307results about How to "Improve tolerance" patented technology

The invention discloses engineered saccharomyces cerevisiae and application of the engineered saccharomyces cerevisiae in synthesis of 2-phenethyl alcohol, and belongs to the technical field of genetic engineering. According to the engineering saccharomyces cerevisiae, saccharomyces cerevisiae CICC 33068 is modified, and the strain is modified through strategies such as aldrin pathway enhancement, precursor enhancement, tolerance optimization and cofactor balance. The constructed engineered strain has good metabolic performance and stability, not only is the tolerance of the engineered strain to 2-phenethyl alcohol improved, but also the production capacity of the 2-phenethyl alcohol is remarkably improved. Therefore, the engineered saccharomyces cerevisiae disclosed by the invention has remarkable advantages in production of the 2-phenethyl alcohol, can provide technical guidance for industrial production of the 2-phenethyl alcohol, and has a good application prospect.

The invention provides haematococcus alternatus and application thereof. The classification of the haematococcus alternatus is named as Alterrythrobacter sp. H2, and the classification of the haematococcus alternatus is named as Alterrythrobacter sp. The preservation unit is China General Microbiological Culture Collection Center (CGMCC); the address is Institute of Microbiology, Chinese Academy of Sciences, No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing; the preservation date is July 29, 2024, and the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.31487. The alternated red bacillus provided by the invention can degrade various polycyclic aromatic hydrocarbons under various environmental conditions of high salt, high temperature, low temperature, acidity, alkalinity, chromium combined pollution, arsenic combined pollution and the like.

The invention discloses a wide-working-condition steam turbine system operation data correction method based on digital twinning, and relates to the technical field of steam turbine equipment digitalization, and the method comprises the steps: collecting operation data of a wide-working-condition steam turbine system in real time; constructing a digital twinborn model of the steam turbine system; performing prior covariance estimation on the operation data according to the statistical distribution condition and the design parameters; generating a Sigma point by adopting an unscented Kalman filtering algorithm, iteratively updating a state vector and a covariance in combination with a state transition equation and an observation equation, and outputting an updated known variable; performing smoothing processing on the updated known variable in a plurality of time point regions by adopting an RLOESS algorithm to obtain corrected operation data; according to the method, the real dynamic characteristics of the wide-working-condition steam turbine are approached by building the digital twinborn model, the calculation complexity of dynamic data fusion of a steam turbine system can be remarkably reduced, and the problems that the convergence speed is low and calculation is complex due to strong nonlinearity of a traditional method are solved.

The present application provides a diaphragm-free lithium ion battery. The battery comprises a positive electrode current collector, a positive electrode active material, a solid electrolyte, a solvent, a negative electrode active material, and a negative electrode current collector. The solid electrolyte is a solid porous organic polymer, which contains specific functional groups, including weakly coordinated anion groups, and the corresponding cation of the anion group is lithium ion. The porous polymer is a solid powder, which can act as a barrier between the positive electrode active material and the negative electrode active material, eliminating the need for a diaphragm and greatly improving the safety performance of the battery.

The application discloses a kind of hybrid power system health management method and system, belong to power system health management field.This application selects the characteristic signal of engine, battery and vehicle in hybrid power system to establish hybrid power system health degree;When hybrid power system fails, by obtaining characteristic signal and carrying out normalization processing, hybrid power system health degree based on grey correlation analysis method GRA is calculated in real time, and the misjudgment and the missed judgment of fault are reduced by the fault confirmation method based on counting;When hybrid power system fault is confirmed, through the power redistribution health management strategy under system fault based on LSTM-MPC, the change of hybrid power system speed, battery temperature and diesel engine exhaust temperature in limited time domain is predicted, and the predictive maintenance of hybrid power system health degree is carried out in combination with the change prediction result of part of the state of hybrid power system, so that hybrid power system can still operate with fault under the condition of meeting constraint.

