105 results about "Acinetobacter" patented technology

The invention belongs to the technical field of antibacterial peptides, and particularly relates to an LL-37 derived antibacterial peptide and application thereof. According to the invention, 17-29aa of the LL-37 antibacterial peptide is used as a core fragment and is connected with an amino acid sequence shown as SEQ ID NO: 1 or SEQ ID NO: 2 through an amido bond, the antibacterial activity of the obtained antibacterial peptide is obviously higher than that of the LL-37 antibacterial peptide, and the antibacterial peptide shows an obvious bactericidal effect on clinical drug-resistant bacteria such as acinetobacter baumannii, pseudomonas aeruginosa, escherichia coli, staphylococcus aureus, klebsiella pneumoniae and the like; meanwhile, the cytotoxicity and hemolytic activity are weak, drug resistance is not prone to being generated, bacteria can be rapidly killed in vivo through multiple mechanisms, no obvious cytotoxicity exists, the bacterium load in tissue is remarkably reduced, and the lifetime of a bacterium-infected mouse is prolonged.

The invention discloses a phycomycete composite biological fertilizer for an arid loess area as well as a preparation method and application of the phycomycete composite biological fertilizer, and belongs to the technical field of biological fertilizers. The phycomycete composite biological fertilizer is prepared from nitrogen-fixing anabaena and acinetobacter, counted by a single cell, the total viable count of the anabaena azotica is greater than or equal to 1.0 * 10 < 6 > CFU / mL, and the total viable count of the acinetobacter is greater than or equal to 1.0 * 10 < 7 > CFU / mL. Experiments prove that the phycomycete composite biological fertilizer can significantly improve the germination potential and germination rate of crop seeds, promote seedling growth, improve the nutritional quality of crops and reduce the death rate of seedlings, is suitable for various crops such as wheat, Shanghai Qing and peas, and has important application value in agricultural production in arid regions.

The invention provides acinetobacter for culturing prawns in saline-alkali water and application of the acinetobacter. The preservation number of the provided acinetobacter is CGMCC (China General Microbiological Culture Collection Center) No.36066. The acinetobacter soilis strain provided by the invention has remarkable comprehensive application advantages, can still keep higher growth activity and metabolic capability in a low-temperature environment, and continuously plays a water body purification function. Ammonia nitrogen and nitrite nitrogen in a water body can be efficiently degraded, the environment of the aquatic water body is remarkably improved by promoting the nitrogen circulation process, and harm caused by nitrogen pollution is reduced. In addition, the strain can effectively inhibit and prevent reproduction of pathogenic vibrios and reduce the occurrence risk of bacterial diseases of aquatic animals. The strain is high in safety, does not cause poison or stress reaction to bred animals even under the condition of high-concentration use, and has good ecological safety and environmental friendliness.

This invention relates to a 1,3,4-thiadiazole peptide deformylase inhibitor and its preparation and application. The structural formula is shown in formula (1): wherein, R 1 It is n-butyl or cyclopentylmethyl; R 2 The compounds are hydrogen-containing, straight-chain alkanes, cyclic alkanes, aromatic rings, substituted biphenyls, or heterocyclic rings. The 1,3,4-thiadiazole peptide deformylase inhibitors provided by this invention can effectively inhibit bacterial protein synthesis, thereby achieving sterilization. These compounds exhibit excellent inhibitory activity against Gram-positive drug-resistant bacteria, especially the clinically challenging methicillin-resistant Staphylococcus aureus (MRSA). In particular, some preferred compounds show inhibitory activity 4-8 times that of the control standards vancomycin and linezolid. The 1,3,4-thiadiazole peptide deformylase inhibitors provided by this invention also exhibit inhibitory activity against Gram-negative drug-resistant bacteria, especially against drug-resistant Acinetobacter baumannii, known as "superbugs," with an inhibitory activity reaching 0.5 μg / mL, far superior to vancomycin and linezolid.

The application discloses a malt PYF prediction method based on metagenome detection and belongs to the field of malt quality detection. Candida The relative abundance of Phytobacter The relative abundance of Curtobacterium The relative abundance of Kosakonia The relative abundance of Enterobacter The relative abundance of Fusarium The relative abundance of Aureobasidium The relative abundance of Acinetobacter The relative abundance of Klebsiella The relative abundance of The PYF potential is calculated, and the smaller the PYF potential is, the lower the degree of yeast pre-flocculation caused by the malt is. The application is applied to the yeast pre-flocculation degree prediction aspect, solves the problem that the existing malt PYF detection method cannot accurately reflect the yeast pre-flocculation condition in actual application, has the characteristics that the detection result is more accurate and can truly reflect the degree of yeast pre-flocculation caused by the malt.

