145 results about "Microbial diversity" patented technology

The invention discloses a bacillus megaterium strain X3 and application thereof to plant growth promotion. The bacillus megaterium strain X3 is preserved in the China General Microbiological Culture Collection Center (CGMCC) on May 11, 2015 with the preservation number of CGMCC No.10803, is obtained through separation of indoleacetic acid, has the characteristics of siderophore production, phosphate solubilizing, ammonia producing, salt tolerance and disease suppression, can remarkably promote crop growth and improve corn yield, and meanwhile, can increase the diversity and the abundance of microbes in soil. The bacillus megaterium strain X3 is quick in reproduction, simple in production process, strong in adversity ability, easy to preserve and conductive to industrial production, and can not only prevent and cure soil-borne diseases but also promote plant growth when utilized to prepare a bio-fertilizer, thereby having a wide application prospect.

The invention discloses a computation cloud platform-based microbial diversity interaction analysis system and method thereof. The system comprises a project management module, a basic analysis task submission module and an interactive result analysis module. The method comprises the following steps of: firstly uploading sequencing data to a local cluster server, establishing a project in the project management module, uploading a database of customers to the local cluster server or selecting to use an online database, and locking the project in the project management module or sharing the project to other users to be operated; in the basic analysis task submission module, carrying out parameter analysis on the sequencing data by a user through a visual interface and generating a project file after the analysis; before the analysis, judging whether data quality control is qualified or not, if the judging result is positive, carrying out the parameter analysis, and otherwise, directly returning an error; and transmitting the generated project file to the interactive result analysis module to carry out interactive analysis until an intuitively displayed report is obtained.

The disclosure relates to improving plant breeding methods by controlling for microbial diversity present in the plant breeding process.

The invention relates to a method for simultaneously improving sludge anaerobic digestion efficiency and reducing the ecotoxicity of heavy metals. According to the method, after straws are activated with a ZnCl2 solution, straw biochar is prepared under a 550 DEG C high-temperature anaerobic condition, and further modification is performed by using an HCl solution and microwave; and by using modified biochar as carrier, a zero-valent nano-iron loaded modified biochar composite material HCl-FBC is prepared through a chemical reaction. According to the present invention, by adding the modified biochar composite HCl-FBC to the anaerobic digestion reactor at the initial stage of sludge anaerobic digestion, the organic matter conversion rate of sludge can be significantly increased, and the cumulative gas production of methane can be increased; HCl-FBC can easily increase the microbial diversity and the activity of the digested sludge and change the microbial community structure; and with the method, the sludge anaerobic digestion efficiency can be significant increased, the biogas production can be increased, the biological toxicity of the heavy metal in the digested sludge is effectively reduced, and the great significance is provided in the resource utilization of sludge and the reduction of the risk of sludge land use.

The invention discloses a multifunctional soil improver and a preparation method thereof. The preparation method comprises the following steps: 1) preparing fermented livestock and poultry manure; 2) preparing an organic ecological fertilizer; and 3) preparing a soil improver. The multifunctional soil improver disclosed by the invention has the beneficial effects that raw materials adopted by the method are byproducts or waste slag materials in industrial and agricultural production, so that not only is price low, but also complete utilization of resources is realized, and the environment is protected; soil can have good water holding capacity, soil bulk density also can be reduced, porosity is improved, physiochemical properties of the soil are improved, more soil nutrients are retained, pH value is regulated, and soil fertility and fertilizer maintenance capability are improved; organic compounds are provided, so that enzyme activity and nitrogen and phosphorus cycle in the soil are improved for improving microbial diversity and microbial activity, quantity of beneficial bacteria is increased, pathogenic bacteria can be killed, migration capability of heavy metals can be reduced, and absorption of crops on the heavy metals is inhibited, so that the multifunctional soil improver disclosed by the invention is efficient, practical and environment-friendly.

