290 results about "Acinetobacter" patented technology

The invention relates to the technical field of antibacterial peptides, and particularly discloses a marine antibacterial peptide and application thereof, and the amino acid sequence of the marine antibacterial peptide is as shown in SEQ ID NO.1: MWKLPKWRNGRRRGFKIPRW. The marine antibacterial peptide CD109 provided by the invention has an inhibition effect on staphylococcus aureus, acinetobacter baumannii, pseudomonas aeruginosa and klebsiella pneumoniae, and has extremely low cytotoxicity.

The invention relates to the technical field of antibacterial peptide application, in particular to an antibacterial peptide for inhibiting drug-resistant acinetobacter baumannii as well as a preparation method and application of the antibacterial peptide. The antibacterial peptide is cd77AHP-2, and the amino acid sequence of the antibacterial peptide is shown as SEQ ID NO.1. The antibacterial peptide cd77 obtained through artificial chemical synthesis is modified, distribution of cationic amino acid is optimized, and the antibacterial activity of the antibacterial peptide is remarkably improved. The antibacterial peptide cd77AHP-2 has a remarkable antibacterial effect on acinetobacter baumannii which are gram-negative bacteria and multi-drug resistant acinetobacter baumannii, and the antibacterial peptide cd77AHP-2 has a remarkable antibacterial effect on the acinetobacter baumannii which are gram-negative bacteria. The antibacterial peptide cd77AHP-2 provided by the invention has relatively low cytotoxicity to human bronchial epithelial cells, and has the potential of preparing a drug for resisting drug-resistant acinetobacter baumannii. The invention provides a novel functional polypeptide for the field of antibacterial peptides, and the novel functional polypeptide has important practical application value.

The invention relates to the field of microorganisms, in particular to a plant lactobacillus and application thereof in bacteriostasis. Specifically, the preservation number of the plant lactobacillus LP06 is CGMCC (China General Microbiological Culture Collection Center) No. 28412. The plant lactobacillus LP06 provided by the invention can be used for inhibiting the growth of debaryomyces hansenii, candida tropicalis and penicillium rochei, and also has an inhibiting effect on super bacteria acinetobacter baumannii, pseudomonas aeruginosa, klebsiella, escherichia coli, staphylococcus aureus and listeria monocytogenes. The plant lactobacillus LP06 provided by the invention has broad-spectrum bacteriostatic ability, and bacteriostatic powder prepared from the bacterium can be used as a biological preservative for corrosion prevention of dairy products such as yoghourt, cheese and the like and other foods.

The invention provides an antibacterial peptide cAMP048 sourced from microorganisms in a deep sea environment and application of the antibacterial peptide cAMP048, and belongs to the technical field of peptide inhibitors. The antibacterial peptide cAMP048 has an amino acid sequence as shown in SEQ ID NO: 1 (Sequence Identity Number 1). The antibacterial peptide can inhibit the growth of acinetobacter baumannii at 64 mu M, inhibit the growth of multi-drug-resistant acinetobacter baumannii and multi-drug-resistant enterococcus faecium at 128 mu M and inhibit the growth of escherichia coli, staphylococcus aureus and multi-drug-resistant klebsiella pneumoniae at 256 mu M. The antibacterial peptide can be used for preparing products with antibacterial and / or bactericidal performance, and can be applied to preparation of antibacterial and / or bactericidal products. Such as food, skin care products or medical supplies.

The invention discloses an antibacterial peptide SAMP4 or a derivative thereof, a pharmaceutical composition and application, and belongs to the technical field of biology, the amino acid sequence of the antibacterial peptide SAMP4 is as shown in SEQ ID NO: 1, and the antibacterial peptide SAMP4 has a broad-spectrum antibacterial effect and can be used for preparing antibacterial peptide SAMP4. The compound has a strong bacteriostatic effect on various gram-positive bacteria such as staphylococcus aureus, enterococcus and streptococcus and various gram-negative bacteria such as escherichia coli, acinetobacter baumannii and pseudomonas aeruginosa, and is strong in antibacterial activity, wide in antibacterial spectrum and low in toxicity. The invention also provides a derivative and a salt of the antibacterial peptide SAMP4 and application of the antibacterial peptide SAMP4 and the derivative and the salt of the antibacterial peptide SAMP4 to research, development and preparation of various antibacterial products or / and antibacterial drugs and treatment of bacterial infectious diseases, and the antibacterial peptide SAMP4 and the derivative and the salt of the antibacterial peptide SAMP4 are remarkable in advantages and wide in application prospect.