The invention belongs to the technical field of medicines, and particularly relates to an aryl dicarboxylic acid compound, a preparation method and application of the aryl dicarboxylic acid compound as an antibacterial agent. The compound is shown as a formula (1), wherein R1, R2, R3, R4, R5, R6, R7, R8, R9 and R10 are independently selected from hydrogen and halogen. Two ends of the molecule are connected through an N-phenylpropanamide connecting arm, the molecule is a compound with a novel structure, and the series of compounds have a relatively strong inhibition effect on acinetobacter baumannii and are worthy of further research and development.

The invention relates to the technical field of bacteriophages, and particularly discloses an acinetobacter baumannii bacteriophage, depolymerases and a preparation method and application of the acinetobacter baumannii bacteriophage. The acinetobacter baumannii bacteriophage P919 provided by the invention has very strong specific lysis capability on KL3 type acinetobacter baumannii, and the coded depolymerases of the acinetobacter baumannii bacteriophage P919 have excellent temperature stability at 70 DEG C or below, and can effectively degrade capsular polysaccharide of the KL3 type acinetobacter baumannii.

The present invention relates to an antibacterial or antifungal composition and a pharmaceutical composition for preventing or treating bacterial or fungal infections, both comprising VLX600. The antibacterial composition comprising VLX600 according to one aspect of the present invention exhibits antibacterial or antifungal activity against a wide range of strains, including Mycobacterium spp., Escherichia spp., Pseudomonas spp., Staphylococcus spp., Acinetobacter spp., Candida spp., and the like. The pharmaceutical composition comprising VLX600 demonstrates excellent antibacterial, antifungal, and therapeutic efficacy against pathogens and can be effectively utilized for the prevention or treatment of bacterial or fungal infections. Furthermore, VLX600 exhibits a synergistic effect with conventional antibiotics and thus is effective in treating infections caused by antibiotic-resistant bacterial strains.

The invention discloses a composite microbial agent for degrading micro-plastics in seawater and a preparation method thereof, the microbial agent is composed of effective viable bacteria and auxiliary components, the effective viable bacteria are marine-derived halomonas, acinetobacter and bacillus and pseudomonas with high affinity with the micro-plastics, the effective viable bacteria are compounded according to a specific viable bacteria number ratio, and the microplastics are degraded by the auxiliary components. And function collaboration is realized. The auxiliary components comprise a protective agent consisting of trehalose, skimmed milk, glycerol and cane sugar, and a nutritional agent consisting of glucose and yeast extract. The preparation method comprises the following steps: respectively fermenting, centrifugally concentrating, compounding in proportion, mixing with auxiliary components, and finally drying to prepare powder. The invention further discloses application of the microbial inoculum to degradation of microplastics in beach intertidal zones and ship wastewater systems. The microbial agent is high in pertinence, good in stability in a high-salt seawater environment and high in degradation efficiency, and an effective solution is provided for in-situ bioremediation of marine micro-plastic pollution.

The invention discloses a strain of lactobacillus muricatum and application thereof.The lactobacillus muricatum SZH025 obtained through the method has an obvious inhibiting effect on acinetobacter baumannii, klebsiella pneumoniae and escherichia coli under the in-vitro condition and is suitable for preventing and treating intestinal infectious diseases or adjusting intestinal flora; the cholate hydrolase (BSH) activity is remarkable, bile acid metabolism is facilitated, cholesterol excretion is promoted, and the blood fat reducing and regulating effects are achieved. In addition, the strain shows good safety characteristics, has relatively strong gastrointestinal tract environmental adaptability, has certain antioxidant function and ethanol decomposition capacity, is beneficial to alcohol metabolism, can produce oleic acid, and has potential probiotic effects of regulating lipid, resisting inflammation and improving the metabolic state of a host.

The present invention relates to a composition that is used to primarily disrupt and remove biofilm from surfaces. This is achieved using a composition comprising a bacteriophage capable of lysing bacteria selected from one or more of Pseudomonas aeruginosa, Acinetobacter baumanii, Staphylococcus aureus, and E.coli; and a Bacillus bacterial spore in the presence of a specific surfactant of the poloxamer type.

