562 results about "Bacillus megatherium" patented technology

The invention discloses complex microbial inoculant which comprises the following active ingredients: bacillus subtilis, bacillus megatherium, trichoderma koningii, aspergillus oryzae, trichoderma viride, geotrichum candidum, aspergillus niger, rhizopus oligosporus, lactobacillus plantarum CICC20768 and azotobacter chroococcum. Correspondingly, the invention further discloses a method for preparing an organic fertilizer from the complex microbial inoculant. The method comprises the following steps: a. uniformly mixing the complex microbial inoculant with a plant fiber raw material to obtain a fermented raw material; b, composting and fermenting the fermentation raw material; c, drying the composted fermentation raw material to obtain the organic fertilizer. microbes of the complex microbial inoculant have no antagonism, and can effectively cooperate in the production process of the organic fertilizer, and the chemical components of the production raw materials can be sufficiently utilized to finally produce the high-quality organic fertilizer. The produced organic fertilizer reaches the parameter indexes of the standard (NY884-2012) of China, can improve the planting property of soil, is beneficial to optimization of soil microbial flora, and can provide comprehensive nutrients to a plant root system.

The invention relates to a preparation and the preparation method for restoring the microecological bacteria on the interface layer of water-sediment. The microecological bacteria preparation uses 0.2-0.4mm of medical stone as carrier, absorbs and fixes the composite microbe optimally combined by bacillus subtilis, bacillus licheniformis, bacillus megatherium, and thin yellow streptomyces. The amount ratio of bacillus subtilis to bacillus licheniformis to bacillus megatherium approximates to one to one to one; the volume ratio of the mixture liquid of the three kinds of bacillus to the thin yellow streptomyces liquid is two to one. The invention has the advantages that spreading the preparation of the invention into the interface layer of functional water body water-sediment in the spring March to April each year can prevent blue algae (aerugo micro-capsule algae is the most) from fulminantly growing in summer June to August. The preparation can improve the quality of water, can prevent eutrophication development, and can promote the carbon, nitrogen, and oxygen circulation on the interface layer of water-sediment. The microecological bacteria preparation has high effective colony amount and strong adaptability; and is effective to restore the microecological environment at the interface layer of water-sediment.

The invention discloses bacillus megatherium, a method for preparing a microbial inoculum through solid fermentation of the bacillus megatherium and an application of the microbial inoculum, belonging to the technical field of microbial preparations. The bacillus megatherium JSSW-JD-2F5 has been preserved in the China General Microbiological Culture Collection Center with the preservation number of CGMCC NO.8045. By adopting solid fermentation, a preparation method of a bacillus megatherium JSSW-JD-2F5 solid fermentation microbial inoculum and the application of the solid fermentation microbial inoculum are disclosed, wherein the solid fermentation microbial inoculum is used as an additive of a livestock, poultry and fishery cultivation feed and is added into the feed according to a mass percentage of 0.01-0.05%. The product of the microbial inoculum contains high-concentration viable bacteria, active enzyme and physiological active matters, and the concentration of the viable bacteria reaches more than 1.0*10<10>CFU/g and can meet requirements of a feeding microbial additive on the viable count; the application of the inoculum not only can improve the breeding environment, but also has an important significance for increasing the feed utilization ratio and promoting the growth of animals, so that the inoculum is widely applied to livestock, poultry and fishery cultivation.

The invention relates to a biological Increasing the quality agent of tobacco and its preparation method and application. the Increasing the quality agent comprises (by weight parts) Bacillus megaterium ZXB1 (CGMCC 1.941) 180-200, Bacillus subtilis subsp. Subtilis (CGMCC 1.354) 180-200, diastase 5-15, amylase 50-200, and protease 450-550. The invention has the advantages of improved product fermentation quality, shortened aging period by 10-12 months, improved chemical ingredients in tobacco, improved tobacco flavor, reduced cost by 3.5 yuan for each box, and improved tobacco grade by one third.

