides a preparation method and application of a camellia polypeptide composition with anti-wrinkle, repairing and brightening effects. The camellia polypeptide composition comprises a component A and a component B, wherein the component A comprises a wrapping solubilizer and polyhydric alcohol; component B: a plurality of polypeptides; the component C comprises carnosine, camellia polypeptide and water. The camellia polypeptide disclosed by the invention is prepared by the following steps: performing supercritical deoiling on camellia seeds, performing alkali extraction, acid precipitation and enzymolysis to prepare polypeptide, performing membrane filtration, taking micromolecule polypeptide as a main component, promoting synthesis of type I, type III and type V collagen, promoting cell migration and scavenging free radicals, and has the effects of resisting wrinkles, repairing and resisting oxidation; according to the invention, the problem of water solubility of palmitoyl peptide and acetyl peptide is solved, so that palmitoyl peptide and acetyl peptide can form a stable composition together with water-soluble polypeptide. The anti-wrinkle, repairing and whitening effects are achieved in multiple ways and multiple directions, the effects of improving wrinkles, repairing the skin, brightening the skin color and delaying senescence can be better achieved, and the anti-wrinkle, repairing and whitening composition can be widely applied to various types of cosmetics.

The invention discloses a method for producing bacillus velezensis through solid-state fermentation of distiller's grains, and belongs to the technical field of fermentation engineering. The preparation method comprises the following steps: carrying out acid-base modification, enzymolysis and stacking fermentation treatment on white spirit vinasse, adding bran and jerusalem artichoke powder, carrying out steam explosion, and adding corn steep liquor dry powder, urea and other substances to prepare a white spirit vinasse culture medium; and carrying out amplification culture on the bacillus velezensis subjected to activation and seed culture by using a white spirit vinasse culture medium, drying and collecting the bacillus velezensis. Experimental results show that the bacillus velezensis can be efficiently produced by utilizing the fermentation method provided by the invention, and the spore number of the bacillus velezensis is as high as 9.2 * 10 < 10 > cfu / g. The technical method for producing bacillus velezensis through fermentation effectively solves the problem of resource utilization of white spirit vinasse and the problems of high cost and sewage in the liquid fermentation process, and is low in fermentation cost and suitable for industrial production.

The invention belongs to the technical field of biology, and relates to a combined method for improving the taste of cerasus humilis clear juice and increasing the content of available calcium and zinc, which comprises the following operation steps: S1, removing impurities from cerasus humilis fruits, cleaning, denucleating and crushing to obtain cerasus humilis pulp; s2, performing primary enzymolysis on the cerasus humilis fruit pulp to obtain primary enzymolysis cerasus humilis fruit pulp; s3, fermenting the cerasus humilis fruit pulp to obtain fermented cerasus humilis fruit pulp; s4, performing secondary enzymolysis on the fermented cerasus humilis fruit pulp to obtain secondary enzymolysis cerasus humilis fruit pulp; and S5, filtering, sterilizing and filling the secondary enzymolysis cerasus humilis pulp to obtain a cerasus humilis clear juice product. The combined method of high-speed dispersion machine release, pectinase enzymolysis, issatchenkia terricola fermentation and joint enzymolysis of cellulase, beta-glucosidase and alpha-rhamnosidase is utilized, the product taste is effectively improved while the yield of the cerasus humilis clear juice product is increased, and the cerasus humilis clear juice product is palatable in sourness and sweetness, clear, transparent and bright, full in flavor and suitable for large-scale production. And the contents of calcium and zinc are increased by more than 65% and 59%. The product has good calcium and zinc supplementing effects.