The invention discloses a low-temperature denitrification strain and application of the low-temperature denitrification strain. The strain is Acinetobacter Bragg FX1, and the preservation number of the strain is CGMCC (China General Microbiological Culture Collection Center) NO: 36583. The strain can efficiently remove ammonia nitrogen, nitrate nitrogen and nitrite nitrogen in water at the temperature of 5-30 DEG C, especially shows the optimal denitrification performance at the temperature of about 10 DEG C, and the removal rate of the ammonia nitrogen and the nitrite nitrogen can reach 99% or above. The strain has strong adaptability to low-temperature environment, can tolerate water quality fluctuation of actual sewage, and solves the problems of low denitrification efficiency and unstable operation caused by insufficient microbial activity of sewage treatment plants in low-temperature regions. The strain can be used for preparing a low-temperature sewage denitrification microbial agent, can be directly added into a low-temperature domestic sewage or industrial wastewater treatment system, does not need to depend on high-energy-consumption heating and heat preservation measures, and has remarkable energy-saving and consumption-reducing potential, good environmental adaptability and wide application prospects.

The invention relates to the technical field of biological detection, in particular to a nucleic acid combination product, a PCR premix, a method and a kit for identifying respiratory tract pathogenic bacteria. The nucleic acid combination product comprises a primer pair as shown in SEQ ID NO: 1 to SEQ ID NO: 21 and a probe. The invention provides a nucleic acid detection product for detecting klebsiella pneumoniae, streptococcus pneumoniae, haemophilus influenzae, pseudomonas aeruginosa, acinetobacter baumannii, staphylococcus aureus and moraxella catarrhalis, and the nucleic acid detection product can be used for performing amplification detection on conserved fragments of target pathogens in the same PCR reaction system, and is efficient and low in cost. Moreover, the anti-interference capability is strong, and the sensitivity is high.

The application discloses a chimeric endolysin Lys009-Lys009CBD and engineering and application thereof, and belongs to the field of genetic engineering and protein engineering. The application constructs a chimeric endolysin Lys009-Lys009CBD with high activity, and the peptidoglycan hydrolysis activity of the chimeric endolysin Lys009-Lys009CBD on Pseudomonas aeruginosa is 5.30 times that of natural endolysin Lys009 and 27.98 times that of hen egg white lysozyme HEWL. A transmembrane peptide P81417 is further fused to perform transmembrane modification, and an engineering endolysin P81417-Lys009-Lys009CBD is obtained. The engineering endolysin can efficiently lyse Pseudomonas aeruginosa, Acinetobacter baumannii and the like, and has excellent thermal stability. The application enriches the resource library of high-activity endolysins, provides important experimental basis and technical support for developing an anti-drug-resistant bacteria treatment strategy, and also provides a new selection for clinical treatment of multiple drug-resistant bacteria infection.

The invention discloses application of chlorpromazine hydrochloride in preparation of an acinetobacter baumannii infection resisting medicine, and belongs to the technical field of biological medicine. The invention proves that when the chlorpromazine hydrochloride is independently used, the growth of the acinetobacter baumannii can be inhibited, wherein the acinetobacter baumannii comprises colistin-resistant acinetobacter baumannii; meanwhile, chlorpromazine hydrochloride can cooperate with colistin to play a role in resisting acinetobacter baumannii, so that infection caused by colistin-resistant acinetobacter baumannii is treated. When a certain concentration of chlorpromazine hydrochloride and colistin are combined for use, more than 50% of colistin-resistant acinetobacter baumannii is killed within 4 hours, and the survival rate is lower than 1%, which proves that chlorpromazine hydrochloride and colistin have a synergistic effect. The chlorpromazine hydrochloride and the colistin are combined for use, so that the chlorpromazine hydrochloride and the colistin can be used for resisting colistin drug-resistant pathogens and treating diseases caused by colistin drug-resistant bacteria infection, and meanwhile, the evolution of colistin drug resistance can also be prevented.

The application discloses a strain of Acinetobacter, the strain name is C16ZT1, and simultaneously proposes a bacterial agent containing the different bacillus, and further uses the bacterial agent for treating petroleum pollution wastewater. The Acinetobacter can efficiently degrade the petroleum production wastewater containing chain alkanes, can still realize the degradation effect quickly and stably under the conditions of high concentration, high salt and extreme acid and alkali, and can degrade more than 95% of the petroleum production wastewater within 144 hours. In the application in sewage treatment, the strain has strong tolerance, can tolerate high-concentration alkanes, and has a wide adaptation range to salt concentration, pH and the like, and has a good application prospect.