The invention relates to a liquid microbial fertilizer with nitrogen-fixing, phosphorus-dissolving and potassium-dissolving effects, and a preparation method thereof. The liquid microbial fertilizer consists of the following microbial fermentation liquids in parts by volume: 1 to 10 parts of azotobacter chroococcum, 1 to 30 parts of bacillus mucilaginosus, 1 to 10 parts of phosphorus-dissolving bacillus megatherium, 1 to 10 parts of bacillus amyloliquefaciens, 1 to 10 parts of bacillus subtilis, 1 to 5 parts of photosynthetic bacteria and 1 to 10 parts of streptomyces microflavus. The functional bacteria serve as main functional flora of the microbial fertilizer, so that the obtained product has remarkable effects of fixing nitrogen, dissolving phosphorus, dissolving potassium and resisting disease, can enhance the total metabolic activity of soil bacterial community and the diversity of the bacterial community, improve the soil micro-ecological environment, promote growth and reproduction of beneficial microorganisms and inhibit the growth of pathogenic bacteria, is an ideal fertilizer in green agriculture and organic agriculture, and has a wide prospect in agricultural sustainable development.

The invention relates to a phosphorus and potassium decomposing biological organic fertilizer prepared from bacillus megatherium and Bacillus mucilaginosus. The fertilizer comprises the following components in percentage by weight: 30-40% of animal dung, 20-30% of kitchen waste, 15-25% of humic acid, 15-20% of crop straw, 1-2% of microelements and 4-6% of culture. A preparation method of the fertilizer comprises the following steps of: 1, inoculating bacillus megatherium to a nutritional gravy culture medium and the bacillus mucilaginosus to a silicate bacteria culture medium, and spraying a seed liquid after primary and secondary cultures onto a carrier to prepare conidial powder; 2, uniformly mixing 30-40% of animal dung, 20-30% of kitchen waste, 15-25% of turf, 15-20% of straw powder, 1% of potassium dihydrogen phosphate, 1% of zeolite powder, 1% of lime, 3-5% of boric sludge and 1% of gypsum; adjusting water content to 50-60% and stacking the materials into a fermentation tank; adding enzyme microorganisms and turning over mechanically; and fermenting for 3-4 weeks and drying, and crushing to obtain a finished product and packaging. According to the biological organic fertilizer, not only is the soil capacity enhanced, but also nutritional elements which can be absorbed and utilized are provided for crops.

The invention relates to a compound microbial fertilizer and a preparation method thereof. The compound microbial fertilizer is characterized by being prepared from the following materials in percentage by weight: 40.0-50.0 % of rape seed cakes, 10.0-20.0% of crops straws, 1.5-3.0% of mycothallus, 0.5-1.0% of bacterium thallus and 35.0-40.0% of water. By using the solid fermentation method twice, the invention organically combines the fungi (Trichoderma harzianum and Aspergillus aculeatus for biological control effect) and the bacteria (Bacillus subtillis and Bacillus megatherium for growth-promoting effect), and realizes a simple preparation method. The compound microbial fertilizer has the advantages of environmental condition resistance, high count of active viable bacteria, less influence by the environment after application, slow release of thallus and long-term effective colonization at rhizosphere of plants, promotes plant growth and simultaneously has a certain biological control effect on soil-borne diseases.

The invention provides a composite active bacterial preparation which is used for aquatic products and has the water improving and fertilizing functions and a preparation method thereof by applying a modern bioengineering technology mainly for the problem that in existing aquaculture, the water quality of a pond is poor. The composite microecological preparation is prepared by mixing bacillus subtilis, bacillus megatherium, bacillus amyloliquefaciens, bacillus licheniformis, enterococcus faecium, lactobacillus brevis, lactobacillus plantarum, lactococcus lactis and saccharomyces cerevisiae through liquid-submerged fermentation. In the liquid preparation, microorganisms are in a dormant state and are stable and easy to preserve, after the preparation is put into water, the dormant strains can quickly resuscitate, harmful substances such as nitrite nitrogen and ammonia nitrogen in the water are quickly decomposed, good algae growth is facilitated, harmful algae reproduction is inhibited, and therefore the purpose of improving the water quality of the culture water is achieved.

