6031 results about "Fungus" patented technology

The invention discloses a capture probe group for detecting pathogenic microorganisms and application of the capture probe group. The nucleic acid sequence of the capture probe group comprises a sequence as shown in SEQ ID NO. 1 to SEQ ID NO. 101. The capture probe group can cover and detect 119 types of bacteria, 32 types of fungi, 41 types of DNA viruses and 71 types of RNA viruses and covers common infectious disease pathogens, the kit containing the capture probe can cover and detect 88 types of drug-resistant and virulence genes, the detection performance of pathogenic microorganisms is greatly improved, and the sensitivity and specificity are improved.

The invention belongs to the technical field of biochemical engineering and the technical field of bioengineering, and discloses a schizochytrium limacinum engineering strain for producing EPA (eicosapentaenoic acid) by prolonging a desaturation path. Part or all of the extended desaturase genes from thraustochytrium sp. And fusarium sp. And saprolegnia are expressed. According to the invention, extended desaturase genes from various sources are integrated into a schizochytrium limacinum genome, so that a schizochytrium limacinum gene engineering strain efficiently expresses the extended desaturase genes, and a heterologous EPA synthesis path with high synthesis efficiency, short culture period, simple purification method and low cost is provided; a basis is provided for producing grease rich in EPA from schizochytrium limacinum, and the problems of non-sustainability, high cost and the like caused by obtaining EPA from fishes are solved.

The invention discloses a coal-based solid waste ecological restoration material and a preparation method thereof. The preparation method comprises the following steps: mixing coal gangue powder with a cementing material, a mineral activator and a physical pore-forming foam body, and curing to prepare a porous sandy soil matrix; and uniformly mixing the prepared porous sandy soil matrix with coal slime and a complex microbial inoculant, and performing fermentation and precipitation to obtain the coal-based solid waste ecological restoration material. Due to the addition of the cementing material, the strength of a sandy soil matrix framework is enhanced, collapse of pore channels in the crushing process is reduced, and the water retention and air permeability of the material are improved. Compared with traditional aluminum powder chemical foaming and strong acid foaming, the physical foaming technology adopted by the invention improves the operation safety while reducing the cost, and facilitates standardized production. Nutrient elements in the ecological restoration material can be fully released through the activation effect of the bacterium-fungus complex microbial inoculant. The ecological restoration material can be applied to environmental restoration fields such as mine soil restoration and land reclamation.

The invention discloses an endophytic fungus LW111 and application thereof, and belongs to the technical field of microorganisms. The endophytic fungus LW111 is classified and named as Magnaporthaceae sp.LW111, and is preserved in the China Center for Type Culture Collection (CCTCC), and the preservation number is CCTCC NO: M 20241750. The endophytic fungus LW111 is co-cultured with rice, so that the endophytic fungus LW111 is colonized in the root tissue of the rice, the tolerance of rice plants to cadmium, especially in the seedling stage, can be enhanced, and the growth of the plants is promoted. Therefore, the endophytic fungus LW111 has popularization and application values in the aspects of guaranteeing plant health and improving rice seedling stage growth.

The invention relates to the technical field of agricultural micro-ecological application, and discloses a method for improving the growth and drought resistance of oats by using arbuscular mycorrhizal fungi, the arbuscular mycorrhizal fungi having a close symbiotic relationship with the oats are selectively utilized, and the symbiotic relationship between the arbuscular mycorrhizal fungi and the oats is established in a condition control mode. Oat growth is promoted, and drought adaptability is improved. The AM fungi used in the method are selected from rhizosporidium intraradices or glomus mosseae, corn or trifolium repens is taken as a host, the two AM fungi are propagated in a propagation medium respectively, then two arbuscular mycorrhizal fungi inoculants and oat are co-cultured in a symbiotic culture medium respectively, and the two arbuscular mycorrhizal fungi inoculants and oat are co-cultured in a symbiotic culture medium respectively. An effective way for improving beneficial symbiosis of AM fungi and hosts under adverse conditions is provided, the infection symbiosis relation of the AM fungi to host plants is improved manually, and the yield of dry land oats is increased.

