67 results about "Inducible gene" patented technology

The invention belongs to the technical field of plant genetic engineering, and particularly relates to an SCRE10 gene for improving rice disease resistance and application of the SCRE10 gene, the base sequence of the SCRE10 gene is shown as SEQ ID NO.1, and the amino acid sequence of the SCRE10 gene is shown as SEQ ID NO.2. Through construction of a dexamethasone induced expression SCRE10 transgenic rice plant, it is found that the transgenic rice can significantly induce PR gene expression and active oxygen outbreak, and the resistance of rice to false smut and bacterial leaf blight can be improved. The invention proves that heterologous inducible expression of the Ustilaginoidea virens SCRE10 gene has the function of positively regulating the disease resistance of the rice, and the SCRE10 gene can be used for improving the disease resistance of the rice, which is of great significance to the creation of disease-resistant germplasm of the rice.

The invention discloses a verticillium dahliae virulence gene, a verticillium dahliae virulence protein and application. The invention relates to the technical field of biology, and provides a method for preventing and treating cotton verticillium wilt based on RNAi (RNA interference) as well as related components and application thereof. Specifically, the invention discloses application of the verticillium wilt prevention and treatment by a method for inhibiting or silencing the expression of verticillium dahliae pathogenic gene VdHDAC (coding protein of the verticillium dahliae pathogenic gene VdHDAC is shown as SEQ ID NO: 3), the core of the verticillium wihliae pathogenic gene VdHDAC is to provide dsRNA molecules capable of targeting the gene, and the positive-sense strand of the dsRNA molecules is preferably selected from SEQ ID NO: 5, SEQ ID NO: 8, SEQ ID NO: 9 or SEQ ID NO: 11. Based on the molecule, the invention further provides a recombinant vector containing the coding sequence, a recombinant microorganism, a transgenic disease-resistant plant and an RNAi pesticide preparation. The scheme can be realized through host-induced gene silencing, microorganism-mediated gene silencing or preparation treatment and the like, the virulence of pathogenic bacteria can be effectively reduced, and a new technical approach is provided for green prevention and control of verticillium wilt.

The invention relates to the technical field of plant genetic engineering, and particularly provides cloning of a GbGELP25D gene of gossypium barbadense and application of the GbGELP25D gene of gossypium barbadense. According to the invention, the GbGELP25D gene with a full length of 1092 bp is cloned from the sea island cotton variety Xinhai No. 7 for the first time, and the gene encodes a secretory lipase protein with a signal peptide and is positioned in an extracellular gap. Through bioinformatics analysis, phylogenetic classification, protein structure modeling and signal peptide function verification, it is clear that the gene belongs to a plant GELP family. Furthermore, a virus-induced gene silencing technology is utilized to prove that the silent GbGELP25D can obviously enhance the resistance of cotton to verticillium wilt, and the mechanism of the silent GbGELP25D is closely related to activation of ethylene synthesis and signal channels and induction of expression of disease-resistant related genes. The resistance gene provided by the invention enriches gene resources of cotton verticillium wilt resistance breeding, and has important theoretical significance and application value.

The invention discloses a nucleic acid construct for inducing DDB2 gene silencing and application of the nucleic acid construct. The designed targeted expression system based on the adeno-associated virus (AAV) vector is higher in specificity, non-target tissue can be prevented from being interfered, the tissue specificity of gene intervention is improved, and the off-target risk is small; the shRNA has high stability and processing efficiency, reduces immune response and interference effect, has high expression efficiency and strong histocompatibility, and is suitable for long-term expression intervention in vivo.

In one aspect, a method of treating an NK cell to induce a gene edit. The method may include contacting an NK cell with a transfection composition including poly-L-glutamic acid (y- PGA), a single-stranded oligo deoxynucleotide (ssODN) enhancer, or a combination thereof; contacting the NK cell with an editing composition including: a DNA-PK inhibitor, and an HD AC inhibitor; electroporating the NK cell; and contacting the NK cell with a recovery composition including DNase I. In another aspect, a method of preparing an engineered NK cell comprising a fluorescent reporter at an endogenous locus. In another aspect, a method of treating an NK cell to introduce an exogenous nucleic acid.

