41 results about "Microbial diversity" patented technology

The invention relates to the technical field of bioinformatics and medical artificial intelligence data processing, in particular to a colorectal cancer risk assessment system based on intestinal microbiological diversity, which comprises a multi-modal time sequence data acquisition module, a multi-modal time sequence data acquisition module, a target object microbiome time sequence feature data acquisition module and a target object microbiome time sequence feature data acquisition module, obtaining current medical resource load state parameters; the compliance entropy calculation module is used for resolving longitudinal compliance entropy representing the data uploading continuity willingness of the target object; the artifact interference identification module is used for generating a physiological disturbance probability value and a denoised pathological evolution feature vector; the double-constraint risk assessment module is used for calculating an initial pathological risk index of the target object; the dynamic decision scheduling module is used for determining a final dynamic intervention strategy for the target object; according to the method, the false positive and false alarm rate is remarkably reduced, and the technical pain point that real pathological evolution and environmental interference are difficult to distinguish by a traditional method is solved.

The invention discloses a red soil dry land soil microbial diversity detection method based on multicolor developing test paper, and belongs to the technical field of soil environment microbial detection. According to the method, test paper loaded with different color developing agents is designed, a specific color developing reaction is triggered by combining microbial metabolic activity, and color developing characteristics are converted into parameters such as microbial diversity indexes and dominant species proportions by utilizing an image analysis model. The detection device comprises an image acquisition module and a data analysis module, and quantitative association of color development features and diversity is realized through a machine learning model. Compared with the prior art, the method has the advantages that the cost is extremely low, the detection time is shortened to be within 30 minutes, the method is suitable for field real-time monitoring of the red soil dry land, an efficient, low-cost and portable detection tool is provided for soil health management, and the method is particularly suitable for soil health assessment of resource-limited areas.

The invention discloses a composition for synergistically regulating intestinal flora and application thereof. The composition comprises the following components in parts by weight: 10-80 parts of a cordyceps militaris extract, 2-40 parts of quercetin, 5-50 parts of prebiotics and 5-80 parts of nucleotide. The compositions provided by the invention supplement each other, have a synergistic effect, are compounded according to a scientific proportion, can improve the diversity of intestinal microorganisms of mice and the abundance of species, and can significantly improve intestinal inflammation symptoms in a mouse colitis model.

The invention discloses application of thiamine in preparation of a product for treating subclinical ketosis of dairy cows, and belongs to the field of biological medicines. Tests prove that compared with dairy cows suffering from subclinical ketosis (SCK), by adding thiamine, the content of beta-hydroxybutyric acid (BHBA), the number of bacteria in milk and the number of somatic cells can be remarkably reduced, and the milk yield, the milk fat content, the acetic acid content and the A / P ratio are remarkably increased; the microbial diversity of rumen and excrement of the dairy cow can be adjusted; a metabonomics result shows that metabolite up-regulated after the thiamine is added mainly focuses on pyruvic acid metabolism, glycolysis or gluconeogenesis, tyrosine metabolism, glycerophospholipid metabolism and biosynthetic pathways of phenylalanine, tyrosine and tryptophan. The conclusion is that when thiamine is added into the SCK dairy cow, synthesis and catabolism of BHBA of an organism can be effectively regulated and controlled by adjusting the content of succinyl-coenzyme A, then subclinical ketosis is relieved, rumen fermentation is promoted, and therefore the milk yield and the milk quality are improved.

The invention discloses a microbial composition for preventing and treating vomitoxin as well as a preparation method and application of the microbial composition. The microbial composition for preventing and treating vomitoxin is mainly prepared by mixing a component I and a component II according to a mass ratio of (50.0-0.1): (50.0-99.9), the component I comprises trichoderma harzianum powder, trichoderma asperellum powder and penicillium bayi powder; and the component II comprises potassium phosphite, alkene potassium phosphate and potassium tripolyphosphate. The preparation method comprises the following steps: adding the component I and the component II into a stirrer, and stirring and mixing to obtain the coating. The invention also discloses an application of the microbial composition for preventing and treating vomitoxin. The microbial composition disclosed by the invention can generate metabolites with a biocontrol effect, has efficient inhibitory activity on fusarium, can reduce generation of vomitoxin, can effectively regulate diversity of soil microorganisms, realizes crop yield increase and soil improvement, and is green and environment-friendly. The method and application are simple, the cost is low, and the method is suitable for agricultural application.