The invention relates to the technical field of wood corrosion prevention and corrosion resistance treatment, in particular to a preparation process of a coating for improving the antibacterial and corrosion-resistant performance of wood. In order to improve the antibacterial and corrosion-resistant capabilities of the prepared coating, a corrosion-resistant antibacterial agent is prepared and added into the raw materials of the coating, and quaternary ammonium salt, guanidyl and other group structures are introduced into the corrosion-resistant antibacterial agent, so that the antibacterial performance on microorganisms such as fungi is realized; the introduction of fluorine can effectively improve the hydrophobicity of a coating of the paint and reduce the influence of water vapor on antibacterial components in the coating, and the introduction of allyl can enable the corrosion-resistant antibacterial agent to react with butyl acrylate and other raw materials under the action of an initiator to achieve the cross-linking ability, so that the loss of the corrosion-resistant antibacterial agent is effectively avoided, and the service life of the coating is prolonged. The antibacterial activity is kept for a long time.

The invention belongs to the technical field of soil improvement, and discloses a wormcast biochar-based soil conditioner as well as a preparation method and application thereof. The soil conditioner is prepared from the following raw materials: modified charcoal, wormcast, a seaweed fertilizer, a microbial agent, vermiculite, humic acid, urea and coal gangue. The modified biochar and the wormcast are used as main components, the modified biochar is loaded with potassium and phosphorus, and potassium and phosphorus elements can be slowly released and provided; on one hand, plasma modification can generate oxygen-containing groups and increase active sites, so that heavy metals are complexed, and the removal capacity of the heavy metals is improved; on the other hand, holes in the surface of the biochar can be increased, the specific surface area is increased, the adsorbability is improved, and the biochar can serve as a carrier of components such as microorganisms, wormcast and urea, so that nutrient substances are slowly released in soil. Wormcast contains some bacteria, fungi and the like, promotes plant growth and serves as organic fertilizer together with seaweed fertilizer and humic acid, and the content of organic matter and mineral matter in soil is increased.

The invention discloses a cladosporium sp. Fungus YS37 and application thereof in promoting rice growth, and belongs to the technical field of microorganisms. The cladosporioides sp. Fungus YS37 is a new species separated from the root system of wild rice, is named as Parasarocladium oryzae YS37, is preserved in the China Center for Type Culture Collection (CCTCC), and has a preservation number of CCTCC NO: M 20242286, and the cladosporioides sp. Fungus YS37 is a new species which is separated from the root system of wild rice and is named as Parasarocladium oryzae YS37. The Parasarocladium oryzae YS37 strain can be used for preparing a biological bacterial fertilizer, and when the Parasarocladium oryzae YS37 strain or a fermentation product of the Parasarocladium oryzae YS37 strain is applied to a rice root system, the growth of rice can be effectively promoted, and the plant biomass is increased. The invention provides a new way for application of microorganisms to development of crop production.

A system for acquiring data regarding a wound in tissue comprises at least one excitation light source configured to directly illuminate a wound with excitation light; a spectral filtering mechanism configured to permit passage of optical signals responsive to illumination of the wound and having at least one wavelength corresponding to bacterial, fungal, and / or other microorganism autofluorescence and / or bacterial, fungal, viral, and / or microbial fluorescence, the spectral filtering mechanism including a plurality of selectable filters respectively corresponding to different discrete spectral bandwidths; an optical sensor configured to detect the spectrally filtered signals; and a processor configured to receive the detected, filtered signals and to identify a fluorescent signature of bacteria in the wound based at least in part on the detected, filtered signals and to output data regarding the bacterial, fungal, viral, and / or microbial fluorescent signature.

The invention provides a degradable environment-friendly packaging material and a preparation process thereof, and belongs to the technical field of packaging materials. The preparation method comprises the following steps: preparing the modified nano montmorillonite; preparing a composite antibacterial additive; preparing a barrier additive; and preparing the degradable environment-friendly packaging material. The preparation method comprises the following steps: by taking methacrylic acid (2-dimethylaminoethyl) ester and acrylic acid as raw materials, modifying nano-montmorillonite under the initiation of benzoyl peroxide, performing quaternary ammonium salinization, and performing in-situ synthesis of nano-zinc oxide on the surface of the quaternary ammonium salinized montmorillonite through a hydrothermal reaction to prepare the composite antibacterial additive. When the composite antibacterial additive is added into polylactic acid to prepare a packaging material, the growth of various microorganisms such as bacteria and fungi can be effectively inhibited, the packaging material is endowed with strong antibacterial protection capability, and the composite antibacterial additive has a more lasting antibacterial effect so as to prolong the antibacterial validity period of the packaging material.