The present application relates to the technical field of radar signal processing, aiming at the target positioning and imaging ambiguity caused by the distance-angle coupling of the traditional frequency diversity array beam pattern, and the problem that more array elements are needed for random frequency offset to remove ambiguity, a FDA structure based on joint design of intra-array frequency offset and inter-array frequency offset is proposed, the transmission energy is focused on the expected distance-angle area to realize distance-angle decoupling, and by increasing the number of intra-array frequency increments, the number of array elements is increased to improve the target ambiguity-free limit, the number of frequency increments is increased from N of the traditional FDA to, the order of magnitude of the ambiguity-free limit can be greatly increased, and the tolerance of the target ambiguity-free limit is greater. By optimizing the parameters of the array element and inter-array frequency offset design, the de-ambiguous imaging can be realized without complex imaging algorithm.

The invention discloses an establishment method of a cherry rootstock Gisela 6 leaf in-vitro regeneration system and transgenic application thereof, and belongs to the field of plant biotechnology and genetic engineering. According to the regeneration system established by optimizing the formula of the culture medium, the regeneration frequency of the 'Gisela 6' leaf is stabilized at a relatively high level, and the quality of the regenerated material is remarkably improved. The callus generated by induction of the method provided by the invention is compact and strong in browning resistance, and the transgenic callus capable of stably expressing RUBY is obtained by a leaf disc transformation method; meanwhile, vitrification of regenerated buds is effectively inhibited, robust plants easy to transplant are obtained, and technical support is provided for precise improvement and breeding speed.

The invention discloses debaryomyces hansenii with high yield of umami peptide and application of the debaryomyces hansenii in improving the umami of low-salt fermented sausages, the debaryomyces hansenii is separated from the anglerkang sausages, is preserved in the China General Microbiological Culture Collection Center on September 30, 2025, and has the preservation number of CGMCC (China General Microbiological Culture Collection Center), and the preservation number of the debaryomyces hansenii HYYAKS044 is CGMCC NO. The biological preservation number is CGMCC (China General Microbiological Culture Collection Center) The content of peptides below 3kDa of the sausage inoculated with the debaryomyces hansenii disclosed by the invention is obviously more than that of a non-inoculated group, and six umami peptides, namely DDHFLFDKP, DPTPSDGKL, FPPDVGGN, ISEQFTAMFR, PETPPP and VDNPGHPFI, can be screened from the peptides below 3kDa, and the six umami peptides are respectively DDHFLFDKP, DPTPSDGKL, FPPDVGGN, ISEQFTAMFR, PETPPP and VDNPGHPFI.

The utility model provides a moving contact system and a circuit breaker comprising the same. The moving contact system comprises a plurality of contact fingers which are arranged in parallel; the contact supporting piece comprises a supporting plate and a pair of supporting arms arranged on the two sides of the supporting plate, and the first ends of the plurality of contact fingers are pivotally installed between the pair of supporting arms; the plurality of springs are arranged corresponding to the plurality of contact fingers and are positioned between the plurality of contact fingers and the supporting plate of the contact supporting piece; and the insulating grating comprises a connecting part which is arranged between the contact finger and the spring. According to the moving contact system, the insulating grating is arranged between the contact finger and the corresponding spring, so that the spring can provide larger contact pressure for overcoming electro-dynamic repulsive force during short-time tolerance, the tolerance performance is improved, meanwhile, the insulating grating and the contact finger can keep synchronous movement, friction force is reduced in the switching-on / switching-off process, and the service life of the moving contact system is prolonged. And the mechanical performance of the circuit breaker is improved.

The utility model discloses a kind of metal intelligent chip card packaging structures, including card base and chip module connected on card base, the card base at least includes upper structural layer, antenna layer and lower structural layer which are thin sheet and are sequentially laminated, the antenna layer is equipped with radio frequency antenna, the radio frequency antenna has at least two conductive contact parts, the card base is equipped with the mounting groove for accommodating chip module in the upper of conductive contact part, the mounting groove groove bottom is equipped with the conductive connection groove extending to conductive contact part downwards, the chip module bottom has the chip contact exposed after packaging, the conductive connection groove is equipped with the conductive material column in the lower part and conductive contact part conductive connection, upper part protrudes upwards and is connected with chip contact conductive connection, the utility model aims at overcoming the deficiency of prior art, provide a kind of metal intelligent chip card packaging structure with compact structure, strong stability.