The invention relates to the field of soil microorganism sampling, and discloses an alpine region soil microorganism diversity in-situ stratified sampler which comprises a fixed seat, a multi-layer drilling mechanism is arranged on the surface of the fixed seat, the multi-layer drilling mechanism comprises a motor, an output shaft of the motor is fixedly connected with a gear, and the output shaft of the motor is fixedly connected with the gear. The center of the fixing base penetrates through and is rotationally connected with a rotating disc, and the center of the top end of the rotating disc is fixedly connected with an electric hydraulic rod. According to the invention, the motor drives the gear to mesh with the tooth block to drive the turntable to rotate, and cooperates with the electric hydraulic rod to push the drilling rod to rotate and move downwards to break through the frozen soil hardness limit; the interior of the drilling rod is divided into independent cavities through multiple sets of inclined partition plates in the drilling rod, a rotating handle can be driven through bevel gears a and b to drive a fixing sleeve and a sliding auger rod to operate, meanwhile, a protruding rod of the drilling sleeve slides along an annular groove of a feeding port, the auger rod is pushed to extend, and soil with different depths is accurately conveyed to the corresponding partition cavities.

A kind of microplastic toxicity exposure risk evaluation method for fish with wild grass carp as sample, the present application relates to the field of microplastic toxicity exposure risk evaluation, specifically relates to a kind of microplastic toxicity exposure risk evaluation method for fish with wild grass carp as sample.The present application is to solve the problem that the existing evaluation method is single, cannot comprehensively and accurately evaluate the toxicity exposure risk of microplastic to fish.Method: I, set experimental group;II, input wild grass carp to experimental group, record body length and weight;III, feed cultivation, respectively add corresponding microplastic particles, the control group does not add microplastic particles;IV, combine the weight change of wild grass carp, body length change, feeding rate, mortality and intestinal microbial diversity index for calculation;The higher the value of comprehensive index R, the higher the toxicity risk of microplastic to fish is evaluated.The present application is used to evaluate the toxicity exposure risk of microplastic to fish with wild grass carp as sample.

The invention belongs to the technical field of tobacco cultivation, and provides a tobacco quality-improving and efficiency-improving fertilization combination, a cordate houttuynia relay intercropping mode and a cordate houttuynia relay intercropping method.The tobacco quality-improving and efficiency-improving fertilization combination comprises a tobacco special fertilizer, a cake fertilizer and a microbial agent which have a synergistic effect so as to improve the tobacco planting soil micro-ecological environment and improve the tobacco quality and economic benefits; the microbial agent has the functions of improving the diversity of rhizosphere soil microorganisms and optimizing the community structure; the tobacco planting soil micro-ecological environment can be comprehensively improved, the agronomic characters, appearance quality and economic benefits of tobacco leaves are remarkably improved, the fertilizer utilization rate is increased, beneficial microorganisms are enriched, and effective technical support is provided for green and sustainable development of the tobacco industry.

This application relates to the field of topsoil quality testing technology, specifically to a method, system, device, and equipment for testing topsoil quality in power transmission and transformation projects. The method includes: collecting topsoil data from mixed soil samples at different depths in various research plots and standard topsoil data from standard mixed soil samples; obtaining comprehensive nutrient indices and comprehensive microbial diversity indices at the same depth for each research plot, as well as the average coupling characteristic value of the research plot; determining nutrient remediation factors and microbial remediation factors for the research plots, and constructing topsoil remediation factors; and determining the topsoil quality testing results for the power transmission and transformation projects in the research plots based on all topsoil data, average coupling characteristic values, and topsoil remediation factors. This application can improve the quality of topsoil quality testing in power transmission and transformation projects.