The invention belongs to the technical field of enzyme engineering and biology, and particularly relates to a fungal luciferase mutant and application of the fungal luciferase mutant in improving the bioluminescence intensity of fungi or plants, and the amino acid sequence of the fungal luciferase mutant is shown as SEQ ID NO.1. The fungal luciferase mutant NnLuzT138P is obtained by mutating fungal luciferase NnLuz, the NnLuzT138P and other FBP luminescence pathway genes are co-expressed in fungi or plants, the bioluminescence intensity of the fungi and the plants can be remarkably improved, and compared with the prior art, the light quantum intensity is improved by more than two times. The invention provides a feasible scheme for construction of enhanced sustainable luminous fungi and creation of luminous plants.

Provided herein are methods, compositions, and kits for determining the spatial location of target nucleic acids, including endogenous and exogenous target nucleic acids, in a biological sample using padlock probes and substrates with spatially barcoded capture probes. Also disclosed herein are methods for determining a presence and / or location of a microbe (e.g., archaea, fungi, bacteria) in a biological sample, e.g., by determining the presence and / or location of a microbial target nucleic acid in the biological sample.

The invention belongs to the technical field of microbiology and agricultural application thereof, and particularly relates to talaromyces sp., a microbial agent and application of the talaromyces sp. According to the invention, a fungus with antagonistic action on fusarium oxysporum is screened from the root of black nightshade, the fungus is identified as talaromyces, the inhibition rate of the strain on fusarium oxysporum is 68.4%, and pot experiment shows that the relative control effect of the strain on tobacco root rot is 61.1%; a field experiment result shows that the relative prevention effect of the strain on the tobacco root rot is 88.8%, the disease index of the tobacco root rot can be effectively reduced, large-area infection of pathogenic bacteria is inhibited to a certain extent, and meanwhile, the talaromyces sp. Strain screened by the invention can induce the activity of defense enzymes PPO, PAL and POD of tobacco seedlings, improve the disease resistance of the seedlings and protect the growth of tobacco plants. Therefore, talaromyces sp. Has great potential value in the aspect of preventing and treating tobacco root rot.

The invention provides a streptomyces, which is named as Streptomyces sp. And is registered and preserved in Guangdong Microbial Culture Collection Center, and the preservation number is GDMCC No: 65917. The invention also provides a fermentation broth of the streptomyces, and an ethanol extract and a crude extract of the fermentation broth. The novel streptomyces, the crude extract thereof and the like have broad-spectrum antibacterial activity, have a good antagonistic effect on various pathogenic bacteria such as banana long leaf spot germs and banana colletotrichum gloeosporioides, can enable hyphae of the banana colletotrichum gloeosporioides to shrink, deform and break, inhibit spore germination of the banana colletotrichum gloeosporioides, and have a good anti-bacterial effect on the banana colletotrichum gloeosporioides. According to the present invention, with the application of the bacterial strain, the colletotrichum gloeosporioides spores are shrunk and broken, the growth of the colletotrichum gloeosporioides is inhibited, the peroxidase activity, the polyperoxidase activity, the CAT activity and the like in the banana peel can be improved, and the bacterial strain is the potential biological agent for the prevention and control of the colletotrichum gloeosporioides diseases, and has wide development space and good development and application prospects.

The invention provides a Kitasatospora xanthophycea JY01 strain and application thereof, the strain is separated from intestinal tracts of spring silkworms, has good disease-resistant and growth-promoting effects, and is specifically expressed in that cellulase, beta-1, 3-glucanase, siderophore and auxin IAA can be secreted; pot experiments show that the biocontrol efficiency of the microbial inoculum to wheat stalk rot is 57.90%, the biocontrol efficiency of the microbial inoculum to corn stalk rot is 46.41%, and the plant height and fresh weight are both significantly increased. The microbial agent is expected to be applied to preparation of biological bacterial fertilizer for effectively preventing and controlling soil-borne fungal diseases.

The invention discloses an extraction method and application of fungus source exosome-like nano vesicles. The extraction method comprises the following steps: preparing a fungus filtrate by a hot water method, carrying out centrifugation and filtration purification on the fungus filtrate, and then carrying out ultrafiltration interception to obtain the fungus-derived exosome-like nano-vesicles. The extracted fungus-derived exosome-like nano-vesicles have a lipid bilayer structure, are uniform in particle size distribution, are rich in lipid, nucleic acid and protein, are non-toxic, and have a remarkable anti-inflammatory effect; the extraction method provided by the invention is suitable for extracting exosome-like nano vesicles in various fungal samples, and has high yield and high activity.