The invention discloses a method for cooperatively repairing chromium polluted soil by biochar-based zero valent iron and microorganism, and relates to methods for repairing soil environments. The method for cooperatively repairing the chromium polluted soil by the biochar-based zero valent iron and microorganism aims to solve the problem that agglomeration and oxidation are prone to occurring easily when the chromium polluted soil is repaired. The method for repairing the chromium polluted soil includes the steps that the biochar-based zero valent iron is prepared, specifically, biomass is added to a soluble iron salt solution for modification, the modified biomass is filtred and roasted to obtain a biochar-based zero valent iron remediation material; strain domestication is carried out,specifically, an organic matter and hydrogen are selected from the chromium polluted soil as electron donors, hexavalent chromium, trivalent iron, sulfate, nitrate and oxygen are selected from the chromium polluted soil as dominant bacteria of electron acceptors for metabolism activities to be subjected to domestication and expanding culture; and repairing is carried out, specifically, the biochar-based zero valent iron, the dominant bacteria and the chromium polluted soil to be repaired are mixed at room temperature for repair. According to the method for cooperatively repairing the chromiumpolluted soil by biochar-based zero valent iron and microorganism, the reduction rate of the hexavalent chromium can be greatly improved, the mobility and bioavailability of chromium are reduced, thesoil can further be improved and the microbial diversity can be increased.

The invention relates to the field of organic agricultural production and soil fertility improvement, in particular to a method for rapidly improving organic agricultural soil fertility and application thereof. The method for rapidly improving organic agricultural soil fertility, provided by the invention, is that a compound conditioner is applied to the soil and earthworms are released to the soil, wherein before the compound conditioner is applied and the earthworms are released, the soil is required to meet the following conditions: 1), the soil moisture content is kept to be 30%-50%, and 2), soluble-salt content of the soil is kept to be lower than 0.04%; 300-500 kg of the compound conditioner is applied to per mu of soil; 30-50 kg of the earthworms are released to per mu of the soil. By adopting the method provided by the invention, the number of earthworms in a farmland is increased greatly, the activity of earthworms in the farmland is improved greatly, soil rejuvenation and fertility improvement rate is increased, microbial diversity of the soil environment is increased, soil quality is improved, crop quality and yield are improved and sustainable development of organic agriculture is realized.

The invention relates to the water treatment field and specifically relates to a water treatment method for purifying sewage by use of microorganisms. The water treatment method is characterized by comprising at least the steps of arranging at least one biochemical bed in a water area to be treated and arranging a frame with a through opening on the biochemical bed. The water treatment method has the advantages that excellent microbial slow release effect can be produced, the microbial diversity in the water area is greatly increased, the reproduction and growth environment for the microorganisms is greatly improved and the influence of activity decline of the microorganisms in winter is counteracted, and the addition volume ratio of the biochemical bed relative to the water area to be treated is greatly lower than that of a filler in a conventional biochemical process.

The invention discloses a liquid fertilizer capable of preventing soil-borne diseases. The liquid fertilizer is prepared from the following raw materials, by weight, 36-40 parts of biogas residue mud, 5-7 parts of sodium potassium tartrate tetrahydrate, 6-8 parts of citric acid, 40-50 parts of corn germ meal, 15-19 parts of urea, 55-65 parts of dry chicken manure, 120-140 parts of waste livestock and poultry feathers, 6-7 parts of copper sulphate, 4-6 parts of ferrous sulphate, 4-5 parts of GM microbial preparations and a proper amount of water. Biogas residue mud, chicken manure and the like are employed in the raw materials of the liquid fertilizer and contain a lot of microbes with various kinds, the GM microbial preparations are added to raise the beneficial bacterium content, thus the liquid fertilizer becomes a microbe resource base, the soil microbial community structure can be adjusted after drip irrigation and spraying of the liquid fertilizer, the soil microbial diversity is raised, the soil micro-ecological environment is improved, the survival scope of plant pathogens is narrowed, and therefore the purpose of preventing soil-borne diseases is achieved.

The invention provides a feces preservation solution, a preparation method thereof and a feces preservation method. The feces preservation solution contains 0.10-0.20 M of Tris base, 0.25-0.75 M of ethylenediaminetetraacetic acid (EDTA), 10 mM-room temperature saturated sodium chloride (NaCl), 5-15% of dimethyl sulfoxide (DMSO), 2.5-10% of ethanol and 5-20% of glycerol, wherein the pH of the fecespreservation solution is 7.0-9.0. The feces preservation solution can stably preserve microbial diversity in feces samples at normal temperature and improve DNA concentration, can ensure the accuracyof sequencing analysis of intestinal flora, realizes normal-temperature preservation and transportation of the feces samples, has a good preservation effect compared with an existing preservation solution, does not contain toxic components such as guanidine thiocyanate, and is safer to operate.

