77 results about "Bacillus megatherium" patented technology

The invention relates to the field of self-repairing concrete, in particular to high-strength self-repairing concrete and a preparation method thereof. The high-strength self-repairing concrete comprises the following substances in parts by weight: 45-60 parts of cement; 100 to 120 parts of recycled coarse aggregate; 65 to 80 parts of fine aggregate; 10 to 20 parts of water; 0.1 to 0.3 part of a water reducing agent; the recycled coarse aggregate is the recycled coarse aggregate immobilized with self-repairing microorganisms, and the self-repairing microorganisms comprise at least one of bacillus subtilis, bacillus megatherium and bacillus pasteurii. The concrete crack self-repairing capability is endowed through the immobilized self-repairing microorganisms. The microorganisms form resting spores in the concrete, when cracks are generated and water permeates into the cracks, the microorganisms are activated and metabolize a calcium source and urea in the surrounding environment, calcium carbonate crystals are generated through the mechanism that the microorganisms induce carbonate precipitation, the cracks are filled, and the structural integrity is recovered. The process does not need external intervention, and the mechanical structure and mechanical properties of the concrete structure can be remarkably improved.

The invention discloses a rapid restoration method for degraded grassland based on a microbial agent. The microbial agent is formed by compounding 15-30 parts of a nitrogen-fixing microbial agent, 10-20 parts of a phosphate-solubilizing microbial agent, 8-15 parts of a plant growth-promoting microbial agent, 25-40 parts of a humic acid carrier, 3-8 parts of an embedding agent sodium alginate and 5-12 parts of a nutritional agent molasses. Wherein the nitrogen-fixing bacteria are azotobacter chroococcum and alfalfa rhizobium (the viable count is greater than or equal to 5 * 10 < 9 > CFU / g), the phosphate solubilizing bacteria are bacillus megatherium (the viable count is greater than or equal to 1 * 10 < 10 > CFU / g), and the growth-promoting bacteria are bacillus subtilis and pseudomonas fluorescens (the viable count is greater than or equal to 2 * 10 < 10 > CFU / g). The microbial inoculum is subjected to activation, carrier adsorption, sodium alginate embedding granulation and CaCl2 cross-linking processes to be prepared into slow-release particles of 1-3 mm. 200-250 kg of organic fertilizer is applied to each hectare in a mild area, 300-400 kg of organic fertilizer is applied to each hectare in a moderate and severe area, and leymus chinensis and wheatgrass are sown in a mixed mode. After 90 days of application, the vegetation coverage is improved by 40% or above, the soil organic matter is increased by 25-40%, the microbial diversity index is larger than or equal to 3.5, and the comprehensive remediation cost is reduced by 53%.

The invention provides bacillus megaterium and application thereof in preparation of a nano-selenium organic fertilizer and improvement of the selenium content of tea leaves, and belongs to the technical field of microorganisms, the bacillus megaterium NT2 is preserved in the China Center for Type Culture Collection with the preservation number of CCTCC NO: M 20251806, and the bacillus megaterium NT2 is preserved in the China Center for Type Culture Collection with the preservation number of CCTCC NO: M 20251806. The bacillus megatherium NT2 has relatively strong resistance to reduction on selenium element, can survive in high-concentration inorganic selenium, can reduce the inorganic selenium into nano-selenium, and has relatively weak capability of enriching arsenic, cadmium and lead; the selenium source of the nano-selenium organic fertilizer can be prepared by mixed fermentation with high-selenium rock powder which is calcined and activated at high temperature, so that the content of heavy metals does not exceed the national standard while the selenium content of the tea leaves is increased. The bacillus megaterium provided by the invention not only can reduce the toxicity of inorganic selenium in high-selenium rock and convert the inorganic selenium into nano-selenium, but also can stably, safely and standardly produce selenium-rich tea leaves, the heavy metal content of the tea leaves does not exceed the standard, and the bacillus megaterium has a wide market prospect.

