1515 results about "Transgene" patented technology

The invention discloses protease for improving the resistance of potatoes to late blight as well as a coding gene and application thereof, and belongs to the technical field of genetic engineering and plant disease resistance breeding. The amino acid sequence of the protease StRD21 is as shown in SEQ ID NO.2, or the protease StRD21 has a derivative sequence with the same disease-resistant function. The nucleotide sequence of the coding gene is as shown in SEQ ID NO. 1. The invention also provides a specific primer pair for cloning the gene, a potato recombinant overexpression vector containing the gene and a transgenic host cell. The StRD21 gene is introduced into potatoes and overexpressed, so that the resistance of the potatoes to late blight can be remarkably enhanced. The invention provides an effective gene resource and a biotechnological means for cultivating a new variety of disease-resistant potatoes.

The invention discloses application of a GsRZ1A gene in improving alkali resistance of plants, and belongs to the technical field of genetic engineering. The nucleotide sequence of the GsRZ1A gene is as shown in SEQ ID NO: 1, and the coded amino acid sequence of the GsRZ1A gene is as shown in SEQ ID NO: 2. A transgenic line is obtained by constructing an overexpression vector of the GsRZ1A gene and transforming soybeans. A transgenic line is subjected to alkali stress treatment, and a result shows that the overexpressed GsRZ1A soybean line shows a faster relative growth rate, the total leaf area, the leaf chlorophyll content and the relative water content are all higher than those of a control line of an empty vector, the total root length and the total root surface area of a root system are also remarkably larger than those of a control group, and the antioxidant enzyme activity can be improved; the accumulation of superoxide anions and malonaldehyde is reduced, and the accumulation of osmotic regulation substances such as soluble sugar is increased to enhance the tolerance of the soybeans to alkali stress, so that the method has an important application prospect in alkali-resistant plant breeding.

The invention belongs to the technical field of molecular biology and genetic engineering, and particularly relates to a passion fruit PeDREB1 gene and application thereof in regulating temperature and drought stress tolerance. By overexpressing the passion fruit PeDREB1 transcription factor in the plant, the tolerance of the transgenic plant to temperature stress (low temperature and high temperature) and drought stress can be obviously enhanced, the growth recovery capability of the transgenic arabidopsis thaliana overexpressed with PeDREB1 after low temperature stress is obviously superior to that of a wild type, and the leaf area growth rate is increased by about 0.45-2.28%; under the condition that 0.2 mol / L to 0.3 mol / L mannitol simulates drought stress, the seed germination rate is increased by 5.39 percent to 9.29 percent, and the root length growth rate is increased by 1.08 percent to 6.72 percent; the invention provides a clear gene target and a directly applicable molecular resource for plant stress resistance molecular breeding, can provide theoretical support for subsequent clarification of a regulation mechanism of plant response temperature and drought stress, and also lays a foundation for plant stress resistance genetic improvement and new germplasm creation.

A soybean cultivar designated 28310330 is disclosed. The invention relates to the seeds of soybean cultivar 28310330, to the plants of soybean cultivar 28310330, to the plant parts of soybean cultivar 28310330, and to methods for producing progeny of soybean cultivar 28310330. The invention also relates to methods for producing a soybean plant containing in its genetic material one or more transgenes and to the transgenic soybean plants and plant parts produced by those methods. The invention also relates to soybean cultivars or breeding cultivars, and plant parts derived from soybean cultivar 28310330. The invention also relates to methods for producing other soybean cultivars, lines, or plant parts derived from soybean cultivar 28310330, and to the soybean plants, varieties, and their parts derived from use of those methods. The invention further relates to hybrid soybean seeds, plants, and plant parts produced by crossing cultivar 28310330 with another soybean cultivar.

The invention discloses an upland cotton GhCRK29 gene as well as an encoding protein and application thereof, the GhCRK29 gene is separated from cotton, the nucleotide sequence of the GhCRK29 gene is as shown in SEQ ID No.1, and the amino acid sequence of the encoding protein of the GhCRK29 gene is as shown in SEQ ID No.2. According to the invention, a virus-induced gene silencing technology and a transgenic overexpression technology are further utilized to carry out preliminary research on the function of the cotton GhCRK29 gene in cotton verticillium wilt resistance, and a result shows that the verticillium wilt resistance of a cotton seedling plant is obviously reduced after the GhCRK29 gene in cotton is silenced; after the GhCRK29 gene is over-expressed in arabidopsis thaliana or cotton, the verticillium wilt resistance of arabidopsis thaliana or cotton is remarkably improved, and the resistance of a GhCRK29 gene forward regulation plant to verticillium dahliae is determined; the invention has application prospects in the aspects of improving the verticillium wilt resistance of plants or cultivating verticillium wilt resistant plant varieties and the like.

