208 results about "Acinetobacter" patented technology

The invention relates to the field of microorganisms, in particular to a plant lactobacillus and application thereof in bacteriostasis. Specifically, the preservation number of the plant lactobacillus LP06 is CGMCC (China General Microbiological Culture Collection Center) No. 28412. The plant lactobacillus LP06 provided by the invention can be used for inhibiting the growth of debaryomyces hansenii, candida tropicalis and penicillium rochei, and also has an inhibiting effect on super bacteria acinetobacter baumannii, pseudomonas aeruginosa, klebsiella, escherichia coli, staphylococcus aureus and listeria monocytogenes. The plant lactobacillus LP06 provided by the invention has broad-spectrum bacteriostatic ability, and bacteriostatic powder prepared from the bacterium can be used as a biological preservative for corrosion prevention of dairy products such as yoghourt, cheese and the like and other foods.

The invention provides an antibacterial peptide cAMP048 sourced from microorganisms in a deep sea environment and application of the antibacterial peptide cAMP048, and belongs to the technical field of peptide inhibitors. The antibacterial peptide cAMP048 has an amino acid sequence as shown in SEQ ID NO: 1 (Sequence Identity Number 1). The antibacterial peptide can inhibit the growth of acinetobacter baumannii at 64 mu M, inhibit the growth of multi-drug-resistant acinetobacter baumannii and multi-drug-resistant enterococcus faecium at 128 mu M and inhibit the growth of escherichia coli, staphylococcus aureus and multi-drug-resistant klebsiella pneumoniae at 256 mu M. The antibacterial peptide can be used for preparing products with antibacterial and / or bactericidal performance, and can be applied to preparation of antibacterial and / or bactericidal products. Such as food, skin care products or medical supplies.

The invention discloses an antibacterial peptide and application thereof, and belongs to the technical field of biology. The antibacterial peptide with an inhibition effect on various clinically common pathogenic microorganisms is obtained, and the antibacterial peptide comprises gram-negative bacteria such as escherichia coli, klebsiella pneumoniae, pseudomonas aeruginosa, acinetobacter baumannii, vibrio parahaemolyticus, salmonella gallinarum, salmonella typhimurium and enterobacter sakazakii, and gram-positive bacteria. The antibacterial peptide provided by the invention can be used as an antibacterial peptide, such as staphylococcus aureus, listeria monocytogenes and bacillus cereus, and the antibacterial peptide provided by the invention is novel in structure and easy to prepare.

The invention discloses alkali-producing cadmium-tolerant acinetobacter capable of increasing the pH value of soil and application of the alkali-producing cadmium-tolerant acinetobacter, and belongs to the technical field of microorganisms, the alkali-producing cadmium-tolerant acinetobacter is preserved in the China General Microbiological Culture Collection Center (CGMCC), the number of the alkali-producing cadmium-tolerant acinetobacter is CGMCC No.33619, and the alkali-producing cadmium-tolerant acinetobacter belongs to proteobacteria and pseudomonas. The strain can increase the environmental pH value from 6.0 to 8.0 through metabolism, and stably grows in an environment with the cadmium (Cd < 2 + >) concentration as high as 20 ppm. The strain can be applied to the fields of acid soil improvement, cadmium pollution remediation, industrial wastewater treatment, fermentation engineering and the like. The method has the advantages of being environmentally friendly, efficient, stable, applicable to multiple scenes and the like, and a novel microbial resource is provided for heavy metal pollution treatment and soil ecological restoration.

The invention discloses a complex microbial inoculant for treating underground water as well as a preparation method and application thereof, relates to the field of sewage treatment, and aims to solve the problem that TBBPA and nitrate in underground water are difficult to remove at the same time in an existing biological treatment method. The composite microbial agent is prepared from pseudomonas koreensis Y5-11, acinetobacter pidermatum LYS-5, Pseudomonas sp. Y39-6 and psychrophilic bacteria F5-6, and the pseudomonas sp. Y5-11, the acinetobacter pidermatum LYS-5, the Pseudomonas sp. Y39-6, the psychrophilic bacteria F5-6 and the psychrophilic bacteria F5-6 are added into the microbial agent. The four strains are respectively subjected to fermentation culture, and the four fermentation liquids are mixed to prepare the complex microbial inoculant. The four bacteria in the complex microbial inoculant have a stronger metabolism complementation effect, and the effect on composite pollution remediation is more remarkable. The complex microbial inoculant is used for removing TBBPA and nitrate in underground water at the same time, the complex microbial inoculant has biodiversity, and the treatment result conforms to the water quality characteristics of the underground water.