The invention discloses a bacillus megaterium Q7 for promoting the growth of leucaena leucocephala and improving the abundance of a probiotic microbial population. The bacillus megaterium Q7 is a bacillus megaterium for promoting the growth of leucaena leucocephala. Belongs to the technical field of microorganisms. The bacillus megatherium Q7 provided by the invention improves the conversion from total-effect nutrients to effective nutrients, and enhances the consumption of the leucaena leucocephala on soil nutrients, thereby achieving the effect of promoting the growth of the leucaena leucocephala. After the bacillus megatherium Q7 provided by the invention is applied, the enzyme activity of soil is remarkably improved, the conversion of available nutrients in the soil is remarkably enhanced, and the nutrient consumption capacity of leucaena leucocephala is enhanced. The bacillus megaterium Q7 is applied to promote the growth and development of the leucaena leucocephala, so that the contents of soil hydrolytic nitrogen and available phosphorus are remarkably increased, and the conversion to soil nutrients is enhanced. In the genus level, the average relative abundance of bacillus treated by the bacillus atrophaeus Q7 is increased to 9.97% from 1.33%, the bacillus pseudobecomes the leucaena leucocephala soil community dominant bacteria genus, and the relative abundance of Fictibacillus is increased by about 6 times, which indicates that the inoculation of the strain Q7 enables the leucaena leucocephala soil community structure to be changed, and the leucaena leucocephala soil community dominant bacteria genus is changed. And the increase of the relative abundance of dominant bacteria is promoted. Wherein the bacillus and the Fictibacillus belong to obvious increase of relative abundance and are possibly an important factor for promoting plant growth.

The invention relates to a microbial agent for preventing and treating tomato fusarium wilt caused by fusarium oxysporum and promoting plant growth. The microbial agent comprises a microbial flora constructed by five strains including bacillus amyloliquefaciens, bacillus velezensis, paenibacillus polymyxa, bacillus megatherium and bacillus subtilis. The synthetic flora provided by the invention can effectively antagonize Fusarium oxysporum, the inhibition rate of the Fusarium oxysporum can reach 64-99%, the inhibition capability of the synthetic flora is obviously superior to that of single strains A, B, C, D and E, and the biomass of tomatoes can be obviously improved.

The invention relates to the technical field of fertilizers, in particular to a microbial fertilizer for a seedling raising period of raspberries and a preparation method of the microbial fertilizer. The preparation method of the microbial fertilizer for the raspberry seedling raising period comprises the following steps: (1) mixing and fermenting antibacterial bacteria, plant growth-promoting rhizobacteria, phosphate solubilizing bacteria and nitrogen-fixing bacteria according to a mass ratio to obtain a microbial compound bacteria solution; (2) mixing and stirring the microbial compound bacteria liquid, a nutrient additive and a carrier to obtain the microbial fertilizer for the seedling raising period of the raspberries, the antibacterial bacteria are salt-tolerant bacillus; the plant growth-promoting rhizobacteria is enterobacter lewis; the phosphate solubilizing bacteria are bacillus megatherium; the nitrogen-fixing bacteria are azospirillum brasilense. The microbial fertilizer disclosed by the invention can improve the survival rate of raspberry seedlings and the yield of a single raspberry plant, also can improve the fertility of soil, improve the structure of the soil, increase the content of organic matters and nitrogen in the soil, effectively reduce the production cost of secondary planting, and achieve the beneficial effects of reducing cost and improving efficiency.