This invention discloses a method for preparing sulfonamide-modified benzo[a]fluorene-5-one derivatives, using 1,7-enyne and N Using sulfonylaminopyridine salts as raw materials, a series of sulfonated benzo[a]fluorene-5-one compounds were successfully synthesized by catalyzing the sulfonation / cyclization tandem reaction of 1,7-enynes under photocatalytic conditions. This method has excellent functional group tolerance and high economic applicability.

The invention belongs to the technical field of power transformer insulation design and verification, and particularly relates to a power transformer insulation characteristic data analysis and performance prediction method and system, and the method comprises the steps: constructing a structured sample set containing multi-dimensional design parameters and lightning impulse response tags based on a three-phase differential prototype; identifying key variables in the structured sample set and extracting physical consistency constraints; and inputting the key variable and the physical consistency constraint into a preset XGBoost prediction model, and outputting a prediction value and a confidence interval. According to the method, the influence of multi-dimensional parameters on the insulation impact indexes is disassembled into clear main effects and interaction effects, and a traceable mechanism map is formed. Compared with a traditional method which depends on single-point experience judgment or post trial and error, the method has the advantages that standardized data evidences and explainable output are provided, and designers can conveniently and quickly position key constraints, formulate optimization priorities and directly backfill results to design specification and parameter boundaries.

This invention relates to a quadrilateral photonic crystal fiber filter based on dual-wavelength filtering, belonging to the field of fiber optics and photonic device technology. It includes a substrate and a fiber core disposed within the substrate, and a cladding surrounding the fiber core. The cladding contains multiple layers of air holes extending along the fiber axis, arranged periodically in a two-dimensional quadrilateral lattice. A metal thin film is disposed within the cladding to excite surface plasmon resonance (SPR). At least one non-circular air hole is also disposed within the cladding to enhance the birefringence of the fiber. The multiple air holes, metal thin film, and non-circular air holes enable the filter to selectively filter orthogonal polarization states in two different communication bands. This invention, based on SPR and employing a quadrilateral lattice structure, achieves polarization-selective filtering in two communication bands and exhibits good tolerance to manufacturing errors; simultaneously, its regular structure makes it easy to manufacture.

The invention particularly relates to a three-dimensional C arm and optical navigation fused spine screw placement path planning system, and relates to the technical field of intelligent medical treatment and surgical navigation. According to the system, a first coordinate set of coding characteristics in a C-arm image and a second coordinate set of optical navigation tracking are synchronously obtained through a reference device installed on a cone; respectively constructing a spatial topology network based on the image and the navigation coordinate system, and automatically establishing a mapping relation between the image and the navigation coordinate system by using an image matching algorithm to realize fusion registration; the planned path is converted to be stored in a dynamic coordinate system with the device as the reference; during operation, the dynamic coordinate system pose is tracked and solved in real time, and a path is mapped and displayed to guide instrument operation. According to the method, automatic and robust registration without manual marking is realized, and registration failure caused by movement of a patient in an operation is avoided through binding of a path and a dynamic coordinate system.

The invention relates to the technical field of gene editing, and discloses an sgRNA activity prediction method and device based on deep features and automatic machine learning. The method comprises the following steps: acquiring sgRNA training data with editing efficiency labels and discretizing the sgRNA training data into classification labels; after the sgRNA and the PAM sequence are spliced, inputting the spliced sgRNA and PAM sequence into a pre-training depth sequence model to extract high-dimensional features; obtaining a low-dimensional feature vector through nonlinear dimensionality reduction; an automatic machine learning framework is combined with cross validation, and a prediction model is automatically trained and optimized based on low-dimensional features; and finally, carrying out activity prediction and sorting screening on candidate sgRNA by utilizing the model. According to the method, through fusion of deep semantic extraction, nonlinear structure maintenance and a full-automatic modeling process, the accuracy, robustness and development efficiency of sgRNA activity prediction are effectively improved, and an efficient pilot screening tool is provided for a gene editing experiment.

