The invention relates to a
gene GmHB1a participating in development of vascular bundles of soybean root nodules and application of the
gene GmHB1a. Specifically, the invention relates to a separated
gene participating in development of vascular bundles of soybean root nodules or regulation and control of the number of soybean nodules, and a
coding region sequence of the gene is shown as SEQ ID NO: 1 or a
homologous sequence of the gene. The invention also provides a method for regulating and controlling the nodulation number of the
plant, which comprises the following steps: introducing the gene or a sequence for inhibiting the expression of the gene into a target
plant cell or tissue to obtain a transgenic
plant, the nodulation number of the transgenic plant is changed compared with that of the target plant, the plant is a leguminous plant, preferably soybean, and the nodulation number of the transgenic plant is changed. Optionally, the change in number of nodules is manifested as an increase in number of nodules and / or in number of single nodules, and optionally, the change in number of nodules is manifested as a decrease in number of nodules and / or in number of single nodules.