The present invention relates to a method for evaluating water quality by using microbial diversity indicators in water sediments. According to the method, the structure of microbiological community in sediments is determined by using the Miseq high throughput sequencing, the relationship between the microorganism and the living environment is researched by using such statistic methods as principal component analysis, and non metric multidimensional scaling analysis, and the PA and BGN are calculated, wherein beta evaluates microbial indicators to determine the water eutrophication level and pollution level. According to the present invention, the microbial community characteristics of different types of water sediments in different pollution types can be objectively and comprehensively obtained by using the high throughput sequencing technology, water bodies and water qualities are evaluated integrally, and the selected microbial evaluation indicators can sensitively reflect the change of the water quality and the adaptability is wide, which can accurately detect all types of water sediments.

The invention relates to a denitrifying accelerator and application thereof. The denitrifying accelerator comprises the following components in percentage by mass: 75%-80% of polyhydric alcohols, 0. 7%-2.25% of mineral elements, 0.5%-1.0% of organic acid, 1.0%-2.5% of biological enzyme, and the balance of water, wherein the molecular weight of polyhydric alcohols is less than or equal to 100. Thedenitrifying accelerator is used for wastewater treatment, can achieve relatively high denitrification efficiency with little amount, can promote microbial diversity, is nonpoisonous and harmless, canbe added directly and conveniently through a metering pump without special protective measures, and also is less prone to inflammation and explosion and requires no extra explosion protection zone for storing carbon sources so as to save construction investment; at the same time, the denitrifying promoter has liquid carbon source with low freezing point, and thus does not condense even when not stored indoors in winter.

The invention discloses a total DNA (Deoxyribonucleic Acid) extraction method of bottom mud containing high content of humus at the river mouth. The method comprises the following steps: washing bottom mud by stroke-physiological saline solution to remove part of impurities such humus, and meanwhile, concentrating microorganisms; then, multigelating in liquid nitrogen and a water bath at 65 DEG C by means of CTAB (Cetyltrimethyl Ammonium Bromide), proteinase K and lysozyme for cell disruption, and then adding SDS (Sodium Dodecyl Sulfonate) and insulating for 2 hours in the water bath at 65 DEG C to combine protein; adding isovolumetric phenol / chloroform / isoamylol (25:24:1) to remove protein; adding isovolumetric isopropanol to set DNA, washing precipitate with 70% ethanol, and dissolving DNA by TE (pH8.0) after airing; adding 3 times of absolute ethyl alcohol in volume to re-set DNA, washing the precipitate with 70% ethanol, dissolving the precipitated DNA by TE after airing, and storing at minus 20 DEG C for later use. The method provided by the invention is simple to operate and cheap in price. The total DNA of bottom mud extracted by the method provided by the invention has the advantages of high purity and integral biodiversity, and can be used for molecular biology study and microbial diversity analysis.

The invention provides bacillus coagulans for improving growth performance of white feather broiler. The bacillus coagulans is separated from a digestive tract of healthy poultry; the research shows that the bacillus coagulans is added in daily ratio of the white feather broiler, so that the number of escherichia coli and the ammonia nitrogen concentration in cecum of the white feather broiler, and the microbial diversity index of cecal digesta is improved; besides, the research result shows that by feeding the bacillus coagulans, the mortality and elimination rate and the feed conversion rate of the white feather broiler are improved, and the daily grain of the boilers in experimental group is improved.