The present application relates to the technical field of fine chemicals synthesis, and discloses a method for high-selectivity co-production of phthaloyl chloride and benzoyl chloride. The method uses dimethylbenzene and trichlorobenzene as raw materials, and feeds them into a photochlorination reaction tower to heat the materials. Then, the reaction tower is kept in a micro-negative pressure condition to perform photochlorination reaction. After the reaction is completed, the liquid is directly fed into a reaction kettle, and phthalic acid and a catalyst are added. Finally, the reaction liquid is separated by rectification to obtain the products phthaloyl chloride and benzoyl chloride. The present application greatly improves the selectivity of dimethylbenzene photochlorination reaction, and inhibits the ring chlorination side reaction. The present application can realize flexible co-production of m-phthaloyl chloride and p-phthaloyl chloride and benzoyl chloride, and can flexibly adjust the generation ratio of phthaloyl chloride and benzoyl chloride according to the raw material supply and product demand, thereby greatly improving the production flexibility of the reaction device.

This disclosure relates to a compound composition comprising stearic acid and moclobemide, which can effectively improve the drought resistance of crops and promote crop growth and development under drought stress. This disclosure also provides uses of the composition and methods for improving crop growth and development under drought stress.

The invention provides a synthesis method of a quaternary cycloalkane modified O-glucoside compound shown in formula 3, in the presence of a catalyst, a sugar compound shown in formula 1 with exposed hydroxyl and a quaternary tension ring substrate compound shown in formula 2 are subjected to nucleophilic addition reaction in an organic solvent to prepare the quaternary cycloalkane modified O-glucoside compound shown in formula 3: X is H, CF3 or Cl; a is a dioxolane type compound or a pyran type compound; the catalyst is phosphazene base P4-t-Bu, and the specific structural formula is shown in the specification.

The present application relates to the field of pharmaceutical preparation and nanomedicine, and particularly relates to a kind of nano-drug composition, its combined drug delivery system and application.The nano-drug composition comprises alpha 1-AR blocker and amphiphilic nano-carrier;The alpha 1-AR blocker is loaded in the hydrophobic core formed by amphiphilic nano-carrier;The alpha 1-AR blocker is selected from one or more of prazosin, terazosin, doxazosin;The average particle size of the nano-drug composition is 220-260 nm, the polydispersity index is less than 0.3, and the encapsulation efficiency is greater than 50%.The nano-drug composition of the present application has dual functions of anti-tumor proliferation and immune microenvironment remodeling, improves the dispersion stability of the drug in physiological medium, effectively reduces the in vivo clearance rate, prolongs the circulation time, and realizes the efficient in vivo delivery of the drug.The preparation process of the nano-preparation is stable and can be produced on a large scale.

The invention discloses a primer and a kit for rapidly detecting yersinia rouxii through RPA-CRISPR / Cas12a and application of the primer and the kit, and relates to the technical field of disease prevention and control in aquaculture. The invention aims to solve the problems that the existing detection method is time-consuming and labor-consuming, is easy to cause pollution and needs to be matched with professional detection equipment. According to the present invention, the RPA amplification primer is composed of a forward primer yrp1-RPA-F2 and a reverse primer yrp1-RPA-R2, the crRNA is yrp1-crRNA-R1, and the kit for rapid detection of yersinia rouxii contains the RPA amplification primer and the crRNA. The method for rapidly detecting yersinia rouxii comprises a fluorescence method and a test paper method. The method disclosed by the invention has the advantages of good inclusiveness, strong specificity, high sensitivity and the like, detection is carried out based on the RPA primer and the crRNA, and the obtained RPA product is detected by using a lateral flow chromatography test strip and fluorescence, so that the purpose of portable visual rapid detection on site or in a breeding base is achieved, and the method is suitable for large-scale popularization and application. The method has very high clinical application value in the aspects of early discovery of diseases, epidemiological investigation and comprehensive prevention and control.

This invention belongs to the field of microbial pesticides and discloses a highly effective Bacillus subtilis strain for killing spider mites and its applications. The Bacillus subtilis strain described in this invention has the preservation number CCTCC NO: M 20251959. Indoor bioassay data show that Bacillus subtilis NBIF-051 has high toxicity against major agricultural mites such as the two-spotted spider mite, the citrus red spider mite, and the cotton red spider mite. Pot experiments showed that after spraying the fermented broth of NBIF-051 for 7 days, the control efficacy against the two-spotted spider mite was 65.18%. Field trials showed that after spraying the fermented broth of this strain for 15 days, the control efficacy against the citrus red spider mite was 63.45%~76.85%, comparable to commercially available chemical acaricides. Furthermore, the acaricidal activity of the fermented broth of this strain exhibits certain tolerance to high temperatures and ultraviolet radiation, showing broad application prospects in agricultural production.

