60 results about "Bacteroides" patented technology

The invention discloses an intestinal bacterial composition for improving a cognitive function and relieving neuroinflammation and an application of the intestinal bacterial composition, and belongs to the technical field of biomedicine and microbiology, the intestinal bacterial composition is composed of a Lachnospiraceae bacterium, a Bacteroides multiforme and a Roseburiatestinae bacterium, and the intestinal bacterial composition is prepared from the following raw materials of the following raw materials of the intestinal bacterial composition: the Lachnospiraceae bacterium, the Bacteroides multiforme and the Roseburiatestinae bacterium, the Roseburiatestinae bacterium, the Roseburiatestinae bacterium, the Roseburiatestinae bacterium, the Roseburiatestinae bacterium, the Roseburiatestinae bacterium and the like. Experimental research results show that a modular synergistic effect exists among the helicobacter hirsuticus, the bacteroides multiforme and the Roche enterobacter, and the pharmaceutical composition has a remarkable neuroprotective effect on a cognitive impairment mouse model and an Alzheimer disease mouse model, can improve the cognitive function and relieve neuroinflammation, and can be used for preparing a neuroprotective agent for treating the cognitive impairment mouse model and the Alzheimer disease mouse model. A new thought and method can be provided for treatment of cognitive impairment related diseases and neuroinflammation related diseases.

The invention discloses a bifidobacterium animalis subsp. Lactis BA-C53 and an application of the bifidobacterium animalis subsp. Lactis BA-C53 in products for improving bone health. The bifidobacterium animalis subsp. Lactis BA-C53 is classified and named as bifidobacterium animalis subsp. Lactis Bifidobacterium animalis subsp. Lactis BA-C53, the preservation number is CCTCC NO: M 2024460, the preservation unit is China Center for Type Culture Collection, and the preservation time is March 11, 2024. The bifidobacterium animalis subsp. Lactis BA-C53 can effectively improve the steady state of intestinal flora of a mouse with osteoporosis induced by estrogen deficiency, so that the relative abundance of phylum firmicalis and phylum actinomycetes is increased, the relative abundance of phylum bacteroides is reduced, and then short-chain fatty acid is generated to activate intestinal immunity and maintain the integrity of an intestinal barrier, so that the osteoporosis caused by estrogen deficiency is inhibited. Meanwhile, osteoclast differentiation can be inhibited by regulating NFATC1, RANKL and OPG pathways, then osteoporosis is relieved, and the compound can be widely applied to preparation of food and drugs.

The invention relates to a biomarker for screening intestinal flora transplantation donors and application of the biomarker. The biomarker comprises intestinal flora and metabolites thereof, the intestinal flora comprises phylum firmicalis, bacteroides and the like, and the metabolites comprise short-chain fatty acid, tryptophan, indole, 5-hydroxytryptamine, lipopolysaccharide and the like. According to the method, the intestinal flora of different crowds is deeply analyzed, the relationship between each related index of the intestinal flora and the intestinal flora transplantation effect is studied, sufficient clinical verification is carried out, a biomarker for screening the intestinal flora transplantation donor is excavated, and a standard for judging whether the intestinal flora transplantation donor is qualified or not is established; and a new method and a new idea are provided for efficiently and accurately screening intestinal flora transplantation donors.

The embodiment of the invention discloses application of lactobacillus delbrueckii. The application is characterized in that the lactobacillus delbrueckii is applied to inhibition of mesophilic aerobic bacteria, saccharomycetes, mould and coliform bacteria. The lactobacillus delbrueckii has a preservation number of CGMCC No.11247. In the lactobacillus delbrueckii activating process, bacteriocin can be generated and can remarkably inhibit the mesophilic aerobic bacteria, saccharomycetes, mould and coliform bacteria on the surfaces of fruits and vegetables, so that the preservation effect of thefruits and vegetables can be improved. Furthermore, secondary metabolites of the lactobacillus delbrueckii can be biodegraded and digested by human bodies, and are healthy and harmless to human bodies.