The invention discloses an antibacterial peptide and application thereof, and belongs to the technical field of biology. The antibacterial peptide with an inhibition effect on various clinically common pathogenic microorganisms is obtained, and the antibacterial peptide comprises gram-negative bacteria such as escherichia coli, klebsiella pneumoniae, pseudomonas aeruginosa, acinetobacter baumannii, vibrio parahaemolyticus, salmonella gallinarum, salmonella typhimurium and enterobacter sakazakii, and gram-positive bacteria. The antibacterial peptide provided by the invention can be used as an antibacterial peptide, such as staphylococcus aureus, listeria monocytogenes and bacillus cereus, and the antibacterial peptide provided by the invention is novel in structure and easy to prepare.

The invention discloses alkali-producing cadmium-tolerant acinetobacter capable of increasing the pH value of soil and application of the alkali-producing cadmium-tolerant acinetobacter, and belongs to the technical field of microorganisms, the alkali-producing cadmium-tolerant acinetobacter is preserved in the China General Microbiological Culture Collection Center (CGMCC), the number of the alkali-producing cadmium-tolerant acinetobacter is CGMCC No.33619, and the alkali-producing cadmium-tolerant acinetobacter belongs to proteobacteria and pseudomonas. The strain can increase the environmental pH value from 6.0 to 8.0 through metabolism, and stably grows in an environment with the cadmium (Cd < 2 + >) concentration as high as 20 ppm. The strain can be applied to the fields of acid soil improvement, cadmium pollution remediation, industrial wastewater treatment, fermentation engineering and the like. The method has the advantages of being environmentally friendly, efficient, stable, applicable to multiple scenes and the like, and a novel microbial resource is provided for heavy metal pollution treatment and soil ecological restoration.

The invention relates to application of ten antibacterial peptides in the antimicrobial field. The ten antibacterial peptides have strong antibacterial activity on multi-drug-resistant bacteria, including but not limited to gram-negative bacteria such as escherichia coli, pseudomonas aeruginosa, acinetobacter baumannii, klebsiella pneumoniae and the like, and gram-positive bacteria such as staphylococcus aureus, enterococcus faecalis and the like. Activity research proves that any one of the ten antibacterial peptides has a remarkable effect of inhibiting, killing or preventing microbial infection. Due to the strong antibacterial property of any antibacterial peptide, the antibacterial peptide has wide application potential in the aspects of preparing antibacterial drugs, preservatives, antibacterial coatings, antibacterial fibers, antibacterial packaging materials and the like. Due to the unique property of the antibacterial peptide in the aspect of antibacterial performance, a new direction is provided for the development of efficient and reliable antibacterial agents and antibacterial technologies and products in multiple fields.

The acinetobacter Guilin is preserved in the China Center for Type Culture Collection (CCTCC), the preservation date is October 17, 2024, and the preservation number of the strain is CCTCCNO: M 20242235. The acinetobacter Guilin has the advantages that the acinetobacter Guilin can be used for degrading PBAT / PLA plastics, and the acinetobacter Guilin can be used for degrading PBAT / PLA plastics; according to the invention, effective degradation of PBAT / PLA plastic can be realized.

The invention discloses a complex microbial inoculant for treating underground water as well as a preparation method and application thereof, relates to the field of sewage treatment, and aims to solve the problem that TBBPA and nitrate in underground water are difficult to remove at the same time in an existing biological treatment method. The composite microbial agent is prepared from pseudomonas koreensis Y5-11, acinetobacter pidermatum LYS-5, Pseudomonas sp. Y39-6 and psychrophilic bacteria F5-6, and the pseudomonas sp. Y5-11, the acinetobacter pidermatum LYS-5, the Pseudomonas sp. Y39-6, the psychrophilic bacteria F5-6 and the psychrophilic bacteria F5-6 are added into the microbial agent. The four strains are respectively subjected to fermentation culture, and the four fermentation liquids are mixed to prepare the complex microbial inoculant. The four bacteria in the complex microbial inoculant have a stronger metabolism complementation effect, and the effect on composite pollution remediation is more remarkable. The complex microbial inoculant is used for removing TBBPA and nitrate in underground water at the same time, the complex microbial inoculant has biodiversity, and the treatment result conforms to the water quality characteristics of the underground water.