The invention belongs to the technical field of microbial control, and particularly relates to a compound microbial agent as well as a preparation method and application thereof. The preparation method of the compound microbial agent comprises the following steps: (1) respectively activating bacillus megatherium, bacillus marinus N6-2 and lactobacillus plantarum A37 to obtain various strain activating solutions; mixing the activation solutions of the strains to prepare a mixed bacterial solution; (2) inoculating the mixed bacteria solution into a seed culture medium to prepare a seed solution; and (3) inoculating the seed solution into a fermentation culture medium for fermentation, and then drying and crushing to obtain the compound microbial agent. According to the prepared compound microbial agent, the total number of effective viable bacteria is increased by optimizing the composition of the culture medium. After the compound microbial agent is used, the contents of organic matters, quick-acting nitrogen, quick-acting phosphorus and quick-acting potassium in soil can be remarkably increased, and the soil fertility is improved, so that the growth of tomatoes is promoted, the yield and quality of the tomatoes are improved, and the compound microbial agent has a wide application prospect.

The invention discloses a combined preparation for improving rice quality and application thereof, and belongs to the technical field of crop planting. The combined preparation is prepared from the following raw materials in parts by weight: radix angelicae sinensis, radix paeoniae alba, rhizoma atractylodis macrocephalae, fructus forsythiae, oyster mushroom bran, needle mushroom bran, a microbial agent, ammonium nitrate, potassium nitrate, ammonium molybdate, DTA-6, zinc sulfate and monopotassium phosphate. The microbial agent is prepared from bacillus megatherium, pseudomonas putida, serratia marcescens and saccharomycetes. The combined preparation for improving the rice quality is prepared by taking natural Chinese herbal medicines, fungus chaff, microbial strains and a plant growth regulator as raw materials. The combined preparation is rich in polysaccharide, amino acid, chitosan oligosaccharide and other components and active microbial agents, and can promote the growth and development of rice and improve the quality of rice. The combined preparation provided by the invention provides a new technical thought for rice quality improvement.

The invention relates to the technical field of toluene degradation treatment, and discloses an immobilized microbial inoculum capable of degrading toluene as well as a preparation method and application of the immobilized microbial inoculum. The preparation method comprises the following steps: adding a bacillus megatherium activated bacterium solution into a liquid culture medium, and adding nano carbon black powder to obtain carbon black immobilized bacteria; heating and stirring the carboxymethyl xylan zinc complex and the nano magnesium lithium silicate until the mixture is molten; after cooling, adding the nano carbon black immobilized bacteria to obtain a mixed solution; slowly dropwise adding the mixed solution into a calcium chloride solution to form embedded pellets; and washing to obtain the immobilized microbial inoculum. According to the immobilized microbial inoculum capable of degrading toluene and the preparation method and application thereof, a strain is embedded in CXY-Zn / nLMS gel and gradually released through gel beads, the biological activity of the strain is better maintained, meanwhile, carbon black of a porous structure provides a place for attachment and growth of the strain, the service life of the strain is prolonged, the degradation capacity of microorganisms is improved, and the immobilized microbial inoculum can be used for degrading toluene. The prepared immobilized microbial agent can be used for sewage treatment and soil improvement.

The invention discloses application of bacillus megaterium and a biological fertilizer thereof in rice planting, and relates to the technical field of microorganisms. The bacillus megaterium is preserved in the China General Microbiological Culture Collection Center on July 10, 2025, and the preservation number of the bacillus megaterium is CGMCC (China General Microbiological Culture Collection Center) NO.35179. The bacillus megaterium has the advantages that the bacillus megaterium is preserved in the China General Microbiological Culture Collection Center on July 10, 2025; the strain is a plant growth-promoting bacterium and has a good rice growth-promoting effect, and when the prepared biological fertilizer is applied to the rice planting process, compared with a control group, the tiller number of rice is increased by 64.44%, the plant height is increased by 2.77%, and the leaf chlorophyll content is increased by 8.38%. The bacillus megatherium provided by the invention and the biological fertilizer prepared by strain fermentation have the advantages of greenness, safety, high efficiency and the like, and have a better application prospect in the aspect of agricultural production.