The invention discloses a urinary system infection pathogen and drug resistance gene detection system and a kit. The detection system comprises forward and reverse primers which are respectively used for detecting escherichia coli, staphylococcus aureus, acinetobacter baumannii, klebsiella pneumoniae, enterococcus faecium, candida glabrata, proteus mirabilis, enterococcus faecalis, candida tropicalis, enterobacter cloacae, candida albicans, pseudomonas aeruginosa and streptococcus agalactiae; the forward primer and the reverse primer are respectively used for detecting the blaCTX-M gene of the extended spectrum beta-lactamase, the forward primer and the reverse primer are used for detecting the blaSHV gene of the extended spectrum beta-lactamase, and the forward primer and the reverse primer are used for detecting the blaTEM gene of the extended spectrum beta-lactamase. According to the forward and reverse primers for detecting the blaNDM gene of the extended-spectrum beta-lactamase, a detection sample is urine. According to the invention, common gram-negative bacteria, gram-positive bacteria and fungi can be detected at the same time, and the problem of missed detection of mixed infection in traditional culture is solved.

The invention discloses a polypeptide derived from deep sea microorganisms, and belongs to the technical field of peptide inhibitors. The polypeptide has an amino acid sequence as shown in SEQ ID NO: 1; the polypeptide has a bacteriostatic and / or bactericidal function. The polypeptide can inhibit the growth of acinetobacter baumannii at 8 [mu] M and inhibit the growth of escherichia coli and multi-drug-resistant acinetobacter baumannii at 16 [mu] M, and can be used for preparing products with bacteriostatic and / or bactericidal performance, such as food, daily necessities or medical products.

The invention belongs to the technical field of antibacterial peptides, and particularly relates to an LL-37 derived antibacterial peptide and application thereof. According to the invention, 17-29aa of the LL-37 antibacterial peptide is used as a core fragment and is connected with an amino acid sequence shown as SEQ ID NO: 1 or SEQ ID NO: 2 through an amido bond, the antibacterial activity of the obtained antibacterial peptide is obviously higher than that of the LL-37 antibacterial peptide, and the antibacterial peptide shows an obvious bactericidal effect on clinical drug-resistant bacteria such as acinetobacter baumannii, pseudomonas aeruginosa, escherichia coli, staphylococcus aureus, klebsiella pneumoniae and the like; meanwhile, the cytotoxicity and hemolytic activity are weak, drug resistance is not prone to being generated, bacteria can be rapidly killed in vivo through multiple mechanisms, no obvious cytotoxicity exists, the bacterium load in tissue is remarkably reduced, and the lifetime of a bacterium-infected mouse is prolonged.

The invention discloses a microbial composite flora, a microbial agent and application of the microbial composite flora and the microbial agent, and relates to the technical field of microorganisms and application of the microorganisms. The invention relates to a microbial composite flora, which is characterized in that the microbial composite flora comprises Acinetobacter sichuanensis LHH12, Pseudomonas sichuanensis SJ2-76, beneficial nitroreductase (Diaphorobacter nitroreducens SYY4, and Thauera ammoniaca SJ2-12, and the microbial composite flora comprises Acinetobacter sichuanensis LHH12, Pseudomonas sichuanensis SJ2-76, beneficial nitroreductase (Diaphorobacter nitroreducens SYY4, and Thauera ammoniaca SJ2-12. The microorganisms for constructing the composite flora are separated from the semi-coke wastewater, have better phenol resistance and chemical oxygen demand (COD) degradation capacity, and can be used for purifying high-phenol wastewater, biologically removing nitrogen and phosphorus and degrading COD. Under the condition that the original microbial community composition in the wastewater is not changed, the microbial compound flora can effectively improve the degradation rate of COD (Chemical Oxygen Demand) in the phenol-ammonia wastewater and effectively reduce the treatment cost of high-phenol-ammonia wastewater such as semi-coke and the like through the synergistic effect of the strains, and has great potential in the aspect of promoting the low-carbon green sustainable development of the coal chemical industry.