This invention belongs to the field of intestinal microbiome technology, specifically relating to a capsular polysaccharide derived from Bacteroides salorlis CSP6, its preparation method, and its applications. The monosaccharide composition of the capsular polysaccharide is mainly composed of fucose, glucose, and glucosamine, with a molecular weight of 7.139 × 10⁻⁶. 5 The strain described is deposited at the China Center for Type Culture Collection (CCTCC), accession number CCTCC NO: M20221993. In vitro studies have found that the capsular polysaccharide derived from Bacteroides Salieris CSP6 can inhibit α-glucosidase; in vivo animal experiments have demonstrated that this capsular polysaccharide can inhibit the rise in postprandial blood glucose and has a hypoglycemic effect.

The invention provides a preparation method of an apple water kefir beverage and application of the apple water kefir beverage in lowering cholesterol. The method comprises the following steps: preparing apple juice, activating water kefir grains, inoculating the activated water kefir grains into the apple juice, and fermenting to prepare the apple water kefir beverage. The volatile component change in the fermentation process is analyzed through GC-MS and GC-IMS systems, it is proved that the unique bouquet characteristic and flavor coordination of the apple water kefir beverage are improved through water kefir grain fermentation, and meanwhile the organic acid composition is optimized. A mouse model verifies that the apple water kefir beverage has the effect of reducing cholesterol, can reduce liver lipid droplets, total cholesterol, low-density lipoprotein and triglyceride levels, and also can regulate intestinal microbial communities (increase firmicobacteria, bacteroides and faecalis bacteria). The apple water kefir beverage disclosed by the invention has wide market potential and provides a scientific basis for the development of functional beverages.

The invention discloses application of acid-producing bacteroides in treatment of hepatic fibrosis and hepatocellular carcinoma. In-vitro cell experiments verify that the Bacteroides acidogenes and the culture medium thereof can inhibit LX2 cell fibrosis induced by TGF-beta, can inhibit the activity of LX2, Huh7 and 97H cells and can inhibit the cell proliferation capacity at the same time, and further, in a hepatocellular carcinoma spontaneous animal model induced by diethylnitrosamine and carbon tetrachloride, the application of the Bacteroides acidogenes and the culture medium of the Bacteroides acidogenes and the culture medium of the Bacteroides acidogenes and the culture medium of the Bacteroides acidogenes in the hepatocellular carcinoma spontaneous animal model has the advantages of being capable of inhibiting the fibrosis of the LX2 cells induced by the TGF-beta and inhibiting the proliferation capacity of the cells. The inhibition effect of the acid-producing bacteroides on hepatic fibrosis and liver cancer progression is verified; the acid-producing bacteroides is recognized as probiotics and has low side effect risk, so that the acid-producing bacteroides has a good clinical application prospect.

The invention belongs to the technical field of organic substrates, and particularly relates to an organic substrate preparation process based on complex microbial inoculant synergistic fermentation, which comprises the following steps: selecting agricultural wastes, livestock and poultry manure and food industry byproducts, and carrying out staged fermentation by adopting complex microorganisms such as bacillus, streptomyces, clostridium and bacteroides; the decomposition efficiency of degradation-resistant substances such as cellulose and lignin is obviously improved. By combining precise temperature control and oxygen concentration adjustment strategies, the stability of the fermentation process is enhanced, nitrogen volatilization is reduced, and the total nitrogen content is stabilized at 1.2-1.5%. Nitrogen-fixing bacteria and phosphate solubilizing bacteria are introduced in the after-ripening stage, the biological activity of the matrix is improved, the screening process ensures that the finished product is uniform in particle size and few in impurities, the content of organic matter reaches 45% or above, the pH value is stabilized at 6.5-7.5, excellent agricultural performance is achieved, and efficient resource utilization of organic waste is achieved; the problems that in the prior art, organic matter degradation is not thorough, the controllability of the fermentation process is poor, nutrient loss is serious, and the quality of a finished product is unstable are effectively solved.

The main purpose of the present invention is to provide a Monascus pilosus and a composition for reducing weight and improving intestinal flora, the composition comprising the Monascus pilosus and / or a monascus fermentation product thereof, or a functional component comprising the monascus fermentation product. The invention also aims to provide application of the monascus in preparation of the composition for reducing weight and improving intestinal flora. The composition disclosed by the invention has the capabilities of reducing body weight and improving intestinal flora, such as reducing the ratio of the phylum firmicutes to the phylum Bacteroides.

