42 results about "Bacillus megatherium" patented technology

The invention discloses a rapid restoration method for degraded grassland based on a microbial agent. The microbial agent is formed by compounding 15-30 parts of a nitrogen-fixing microbial agent, 10-20 parts of a phosphate-solubilizing microbial agent, 8-15 parts of a plant growth-promoting microbial agent, 25-40 parts of a humic acid carrier, 3-8 parts of an embedding agent sodium alginate and 5-12 parts of a nutritional agent molasses. Wherein the nitrogen-fixing bacteria are azotobacter chroococcum and alfalfa rhizobium (the viable count is greater than or equal to 5 * 10 < 9 > CFU / g), the phosphate solubilizing bacteria are bacillus megatherium (the viable count is greater than or equal to 1 * 10 < 10 > CFU / g), and the growth-promoting bacteria are bacillus subtilis and pseudomonas fluorescens (the viable count is greater than or equal to 2 * 10 < 10 > CFU / g). The microbial inoculum is subjected to activation, carrier adsorption, sodium alginate embedding granulation and CaCl2 cross-linking processes to be prepared into slow-release particles of 1-3 mm. 200-250 kg of organic fertilizer is applied to each hectare in a mild area, 300-400 kg of organic fertilizer is applied to each hectare in a moderate and severe area, and leymus chinensis and wheatgrass are sown in a mixed mode. After 90 days of application, the vegetation coverage is improved by 40% or above, the soil organic matter is increased by 25-40%, the microbial diversity index is larger than or equal to 3.5, and the comprehensive remediation cost is reduced by 53%.

The invention provides bacillus megaterium and application thereof in preparation of a nano-selenium organic fertilizer and improvement of the selenium content of tea leaves, and belongs to the technical field of microorganisms, the bacillus megaterium NT2 is preserved in the China Center for Type Culture Collection with the preservation number of CCTCC NO: M 20251806, and the bacillus megaterium NT2 is preserved in the China Center for Type Culture Collection with the preservation number of CCTCC NO: M 20251806. The bacillus megatherium NT2 has relatively strong resistance to reduction on selenium element, can survive in high-concentration inorganic selenium, can reduce the inorganic selenium into nano-selenium, and has relatively weak capability of enriching arsenic, cadmium and lead; the selenium source of the nano-selenium organic fertilizer can be prepared by mixed fermentation with high-selenium rock powder which is calcined and activated at high temperature, so that the content of heavy metals does not exceed the national standard while the selenium content of the tea leaves is increased. The bacillus megaterium provided by the invention not only can reduce the toxicity of inorganic selenium in high-selenium rock and convert the inorganic selenium into nano-selenium, but also can stably, safely and standardly produce selenium-rich tea leaves, the heavy metal content of the tea leaves does not exceed the standard, and the bacillus megaterium has a wide market prospect.

The invention discloses application of bacillus megaterium and a biological fertilizer thereof in rice planting, and relates to the technical field of microorganisms. The bacillus megaterium is preserved in the China General Microbiological Culture Collection Center on July 10, 2025, and the preservation number of the bacillus megaterium is CGMCC (China General Microbiological Culture Collection Center) NO.35179. The bacillus megaterium has the advantages that the bacillus megaterium is preserved in the China General Microbiological Culture Collection Center on July 10, 2025; the strain is a plant growth-promoting bacterium and has a good rice growth-promoting effect, and when the prepared biological fertilizer is applied to the rice planting process, compared with a control group, the tiller number of rice is increased by 64.44%, the plant height is increased by 2.77%, and the leaf chlorophyll content is increased by 8.38%. The bacillus megatherium provided by the invention and the biological fertilizer prepared by strain fermentation have the advantages of greenness, safety, high efficiency and the like, and have a better application prospect in the aspect of agricultural production.

