497 results about "Acinetobacter" patented technology

The invention discloses a kit for quickly detecting 15 pneumonia pathogenic bacteria. The kit can detect streptococcus pneumoniae, staphylococcus aureus, haemophilus influenzae, mycoplasma pneumoniae, pseudomonas aeruginosa, baumanii, enterococcus faecalis, enterococcus faecium, klebsiella pneumoniae, escherichia coli, enterobacter cloacae, stenotrophomonas maltophilia, burkholderia cepacia, legionella pneumophila and chlamydia pneumoniae which cover clinically common pneumonia pathogenic bacteria difficult to culture. 16S rDNA and specific gene sequences corresponding to the pneumonia pathogenic bacteria are detected by combining gene chips with multiple asymmetric PCR reactions, and the categories of the bacteria in a to-be-detected sample are identified in genus and species. The kit makes up for the defect that current clinical detection of pneumonia pathogenic bacteria is not in time or comprehensive and a novel detection means for early diagnosis and early treatment of patients suffering from pneumonia is provided.

The invention discloses a composite microbial agent and a preparation method and application thereof. The composite microbial agent includes a pseudomonas preparation, an acinetobacter preparation, a bacillus amyloliquefaciens preparation, a bacillus subtillis preparation, a bacillus licheniformis preparation, a pediococcus acidilactici preparation, a rhodobacter sphaeroides preparation, an alkali-lake dietzia preparation and a rhodopseudomonas palustris preparation. The preparation method includes the following steps that 1, composite microbial agent components are prepared; 2, the composite microbial agent components are mixed in proportion to obtain the composite microbial agent. The composite microbial agent can simultaneously decompose different organic matter, degrade amino nitrogen and nitro nitrogen in water, reduce phosphorus concentration in the water body and inhibit the growth of algae. The composite microbial agent is used for urban-rural sewage in-situ remediation, has the advantages of being high in efficiency, quick to take effect, free of no secondary pollution, simple in operation and the like, and large-scale application and popularization are facilitated.

The invention discloses a microbial inoculum for bioremediating a coastline polluted by oil spilling and a preparation method thereof. The microbial inoculum for bioremediating the coastline polluted by oil spilling is made of mixed fermentation liquor and a fixed carrier as raw materials, wherein the mixed fermentation liquor comprises bacillus pumilus CGMCCnO.3384 fermentation liquor, Venice acinetobacter CGMCCnO.3385 fermentation liquor and/or Doxa pseudomonad CGMCCnO.3386 fermentation liquor and/or Ralstonia pickettii CGMCCnO.3387 fermentation liquor. By using the synergic action of different strains on the degradation of various petroleum hydrocarbons, the microbial inoculum improves the degradation speed of petroleum hydrocarbon pollutants and thoroughly degrades various petroleum hydrocarbon pollutants; in addition, a flora formed by a plurality of strains of bacteria has more stable structure and can better adapt to various petroleum-polluted environments. The microbial inoculum rapidly grows on the polluted areas, forms competitive advantages and remarkably increases the quantity of hydrocarbon degraded microbes in the polluted areas, thereby improving the biodegradation rate of crude oil.

The invention belongs to the production field of microbial fermentation, and in particular relates to an efficient grease degrading bacterium and an application thereof. The efficient grease degrading bacterium is of acinetobacter UC13, wherein the acinetobacter UC13 is preserved in CGMCC (China General Microbiological Culture Collection Center) on June 24th, 2013; the preservation number of the acinetobacter UC13 is CGMCC No:7818. The invention further relates to applications of the efficient grease degrading bacterium in treatment of kitchen wastes or petroleum hydrocarbon contaminants containing greases and in industrial refining relevant to the greases. A biological surfactant is a fermentation product obtained by fermenting the acinetobacter UC13. The biological surfactant is used for eliminating industrial greases and contaminants in the kitchen wastes containing the greases. The invention provides an extraction method of the biological surfactant. The extraction method is simple to operate and can be used for ensuring the high activity of the biological surfactant.

The invention relates to denitrification acinetobacters and a use thereof, and belongs to the field of environmental microbiology. The denitrification acinetobacters have a category name of Acinetobacter schindleri LN5, are preserved in the China general microbiological culture collection center of the China microbiological preservation management committee on May 30th, 2012, and has a preservation number of CGMCC No.6165. The denitrification acinetobacters have heterotrophic nitrification-aerobic denitrification functions, have high aureomycin resistance, can be used for simultaneous nitrification and denitrification (SND) treatment on nitrogen-containing sewage and especially on nitrogen and aureomycin-containing sewage, realize removal of a large part of ammonia nitrogen in nitrogen-containing sewage, realize small accumulation amounts of nitrite and nitrate nitrogen, realize nitrification-denitrification and COD removal in the same reactor, and realize environmentally-friendly, low-consumption and high-efficiency treatment on nitrogen-containing sewage.