The invention discloses a urinary system infection pathogen and drug resistance gene detection system and a kit. The detection system comprises forward and reverse primers which are respectively used for detecting escherichia coli, staphylococcus aureus, acinetobacter baumannii, klebsiella pneumoniae, enterococcus faecium, candida glabrata, proteus mirabilis, enterococcus faecalis, candida tropicalis, enterobacter cloacae, candida albicans, pseudomonas aeruginosa and streptococcus agalactiae; the forward primer and the reverse primer are respectively used for detecting the blaCTX-M gene of the extended spectrum beta-lactamase, the forward primer and the reverse primer are used for detecting the blaSHV gene of the extended spectrum beta-lactamase, and the forward primer and the reverse primer are used for detecting the blaTEM gene of the extended spectrum beta-lactamase. According to the forward and reverse primers for detecting the blaNDM gene of the extended-spectrum beta-lactamase, a detection sample is urine. According to the invention, common gram-negative bacteria, gram-positive bacteria and fungi can be detected at the same time, and the problem of missed detection of mixed infection in traditional culture is solved.

To provide a plastic-degrading microorganism.SOLUTION: In one aspect, the present disclosure provides a microorganism capable of degrading plastic, belonging to a genus selected from the group consisting of the genus Acinetobacter, the genus Achromobacter, the genus Pseudomonas, the genus Serratia, the genus Amycolatopsis, and the genus Bacillus. In one aspect, the plastic to be degraded comprises at least one selected from polypropylene (PP), polyethylene (PE), polyvinylidene chloride (PVDC), and ethylene-vinyl alcohol copolymer (EVOH).SELECTED DRAWING: None

The invention discloses a polypeptide derived from deep sea microorganisms, and belongs to the technical field of peptide inhibitors. The polypeptide has an amino acid sequence as shown in SEQ ID NO: 1; the polypeptide has a bacteriostatic and / or bactericidal function. The polypeptide can inhibit the growth of acinetobacter baumannii at 8 [mu] M and inhibit the growth of escherichia coli and multi-drug-resistant acinetobacter baumannii at 16 [mu] M, and can be used for preparing products with bacteriostatic and / or bactericidal performance, such as food, daily necessities or medical products.

The invention relates to the technical field of medicines, in particular to an antibacterial composition as well as a preparation method and application thereof. The invention provides an antibacterial composition. The antibacterial composition is prepared from the following components in parts by mass: 0.039 to 0.3125 part of SU3327 and 62.5 to 500 parts of citric acid. The combined use of the SU3327 and the citric acid can effectively kill and / or inhibit vibrio parahaemolyticus, escherichia coli, salmonella, acinetobacter baumannii, staphylococcus aureus, klebsiella pneumoniae and staphylococcus pseudointermediates, and the use amount of the SU3327 in the medicament for treating bacterial infection is greatly reduced; and the compound shows extremely high application value in the process of preparing medicines for treating bacterial infection.

The invention discloses an acinetobacter baumannii outer membrane protein liposome vaccine as well as a preparation method and application thereof, and relates to the field of biological medicines. The acinetobacter baumannii outer membrane protein liposome vaccine is obtained by efficiently expressing the acinetobacter baumannii outer membrane protein through a genetic engineering means and combining the obtained multiple outer membrane proteins as antigens with a lipid mixture and an adjuvant, and the acinetobacter baumannii outer membrane protein liposome vaccine is proved to be capable of providing complete protection for mice challenged by lethal dose acinetobacter baumannii. Wherein the outer membrane protein loaded by the lipidosome realizes structural refolding, the protein immunogenicity is enhanced, and the lipidosome can reduce the toxicity of the outer membrane protein; omp38 + BauA + FimA may be an effective antigen combination for preventing acinetobacter baumannii infection; as a TLR2 agonist, Pam2CSK4 has a strong auxiliary effect on an acinetobacter baumannii liposome vaccine, can induce innate immune response activation, and provides a more comprehensive protection effect on a host.