This invention provides a nitrogen and phosphorus wastewater treatment system and method. The system includes a pre-anoxic tank, an inclined plate sedimentation tank, an anaerobic tank, an anoxic tank, a homogenization reaction tank, a synergistic oxidation tank, and a storage tank. The storage tank is connected to the homogenization reaction tank via a material transport pipeline, and the homogenization reaction tank is equipped with a stirring group. The storage tank contains a carbon-based composite material with a surface loaded with manganese oxides and magnesium oxides. The carbon-based composite material is added to the homogenization reaction tank through the material transport pipeline, and stirring ensures uniform mixing of the material and the mixed liquid, achieving homogenization and adhesion, and enhancing the subsequent synergistic oxidation effect. The carbon-based composite material provides a reaction interface for microorganisms, organic matter, and sludge, increasing microbial diversity and enabling synergistic oxidation with microorganisms to improve the degradation efficiency of nitrogen and phosphorus pollutants. This invention has a simple structure, does not require continuous aerobic aeration, and has advantages such as small footprint, short hydraulic retention time, low operating energy consumption, continuous influent and effluent, and high nitrogen and phosphorus pollutant removal efficiency.

The invention belongs to the technical field of microorganisms, and relates to a microbial diversity-based evaluation method of a Maotai-flavor liquor spreading, airing and yeast mixing process and application of the microbial diversity-based evaluation method. According to the method disclosed by the invention, a relationship is established between stacking fermentation and a yeast spreading and mixing process at the front end of stacking fermentation, and fermented grain microorganisms in a stacking fermentation link are associated with fermented grain microorganisms after spreading and mixing yeast, so that the method for evaluating different yeast spreading and mixing processes based on microbial diversity is formed; and optimization of a yeast spreading, airing and mixing process in the actual production of the Maotai-flavor liquor is facilitated.

This invention provides the application of *Bifidobacterium breve* in the preparation of products for treating gastric ulcers and gastric inflammation caused by *Helicobacter pylori*, belonging to the field of microbial technology. The *Bifidobacterium breve* NKU BB1-2 of this invention, with accession number GDMCC NO: 65956, can antagonize *Helicobacter pylori* colonization and alleviate the aggravation of gastric ulcers caused by *Helicobacter pylori*. In vitro, *Bifidobacterium breve* NKU BB1-2 exhibits good antagonistic ability against *Helicobacter pylori*, significantly inhibiting its colonization and growth. Animal experiments show that *Bifidobacterium breve* NKU BB1-2 can alleviate the aggravation of gastric ulcers caused by *Helicobacter pylori* infection in vivo, manifested as reduced gastric mucosal damage, reduced gastric inflammation, and increased gastric pH. Furthermore, *Bifidobacterium breve* NKU BB1-2 can significantly enhance gastric microbial diversity, regulate and improve the gastric microbial structure, and assist in antagonizing and eradicating *Helicobacter pylori* infection.

The invention belongs to the technical field of soil improvement, and relates to an enhanced double-layer coated nano mineral soil conditioner and a preparation method thereof.The preparation method specifically comprises the steps that nano minerals and natural polymer materials are mixed in a reaction system according to the mass ratio, and a core-shell material is formed; and mixing and stirring the core-shell material and the polymer hydrogel, and drying to obtain the double-layer coated soil conditioner. According to the conditioner, through dual modification of natural polymers and hydrogel, agglomeration and inactivation of nano minerals are effectively prevented, and the dispersity and long-term stability of the conditioner in soil are improved. Meanwhile, the porous light structure can improve the granular structure, water binding capacity and porosity of soil, promote microbial diversity and achieve synergistic interaction of carbon sequestration and soil improvement, the raw materials are environmentally friendly, the preparation process is mild, universality is high, the slow release characteristic is achieved, the content of organic matter in soil and the crop yield can be remarkably increased, and the application prospect is wide. The method is suitable for agricultural soil improvement and carbon sequestration and sink increase.