The invention discloses an EsSTB1 gene capable of increasing the number of branches of a plant, reducing the plant height and delaying flowering. The EsSTB1 gene has the functions of increasing the number of branches, reducing the plant height and delaying flowering, and a scientific basis and a new material are provided for cooperative regulation and control of tillering formation and reproductive growth of siberian wildrye; besides, the functional verification method of the EsSTB1 gene provided by the invention performs functional verification by applying transgenic arabidopsis thaliana starting from an internal regulation mechanism of tillering regulation of siberian wildrye, has obvious research depth and breadth and technical effects, and can obtain a plant strain with an intentional phenotype. Through verification, the siberian wildrye EsSTB1 gene disclosed by the invention can be used for remarkably increasing the number of branches of a plant, reducing the plant height and delaying flowering. The method is suitable for popularization and application in the biotechnology field.

The application belongs to the technical field of genetic engineering, and provides application of a PagLBD16.2 gene in regulating development of a poplar plant type, wherein a nucleotide sequence of the PagLBD16.2 gene is shown as SEQ ID NO. 3. The regulation is achieved by overexpressing the PagLBD16.2 gene, so that the plant height of a transgenic poplar is lower than that of a wild type 84K, the internode diameter is lower than that of the wild type 84K, and the number of internodes is more than that of the wild type 84K. The application has important significance for promoting genetic improvement and breeding of the poplar by overexpressing the poplar gene PagLBD16.2 to regulate development of the poplar plant type.

The invention discloses application of rice protein MBS and a coding gene thereof in plant light protection. The invention provides any one of the following applications of the protein OsMBS. C1, regulating and controlling the plant yield; c2, regulating and controlling light protection of the plants; c3, regulating and controlling the strong light adaptability of the plants; c4, regulating and controlling the photosynthetic activity of the plants; and C5, cultivating a strong light resistant plant. Transgenosis experiments and field yield measurement verify that the homologous protein OsMBS has enhanced expression in rice, the chloroplast is wrapped in a sharp increase mode under strong light, a shading layer for directly protecting the chloroplast is formed, light protection of the rice fragrance of the rice can be enhanced, and remarkable and efficient yield increase in the field is achieved.

The invention provides application of a tobacco NtMYC1 gene in promoting growth and development of tobacco glandular hair, and belongs to the technical field of plant breeding. The tobacco MYC1 transcription factor is obtained by carrying out sequence characteristic analysis, conservative structural domain identification, protein physicochemical property analysis and phylogenetic analysis on a genome of tobacco. And an RNAi technology and an overexpression tobacco transgenic line verify that the tobacco MYC1 transcription factor and the coding gene thereof have the effect of regulating and controlling the growth and development of the tobacco glandular hair, and the growth and development of the tobacco glandular hair are promoted through forward regulation and control, so that the growth and development of the tobacco glandular hair are inhibited through silent expression. Therefore, the tobacco MYC1 transcription factor and the coding gene thereof provided by the invention provide a new means for tobacco breeding.

A soybean cultivar designated 37050315 is disclosed. The invention relates to the seeds of soybean cultivar 37050315, to the plants of soybean cultivar 37050315, to the plant parts of soybean cultivar 37050315, and to methods for producing progeny of soybean cultivar 37050315. The invention also relates to methods for producing a soybean plant containing in its genetic material one or more transgenes and to the transgenic soybean plants and plant parts produced by those methods. The invention also relates to soybean cultivars or breeding cultivars, and plant parts derived from soybean cultivar 37050315. The invention also relates to methods for producing other soybean cultivars, lines, or plant parts derived from soybean cultivar 37050315, and to the soybean plants, varieties, and their parts derived from use of those methods. The invention further relates to hybrid soybean seeds, plants, and plant parts produced by crossing cultivar 37050315 with another soybean cultivar.

The invention relates to the technical field of biological genetic engineering, in particular to application of a brassica napus BnaA01G0331000ZS gene in drought stress resistance. The BnaA01G0331000ZS gene is cloned by using cDNA of double 11 leaves as a template through a sequence published in a brassica napus genome database, an over-expression vector is constructed and genetically transformed into rape variety high oil for transgenic function verification, the drought resistance of the obtained over-expression transgenic line is obviously enhanced compared with that of a wild type, and the BnaA01G0331000ZS gene has the advantages that the BnaA01G0331000ZS gene is obtained; therefore, the gene can positively regulate the drought stress resistance of the rape. Therefore, the invention provides a new gene resource for adversity stress resistance of rape.