The invention discloses an environmentally-friendly efficient microbial compound fertilizer and a preparation method thereof. The environmentally-friendly efficient microbial compound fertilizer is prepared from chicken manure, cow dung, dried pond sludge powder, ground phosphate rock, edible fungi residues, furfural residues, a microbial agent, wheat bran, bean cakes, urea, ammonium nitrate, potassium humate, wheat straws, charcoal powder and traditional Chinese medicine residues, wherein the microbial agent is prepared from bacillus licheniformis, bacillus subtilis, bacillus mucilaginosus, bacillus megatherium, pseudomonas, bacillus thuringiensis, bacillus amyloliquefaciens and nitrogen-fixing bacteria. The edible fungi residues and the furfural residues are smashed and mixed with water, the microbial agent is added for fermentation, the Chinese traditional medicine residues are added to perform fermentation, then the rest of the raw materials are added, and the microbial compound fertilizer can be obtained by performing full mixing, granulation and drying. The raw materials used for the microbial compound fertilizer are all natural fertilizers and contain rich mineral substances, the damage of a soil acid-base structure and a nutritional ingredient structure caused by inorganic fertilizer application is avoided, and the environmentally-friendly efficient microbial compound fertilizer is greener and more environmentally-friendly.

The invention discloses a biomass charcoal soil conditioner. The soil conditioner comprises the following raw materials by weight: 40% to 50% of biomass charcoal, 20% to 30% of a functional microbial agent, 10% to 15% of desulfurized gypsum, 5% to 10% of calcium nitrate, 5% to 10% of ground phosphate rock and 1% to 2% of amino acid, wherein the functional microbial agent contains any two selected from the group consisting of bacillus subtilis, bacillus mucilaginosus, bacillus amyloliquefaciens, azotobacter, rhizobia and bacillus megatherium. The biomass charcoal soil conditioner provided by the invention can increase the water-retention rate and air permeability of soil, improve the content of organic matters in the soil, and regulate the pH value of the soil; meanwhile, the adoption of biological means to improve microbial environment of the soil is beneficial for promoting crop growth and high-efficiency nutrient utilization, increases the yield of crops, and improves the quality of the crops. The biomass charcoal soil conditioner provided by the invention effectively utilizes wastes, can improve the soil and enhance soil fertility, and specifically has significant improvement effect on saline alkali land.

The invention relates to a bacillus megatherium SJ-7 strain and an application thereof, and belongs to the technical field of organisms. Strain separation, purification and cultivation are carried out from the natural world by a common method, so as to obtain the bacillus megatherium (bacillus megatherium) SJ-7 strain; the bacillus megatherium SJ-7 strain is reserved in the Common Microorganism Center of the China Committee for Culture Collection of Microorganisms; the reservation number is CGMCC No.7169; and the bacillus megatherium SJ-7 strain has strong effects of solubilizing phosphate and potassium and fixing nitrogen, and has good antagonism on a plurality of disease funguses, and can be applied to preparation of a drug for preventing and treating plant fungi diseases. Compared with the reported other bacillus megatherium, the bacillus megatherium SJ-7 disclosed by the invention has the advantages that the bacillus megatherium SJ-7 has good effects of solubilizing potassium, degrading refractory inorganic phosphorus in soil, and fixing nitrogen, can promote plant growth, and has strong inhibitory action on a plurality of plant pathogenic fungi of causing a gibberellic disease, a cotton drooping disease, wheat sharp eyespot and botrytis.