The invention discloses lysinibacillus fusiformis and an application thereof in land reclamation and ecological reconstruction in a mining area. The strain is the lysinibacillus fusiformis, and has acollection number of CGMCC No. 9799. The strain is capable of improving the nutrient content of land reclamation soil in the mining area, and has obvious effects for improving the content of organic matters, alkali-hydrolyzale nitrogen, rapidly available potassium and available phosphorus in the land reclamation soil in the mining area. Moreover, further experiments prove that, the lysinibacillusfusiformis is capable of further improving the biomass of crops, and for culture of wheat or oilseed rapes, the biomass of crops in the land reclamation soil in the mining area can be improved; particularly important, due to use of the lysinibacillus fusiformis, the community structure of soil microorganisms can be effectively and obviously changed, the microbial diversity of the soil is improved,and the quantity of microbes is effectively increased. Therefore, remediation of the microbial community in the soil is accelerated, and the lysinibacillus fusiformis can be widely applied to remediation of the land reclamation soil in the mining area.

A method of interfering with quorum sensing regulation of genes to promote cell growth is disclosed. The method of is aimed at accessing microbial biodiversity. The method involves obtaining an environmental sample comprising at least one novel (uncultivated in the laboratory) microorganism, contacting the environmental sample with an effective amount of an agent or combination of agents which interferes with the quorum sensing regulation of genes, growing the treated sample in a culture medium containing the quorum sensing signal disrupting agent or agents, and analyzing the colonies of microorganisms grown to demonstrate genetic novelty.

The invention discloses a computing cloud platform-based microbial diversity interactive analysis system and method. The system comprises a project management module for viewing and managing project information, a basic analysis task submission module which is used for setting a basic parameter operation task, integrating and packaging results and original data into a corresponding project file according to a preset format, and an interactive result analysis module which is used for generating an analysis result according to a user request and visually displaying the result. According to the system and method of the present invention, a highly integrated data analysis platform is provided by means of cloud computing technology, and a good foundation is laid for basic analysis, advanced analysis and personalized analysis covering microbial diversity analysis.

The invention relates to the field of water treatment and concretely relates to a method for water environmental modification by a microbial bag. The method at least comprises arranging at least one biochemical bed in a water area and arranging a frame with a through port on the biochemical bed. The method has a good microbe slow release effect, greatly improves microbial diversity in a water area, greatly improves a microbe breeding and growth environment, and eliminates influence of reduction of microbial activity in winner. A use volume ratio of the biochemical bed to treated water is greatly lower than a use volume ratio of the conventional biochemical process filler.

The invention relates to a general and reliable extraction method of total DNA of soil and application thereof, belonging to the field of molecular biology. The soil contains very rich microbial resources, but if the traditional pure culture technique is utilized, only 1 percent of microbes can be cultured, while the microbes incapable of being cultured are the main body of microbial diversity of the soil, thus bringing great limitation to the development and utilization of the diverse resources of the microbes. The extraction method provided by the study uses solution I, solution II, potassium acetate and magnesium chloride to remove the impurities of humic acid and the like, can meet the needs of different types of soil samples, can obtain DNA which has high quality and large fragment and is suitable for following cloning experiment, and lays the foundation for studying the microbial diversity of the soil (sludge) sample in various environments. The extraction process does not need to be purified, thus shortening the extraction step and reducing the extraction cost.

The inventions described herein relate generally to digestive healthcare, and more particularly, to the feeding of mammals, particularly human infants, who are making a transition from a microbiome with lower diversity to a microbiome with higher diversity. These inventions relate to certain foods comprising a fermentable nutritional component and a probiotic component, where the probiotic component is selected, based on genetic and / or metabolic criteria, to specifically metabolize any Free Sugar Monomers (FSMs) and Free Amino Acids (FAAs) or peptides that accumulate as a result of the fermentable nutritional component in the lower intestine, where they otherwise might be left in the environment to be fermented and metabolized by less adapted / opportunistic bacteria, creating blooms of deleterious intestinal bacteria and shifting the microbiome to a potentially dysbiotic state. The present inventions provide combinations of foods and probiotic bacteria that can protect the mammalian gut from blooms of pathogenic bacteria under the circumstances where the mammalian gut is starting out with a low microbial diversity, such as in weaning infants, or individuals who are post-antibiotic treatment and / or post-chemotherapeutic treatment and transitioning to a higher diversity adapted / stable microbiome.

