86 results about "Microbial diversity" patented technology

The invention discloses a rapid restoration method for degraded grassland based on a microbial agent. The microbial agent is formed by compounding 15-30 parts of a nitrogen-fixing microbial agent, 10-20 parts of a phosphate-solubilizing microbial agent, 8-15 parts of a plant growth-promoting microbial agent, 25-40 parts of a humic acid carrier, 3-8 parts of an embedding agent sodium alginate and 5-12 parts of a nutritional agent molasses. Wherein the nitrogen-fixing bacteria are azotobacter chroococcum and alfalfa rhizobium (the viable count is greater than or equal to 5 * 10 < 9 > CFU / g), the phosphate solubilizing bacteria are bacillus megatherium (the viable count is greater than or equal to 1 * 10 < 10 > CFU / g), and the growth-promoting bacteria are bacillus subtilis and pseudomonas fluorescens (the viable count is greater than or equal to 2 * 10 < 10 > CFU / g). The microbial inoculum is subjected to activation, carrier adsorption, sodium alginate embedding granulation and CaCl2 cross-linking processes to be prepared into slow-release particles of 1-3 mm. 200-250 kg of organic fertilizer is applied to each hectare in a mild area, 300-400 kg of organic fertilizer is applied to each hectare in a moderate and severe area, and leymus chinensis and wheatgrass are sown in a mixed mode. After 90 days of application, the vegetation coverage is improved by 40% or above, the soil organic matter is increased by 25-40%, the microbial diversity index is larger than or equal to 3.5, and the comprehensive remediation cost is reduced by 53%.

The invention relates to the technical field of bioinformatics and medical artificial intelligence data processing, in particular to a colorectal cancer risk assessment system based on intestinal microbiological diversity, which comprises a multi-modal time sequence data acquisition module, a multi-modal time sequence data acquisition module, a target object microbiome time sequence feature data acquisition module and a target object microbiome time sequence feature data acquisition module, obtaining current medical resource load state parameters; the compliance entropy calculation module is used for resolving longitudinal compliance entropy representing the data uploading continuity willingness of the target object; the artifact interference identification module is used for generating a physiological disturbance probability value and a denoised pathological evolution feature vector; the double-constraint risk assessment module is used for calculating an initial pathological risk index of the target object; the dynamic decision scheduling module is used for determining a final dynamic intervention strategy for the target object; according to the method, the false positive and false alarm rate is remarkably reduced, and the technical pain point that real pathological evolution and environmental interference are difficult to distinguish by a traditional method is solved.

The invention belongs to the technical field of agricultural soil conditioners, and particularly relates to a soil conditioner, a preparation method and application. The invention provides a soil conditioner. The soil conditioner comprises fermented sludge, modified bentonite and lignosulfonate, the mass ratio of the fermented sludge to the modified bentonite to the lignosulfonate is (6-8): (2-3): (1-3). When the soil conditioner is applied to saline alkali soil, the pH value of the soil can be reduced, the organic matter content of the soil can be increased, the microbial diversity index (Shannon index) of the soil can be increased, and heavy metal in the soil can be passivated; meanwhile, the soil conductivity value is reduced. Compared with a traditional lime improvement method, the soil conditioner can avoid the risk of secondary alkalization and is good in application effect.

The invention discloses a red soil dry land soil microbial diversity detection method based on multicolor developing test paper, and belongs to the technical field of soil environment microbial detection. According to the method, test paper loaded with different color developing agents is designed, a specific color developing reaction is triggered by combining microbial metabolic activity, and color developing characteristics are converted into parameters such as microbial diversity indexes and dominant species proportions by utilizing an image analysis model. The detection device comprises an image acquisition module and a data analysis module, and quantitative association of color development features and diversity is realized through a machine learning model. Compared with the prior art, the method has the advantages that the cost is extremely low, the detection time is shortened to be within 30 minutes, the method is suitable for field real-time monitoring of the red soil dry land, an efficient, low-cost and portable detection tool is provided for soil health management, and the method is particularly suitable for soil health assessment of resource-limited areas.