The invention discloses a low-yield field soil ecological restoration effect evaluation system, and belongs to the technical field of agricultural informatization and ecological restoration. Relates to a multi-source data automatic acquisition, intelligent fusion analysis and dynamic visualization technology. According to the system, multi-dimensional indexes such as soil physicochemical properties, microbial diversity and crop growth states can be comprehensively obtained, comprehensive analysis is carried out based on big data and an artificial intelligence algorithm, and scientific and dynamic evaluation of the ecological restoration effect is achieved. By integrating a decision support function and a visual human-computer interaction interface, the system provides an accurate basis for optimization and management of low-yield field soil remediation measures, the scientificity and management efficiency of remediation effect judgment are improved, and agricultural sustainable development and farmland quality improvement are promoted.

The invention discloses a method for analyzing microbial diversity and organic acid difference of yellow water of different years, which comprises the following steps: S1, carrying out sample standardization treatment: taking a yellow water sample, adding a DNA (Deoxyribonucleic Acid) protective agent into one part of the sample as a microbial analysis sample, and storing at low temperature; the other part of the sample is filtered by an organic phase filter membrane to be used as an organic acid analysis sample and stored at low temperature; s2, microbial community analysis: performing DNA extraction, high-throughput sequencing and bioinformatics analysis on a microbial analysis sample to screen differential bacteria; s3, organic acid difference detection: acidifying the organic acid analysis sample, purifying the eluent by using an extraction column, and carrying out vacuum concentration and constant volume; analyzing and evaluating differences among groups, and screening different organic acids; and S4, multi-omics correlation verification: constructing an abundance-content matrix of the differential bacteria and the differential organic acids, calculating a Pearson correlation coefficient to determine significant correlation, annotating a metabolic pathway of the differential bacteria, and establishing a correlation model of the differential bacteria, the characteristic organic acids and the metabolic pathway.

The invention relates to a domestic waste incineration plant leachate treatment system and a use method thereof, and relates to the technical field of sewage and wastewater treatment.The domestic waste incineration plant leachate treatment system mainly comprises a pretreatment system, an anaerobic system, an MABR biochemical system, a deep treatment system and an auxiliary system which are sequentially arranged, the auxiliary system comprises a sludge system, a heat exchange system, a dosing system and an odor collection system. The pretreatment system is used for removing most suspended matters, the heating system is used for preserving heat, the UASB reactor is used for performing anaerobic digestion and efficiently degrading organic matters, the MABR biochemical system is used for synchronously performing nitrification and denitrification for nitrogen removal, the heat exchange system is used for controlling the water temperature of the MABR biochemical system to be stable, the tubular ultrafiltration system is used for realizing separation of sludge age and hydraulic retention time, and the MABR biochemical system is high in microbial diversity and low in sludge yield; further pollutant removal is carried out through deep treatment, and finally, stable effluent quality is ensured. The device can realize full-quantitative treatment of the leachate of the household garbage incineration plant, and has the advantages of small occupied area, low carbon emission, low operation cost and the like.

The invention relates to the technical field of agricultural ecology and resource environment, and discloses a planting and breeding cycle management method with synergism of reduced fertilization and soil remediation. The method comprises the following steps: deploying an in-situ soil sensing network integrated with a microbial respiratory activity electrochemical sensor, and obtaining soil physicochemical and biological activity data in real time; livestock and poultry waste resource product component online monitoring and crop multispectral nutrition inversion information are combined; a soil-crop-cultivation coupled dynamic state characterization model is constructed; and generating a cooperative regulation and control instruction of partition variable fertilization and directional field returning of the organic materials, and performing optimization execution through closed-loop feedback. Through multi-source data fusion and intelligent decision making, accurate reduction of the chemical fertilizer and synchronous improvement of the soil ecological function are achieved, nitrogen fertilizer application can be reduced on the premise of stable yield, and the diversity of soil organic matter and microorganisms is improved.