The invention relates to the technical field of biomedicine, in particular to a novel target spot for resisting chikungunya virus infection and application. According to the invention, human colon cancer cells (HCT116) are taken as target cells, and retinoic acid-induced protein expression of the target cells is reduced by adopting a gene knockout technology, so that host factors capable of effectively inhibiting CHIKV infection of the human colon cancer cells are found, and the purpose of blocking CHIKV infection from the source is achieved. It is found that retinoic acid induced protein 16 (RAI16) plays an important role in CHIKV infected HCT116 cells, and CHIKV infection can be obviously promoted by down-regulating expression of RAI16. The invention provides an application of RAI16 in preparation of a medicine for preventing or treating chikungunya virus infection, and provides a new target spot and a treatment scheme for clinically preventing and treating fever, arthralgia, arthrocele, muscular pain, headache, nausea, fatigue, rash and other diseases caused by CHIKV infection.

The application belongs to the technical field of molecular plant pathology, and particularly relates to a peanut disease-resistant gene AhNHL24 and application thereof. Through whole genome identification and bioinformatics analysis, 45 NHL family genes (AhNHL) are identified in peanuts, and gene expression analysis shows that most of the AhNHL genes can respond to the induction of biological stress and abiotic stress. Gene disease resistance identification finds that transient overexpression of AhNHL14, AhNHL24 and AhNHL31 can significantly improve the resistance of tobacco and peanuts to Pseudomonas solanacearum, and the disease resistance of AhNHL24 is the strongest. Through transgenic tobacco heterologous overexpression of AhNHL24 and inoculation experiments, it is shown that the peanut and tobacco plants overexpressing AhNHL24 not only have significantly enhanced resistance to Pseudomonas solanacearum, but also have significantly enhanced resistance to Sclerotium rofsii. The application provides an important basis for the cultivation of plant (peanut and tobacco, etc.) broad-spectrum disease-resistant varieties.

Provided is an agent for shortening the growth period of a plant, said agent comprising an active ingredient that is a compound represented by formula (I), a tautomer thereof, or an agriculturally acceptable salt of the compound or the tautomer.　(In formula (I), R1 and R2 each independently represent a hydrogen atom or a C1-4 alkyl group, and R3, R4, and R5 each independently represent a C1-4 alkyl group.)

The invention discloses an application of FGSG09291 protein and a coding gene thereof in improving plant disease resistance, and belongs to the technical field of plant immune induced resistance. According to the invention, a novel FGSG09291 protein with a plant immune activation function is found from fusarium graminearum, and the FGSG09291 protein can promote plant active oxygen outbreak and callose accumulation and provide basic immune response for plants; in addition, high expression of the PR1a gene, the WRKY70 gene and the LOX3 gene can be induced, the PR1a gene and the WRKY70 gene are genes of a salicylic acid pathway, and the increase of salicylic acid can improve the broad-spectrum resistance of plants. Experiments prove that the prevention and control effect of corn on dwarf mosaic disease can be enhanced by externally applying the FGSG09291 protein.

The present invention provides an in-vitro method of reducing the efficiency of transducing malignant cells of the blood system of a subject that are not derived from T cells with lentiviral vector particles without reducing the efficiency of transducing T cells in a sample comprising T cells and said malignant cells. A combination of compositions comprising a first composition and a second composition is also disclosed, wherein said first composition comprises i) transduced T cells of a subject, wherein said transduced T cells express a CAR comprising an antigen binding domain, wherein the antigen binding domain of said CAR binds specifically to a tag of a tagged polypeptide, and ii) non-transduced malignant cells of the blood system of said subject, and wherein said second composition comprises said tagged polypeptide, wherein said tagged polypeptide binds specifically to an antigen expressed on the surface of said malignant cells. Alternatively, the transduced T cells of said first composition may comprise a nucleic acid encoding a CAR and an inducible gene expression system, and said second composition may comprise an induction agent inducing said gene system.

Provided are methods and systems for producing a cell based product for dietary consumption, methods and systems for selecting cell lines with heat shock inducible genes associated with a fat or muscle phenotype, draw and fill methods and systems, methods and systems for a chemostat with cell retention, chicken or bovine cell lines, and edible bovine or avian cell lines obtained from the methods described herein.