The present invention is directed to a modified HSV-1 vector comprising at least two transgenes that exhibit controllable and distinct kinetics of expression, a pharmaceutical composition thereof suitable for use therapeutically or for in vitro applications, and methods relating thereto.

The present disclosure relates to novel adeno-associated adenovirus (AAV) vectors comprising targeting peptides. More particularly, the present disclosure relates to an adeno-associated serotype 2 virus vector, AAV2, comprising a transgene encoding LIGHT wherein the viral capsid of the AAV2 vector comprises a targeting peptide that alters its tropism to target tumor endothelial cells; and a use of the carrier. The disclosure also relates to the use of said vectors in therapy, in particular in the treatment of cancer.

Disclosed herein is a method for producing a lipid or oil in a plant, the method comprising genetically modifying the plant to express a plurality of heterologous proteins selected from PaWRI1, PaWRI2, PaDGAT1, or PaPDAT1, or variants thereof. The expression of the plurality of heterologous proteins in the genetically modified plant may result in a change in the nutrient profile of the plant relative to non-genetically modified plants of the same species. Also disclosed herein is a method for producing fatty acid and triacylglycerol content in plant non-seed tissue. Further disclosed herein is a method for producing a genetically modified plant.

The invention belongs to the technical field of gene engineering, and particularly relates to application of corn zma-miR319 and / or a target gene of the corn zma-miR319 in regulation and control of corn stem rot resistance. According to the application, sequencing data of small RNA, a degradation group and a transcriptome are integrated, a miRNA family member zma-miR319 is identified, and the resistance of corn to stem rot can be positively regulated and enhanced; the target gene ZmMYB74 is a core regulation gene of an Fg response module, the stem rot resistance of a transgenic plant over-expressing the ZmMYB74 is weakened, and lignin deposition is reduced; the resistance is obviously enhanced by knocking out or inhibiting ZmMYB74, the ZmMYB74 is used as a transcription inhibition factor, the expression of a lignin synthesis related gene ZmCAD is negatively regulated and controlled, and the separation of the disease-resistant gene ZmMYB74 not only contributes to the cultivation of a persistent disease-resistant corn variety, but also deepens the understanding of a stem rot resistance molecular mechanism.

Plants, cells, tissues, and germplasms comprising genes and labeled alleles associated with increased soybean cyst nematode (SCN) resistance are provided. Also provided are methods of breeding plants having alleles associated with increased SCN resistance and methods of identifying and selecting plants having alleles associated with increased SCN resistance. Transgenes and genome-edited plants comprising gene alleles associated with increased SCN resistance are provided.

The invention belongs to the technical field of biology, and particularly relates to a cassava MeTGA9 gene and application thereof. The nucleotide sequence of the cassava MeTGA9 gene is as shown in SEQ ID NO: 1. The cassava MeTGA9 gene is over-expressed in cassava, so that the plant height and the root length of the transgenic cassava can be remarkably reduced. The MeTGA9 gene provided by the invention has important significance in the aspects of cassava genetic improvement, cultivation of new cassava dwarf varieties, cultivation of new cassava varieties suitable for interplanting and the like, and provides valuable gene resources and theoretical basis for cultivation of new cassava varieties with excellent plant types.

The invention discloses a recombinant adeno-associated virus vector for targeted delivery of VEGF-C (vascular endothelial growth factor-C) and application of the recombinant adeno-associated virus vector in promoting endocardial differentiation to treat coronary heart disease. The rAAV vector comprises transgene for coding VEGF-C protein or VEGF-C protein functional variant and a tissue-specific promoter operably connected to the transgene, wherein the tissue specific promoter is capable of driving the transgene to be specifically expressed in the heart endothelial cells. According to the treatment method developed by the invention, specific expression of the Vegfc gene in the heart is driven by using an ICAM2 promoter with targeting to endothelial cells, and a remarkable curative effect is shown in a myocardial infarction model mouse. The therapeutic scheme provides a new effective strategy for intervention and treatment of myocardial infarction and chronic heart failure, and has the advantages of high safety, long gene expression duration, extremely low immune response to a host and the like.