The invention discloses a microbial strain organic fertilizer for improving soil alkalinity. The strain in the organic fertilizer can produce alkali under different fermentation conditions, and can be used against a soil acidification problem of over-farmed soil. Bacillus can survive and grow under stresses, can provide various enzymes for soil, and assists in improving soil microbial diversity. An alkali-producing bacillus is screened and is applied in acidic soil, and crop rhizosphere micro-ecology of the acidic soil can be tuned and optimized. Soil urease activity can be improved, such that an excellent liquid microbial fertilizer is provided for the growth of crops such as Indian lettuce and lettuce in the acidic soil. With the organic fertilizer prepared with the strain HNPY23, leaf tissue chlorophyll content, leaf Vc content and yield of the crops such as Indian lettuce and lettuce can be substantially improved. With the fertilizer, both quality and yield can be improved.

The invention discloses an adsorption ball for organic pollutants in water, and relates to the field of water pollutant removal. The adsorption ball comprises a spherical framework made of biodegradable materials, the spherical framework is internally provided with a cavity, pores are uniformly distributed in the surface of the framework, and the cavity is filled with activated carbon, ceramic particles, activated sludge and zeolite particles. By means of the scheme, the three main pollutants, including organic matter, ammonia nitrogen and suspended algae, in the water can be comprehensively removed, and various main pollutants in the water is effectively removed; meanwhile, the adsorption ball is also a high-efficiency microbial carrier for algal-bacterial symbiosis; the establishment ofa natural water ecosystem is accelerated; the microbial diversity is improved.

The invention relates to the technical field of biology, in particular to application of melatonin serving as an effective component of a foliar spraying agent to influence on rhizosphere soil microorganism diversity of medicinal plants. The content of the melatonin in the foliar spraying agent is 100-300 mg / L, and an inducer further contains 0.05 g / L of hydroxypropyl methyl cellulose. The melatonin can reduce the content of endogenous salicylic acid and hydrogen peroxide of plants, increase the content of jasmonic acid, promote proliferation and endosymbiosis of rhizosphere probiotic fungi and change the rhizosphere soil microorganism diversity of the paris polyphylla. In conclusion, when the melatonin is sprayed on the leaf surfaces, the composition of rhizosphere microorganisms can be adjusted by changing the endogenous hormone level, so that the effects of resisting diseases and promoting growth of the paris polyphylla are achieved. The foliar spraying agent taking the melatonin as the effective component is used for cultivating paris polyphylla var chinensis and has the effects of resisting diseases and promoting growth, the preparation and use methods are simple, the cost is low, and popularization and application can be realized.

The invention relates to the technical field of crop rotation culture, in particular to a wheat, oilseed rape and maize crop rotation cultivation method. The method comprises the steps that wheat, oilseed rape and maize crop rotation is performed in a field, due to the oilseed rape planting water-logged compost link, the application amount of chemical synthesis fertilizer can be greatly lowered, the current situation that two cropping systems of crops are not sufficient and single cropping is abundant in a Northern loess plateau dry land planting area is changed, due to the water-logged compost link, the soil section nitrogen supply capability can be improved, the available phosphorus, available iron and manganese contents are effectively increased, and the activities of sucrose, urease, alkaline phosphatase and proteinase are improved to different extents; compared with continuous cropping, due to crop rotation is performed on green manure crops of growing after wheat, the soil microbial diversity can be remarkably improved, the stability of an agroecological system is affected, the soil carbon cycling and phosphorus mobilization are accelerated, and the effective soil nutrient content is increased; meanwhile, the soil nitrogen mineralization rate is lowered, and stability and accumulation of soil nitrogen are promoted, so that the soil fertility level is increased, and the application amount of the chemical fertilizer is decreased.

The invention relates to a genital tract flora nucleic acid preservation solution formula. A genital tract flora nucleic acid preservation solution comprises a Tris-HCl buffer solution with the concentration of 0.01-1 M, ethylenediaminetetraacetic acid with the concentration of 0.01-1 M, sodium chloride with the concentration of 0.01-0.5 M, sodium citrate with the concentration of 0.01-0.5 M and 10-30% anhydrous ethanol. The pH value of the prepared preservation solution is 8.0-10.0. The preservation solution is prepared through the method and used for collecting a genital tract sample, has the advantages of protecting microorganisms and nucleic acids thereof in the genital tract from being degraded, protecting the microbial diversity, facilitating collection and transportation of the genital tract sample and facilitating downstream gene detection, is beneficial to genital tract flora diversity research, and is applied to gene detection by taking genital tract floras as target points.