The invention belongs to the technical field of antibacterial peptides, and particularly relates to an LL-37 derived antibacterial peptide and application thereof. According to the invention, 17-29aa of the LL-37 antibacterial peptide is used as a core fragment and is connected with an amino acid sequence shown as SEQ ID NO: 1 or SEQ ID NO: 2 through an amido bond, the antibacterial activity of the obtained antibacterial peptide is obviously higher than that of the LL-37 antibacterial peptide, and the antibacterial peptide shows an obvious bactericidal effect on clinical drug-resistant bacteria such as acinetobacter baumannii, pseudomonas aeruginosa, escherichia coli, staphylococcus aureus, klebsiella pneumoniae and the like; meanwhile, the cytotoxicity and hemolytic activity are weak, drug resistance is not prone to being generated, bacteria can be rapidly killed in vivo through multiple mechanisms, no obvious cytotoxicity exists, the bacterium load in tissue is remarkably reduced, and the lifetime of a bacterium-infected mouse is prolonged.

The invention discloses a microbial composite flora, a microbial agent and application of the microbial composite flora and the microbial agent, and relates to the technical field of microorganisms and application of the microorganisms. The invention relates to a microbial composite flora, which is characterized in that the microbial composite flora comprises Acinetobacter sichuanensis LHH12, Pseudomonas sichuanensis SJ2-76, beneficial nitroreductase (Diaphorobacter nitroreducens SYY4, and Thauera ammoniaca SJ2-12, and the microbial composite flora comprises Acinetobacter sichuanensis LHH12, Pseudomonas sichuanensis SJ2-76, beneficial nitroreductase (Diaphorobacter nitroreducens SYY4, and Thauera ammoniaca SJ2-12. The microorganisms for constructing the composite flora are separated from the semi-coke wastewater, have better phenol resistance and chemical oxygen demand (COD) degradation capacity, and can be used for purifying high-phenol wastewater, biologically removing nitrogen and phosphorus and degrading COD. Under the condition that the original microbial community composition in the wastewater is not changed, the microbial compound flora can effectively improve the degradation rate of COD (Chemical Oxygen Demand) in the phenol-ammonia wastewater and effectively reduce the treatment cost of high-phenol-ammonia wastewater such as semi-coke and the like through the synergistic effect of the strains, and has great potential in the aspect of promoting the low-carbon green sustainable development of the coal chemical industry.

The invention discloses a phycomycete composite biological fertilizer for an arid loess area as well as a preparation method and application of the phycomycete composite biological fertilizer, and belongs to the technical field of biological fertilizers. The phycomycete composite biological fertilizer is prepared from nitrogen-fixing anabaena and acinetobacter, counted by a single cell, the total viable count of the anabaena azotica is greater than or equal to 1.0 * 10 < 6 > CFU / mL, and the total viable count of the acinetobacter is greater than or equal to 1.0 * 10 < 7 > CFU / mL. Experiments prove that the phycomycete composite biological fertilizer can significantly improve the germination potential and germination rate of crop seeds, promote seedling growth, improve the nutritional quality of crops and reduce the death rate of seedlings, is suitable for various crops such as wheat, Shanghai Qing and peas, and has important application value in agricultural production in arid regions.

The invention discloses application of jointly inoculated rhizosphere phosphate solubilizing bacteria in plant growth promotion and soil nutrient improvement, and belongs to the technical field of microorganisms. The acinetobacter and pantoea combined inoculation provided by the invention can effectively convert insoluble inorganic phosphorus into soluble inorganic phosphorus, thereby promoting the utilization of phosphorus. The ratio of acinetobacter to pantoea combined inoculation is (4-6): (6-4), the soil phosphorus dissolving capacity and the plant growth promoting effect are remarkable, and the compound fertilizer can be applied to improving the soil phosphorus utilization rate and promoting plant growth and has important agricultural and environmental values.

The invention belongs to the field of molecular biological detection, and provides a joint detection composition which is mainly used for detecting related pathogens by using a multiple fluorescent PCR (Polymerase Chain Reaction) analysis method. Therefore, at least four of the klebsiella aerogenes, the stenotrophomonas maltophilia, the streptococcus angina, the acinetobacter baumannii, the klebsiella pneumoniae and the klebsiella oxytoca can be simultaneously detected and distinguished in a single-tube reaction system. Meanwhile, the detection sensitivity of the composition is higher and reaches 500 copies / mL, and the detection is more accurate.