The invention discloses a phycomycete composite biological fertilizer for an arid loess area as well as a preparation method and application of the phycomycete composite biological fertilizer, and belongs to the technical field of biological fertilizers. The phycomycete composite biological fertilizer is prepared from nitrogen-fixing anabaena and acinetobacter, counted by a single cell, the total viable count of the anabaena azotica is greater than or equal to 1.0 * 10 < 6 > CFU / mL, and the total viable count of the acinetobacter is greater than or equal to 1.0 * 10 < 7 > CFU / mL. Experiments prove that the phycomycete composite biological fertilizer can significantly improve the germination potential and germination rate of crop seeds, promote seedling growth, improve the nutritional quality of crops and reduce the death rate of seedlings, is suitable for various crops such as wheat, Shanghai Qing and peas, and has important application value in agricultural production in arid regions.

The invention discloses application of jointly inoculated rhizosphere phosphate solubilizing bacteria in plant growth promotion and soil nutrient improvement, and belongs to the technical field of microorganisms. The acinetobacter and pantoea combined inoculation provided by the invention can effectively convert insoluble inorganic phosphorus into soluble inorganic phosphorus, thereby promoting the utilization of phosphorus. The ratio of acinetobacter to pantoea combined inoculation is (4-6): (6-4), the soil phosphorus dissolving capacity and the plant growth promoting effect are remarkable, and the compound fertilizer can be applied to improving the soil phosphorus utilization rate and promoting plant growth and has important agricultural and environmental values.

The invention relates to an Acinetobacter variants L49. The Acinetobacter variants L49 can rapidly and stably grow by utilizing a cheap culture medium, the fermentation period is short, and the lignin degradation efficiency is high. The strain can be used for degrading straws by a biological method, improves the utilization rate of the straws, and has very high economic value and ecological value.

The invention discloses an acinetobacter LYS-5 for degrading tetrabromobisphenol A and application thereof, relates to the field of microorganisms, and aims to solve the problem that tetrabromobisphenol A pollution in saline-alkali soil is difficult to repair by existing microorganisms. The acinetobacter is Acinetobacter pitefer LYS-5, and is preserved in China General Microbiological Culture Collection Center (CGMCC), the preservation address is No.3, No.1 yard, Beichen West Road, Chaoyang District, Beijing, the preservation date is April 22, 2024, and the preservation number is CGMCC No.30399. The invention further discloses a preparation method of the Acinetobacter pitefer LYS-5, and the acinetobacter pitefer LYS-5 has the advantages that the acinetobacter pitefer LYS-5 can be used for preparing the acinetobacter pitefer LYS-5; the method is used for degrading the pollutant tetrabromobisphenol A in a water body and saline-alkali soil.

The invention provides acinetobacter for culturing prawns in saline-alkali water and application of the acinetobacter. The preservation number of the provided acinetobacter is CGMCC (China General Microbiological Culture Collection Center) No.36066. The acinetobacter soilis strain provided by the invention has remarkable comprehensive application advantages, can still keep higher growth activity and metabolic capability in a low-temperature environment, and continuously plays a water body purification function. Ammonia nitrogen and nitrite nitrogen in a water body can be efficiently degraded, the environment of the aquatic water body is remarkably improved by promoting the nitrogen circulation process, and harm caused by nitrogen pollution is reduced. In addition, the strain can effectively inhibit and prevent reproduction of pathogenic vibrios and reduce the occurrence risk of bacterial diseases of aquatic animals. The strain is high in safety, does not cause poison or stress reaction to bred animals even under the condition of high-concentration use, and has good ecological safety and environmental friendliness.