The invention relates to a heterotrophic nitrification-aerobic denitrification compound bacterial agent for low-temperature high ammonia-nitrogen removal and application. The compound bacterial agentis formed by compounding of 10-20% of cupriavidus SWA1, 5-20% of alcaligenes faecalis, 10-30% of acinetobacter and 20-50% of ochrobacterum TAC-2. The compound bacterial agent has advantages of high ammonia-nitrogen resistance, low-temperature resistance, heavy metal resistance and synchronous nitrification and denitrification; by synergistic effects of four microorganisms, efficient denitrification of high ammonia-nitrogen wastewater can be realized in an aerobic environment under low-temperature conditions.

The invention discloses a complex endophytic bacterial inoculant and an application thereof in phytoremediation of heavy metal contaminated soil. The complex inoculant is composed of acinetobactercalcoaceticus, pseudomonas fluorescens, bacillusmegaterium, stenotrophomonasmaltophilia, bacillus pumilus and pseudomonas putida. The complex inoculant disclosed by the invention, when applied to heavy metal pollution remediation, integrates advantages of various endophytic bacteria and overcomes disadvantages of phytoremediation; and the complex inoculant can promote the growth of sedum alfredii hance, enhance the absorbing capacity of the sedum alfredii hance to heavy metals and improve the efficiency of phytoremediation.

The invention discloses acinetobacter baumannii, and a screening method and an application thereof in degradation of an azo dye Congo red. The stain is named as acinetobacter baumannii YNWH226, is preserved in China general microbiological culture collection center, and has the preservation number of CGMCC No.7922. The invention provides the new acinetobacter baumannii and the application thereof in degradation of the dye. Especially, the acinetobacter baumannii can effectively degrade the azo dye Congo red, has quite good decoloration and mineralization effects on Congo red wastewater, can completely mineralize the Congo red under a simple aerobic condition, and has great significance on governance of dye pollution and study on aerobic degradation of the azo dye.

The invention relates to a production method for synthesizing C8:0/C10:0/C12:0/C14:0 medium-chain fatty acid and ethyl ester thereof by using engineering Escherichia coli. The production method comprises the following steps of: cloning thioesterase genes with different specificities chFatB/ucFatB/ccFatB in cuphea/laurel/camphorwood into an expression vector pETDuet-1 to obtain a corresponding recombinant plasmid; transferring the recombinant plasmid into Escherichia coli BL21, performing isopropyl-beta-d-thiogalactoside (IPDT) induction expression, and specifically hydrolyzing acyl carrier protein (ACP) with specific chain length by using the expressed thioesterase to obtain medium-chain fatty acid; cloning acyltransferase gene (atfA) in acinetobacter into expression vectors pETDuet-chFatB/pETDuet-ucFatB/pETDuet-ccFatB to obtain corresponding recombinant plasmids; and transferring the recombinant plasmids into the Escherichia coli BL21, performing IPTG induction expression, performing acyl transfer reaction on exogenously fed ethanol and acyl-CoA formed by the medium-chain fatty acid intracellularly by using the co-expressed acyltransferase to obtain fatty acid ethyl ester.

The invention discloses a microorganism bacterium agent and a preparation method thereof, and relates to a composite bacterium agent and a preparation method thereof. The composite bacterium agent can be used for efficiently removing a large amount of COD (chemical oxygen demand), ammonia and nitrogen in leather wastewater. The composite bacterium agent is prepared from mycobacterium, microbacterium, acinetobacter, pichia pastoris, lactobacillus, klebsiella oxytoca and a liquid culture medium. The preparation method comprises the following steps of 1, activating the bacteria strains of raw materials; 2, enriching and culturing; 3, mixing the bacterium strains according to a weight ratio, and adding into the liquid culture medium, so as to obtain the composite bacterium agent. The composite bacterium agent has the advantages that the biological activity is high, the growth speed is high, the leather wastewater can be treated under the low temperature condition, the removal rate of organic matter can be obviously increased, the contents of COD, ammonia and nitrogen in the leather wastewater are effectively decreased, and the effect is obvious.

The present invention relates to a plasmid pUPI126 encoding indole-3 acetic acid (IAA) production; it also relates to a Acinetobacter strains having plasmid pUPI126; a bioinoculum for promoting growth of wheat plant, and lastly, it relates to a method of promoting wheat plant growth, said method comprising treating wheat seeds with the bioinoculum.

