1051 results about "Proline" patented technology

To provide a compound which is a novel proline derivative, and to provide a pharmaceutical composition comprising the compound for the treatment of, for example, systemic sclerosis (SSc) and non-alcoholic steatohepatitis (NASH).SOLUTION: A pharmaceutical composition comprises, as a cGAS inhibitor, a compound which is a novel proline derivative encompassed by formula (I), or a prodrug, a pharmaceutically acceptable salt, or a stereoisomer of the compound.SELECTED DRAWING: None

The invention belongs to the technical field of gene engineering, and particularly relates to applications of OpgG and a mutant thereof in production of human milk oligosaccharide. The invention provides a dextran biosynthetic protein OpgG mutant, and the OpgG mutant is obtained by mutating the 443rd amino acid from threonine to proline on the basis of a wild type dextran biosynthetic protein OpgG as shown in SEQ ID NO.1. The invention also provides a preparation method of the dextran biosynthetic protein OpgG mutant. An opgG gene and / or a T443P mutant coding gene are / is introduced into a human milk oligosaccharide production strain and applied to production of human milk oligosaccharide, and the yield of 3 '-SL, 6'-SL, LNT II, LNnT and LNT is greatly increased.

The invention discloses a formate dehydrogenase mutant and application thereof in catalytic synthesis of D-mannitol, and belongs to the technical field of bioengineering. According to the formate dehydrogenase mutant provided by the invention, the 18th-site glutamic acid of wild-type formate dehydrogenase with an amino acid sequence shown as SEQ ID NO.2 is mutated into proline, the 57th-site asparagine is mutated into glutamic acid, the 70th-site histidine is mutated into tryptophan, the 235th-site serine is mutated into threonine, and the 316th-site valine is mutated into threonine, so that the formate dehydrogenase mutant is high in enzyme activity and catalytic activity; the coenzyme circulation efficiency during the production of D-mannitol can be obviously improved, so that the yield of D-mannitol can be effectively improved. When the genetically engineered bacterium co-expressed by the formate dehydrogenase mutant and the mannitol dehydrogenase mutant is used for catalyzing 150 g / L fructose to produce D-mannitol, the yield of D-mannitol within 15 h can reach 140 g / L or above.

The invention discloses a method for efficiently screening saline-alkaline tolerant rice. According to the method, controllable indoor saline-alkali environment simulation conditions are constructed, various salts are mixed in proportion to prepare solutions with different salinity gradients, the pH value is adjusted, and temperature, humidity and illumination parameters are accurately controlled; the method comprises the following steps: disinfecting rice seeds, carrying out a germination test, transplanting rice seedlings, and regularly measuring growth indexes; in the treatment process, physiological indexes such as malondialdehyde content, antioxidant enzyme activity, proline content and the like and the expression level of salt and alkali tolerance related genes are detected, key indexes are screened by applying statistics and multivariate analysis methods, and an evaluation system is established for comprehensive score screening. The method can quickly perform saline-alkali stress treatment on a large number of rice seeds or seedlings, is not limited by natural environment, shortens the screening period, can accurately simulate different saline-alkali soil environments, scientifically evaluates the saline-alkali tolerance of rice from multiple dimensions, and provides an efficient and reliable technical means for breeding saline-alkali tolerant rice varieties.

The invention relates to the technical field of biological extraction, in particular to a green synergistic extraction system and an extraction method and application thereof.The green synergistic extraction system comprises a deep eutectic solvent system, an enzymolysis system and an ultrasonic-assisted extraction system, and the deep eutectic solvent system comprises a DES solvent; the DES solvent is composed of a hydrogen bond acceptor and a hydrogen bond donor, and the hydrogen bond acceptor is choline, choline chloride or L-proline; the hydrogen bond donor is lactic acid, urea or ethylene glycol; enzyme preparations in the enzymolysis system are at least two of cellulase, pectinase and beta-glucosidase; the problems that a traditional organic solvent is high in toxicity and poor in biodegradability and synthetic deep eutectic solvent raw materials are non-renewable are solved, a green synergistic extraction system which is environmentally friendly, low in cost and excellent in performance is developed, the mass transfer efficiency and the extraction yield are improved, the used DES and enzyme preparations can be recycled, and the cost is reduced while the environment is protected.