The present invention relates to the pharmaceutical field, and provides a boric acid β-lactamase inhibitor or a use thereof. The boric acid β-lactamase inhibitor is a compound shown in formula (I), or an optical isomer or a pharmaceutically acceptable salt thereof: formula (I). The ultra-broad-spectrum boric acid β-lactamase inhibitor of the present invention has an inhibition effect on A, B, C, and D enzymes, and carbapenem drug-resistant bacteria such as Klebsiella pneumoniae, Escherichia coli, Enterobacter cloacae, Acinetobacter baumannii, and Pseudomonas aeruginosa.

This invention relates to a 1,3,4-thiadiazole peptide deformylase inhibitor and its preparation and application. The structural formula is shown in formula (1): wherein, R 1 It is n-butyl or cyclopentylmethyl; R 2 The compounds are hydrogen-containing, straight-chain alkanes, cyclic alkanes, aromatic rings, substituted biphenyls, or heterocyclic rings. The 1,3,4-thiadiazole peptide deformylase inhibitors provided by this invention can effectively inhibit bacterial protein synthesis, thereby achieving sterilization. These compounds exhibit excellent inhibitory activity against Gram-positive drug-resistant bacteria, especially the clinically challenging methicillin-resistant Staphylococcus aureus (MRSA). In particular, some preferred compounds show inhibitory activity 4-8 times that of the control standards vancomycin and linezolid. The 1,3,4-thiadiazole peptide deformylase inhibitors provided by this invention also exhibit inhibitory activity against Gram-negative drug-resistant bacteria, especially against drug-resistant Acinetobacter baumannii, known as "superbugs," with an inhibitory activity reaching 0.5 μg / mL, far superior to vancomycin and linezolid.

The application discloses a malt PYF prediction method based on metagenome detection and belongs to the field of malt quality detection. Candida The relative abundance of Phytobacter The relative abundance of Curtobacterium The relative abundance of Kosakonia The relative abundance of Enterobacter The relative abundance of Fusarium The relative abundance of Aureobasidium The relative abundance of Acinetobacter The relative abundance of Klebsiella The relative abundance of The PYF potential is calculated, and the smaller the PYF potential is, the lower the degree of yeast pre-flocculation caused by the malt is. The application is applied to the yeast pre-flocculation degree prediction aspect, solves the problem that the existing malt PYF detection method cannot accurately reflect the yeast pre-flocculation condition in actual application, has the characteristics that the detection result is more accurate and can truly reflect the degree of yeast pre-flocculation caused by the malt.

The invention belongs to the technical field of medicines, and particularly relates to an aryl dicarboxylic acid compound, a preparation method and application of the aryl dicarboxylic acid compound as an antibacterial agent. The compound is shown as a formula (1), wherein R1, R2, R3, R4, R5, R6, R7, R8, R9 and R10 are independently selected from hydrogen and halogen. Two ends of the molecule are connected through an N-phenylpropanamide connecting arm, the molecule is a compound with a novel structure, and the series of compounds have a relatively strong inhibition effect on acinetobacter baumannii and are worthy of further research and development.

The invention relates to the technical field of bacteriophages, and particularly discloses an acinetobacter baumannii bacteriophage, depolymerases and a preparation method and application of the acinetobacter baumannii bacteriophage. The acinetobacter baumannii bacteriophage P919 provided by the invention has very strong specific lysis capability on KL3 type acinetobacter baumannii, and the coded depolymerases of the acinetobacter baumannii bacteriophage P919 have excellent temperature stability at 70 DEG C or below, and can effectively degrade capsular polysaccharide of the KL3 type acinetobacter baumannii.

The invention relates to a biomarker for individual recognition and application thereof, and relates to the technical field of biology. The biomarker comprises at least one of the following microorganisms: propionibacterium acnes, staphylococcus epidermidis, marine paracoccus, streptococcus, rhodococcus fence celebrating, staphylococcus, pseudomonas, streptococcus sanguineus, achromobacter xylosoxidans, acinetobacter calcoaceticus, acinetobacter johnsonii, staphylococcus lentus, pseudomonas toralaris and brevundimonas vesicularis. Or enterococcus faecium. The biomarker can provide analysis with higher resolution in the aspect of differential strain information, has a better distinguishing effect, higher accuracy and sensitivity on individual recognition, and can be used as a new technical means for supplementing forensic individual recognition.