The invention discloses a composition for synergistically regulating intestinal flora and application thereof. The composition comprises the following components in parts by weight: 10-80 parts of a cordyceps militaris extract, 2-40 parts of quercetin, 5-50 parts of prebiotics and 5-80 parts of nucleotide. The compositions provided by the invention supplement each other, have a synergistic effect, are compounded according to a scientific proportion, can improve the diversity of intestinal microorganisms of mice and the abundance of species, and can significantly improve intestinal inflammation symptoms in a mouse colitis model.

The invention discloses application of thiamine in preparation of a product for treating subclinical ketosis of dairy cows, and belongs to the field of biological medicines. Tests prove that compared with dairy cows suffering from subclinical ketosis (SCK), by adding thiamine, the content of beta-hydroxybutyric acid (BHBA), the number of bacteria in milk and the number of somatic cells can be remarkably reduced, and the milk yield, the milk fat content, the acetic acid content and the A / P ratio are remarkably increased; the microbial diversity of rumen and excrement of the dairy cow can be adjusted; a metabonomics result shows that metabolite up-regulated after the thiamine is added mainly focuses on pyruvic acid metabolism, glycolysis or gluconeogenesis, tyrosine metabolism, glycerophospholipid metabolism and biosynthetic pathways of phenylalanine, tyrosine and tryptophan. The conclusion is that when thiamine is added into the SCK dairy cow, synthesis and catabolism of BHBA of an organism can be effectively regulated and controlled by adjusting the content of succinyl-coenzyme A, then subclinical ketosis is relieved, rumen fermentation is promoted, and therefore the milk yield and the milk quality are improved.

The invention discloses a microbial composition for preventing and treating vomitoxin as well as a preparation method and application of the microbial composition. The microbial composition for preventing and treating vomitoxin is mainly prepared by mixing a component I and a component II according to a mass ratio of (50.0-0.1): (50.0-99.9), the component I comprises trichoderma harzianum powder, trichoderma asperellum powder and penicillium bayi powder; and the component II comprises potassium phosphite, alkene potassium phosphate and potassium tripolyphosphate. The preparation method comprises the following steps: adding the component I and the component II into a stirrer, and stirring and mixing to obtain the coating. The invention also discloses an application of the microbial composition for preventing and treating vomitoxin. The microbial composition disclosed by the invention can generate metabolites with a biocontrol effect, has efficient inhibitory activity on fusarium, can reduce generation of vomitoxin, can effectively regulate diversity of soil microorganisms, realizes crop yield increase and soil improvement, and is green and environment-friendly. The method and application are simple, the cost is low, and the method is suitable for agricultural application.

The invention relates to the field of soil microorganism sampling, and discloses an alpine region soil microorganism diversity in-situ stratified sampler which comprises a fixed seat, a multi-layer drilling mechanism is arranged on the surface of the fixed seat, the multi-layer drilling mechanism comprises a motor, an output shaft of the motor is fixedly connected with a gear, and the output shaft of the motor is fixedly connected with the gear. The center of the fixing base penetrates through and is rotationally connected with a rotating disc, and the center of the top end of the rotating disc is fixedly connected with an electric hydraulic rod. According to the invention, the motor drives the gear to mesh with the tooth block to drive the turntable to rotate, and cooperates with the electric hydraulic rod to push the drilling rod to rotate and move downwards to break through the frozen soil hardness limit; the interior of the drilling rod is divided into independent cavities through multiple sets of inclined partition plates in the drilling rod, a rotating handle can be driven through bevel gears a and b to drive a fixing sleeve and a sliding auger rod to operate, meanwhile, a protruding rod of the drilling sleeve slides along an annular groove of a feeding port, the auger rod is pushed to extend, and soil with different depths is accurately conveyed to the corresponding partition cavities.