The invention is suitable for the technical field of recycling organic wastes, and discloses a flora for recycling treatment of organic wastes. The flora for recycling treatment of organic wastes is efficiently compounded from a variety of bacteria such as bacillus subtilis, aspergillus niger, saccharomycete and soil flora, wherein the soil flora is extracted from the natural world and comprises three or more of bacillus megatherium, bacillus mucilaginosus, bacillus licheniformis, bacillus thuringiensis, bacillus laterosporus, bacillus mucilaginosus, streptomyces jingyangensis, mycorrhiza fungi, azotobacter vinelandii, photosynthetic bacteria, bacillus coagulans, aspergillus oryzae, and paecilomyces lilacinus. According to the flora for recycling treatment of organic wastes, disclosed by the invention, by virtue of mutual promotion, mutual coordination and combined action of a variety of beneficial microorganisms, the organic wastes are recycled, environmental pollution is reduced, and wastes are changed into valuables; and the flora for recycling treatment of organic wastes can be used for producing industrial fermentation products and improving the health and growth conditions of animals, and can also improve the current situation of serious soil hardening at present and improve soil quality, thereby promoting the rapid development of agricultural economics.

The invention discloses pig-chicken excrement fermenting compound bacteria, a preparation method and a method of utilizing the compound bacteria to ferment and prepare an organic fertilizer. The fermenting compound bacteria comprise the following raw material bacteria: bacillus subtilis, bacillus licheniformis, bacillus coagulans, bacillus megatherium and trichoderma viride. The method for preparing the organic fertilizer comprises the following steps: mixing pig-chicken excrement with crushed straws, adding the fermenting compound bacteria to the mixture according to a dosage of 1-2 Kg per ton, mixing uniformly, the moisture being 50-65 wt%, stacking the mixed raw materials, keeping the temperature at 55-65 DEG C to conduct fermentation, two days after the fermentation, turning the stacked materials for the first time, and then turning the materials once every one or two days, keeping the moisture at 50-65 wt% during the fermentation phase, and after fermentation, obtaining the organic fertilizer with a moisture content of 20-30 wt%. The pig-chicken excrement fermenting compound bacteria disclosed by the invention can be used for fermenting the pig and chicken excrement; compared to the prior art, the method for preparing the organic fertilizer is low in productive investment and production cost, convenient to use, free of odor, and good in the fermentation and decomposing effect.

The invention relates to a biological agent for processing industrial sewage, a preparation method and application. The biological agent is prepared by the following raw materials in percentage by weight; 25% of rhodococcus, 20% of bacillus subtilis, 15% of pseudomonas aeruginosa, 10% of saccharomyces cerevisiae, 10% of nitrosobacteria, 10% of bacillus megatherium, 5% of aspergillus niger and 5% of paracoccus denitrificans. According to the biological agent provided by the invention, the strains are reasonably matched, symbiotic and coordinative and out of antagonism; after being added to a sewage treatment system, the trains are good in effect of degrading macromolecular toxic and harmful substances which are hardly degraded, such as phenols, aromatichydrocarbons, ammonia nitrogen and phosphorus; and the biological agent has unique effect of processing high-concentration organic sewage and high-ammonia nitrogen sewage which are hardly processed by conventional activated sludge process.

The invention discloses a compound microbial fertilizer for antagonism of soil-borne fungal diseases, and a preparation method and an application thereof; the fertilizer takes humic acid as a compound carrier, includes bacillus amyloliquefaciens HFJ-7 with the bacteria content of 1.8*10<8>-2.1*10<8> CFU/g, and also includes bacillus megatherium B3. The bacillus amyloliquefaciens HFJ-7 in the compound microbial fertilizer has the preservation number of CGMCC No.10011. The bacillus amyloliquefaciens HFJ-7 strain can be antagonistic to rhizoctonia solani, fusarium oxysporum, root rot pathogen, cucumber fusarium wilt pathogen, phytophthora capsici leon pathogen and other soil-borne disease pathogenic fungi. After the bacillus amyloliquefaciens HFJ-7 is compounded with the bacillus megatherium B3, the resistance to rhizoctonia solani and pepper root rot pathogen can be significantly improved, a disease state index of pepper seedling root rot disease can be significantly reduced, and the occurrence and spreading of pepper root rot can be effectively prevented and controlled.