The invention relates to a method for realizing two-way control of the particle size of aerobic granular sludge by coupling hydraulic selection and floc addition, which comprises the following steps: in the aerobic granular sludge culture process, forming hydraulic selection pressure by gradually shortening the precipitation time to promote sludge granulation, and monitoring the particle size change condition of a system in the granulation process; when the particle size of the particles is rapidly increased, taking floc sludge as additional sludge, regularly adding the floc sludge into the system according to a certain proportion, slowing down the granulation speed of the sludge, strengthening the diversity and mass transfer capacity of microorganisms in the system, and monitoring the particle size change condition during the floc adding period; analyzing a regulation and control mechanism of hydraulic selection and floc addition on microbial population sensing behaviors in the granulation process; the particle size of the aerobic granular sludge is subjected to two-way regulation and control by hydraulic selection and floc addition, so that the granulation process is maintained in a reasonable interval, the sacrifice of microbial diversity and particle mass transfer capacity in the granulation process is made up, and the system is more stable in microbial cooperation.

The application provides a soil conditioner and a soil health improvement method, and steps include: mixing activated illite and dry cow dung, adding an activation solution to the mixture according to a solid-liquid ratio of 1:5-1:20, first ultrasonic oscillation, then mixing for secondary activation, then washing with a large amount of water until pH is constant, drying to obtain a dried mixture; compounding the dried mixture with calcium oxide to obtain the soil conditioner. The application uses natural minerals and waste biomass-based raw materials to develop a non-iron-based, green and sustainable remediation material, which can realize the synergistic stabilization of Cd and As composite pollution in a karst area in southwest China, and can also realize the improvement of soil health indexes such as microbial diversity, soil TOC, CEC, available P and available K; the application can not only solve the technical problem of difficult simultaneous stabilization of Cd and As, but also avoid the influence of the stabilized material on soil health.

The invention relates to the technical field of underground water environment monitoring, and discloses a correlation analysis method for arsenic content and microbial diversity of underground water, which comprises the following steps of: placing a semi-closed diffusion buffer probe into a water-containing layer, and constructing a low Peclet number environment by using a microporous ceramic semi-permeable membrane; a substrate is injected through the microfluidic unit according to a sine rule to establish a periodic concentration field. A dual-channel electrochemical sensing array is adopted, an asynchronous stroboscopic sampling strategy is combined to respectively collect arsenic concentration and biofilm impedance signals, and a response waveform is reconstructed in an equivalent modulation period. Furthermore, the amplitude and phase of the signal are extracted through digital phase-locked amplification, a dynamic hysteresis loop is constructed, and geometric features are calculated to obtain a functional diversity index. The method effectively solves the problem that in-situ electrochemical detection consumes long time and is difficult to track fast-changing signals, and realizes dynamic correlation analysis of arsenic form transformation and microbial metabolic activity.

The invention discloses a traditional Chinese medicinal material planting land soil microbial diversity analysis method and system, and belongs to the field of soil detection and analysis, and the method comprises the following steps: when the content of effective components in target traditional Chinese medicinal materials in a target planting land is lower than a preset reference value, S1, carrying out microbial diversity analysis on the target planting land, microbial populations of the target planting land are obtained, and the soil pH value of the target planting land is detected; s2, comparing the microbial population of the target planting land with the microbial population of the genuine production area of the target traditional Chinese medicinal materials to obtain the microbial population difference between the target planting land and the genuine production area, and judging whether the soil pH value of the target planting land is within the pH value range of the genuine production area or not; s3, the soil environment of the target planting land is improved according to the microbial population difference and the pH value judgment result. Priori knowledge of a genuine production area can be fully utilized, a basis is provided for soil improvement of a traditional Chinese medicinal material planting field, and the growth quality of traditional Chinese medicinal materials is effectively improved.