The invention belongs to the technical field of fungal gene engineering, and discloses a shiitake mushroom ABC transporter gene LeABC2 and application thereof in regulation and control of shiitake mushroom heat resistance. Whole genome association analysis is carried out based on heat resistance phenotype data of 133 shiitake mushroom strains, a candidate gene LeABC2 for regulating heat resistance is obtained, a LeABC2 gene RNA interference vector is constructed, the gene function is researched through an agrobacterium tumefaciens-mediated genetic transformation method, and the result shows that the LeABC2 gene negatively regulates the heat resistance of shiitake mushroom hyphae, the heat resistance of the hyphae can be improved by interfering the expression of the gene, and the gene has a good application prospect. A new solution thought is provided for breaking through the problem that the yield and quality are influenced by high temperature in the mushroom industry.

According to the method disclosed by the invention, arbuscular mycorrhizal fungi and biochar are added while multiple-leaf goldenrain trees are planted in cadmium-polluted soil. Compared with a traditional remediation mode of independently utilizing plants or microorganisms or biochar, the microorganism-plant-biochar combined remediation has the advantages that the plants, the microorganisms and the biochar are co-cultured to form a symbiotic system, so that the cadmium content in the soil is remarkably reduced, the remediation capacity of the plants is enhanced, and meanwhile, the growth of the plants is remarkably improved. The strategy breaks through the limitation that in the prior art, only improvement of plant resistance is concerned, and the strategy more focuses on the double effects of improving the phytoremediation capacity and promoting the growth effect, so that the maximization of the phytoremediation effect is achieved.

The invention relates to a universal nano biological vesicle preparation method. The preparation method comprises the following steps: carrying out crude extraction on cells from bacteria, fungi, plants or animals to obtain a crude extracting solution; the crude extracting solution is placed in a low-temperature and high-pressure homogenizer to be subjected to low-temperature and high-pressure homogenizing treatment, then the nano vesicles are obtained, the low-temperature and high-pressure homogenizer comprises a high-pressure cavity, a homogenizing valve, a working cavity, a conveying system and a temperature control system, a slit is formed in the homogenizing valve, and the crude extracting solution located in the high-pressure cavity enters the working cavity through the slit of the homogenizing valve, the pressure intensity of the high-pressure cavity is 300-3000 bar, and the working temperature of the high-pressure homogenizer is 0-10 DEG C. The activation energy is reduced by controlling high-pressure and low-temperature environments, so that the original cell membrane structure is broken, the cell wall structure is destroyed, and phospholipid bimolecules are driven to aggregate and self-assemble to form the vesicles. For biological membranes derived from bacteria, fungi, plants and animals, the vesicles can be prepared by the preparation method disclosed by the invention, and the method has universality.

The invention relates to the technical field of microorganisms, in particular to bacillus velezensis F0b and an application of the bacillus velezensis F0b. The bacillus velezensis F0b is preserved in the Guangdong Microbial Culture Collection Center on April 26, 2024, the preservation date of the bacillus velezensis F0b is April 26, 2024, and the preservation number of the bacillus velezensis F0b is GDMCC (China General Microbiological Culture Collection Center): 64566. The bacillus velezensis has prevention and / or control effects on pyricularia oryzae, cabbage sclerotinia sclerotiorum, tomato fusarium wilt, banana fusarium wilt, rice leaf spot, peronophythora litchii, soybean carbon rot, colletotrichum capsici and corn leaf curvularia, and can be used for preparing broad-spectrum microbial source bactericides. The fermentation liquor of the bacillus velezensis can generate amylase, carboxymethyl cellulose and protease, and the fermentation liquor of the bacillus velezensis has prevention and / or control effects on vegetable pathogenic fungal diseases, namely cabbage sclerotiniose or pepper anthracnose.

The invention discloses a rice OsTCP11 gene and application of the rice OsTCP11 gene in rice disease resistance, and the nucleotide sequence of the OsTCP11 gene is as shown in SEQ ID NO.1; the method is realized by knocking out a rice OsTCP11 gene; the knockout is carried out by utilizing a CRISPR / Cas9 gene editing technology; the gene OsTCP11 is knocked out through a CRISPR / Cas9 (clustered regularly interspaced short palindromic repeats / associated protein 9) technology, and a knockout mutant ostcp11 is obtained; it is found through rice blast resistance identification that compared with wild type rice, the mutant ostcp11 is more resistant to diseases and small in disease spot area, the number of pyricularia grisea carried by diseased leaves is small, and it is indicated that OsTCP11 negatively regulates the resistance of rice to rice blast. The bacterial leaf blight resistance identification finds that compared with wild rice, the mutant ostcp11 is more resistant to diseases and shorter in disease spots, which indicates that the OsTCP11 negatively regulates the resistance of the rice to the bacterial leaf blight. The method is beneficial to cultivation of rice blast and bacterial leaf disease resistant rice varieties, and provides a basis for later screening of high-resistance and broad-spectrum-resistance rice varieties.