A myogenin-expressing fibroblast-like cell (MEFLC) line and a construction method and a use thereof are provided. The MEFLC line was deposited in the Guangdong Microbial Culture Collection Center (GDMCC) on Apr. 23, 2022 with an accession number of GDMCC NO: 62409. The construction method includes: 1) constructing a pCW-MYOG-T2A-Puro lentivirus; 2) infecting a human induced pluripotent stem cell (iPSC) line with the lentivirus, inducing the expression of an MYOG gene, and screening out a positive monoclonal cell line; 3) subjecting the positive monoclonal cell line to expanded cultivation, during which the expression of the MYOG gene is continuously induced; and 4) changing cultivation conditions, continuously inducing the expression of the MYOG gene, and screening out positive monoclonal cell lines to ensure the purity of MYOG-positive cells until a cell morphology changes significantly into fibroblastoid cells to obtain the MEFLC line.

The application provides a LiCOMT gene for regulating synthesis of lycoris kingianus glycosides and application thereof, belongs to the technical field of plant genetic engineering, and the nucleotide sequence of the LiCOMT gene is shown as SEQ ID NO. 7, and the amino acid sequence of the encoded protein is shown as SEQ ID NO. 8; the application screens out a candidate gene LiCOMT significantly related to lycoris kingianus glycoside accumulation from lily bulb development period transcriptome data through weighted gene co-expression network analysis (WGCNA), and confirms that silencing the LiCOMT gene can reduce the content of lycoris kingianus glycoside A, lycoris kingianus glycoside B and lycoris kingianus glycoside F in lily bulbs by about 60%, 42% and 43% respectively by using a virus-induced gene silencing (VIGS) technique; and the application provides important gene resources and theoretical basis for lily secondary metabolism regulation and molecular breeding.

This invention belongs to the field of plant genetic engineering technology, specifically relating to a method for improving the disease resistance of rice. SCRE10 Genes and their applications, the aforementioned SCRE10 The base sequence of the gene is shown in SEQ ID NO.1, and the amino acid sequence is shown in SEQ ID NO.2. This invention utilizes a dexamethasone-induced expression mechanism. SCRE10 The transgenic rice plants were found to significantly induce... PR Gene expression and reactive oxygen species surges, enhancing rice resistance to rice false smut and bacterial blight. This invention demonstrates heterologous induction of rice false smut pathogen expression. SCRE10 Genes have the function of positively regulating the disease resistance of rice. SCRE10 Genes can be used to improve the disease resistance of rice, which is of great significance for the creation of disease-resistant rice germplasm.

The invention provides antisense oligonucleotide for regulating and controlling IL1RL2 gene variable splicing as well as a preparation method and application of the antisense oligonucleotide. Specifically, the invention provides an antisense oligonucleotide which interferes with variable splicing of an IL1RL2 gene, thereby inducing the generation of a truncated or concealed exon insertion type IL1RL2 splicing isomer transcript. The antisense oligonucleotide therapy provided by the invention can reduce the proportion of mRNA for coding full-length functional IL-36R and realize activation and blocking of an IL-36R signal channel by inducing splicing jump of an exon 4 or an exon 10 of an IL1RL2 gene precursor mRNA or splicing insertion of a hidden exon sequence of an intron 8. The compound can be used for treating autoimmune diseases, allergic diseases, neurodegenerative diseases or cancers related to abnormal activation of IL-36R.

The invention discloses a dsRNA molecule targeting a VdIRE1 gene and application of the dsRNA molecule in prevention and treatment of verticillium wilt, and belongs to the technical field of biology. The dsRNA molecule can target a verticillium dahliae VdIRE1 gene (encoding an amino acid sequence as shown in SEQ ID NO: 3) and inhibit expression of the verticillium dahliae VdIRE1 gene, and a positive-sense strand nucleotide sequence of the dsRNA molecule is selected from SEQ ID NO: 4 or SEQ ID NO: 5. Based on the molecule, the invention further provides a recombinant vector containing the coding sequence, a recombinant microorganism, a transgenic disease-resistant plant and an RNAi pesticide preparation. The scheme can be realized through host-induced gene silencing, microorganism-mediated gene silencing or preparation treatment and the like, the virulence of pathogenic bacteria can be effectively reduced, and a new technical approach is provided for green prevention and control of verticillium wilt.