The invention discloses a formate dehydrogenase mutant and application of the formate dehydrogenase mutant in catalysis of carbon dioxide reduction, the 223 arginine (Arg) of an amino acid sequence of formate dehydrogenase PsFDH48 derived from Paracoccus sp. MKU1 is mutated into proline (Pro) or the 242 proline (Pro) is mutated into phenylalanine (Phe) through a site-specific mutagenesis technology, and the obtained mutants R223P and P242F have higher catalytic activity and can be applied to catalysis of carbon dioxide reduction. The catalyst can catalyze CO2 to generate formic acid more effectively in vitro, and has potential application value in the aspects of efficient activation of CO2 and further conversion of CO2 into other mono-carbon compounds through cascade reaction.

The invention provides a plant lactobacillus TH13, and the preservation number of the plant lactobacillus TH13 is CGMCC (China General Microbiological Culture Collection Center) NO.32112. The plant lactobacillus TH13 provided by the invention can be used for fermenting folium cortex eucommiae and enzymatically hydrolyzed bone meal, and the fermentation product of the plant lactobacillus TH13 has high content of chlorogenic acid, proline, glycine and lysine. The fermentation product can significantly improve the content of alkaline phosphatase (ALP) and osteocalcin (OCN) in mouse serum and reduce the content of tartrate-resistant acid phosphatase (TRACP). Therefore, the fermentation product can significantly improve the bone mineral density of the femur, increase the number of bone trabecula and significantly reduce the separation degree of the bone trabecula, thereby promoting bone formation of a mouse with senile osteoporosis, inhibiting bone resorption and improving senile bone loss and bone microstructure deterioration. The preparation method of the probiotic preparation containing the plant lactobacillus and the probiotic fermentation product is simple, and large-scale production can be realized.

The invention belongs to the technical field of cosmetics, and discloses a wrinkle-removing and anti-aging composition containing recombinant collagen as well as a preparation method and application of the wrinkle-removing and anti-aging composition. The composition is prepared from the following components: 20.4 to 35.6 percent of glycerol, 1.1 to 3.5 percent of 1, 2-hexanediol, 8.2 to 15.4 percent of apple fruit cell culture extract, 1.6 to 3.2 percent of inositol, 0.2 to 1.0 percent of elastin, 0.1 to 1.7 percent of hydroxyproline, 0.1 to 1.0 percent of sodium polyglutamate, 0.1 to 1.0 percent of sodium hyaluronate, 0.01 to 0.1 percent of acetyl hexapeptide-8, 0.01 to 0.1 percent of fibronectin, 1.72 to 3.21 percent of soluble collagen and the balance of deionized water. The amino acid sequence of the soluble collagen is as shown in SEQ ID NO. 1. The skin care product prepared by adding the anti-wrinkle and anti-aging composition is good in safety and has the effects of resisting wrinkles, improving skin elasticity and resisting aging.

To provide a compound which is a novel proline derivative, and to provide a pharmaceutical composition comprising the compound for the treatment of diseases, for example, systemic lupus erythematosus.SOLUTION: A pharmaceutical composition comprises, as a cGAS inhibitor, a compound which is a novel proline derivative of formula (I) (where R1, R2, R3, R4, R5, R6, R7, R8, R9, R10, R11 and G are as defined in claim 1), or a prodrug, a pharmaceutically acceptable salt, or a stereoisomer of the compound.SELECTED DRAWING: None

The invention discloses a method for culturing CIK (Cytokine-Induced Killer) cells and application thereof. The method comprises the following steps: recovering cord blood, mixing and centrifuging the recovered cord blood and a buffer solution, re-suspending and precipitating, centrifuging again to obtain cord blood mononuclear cells, inoculating the cord blood mononuclear cells into an activation culture medium, and culturing to obtain the CIK cells, the buffer solution contains L-proline, human recombinant DNA enzyme and angelica polysaccharide. According to the method, the mononuclear cells can be separated from the umbilical cord blood preserved by liquid nitrogen, the utilization rate of the umbilical cord blood which can only be discarded due to insufficient cells is increased, the traditional lymphocyte separation process is optimized, the recovery rate of the mononuclear cells is increased, and the cost is reduced. The method provided by the invention can be used for culturing dozens to ten billions of CIK cells from a small amount of cryopreserved cord blood, and has huge advantages in the field of CIK cell culture and application.