The invention discloses a Lactobacillus paracasei-composite prebiotic compound preparation, a preparation method and application thereof, and belongs to the technical field of microbial preparations. The compound preparation is prepared from the lactobacillus paracasei PC-92 and compound prebiotics in an optimized compatibility mode, the compound prebiotics are composed of trehalose, inulin and fructo-oligosaccharide, and the adding amount of the compound prebiotics is as follows: 1.959% of the trehalose, 1.029% of the inulin and 1.582% of the fructo-oligosaccharide. According to the casei paracasei-composite prebiotic compound preparation, the preparation method and the application thereof, the compound preparation can remarkably improve intestinal flora disorder of mice with the ulcerative colitis induced by DSS, adjust abundance of key flora such as lactobacillus and bacteroides, reduce disease activity indexes, maintain intestinal microecological balance and improve the intestinal immunity of the mice. The method can be applied to the field of functional food, health care products or medicines, and provides a new scheme for intestinal health intervention.

The application discloses a method for identifying direct discharge of domestic sewage and application thereof. The method comprises the following steps: detecting the copy number concentration of bacteriophage marker genes and Bacteroides marker genes of adjacent two days of a water body to be detected respectively, calculating the ratio of the copy number concentration of the bacteriophage marker genes and the copy number concentration of the Bacteroides marker genes per day, and calculating the ratio between the two days of the water body to be detected, and combining a specific ratio threshold to determine whether the direct discharge of domestic sewage appears in the water body to be detected, and the day when the direct discharge of domestic sewage appears can be accurately determined, so that the direct discharge of domestic sewage can be traced and controlled; wherein the bacteriophage marker gene is CPQ_064, and the Bacteroides marker gene is BacHum, BacH, HuBac and / or HF183.

The invention discloses a kit for detecting intestinal core bacteria by a TaqMan real-time fluorescent quantitative PCR (Polymerase Chain Reaction) method, and belongs to the technical field of kits. The kit disclosed by the invention comprises standard positive templates of nine common intestinal core bacteria and nine groups of specific primers and probes which are required in a PCR amplification process and correspond to the standard positive templates. The kit disclosed by the invention is simple, convenient and rapid to operate; the specificity is good and the sensitivity is high; complicated raw information data analysis does not need to be carried out; the intestinal core bacteria can be detected and quantified without high-throughput sequencing, and three bacteria can be detected in one reaction hole, so that the cost is greatly reduced. The kit provided by the invention can be used for rapidly and quantitatively detecting nine intestinal core bacteria including bifidobacterium, lactobacillus, prevotella, bacteroides and the like, can partially replace a high-throughput detection method which is higher in cost and more complicated to operate according to research and diagnosis purposes, and is suitable for being widely popularized in clinical detection and laboratory research.

Engineered Bacteroides thetaiotaomicron (Bt) strains, including deletion mutant strains, and methods of use thereof are provided. Methods of delivering a therapeutic compound to a gut microbiome of a subject include generating an engineered Bacteroides thetaiotaomicron (Bt) strain to overproduce outer membrane vesicles (OMVs); loading a target therapeutic compound into the OMVs; and colonizing the gut microbiome of the subject with the engineered Bt strain. In some embodiments, the subject has at least one of lactose intolerance, phenylketonuria, inflammatory bowel disease, and a chronic intestinal condition.

This invention, entitled "A Composition Containing Pentadecanoic Acid, Parabacterium Species Outer Membrane Vesicles Containing the Composition, and Their Applications," belongs to the field of probiotics and microbial preparations technology. The technical problem this invention aims to solve is that beneficial live bacteria have weak colonization ability in the gut, are easily affected by the gastrointestinal environment, exhibit unstable individual responses, and struggle to simultaneously address immune regulation and barrier repair. This invention provides a composition containing pentadecanoic acid, Parabacterium Species-derived outer membrane vesicles, and their applications. The outer membrane vesicles are derived from *Parabacterium difficile* and are rich in pentadecanoic acid, palmitic acid, and octadecanoic acid. The composition and outer membrane vesicles prepared by this invention can significantly inhibit macrophage M1 polarization and reduce pro-inflammatory cytokines. TNF-α , IL-6 and IL-1 β It can lower the expression level of [something], reduce intracellular reactive oxygen species levels, improve mitochondrial function, significantly alleviate symptoms of colitis induced by sodium dextran sulfate, regulate the intestinal flora structure, and increase the abundance of beneficial bacteria.