The present invention relates to an antibacterial or antifungal composition and a pharmaceutical composition for preventing or treating bacterial or fungal infections, both comprising VLX600. The antibacterial composition comprising VLX600 according to one aspect of the present invention exhibits antibacterial or antifungal activity against a wide range of strains, including Mycobacterium spp., Escherichia spp., Pseudomonas spp., Staphylococcus spp., Acinetobacter spp., Candida spp., and the like. The pharmaceutical composition comprising VLX600 demonstrates excellent antibacterial, antifungal, and therapeutic efficacy against pathogens and can be effectively utilized for the prevention or treatment of bacterial or fungal infections. Furthermore, VLX600 exhibits a synergistic effect with conventional antibiotics and thus is effective in treating infections caused by antibiotic-resistant bacterial strains.

The invention discloses a composite microbial agent for degrading micro-plastics in seawater and a preparation method thereof, the microbial agent is composed of effective viable bacteria and auxiliary components, the effective viable bacteria are marine-derived halomonas, acinetobacter and bacillus and pseudomonas with high affinity with the micro-plastics, the effective viable bacteria are compounded according to a specific viable bacteria number ratio, and the microplastics are degraded by the auxiliary components. And function collaboration is realized. The auxiliary components comprise a protective agent consisting of trehalose, skimmed milk, glycerol and cane sugar, and a nutritional agent consisting of glucose and yeast extract. The preparation method comprises the following steps: respectively fermenting, centrifugally concentrating, compounding in proportion, mixing with auxiliary components, and finally drying to prepare powder. The invention further discloses application of the microbial inoculum to degradation of microplastics in beach intertidal zones and ship wastewater systems. The microbial agent is high in pertinence, good in stability in a high-salt seawater environment and high in degradation efficiency, and an effective solution is provided for in-situ bioremediation of marine micro-plastic pollution.

The invention discloses a strain of lactobacillus muricatum and application thereof.The lactobacillus muricatum SZH025 obtained through the method has an obvious inhibiting effect on acinetobacter baumannii, klebsiella pneumoniae and escherichia coli under the in-vitro condition and is suitable for preventing and treating intestinal infectious diseases or adjusting intestinal flora; the cholate hydrolase (BSH) activity is remarkable, bile acid metabolism is facilitated, cholesterol excretion is promoted, and the blood fat reducing and regulating effects are achieved. In addition, the strain shows good safety characteristics, has relatively strong gastrointestinal tract environmental adaptability, has certain antioxidant function and ethanol decomposition capacity, is beneficial to alcohol metabolism, can produce oleic acid, and has potential probiotic effects of regulating lipid, resisting inflammation and improving the metabolic state of a host.

A strain Y26 for degrading lignin, characterized in that the strain Y26 is Acinetobacter baumannii (ATCC 19606T) Acinetobacter baumannii ), preserved in the China Center for Type Culture Collection, located at No. 299, Bajiyuan Road, Wuchang District, Wuhan City, Hubei Province, China, with a preservation number of CCTCCM20242944 and a preservation date of December 30, 2024. The Acinetobacter baumannii Y26 in the present application has excellent ability to degrade lignocellulose, with a Lac enzyme activity of 60.38±2.5 U / L, a Mnp enzyme activity of 2612.14±25 U / L, a Lip enzyme activity of 1460.62±14 U / L, and a lignin degradation rate in corn stalks of 38.93%. In the present application, the corn stalks are pretreated by using the Acinetobacter baumannii Y26 in combination with maleic acid, so that the cellulose conversion rate of the corn stalks reaches 87.41%, the glucose yield reaches 0.668 g / g of stalks, and the prepared saccharification liquid has small inhibition effect on microbial fermentation, effectively improving the ethanol yield.

The present invention relates to a composition that is used to primarily disrupt and remove biofilm from surfaces. This is achieved using a composition comprising a bacteriophage capable of lysing bacteria selected from one or more of Pseudomonas aeruginosa, Acinetobacter baumanii, Staphylococcus aureus, and E.coli; and a Bacillus bacterial spore in the presence of a specific surfactant of the poloxamer type.