The invention discloses an acid soil conditioner containing microalgae, and belongs to the technical field of agriculture and environmental restoration. The acid soil conditioner containing the microalgae is prepared from the following components in parts by weight: 100 to 130 parts of biomass charcoal, 30 to 40 parts of vermiculite, 1 to 2 parts of brachyristichum brachyristichum, 0.1 to 0.5 part of a bacillus mucilaginosus microbial agent, 0.1 to 0.5 part of a bacillus subtilis microbial agent and 0.1 to 0.5 part of a bacillus megatherium microbial agent. According to the method disclosed by the invention, the brachyristichum brachyristichum which can be planted under an acidic condition and can generate alkaline metabolites to increase the pH value of acidic soil is selected as a main functional component, and is matched with other components with functions of loosening soil, helping the brachyristichum brachyristichum to proliferate, providing organic matters, helping the soil to release nutrients and the like; and compounding to obtain the soil conditioner capable of effectively improving acid soil.

The invention provides bacillus megatherium for preventing and treating soil-borne diseases of crops, a microbial inoculum and application of the bacillus megatherium and the microbial inoculum. The bacillus megaterium HN-2 disclosed by the invention is classified and named as bacillus megaterium, the bacillus megaterium HN-2 is preserved in the general microbiological center of the China Committee for Culture Collection of Microorganisms, and the preservation number of the bacillus megaterium HN-2 is CGMCC No.34657. A test result shows that the antibacterial effect of the bacillus megaterium HN-2 on four kinds of fusarium and tobacco black shank bacteria reaches 60.98%-71.12%; the indoor nematode killing effect on wheat cyst nematode and tomato root-knot nematode reaches 100%. The optimized binder, wetting agent, stabilizing agent and disintegrating agent are respectively starch, lauryl sodium sulfate, CaCO3 and AlCl3; after seed dressing and broadcasting, the field cyst decline rates of wheat cyst nematode and soybean cyst nematode are 60.22% and 52.58% respectively, the field prevention effects of wheat root rot and soybean root rot are 69.50% and 54.44% respectively, and the biocontrol inoculant has wide application potential.

The invention relates to the field of acid soil conditioners, in particular to a charcoal-based acid soil conditioner as well as a preparation method and application thereof. The biochar-based acid soil conditioner comprises a biochar carrier and a complex microbial inoculant loaded on the biochar carrier, and the complex microbial inoculant comprises 25-35 parts of nitrogen-fixing functional bacteria; 20 to 40 parts of phosphorus and potassium solubilizing bacteria; 15 to 25 parts of nitrogen activating bacteria; 15 to 25 parts of synergistic growth promoting bacteria; the nitrogen-fixing functional bacteria comprise Azospirillum brasilense and / or Klebsiella, the phosphate-solubilizing and potassium-solubilizing bacteria comprise bacillus megatherium and / or bacillus mucilaginosus, the nitrogen-activating bacteria comprise urease-producing bacillus and / or nitrospirillum, and the synergistic growth-promoting bacteria comprise arbuscular mycorrhizal fungi and / or pseudomonas. The complex microbial inoculant is constructed by screening four functional strains of nitrogen fixation, phosphorus and potassium solubilization, nitrogen activation and synergistic growth promotion, a complete functional closed loop of nitrogen fixation, phosphorus solubilization, nutrient activation and crop growth promotion is formed, and synergistic interaction is achieved through functional complementation of the strains.

This invention belongs to the field of bio-mineral fertilizer technology, specifically relating to a microbial liquid compound agent and bio-mineral fertilizer that combine coal gangue activation and heavy metal remediation. The microbial liquid compound agent comprises the following functional strains prepared through three-stage sequential inoculation and fermentation using electrochemical low-Hertz water: *Pseudomonas putida* (a remedial bacterium), *Azotobacter chrysogenum* (a nitrogen-fixing bacterium), *Azospirillum brasiliensis* (a growth-promoting bacterium), *Pseudomonas fluorescens* (a phosphate-solubilizing bacterium), *Bacillus subtilis* (a biocontrol bacterium), *Bacillus mucilaginosus* (a potassium-solubilizing bacterium), *Bacillus megaterium* (a silica-solubilizing bacterium), and *Trichoderma echinococcus* (a synergistic bacterium). The bio-mineral fertilizer is prepared using the aforementioned microbial liquid compound agent, with coal gangue powder as the main raw material. The scientific combination of different strains provided by this invention achieves efficient improvement of heavy metal-contaminated land and increased crop yields. Simultaneously, it transforms coal gangue into a valuable resource, solving the pollution problem caused by the large-scale accumulation of coal gangue, and possesses significant economic and social value.