A kind of microplastic toxicity exposure risk evaluation method for fish with wild grass carp as sample, the present application relates to the field of microplastic toxicity exposure risk evaluation, specifically relates to a kind of microplastic toxicity exposure risk evaluation method for fish with wild grass carp as sample.The present application is to solve the problem that the existing evaluation method is single, cannot comprehensively and accurately evaluate the toxicity exposure risk of microplastic to fish.Method: I, set experimental group;II, input wild grass carp to experimental group, record body length and weight;III, feed cultivation, respectively add corresponding microplastic particles, the control group does not add microplastic particles;IV, combine the weight change of wild grass carp, body length change, feeding rate, mortality and intestinal microbial diversity index for calculation;The higher the value of comprehensive index R, the higher the toxicity risk of microplastic to fish is evaluated.The present application is used to evaluate the toxicity exposure risk of microplastic to fish with wild grass carp as sample.

The invention belongs to the technical field of tobacco cultivation, and provides a tobacco quality-improving and efficiency-improving fertilization combination, a cordate houttuynia relay intercropping mode and a cordate houttuynia relay intercropping method.The tobacco quality-improving and efficiency-improving fertilization combination comprises a tobacco special fertilizer, a cake fertilizer and a microbial agent which have a synergistic effect so as to improve the tobacco planting soil micro-ecological environment and improve the tobacco quality and economic benefits; the microbial agent has the functions of improving the diversity of rhizosphere soil microorganisms and optimizing the community structure; the tobacco planting soil micro-ecological environment can be comprehensively improved, the agronomic characters, appearance quality and economic benefits of tobacco leaves are remarkably improved, the fertilizer utilization rate is increased, beneficial microorganisms are enriched, and effective technical support is provided for green and sustainable development of the tobacco industry.

This application relates to the field of topsoil quality testing technology, specifically to a method, system, device, and equipment for testing topsoil quality in power transmission and transformation projects. The method includes: collecting topsoil data from mixed soil samples at different depths in various research plots and standard topsoil data from standard mixed soil samples; obtaining comprehensive nutrient indices and comprehensive microbial diversity indices at the same depth for each research plot, as well as the average coupling characteristic value of the research plot; determining nutrient remediation factors and microbial remediation factors for the research plots, and constructing topsoil remediation factors; and determining the topsoil quality testing results for the power transmission and transformation projects in the research plots based on all topsoil data, average coupling characteristic values, and topsoil remediation factors. This application can improve the quality of topsoil quality testing in power transmission and transformation projects.

The invention belongs to the field of microorganisms, and particularly discloses an efficient phosphate-solubilizing bacillus J2 strain and application thereof in coal gangue remediation. The efficient phosphate-solubilizing bacillus J2 strain is obtained through screening according to microbial diversity and driving factors of various groups of coal gangue, and it is identified that a plurality of growth-promoting and stress-resistance-related functional genes exist in a J2 strain genome through whole genome sequencing, strain physicochemical property analysis and potting experiments. And the J2 strain and the interaction plant system thereof are further applied to an ecological restoration experiment base of the Shaoguan river coal gangue mining area ruins, so that the regreening of the to-be-restored area is successfully realized.

The invention provides a method for evaluating the influence of fermentation beds of different pretreated corn straws on padding flora. According to the method, pretreated corn straw is divided into a control group Con, a hydro-thermal treatment group HT and a Fenton treatment group FeZ, the influence of fermentation conditions with the pretreated corn straw as padding on fermentation bed flora is researched, and an efficient bacterium source is provided for rapid degradation of feces in the subsequent goose breeding process. Experimental results show that although the diversity of the flora of the fermentation bed is not obviously changed by the corn straws subjected to different pre-treatments, the degradation efficiency of the fermentation bed is improved by the hydrothermal and Fenton pre-treatments through enrichment of functional bacteria.