The invention discloses an acid soil conditioner containing microalgae, and belongs to the technical field of agriculture and environmental restoration. The acid soil conditioner containing the microalgae is prepared from the following components in parts by weight: 100 to 130 parts of biomass charcoal, 30 to 40 parts of vermiculite, 1 to 2 parts of brachyristichum brachyristichum, 0.1 to 0.5 part of a bacillus mucilaginosus microbial agent, 0.1 to 0.5 part of a bacillus subtilis microbial agent and 0.1 to 0.5 part of a bacillus megatherium microbial agent. According to the method disclosed by the invention, the brachyristichum brachyristichum which can be planted under an acidic condition and can generate alkaline metabolites to increase the pH value of acidic soil is selected as a main functional component, and is matched with other components with functions of loosening soil, helping the brachyristichum brachyristichum to proliferate, providing organic matters, helping the soil to release nutrients and the like; and compounding to obtain the soil conditioner capable of effectively improving acid soil.

The invention provides bacillus megatherium for preventing and treating soil-borne diseases of crops, a microbial inoculum and application of the bacillus megatherium and the microbial inoculum. The bacillus megaterium HN-2 disclosed by the invention is classified and named as bacillus megaterium, the bacillus megaterium HN-2 is preserved in the general microbiological center of the China Committee for Culture Collection of Microorganisms, and the preservation number of the bacillus megaterium HN-2 is CGMCC No.34657. A test result shows that the antibacterial effect of the bacillus megaterium HN-2 on four kinds of fusarium and tobacco black shank bacteria reaches 60.98%-71.12%; the indoor nematode killing effect on wheat cyst nematode and tomato root-knot nematode reaches 100%. The optimized binder, wetting agent, stabilizing agent and disintegrating agent are respectively starch, lauryl sodium sulfate, CaCO3 and AlCl3; after seed dressing and broadcasting, the field cyst decline rates of wheat cyst nematode and soybean cyst nematode are 60.22% and 52.58% respectively, the field prevention effects of wheat root rot and soybean root rot are 69.50% and 54.44% respectively, and the biocontrol inoculant has wide application potential.

The invention relates to the field of acid soil conditioners, in particular to a charcoal-based acid soil conditioner as well as a preparation method and application thereof. The biochar-based acid soil conditioner comprises a biochar carrier and a complex microbial inoculant loaded on the biochar carrier, and the complex microbial inoculant comprises 25-35 parts of nitrogen-fixing functional bacteria; 20 to 40 parts of phosphorus and potassium solubilizing bacteria; 15 to 25 parts of nitrogen activating bacteria; 15 to 25 parts of synergistic growth promoting bacteria; the nitrogen-fixing functional bacteria comprise Azospirillum brasilense and / or Klebsiella, the phosphate-solubilizing and potassium-solubilizing bacteria comprise bacillus megatherium and / or bacillus mucilaginosus, the nitrogen-activating bacteria comprise urease-producing bacillus and / or nitrospirillum, and the synergistic growth-promoting bacteria comprise arbuscular mycorrhizal fungi and / or pseudomonas. The complex microbial inoculant is constructed by screening four functional strains of nitrogen fixation, phosphorus and potassium solubilization, nitrogen activation and synergistic growth promotion, a complete functional closed loop of nitrogen fixation, phosphorus solubilization, nutrient activation and crop growth promotion is formed, and synergistic interaction is achieved through functional complementation of the strains.

This invention belongs to the field of bio-mineral fertilizer technology, specifically relating to a microbial liquid compound agent and bio-mineral fertilizer that combine coal gangue activation and heavy metal remediation. The microbial liquid compound agent comprises the following functional strains prepared through three-stage sequential inoculation and fermentation using electrochemical low-Hertz water: *Pseudomonas putida* (a remedial bacterium), *Azotobacter chrysogenum* (a nitrogen-fixing bacterium), *Azospirillum brasiliensis* (a growth-promoting bacterium), *Pseudomonas fluorescens* (a phosphate-solubilizing bacterium), *Bacillus subtilis* (a biocontrol bacterium), *Bacillus mucilaginosus* (a potassium-solubilizing bacterium), *Bacillus megaterium* (a silica-solubilizing bacterium), and *Trichoderma echinococcus* (a synergistic bacterium). The bio-mineral fertilizer is prepared using the aforementioned microbial liquid compound agent, with coal gangue powder as the main raw material. The scientific combination of different strains provided by this invention achieves efficient improvement of heavy metal-contaminated land and increased crop yields. Simultaneously, it transforms coal gangue into a valuable resource, solving the pollution problem caused by the large-scale accumulation of coal gangue, and possesses significant economic and social value.