The invention relates to a composite microbial inoculant for soil improvement, the composite microbial inoculant for soil improvement is composed of PGPR (Pseudomonas tianjinensis) and AMF (Amplified Macrophage Fungi), the PGPR is Pseudomonas tianjinensis and Pseudomonas oryzae, and the AMF is rhizophagus clarus. The composite microbial inoculant for soil improvement is characterized in that the PGPR is Pseudomonas tianjinensis and Pseudomonas oryzae, and the AMF is Pseudomonas oryzae, the PGPR is Pseudomonas tianjinensis and Pseudomonas oryzae. According to the invention, the Pseudomonas tianjinensis, Pseudomonas oryzae and AMF are compounded, so that a synergistic effect is exerted, an obvious growth promoting effect on the ryegrass in the saline-alkali soil is achieved, the content of photosynthetic pigments in the ryegrass is increased, the contents of soluble sugar, soluble protein, proline and malondialdehyde in the ryegrass are further increased, and the CAT enzyme activity is improved. In addition, the complex microbial inoculant is combined with ryegrass planting, a plant-microorganism synergistic system is formed, salinized soil is effectively improved, the conductivity and pH of the soil are reduced, the content of nitrogen, phosphorus and potassium in the soil and the content of rapidly available phosphorus and rapidly available potassium are increased, and meanwhile the activity of urease and phosphatase is improved.

The invention provides a tree species screening method for high-altitude drought afforestation, an afforestation method and application, and belongs to the technical field of forest tree breeding and cultivation. According to the screening method, five physiological and biochemical indexes of soluble protein, malondialdehyde, proline, peroxidase and soluble sugar in the tree species are measured through a gradient drought stress experiment, and comprehensive scoring is performed by adopting a membership function, so that the drought-resistant tree species are scientifically screened. The tree species such as sophora moorcroftiana, rosa serrulata and amorpha fruticosa screened by the method are applied to afforestation in the high-altitude arid region, and the afforestation survival rate and preservation rate can be remarkably increased. The invention provides a reliable technical scheme for solving the long-term problem that the selection of tree species in the difficult site field lacks scientific basis.

The invention discloses modified hydroxytyrosol as well as a preparation method and application thereof. The provided preparation method of the modified hydroxytyrosol comprises the following steps: in the presence of an acidic solvent, mixing hydroxytyrosol, protease and collagen for reaction, mixing a reaction product with an extraction agent, performing centrifugal layering, and collecting upper-layer components, thereby obtaining the modified hydroxytyrosol, the protease comprises one or two of neutral protease and trypsin. Under the enzymolysis action of protease, the collagen can be decomposed into amino acid monomers such as glycine, proline and hydroxyproline, and the amino acid monomers can be subjected to graft modification with hydroxytyrosol; meanwhile, collagen fragments which are not subjected to enzymolysis also exist, and are subjected to graft modification with hydroxytyrosol in the form of collagen characteristic fragments. The oxidation resistance and the stability of the obtained modified hydroxytyrosol are obviously improved compared with those of unmodified hydroxytyrosol.

The invention discloses a polypeptide with a whitening effect and a preparation method and application thereof.The polypeptide is obtained from hydrolyzed protein of babylonia areolata, the amino acid sequence of the polypeptide is cysteine-arginine-proline-threonine-cysteine-serine-arginine-leucine-alanine-cysteine, and the amino acid sequence of the polypeptide is cysteine-arginine-proline-threonine-cysteine-serine-arginine-leucine-alanine. The compound can obviously inhibit the generation of melanin in cells and the activity of tyrosinase in vitro, has the characteristics of low toxicity, low irritation, good stability, safety and high efficiency, and can be applied to whitening cosmetics.

The invention relates to an application of a nano silicon dioxide combined flora in relieving plant salt stress, which comprises the following steps: applying nano silicon dioxide to soil in which soybeans are to be planted, then planting the soybeans in the soil, and adding a simplified salt-tolerant growth-promoting synthetic flora microbial inoculum; wherein the synthetic flora is separated from saline-alkali soil, the growth promoting functions of nitrogen fixation, phosphorus dissolving, potassium dissolving and the like of the synthetic flora are measured, and the growth promoting effect of a single bacterium is verified through a pot experiment. A simplified flora SynC1 is constructed, and the relieving effect of combined application of the nano silicon dioxide and the flora on soybean salt stress is evaluated through a pot experiment. It is found that combined application of nano-silica and SynC1 can better promote root development, increase the proline content and promote soybean growth under salt stress, and has excellent application potential in agricultural soybean production.