This invention belongs to the field of intestinal microbiome technology, and particularly relates to the application of a capsular polysaccharide derived from Bacteroides Salilsii CSP6 in the treatment and / or prevention of inflammatory bowel disease. The Bacteroides Salilsii strain is... Bacteroides salyersiae CSP6. This strain was isolated from the intestine of healthy humans and deposited at the China Center for Type Culture Collection (CCTCC), accession number CCTCC NO: M 20221993. In vivo experiments showed that the capsular polysaccharide derived from Bacteroides Salieris CSP6 can protect the colorectal mucosa from damage and promote the repair of damaged intestinal mucosa, thereby improving symptoms such as loose stools, rectal bleeding, and shortened colon and rectum, reducing weight loss caused by intestinal damage and inflammation, and ultimately exerting a therapeutic effect on inflammatory bowel disease.

The invention discloses an application of premna microphylla pectin in preparation of a product for preventing and improving diet-induced obesity. The premna pubescens pectin is prepared from premna pubescens leaves as a raw material through extraction, purification and other processes. According to the present invention, the research on the high fat diet induced obese mouse model finds that the body lipid metabolism is regulated by remodeling the intestinal flora structure, particularly reducing the ratio of the phylum firmicutes to the phylum bacteroides, and specifically enriching the beneficial flora such as Muribacaceae and the like by using the premna microphylla pectin; experimental results prove that the premna microphylla pectin can significantly inhibit pathological hypertrophy of adipocytes, reduce visceral fat accumulation, effectively control weight gain and improve dyslipidemia and liver fatty degeneration, and has good biological safety.

To find out a useful living body-regulating function in a treated product of Abelmoschus manihot and to provide a functional use thereof.SOLUTION: An intestinal environment-improving agent comprising a processed product of Abutilon striata as an active ingredient, wherein the intestinal environment-improving agent increases bacteria of the genus Lactobacillusmurinus and / or the genus Parabacteroides.SELECTED DRAWING: Figure 5

The invention belongs to the technical field of biology, and discloses application of chitosan oligosaccharide in preparation of a culture medium for in-vitro culture of fecal flora, a fermentation culture medium and an in-vitro culture method of the fecal flora. The chitosan oligosaccharide is introduced into in-vitro culture of fecal flora, so that cross feeding among the flora can be well amplified to form an efficient synergistic metabolism network, the capability of taking and utilizing quick-acting nutrients such as glucose and peptone of probiotics such as prevotella, bacteroides and streptococcus is improved, the nutrient competitive advantage of the probiotics is improved, and the effect of improving the fecal flora is achieved. The improvement of the nutritional competitive advantage can further strengthen the synergistic metabolism network of the probiotics to form positive feedback circulation, so that the probiotics are stably proliferated and occupy the advantages, and the obtained flora retains the richness and diversity similar to those of the original excrement flora, and has the advantages of being simple in structure and convenient to use. Wherein the contained prevotella, bacteroides and streptococcus are remarkably enriched, and the strain has huge application potential in the in-vitro precise culture of fecal flora.

The application discloses Bacteroides multipara and application thereof for relieving metabolic dysfunction related fatty liver disease, and belongs to the technical field of microorganisms. Phocaeicola dorei The Bacteroides multipara N02 can inhibit the increase of liver and the increase of liver weight / body weight of a mouse with metabolic dysfunction related fatty liver disease without affecting the food intake, can regulate the lipid metabolism disorder of the mouse with metabolic dysfunction related fatty liver disease and relieve the liver tissue damage, and can improve the intestinal barrier function of the mouse with metabolic dysfunction related fatty liver disease. The Bacteroides multipara N02 has a very wide application prospect when used for preparing a medicine composition for relieving metabolic dysfunction related fatty liver disease and a fermented food.

Methods and compositions comprising recombinant fusion polypeptides that are engineered to allow for heterologous cargo secretion from a Bacteroides cell, into an extracellular environment are provided. Provided methods and compositions include recombinant fusion polypeptides comprising a secretion domain and a heterologous cargo domain. Methods and compositions for using said recombinant fusion polypeptides, e.g., for the treatment of a disease and / or disorder, are also provided.