The invention relates to the field of agricultural bioengineering, in particular to application of an oilseed rape BnaA08G0167600WE gene in enhancing pod shattering resistance of oilseed rape. According to the invention, after a transgenic plant is constructed in oilseed rape by utilizing a modern biotechnological means and the oilseed rape gene BnaA08G0167600WE is knocked out, the silique of the mutant is not easy to crack compared with a wild type under the action of external force, and a separation layer and a wood layer of the cross section of the silique of the mutant have more cell connections compared with the wild type, so that the yield of the mutant is improved. And the generation of the mutants provides an important genetic material for further researching the function of the gene and the effect of the gene in the rape.

The invention relates to the field of gene engineering, in particular to Os HSL mutants and coding genes, expression vectors, transgenic cells, compositions and application thereof, the mutants are Os HSL1 enzyme and / or Os HSL2 enzyme mutants, the amino acid sequence of the Os HSL1 enzyme is as shown in SEQ ID NO.1, and the amino acid sequence of the Os HSL2 enzyme is as shown in SEQ ID NO.2. The invention further relates to an application of the Os HSL mutants and the coding genes, the expression vectors, the transgenic cells and the compositions. The invention relates to a gene, a protein, a carrier, a cell and a composition which endow plants with a detoxification effect on an HPPD inhibitor and application of the gene, the protein, the carrier, the cell and the composition to plant improvement, so that the plants are endowed with resistance to the HPPD inhibitor.

The invention discloses an alfalfa growth period and quality related protein MsCOG1 as well as a coding gene and application thereof. The Dof transcription factor is separated from medicago sativa, the amino acid sequence of the Dof transcription factor is as shown in SEQ ID No.2, and the nucleotide sequence of the coding gene of the Dof transcription factor is as shown in SEQ ID No.1. According to the invention, the coding gene of the Dof transcription factor in medicago sativa is over-expressed in medicago sativa, so that compared with a wild type, the obtained transgenic medicago sativa material has the advantages that the flowering time is obviously delayed, the contents of nutritional ingredients such as crude protein and trace elements are obviously increased, and the contents of acid detergent fibers, neutral detergent fibers and lignin are obviously reduced; the relative feeding value and the relative feeding quality are obviously improved. The invention provides a gene resource for molecular breeding and genetic engineering improvement of forage grass, and has application prospects in the aspects of cultivating high-quality and high-yield forage grass varieties or controlling the flowering period of the forage grass, improving the quality of the forage grass and the like.

The present application belongs to the technical field of genetic engineering, and particularly relates to a methyltransferase gene CmCMT2 and CmDRM2 application in regulating chrysanthemum flowering. The present application constructs an RNAi silencing expression vector by isolating CmCMT2 and CmDRM2 genes in a chrysanthemum variety "Jinma", and obtains CmCMT2 -RNAi and CmDRM2 -RNAi transgenic "Jinma" plants by using a genetic transformation method, then performs phenotype observation and statistics on the transgenic plants, and explores CmCMT2 and CmDRM2 the mechanism of the genes affecting early flowering of chrysanthemum by using an RNA-seq method. The CmCMT2 and CmDRM2 genes are cloned from the chrysanthemum variety "Jinma", and spatiotemporal expression analysis shows that CmCMT2 and CmDRM2 the genes are highly expressed during the process of "Jinma" from bud development to color change; and CmCMT2 and CmDRM2 the expression level in leaves gradually decreases during the growth and development of "Jinma". The research results show that CmCMT2 and CmDRM2 the content of GA1 is changed by negatively regulating the expression of CmG20ox2a and CmG20ox2b , so that the flowering period of chrysanthemum is advanced by 15 and 8 days, respectively.

The present invention provides an adeno-associated virus (AAV) vector gene therapy for use in treating a monogenic form of nephrotic syndrome, wherein the AAV vector comprises a NS-associated transgene and minimal nephrin promoter NPHIS1 or podocin promoter NPHIS2.

The invention belongs to the technical field of biotechnology, and relates to a wheat drought-resistant related protein TaELF4 as well as a coding gene and application thereof. A recombinant vector p110UBI-R containing a TaELF4 gene CDS sequence of SEQ ID NO.1 is used for transforming wheat, the survival rate of an obtained T3-generation transgenic line is remarkably higher than that of wild type wheat under the drought condition, the yield is averagely increased by about 15%, and the yield is statistically remarkably higher than that of the wild type wheat. The invention has important significance in molecular breeding for regulating and controlling the drought resistance and yield of wheat.