The invention discloses a self-propagating microbial soil conditioning agent and a preparation process thereof, and relates to the technical field of soil treatment. The invention relates to a self-propagating microbial soil conditioning agent. Comprising the following components in parts by mass: 13 to 21 parts of Orson's bacteria, 11 to 22 parts of bacillus licheniformis, 10 to 15 parts of bacillus mucilaginosus, 20 to 23 parts of bacillus megatherium, 15 to 25 parts of bacillus thuringiensis, 15 to 20 parts of bacillus laterosporus, 12 to 25 parts of bacillus mucilaginosus and 240 to 365 parts of plant organic matters. The colonic microorganism soil conditioning fungicide prepared from the components can remarkably improve the soil structure, improve the soil fertility and promote crop growth. Meanwhile, due to the fact that microorganisms have the self-breeding capacity, beneficial microbial communities can be continuously provided for the soil, and the ecological balance of the soil is maintained. In addition, the microbial agent also has the advantages of preventing and treating soil diseases, reducing the use amount of chemical fertilizers and the like, and has important significance for promoting agricultural sustainable development.

This invention relates to a novel Bacillus megatherium HyangYak-01 strain and its applications. According to one embodiment of the novel Bacillus megatherium HyangYak-01 strain, it exhibits effects such as increasing the diversity of microbiome species in soils used for crop cultivation or improving their physicochemical properties, and promoting crop growth or the biosynthesis of physiologically active substances. Therefore, it can be applied to a variety of uses, such as improving soil conditions, increasing crop cultivation efficiency, or as a fertilizer to enhance crop efficacy.

The invention discloses a microbial fertilizer for preventing and treating citrus liberobacter asiaticum as well as a preparation method and application thereof, belongs to the technical field of prevention and treatment of agricultural diseases and pests, and particularly relates to the microbial fertilizer for preventing and treating the citrus liberobacter asiaticum. Comprising 5-10 parts of bacillus subtilis, 5-10 parts of bacillus amyloliquefaciens, 3-8 parts of bacillus megatherium, 3-8 parts of bacillus licheniformis, 3-8 parts of photosynthetic bacteria, 3-8 parts of nitrogen-fixing bacteria, 3-8 parts of phosphate solubilizing bacteria, 3-8 parts of potassium bacteria, 30-50 parts of decomposed organic fertilizer, 10-20 parts of humic acid, 5-10 parts of sodium alginate, 5-10 parts of compound amino acid and 1-5 parts of compound trace elements. The microbial fertilizer disclosed by the invention can effectively inhibit the growth of pathogenic bacteria of the citrus huanglongbing, improve the disease resistance of citrus trees, improve the soil environment and promote the growth and development of the citrus trees, and the preparation method is mature and perfect in process and suitable for large-scale production and application.

The invention discloses a microbial agent and application thereof in pepper planting. According to the microbial agent, bacillus subtilis and bacillus megatherium are subjected to independent liquid fermentation, auxiliary materials and a carrier are added, and the microbial agent is prepared for pepper planting. Wherein in the liquid fermentation process, waste liquid obtained after trehalose production is adopted as one of production raw materials for high-density fermentation, the bacterial colony number of the final liquid fungicide is larger than or equal to 1.4 * 10 < 9 > cfu / mL, the content of polyglutamic acid is larger than or equal to 32.5 g / L, and the titer of the antibacterial peptide is larger than or equal to 600 U / mL. The microbial agent can be applied to pepper planting, the pepper growth cycle is short, the leaf surfaces are emerald green and dense, the acre yield of the pepper can be remarkably increased, the vitamin C content in the pepper is remarkably increased to 967.5 mg / Kg, and the soluble sugar content in the pepper is remarkably increased to 3.43%.