The invention relates to the technical field of biological medicine, and discloses a formula of compound probiotic enzyme powder for promoting gastrointestinal microecological balance and nutrient absorption, by constructing a cross feeding system of bifidobacteria, lactobacillus and butyric acid bacteria and combining a stomach-intestine stage enzyme activity gradient adaptation strategy and a microenvironment response type double-layer embedding technology, the gastrointestinal microecological balance and nutrient absorption can be promoted, and the gastrointestinal microecological balance and nutrient absorption can be promoted. And cross-scale synergy of flora metabolism, substrate conversion and targeted release is realized. Wherein the sodium alginate-pectin composite embedding structure forms a pH buffer micro-area in a gastric acid environment, and proton pump gene expression of the acid-resistant strain is synchronously triggered; the prebiotics compounding and step-by-step enzymolysis process breaks through a closed-loop path of fiber degradation-flora proliferation-short-chain fatty acid generation. Compared with a traditional single-strain intervention scheme, the method has the advantages that double limitations of flora function splitting and process isolation are broken through, and systematic technical advantages are formed in the aspects of improving flora colonization stability, enhancing metabolite conversion efficiency and inhibiting pathogenic bacterium colonization.

The invention relates to the technical field of functional beverage processing, in particular to a method for preparing ginger black tea by combining fresh ginger pretreatment with black tea probiotic fermentation, a ginger black tea product and application of the ginger black tea product. The method comprises the following steps: a) fresh ginger enzymolysis pretreatment: fresh ginger is subjected to enzymolysis treatment to reduce the gingerol content; b) microencapsulation of gingerol: preparing the ginger liquid subjected to enzymolysis, sodium alginate and chitosan into microcapsules; c) black tea extraction: performing water extraction on black tea to prepare black tea liquid; d) probiotic activation: carrying out activation culture on lactobacillus acidophilus and lactobacillus plantarum; e) performing multi-stage fermentation: mixing the black tea liquid, gingerol microcapsules and activated probiotics, and performing fermentation through multi-stage temperature and pH control; (f) post-processing the product, namely post-processing the fermentation liquor to obtain the probiotic-containing ginger black tea product, so that the effective components of the ginger are reserved while the content of spicy components of the ginger is reduced.

The invention relates to a medicine for repairing skin wounds and a preparation method thereof, and belongs to the technical field of biological medicines.The medicine is prepared from, by mass, 25-30 parts of bee embryo peptide, 8-10 parts of alkannin derivative, 10-15 parts of armillaria mellea polysaccharide, 8-10 parts of inonotus obliquus polysaccharide, 6-8 parts of notoginsenoside and 5-7 parts of astragaloside. The bee fetus peptide is a product which is more than 500Da and is obtained by performing step-by-step enzymolysis on bee larva freeze-dried powder through trypsin and bacillus subtilis neutral protease to obtain enzymatic hydrolysate which is less than 3kDa, performing purification through a G-50 sephadex column, performing ultrafiltration and performing freeze-drying. The alkannin derivative is obtained by reacting alkannin with succinic anhydride and glucosamine-polyethylene glycol. A special method is adopted for preparing the bee embryo peptide and alkannin derivatives, the bee embryo peptide and alkannin derivatives are matched with other components to play a synergistic role, and the effects of high safety, high skin cell proliferation promoting capacity, excellent antibacterial performance and the like are achieved.

The present invention discloses a natural composite flavor enhancer and a preparation method thereof, comprising the following steps: using an ultramicro grinder to crush rice wine lees and wheat gluten powder to obtain ultramicro wheat gluten powder and ultramicro rice wine lees powder; taking the ultramicro wheat gluten powder, sterile water, and protease, mixing them evenly, carrying out enzymatic hydrolysis, inactivating the enzyme after the reaction ends, filtering, and taking the filtrate, namely the wheat gluten powder enzymatic hydrolysate, for standby; taking the wheat gluten powder enzymatic hydrolysate, ultramicro rice wine lees powder, and sterile water, mixing them evenly, carrying out enzymatic hydrolysis, inactivating the enzyme after the reaction ends, filtering, and taking the filtrate, namely the composite enzymatic hydrolysate, for standby; taking the composite enzymatic hydrolysate, cysteine, xylose, glucose, granulated sugar, and salt, mixing them and carrying out a thermal reaction to obtain a reaction solution; and preparing the natural flavor enhancer by spray drying. The present invention utilizes the natural enzyme system in rice wine lees to synergistically enzymatically hydrolyze wheat gluten powder to promote the release of umami amino acids, and further strengthens the umami by using thermal reaction technology.