The invention relates to an Acinetobacter variants L49. The Acinetobacter variants L49 can rapidly and stably grow by utilizing a cheap culture medium, the fermentation period is short, and the lignin degradation efficiency is high. The strain can be used for degrading straws by a biological method, improves the utilization rate of the straws, and has very high economic value and ecological value.

The invention provides a multiplex qPCR (quantitative polymerase chain reaction) detection primer and probe composition for detecting four psychrophilic bacteria and enzymes thereof and application, and belongs to the technical field of biological detection. The invention develops two quadruple qPCR methods for housekeeping genes and enzyme coding genes of pseudomonas, acinetobacter, bacillus cereus and stenotrophomonas maltophilia, the method has good specificity and versatility, the primer and the probe both show a good linear relationship between a Ct value and a colony count in single and multiple qPCR, and the primer and the probe can be used for detecting the colony count of the pseudomonas, the acinetobacter, the bacillus cereus and the stenotrophomonas maltophilia and the enzyme coding genes of the pseudomonas, the acinetobacter, the bacillus cereus and the stenotrophomonas maltophilia. The detection limit of the housekeeping gene is 101 CFU / mL, and the detection limit of the enzyme coding gene is 102 CFU / mL. The detection method can be used for evaluating the content of psychrophilic bacteria in milk and dairy products, the detection time is shortened to 2.5 h from 25 h, and the method has the advantages of being efficient, rapid and easy to operate.

The invention discloses an antibacterial peptide and application thereof. The antibacterial peptide comprises one or more of an antibacterial peptide PFK with an amino acid sequence as shown in SEQ ID NO: 1, an antibacterial peptide PVK with an amino acid sequence as shown in SEQ ID NO: 2 and an antibacterial peptide PIK with an amino acid sequence as shown in SEQ ID NO: 3. The antibacterial peptides PFK, PVK and PIK disclosed by the invention have antibacterial effects on porphyromonas gingivalis, streptococcus mutans, acinetobacter baumannii, escherichia coli and staphylococcus aureus, and the antibacterial peptides PFK, PVK and PIK have relatively low hemolytic activity and relatively low cytotoxicity, so that the antibacterial peptides PFK, PVK and PIK can be used for preparing biological antibacterial preparations, anti-infection drugs or oral products.

The invention discloses an acinetobacter LYS-5 for degrading tetrabromobisphenol A and application thereof, relates to the field of microorganisms, and aims to solve the problem that tetrabromobisphenol A pollution in saline-alkali soil is difficult to repair by existing microorganisms. The acinetobacter is Acinetobacter pitefer LYS-5, and is preserved in China General Microbiological Culture Collection Center (CGMCC), the preservation address is No.3, No.1 yard, Beichen West Road, Chaoyang District, Beijing, the preservation date is April 22, 2024, and the preservation number is CGMCC No.30399. The invention further discloses a preparation method of the Acinetobacter pitefer LYS-5, and the acinetobacter pitefer LYS-5 has the advantages that the acinetobacter pitefer LYS-5 can be used for preparing the acinetobacter pitefer LYS-5; the method is used for degrading the pollutant tetrabromobisphenol A in a water body and saline-alkali soil.

The invention discloses a composition and application thereof in resisting multi-drug resistant acinetobacter baumannii, and belongs to the technical field of bacterial infection resistance. The invention provides a composition for resisting multi-drug resistance acinetobacter baumannii. The composition is prepared from emodin, aloe-emodin and rhein, the mass ratio of the emodin to the aloe-emodin to the rhein is 1: 1: 1. The invention also provides an application of the composition in preparation of an antibacterial preparation. According to the composition disclosed by the invention, the sensitivity of the multi-drug-resistant acinetobacter baumannii to polymyxin and carbapenem antibiotics can be recovered again, and the killing time of the polymyxin and carbapenem antibiotics to the multi-drug-resistant acinetobacter baumannii can be shortened; the healing of a mouse skin wound infected by the multi-drug-resistant acinetobacter baumannii can be accelerated. In addition, the compositions of the present invention do not exhibit cytotoxicity at lower concentrations. Therefore, the composition disclosed by the invention can be used for preparing a medicine for resisting multi-drug-resistant acinetobacter baumannii infection.