The invention discloses a low-temperature denitrification strain and application of the low-temperature denitrification strain. The strain is Acinetobacter Bragg FX1, and the preservation number of the strain is CGMCC (China General Microbiological Culture Collection Center) NO: 36583. The strain can efficiently remove ammonia nitrogen, nitrate nitrogen and nitrite nitrogen in water at the temperature of 5-30 DEG C, especially shows the optimal denitrification performance at the temperature of about 10 DEG C, and the removal rate of the ammonia nitrogen and the nitrite nitrogen can reach 99% or above. The strain has strong adaptability to low-temperature environment, can tolerate water quality fluctuation of actual sewage, and solves the problems of low denitrification efficiency and unstable operation caused by insufficient microbial activity of sewage treatment plants in low-temperature regions. The strain can be used for preparing a low-temperature sewage denitrification microbial agent, can be directly added into a low-temperature domestic sewage or industrial wastewater treatment system, does not need to depend on high-energy-consumption heating and heat preservation measures, and has remarkable energy-saving and consumption-reducing potential, good environmental adaptability and wide application prospects.

The present invention belongs to the field of pharmaceutical technology and relates to a preparation that binds to the AbaR protein of Acinetobacter baumannii and its application. The present invention utilizes sansinol O to effectively bind to the AbaR protein in Acinetobacter baumannii, thereby inhibiting its AHL quorum sensing system. It has been confirmed that sansinol O can significantly inhibit the biofilm formation ability of Acinetobacter baumannii ATCC 19606 strain, reduce bacterial motility, and reduce bacterial toxicity to cells, without significantly affecting bacterial growth and causing selective pressure to promote bacterial evolution of drug resistance.

The invention discloses an acinetobacter baumannii phage-magnetic bead conjugate and a method for detecting bacteria, and belongs to the technical field of biomedicine. The conjugate is a compound obtained by coupling an acinetobacter baumannii phage vBAbaMB9 and a micron magnetic bead, and when the conjugate is used for bacterial detection, the acinetobacter baumannii can be rapidly and efficiently captured and enriched through the specific recognition of the phage and the adsorption effect of the micron magnetic bead, so that the specific recognition and rapid detection of the acinetobacter baumannii are realized. According to the method, the acinetobacter baumannii can be specifically enriched and separated, a bacteria detection result is directly observed under a confocal microscope, the time consumption is short, the sensitivity is high, the detection efficiency of the acinetobacter baumannii is greatly improved, the treatment of clinical bacterial infection is facilitated, and the method has very important significance on timely diagnosis and treatment of the acinetobacter baumannii.

The invention relates to the technical field of biological detection, in particular to a nucleic acid combination product, a PCR premix, a method and a kit for identifying respiratory tract pathogenic bacteria. The nucleic acid combination product comprises a primer pair as shown in SEQ ID NO: 1 to SEQ ID NO: 21 and a probe. The invention provides a nucleic acid detection product for detecting klebsiella pneumoniae, streptococcus pneumoniae, haemophilus influenzae, pseudomonas aeruginosa, acinetobacter baumannii, staphylococcus aureus and moraxella catarrhalis, and the nucleic acid detection product can be used for performing amplification detection on conserved fragments of target pathogens in the same PCR reaction system, and is efficient and low in cost. Moreover, the anti-interference capability is strong, and the sensitivity is high.

The invention discloses a soil conditioner for improving crop stress resistance, and relates to the technical field of soil conditioners. The invention relates to a soil conditioner for improving the stress resistance of crops, which is used for improving the stress resistance of the crops. Comprising the following raw materials in parts by weight: 70-80 parts of fermented nutrient elements, 10-20 parts of urea, 15-30 parts of diammonium phosphate, 10-20 parts of potassium chloride, 30-50 parts of a trace element compound fertilizer, 40-60 parts of a humic acid fertilizer, 1-10 parts of charcoal, 10-20 parts of gibberellin, 10-20 parts of chitin, 10-20 parts of bacillus megatherium, 10-20 parts of arthrobacter chrysoflavus and 1-5 parts of Sinorhizobium freudeneri. 1 to 5 parts of bacillus licheniformis and 1 to 5 parts of acinetobacter.