This system provides an information processing system that can propose improvements to the spatial health of a given space. [Solution] An information processing system that proposes ways to improve the spatial health of a predetermined space, comprising: a spatial health calculation unit that calculates the spatial health of the space; a gap analysis unit that calculates the difference between the spatial health and a preset target value for spatial health; and an intervention means selection unit that selects an intervention means based on the difference. The spatial health is calculated based on one or more selected from a group consisting of the amount of microorganisms contained in the space, the proportion of human-derived microorganisms in the microbial community, the proportion of pathogenic microorganisms, and microbial diversity.

The invention discloses a gastric cancer risk assessment and diagnosis method based on gastric mucosa micro-ecological change, relates to the technical field of medical diagnosis and biology, and provides the gastric cancer risk assessment and diagnosis method based on the gastric mucosa micro-ecological change. The method comprises the following steps: collecting gastric mucosa tissues or gastric juice samples, extracting total DNA (deoxyribonucleic acid) of microorganisms, performing high-throughput sequencing by using an Illumina MiSeq platform, analyzing the diversity of the microorganisms, and identifying key flora. And inputting a random forest model by combining the concentration of metabolites such as short-chain fatty acid and clinical information, outputting a gastric cancer risk score, and dividing risk grades. The model is trained based on a large number of samples, and high accuracy is ensured. High-risk persons suggest gastroscope precise examination within three months, and personalized intervention suggestions are provided, so that early warning and precise health management are realized.

The invention discloses a modified carbon-based microbial agent for improving saline-alkali soil as well as a preparation method and application thereof, and relates to the technical field of biology. The modified carbon-based bacterial agent is prepared from modified charcoal, bacillus marishii BM-C55 and bacillus velezensis IHRS7, and the modified charcoal-based bacterial agent is prepared from modified charcoal, bacillus marishii BM-C55, bacillus velezensis IHRS7 and bacillus velezensis IHRS7, and the modified charcoal-based bacterial agent is prepared from the modified charcoal, the bacillus marishii BM-C55 and the bacillus velezensis IHRS7. The bacillus velezensis BM-C55 and the bacillus velezensis IHRS7 are loaded in the modified biological carbon; the modified biochar is oxalic acid modified biochar. The modified carbon-based microbial agent provided by the invention can effectively improve saline-alkali soil, and has good effects of promoting plant growth under saline-alkali stress, improving saline-alkali soil quality and increasing soil microbial diversity.

The invention provides an accurate regulation and control method and system for a water treatment biological membrane flora, and belongs to the technical field of water treatment biological membranes. The method comprises the following steps: a data acquisition step: acquiring microbial community data and environment operation parameters from a plurality of water treatment biological membrane systems; an index calculation step: calculating microbial diversity and functional characteristic indexes by using a 16S rRNA high-throughput sequencing result; a model construction step: constructing a neural network based on a structure interaction generalized additive model; an influence analysis step: obtaining the influence rate of each input variable and the variable pair on the microbial diversity and functional characteristic indexes through interpretability analysis; and a regulation and control output step: outputting a visual regulation and control suggestion based on a modeling result. The system comprises a data acquisition module, an index calculation module, an interpretation modeling module, an influence analysis module and a parameter regulation and control module. The method has the advantages of being high in interpretability, good in generalization performance, high in engineering applicability and the like, and a scientific basis and a universal method are provided for intelligent regulation and control of flora.

The application discloses a soil remediation and crop yield improvement composite material and a preparation method and application thereof, and belongs to the technical field of soil and fertilizer. The composite material comprises magnesium and aluminum, and the mass fraction of the magnesium and aluminum is 93-99.2%, and trace components, and the mass fractions of the trace components are as follows: 0.5%-1.5% of calcium, 0%-1.0% of iron, 0.05%-0.5% of copper, 0%-1.0% of zinc, 0.05%-0.4% of boron, 0.05%-0.5% of lanthanum, 0.05%-0.1% of molybdenum, 0%-1.0% of manganese and 0.1%-1.0% of silicon. The composite material of the application generates magnesium-aluminum intercalated two-dimensional nanometer functional materials in situ by hydrolysis in soil, regulates the oxidation-reduction potential of the soil, supplements trace elements, inhibits soil acidification and salinization, enriches microbial diversity, enhances the stress resistance, drought resistance and disease and pest resistance of plants, realizes the super-stable mineralization of heavy metals, the degradation of agricultural chemical residues and the yield improvement of crops.