The invention discloses a slow-release bacterial fertilizer as well as a preparation method and application thereof, and relates to the technical field of biotechnology. Comprising the following steps: step 1, preparing a bacterial liquid of bacillus cereus, a bacterial liquid of lysinibacillus slender, a bacterial liquid of acinetobacter schiffei, a bacterial liquid of stenotrophomonas maltophilia, a bacterial liquid of sphingobacterium alcohovorans and a bacterial liquid of bacillus licheniformis into a mixed bacterial suspension according to a volume ratio of 1: (1-2): (1-2): (1-2): (1-2): (1-2); 2, mixing the mixed bacterium suspension with an embedding agent and a cross-linking agent to obtain a first mixed colloid; step 3, embedding the first mixed colloid into the second mixed layer to obtain first compound fertilizer particles; and step 4, embedding the first compound fertilizer particles into a fermented third mixed layer to obtain the slow-release bacterial fertilizer. The prepared slow-release bacterial fertilizer improves the remediation effect of organic phosphorus contaminated soil. The soil fertility is improved, and a good growth promoting effect on plants is achieved.

The invention provides acinetobacter for culturing prawns in saline-alkali water and application of the acinetobacter. The preservation number of the provided acinetobacter is CGMCC (China General Microbiological Culture Collection Center) No.36066. The acinetobacter soilis strain provided by the invention has remarkable comprehensive application advantages, can still keep higher growth activity and metabolic capability in a low-temperature environment, and continuously plays a water body purification function. Ammonia nitrogen and nitrite nitrogen in a water body can be efficiently degraded, the environment of the aquatic water body is remarkably improved by promoting the nitrogen circulation process, and harm caused by nitrogen pollution is reduced. In addition, the strain can effectively inhibit and prevent reproduction of pathogenic vibrios and reduce the occurrence risk of bacterial diseases of aquatic animals. The strain is high in safety, does not cause poison or stress reaction to bred animals even under the condition of high-concentration use, and has good ecological safety and environmental friendliness.

The invention discloses acinetobacter baumannii and application thereof.The acinetobacter baumannii is preserved in Guangdong Microbial Culture Collection Center on August 16, 2024, the preservation number is GDMCC No: 65015, the acinetobacter baumannii has a good denitrification effect, and the degradation rate of ammonia nitrogen reaches 94.93%; the degradation rate on nitrate reaches 99.53%; and the degradation rate of nitrite reaches 96.94%. The immobilized microbial agent is further prepared from the bacillus subtilis and pseudomonas aeruginosa with vibrio parahaemolyticus resistance, and the immobilized microbial agent has good effects in the aspects of denitrification and vibrio parahaemolyticus resistance, especially shows better denitrification effect and antibacterial effect in actual culture application, and has good application prospects.

The present invention relates to the pharmaceutical field, and provides a boric acid β-lactamase inhibitor or a use thereof. The boric acid β-lactamase inhibitor is a compound shown in formula (I), or an optical isomer or a pharmaceutically acceptable salt thereof: formula (I). The ultra-broad-spectrum boric acid β-lactamase inhibitor of the present invention has an inhibition effect on A, B, C, and D enzymes, and carbapenem drug-resistant bacteria such as Klebsiella pneumoniae, Escherichia coli, Enterobacter cloacae, Acinetobacter baumannii, and Pseudomonas aeruginosa.

The present invention discloses an antimicrobial peptide and its application, wherein the antimicrobial peptide comprises one or more of an antimicrobial peptide PFK having an amino acid sequence as shown in SEQ ID NO: 1, an antimicrobial peptide PVK having an amino acid sequence as shown in SEQ ID NO: 2, and an antimicrobial peptide PIK having an amino acid sequence as shown in SEQ ID NO: 3. The antimicrobial peptides PFK, PVK and PIK of the present invention have antimicrobial effects on Porphyromonas gingivalis, Streptococcus mutans, Acinetobacter baumannii, Escherichia coli and Staphylococcus aureus, and the antimicrobial peptides PFK, PVK and PIK have low hemolytic activity and low cytotoxicity, and can be used to prepare biological antimicrobial preparations, anti-infective drugs or oral products.