This invention relates to a novel Bacillus megatherium HyangYak-01 strain and its applications. According to one embodiment of the novel Bacillus megatherium HyangYak-01 strain, it exhibits effects such as increasing the diversity of microbiome species in soils used for crop cultivation or improving their physicochemical properties, and promoting crop growth or the biosynthesis of physiologically active substances. Therefore, it can be applied to a variety of uses, such as improving soil conditions, increasing crop cultivation efficiency, or as a fertilizer to enhance crop efficacy.

The application discloses a high-phosphorus hematite biological desphosphorization method based on micro-electric field stepwise domestication and nutrient slow-release regulation, and belongs to the technical field of mineral processing and bio-metallurgy. The biological desphosphorization method comprises the following steps: culturing Bacillus subtilis and Bacillus megaterium to the logarithmic growth phase to obtain active bacteria suspension of a combined bacterial flora; placing the active bacteria suspension in different high-phosphorus hematite slurries from low concentration to high concentration, and obtaining a dominant functional bacterial flora under the regulation of micro-electric field and nutrient slow release; culturing the dominant functional bacterial flora to the logarithmic phase, then adding pretreated high-phosphorus hematite powder for leaching, monitoring the pH value, and separating to obtain leaching solution I and leaching residue I; mixing the leaching residue I with a fungal leaching medium, inoculating a fungus Talaromyces for synergistic leaching, and separating to obtain leaching solution II and desphosphorized iron concentrate. The application successfully constructs an efficient, stable and environment-friendly functional bacterial flora + fungus synergistic system, and realizes efficient removal of phosphorus elements in high-phosphorus iron ore in a short period.

Disclosed in this disclosure are Acid Sulfate Soil (ASS) improvement materials, and preparation methods and application methods thereof. ASS improvement materials include mixed particle improvement materials and microbial improvement materials. The mixed particle improvement materials include natural mineral materials, organic carbon materials, lignosulfonates, silicates, high molecular polymers, shell powder, fish bone powder, and hydrated lime powder; and the microbial improvement materials include Bacillus megaterium, Bacillus subtilis, Bacillus licheniformis, Bacillus amyloliquefaciens, Trichoderma harzianum, yeast, and photosynthetic bacteria.During use, the mixed particle improvement material is evenly distributed in the soil, and is well mixed with the soil through rotary tillage; the microbial improvement material is dissolved in water and added to the irrigated land, the water surface depth is 5-10 cm, the land is continuously soaked for 5-7 days, and then agricultural cultivation activities are carried out after the water level naturally decreases. The ASS improvement material in the present disclosure has the effects of deacidification, maintenance and improvement of fertilizer, and increase in production, and has the characteristics of efficiency, green, environmental friendliness, etc.

This application provides a screening method and animal model related to intraocular diseases, such as age-related macular degeneration (AMD), for identifying candidate therapeutic agents for treating or preventing eye diseases, such as AMD. [Solution] The present application also provides compounds / compositions that can kill or inhibit the growth of microorganisms such as Bacillus megatherium. The present application further provides methods for treating infections caused by microorganisms such as Bacillus megatherium, and for treating or preventing diseases or disorders associated with such infections, such as AMD, using the compounds / compositions.