The invention discloses a composite type sterilizing agent composition for treating industrial water. Sterilizing agents of the composite type sterilizing agent composition comprise, by weight, 50-90 parts of bromine compounds, 40-70 parts of amphoteric sterilizing agents, 1-15 parts of dispersing agents, 1-5 parts of inorganic acid, 0.5-3 parts of polymerization inhibitors, 5-10 parts of potassium hydroxide and 10-50 parts of deionized water. The composite type sterilizing agent composition has the advantages that the bromine compounds and the amphoteric sterilizing agents are compounded with one another, potential of various components can be played to the greatest extent under the synergistic effects, and accordingly requirements on microbial diversity sterilization treatment on the industrial water can be met; the sterilizing agents are low in toxicity and easy to degraded, and corrosion inhibition sterilization effects can be realized.

The present invention pertains to a composition comprising garcinol individually or in combination with forskolin for modifying the gut microbial diversity. More specifically, the invention relates to the use of composition comprising garcinol and forskolin for decreasing the Firmicutes / Bacteroidetes ratio and increasing the count of beneficial microbe, Akkermansia muciniphila.

The invention relates to an oil-reservoir endogenous microbe in-situ recovery method. The method comprises the following steps: 1, well ore data analysis: collecting and organizing base data of a target well; 2, value simulation: determining microbial recovery environment adaptability requirements based on a concrete design scheme required by the microbial enhanced oil recovery; 3, determining a sample burying depth through the well ore analysis data; 4, in-situ recovery: recovering required endogenous microbial resources on the scene, and recovering an oil sample or an oil water sample fluid at a specific oil reservoir target burying depth through a PVT (pressure, volume and temperature) instrument according to the value stimulation result; and 5, carrying out subsequent microbial diversity analysis and screening. The method is suitable for the endogenous microbial recovery under various temperature, pressure, pH, mineralization, burial depth oil-reservoir geology conditions, is especially suitable for the deep-well microbial recovery of high-temperature and high-pressure oil reservoirs, and has uses comprising the trace monitoring of oil-reservoir harmful microbes, the endogenous / exogenous microbial diversity analysis before and after the action of the microbial enhanced oil recovery, and the like.

The invention discloses a method for regulating and controlling a field implementation effect of indigenous microbial oil displacing. The method comprises the following steps that field sampling of anoil reservoir is conducted; pretreatment of samples is conducted; an indigenous microbial community structure is analyzed; indigenous microbial diversity is calculated; a preliminary regulating and controlling scheme is determined; a final regulating and controlling scheme is determined; and field implementation is conducted. The method for regulating and controlling the field implementation effect of indigenous microbial oil displacing has the characteristics of being reasonable in method, simple in technology, simple in operation, safe and reliable, low in input and low in cost, meanwhile,production performance can be regulated and controlled during the field implementation process in a targeted mode, the responding period of a field test of indigenous microbial oil displacing is effectively prolonged, and the field test effect is improved; and therefore, the method for regulating and controlling the field implementation effect of indigenous microbial oil displacing can be widely applied to the field test of improving the recovery efficiency of indigenous microbial oil displacing.

The invention relates to the technical field of molecular biology and particularly relates to a construction method and an application of a microbial diversity library. According to the novel construction method of the microbial diversity library, the microbial diversity library of a metagenome is constructed by virtue of single chain linkage and a special label primer joint; the scheme is applicable to conventional samples, trace samples, FFPE samples and less than 150 cell groups, and the application range of microbial diversity research is extended; and by introducing the single chain linkage and a special label primer, different target populations fragments can be enriched in a small deviation manner, so that the richness of species and the authenticity of abundance of different species are guaranteed. Besides, the construction method further has the advantages that the initial mass is low, and the same sample can be subjected to multi-species authentication.