The invention discloses a conditioner for remediating organic contaminated soil by utilizing microbes. The conditioner comprises the following components in percentage by mass: 5-20% of microbial flora fermentation liquor, 20-50% of an organic improver and 30-75% of a culture medium, wherein the microbial flora relates to 19 bacterial genera comprising acinetobacter, micrococcaceae, bacillus, candida, trichoderma and aspergillus; the organic improver is one or several of diatomite, humus, straws, charcoal, saw dust, bagasse and plant ash; the culture medium contains monopotassium phosphate, dipotassium phosphate, ammonium sulfate, limestone and bean pulp. The conditioner disclosed by the invention contributes to degrading growth and reproduction of the microbial flora of organic pollutants, and can achieve effects of regulating the pH value of the soil and nutrition-allocated proportion of carbon, nitrogen and phosphorus, effectively improving the composition and structure of the organic contaminated soil and avoiding the phenomenon that the ventilation volume of soil is reduced due to hardening, so that the organic contaminated soil is effectively remediated.

The invention discloses bacteria capable of synchronously removing organic substances and ammonia nitrogen in water of a slightly polluted water source under low-temperature and aerobic conditions and a screening and domesticating method, which relate to bacteria and a screening and domesticating method and solve the problem that the prior bacteria are unsuitable for treating the slightly polluted water source as well as low temperature treatment. The bacteria SRA10 of the invention are acinetobacter lwoffi which belong to acinetobacter baumannii and are preserved in China General Microbiological Culture Collection Center on January 19th, 2009, with a preservation number of CGMCC No.2889. The screening method comprises the following steps: I, separating and purifying; II, preparing bacterial solution; III, screening and cultivating a bacterial solution; IV, screening and cultivating a bacterial solution with a live strain; V, repeating the step IV for 1 to 2 times, and stepwise domesticating the live strain; and VI, taking a bacterial solution of the domesticated strain, and rescreening the bacterial solution to obtain the bacteria. The bacteria obtained by screening and domesticating can process water of the slightly polluted water source under a condition of a low temperature of 2 to 10 DEG C.

The invention discloses an efficient denitrification novel Acinetobacter and application thereof. Acinetobacter guangzhouensis YZS-X1-1 is collected in China Center for Type Culture Collection on 6th August, 2014 under CCTCC NO. M2014369. The Acinetobacter has good denitrification capacity and is effective in removing ammonia nitrogen and nitrite nitrogen to protect the environment. The Acinetobacter can also be made into biological denitrification bacterial agents.

The invention discloses a method for improving the output of crude oil by utilizing a synergistic effect of combined bacteria liquid containing phosphate-solubilizing microorganisms and nitrogen-fixing bacteria. The method comprises the steps: injecting diluent of a compound microorganism bacterium agent prepared from pseudomonas fermentation liquor with a phosphate-solubilizing function, azotobacter fermentation liquor and combined bacteria liquid with a petroleum hydrocarbon degrading function into an oil pool via a water injection well or injecting through a production well, and extracting oil to obtain the crude oil. The compound microorganism bacterium agent is prepared from the pseudomonas fermentation liquor, the azotobacter fermentation liquor and the combined bacteria liquid with the petroleum hydrocarbon degrading function according to a ratio of 1 to 1.2 to 1.6-8; the combined bacteria liquid with the petroleum hydrocarbon degrading function contains bacillus, acinetobacter and xanthomonas; and the three bacteria are independently cultivated into single bacteria liquid of 108-1010cfu/ml, and then mixed iso-volumetrically, so that the combined bacteria liquid with the petroleum hydrocarbon degrading function is obtained. According to the invention, the method is simple in construction, short in time, low in cost, large in yield rate and high in oil extraction productivity.

The invention discloses a heavy-metal-resisting microbial agent, a preparation method and an application thereof. The preparation method of the microbial agent comprises the following steps of: (1) respectively carrying out liquid culture in a fluid medium after activating acinetobacter calcoacetcus with the preservation number of CGMCC No.6820 and pseudomonas aeruginosa with the preservation number of CGMCC No.6821 to obtain bacterial liquid A and bacterial liquid B; and (2) inoculating the mixed bacterial liquid A and B to a solid fermentation medium, ventilating, stirring and fermenting for 24-48 hours at 28-38 DEG C, and drying in vacuum to obtain the heavy-metal-resisting microbial agent. The heavy-metal-resisting microbial agent disclosed by the invention can not only remove a part of heavy metal ions, but also can remove a major of organic pollutants.

The present invention provides a disinfectant composition including a phage of Acinetobacter baumannii and a carrier. The present invention also provides a method for disinfecting a medical institute or a medical research institute, including the steps of applying an effective amount of a phage of Acinetobacter baumannii to the medical institute or the medical research institute for reducing amount of Acinetobacter baumannii in the medical institute or the medical research institute.