The invention belongs to the technical field of cosmetic preparation, and particularly relates to a skin moisturizing and repairing composition based on dendrobium officinale and fibroin as well as a preparation method and application of the skin moisturizing and repairing composition. Essence extracted from dendrobium officinale tender shoots obtained through a plant stem cell culture technology and stepped molecular weight fibroin prepared through a composite enzymolysis technology serve as raw materials, the safe and efficient composition is developed, and the actual requirements for skin moisturizing and repairing are met.

The invention relates to the technical field of collagen peptide extraction, and particularly discloses aquatic collagen tripeptide and a processing technology thereof, and the processing technology comprises the following steps: step S1, fish skin pretreatment; step S2, performing enzymolysis; step S3, fermentation; step S4, separation and purification; starting from three aspects, firstly, a modified degreasing agent is added in the degreasing treatment process, the modified degreasing agent takes 1-ethyl-3-methylimidazole acetate, zinc phytate and calcium citrate as main components according to a specific mass ratio, and secondly, gel emulsion is added in the enzymolysis process, so that the modified degreasing agent is prepared. The gel emulsion has an oil gel structure, grape seed oil and sodium lignin sulfonate, and 3, a composite strain is added in the fermentation process, the composite strain is prepared by mixing bacillus natto and lactobacillus plantarum according to a specific proportion, and the purity and the extraction rate of the aquatic collagen tripeptide are improved through the synergistic effect of all the procedures.

The invention belongs to the technical field of biological fermentation and cosmetics, and particularly relates to a cortex magnoliae officinalis fermented product with oil-controlling and dandruff-removing effects as well as preparation and application of the cortex magnoliae officinalis fermented product. The preparation method comprises the following steps: performing ethanol ultrasonic extraction on officinal magnolia bark powder to obtain an officinal magnolia bark extract; adding mangnolia officinalis, honeysuckle, green tea and pericarpium citri reticulatae powder into pure water, performing enzymolysis treatment by adopting cellulase and pectinase, then adding a carbon source, prebiotics and inorganic salt, performing high-temperature sterilization, performing cooling, then adding a mangnolia officinalis extract, and performing uniform mixing so as to obtain a fermentation culture medium; the method comprises the following steps: inoculating an aspergillus seed solution and a saccharomyces cerevisiae seed solution into a fermentation culture medium for first-step fermentation, heating and inactivating, cooling, inoculating fruit wine yeast for second-step fermentation, and sterilizing at high temperature, cooling and filtering after fermentation to obtain filtrate which is the cortex magnoliae officinalis fermented product with the effects of controlling oil and removing dandruff. According to the preparation method, enzymolysis and fermentation technologies are combined, and the honeysuckle, the green tea and the pericarpium citri reticulatae are fermented together, so that the antibacterial, anti-dandruff and oil-control effects of the mangnolia officinalis can be synergistically improved.

The invention relates to a method for separating micro-plastics from sediment rich in organic matters in mangrove forest, which comprises the following steps: sediment pretreatment: freeze-drying a sample, sieving, and oscillating and dispersing by using an alkaline buffer solution with the pH value of 9.0-10.0; performing density gradient centrifugation: sequentially performing step-by-step centrifugation by using a NaI solution with the concentration of 1.1-1.3 g / cm < 3 > and a ZnCl2 solution with the concentration of 1.5-1.7 g / cm < 3 >; enzymatic hydrolysis purification: digesting a centrifugal product with a compound enzyme solution at 35-40 DEG C for 4-8 hours; and vacuum filtration: collecting the micro-plastics by using a filter membrane with the aperture of 0.2-0.8 mu m. According to the method for separating the micro-plastics from the sediments rich in the organic matters in the mangrove forest, the density range of all common plastics is accurately covered by dual-density gradient centrifugation, the average recovery rate of 0.1-5mm micro-plastics in the sediments in the mangrove forest reaches 90.2 + / -3.5%, the degradation efficiency of typical organic matters in the mangrove forest by adopting organic matter interference elimination and cellulase and protease combination reaches 92.4%, and the degradation rate of the typical organic matters in the mangrove forest reaches 92.4%. Therefore, the separation efficiency is effectively improved, the contact ratio of particle size distribution curves of 1 mu m-5 mm before and after treatment reaches 98.2%, and the integrity of the micro-plastic can be effectively guaranteed.