The application discloses a high-phosphorus hematite biological desphosphorization method based on micro-electric field stepwise domestication and nutrient slow-release regulation, and belongs to the technical field of mineral processing and bio-metallurgy. The biological desphosphorization method comprises the following steps: culturing Bacillus subtilis and Bacillus megaterium to the logarithmic growth phase to obtain active bacteria suspension of a combined bacterial flora; placing the active bacteria suspension in different high-phosphorus hematite slurries from low concentration to high concentration, and obtaining a dominant functional bacterial flora under the regulation of micro-electric field and nutrient slow release; culturing the dominant functional bacterial flora to the logarithmic phase, then adding pretreated high-phosphorus hematite powder for leaching, monitoring the pH value, and separating to obtain leaching solution I and leaching residue I; mixing the leaching residue I with a fungal leaching medium, inoculating a fungus Talaromyces for synergistic leaching, and separating to obtain leaching solution II and desphosphorized iron concentrate. The application successfully constructs an efficient, stable and environment-friendly functional bacterial flora + fungus synergistic system, and realizes efficient removal of phosphorus elements in high-phosphorus iron ore in a short period.

Disclosed in this disclosure are Acid Sulfate Soil (ASS) improvement materials, and preparation methods and application methods thereof. ASS improvement materials include mixed particle improvement materials and microbial improvement materials. The mixed particle improvement materials include natural mineral materials, organic carbon materials, lignosulfonates, silicates, high molecular polymers, shell powder, fish bone powder, and hydrated lime powder; and the microbial improvement materials include Bacillus megaterium, Bacillus subtilis, Bacillus licheniformis, Bacillus amyloliquefaciens, Trichoderma harzianum, yeast, and photosynthetic bacteria.During use, the mixed particle improvement material is evenly distributed in the soil, and is well mixed with the soil through rotary tillage; the microbial improvement material is dissolved in water and added to the irrigated land, the water surface depth is 5-10 cm, the land is continuously soaked for 5-7 days, and then agricultural cultivation activities are carried out after the water level naturally decreases. The ASS improvement material in the present disclosure has the effects of deacidification, maintenance and improvement of fertilizer, and increase in production, and has the characteristics of efficiency, green, environmental friendliness, etc.

The invention discloses a hydrogel and probiotic compounded microbial inoculum composition and a vegetable planting matrix, a three-dimensional gel carrier is used for embedding a microbial inoculum, the carrier integrates mechanical properties and thallus protection and can maintain flora activity for a long time, bacillus thuringiensis has a low-oxygen survival characteristic after being subjected to Fnr enhanced expression, and the microbial inoculum composition has the advantages that the microbial inoculum composition has a low-oxygen survival characteristic after being subjected to Fnr enhanced expression; the microbial agent composition can survive for a long time in a low-oxygen environment of a carrier embedding environment, and is cooperated with bacillus cereus, azotobacter chroococcum and bacillus megatherium to promote the growth of plants such as vegetables and the like and directionally kill pathogenic microorganisms such as root-knot nematode. Corresponding pests can be killed in a green and targeted manner, the growth of plants is promoted, and the yield is increased.

The invention discloses a microbial agent for in-situ treatment of enteromorpha in an open sea area as well as a preparation method and application of the microbial agent. The microbial agent is naturally formed by fermentation of a composite flora under a proper condition, and the pH value of the microbial agent is less than 4.0; the composite flora comprises bacillus subtilis, nitrifying bacteria, actinomycetes, filamentous bacteria, photosynthetic bacteria, lactobacillus plantarum, saccharomyces cerevisiae, candida, lactobacillus paracasei and bacillus megatherium, and can be applied to differential in-situ treatment of enteromorpha in different scenes (beaches, sea floats and cabins) in open sea areas. The microbial agent is an ecological treatment microbial agent, an acidity chemical regulator is not added, the enteromorpha prolifera in the open sea area can be treated in situ in a strong mode within a short time, and no negative influence is caused to seawater and the marine environment.