The present invention relates to a microbial agent for preventing and treating angelica root knot nematode disease and application thereof. The microbial agent comprises the following components: Bacillus velezine with a preservation number of CCTCC No: M2022923 ( Bacillus velezensis ), Bacillus mycoides with a deposit number of CCTCC No: M2022924 ( Bacillus Paramycoid ), Toosendan extract, amino oligosaccharides, fulvic acid and trace element fertilizers. The microbial agent can significantly improve root vitality, root enzyme activity and plant enzyme activity and proline content in the process of Angelica sinensis planting, improve crop resistance to nematodes; significantly increase soil pH, trace elements, enzyme activity and microbial diversity index, significantly reduce soil phenolic acid content, improve soil environment; and significantly reduce southern root-knot nematodes ( Meloidogyne incognita ) The number of second-instar larvae and the disease index can be reduced, and the nematode reduction rate can be increased. It has a significant effect on the prevention and control of southern root-knot nematode disease of Angelica sinensis and has broad application prospects.

This invention provides a nitrogen and phosphorus wastewater treatment system and method. The system includes a pre-anoxic tank, an inclined plate sedimentation tank, an anaerobic tank, an anoxic tank, a homogenization reaction tank, a synergistic oxidation tank, and a storage tank. The storage tank is connected to the homogenization reaction tank via a material transport pipeline, and the homogenization reaction tank is equipped with a stirring group. The storage tank contains a carbon-based composite material with a surface loaded with manganese oxides and magnesium oxides. The carbon-based composite material is added to the homogenization reaction tank through the material transport pipeline, and stirring ensures uniform mixing of the material and the mixed liquid, achieving homogenization and adhesion, and enhancing the subsequent synergistic oxidation effect. The carbon-based composite material provides a reaction interface for microorganisms, organic matter, and sludge, increasing microbial diversity and enabling synergistic oxidation with microorganisms to improve the degradation efficiency of nitrogen and phosphorus pollutants. This invention has a simple structure, does not require continuous aerobic aeration, and has advantages such as small footprint, short hydraulic retention time, low operating energy consumption, continuous influent and effluent, and high nitrogen and phosphorus pollutant removal efficiency.

The invention belongs to the technical field of microorganisms, and relates to a microbial diversity-based evaluation method of a Maotai-flavor liquor spreading, airing and yeast mixing process and application of the microbial diversity-based evaluation method. According to the method disclosed by the invention, a relationship is established between stacking fermentation and a yeast spreading and mixing process at the front end of stacking fermentation, and fermented grain microorganisms in a stacking fermentation link are associated with fermented grain microorganisms after spreading and mixing yeast, so that the method for evaluating different yeast spreading and mixing processes based on microbial diversity is formed; and optimization of a yeast spreading, airing and mixing process in the actual production of the Maotai-flavor liquor is facilitated.

This invention provides the application of *Bifidobacterium breve* in the preparation of products for treating gastric ulcers and gastric inflammation caused by *Helicobacter pylori*, belonging to the field of microbial technology. The *Bifidobacterium breve* NKU BB1-2 of this invention, with accession number GDMCC NO: 65956, can antagonize *Helicobacter pylori* colonization and alleviate the aggravation of gastric ulcers caused by *Helicobacter pylori*. In vitro, *Bifidobacterium breve* NKU BB1-2 exhibits good antagonistic ability against *Helicobacter pylori*, significantly inhibiting its colonization and growth. Animal experiments show that *Bifidobacterium breve* NKU BB1-2 can alleviate the aggravation of gastric ulcers caused by *Helicobacter pylori* infection in vivo, manifested as reduced gastric mucosal damage, reduced gastric inflammation, and increased gastric pH. Furthermore, *Bifidobacterium breve* NKU BB1-2 can significantly enhance gastric microbial diversity, regulate and improve the gastric microbial structure, and assist in antagonizing and eradicating *Helicobacter pylori* infection.