This invention describes a transgenic non-human animal model for testing postnatal conditionally inducible plasmalogen deficiency. The genome of the animal model comprises genes capable of regulating the plasmalogen biosynthesis pathway, and these genes have regulatory regions. Within the regulatory regions are at least one conditionally inducible gene editing site that, when edited, blocks gene expression. The genome also has nucleic acid editing sequences that are integrated into a locus separate from the gene encoding the gene product. The gene product, when conditionally induced, edits the gene editing site, ultimately downregulating or disrupting the plasmalogen biosynthesis pathway. Methods and uses comprising the transgenic non-human animal model are also provided.

Provided are methods and systems for producing a cell based product for dietary consumption, methods and systems for selecting cell lines with heat shock inducible genes associated with a fat or muscle phenotype, draw and fill methods and systems, methods and systems for a chemostat with cell retention, chicken or bovine cell lines, and edible bovine or avian cell lines obtained from the methods described herein.

The invention discloses a verticillium dahliae virulence gene, a verticillium dahliae virulence protein and application. The invention relates to the technical field of biology, and provides a method for preventing and treating cotton verticillium wilt based on RNAi (RNA interference) as well as related components and application thereof. Specifically, the invention discloses application of preventing and treating verticillium wilt by a method for inhibiting or silencing the expression of verticillium dahliae pathogenic gene VdHAT1 (the encoding protein of the verticillium dahliae pathogenic gene VdHAT1 is as shown in SEQ ID NO: 3), the core of the verticillium dahliae pathogenic gene VdHAT1 is to provide dsRNA molecules capable of targeting the gene, and the positive-sense strand of the dsRNA molecules is preferably selected from SEQ ID NO: 4, SEQ ID NO: 6 or SEQ ID NO: 7. Based on the molecule, the invention further provides a recombinant vector containing the coding sequence, a recombinant microorganism, a transgenic disease-resistant plant and an RNAi pesticide preparation. The scheme can be realized through host-induced gene silencing, microorganism-mediated gene silencing or preparation treatment and the like, the virulence of pathogenic bacteria can be effectively reduced, and a new technical approach is provided for green prevention and control of verticillium wilt.

The application discloses a dsRNA molecule targeting VdIRE1 a gene and application thereof in prevention and treatment of verticillium wilt, and belongs to the technical field of biotechnology. VdIRE1 The application specifically discloses a dsRNA molecule which can target a Verticillium dahliae gene (coding the amino acid sequence shown in SEQ ID NO: 3) and inhibit expression of the gene, and a nucleotide sequence of a sense strand of the dsRNA molecule is selected from SEQ ID NO: 4 or SEQ ID NO: 5. Based on the molecule, the application further provides a recombinant carrier containing a coding sequence of the molecule, a recombinant microorganism, a transgenic disease-resistant plant and an RNAi pesticide preparation. The schemes can be realized by means of host-induced gene silencing, microorganism-mediated gene silencing or preparation treatment, and can effectively reduce virulence of a pathogenic bacterium, thereby providing a new technical approach for green prevention and control of verticillium wilt.

Provided is an antisense oligonucleotide which interferes with the alternative splicing of an IL1RL2 gene, thereby inducing the production of a truncated IL1RL2 splice isoform transcript. The antisense oligonucleotide can reduce the proportion of mRNA encoding full-length functional IL-36R by means of inducing exon 4 skipping in the pre-mRNA of the IL1RL2 gene, thereby blocking the activation of an IL-36R signaling pathway, and can be used for treating autoimmune diseases, allergic diseases, neurodegenerative diseases or cancers associated with abnormal activation of IL-36R.