The invention provides a culture medium synthesized by truffle mycorrhiza and application of the culture medium. Belongs to the technical field of plant and microorganism crossing. According to the method, mushroom dregs for planting edible mushrooms are used as raw materials, after aerobic fermentation, the mushroom dregs, vermiculite and perlite are evenly mixed according to a certain proportion, the pH is adjusted to 7.4-7.6, the culture medium synthesized by truffle mycorrhiza is prepared, after fermentation, the edible mushroom dregs have good water retention and air permeability and have nutrient substances needed by growth of aseptic seedlings, and the nutrient substances are rich in nutrient substances needed by growth of the aseptic seedlings. The culture medium is an ideal culture medium for mycorrhizal seedling culture; high-temperature sterilization treatment is carried out on the fungus sticks before spawn running in the early stage, other mycorrhizal fungi competing with truffles do not exist, and symbiotic culture of aseptic seedlings and truffle mycorrhiza can be effectively promoted. The invention further provides application of the culture medium in truffle mycorrhiza production and a specific application method, and technical support is provided for further development of truffle mycorrhiza production.

The invention provides Yunnan golden camellia root endophytic fungi and application thereof, and relates to the technical field of crop disease control. The Yunnan camellia nitidissima root endophytic fungus is Lecanicillium vase JHC098 and is preserved in the China Center for Type Culture Collection, the preservation number is CCTCC NO: M 2025749, and a liquid fermentation liquor crude extract of the Lecanicillium vase can be applied to prevention and treatment of crop diseases. The invention overcomes the defects in the prior art, and the crude extract of the liquid fermentation liquor of the endophytic fungus Lecanicillium viculare JHC098 at the root of Yunnan golden camellia can effectively inhibit the growth of pathogenic bacteria such as citrus canker and tobacco bacterial wilt, thereby achieving the purpose of certain biological control.

The invention discloses a method for restoring soil heavy metal pollution by coupling soybean urease with a charcoal cementing agent, and relates to the technical field of soil heavy metal pollution restoration, and the method comprises the following steps: dynamic microbiome regulation and control: introducing photosensitive protein into soil functional bacteria; the acyl homoserine lactone analogue is synthesized and applied to soil, and is competitively combined with a microbial quorum sensing receptor to block an inhibitor to secrete a signal channel; according to the method, microbial urease secretion is regulated and controlled through optogenetics, an inhibitor signal is blocked through quorum sensing interference, and a microbiome is precisely regulated and controlled; the pH-responsive nanocapsule releases an inhibitor in a targeted manner, and the magnetic-responsive nanochain dynamically captures interfering bacteria, so that the limitation of passive adsorption is broken through; the gene editing plant releases coumarin to inhibit fungal metabolism, and the engineering mycorrhizal fungi physically shields an inhibitor to construct cross-biosphere cooperative defense; the urease activity and the heavy metal removal rate are remarkably improved, meanwhile, the soil microbial diversity is maintained, and the soil specificity interference problem is solved.

The invention relates to the technical field of microorganisms, in particular to talaromyces purpurogenus, strain fermentation liquor of the talaromyces purpurogenus and application of the talaromyces purpurogenus to prevention and treatment of northern corn leaf blight. The invention provides biocontrol fungus fermentation liquor for preventing and treating northern corn leaf blight and application of the biocontrol fungus fermentation liquor. The preservation number of a strain of the biocontrol fungus fermentation liquor is CGMCC No.41911. The strain and the fermentation liquor thereof can completely inhibit various pathogenic bacteria, active substances in the fermentation liquor are relatively stable, the active validity period is long, the systemic property is relatively strong, the strain is not easy to degrade, high temperature resistance and microwave radiation resistance are realized, the antibacterial effect of chemical agents can be remarkably improved, and the effects of pesticide reduction and synergism are remarkably achieved when the strain and the fermentation liquor are mixed with chemical pesticides; therefore, the environmental safety problem and the drug resistance caused by chemical pesticides are obviously reduced.