This application discloses a precision capacitance sensing system, comprising a modulation signal source, a sensitive probe, a differential transformer bridge, a signal amplification link, an analog multiplier, and a low-pass filter circuit, all connected in sequence. It also includes a demodulation reference signal source, the output of which is connected to the second input of the analog multiplier. The demodulation reference signal source is used to generate a multi-frequency combination demodulation reference signal, which is composed of a fundamental frequency component and at least two harmonic components superimposed. This invention also discloses a precision capacitance sensing method. By employing a specially designed demodulation reference signal, this application ensures that the system output remains constant within a continuously varying range of the demodulation phase difference, thereby achieving complete insensitivity to phase difference fluctuations and effectively overcoming the performance bottleneck that has long constrained high-precision capacitance sensing.

This invention discloses an acid and alkali resistant polyamide membrane and its preparation method. The method is as follows: S1: Immerse the ultrafiltration base membrane in an aqueous sodium hydroxide solution for soaking and then set aside; S2: Treat the base membrane with a mixed aqueous solution of polyamine and aziridine crosslinking agent; S3: Wet the base membrane in an organic phase solution of polyacrylamide monomer to obtain a polyamide composite membrane; S4: Immerse the prepared polyamide composite membrane in an alcoholic solution of hydroxymethyl crosslinking agent, then dry it in a forced-air drying oven, and after washing with water, obtain the acid and alkali resistant polyamide membrane. This application uses two crosslinking agents, aziridine and hydroxymethyl, which can undergo specific crosslinking reactions with the carboxyl groups inside the polyamide membrane, to directly perform dual crosslinking modification on the membrane interior and surface, without introducing other polymer materials to avoid excessive increase in membrane permeation resistance. The polyamide membrane prepared by this method has both good acid and alkali resistance and water permeability.

The application relates to a translational tolerance connector and a connector seat, the connector seat comprising a base with a fixed outer conductor, a floating seat composed of a floating insulator and a floating outer conductor arranged inside and outside, and an inner conductor, the floating seat as a whole is placed in the fixed outer conductor through the elastic bending part of the inner conductor, the base is provided with clamping parts for restricting the floating seat as a whole to horizontally slide along the X and Y directions from the upper and lower sides, and the floating outer conductor is provided with sliding parts in elastic abutment with the clamping parts, so that the floating outer conductor and the fixed outer conductor are in elastic abutment during the horizontal sliding of the floating seat along the X and Y directions. The application aims to provide a translational tolerance connector and a connector seat with excellent shielding effect and tolerance performance.

The invention relates to the technical field of skin care, and discloses a sustained-release composition containing azelaic acid composite lipidosome and application of the sustained-release composition in acne treatment and skin repair. Comprising a composite liposome, an encapsulated active component and a dispersion medium, the composite liposome is composed of a lipid bilayer, the lipid bilayer comprises phospholipid, cholesterol and pegylated phospholipid, and the phospholipid is a mixture of hydrogenated soybean phospholipid and dipalmitoyl phosphatidylcholine; the encapsulated active component comprises a fat-soluble component and a water-soluble component; the water-soluble components comprise a hamamelis virginiana leaf water extract and a herba portulacae extract; the dispersion medium is a phosphate buffer solution; by optimizing lipid composition, introducing surface modification and constructing a multi-layer release system, the invention aims to synergistically exert multiple effects of antibiosis, anti-inflammation, cutin regulation, red fading, repair and the like, and provides an efficient, mild and integrated solution for acne and sequelae skin problems thereof.