The invention provides a sludge carbon-based bioelectrochemical constructed wetland for enhancing sewage denitrification. The sludge carbon-based bioelectrochemical constructed wetland comprises a wetland reactor and a circuit connecting device, the wetland reactor comprises a wetland pool as well as a water distribution layer, a lower filter material layer, an anode layer, an upper filter material layer and a cathode layer which are sequentially arranged in the wetland pool from bottom to top; the circuit connecting device comprises a power supply device and an adjustable resistor electrically connected with the power supply device; the adjustable resistor is respectively and electrically connected with the anode layer and the cathode layer through conductive wires; wherein the anode layer and the cathode layer are sludge biochar coated by a conductive metal net; the adjustable resistor is electrically connected with the conductive metal nets in the anode layer and the cathode layer through conductive wires respectively; the bioelectrochemistry-constructed wetland is coupled with the sludge biochar, and the biocompatibility and catalytic characteristics of the sludge biochar are utilized, so that the diversity and activity of microorganisms in an anode region are effectively improved, and the reduction effect of a cathode region on anions is promoted, thereby improving the removal effect on total nitrogen.

The invention discloses a method and system for predicting forest carbon sequestration potential based on a soil quality index, and the method comprises the steps: continuously checking mixed forest sample plot data based on forest resources, employing quantile age sequence analysis, and distinguishing a high-quality forest sample plot from a low-quality forest sample plot according to the preset average aboveground carbon sequestration rate of forest sample plots in a forest time sequence. Respectively collecting high-quality and low-quality forest soil samples in the high-quality forest sample plot and the low-quality forest sample plot; detecting chemical component data of the high-quality and low-quality forest soil samples, selecting a soil quality evaluation minimum data set according to the chemical component data and the microbial diversity indexes, establishing a soil quality index model, and calculating soil quality indexes of the high-quality and low-quality forest soil samples; and performing regression analysis on the soil quality index and the carbon sequestration potential, and predicting the carbon sequestration potential according to the soil quality index. According to the method, the influence of the soil quality on the forest carbon sequestration potential is determined, and the forest carbon sequestration capacity is rapidly evaluated.

The invention belongs to the technical field of soil improvement, and relates to an enhanced double-layer coated nano mineral soil conditioner and a preparation method thereof.The preparation method specifically comprises the steps that nano minerals and natural polymer materials are mixed in a reaction system according to the mass ratio, and a core-shell material is formed; and mixing and stirring the core-shell material and the polymer hydrogel, and drying to obtain the double-layer coated soil conditioner. According to the conditioner, through dual modification of natural polymers and hydrogel, agglomeration and inactivation of nano minerals are effectively prevented, and the dispersity and long-term stability of the conditioner in soil are improved. Meanwhile, the porous light structure can improve the granular structure, water binding capacity and porosity of soil, promote microbial diversity and achieve synergistic interaction of carbon sequestration and soil improvement, the raw materials are environmentally friendly, the preparation process is mild, universality is high, the slow release characteristic is achieved, the content of organic matter in soil and the crop yield can be remarkably increased, and the application prospect is wide. The method is suitable for agricultural soil improvement and carbon sequestration and sink increase.

The invention discloses a low-yield field soil ecological restoration effect evaluation system, and belongs to the technical field of agricultural informatization and ecological restoration. Relates to a multi-source data automatic acquisition, intelligent fusion analysis and dynamic visualization technology. According to the system, multi-dimensional indexes such as soil physicochemical properties, microbial diversity and crop growth states can be comprehensively obtained, comprehensive analysis is carried out based on big data and an artificial intelligence algorithm, and scientific and dynamic evaluation of the ecological restoration effect is achieved. By integrating a decision support function and a visual human-computer interaction interface, the system provides an accurate basis for optimization and management of low-yield field soil remediation measures, the scientificity and management efficiency of remediation effect judgment are improved, and agricultural sustainable development and farmland quality improvement are promoted.