The invention discloses a microorganism for treating moss and foul smell in park landscape lake water and a culture process thereof, the microorganism comprises biphase photosynthetic bacteria, decomposing bacteria and a culture medium, the decomposing bacteria comprise bifidobacterium, mesophilic actinomycetes, nitrosobacteria and bacillus megatherium, the weight percentage of the decomposing bacteria is 1-5%, and the weight percentage of the decomposing bacteria is 1-5%. The microbial inoculant comprises the following components in percentage by weight: 20-30% of biphasic photosynthetic bacteria, 15-25% of bifidobacterium, 10-20% of mesophilic actinomycetes, 10-20% of nitrosobacteria and 15-25% of bacillus megatherium. The biphase photosynthetic bacteria and the composite decomposition bacteria are scientifically proportioned to form a microbial system with complementary functions, the biphase photosynthetic bacteria can directionally inhibit photosynthesis of moss, destroy cell structures of moss and realize targeted killing, and the decomposition bacteria synergistically decompose organic matters such as underwater residual feed, excrement, animal and plant bodies and the like, so that the biological activity of moss is improved. Meanwhile, nitrosobacteria can convert algal toxins into harmless substances, the synergistic effect overcomes the defects that an existing single microbial preparation is single in function and incomplete in treatment, and integrated treatment of killing moss, eliminating stink and improving water quality is achieved.

The present application relates to the technical field of bio-organic fertilizer preparation, and particularly relates to a bio-organic fertilizer prepared by microbial fermentation and a preparation method thereof, which is prepared by fermentation of raw materials and compound microbial strains, wherein the raw materials include livestock and poultry manure, crop straw, industrial organic waste residue and a humification agent, and the compound microbial strains are a ternary combination of bacillus subtilis, bacillus mycoides and saccharomyces cerevisiae, or a quaternary combination of bacillus subtilis, bacillus mycoides, saccharomyces cerevisiae and bacillus mucilaginosus. The present application adopts a multi-element compound strain combination and optimizes the ratio, and the synergistic effect of each strain is significant, which greatly improves the organic matter degradation efficiency and effective nutrient activation capacity, and compared with single strain, the microbial activity is stronger, and the nutrient supply capacity of the fertilizer is better.

The invention relates to the technical field of soil remediation, in particular to a saline-alkali soil remediation fungicide and a preparation method thereof. Comprising the following raw materials in parts by weight: 4 to 8 parts of bacillus subtilis, 8 to 15 parts of purple sulfur bacteria, 10 to 17 parts of rhizobium, 1 to 3 parts of bacillus megatherium, 1 to 3 parts of candida tropicalis, 1 to 3 parts of aspergillus niger, 0.05 to 0.2 part of proline, 0.5 to 2.5 parts of humic acid, 3 to 5 parts of modified charcoal, 0.1 to 0.5 part of glucose, 0.4 to 1.2 parts of monopotassium phosphate and 0.3 to 0.6 part of calcium chloride. The application of the soil remediation fungicide not only improves the growth environment of the crops, but also obviously improves the salt tolerance of the crops by adjusting the stress resistance mechanism of the crops. After the soil remediation fungicide is applied, the root system development of crops is more robust, and the density and length of the root system are obviously increased, so that the crops can absorb water and nutrients from the soil deeper layer, and the surface salt damage can be effectively resisted.

The invention relates to a microbial agent with a deodorization effect as well as a preparation method and application of the microbial agent. The bacterial strains in the microbial agent comprise a bacillus megaterium YBG02 bacterial strain with the preservation number of CCTCC (China Center for Type Culture Collection) NO: M2021052 and a bacillus subtilis YBS01 bacterial strain with the preservation number of CCTCC NO: M2021053. The bacillus megaterium and the bacillus subtilis both belong to strains safe to human, animals and the environment and have an obvious odor removal effect, and the prepared microbial inoculum can be widely applied to cat litter production and has the advantages of being good in temperature resistance and low in loss rate.

The invention relates to the technical field of flower planting, in particular to a beneficial bacterium nutrient solution for promoting orchid growth and a preparation method and application thereof. The beneficial bacterium nutrient solution disclosed by the invention is prepared from the following components in parts by mass: 0.02 to 0.05 part of a bacillus megaterium bacterium agent, 0.02 to 0.06 part of a bacillus amyloliquefaciens bacterium agent, 0.3 to 0.8 part of bacillus coagulans, 0.1 to 0.5 part of a bacillus subtilis bacterium agent, 0.04 to 0.08 part of a lactobacillus plantarum bacterium agent, 0.5 to 1.0 part of trichoderma harzianum, 2 to 8 parts of humic acid, 1 to 5 parts of potassium fulvate, 2 to 8 parts of sodium alginate, 4 to 10 parts of monopotassium phosphate and 1800 to 2000 parts of waste liquid. The beneficial bacterium nutrient solution prepared from the components can promote growth of orchid roots, increase the number of orchid flowering branches and prolong the flowering period of orchid.