The application belongs to the technical field of adhesives and sealants, and specifically provides a sealing wax as well as a preparation method and application thereof. The sealing wax comprises the following components in parts by mass: microcrystalline wax 40-60 parts, polyethylene wax 1-5 parts, ethylene-vinyl acetate copolymer 3-8 parts, low-molecular-weight polyisobutylene 2-5 parts, medium-molecular-weight polyisobutylene 2-5 parts, tackifying resin 15-20 parts, and flame retardant 3-5 parts. The number-average molecular weight of the low-molecular-weight polyisobutylene is less than 5000, and the viscosity-average molecular weight of the medium-molecular-weight polyisobutylene is 10000-40000. Through the design and mutual compounding of the components, the sealing wax has a high melting point, a low water absorption rate, a high volume resistivity, good high-temperature and low-temperature resistance, and strong waterproof and moisture-proof performance, high mechanical strength and toughness, high insulation, high transparency, and strong sealing effect, and has good flow leveling property and good flame retardant performance, and can meet the strict use requirements of high-end electronic equipment.

This invention provides a method for identifying the authenticity of wheat flour based on the fusion of Raman and near-infrared spectroscopy, belonging to the field of wheat flour authenticity identification technology. The method includes: acquiring Raman and near-infrared spectral data of the wheat flour sample to be tested, and preprocessing them separately; constructing and training a fusion detection model, which includes a data-level fusion module, a feature-level fusion module, and a decision-level fusion module; the data-level fusion module generates full-spectrum fusion data; the feature-level fusion module concatenates core features into a comprehensive feature set; the decision-level fusion module inputs the comprehensive feature set into a hybrid model; and the trained fusion detection model processes the comprehensive feature set to output the authenticity identification result of the wheat flour sample to be tested. This invention, through a three-level spectral fusion architecture and a dynamic adaptive adversarial mechanism, can effectively achieve high-precision and high-robust identification of wheat flour authenticity.

The application relates to the field of biomedical technology, and discloses an application of a GCA-NAb monoclonal antibody in the preparation of a drug for preventing and / or treating pulmonary fibrosis. The application mainly relates to the application of an amino acid sequence of the GCA-NAb monoclonal antibody as shown in SEQ ID No. 2 in the preparation of the drug for preventing and / or treating pulmonary fibrosis. The GCA-NAb monoclonal antibody can significantly improve pulmonary fibrosis, and can prolong the survival cycle of a mouse model, reduce the proportion of TH17 cells in lung tissues, and slow down the progress of pulmonary fibrosis. In addition, the antibody can directly affect the transformation of fibroblasts, inhibit the transformation of the fibroblasts into myofibroblasts, and thus reduce the fibrosis. The GCA-NAb monoclonal antibody has the characteristics of high efficiency and safety.

The invention discloses a three-dimensional packaging chip system with a built-in interconnection test function and a detection method thereof, and the system comprises a packaging semiconductor main body which is internally provided with a plurality of wiring layers, a testability design (DFT) circuit which is integrated in a chip, and an internal multi-channel selection switch network which is controlled by the DFT circuit and is arranged on each wiring layer. And at least two detection pins / pads which are arranged outside the package and have a small number and increased pin spacing. According to the method, the DFT circuit is used for controlling switching of the switching network, different internal interconnection paths are multiplexed to the detection point in a time division mode for electrical measurement, and accurate positioning of fault nodes is achieved by analyzing multiple sets of measurement results. According to the method, the problem that the needle card is difficult to test due to warping of high-density packaging is effectively solved, hierarchical and nodal accurate diagnosis of internal interconnection faults of three-dimensional packaging is realized for the first time, the test cost and complexity are remarkably reduced, and a hardware basis is provided for online function repair.

A low-density, high-stability fluorinated polyimide aerogel is prepared by using TFMB, ODA, and 6FDA as raw materials to prepare a polyamic acid solution. Triethylamine is added to the polyamic acid solution to prepare a fluorinated polyamic acid salt. The fluorinated polyamic acid salt is dissolved in a mixture of water and diphenyl ether, and then triethylamine and N,N-dimethylcyclohexylamine are added to prepare a polyamic acid hydrogel. The hydrogel is then freeze-dried to obtain the polyamic acid aerogel, which is finally subjected to thermal imidization treatment. This invention uses three monomers to form an aerogel, creating a low-density, high-porosity three-dimensional network structure. This structure is stable, has a high heat resistance temperature, and maintains good performance at high temperatures. The aerogel also possesses a certain degree of rigidity and strength, making it resistant to deformation and structural collapse under external forces.