This invention relates to the fields of environmental microbiology and wastewater treatment technology, specifically to a highly salt-tolerant Acinetobacter baumannii strain for heterotrophic nitrification and aerobic denitrification of wastewater, a microbial agent, and its application. The strain is Acinetobacter baumannii. Acinetobacter baumannii M4, deposited on May 12, 2025, at the Guangdong Provincial Microbial Culture Collection Center, located at 5th Floor, Building 59, No. 100 Xianlie Middle Road, Yuexiu District, Guangzhou, Guangdong Province, with accession number GDMCC No: 66302. This invention utilizes the *Acinetobacter baumannii* strain M4, domesticated using a multi-round salt-beating method. It can grow in high-salt-alkali environments and exhibits both heterotrophic nitrification and aerobic denitrification capabilities. It can be widely applied to the biological denitrification and phosphorus removal treatment of high-salt-alkali nitrogen and phosphorus wastewater, including wastewater from aquaculture, pickling, and industrial processes.

The invention belongs to the field of microorganisms, and discloses an ammonia nitrogen conversion strain and application thereof, a strain D-5 with an ammonia nitrogen conversion effect provided by the invention is Acinetobacter seohaensis, and the strain D-5 can directly convert ammonia nitrogen into hydroxylamine, nitrite and nitrate. The strain is cultured in swine wastewater (liquid part after solid-liquid separation) with high-concentration nitrogen, phosphorus and organic carbon, the growth condition of the strain is good, the ammonia nitrogen conversion effect is remarkable, and the ammonia nitrogen conversion rate reaches 86.92%.

The invention belongs to the technical field of bioengineering, and particularly relates to a phage cocktail and application thereof. The bacteriophage cocktail comprises five bacteriophages PGPA20, PGPA60, PAB26, Lkpp-25 and Lkpp-36 which are separated from the natural world, and the bacteriophage cocktail has relatively strong bactericidal activity on pseudomonas aeruginosa, acinetobacter baumannii and K1 / K2 type klebsiella pneumoniae, and has specificity on the pseudomonas aeruginosa, the acinetobacter baumannii and the K1 / K2 type klebsiella pneumoniae; the bacteriophage can be compounded with other substances for use, and a safe and non-toxic bacteriophage killing product is provided for in-vivo and in-vitro infection treatment of pseudomonas aeruginosa, acinetobacter baumannii and K1 / K2 type klebsiella pneumoniae and disinfection and purification of pseudomonas aeruginosa, acinetobacter baumannii and K1 / K2 type klebsiella pneumoniae in the breeding industry and other environments.

The application discloses a liposome vaccine of Acinetobacter baumannii outer membrane protein and a preparation method and application thereof, and relates to the field of biological medicines. The application efficiently expresses the Acinetobacter baumannii outer membrane protein through a genetic engineering method, and obtains a liposome vaccine of Acinetobacter baumannii outer membrane protein by combining the obtained various outer membrane proteins as antigens with a lipid mixture and an adjuvant. It is verified that the vaccine can provide complete protection for mice attacked by a lethal dose of Acinetobacter baumannii. In the application, the outer membrane protein loaded in the liposome is re-folded in structure, the immunogenicity of the protein is enhanced, and the liposome can reduce the toxicity of the outer membrane protein. Omp38+BauA+FimA can be an effective antigen combination for preventing Acinetobacter baumannii infection. Pam2CSK4 as a TLR2 agonist has a strong auxiliary effect on the liposome vaccine of Acinetobacter baumannii, and can induce the activation of an innate immune response, thereby providing more comprehensive protection for a host.

The application relates to the technical field of biological medicine, and particularly discloses application of indole-3-methanol in preparation of polymyxin antibacterial synergist and a gram-negative bacterium-resistant pharmaceutical composition, the application of the indole-3-methanol in preparation of the polymyxin antibacterial synergist, the concentration of the polymyxin and the indole-3-methanol is 0.01-2 (mu g / mL):0.01-10 mM; the gram-negative bacterium-resistant pharmaceutical composition comprises the polymyxin and the indole-3-methanol, and further comprises pharmaceutically acceptable adjuvants and / or carriers; the composition can be prepared into injections, ointments or aerosols, and can be used for treating klebsiella pneumoniae, bauman acinetobacter, escherichia coli or pseudomonas aeruginosa and the like; the application discloses that the indole-3-methanol can synergize with the polymyxin, significantly improves the bactericidal activity of the polymyxin on gram-negative bacteria, reduces the use dosage of the polymyxin, improves the antibacterial efficacy, prolongs the antibacterial time, and delays the generation of drug resistance.