The invention relates to the field of microbial agents, in particular to a micro-ecological restoration liquid composite microbial agent suitable for saline-alkali soil and a biological bacterial fertilizer of the micro-ecological restoration liquid composite microbial agent. The micro-ecological restoration liquid complex microbial inoculant suitable for the saline-alkali soil is prepared from restoration salt-tolerant bacteria, namely bacillus subtilis, nocardia halospora, nitrogen-fixing bacteria, namely azotobacter chroococcum, phosphate solubilizing bacteria, namely pseudomonas fluorescens, potassium solubilizing bacteria, namely bacillus mucilaginosus, growth-promoting bacteria, namely azospirillum brasilense, biocontrol bacteria, namely bacillus megatherium and synergistic bacteria, wherein the restoration salt-tolerant bacteria are bacillus subtilis, nocardia halospora, the nitrogen-fixing bacteria are azotobacter chroococcum, the phosphate solubilizing bacteria are pseudomonas fluorescens, the potassium solubilizing bacteria are bacillus mucilaginosus, the growth-promoting bacteria are azospirillum brasilense, the biocontrol bacteria are bacillus megatherium, and the synergistic bacteria are prepared from bacillus subtilis The biological bacterial fertilizer for the saline-alkali soil is prepared from the liquid complex microbial inoculant, compared with a conventional biological bacterial fertilizer, the adaptability to the saline-alkali soil is greatly improved, the saline-alkali soil is effectively improved, and the crop yield is remarkably increased.

The application relates to the technical field of biological fertilizer, and particularly discloses a compound microbial fertilizer and a preparation method thereof. The Klebsiella pneumoniae, Bacillus megaterium, Bacillus mycoides and Bacillus subtilis are compounded, the Klebsiella pneumoniae converts nitrogen in the air into nitrogen available for plants, the Bacillus megaterium decomposes insoluble phosphorus in soil into effective phosphorus, the Bacillus mycoides decomposes potassium silicate minerals in soil to release effective potassium, the Bacillus subtilis produces biological substances to stimulate root growth and secretes antibacterial substances to inhibit pathogenic bacteria in roots. The modified biochar carrier is used to provide stable living space for the compound microbial fertilizer, so that the compound microbial fertilizer is sustainable, efficient and green.

The invention discloses a microorganism for treating cyanobacteria and stink in park landscape lake water and a culture process thereof, the microorganism comprises a microbial agent, a compound enzyme, tartaric acid and free fatty acid, and the microbial agent is composed of biphasic photosynthetic bacteria, bifidobacterium, mesophilic actinomycetes, nitrosobacteria and bacillus megatherium. The microbial agent is compounded with the compound enzyme, the tartaric acid and the free fatty acid, five strains in the microbial agent are clear in division of labor, the biphasic photosynthetic bacteria decompose organic matters released by cyanobacteria residues and reduce ammonia nitrogen, the bifidobacterium adjusts the balance of water flora and inhibits reproduction of malodorous bacteria, and the mesophilic actinomycetes secretes antibacterial substances to prevent recurrence of the cyanobacteria. Nitrosobacteria convert an odor source into harmless substances, bacillus megaterium further secretes extracellular enzyme to accelerate degradation of residues, and microorganisms and bioactive substances have a synergistic effect, so that the removal period of blue-green algae is shortened, and the odor removal rate is increased and is far superior to that of a single microbial preparation.

The invention discloses a water-preserved mining-oriented underground water plugging material and an underground water plugging method, and relates to the technical field of water-preserved mining, the underground water plugging material comprises calcium sulphoaluminate cement clinker, micro-nano Fe3O4, water glass, bacillus megatherium powder, urea, anhydrous CaCl2 powder and azodicarbonamide, and the plugging reliability can be improved by adopting the water-preserved mining-oriented underground water plugging material and the water-preserved mining-oriented underground water plugging method.