The invention relates to the field of biological control, and discloses screening of entomogenous fungi with high pathogenicity to prodenia litura and a compound application of the entomogenous fungi and tetraniliprole. The beauveria bassiana strain with high toxicity to the prodenia litura is collected from a tobacco field in polder town in Jianghua County, Yangzhou City, Hunan Province. The invention further provides an efficient, pollution-free and residue-free insecticide combining the tolfenpyrad and the beauveria bassiana, and the insecticide has a synergistic effect. Research on the compounding effect of the tetratolfenpyrad and the beauveria bassiana shows that the insecticide can show a remarkable synergistic effect after being compounded, the prevention and treatment effect is improved, the use dosage of a single chemical pesticide can be obviously reduced, environmental pollution is reduced, and the problems that an existing pesticide is prone to generating resistance, not obvious in pesticide effect and the like are effectively solved.

The invention relates to the technical field of disease and insect pest image analysis, in particular to an AI image recognition and grading method for field crop leaf disease and insect pests, which comprises the following steps: under the irradiation of a field fixed light source, synchronously acquiring a plurality of polarized reflection images around crop leaves at preset angle intervals; extracting a pixel polarization degree matrix of a leaf area in each polarization reflection image; inputting the pixel polarization degree matrix into a polarization transmission model, outputting a cuticle anomaly coefficient graph, and marking an area exceeding a preset anomaly threshold in the cuticle anomaly coefficient graph as a highlight display area; matching an infection type template library according to a highlight display area distribution mode in the abnormal coefficient graph; and calculating an infection intensity value by combining the diffusion gradient of the highlight area, and outputting a pest grade. According to the method, the boundary of the optical mutation region of the focus region is depicted, so that the physical interpretation of disease detection is improved, and distinguishable feature spaces are provided for different infection mechanisms (such as fungal growth layers and insect pest piercing and sucking points).

The invention belongs to the field of microorganisms, and particularly relates to banana endophytic bacillus velezensis JDB15 and application thereof. The novel endophytic bacillus velezensis JDB15 is separated from banana plants, has the characteristics of efficient inhibition of Foc4, growth promotion of bananas and broad-spectrum antagonism of various pathogenic bacteria, can inhibit the morbidity of corn southern leaf blight, tomato fusarium wilt, tobacco gray mold and peach anthracnose, has the effects of inhibiting the morbidity of various pathogenic fungi, and has the advantages of high efficiency, low cost and the like. And a good broad-spectrum effect is achieved. Meanwhile, the strain can kill nematodes, has wide biocontrol bacterium activity, can be developed deeply subsequently, and provides a sustainable and green solution for prevention and treatment of banana wilt and other diseases and insect pests.

The invention discloses hydrogel loaded with antibacterial polypeptide as well as a preparation method and application of the hydrogel. The preparation method of the hydrogel comprises the steps that antibacterial polypeptide and polyethylene glycol dimethacrylate are subjected to a cross-linking reaction, the hydrogel can be obtained, and the antibacterial polypeptide is obtained through polymerization synthesis of a cationic CBL monomer compound and an HBL monomer compound; the hydrogel has remarkable sterilization and anti-biofilm effects, and in-vitro cytotoxicity shows that the antibacterial polypeptide hydrogel product has no inhibition effect on normal cell growth; when the hydrogel dressing is used as a hydrogel dressing and is combined with novobiocin for use, more than 99% of antibacterial effect is achieved in a diabetes wound infection model and a fungal psoriasis infection model; and when being used as a hydrogel preparation to be combined with fluconazole, the compound has the effect of inhibiting growth of more than 99% of fungi in an animal vaginitis model.

The invention discloses a compound microbial agent for nitrogen fixation, carbon sequestration, synergism and stress resistance and application, and belongs to the technical field of functional microbial preparations, the compound microbial agent provided by the invention is prepared by taking combined nitrogen-fixing bacteria and high-yield urease inhibitor bacteria as cores and compounding salt-resistant, biocontrol, growth-promoting and carbon sequestration bacteria. The compound microbial agent provided by the invention can efficiently wake up nitrogen-fixing flora of a root system, accelerate nitrogen conversion in the atmosphere and play a role in nitrogen fixation and synergism on one hand, and can increase the content of organic carbon in soil and improve the fertility of the soil on the other hand, so that the quality of agricultural products is improved, and the purposes of increasing yield and income are achieved. In addition, the compound microbial agent also has stress resistance and disease prevention functions, and can relieve abiotic stress caused by a saline-alkali soil environment, prevent plant diseases caused by bacteria or fungi, improve the survival rate of crop cutting seedlings and enhance the continuous cropping resistance of crops.