The invention discloses a method for analyzing microbial diversity and organic acid difference of yellow water of different years, which comprises the following steps: S1, carrying out sample standardization treatment: taking a yellow water sample, adding a DNA (Deoxyribonucleic Acid) protective agent into one part of the sample as a microbial analysis sample, and storing at low temperature; the other part of the sample is filtered by an organic phase filter membrane to be used as an organic acid analysis sample and stored at low temperature; s2, microbial community analysis: performing DNA extraction, high-throughput sequencing and bioinformatics analysis on a microbial analysis sample to screen differential bacteria; s3, organic acid difference detection: acidifying the organic acid analysis sample, purifying the eluent by using an extraction column, and carrying out vacuum concentration and constant volume; analyzing and evaluating differences among groups, and screening different organic acids; and S4, multi-omics correlation verification: constructing an abundance-content matrix of the differential bacteria and the differential organic acids, calculating a Pearson correlation coefficient to determine significant correlation, annotating a metabolic pathway of the differential bacteria, and establishing a correlation model of the differential bacteria, the characteristic organic acids and the metabolic pathway.

The invention relates to a domestic waste incineration plant leachate treatment system and a use method thereof, and relates to the technical field of sewage and wastewater treatment.The domestic waste incineration plant leachate treatment system mainly comprises a pretreatment system, an anaerobic system, an MABR biochemical system, a deep treatment system and an auxiliary system which are sequentially arranged, the auxiliary system comprises a sludge system, a heat exchange system, a dosing system and an odor collection system. The pretreatment system is used for removing most suspended matters, the heating system is used for preserving heat, the UASB reactor is used for performing anaerobic digestion and efficiently degrading organic matters, the MABR biochemical system is used for synchronously performing nitrification and denitrification for nitrogen removal, the heat exchange system is used for controlling the water temperature of the MABR biochemical system to be stable, the tubular ultrafiltration system is used for realizing separation of sludge age and hydraulic retention time, and the MABR biochemical system is high in microbial diversity and low in sludge yield; further pollutant removal is carried out through deep treatment, and finally, stable effluent quality is ensured. The device can realize full-quantitative treatment of the leachate of the household garbage incineration plant, and has the advantages of small occupied area, low carbon emission, low operation cost and the like.

The invention relates to the technical field of agricultural ecology and resource environment, and discloses a planting and breeding cycle management method with synergism of reduced fertilization and soil remediation. The method comprises the following steps: deploying an in-situ soil sensing network integrated with a microbial respiratory activity electrochemical sensor, and obtaining soil physicochemical and biological activity data in real time; livestock and poultry waste resource product component online monitoring and crop multispectral nutrition inversion information are combined; a soil-crop-cultivation coupled dynamic state characterization model is constructed; and generating a cooperative regulation and control instruction of partition variable fertilization and directional field returning of the organic materials, and performing optimization execution through closed-loop feedback. Through multi-source data fusion and intelligent decision making, accurate reduction of the chemical fertilizer and synchronous improvement of the soil ecological function are achieved, nitrogen fertilizer application can be reduced on the premise of stable yield, and the diversity of soil organic matter and microorganisms is improved.

The present invention provides a method for planting crops on heavy metal-polluted land around a sulfide ore concentration area, which comprises the following steps: after leveling the heavy metal-polluted land, soil profile sampling and detection are carried out to determine the pollution depth; digging holes or trenches according to the crop planting requirements, and the crops include arbors or shrubs; mixing biochar with sandy soil and filling it into the holes or trenches; after a period of time, digging open the planting holes or trenches at the positions of the holes or trenches; laying waste wood, an intermediate layer, fertilizer and mixed soil at the bottom of the planting holes or trenches; planting crops, and then filling and fixing them with mixed soil, and thoroughly watering them with an amino acid water-soluble fertilizer and / or a mineral source humic acid; laying a weed-proof cloth, and planting heavy metal hyperaccumulator plants between the crop rows. The present invention can restore the vegetation of the heavy metal-polluted land around the sulfide ore concentration area, generate green value-added benefits, avoid a large amount of earthwork and soil erosion, improve the soil structure, fertility and microbial diversity, and increase the vegetation coverage rate.