To provide compounds as new proline derivatives, and to provide a pharmaceutical composition comprising the compounds for the treatment of diseases such as systemic lupus erythematosus, systemic sclerosis, non-alcoholic steatohepatitis, interstitial lung disease and idiopathic pulmonary fibrosis.SOLUTION: There are provided new proline derivatives of formula (I) as cGAS inhibitors, or pharmaceutical compositions comprising a prodrug, a pharmaceutically acceptable salt, or a stereoisomer of the compound.SELECTED DRAWING: None

The application discloses an acyltransferase mutant with high acetylation activity and application thereof, and belongs to the technical field of enzyme engineering. The acyltransferase mutant provided by the application contains one or more mutation sites in the 62th and 64th positions on the basis of the acyltransferase mutant with the amino acid sequence shown in SEQ ID NO. 1. The acyltransferase mutant obtained by the application has higher acyltransferase activity and catalytic efficiency than the original enzyme before mutation, and can improve the yield of N-acetyl-trans-4-hydroxyproline. Therefore, the acyltransferase mutant of the application has important significance in the efficient biosynthesis of N-acetyl-trans-4-hydroxyproline, and has wide application prospects in the fields of biological materials, medicines or cosmetics.

The invention provides collagen tripeptide liquid with moisturizing and anti-photoaging effects and a preparation method thereof, and relates to the technical field of bioactive peptides.The preparation method comprises the steps that pretreatment and first-time enzymolysis are conducted, specifically, pretreated fish skin and water are mixed, the pH value is adjusted, compound protease is added for enzymolysis, and first enzymatic hydrolysate is obtained; second-time enzymolysis: adjusting the pH value of the first enzymatic hydrolysate to 6.0-7.5, adding neutral protease for enzymolysis, and then adding aminopeptidase for enzymolysis for 20-40 minutes to obtain second enzymatic hydrolysate; and performing enzyme deactivation, decoloration, separation and purification to obtain collagen tripeptide liquid. Wherein the content of collagen tripeptide, the content of glycine-proline-hydroxyproline and the content of hydroxyproline are high, and good moisturizing and anti-photoaging effects are achieved.

This invention relates to microorganisms that produce L-glutamic acid and a method for producing amino acids. The method involves culturing microorganisms in a culture medium that have the ability to produce one or more amino acids selected from L-glutamic acid, L-glutamine, L-proline, L-ornithine, L-citrulline, and L-arginine, and that have been modified to increase the activity of α-ketoglutarate synthase, thereby generating and accumulating the L-amino acids in the culture medium or in the cells, and collecting the L-amino acids from the culture medium or in the cells.

The invention belongs to the technical field of gene engineering, and particularly relates to application of an OsACX4 gene in regulation and control of rice salt tolerance. The gene related to the rice salt tolerance is identified for the first time and is named as OsACX4, under treatment of different salt concentrations, the salt tolerance of the OsACX4 gene editing function deletion mutant is remarkably improved, and the growth vigor and survival rate of the OsACX4 gene editing function deletion mutant are higher than those of a wild type and an overexpression strain. After salt treatment, the activity of the OsACX4 deletion mutant and ROS removal related enzymes is generally higher than that of a wild type, the ROS cumulant in vivo is lower than that of the wild type, the content of proline for maintaining cell osmotic equilibrium is higher than that of the wild type, and the content of MDA positively related to the cell membrane damage degree is lower than that of the wild type. The results show that the OsACX4 gene negatively regulates and controls the salt tolerance of the rice in the seedling stage, and is a key gene of the rice in response to salt stress in the seedling stage. The method has important application value in the aspects of analyzing a plant salt-tolerant mechanism and breeding salt-tolerant rice varieties.