This application provides a screening method and animal model related to intraocular diseases, such as age-related macular degeneration (AMD), for identifying candidate therapeutic agents for treating or preventing eye diseases, such as AMD. [Solution] The present application also provides compounds / compositions that can kill or inhibit the growth of microorganisms such as Bacillus megatherium. The present application further provides methods for treating infections caused by microorganisms such as Bacillus megatherium, and for treating or preventing diseases or disorders associated with such infections, such as AMD, using the compounds / compositions.

The invention discloses a microorganism for treating moss and foul smell in park landscape lake water and a culture process thereof, the microorganism comprises biphase photosynthetic bacteria, decomposing bacteria and a culture medium, the decomposing bacteria comprise bifidobacterium, mesophilic actinomycetes, nitrosobacteria and bacillus megatherium, the weight percentage of the decomposing bacteria is 1-5%, and the weight percentage of the decomposing bacteria is 1-5%. The microbial inoculant comprises the following components in percentage by weight: 20-30% of biphasic photosynthetic bacteria, 15-25% of bifidobacterium, 10-20% of mesophilic actinomycetes, 10-20% of nitrosobacteria and 15-25% of bacillus megatherium. The biphase photosynthetic bacteria and the composite decomposition bacteria are scientifically proportioned to form a microbial system with complementary functions, the biphase photosynthetic bacteria can directionally inhibit photosynthesis of moss, destroy cell structures of moss and realize targeted killing, and the decomposition bacteria synergistically decompose organic matters such as underwater residual feed, excrement, animal and plant bodies and the like, so that the biological activity of moss is improved. Meanwhile, nitrosobacteria can convert algal toxins into harmless substances, the synergistic effect overcomes the defects that an existing single microbial preparation is single in function and incomplete in treatment, and integrated treatment of killing moss, eliminating stink and improving water quality is achieved.

The present application relates to the technical field of bio-organic fertilizer preparation, and particularly relates to a bio-organic fertilizer prepared by microbial fermentation and a preparation method thereof, which is prepared by fermentation of raw materials and compound microbial strains, wherein the raw materials include livestock and poultry manure, crop straw, industrial organic waste residue and a humification agent, and the compound microbial strains are a ternary combination of bacillus subtilis, bacillus mycoides and saccharomyces cerevisiae, or a quaternary combination of bacillus subtilis, bacillus mycoides, saccharomyces cerevisiae and bacillus mucilaginosus. The present application adopts a multi-element compound strain combination and optimizes the ratio, and the synergistic effect of each strain is significant, which greatly improves the organic matter degradation efficiency and effective nutrient activation capacity, and compared with single strain, the microbial activity is stronger, and the nutrient supply capacity of the fertilizer is better.

The invention relates to the technical field of soil remediation, in particular to a saline-alkali soil remediation fungicide and a preparation method thereof. Comprising the following raw materials in parts by weight: 4 to 8 parts of bacillus subtilis, 8 to 15 parts of purple sulfur bacteria, 10 to 17 parts of rhizobium, 1 to 3 parts of bacillus megatherium, 1 to 3 parts of candida tropicalis, 1 to 3 parts of aspergillus niger, 0.05 to 0.2 part of proline, 0.5 to 2.5 parts of humic acid, 3 to 5 parts of modified charcoal, 0.1 to 0.5 part of glucose, 0.4 to 1.2 parts of monopotassium phosphate and 0.3 to 0.6 part of calcium chloride. The application of the soil remediation fungicide not only improves the growth environment of the crops, but also obviously improves the salt tolerance of the crops by adjusting the stress resistance mechanism of the crops. After the soil remediation fungicide is applied, the root system development of crops is more robust, and the density and length of the root system are obviously increased, so that the crops can absorb water and nutrients from the soil deeper layer, and the surface salt damage can be effectively resisted.