The invention discloses an aerobic denitrifying strain, which is classified and named as Acinetobacter sp. Strain N2. The strain is preserved in China General Microbiological Culture Collection Center on March 19, 2013, and the preservation number is CCTCC NO: M2013093. The invention also discloses application of the aerobic denitrifying strain. The invention adopts the high efficiency aerobic denitrifying strain screened from a eutrophic lake water body. The strain has a heterotrophic nitrification effect, and can simultaneously remove nitric nitrogen and ammonia nitrogen from the water body. The denitrification process accumulate little nitrite and has no secondary pollution. After expansion culture, the strain can be directly applied to denitrification of lake water, and environment inadaptation of non-indigenous strains can be avoided. Thus, the strain has great application potential in practical engineering.

The invention relates to a repairing agent for microbes in water bodies of excessive culture zones, which comprises the following bacteria: Bacillus, Pseudomonas, Bacillus pumilus and Acinetobacter; the collection institution is the China General Microbiological Culture Collection Center (CGMCC); the collection numbers are respectively CGMCC No: 3737, 3739, 3752 and 3712; and the mass ratio of the Bacillus to the Pseudomonas to the Bacillus pumilus to the Acinetobacter is 1.4-1.7:1.0-1.4:0.9-1.1:1.2-1.5. The repairing agent of the invention is a microbial ecological agent which can be used for repairing excessive culture zones of water environment, is harmless to culture organisms, can inhibit propagation and growth of harmful microbes in water bodies, purifies the water quality and improves the quality and the performance of aquatic products.

Relating to the field of microorganisms, the invention discloses an inoculant, a feed or additive and a removal method for mycotoxins. The invention specifically discloses an inoculant, which includesat least two of mould, yeast and bacteria, wherein the bacteria can be at least one of brevibacterium, acinetobacter, rhodococcus and pseudomonas. Specifically, the inoculant can remove mycotoxins. The invention also discloses application of the inoculant in removal of mycotoxins, an additive containing the inoculant and a removal method for mycotoxins. According to the technical scheme, combineduse of at least two of the yeast, mould and bacteria can achieve simultaneous removal of ochratoxin A, fumonisins and T-2 toxin, and the removal efficiency of ochratoxin A and fumonisins is the highest.

The invention dislcoses acinetobacter baumannii capable of efficiently degrading imazamox, which relates to acinetobacter baumannii. The invention provides an acinetobacter baumannii strain, which is capable of efficiently degrading imazamox and provides theoretical and microbial resource foundations for the environmental modification and degradation mechanism research of an imazamox weedicide. The acinetobacter baumannii capable of efficiently degrading imazamox, disclosed by the invention, is acinetobacter baumannii IB5 which is collected in the China General Microbiological Culture Collection Center (Address: No.3, Yard 1, Beichen Road West, Chaoyang District, Beijing) with the collection number CGMCC No.4728 on April 1, 2011. The acinetobacter baumannii IB5 is capable of efficiently degrading imazamox.

The present invention discloses a strain of marine Bacillus amyloliquefaciens B01, wherein the strain is preserved in the China general microbiological culture collection center on December 11, 2014, and has the preservation number of CGMCC No.10154. According to the present invention, the fermentation broth supernatant of the Bacillus amyloliquefaciens B01 can inhibit aquatic diseases caused by a variety of pathogenic bacteria such as Acinetobacter baumannii, Vibrio parahaemolyticus and the like, and the marine Bacillus amyloliquefaciens B01 has characteristics of no toxicity on human and animals, green environmental protection, aquatic pollution disease prevention and control effect, and good biological pesticide development prospects.

The invention provides a novel algin lyase producing strain, namely Acinetobacter juni. X8 (which is preserved in the China Center for Type Culture Collection, wherein the preservation number is CCTCCC No: M 209110 and the preservation time is May 20, 2009) and application thereof in preparing algin lyase. The Acinetobacter juni. X8 has the following advantages: (1) the nutritional requirement is simple, the cultivation is easy, the generation time is short, and the fermentation cultivation time is short; (2) intracellular crude enzyme solution is simple to prepare, and a centrifugal collected thallus can be shattered directly after being added into buffer solution; (3) the algin lyase prepared from the Acinetobacter juni. X8 has higher enzyme activity, and unseparated and unpurified crude enzyme solution can reach 53.9 U/mg (157.3 U/mL); (4) the reaction conditions required by the enzyme enzymolysis algin is mild, and the acting time is short; and (5) the algin lyase has good stability, can keep higher enzyme activity (more than 97 percent) after being preserved for 5 days at a temperature of less than or equal to 4 DEG C, and is stable for 8 hours under a condition of which the pH is between 7.7 and 9.0.