The invention relates to application of a compound FK3 in preparation of a product for improving intestinal flora disorder, and belongs to the field of medicines. The invention provides a novel application of a compound FK3 or a pharmaceutically acceptable salt thereof in preparation of a product for improving intestinal flora disorder or treating and / or preventing intestinal flora disorder related diseases, and the structural formula of the compound FK3 is shown in the specification. The compound FK3 improves intestinal flora disorder by remarkably increasing the abundance of intestinal core probiotics such as helicobacter pylori and bacteroides and remarkably reducing the abundance of harmful bacteria such as helicobacter pylori and bacteroides and further improves anxiety and social behaviors of autism, has a very wide application prospect, and has a broad application prospect. And a solid foundation is provided for clinical application.

The invention discloses a co-fermentation method for promoting proliferation of lactobacillus rhamnosus based on pre-degradation of pectin by bacteroides multiforme, and belongs to the technical field of food processing. The co-fermentation method specifically comprises the following steps: by taking pectin with the molecular weight of 10kDa as a substrate, pre-inoculating bacteroides multiforme for fermentation so as to degrade the pectin into oligosaccharide fragments, and then inoculating lactobacillus rhamnosus for co-culture. The utilization capacity of the lactobacillus rhamnosus on the pectin is remarkably improved by oligosaccharide fragments generated by pre-degrading the pectin through the bacteroides polymorpha, so that the efficient proliferation of the lactobacillus rhamnosus is promoted. Compared with traditional single fermentation, the biomass of lactobacillus rhamnosus can be increased by 40% or above. The method simulates the natural process of synergistically metabolizing pectin by intestinal flora, is beneficial to development of fermentation preparations closer to intestinal microecology, can be widely applied to production of feed probiotic preparations, and provides a new technical support for improving the probiotic effect of pectin and developing intestinal microecology directional regulation and control products.

The invention discloses a preparation method of a ficus microcarpa extract and application of the ficus microcarpa extract in intestinal flora disorder caused by circadian rhythm disorder. Pharmacological experiments prove that the ficus striata flavone has a remarkable regulating effect in a circadian rhythm disorder injury model: the ficus striata flavone can be used for effectively solving the problems that the diversity of intestinal flora is reduced, the excessive propagation of firmictures, the expression of Bacteroidetes is reduced and the like, which are caused by circadian rhythm disorder, and can be used for preventing and treating the diseases caused by circadian rhythm disorder, so that the ficus striata flavone can be used for preventing and treating the diseases caused by circadian rhythm disorder. Meanwhile, circadian rhythm oscillation of key bacteria such as prevotella, Bacteroides and Clostridium can be adjusted, and increase of the F / B ratio is reduced, so that the rhythm and stability of intestinal flora are improved. The invention simultaneously discloses two preparation processes, namely an ethanol reflux-resin purification method and a water extraction-concentration-alcohol precipitation method, so that the stable quality of the extract is ensured, and the industrial feasibility is realized.

The invention discloses high-activity and thermal-stability PL29 family hyaluronic acid lyase derived from bacteroides and application of the PL29 family hyaluronic acid lyase, and belongs to the technical field of microorganisms. The invention provides the hyaluronic acid lyase BfiHAase derived from Bacteroides pinnatae, the hyaluronic acid lyase BfiHAase has good enzymatic activity and thermal stability, the optimal reaction pH value of the BfiHAase is 6.08, the optimal reaction temperature is 40 DEG C, the hyaluronic acid lyase BfiHAase has high enzyme activity, and the highest enzyme activity under the optimal condition can reach 454.9893 U / mg and exceeds that of most hyaluronic acid lyase. Meanwhile, the enzyme has good thermal stability, can keep more than 90% of activity after being stored at 4 DEG C for 6 hours, and can still keep more than 70% of activity after being stored for one day, which indicates that the enzyme has great industrial application potential.