The invention relates to a microbial agent with a deodorization effect as well as a preparation method and application of the microbial agent. The bacterial strains in the microbial agent comprise a bacillus megaterium YBG02 bacterial strain with the preservation number of CCTCC (China Center for Type Culture Collection) NO: M2021052 and a bacillus subtilis YBS01 bacterial strain with the preservation number of CCTCC NO: M2021053. The bacillus megaterium and the bacillus subtilis both belong to strains safe to human, animals and the environment and have an obvious odor removal effect, and the prepared microbial inoculum can be widely applied to cat litter production and has the advantages of being good in temperature resistance and low in loss rate.

The present invention discloses a soil-improving microbial fertilizer containing Bacillus megaterium and a preparation method thereof, belonging to the technical field of fertilizers. The soil-improving microbial fertilizer containing Bacillus megaterium comprises the following raw materials in parts by weight: 35-50 parts of an immobilized bacterial agent, 3-5 parts of a water-retaining agent, 10-15 parts of humic acid, and 2-5 parts of trehalose; the immobilized bacterial agent is amino-MOF-modified biochar loaded with Bacillus megaterium; the water-retaining agent is a water-absorbing resin carrying a phosphate group. The microbial fertilizer of the present invention comprises the immobilized bacterial agent, the water-retaining agent, the humic acid, and the trehalose, has strong targeting, and performs outstandingly in soil improvement. On the one hand, it activates available phosphorus in reclaimed soil to provide required nutrients for plants; on the other hand, it activates and regenerates soil organic matter, improves soil fertility, and meets plant nutrient requirements.

The invention relates to the technical field of microbial fertilizers, in particular to a compound microbial fertilizer for soil improvement and a green preparation method thereof.The compound microbial fertilizer is prepared from screened and purified compound bacteria, EM bacteria, bacillus megatherium, bacillus mucilaginosus, photosynthetic bacteria, mesoporous silica, humic acid and fermented cottonseed meal; screened and purified compound bacteria are introduced, are obtained by screening and purifying cow dung, and contain a plurality of microbial strains with specific functions. The bacterial strains can form dominant bacterial communities in soil, inhibit growth of harmful microorganisms through a competitive effect, regulate a soil microbial community structure and improve a soil micro-ecological environment, and the compound microbial fertilizer can improve a soil physical structure, regulate soil chemical properties and a microbial environment and improve the soil micro-ecological environment through synergistic cooperation of all the components, so that the compound microbial fertilizer has the advantages of low cost and high efficiency. The problems of soil hardening, physicochemical property reduction and the like caused by long-term use of chemical fertilizers are effectively solved.

The invention relates to the technical field of flower planting, in particular to a beneficial bacterium nutrient solution for promoting orchid growth and a preparation method and application thereof. The beneficial bacterium nutrient solution disclosed by the invention is prepared from the following components in parts by mass: 0.02 to 0.05 part of a bacillus megaterium bacterium agent, 0.02 to 0.06 part of a bacillus amyloliquefaciens bacterium agent, 0.3 to 0.8 part of bacillus coagulans, 0.1 to 0.5 part of a bacillus subtilis bacterium agent, 0.04 to 0.08 part of a lactobacillus plantarum bacterium agent, 0.5 to 1.0 part of trichoderma harzianum, 2 to 8 parts of humic acid, 1 to 5 parts of potassium fulvate, 2 to 8 parts of sodium alginate, 4 to 10 parts of monopotassium phosphate and 1800 to 2000 parts of waste liquid. The beneficial bacterium nutrient solution prepared from the components can promote growth of orchid roots, increase the number of orchid flowering branches and prolong the flowering period of orchid.