The invention discloses a leavening agent for poultry excrement, which comprises following microorganism fermentation liquor by volume ratio: 30-40% of bifidobacterium, 10-25% of lactic acid bacteria, 10-30% of bacillus megatherium, 10-25% of saccharomycetes, 10-15% of actinomycetes and 10-15% of acetic bacteria. The leavening agent can eliminate environmental fetor and restrain multiplication of malignant bacteria. Organic garbage in treated in composting mode, thereby recycling resources, reducing environment pollution, reducing sewer dirt and reducing odor. The poultry excrement is fermented into fertilizers and animal feed. The leavening agent for the poultry excrement is widely applied to fermentation of excrement and garbage.

The invention discloses a saline-alkali soil biological improved fertilizer based on high-sulfur gangue. The saline-alkali soil biological improved fertilizer is prepared from the following components in parts by weight: 50-60 parts of high-sulfur gangue microbial fermentation product, 20-30 parts of compost cow manure or chicken manure, 1-2 parts of citric acid, 1-2 parts of oxalic acid, 2-4 parts of urea, 2-4 parts of ammonium sulfate, 2-4 parts of monoammonium phosphate, 1-3 parts of potassium sulphate, 2-5 parts of ferrous sulfate, 1-2 parts of zinc sulfate, 1-2 parts of manganese sulfate and 1-10 parts of bentonite, wherein the high-sulfur gangue microbial fermentation product is a product obtained by performing activating treatment on the high-sulfur gangue by a compound microorganism bacterium solution composed of thiobacillus thiooxidans, bacillus subtilis, bacillus megatherium, bacillus mucilaginosus and enterococcus faecalis. The resource utilization of the high-sulfur gangue is realized, the improvement of the inland saline land can be promoted, the crop yield can be increased and the entironment can be improved.

The invention relates to a compound microbial preparation for promoting paddy growth and a preparation method thereof. The compound microbial preparation for promoting paddy growth is characterized by consisting of 20-40 parts by weight of a microbial strain culture and 60-80 parts by weight of a microbial carrier; wherein, the microbial strain culture consists of the following raw materials in parts by weight: 2-8 parts of bacilli subtilis, 2-8 parts of bacilli megaterium, 5-10 parts of saccharomyces cerevisiae and 1-4 parts of rhizobia; the microbial carrier is zeolite or bentonite or mixture thereof. The compound microbial preparation has the advantages of promoting growth of beneficial microbes, enhancing absorption of nitrogen, phosphorous, potassium and other organic substances for paddy root system and promoting normal growth of the root system, thus promoting the paddy growth and reducing chemical fertilizer application.

The invention discloses an immobilized microbial preparation which is prepared through the following steps: S1, fermentation culturing strain: respectively inoculating bacillus subtilis, bacillus megatherium, nitrobacter and nitrosomonas in an enrichment medium to be cultured; S2, mixing thallus to obtain mixed thallus liquor; S3, immobilizing PVA-sodium alginate, namely uniformly mixing PVA gel with the mixed thallus liquor, dripping the mixture into a boric acid solution containing CaCl2 while continuously stirring at a low speed, immobilizing and crosslinking the mixture, taking out the mixture, washing the mixture through deionized water, and freeze drying the mixture to obtain the immobilized microbial preparation. The invention further discloses a method of treating meat product processing waste water by utilizing the immobilized microbial preparation. The immobilized microbial preparation disclosed by the invention has the beneficial effects that the microorganism categories are various, various pollutants in the waste water can be degraded, the degrading speed is fast and the capability is strong; the immobilized microbial preparation is suitable for changes of water quality and pH; the sludge yield is small, the products are liable to separate, the stability is good, the technique operation is simple, and the production cost is low.

The invention discloses a facultative anaerobic fermentation fungicide. The facultative anaerobic fermentation fungicide is prepared from facultative anaerobic fermentation flora. The facultative anaerobic fermentation flora is prepared from lactic acid bacteria, lactobacillus acidophilus, bacillus subtilis, bacillus licheniformis, bacillus coagulans, aspergillus oryzae, bacillus megatherium, bacillus laterosporus, bacillus thuringiensis, streptococcus faecium, photosynthetic bacteria, saccharomycetes, paecilomyces lilacinus, nitrogen-fixing bacteria and actinomycetes. The facultative anaerobic fermentation fungicide can be suitable for facultative anaerobic fermentation of organic fertilizer, organic matter can be efficiently converted, and growth of pernicious bacteria can be inhibited. The invention also discloses a preparing method of the organic fertilizer, in the method, facultative anaerobic fermentation is carried out through the facultative anaerobic fermentation fungicide, the fermentation cycle is short, the running cost is low, the organic fertilizer prepared with the method is rich in nutriment, environmentally friendly and harmless, and development of ecological agriculture can be promoted.