The invention relates to a method for realizing two-way control of the particle size of aerobic granular sludge by coupling hydraulic selection and floc addition, which comprises the following steps: in the aerobic granular sludge culture process, forming hydraulic selection pressure by gradually shortening the precipitation time to promote sludge granulation, and monitoring the particle size change condition of a system in the granulation process; when the particle size of the particles is rapidly increased, taking floc sludge as additional sludge, regularly adding the floc sludge into the system according to a certain proportion, slowing down the granulation speed of the sludge, strengthening the diversity and mass transfer capacity of microorganisms in the system, and monitoring the particle size change condition during the floc adding period; analyzing a regulation and control mechanism of hydraulic selection and floc addition on microbial population sensing behaviors in the granulation process; the particle size of the aerobic granular sludge is subjected to two-way regulation and control by hydraulic selection and floc addition, so that the granulation process is maintained in a reasonable interval, the sacrifice of microbial diversity and particle mass transfer capacity in the granulation process is made up, and the system is more stable in microbial cooperation.

The invention relates to the field of food, in particular to pediococcus acidilactici and fermented food prepared from the pediococcus acidilactici. The invention provides pediococcus acidilactici, and the preservation number of the pediococcus acidilactici is CGMCC (China General Microbiological Culture Collection Center) No. 31573. The microbial diversity of the jackfruit during the storage period after picking is analyzed based on Illumina PE300 high-throughput sequencing, indigenous bacteria suitable for processing and fermentation of the jackfruit are obtained through separation and purification, gene information and function prediction information of the indigenous bacteria are mastered by adopting 16S rDNA sequencing identification and whole genome sequencing, and the jackfruit with good fermentation flavor and high fermentation efficiency is obtained by optimizing the fermentation process. The jack fruit fermented leisure food is simple in process.

The invention relates to a method for extracting and separating coastal mud flat environment DNA and application of the method in microbial diversity monitoring, and belongs to the technical field of environment monitoring and biodiversity. The method specifically comprises the steps that a coastal mud flat sediment sample and a coastal mud flat interstitial water sample are collected; extracting coastal intertidal zone sediment and coastal intertidal zone interstitial water environment DNA (deoxyribonucleic acid); carrying out PCR (Polymerase Chain Reaction) amplification and high-throughput sequencing on the extracted environment DNA to obtain a coastal mud flat microbial community structure result; and comprehensively calculating a biodiversity index according to a coastal mud flat microbial community structure result detection result to obtain coastal mud flat microbial diversity data. According to the method, the extraction efficiency of the environmental DNA is remarkably improved, the coastal mud flat microorganism investigation steps are optimized, the completeness of coastal mud flat microorganism investigation is improved, and a coastal mud flat microorganism diversity investigation result is more accurate.

The application provides a soil conditioner and a soil health improvement method, and steps include: mixing activated illite and dry cow dung, adding an activation solution to the mixture according to a solid-liquid ratio of 1:5-1:20, first ultrasonic oscillation, then mixing for secondary activation, then washing with a large amount of water until pH is constant, drying to obtain a dried mixture; compounding the dried mixture with calcium oxide to obtain the soil conditioner. The application uses natural minerals and waste biomass-based raw materials to develop a non-iron-based, green and sustainable remediation material, which can realize the synergistic stabilization of Cd and As composite pollution in a karst area in southwest China, and can also realize the improvement of soil health indexes such as microbial diversity, soil TOC, CEC, available P and available K; the application can not only solve the technical problem of difficult simultaneous stabilization of Cd and As, but also avoid the influence of the stabilized material on soil health.

The present invention relates to animal food, in particular pet food comprising a combination of spray-dried plasma and fructo-oligosaccharides. The invention further relates to the use of such compositions for improving gastro-intestinal digestion, gut microbial diversity and fermentation, nutrient absorption, and / or immunity in an animal.