The invention provides a fish collagen peptide with skin brightening and freckle removing effects as well as a preparation method and application thereof, and belongs to the technical field of protein peptides. The preparation method comprises the following steps: by taking fish skin and / or fish scales as raw materials, performing degreasing and fishy smell removal treatment, and then sequentially performing heat treatment, homogenization treatment, enzymolysis treatment and enzyme deactivation refining to obtain the fish collagen peptide. In the fish collagen peptide prepared by the invention, the proportion of polypeptide with the molecular weight of 189-3000Da is more than 90%, and the protein content is more than 99.23%. Experiments show that the fish collagen peptide can effectively reduce the skin color spot area proportion of a subject and improve the skin L value and ITA value of the subject at the same time. Therefore, the fish collagen peptide prepared by the invention has good skin brightening and freckle removing effects, and a new way is provided for preparing related products.

The invention provides an efficient enzymolysis fermentation method for hermetia illucens larvae. The efficient enzymolysis and fermentation method for the larvae of the hermetia illucens comprises the following steps: a, selecting fresh or chilled larvae of the hermetia illucens, firstly cleaning and removing impurities, then pulping by adopting hydraulic pressure, and controlling the particle size within the range of 1-5mm after pulping so as to ensure the subsequent enzymolysis and fermentation effects; b, according to the characteristics of hermetia illucens larvae, selecting compound protease and neutral protease as main enzymolysis agents, and mixing the raw materials, the enzymolysis agents and water according to the mass ratio of 10: 2: 5. According to the efficient enzymolysis and fermentation method for the hermetia illucens larvae, by optimizing the enzymolysis reaction and fermentation process, the antioxidant activity of polypeptide prepared from the hermetia illucens larvae can be effectively improved. By accurately selecting enzymolysis agents such as compound protease and neutral protease and reasonably combining fermentation strains, the method can significantly improve the antioxidant effect of the final product, so that the method has higher market competitiveness when being applied to the feed industry.

The invention discloses hawthorn leaf pectic polysaccharide with lipid-lowering activity and a preparation method and application thereof, and belongs to a preparation method of plant polysaccharide, the preparation method comprises the following steps: refluxing hawthorn leaves with ethanol, discarding monosaccharide and small molecule compounds, refluxing with hot water to extract medicinal materials, removing protein through an enzymolysis method and a Sevage reagent method, decolorizing with polyamide resin, and drying to obtain the hawthorn leaf pectic polysaccharide with lipid-lowering activity. The crude polysaccharide is separated through DEAE-52 column chromatography, and finally, the crude polysaccharide is further purified through Sephadex G-100, so that the uniform purified polysaccharide is obtained. Tests on pancreatic lipase inhibition, taurocholate binding capacity and glycocholate binding rate show that the compound has remarkable lipid-lowering activity and is expected to become a potential natural lipid-lowering preparation.

The invention belongs to the technical field of traditional Chinese medicines, and particularly relates to a composite sleep-aiding composition based on agilawood and a preparation method thereof. The preparation method comprises the following steps: carrying out ultrasonic-assisted enzymolysis on agilawood by adopting amylase and cellulase, carrying out mixed fermentation on the agilawood subjected to enzymolysis by inoculating lactobacillus plantarum, lactobacillus johnsonii and saccharomyces cerevisiae, and carrying out sterilization, filtration, concentration and drying treatment on the fermented agilawood to obtain the agilawood fermentation product. According to the method, the release rate of active substances in the agilawood is increased, the composite sleep-aiding composition containing the agilawood fermentation product has a remarkable sleep-aiding effect, the preparation method is simple, the problem that an existing agilawood product is poor in sleep treatment effect is solved, and the agilawood fermentation product is safe, free of irritation and high in application value.