The application discloses a kind of microbial complex bacterial population, microbial inoculum and application thereof, and is related to microorganism and application technical field.The microbial complex bacterial population includes Acinetobacter sichuanensis LHH12, Pseudomonas songnenensis SJ2-76, Diaphorobacter nitroreducens SYY4 and Thauera aminoaromatica SJ2-12.The microorganism for constructing complex bacterial population is obtained from semi-coke wastewater, and has good phenol tolerance and chemical oxygen demand (COD) degradation capacity, and can be used for purifying high-phenol wastewater, biological denitrification and phosphorus removal and degrading COD.The microbial complex bacterial population of the application can effectively improve the COD degradation rate in phenol ammonia wastewater and effectively reduce the treatment cost of high-phenol ammonia wastewater such as semi-coke without changing the original microbial community composition of wastewater through the synergistic effect between strains, which has great potential for promoting low-carbon green and sustainable development of coal chemical industry.

This invention belongs to the field of microbiology, specifically relating to a biofilm sensor and a method for detecting biotoxicity using it. The specific technical solution involves a bacterial preparation comprising *Geobacterium*, *Acinetobacter*, and *Pseudomonas*. A sensor is prepared using this bacterial preparation and used to detect the biotoxicity of pollutants. This invention provides a novel biofilm sensor that, based on the high electroactivity of *Geobacterium* and by limiting the amount of carbon source used during cultivation, ultimately achieves an electroactive biofilm with a thickness lower than that of existing technologies, and realizes extremely high detection sensitivity, possessing enormous potential for detection applications.

The invention relates to a novel bacterial strain capable of degrading pesticides, in particular to a novel bacterial strain Acinetobacter oryzae F9-1, and a preparation method of the novel bacterial strain Acinetobacter oryzae F9-1. A new strain Acinetobacter oryzae F9-1 is obtained by separation, screening and identification from a soil sample in Hami region of Xinjiang, the strain is subjected to physiological and biochemical characteristic detection, the new strain F9-1 shows that the bacterial colony is small, the surface is smooth, the new strain F9-1 is white and semitransparent on an NB plate, the results of Gram staining, catalase and indole experiments and the like are positive, and the results of oxidase, V.P tests, urease and the like are negative. The strain F9-1 can hydrolyze hydrogen peroxide; l-alanine, lactic acid, L-aspartic acid, quinic acid and the like can be utilized, the strain is sensitive to 8% NaCl, lincomycin, nalidixic acid, chloramphenicol and the like, fluridone and pendimethalin can be degraded, and the strain has the advantages of being environmentally friendly, efficient, economical, sustainable and the like and is an effective means for solving the pesticide pollution problem.

Compositions for identifying a pathogenic bacterial infection include a mannitol compound with one or more substituents including radioisotopes. These radiopharmaceuticals, such as a positron-emitting mannitol analogue, [18F]fluoromannitol ([18F]FMtl), are rapidly taken up by gram-positive and gram-negative bacteria such as E. coli, S. aureus, A. baumannii, S. epidermis, P. mirabilis, S. enterica, K. pneumonia, E. faecium, E. cloacae, and M. marinum, but not non-active infection sites such as sterile inflammatory sites, cancer sites, etc. Administration of these radiopharmaceuticals to and subsequent imaging of patients, e.g., via positron emission tomography (PET), enables detection of deep-seated and difficult to manage bacterial infections, such as osteomyelitis and prosthetic joint infection, or in patients with sickle cell disease. [18F]FMtl injection detects and differentiates infection rapidly. The radiolabeled mannitol compounds can be produced via nucleophilic substitution reactions that are deployable on commercially available synthesizers, facilitating straightforward and wide accessibility, and counteracting unnecessary antibiotic use.