Abstract:　Problem: To provide a soil-transmissible plant disease control agent or similar that can safely be used as a biological control agent.　Solution: A soil-transmissible plant disease control agent or similar, such as a vegetable bacterial wilt control agent, can be provided by using, as an active ingredient, live bacterial cells of a novel strain of a bacterium of the Bacillus or Fictibacillus genus with a heretofore unknown soil-transmissible plant disease controlling action, or a cultured product including said live bacterial cells. This control agent is characterized by being able, through a foliage application treatment, to control bacterial wilt and / or bacterial soft rot caused by the Ralstonia solanacearum species complex, which are soil-borne bacteria.　Selected drawing: none

The invention provides application of bacteroides faecalis as a target spot in detection and promotion of hot habit. Through systematic metagenomics analysis on excrement samples of hot-area crowds and warm-area control crowds and through difference comparison and verification research, the characteristic microbial marker bacteroides faecalis significantly related to the hot habit state is identified for the first time. The method can accurately reflect the adaptive state of an individual to the thermal environment, and can be used for non-invasive evaluation or auxiliary detection of the thermal habit degree. Based on the marker, the probability that an individual is in a hot habit state or a non-habit state can be quantitatively evaluated, and a new biomarker detection method is provided for objective diagnosis of hot habit.

The invention relates to a culture medium for screening bacteroides vulgaris and application of the culture medium, and belongs to the technical field of microorganisms. The enrichment culture medium and the isolation culture medium with specific components are designed, the enrichment culture medium can effectively promote growth of common bacteroides and rapidly enrich common bacteroides in a sample, the isolation culture medium has good selectivity, growth of most strains except common bacteroides is inhibited, efficient isolation and screening are achieved, the screening efficiency is remarkably improved, and the screening cost is reduced. The development of the common bacteroides field is promoted.

The invention belongs to the technical field of functional foods and medicines, and particularly discloses application of L-selenium methyl selenocysteine in preparation of a product for targeted regulation of intestinal flora to relieve chemotherapy immune injury. The target regulation is realized by specifically improving the relative abundance of the beneficial bacteria, namely, Bacteroides acdifaciens, in the intestinal tracts of the subject, so that the relative abundance of the beneficial bacteria, namely the Bacteroides acdifaciens, in the intestinal tracts of the subject is improved. Systematic contrast experiments prove that under the condition of equal selenium content, the selenium-enriched milk powder strengthened by taking L-selenium methyl selenocysteine as a unique selenium source has the effect obviously superior to that of contrast milk powder strengthened by selenium-enriched yeast or sodium selenite in the aspect of relieving immunosuppression, intestinal injury and systemic inflammation caused by cyclophosphamide. The core mechanism of the L-selenium methyl selenocysteine is that the L-selenium methyl selenocysteine can be used for efficiently and specifically enriching Bacteroides acdifaciens, and the L-selenium methyl selenocysteine can be used for efficiently and specifically enriching Bacteroides acdifaciens. Based on the discovery, the invention correspondingly provides a selenium-enriched milk powder product with definite targeting property and excellent effects, and a brand new efficient solution is provided for chemotherapy auxiliary nutrition support of tumor patients.

The present invention provides a technology that can effectively inhibit the proliferation of Bacteroides stercolis and a technology that can enhance sensitivity to immune checkpoint inhibitors. According to the present invention, the proliferation of Bacteroides stercolis can be inhibited in vivo. Furthermore, according to the present invention, sensitivity to immune checkpoint inhibitors can be enhanced. This can contribute to improving the response rate or the therapeutic effect of the target disease, and ultimately to improving the quality of life of patients. SOLUTION: A growth inhibitor of Bacteroides stercoli, which contains agarooligosaccharide as an active ingredient.

The invention relates to the technical field of environmental microorganisms, and particularly discloses a sulfate reducing bacteria mixed bacteria solution for mine acid wastewater treatment as well as a preparation method and application of the sulfate reducing bacteria mixed bacteria solution. The sulfate reducing bacteria mixed bacteria solution disclosed by the invention is prepared from the following strains with relative abundance: 28.0 to 35.0 percent of bacteroides, 13.0 to 16.0 percent of toathouse bacillus, 8.0 to 12.0 percent of desulfation coccus, 2.5 to 3.6 percent of desulfation leaf bacteria, 2.0 to 2.7 percent of desulfation vibrio and 0.9 to 1.7 percent of sulfuration spirillum. The method comprises the following steps: taking a mine acid wastewater sediment sample as a raw material to prepare a sulfate reducing bacteria mixed bacteria solution; the bacterial liquid can reduce sulfate in the mine acid wastewater into sulfide, generate alkalinity, increase the pH value of the mine acid wastewater, and remove heavy metals in the mine acid wastewater in a precipitation form, so that the mine acid wastewater is harmless, and effective treatment of the mine acid wastewater is realized.