The invention belongs to the technical field of microbial amplicon detection, and particularly relates to a method for absolute quantification of fungi in a sample by using an internal reference sequence. According to the method for absolutely quantifying the fungi in the sample by using the internal reference sequence, the absolute quantification of the fungi microorganisms is realized by designing the specific internal reference sequence, and the technical problem that only relative abundance can be obtained and the absolute content cannot be obtained in the prior art is solved. Besides, the internal reference sequence has no homology with a DNA sequence in a natural organism, so that the internal reference sequence can be accurately separated from a sequencing result, interference with other sequences in a sample is avoided, meanwhile, the internal reference sequence has similar amplification efficiency with a target sequence, and the accuracy of the internal reference sequence is improved. And quantitative deviation caused by amplification efficiency difference is reduced. In terms of operation, the method is simple to operate, accurate in detection and wide in application, and has a wide market prospect in microbial diversity research.

The invention discloses a multi-dimensional comprehensive evaluation method for microbial diversity of a hot spring, which comprises the following steps: collecting a water sample, a sediment and a biological membrane along the temperature gradient of the hot spring to form a sample, and carrying out pretreatment and in-situ fixation on the sample; carrying out metagenome, metatranscriptome and amplicon sequencing treatment on the sample; calculating three-dimensional indexes of a structural stability index SSI, a functional activeness score FAS and abundance of an environmental adaptability gene set through multi-omics integration analysis; and generating a biodiversity comprehensive evaluation report through a three-dimensional index based on the environmental adaptation index EAI. According to the method, three-dimensional quantitative analysis of structure-function-environment adaptability is realized. Through multi-omics stack analysis and environment factor coupling modeling, the problem of integrity deficiency of extreme environment biological resource evaluation is solved.

The invention discloses a method for evaluating the influence of gene editing wheat on non-target organism symbiotic microorganisms, and relates to the technical field of safety evaluation of gene editing plants on non-target organisms. The method comprises the following steps: selecting non-target organisms growing on a gene editing wheat material and a control material at the same time, analyzing diversity index difference through DNA extraction, PCR amplification and high-throughput sequencing, carrying out inter-group difference analysis by utilizing a difference detection method, and carrying out quantitative analysis and statistical analysis by adopting a method of combining qualitative analysis and statistical analysis. The effect on non-target biosymbiotic microorganisms is evaluated. For example, the diversity of non-target organism symbiotic microbial communities on the gene edited wheat and the receptor wheat has no significant difference, and the gene edited wheat has no significant influence on the diversity of microorganisms in non-target organisms. The scientific evaluation method is established, the influence of the gene editing crops on the non-target biosymbiotic microorganisms can be more comprehensively and accurately analyzed, and the method has important significance for improving environmental safety evaluation of the gene editing plants.

The invention belongs to the technical field of soil quality evaluation, particularly relates to a soil quality evaluation method based on coupling of microbial diversity and organic matter content, and aims at solving the problems that an existing method is single in evaluation dimension, insufficient in index coupling and large in result deviation. Comprising the steps of sample collection and treatment, microbial diversity and organic matter index detection, abnormal value and redundant index elimination, weight determination and standardization treatment, coupling model construction and quality grading. Microbial diversity parameters are obtained through high-throughput sequencing, the content of total organic matters and organic matters easy to oxidize and difficult to oxidize in soil is accurately determined, and the activity ratio is calculated; an analytic hierarchy process and an entropy weight method are coupled to determine an index weight, a dimensional difference is eliminated through range standardization, a model is constructed to calculate an SQI value, and grading is carried out according to a five-level standard. Double-core index deep coupling is achieved, the evaluation result is accurate, a quality short plate can be positioned, guidance is provided for targeted improvement, and an evaluation-improvement closed loop is formed.