The invention discloses a biological special organic fertilizer applied to crops, in particular to a stubble regulating manure that eliminates the block of continuous cropping. The mixture ratio of rotten microbial inoculum is 30 percent of thermophil Bacillus subtilis, 20 percent of Bacillus megatherium, 10 percent of microzyme, 10 percent of Aspergillus flavus, 10 percent of seminude chactomium and 20 percent of Absidia corymbifera; the components of organic fertilizer are of 40 percent of chicken manure, 30 percent of dairy manure and 30 percent of straw powder; the process flow is that: the first fermentation is to graft rotten microbial inoculum in high quality organic fertilizer chicken manure and dairy manure to reach the standard of innocent treatment; the second fermentation is to take the organic fertilizer in the first fermentation as raw material and graft functional bacterial strain to lead the beneficial bacterial count in the organic fertilizer to reach the standard of biological fertilizer. As the invention chooses beneficial strain and adopts unique two-fermentation process, the produced stubble regulating manure can greatly adjust soil ecological balance, inhibit soil-borne disease, decrease the side effect of fertilizer and agricultural chemical, provide full nutrition for the growth of crops and improve the quality of agricultural products.

The invention discloses a microbial preparation for improving heavy metals in soil. The microbial preparation comprises photosynthetic bacteria, actinomycetes, lactic acid bacteria, saccharomycetes, bacillus subtilis, bacillus thuringiensis, bacillus megatherium, nitrogen-fixing bacteria, citric acid germs, bacillus licheniformis and a matrix for providing nutrition for the flora. The microbial preparation for improving the heavy metals in soil can degrade the heavy metals in the soil safely and efficiently in an environment-friendly manner, and can meet the demand for social and economic development.

The invention relates to a biological organic fertilizer special for cucumber. Through the biological organic fertilizer, cucumber growth can be promoted, the cucumber yield can be increased and the incidence of soil-borne diseases of cucumber can be reduced. The biological organic fertilizer comprises a basal fertilizer and a top dressing, wherein the basal fertilizer consists of the following components in parts by mass: 300-600 parts of rotten chicken manure, 50-200 parts of grass carbon, 50-200 parts of weathered coal, 50-100 parts of molasses powder, 0-50 parts of urea, 10-100 parts of diammonium hydrogen phosphate, 100-200 parts of potassium sulfate and 20-100 parts of kaolin; the top dressing consists of the following components in parts by mass: 300-500 parts of rotten chicken manure, 50-200 parts of grass carbon, 50-200 parts of weathered coal, 50-100 parts of molasses powder, 100-150 parts of urea, 150-250 parts of potassium sulfate and 20-100 parts of kaolin; and bacillus megatherium, bacillus mucilaginosus and paenibacillus polymyxa are added into both the basal fertilizer and the top dressing.

The invention discloses a saline-alkali soil modified microbial inoculum and a preparation method thereof. The microbial inoculum comprises, by weight, 10-30 parts of bacillus megatherium HB bacteriapowder, 35-65 parts of phosphorus tailings powder, 12-18 parts of phosphogypsum, 8-25 parts of sugar refinery wastes, 6-26 parts of seaweed residue and 1-8 parts of mannitol. Components of the saline-alkali soil modified microbial inoculum have synergistic interactions with each other to enhance effects, if any one of the components lacks, expected effects cannot be reached, and only when the saline-alkali soil modified microbial inoculum with whole formulas is adopted and 30-80 kg of the microbial inoculum is used per mu, can the effects of increasing fertilizer utilization rate and crop yields, and improving quality and saline-alkali lands be achieved.