The present invention provides an oil-controlling, anti-inflammatory, and soothing botanical composition, comprising the following raw materials in parts by weight: 3-5 parts of Centella asiatica extract, 1-2 parts of Licorice extract, 2-4 parts of Herba Cynanchifoliae extract, 1-4 parts of Epilobium angustifolium extract, 2-5 parts of Trifolium repens flower extract, 4-6 parts of Lemongrass leaf extract, 7-9 parts of Rosa multiflora root extract, and 0.1-0.5 parts of a vitamin complex. The present invention utilizes reflux extraction, enzymatic hydrolysis, and fermentation techniques to fully extract the active substances in the root. The resulting Rosa multiflora root extract exhibits synergistic effects with the other raw materials, providing excellent oil-controlling, anti-inflammatory, and soothing effects on the skin.

The invention discloses antioxidant salmon collagen peptide and a preparation method thereof, and belongs to the technical field of processing of salmon collagen peptide. The preparation method comprises the following steps: firstly, carrying out enzymolysis on the salmon material by using phospholipase A2, hydrolyzing abundant membrane phospholipids in the salmon material, destroying fat cell structures and releasing wrapped triglyceride, then carrying out secondary enzymolysis by using aspergillus oryzae lipase, hydrolyzing macromolecular lipids into free fatty acids which are easily dissolved in supercritical carbon dioxide, and carrying out secondary enzymolysis by using aspergillus oryzae lipase. The degreased salmon material is obtained by means of supercritical carbon dioxide extraction, the lipid removal rate is greatly increased, and the antioxidant salmon collagen peptide which is small in molecular weight and high in oxidation resistance is obtained through subsequent collagen extraction and enzymolysis.

The invention relates to a method for detecting activity of gamma-polyglutamic acid hydrolase and application. The method comprises the following steps: (1) preparing an enzymolysis reaction solution and a blank reaction solution; (2) preparing an NAC / OPA mixed solution; (3) adding an NAC / OPA mixed solution into the enzymatic hydrolysis reaction solution to obtain a reaction solution to be detected; (4) drawing a standard curve by taking L-glutamic acid as a standard substance; and (5) detecting the fluorescence intensity of the blank reaction solution and the reaction solution to be detected by using a microplate reader, and then calculating the activity of the gamma-polyglutamic acid hydrolase according to the fluorescence intensity and the standard curve. The method for detecting the activity of the gamma-polyglutamic acid hydrolase is high in detection speed, detection of a single sample can be completed within 25 min, and nearly real-time process monitoring is achieved. And the method has the advantages of reliable detection result and high accuracy, can be used for monitoring the enzyme activity of the gamma-polyglutamic acid hydrolase in the gamma-polyglutamic acid preparation process in real time and guiding the optimization of the fermentation process, and has extremely high industrial application value.

The present invention belongs to the field of deep processing of ginseng, and particularly relates to a ginseng extract that does not cause excessive internal heat and a preparation method thereof. The preparation method comprises the following steps: dicing and pulverizing dry ginseng to obtain ginseng powder; soaking the ginseng powder in water and naturally fermenting it, and then performing ultrasonic treatment to obtain an ultrasonic ginseng fermentation liquid; adding glycosidase complex enzyme to the ultrasonic ginseng fermentation liquid for enzymatic hydrolysis to obtain a ginseng enzymatic hydrolysis liquid; subjecting the ginseng enzymatic hydrolysis liquid to enzyme inactivation, centrifugation, and filtration in sequence to obtain a refined and purified ginseng liquid; and filling and sterilizing the refined and purified ginseng liquid in sequence. The preparation method of the present invention mainly performs deep "non-excessive internal heat" treatment on dry ginseng through processes such as pulverization, natural fermentation, ultrasonic treatment, enzymatic hydrolysis, centrifugation, and filtration. As can be seen from the detection results, the "excessive internal heat" components in the obtained ginseng extract stock solution have been greatly reduced, its scope of application has been greatly expanded, and it is more conducive to promotion.