The invention discloses preparation of an immobilized material based on phosphate solubilizing bacteria and application of the immobilized material in soil, and belongs to the technical field of soil remediation microbial agents. The material takes bacillus megatherium and lignin as core functional components, and takes a mixed system of sodium alginate and sodium carboxymethyl cellulose as a carrier. The immobilized material is high in mechanical strength, good in stability and excellent in mass transfer efficiency, meanwhile, insoluble phosphorus is activated through metabolites secreted by bacillus megatherium, and the content of available phosphorus in soil is increased. The material can also improve the soil structure, improve the cation exchange capacity and the organic matter content, enhance the activities of peroxidase and superoxide dismutase of the rice, reduce the content of malonaldehyde, relieve oxidative damage caused by Cd stress, reduce Cd accumulation of the rice from the source and guarantee normal growth of the rice.

The invention discloses a monooxygenase mutant capable of generating (4S)-4-hydroxyisophorone and a preparation method of the monooxygenase mutant. The monooxygenase is cytochrome P450BM3 monooxygenase from bacillus megatherium. The cytochrome P450BM3 monooxygenase mutant has high stereoselectivity and high reaction activity for catalyzing hydroxylation of isophorone, the diastereomer win rate of the (4S)-4-hydroxy isophorone obtained by catalyzing isophorone through the mutant R47L / Y51F / F81W / A191T / N239H / I259V / A276T / A328I / A330S / L353 I / I401L / L437LL can reach 95%, and the yield can reach 96%; the process for catalyzing isophorone to synthesize (4S)-4-hydroxy isophorone by using the cytochrome P450BM3 monooxygenase mutant is simple and environment-friendly, and has a good industrial prospect.

The invention relates to the field of microorganisms, in particular to a strain composition, a microbial agent and application thereof. The invention provides a bacterial strain which comprises a bacterial strain 1 or a bacterial strain 2; the bacterial strain 1 is P.megaterium, and the preservation number of the bacterial strain 1 is CGMCC (China General Microbiological Culture Collection Center) No.36177; the bacterial strain 2 is bacillus megatherium, and the preservation number of the bacterial strain 2 is CGMCC (China General Microbiological Culture Collection Center) No.36179. By screening efficient multifunctional strains, the effect stability and functional diversity of the microbial agent are remarkably improved, and the bottleneck that existing strain resources are limited is broken through. The developed salt-tolerant plant growth-promoting microbial agent not only has good salt-tolerant characteristic, but also can effectively promote plant growth, and has important application value and popularization prospect in the aspects of improving the stress resistance of crops and improving the ecological environment of salinized soil.

The present application relates to a kind of bacterial agent with deodorization effect and its preparation method and application, the strain in the bacterial agent includes the Bacillus megaterium YBG02 strain with the preservation number CCTCC NO:M2021052 and the Bacillus subtilis YBS01 strain with the preservation number CCTCC NO:M2021053.The Bacillus megaterium and Bacillus subtilis used in the present application are all safe strains to human, animal, environment, have obvious removal effect on odor, and the bacterial agent prepared can be widely applied in cat litter production, with good temperature resistance and low loss rate.

The invention belongs to the technical field of new materials, pollution treatment and microorganisms, and particularly relates to an iron mineral-microorganism biological membrane, a preparation method thereof and application of the iron mineral-microorganism biological membrane in degradation of pollutants in a dark environment. The iron mineral-microbial biological membrane provided by the invention comprises a lamellar biological membrane formed by bacillus megatherium and iron minerals dispersed in the biological membrane. The iron mineral-microbial biological membrane converts photo-induced electrons into stable chemical energy to be stored, electrons are continuously released in a dark environment, and efficient degradation of pollutants in the dark environment is achieved.