The invention relates to haptoglobin or a nucleic acid encoding haptoglobin for use in treating or preventing of exaggerated erectile response and / or preventing permanent erectile dysfunction. In addition, the invention relates a pharmaceutical composition for use in treating or preventing exaggerated erectile response and / or preventing permanent erectile dysfunction, wherein the pharmaceutical composition comprises an adeno-associated viral (AAV) vector with a transgene encoding a haptoglobin gene. The exaggerated erectile response may be priapism, for example priapism associated with sickle cell disease (SCD).

The invention provides application of a potato StMYB4 gene in regulation and control of solanine accumulation in potato tubers, the StMYB4 gene is cloned through a sequence published in a potato genome database by taking cDNA of leaves of cultivated species Hubei potato No.3 and AC142 as a template, an overexpression and knockout vector is constructed, genetic transformation is performed on the Hubei potato No.3 and AC142 respectively for transgenic function verification, and the solanine accumulation in the potato tubers is regulated and controlled. It is found that excessive StMYB4 genes can significantly promote synthesis of solanine in potato tubers, and knockout of the StMYB4 genes can significantly inhibit synthesis of solanine in potato tubers. The application of the potato StMYB4 gene to regulation and control of tuber solanine can be used for molecular marker development and low solanine variety breeding.

The application discloses application of an OSA3 gene, relates to the technical field of genetic engineering, and the nucleotide sequence of the OSA3 gene is shown as SEQ ID NO. 3. The application research proves that the OSA3 gene has the ability of regulating the deep root ratio of rice, and by using the ability, drought-resistant transgenic rice can be developed for arid regions. Meanwhile, the OSA3 gene knockout mutant osa3-1 / osa3-2 constructed by the application has the increased number of shallow root systems, and can be used for developing transgenic rice resistant to poor soil for poor soil regions, and enhancing the adaptability of rice germplasm to the environment.

The invention discloses an effect of OsCPK18 in inhibition of pre-harvest germination of rice. According to the invention, the Zhonghua 11 is taken as the background, and two mutant plants, namely, KO-OsCPK18 # 1 and KO-OsCPK18 # 2, with OsCPK18 gene function defect mutation are obtained through transgenosis; in a seed germination experiment, it is found that seed germination of the OsCPK18 mutant material is delayed, and the final germination rate is not affected; a pre-harvest sprouting simulation experiment is carried out in a high-temperature and high-humidity environment arranged in an incubator, and the pre-harvest sprouting resistance of the OsCPK18 mutant material is found to be remarkably higher than that of a wild type. The results show that the OsCPK18 gene under the background of ZH11 has important significance on crop breeding, the invention provides a method for improving the pre-harvest sprouting resistance of rice, and the cultivation process of a new pre-harvest sprouting resistant rice variety can be accelerated.

The application discloses a P450 enzyme mutant for degrading herbicides and application thereof, and the P450 enzyme mutant is named P450 BSβ -F46A, P450 BSβ -F46A-CPR; the amino acid sequences are shown in SEQ ID NO. 2 and 4 respectively. The application also discloses application of the P450 enzyme mutant in degrading pyridine oxycarboxylic acid herbicides or aryloxyphenoxypropionic acid herbicides and related genes in cultivating herbicide-resistant transgenic crops. Experiments prove that the highest conversion rate of the P450 enzyme mutant for degrading pyridine oxycarboxylic acid herbicides reaches 86%, and the total conversion number of catalyzing flumiclorac can reach 7443 at most, and the highest catalytic conversion rate of degrading aryloxyphenoxypropionic acid herbicides reaches 89%. The P450 enzyme mutant provided by the application has the advantages of high catalytic efficiency, high protein expression amount and low industrial cost, and the whole operation process for degrading herbicides is simple, the process is mature, the cost is low, and the application prospect is wide.

The present invention relates to nucleic acids and modified immune cells or precursors thereof, comprising a first polynucleotide encoding an NF-κB p65-rTetO fusion protein comprising one or more transactivation domains (TAD) of human NF-κB p65 fused to a reverse Tet operator (rTetO) binding domain; a second polynucleotide comprising a Tet operator region (TetOR) for providing inducible expression of one or more transgene(s) operatively linked thereoto; and a third polynucleotide comprising a promoter directing transcription of p65-rTetO from a first strand of DNA, where the p65-rTetO is configured to induce transcription of a transgene of interest operatively linked to the TetOR in the presence of tetracycline or doxycycline (Dox) from an opposite strand of DNA.