The invention belongs to the technical field of walnut protein preparation, and particularly relates to walnut protein powder with high digestibility, low sensitization and low bitterness as well as a preparation method and application of the walnut protein powder. According to the preparation method of the walnut protein powder with high digestibility, low sensitization and low bitterness provided by the invention, proline specific endo protease, recombinant trypsin and carboxypeptidase B are cooperatively used, so that accurate damage to an anti-digestion structure and a sensitization epitope of walnut protein can be realized; according to the method, ultrahigh digestibility (greater than or equal to 92%), thorough desensitization (greater than 99.8%), bitterness inhibition and functional peptide enrichment are synchronously realized, so that the defects of low digestibility, sensitization residue, obvious bitterness, low yield of functional peptide, complex process and the like caused by uncontrollable cutting in the existing random enzymolysis technology are overcome, and meanwhile, the core technical bottleneck of high-value utilization of walnut protein is overcome.

The invention discloses a yak bone collagen peptide for improving osteoporosis and preparation and application thereof, and relates to the field of biological medicine, the yak bone collagen peptide comprises collagen peptide with the molecular weight of 500-2000 Daltons, contains a glycine-proline-hydroxyproline tripeptide core structure, can promote osteoblast differentiation and inhibit osteoclast activity, and can be used for treating osteoporosis. The amino acid sequence of the collagen peptide is any one of the following sequences: glycine-proline-hydroxyproline-glycine-glutamic acid-arginine, and the amino acid sequence of the collagen peptide is any one of the following sequences: glycine-proline-hydroxyproline-glycine-glutamic acid-arginine; and the amino acid is glycine, proline, hydroxyproline, serine, leucine and alanine. The yak bone collagen peptide has a unique glycine-proline-hydroxyproline tripeptide structure, the molecular weight is controlled within the range of 500-2000 Daltons, the structure can promote osteoblast differentiation and inhibit osteoclast activity at the same time, and the problem that a traditional osteoporosis treatment medicine is single in function is solved.

The present application relates to a pyridoxal kinase mutant, a recombinant expression vector and a microbial cell and their applications, and belongs to the technical field of biological catalysis. In order to solve the problem of low product concentration in existing enzyme catalysis, a pyridoxal kinase mutant is provided, the amino acid sequence is selected from the amino acid sequence shown in SEQ ID NO. 1, the lysine at position 229 is mutated to alanine, phenylalanine, methionine, arginine, threonine, histidine, serine, tyrosine, valine, leucine, isoleucine, proline, asparagine, aspartic acid or glutamic acid; the pyridoxal kinase mutant is used for catalyzing pyridoxal to synthesize pyridoxal phosphate, and a recombinant expression vector and a microbial cell can be further formed. The present application has good enzyme activity, high product conversion rate, high concentration of pyridoxal phosphate obtained, and the concentration of the product catalyzed by the wild-type pyridoxal kinase to phosphorylate pyridoxal is obviously improved.

The invention relates to the technical field of crop planting, and provides a method for promoting germination of brassica napus seeds under low-temperature stress by using allantoin. The method comprises the following steps: firstly, disinfecting cabbage type rape seeds, and then soaking the cabbage type rape seeds in an allantoin solution with a specific concentration under room-temperature and weak-light conditions; the treated seeds do not need to be washed and are directly placed in a culture dish paved with double-layer filter paper, a proper amount of distilled water is added, and then the seeds are cultured in an incubator simulating a low-temperature environment. Experimental results show that the germination rate, the germination index and the vitality index of the brassica napus seeds are remarkably improved through allantoin treatment, and the average germination time is shortened. Mechanism research finds that allantoin improves the low-temperature resistance of the brassica napus by various ways of increasing the content of free water of seedlings, increasing the content of soluble sugar and proline, enhancing the activity of antioxidant enzyme, reducing active oxygen and malonaldehyde accumulation and the like. The method disclosed by the invention is low in cost and environment-friendly, and provides powerful guarantee for field production such as late direct seeding of the brassica napus.

The present invention relates to RSV F protein mutants and their applications, belonging to the field of vaccines. Based on the amino acid sequence of wild-type RSV F protein, the present invention has carried out modifications in multiple aspects and in various ways, including disulfide bond mutations, proline mutations, cavity filling mutations, electrostatic mutations, p27 sequence modifications, and / or trimerization domain modifications, and designed a variety of RSV F protein mutants. Then, through gene synthesis, expression, and ELISA detection, the present invention tested the pre-fusion conformation (pre-F) ratio and stability under high and low temperature conditions of the RSV F protein mutants, and screened out several RSV F protein mutants with a relatively high pre-fusion conformation ratio and stable structure. Finally, candidate antigen representatives were selected for immunogenicity detection, which proved that they have good immunogenicity and can be used for the development of RSV vaccines, the preparation of RSV-specific antibodies, or RSV detection reagents.