This invention belongs to the field of fertilizer technology, specifically relating to a method for preparing a special fertilizer for saline-alkali land using agricultural waste. The method involves using *Pseudomonas halophilus*... Halopseudomonas salina With Bacillus subtilis Bacillus subtilis With Bacillus megaterium Bacillus megaterium A compound fermented seed liquid was prepared by mixing seed liquids at a mass ratio of 1-3:1-3:1-3. Rice straw, corn straw, peanut shells, and livestock manure were used as fermentation substrates, mixed with water, and inoculated with the compound fermented seed liquid. Fermentation was carried out at 30-32℃ for 9-12 days to obtain bio-fertilizer. The bio-fertilizer was then mixed with humic acid and trace element fertilizers, granulated, and coated with polylactic acid to produce a slow-release fertilizer. This fertilizer can effectively reduce the pH and salinity of saline-alkali soils, increase organic matter content, and promote crop growth.

The application discloses a kind of generation (4R) 4-hydroxyisophorone monooxygenase mutant and its preparation method, the monooxygenase is derived from cytochrome P450 of bacillus megaterium BM3 Monooxygenase. The cytochrome P450 BM3 Monooxygenase mutant has high stereoselectivity and high reactivity to catalyze isophorone hydroxylation, mutant A74G / V78A / F87V / L188Q / T327A / A328F catalyzes isophorone to obtain (4R) The diastereomeric excess of 4-hydroxyisophorone can reach 99%, and the yield can reach 98%; the cytochrome P450 BM3 Monooxygenase mutant catalyzes isophorone synthesis (4R) 4-hydroxyisophorone production process is simple, reaction condition is mild, production process is environmental protection, is conducive to industrial production, and has wide application prospect.

The application provides a microbial agent for decomposing pollutants and a preparation method and application thereof, and the microbial agent for decomposing pollutants comprises the following raw materials in parts by weight: 40-80 parts of compound bacteria, 15-25 parts of enzyme preparation, 10-15 parts of growth substrate, 3-6 parts of nutrient element, 1-3 parts of stress resistance regulator, 0-0.15 part of azido-erythro-sphingosine, and 0-0.05 part of polyvinyl caprolactam; wherein the compound bacteria are one or more of Bacillus megaterium, Bacillus subtilis, Bacillus pumilus, Pseudomonas fluorescens, Bacillus licheniformis and Bifidobacterium pseudolongum; and the stress resistance regulator is a mixture of ethoxylated castor oil and dodecyl hydroxyl poly (oxy-1,2-ethanediyl). The microbial agent for decomposing pollutants has less influence on salt components and toxic substances in wastewater, can still achieve high decomposition efficiency in an environment with high salt content and toxic substances, and has good treatment effect on petrochemical wastewater.

The application discloses a method for improving tannin of wine grape by using bacillus megaterium, and belongs to the technical field of plant cultivation. The method comprises the following steps: (1) inoculating bacillus megaterium strains into a culture solution, carrying out fermentation culture, and obtaining a bacterial liquid; (2) mixing the bacterial liquid with a nutrient solution, and obtaining a bacterial agent; (3) respectively applying the bacterial agent to root irrigation in a germination period and an initial fruiting period of the wine grape, and performing foliar spraying in a color changing period; and the bacillus megaterium strains are composed of bacillus megaterium CGMCC 1.8788 and bacillus megaterium CGMCC 1.8569. The bacterial agent prepared by using the bacillus megaterium is applied in different periods of wine grape growth, can promote the accumulation of tannin in the wine grape, improves the quality of the wine grape, provides a reference for a new application of the bacillus megaterium, and provides an experience method for the cultivation of the wine grape.

The invention provides a microbial agent for efficiently degrading kitchen waste. The microbial agent comprises a specific strain and a carrier. According to the microbial agent, efficient degradation of kitchen waste is achieved through the synergistic effect of a traditional strain and a thermophilic strain on a carrier; wherein the traditional strains comprise brevibacterium aureum, bacillus licheniformis, bacillus subtilis, bacillus amyloliquefaciens, vegetable bacillus, bacillus tequilensis, stenotrophomonas maltophilia, candida glabrata and pediococcus acidilactici; the thermophilic strains comprise novel bacillus thermophilus, bacillus pumilus, bacillus thermophilus deamination bacillus and bacillus megatherium. The microbial agent is stable in degradation effect, simple and convenient in preparation process, low in cost and free of environmental pollution.