The invention discloses a carbapenem drug resistance gene detection primer group based on multiple PCR-time-of-flight mass spectrometry, a detection kit and a kit using method, and through a large number of screening and verification experiments, a multiple primer group suitable for time-of-flight mass spectrometry detection is obtained. Therefore, the multi-PCR-time-of-flight mass spectrometry detection of the multi-drug-resistant genes of carbapenem-resistant enterobacteriaceae bacteria becomes possible. The detection kit prepared by adopting the primer group can be used for simultaneously detecting and identifying 10 common carbapenem drug-resistant genes, including klebsiella pneumoniae KPC gene, NDM gene, IMP gene, VIM gene and OXA gene, acinetobacter baumannii OXA gene, NDM gene, Escherichia coli OXA gene, IMP gene and serratia marcescens VIM gene. Meanwhile, the kit is high in sensitivity and specificity, dozens of times of detection can be carried out in the same batch, so that the detection efficiency is greatly improved, and the kit is suitable for rapid screening and genetic typing of carbapenem drug-resistant bacteria in clinical samples.

This invention discloses a specific primer, method, and application for detecting tet(X)-positive Acinetobacter ininducible bacteria. The primer consists of two pairs of primers: indicus-6F / indicus-6R and universe-AXF3 / universe-AXR3. The PCR amplification products show clear electrophoretic bands, and the sequencing results are accurate. The amplification product size of primers indicus-6F / indicus-6R is 313 bp, and the amplification product size of primers universe-AXF3 / universe-AXR3 is 729 bp. The optimal amplification annealing temperature is 50℃, the lowest detection limit for DNA is 0.012 ng / μL, and the detection accuracy based on both genomic DNA and bacterial culture is 100%. The total detection time using bacterial culture is 6 hours. This method for detecting tet(X)-positive Acinetobacter ininducible bacteria is convenient to operate, highly sensitive, highly accurate, and time-efficient, and has broad application prospects.

The application discloses a method for constructing an animal model of chronic pulmonary infection / persistence of Acinetobacter baumannii, and belongs to the technical field of infectious disease animal model construction and microbial preparation. Compared with the acute infection and rapid clearance mode formed by directly inoculating free bacteria, the live bacterial microspheres prepared in the application can prolong the local exposure and detectable time of Acinetobacter baumannii in the lung, so that the Acinetobacter baumannii is more likely to form a low-load, long-term persistence pulmonary infection state, thereby providing a more stable experimental platform for the mechanism of chronic infection formation, host immune dynamic change and related intervention evaluation.

This invention relates to a petroleum hydrocarbon-degrading bacterium and its application, belonging to the field of microbial technology. To address the technical problems in existing petroleum-contaminated soil bioremediation, such as the scarcity of highly efficient degrading strains, low degradation efficiency, and insufficient capacity to handle complex hydrocarbons, this invention provides a petroleum hydrocarbon-degrading bacterium, *Acinetobacter pylori* AFRC34, with accession number CCTCC No:2026007. This strain can use petroleum hydrocarbon organic matter in diesel fuel as the sole carbon source and grows on inorganic salt culture media. It maintains a high petroleum hydrocarbon degradation rate even at a low temperature of 15℃. In an inorganic salt culture medium containing 10,000 mg / L high-concentration diesel fuel at 37℃, the degradation rate reaches 92.46%. The strain and inoculant provided by this invention can efficiently degrade petroleum hydrocarbon pollutants, and are particularly suitable for in-situ bioremediation in cold regions and heavily contaminated sites.

This invention discloses a kit for constructing an iron-dependent growth environment for Acinetobacter baumannii and its application method, belonging to the field of biotechnology. The kit is specifically designed for Acinetobacter baumannii, and its core components include a 50 mmol / L sterile stock solution tube of 2,2′-bipyridine, a 2000 μmol / L sterile stock solution tube of FeCl3, and a user manual. It does not include general-purpose LB medium, thus avoiding the problem of high-temperature and high-pressure deterioration of core reagents and highlighting the innovative combination of dedicated reagents. This invention also provides the application method of the kit. By adding the kit stock solution to the autoclaved and cooled LB medium in a specific ratio, standardized growth environments with normal iron, iron deficiency, and iron enrichment can be rapidly constructed. Dynamic monitoring and verification of the reversibility of growth inhibition-recovery ensure that the iron environment is precise and controllable. The kit is easy to operate, has good reproducibility, and can achieve standardized construction of the iron environment for Acinetobacter baumannii. It is suitable for iron metabolism-related research such as functional analysis of iron uptake-related proteins, development of iron deprivation antibacterial strategies, and screening of iron-deficient strains.