The invention relates to the technical field of concealed project acceptance, and discloses an alpine region bluegrass cold-resistant growth-promoting nutrient solution and an application method thereof.The nutrient solution is composed of main nutrient elements such as nitrogen, phosphorus and potassium and microelements such as iron, manganese, zinc, copper and boron, and amino acid substances such as glycine, alanine and proline are added; therefore, comprehensive and balanced nutrition support is provided. Through a scientific fertilization strategy, including three stages of seed treatment, seedling stage management and growth stage management, and in combination with a drip irrigation system and a foliage spraying technology, efficient utilization of nutrients and healthy growth of plants are ensured. According to the method, the growth speed and biomass of the bluegrass are remarkably increased, the soil structure is improved, the stress resistance of crops is enhanced, the nutrient utilization efficiency is improved, and finally the product quality and market value are improved; the comprehensive benefits are beneficial to promoting the sustainable development of agriculture in alpine regions.

The invention relates to a green preparation method and application of an anti-saccharification tea extract, and belongs to the technical field of biology. The preparation method of the tea extract comprises the following steps: extracting tea by using a deep eutectic solvent to obtain the tea extract, and carrying out deproteinization and decoloration to obtain the tea extract. The hydrogen bond acceptor of the deep eutectic solvent comprises proline or betaine, and the hydrogen bond donor of the deep eutectic solvent comprises xylitol or glycerol. By utilizing the NaDES extraction technology, effective components can flow out by efficiently breaking cell walls, so that the extraction efficiency of tea active components represented by tea polyphenol is effectively improved; the selected NaDES solvent is natural, green and easy to obtain; the tea extract disclosed by the invention has an anti-saccharification effect, blocks glycosylation reaction, reduces and inhibits generation of AGEs, improves the problems of dark skin, skin laxity and aging caused by saccharification, reduces collagen loss, keeps skin elasticity and delays skin aging.

The invention belongs to the technical field of traditional Chinese medicines and cosmetics, and particularly relates to eutectic resveratrol, a liposome thereof, a preparation method and application of the eutectic resveratrol. The eutectic resveratrol is prepared from the following raw materials: resveratrol, amino acid and glycerol; the amino acid is selected from one of proline, lysine and arginine. The eutecticevaporate resveratrol liposome is prepared from the following raw materials: soybean phospholipid, cholesterol and eutecticevaporate resveratrol, the mass ratio of the soybean phospholipid to the cholesterol is (1-9): 1, and the mass ratio of the sum of the mass of the soybean phospholipid and the cholesterol to the mass of the eutecticevaporate resveratrol is 1: (0.5-2). A method capable of improving the solubility and stability of resveratrol is found, the eutectic resveratrol liposome which is stable in particle size and PDI and excellent in zeta potential and has certain anti-oxidation and antibacterial performance is prepared, the solubility, stability and bioavailability of resveratrol (RES) are improved, and the resveratrol liposome is suitable for being used as an anti-inflammatory drug. And a new thought is provided for further development and application research of resveratrol (RES).

The present disclosure relates to a protein-based article comprising a film having a thickness of about 0.1 μm to about 10 mm and comprising a crosslinked network of partially ordered polypeptides, wherein each partially ordered polypeptide independently comprises a plurality of disordered domains and a plurality of ordered domains, wherein each disordered domain independently comprises a PG or GP motif and each ordered domain independently comprises a polyalanine motif or a polyproline motif.

The invention provides the cassava low-temperature-resistant MeSR45-2 gene cloned from cassava for the first time, the expression of the MeSR45-2 gene in cassava is inhibited under low-temperature stress, and research shows that the gene can significantly improve the low-temperature resistance of cassava, reduce the malondialdehyde content, proline cumulant and the like in cassava, reduce leaf damage under low-temperature treatment and improve the yield of cassava. Terminal buds under low-temperature treatment are not affected and can normally grow, and expression of various protein genes is regulated and controlled. The invention provides a new candidate gene for research on improvement of plant low temperature resistance and the like, and also provides a new gene resource for cultivation of a new variety of low temperature resistant cassava and construction of a new variety of transgenic cassava.