The invention discloses a preparation method of a crocodile hydrolyzed protein zinc skin care product, and relates to the technical field of skin care. The method comprises the following steps: step a, pretreating raw materials: taking fresh crocodile meat and bones, cleaning, mechanically crushing until the particle size is less than 5mm, and adding water according to the ratio of 1: (3-5) to prepare homogenate; step b, performing three-step enzymolysis, namely performing enzymolysis by adopting alkaline protease, flavourzyme and zinc chelating enzyme in sequence; according to the constructed technology of three-step enzymolysis and three-stage membrane separation, accurate preparation of crocodile protein zinc is achieved, and the zinc chelating rate is increased to 90% or above through the gradient effect of alkaline protease, flavourzyme and zinc chelating enzyme and directional release of zinc binding sites and is remarkably higher than that of a traditional single-enzyme hydrolysis technology; meanwhile, a combined purification technology of a ceramic membrane, an ultrafiltration membrane and a nanofiltration membrane is adopted to replace chemical precipitation and organic solvent extraction, so that the completeness of active ingredients is reserved, the risk of environmental pollution is reduced, and the method conforms to the green manufacturing trend of food industry.

The invention belongs to the field of egg by-product processing, and particularly relates to a preparation method and application of eggshell membrane peptides, the preparation method of the eggshell membrane peptides comprises the following steps: S1, drying and crushing cleaned eggshells, and then separating eggshell membranes; s2, putting the eggshell membrane into a steam explosion machine, carrying out pressure maintaining treatment for 1-5 minutes under the pressure of 1.0-1.5 MPa, then instantly releasing the pressure, and collecting a steam explosion sample; s3, adding the eggshell membrane subjected to steam explosion treatment into a sodium hydroxide aqueous solution with the pH value of 7-9 according to the solid-liquid ratio of 1: (20-30), then adding alkaline protease according to the enzyme addition amount of 5000-15000U / g, carrying out enzymolysis for 5-10 hours at the temperature of 35-65 DEG C, then inactivating for 3-5 minutes at the temperature of 100 DEG C, filtering enzymatic hydrolysate, taking filtrate, centrifuging, taking supernate, and freeze-drying to obtain polypeptide; s4, polypeptide obtained in S3 is dissolved in an activation solution to be activated for 8-10 h at the temperature of 4-8 DEG C; and S5, separating the sample activated in the step S4 through a Sephadex G-15 chromatographic column to obtain the activated eggshell membrane peptide. Based on the method, the preparation efficiency is high, and the prepared eggshell membrane peptide is high in antioxidant activity.

The invention provides an extraction method of hirudin, an oral preparation containing the hirudin and a preparation method of the oral preparation, and belongs to the technical field of leech processing and traditional Chinese medicine. The hirudin extraction method comprises the following steps: S1, soaking fresh leeches in a freeze-drying protective agent, taking out the leeches, wiping the surfaces of the leeches, pre-freezing the leeches at the temperature of 40 DEG C below zero to 50 DEG C below zero for 1-2 hours, and freeze-drying the leeches at the temperature of 20 DEG C below zero to 30 DEG C below zero for 4-6 hours to obtain freeze-dried leeches; s2, crushing the freeze-dried leeches, adding the crushed leeches into water, adding enzyme and lecithin, performing enzymolysis for 4-6 hours under the conditions that the temperature is 35-40 DEG C and the pH value is 7.5-8.5, and cooling to room temperature to obtain an enzymolysis product; s3, sterilization, ultrafiltration and drying are conducted, and hirudin is obtained. The hirudin extracted by the method is high in activity and high in recovery rate.

The invention discloses a selenium-rich bimorphic actinidia arguta enzyme as well as a preparation method and application thereof, and belongs to the technical field of microbial fermentation. The preparation method of the selenium-rich bimorphic actinidia arguta enzyme comprises the following steps: (1) performing selenium-rich culture on saussurea involucrata to obtain selenium-rich saussurea involucrata; (2) pulping the whole actinidia arguta fruits, performing enzymolysis, inoculating the selenium-rich saussurea involucrata bacteria, and performing fermentation culture; and (3) after the fermentation culture is finished, carrying out solid-liquid separation to obtain the selenium-rich solid-state actinidia arguta enzyme and the selenium-rich liquid-state actinidia arguta enzyme. The selenium-rich saussurea involucrata is used for whole-fruit fermentation of actinidia arguta for the first time, a bimorphic enzyme product is developed, the selenium content in the actinidia arguta enzyme is increased, and the enzyme flavor and antioxidant activity are enhanced. Compared with the prior art, the method has originality in the aspects of fermentation strains, culture modes, product forms and biological functions, and a whole set of processing technology which is simple in process and high in operability is provided.