The present application belongs to the field of agricultural plant pathology and plant protection technology, and relates to a Plasmodiophora brassicae Pb035 gene and application thereof in prevention and control of Plasmodiophora brassicae. The core of the present application is to use host-induced gene silencing (HIGS) technology or exogenous RNA interference fragment delivery technology to silence Plasmodiophora brassicae Pb035 gene expression, thereby enhancing the resistance of Brassicaceae crops to Plasmodiophora brassicae. The present application provides a key technology and method for prevention and control of Plasmodiophora brassicae, and has broad agricultural application prospect and market value.

This invention relates to the Bg.MEW_38304 gene regulating plant growth and flowering, its encoded protein, and its applications, belonging to the fields of plant molecular biotechnology and genetic engineering. This gene is derived from *Prunus glabra*, with a full-length CDS sequence of 378 bp. The encoded protein contains a conserved SBP domain, and its nucleotide sequence is shown in SEQ ID NO.1. It was cloned from the bracts of the 'Snow White' variety at the BR3 stage. Bioinformatics analysis, subcellular localization, virus-induced gene silencing, and heterologous overexpression experiments verified that the protein encoded by this gene is located in the cell nucleus and cell membrane. Silencing this gene leads to bract and stem / leaf abortion in *Prunus glabra*, while overexpression promotes growth and earlier flowering in *Arabidopsis thaliana*. This application identifies a key gene for *Prunus glabra* bract development, which can be used to promote plant growth and flowering, and has important application value in the molecular breeding of ornamental plants.

The disclosure relates to pharmaceutical compositions and methods of using pharmaceutical compositions comprising effective dosages of an adenoviral-based biological delivery and expression system for use in the treatment or prevention of osteoarthritis in human or mammalian joints by long-term inducible gene expression of human or mammalian interleukin-1 receptor antagonist (IL-1Ra) in synovial cells, comprising a helper-dependent adenoviral vector containing a nucleic acid sequence encoding for human or mammalian interleukin-1 receptor antagonist (IL-1Ra), left and right inverted terminal repeats (L ITR and R ITR), the adenoviral packaging signal and non-viral, non-coding stuffer nucleic acid sequences, wherein the expression of the human or mammalian interleukin-1 receptor antagonist (IL-1Ra) gene within synovial cells is regulated by an inflammation-sensitive promoter.

The process of inducing pre-mRNA splicing was disclosed. CD33 Novel antisense oligonucleotides that skip exon 2 in genes and their potential applications in treating neurodegenerative diseases such as Alzheimer's disease.

The invention belongs to the technical field of molecular plant pathology, and particularly relates to a peanut disease-resistant gene AhNHL24 and application thereof. Through whole genome identification and bioinformatics analysis, 45 NHL family genes (AhNHL) are totally identified in peanuts, and gene expression analysis shows that most AhNHL genes can respond to induction of biological stress and abiotic stress. The gene disease resistance identification finds that the instantaneous overexpression of AhNHL14, AhNHL24 and AhNHL31 can significantly improve the resistance of tobacco and peanut to ralstonia solanacearum, and the disease resistance of AhNHL24 is the strongest. The transgenic tobacco heterologous overexpression AhNHL24 and inoculation experiments show that not only is the resistance of peanut and tobacco plants for overexpression AhNHL24 obviously enhanced to ralstonia solanacearum, but also the resistance to sclerotium rolfsii is also obviously enhanced. The invention provides an important basis for cultivation of broad-spectrum disease-resistant varieties of plants (peanuts, tobaccos and the like).

The application provides application of MdPHY7 protein or MdPHY7 gene related to apple anthocyanin biosynthesis in regulation of apple anthocyanin synthesis, and belongs to the technical field of plant genetic engineering. Exogenous ALA treatment can induce MdPHY7 gene expression, thereby promoting anthocyanin synthesis increase, finally causing apple anthocyanin accumulation and promoting fruit coloring. It has been verified that overexpression of MdPHY7 gene can promote apple anthocyanin accumulation, while interference with MdPHY7 gene expression can inhibit anthocyanin accumulation and further inhibit fruit appearance quality improvement. It can be seen that MdPHY7 is a new key gene for regulating apple anthocyanin accumulation, and plays a key role in ALA regulation of apple anthocyanin accumulation, which has far-reaching significance for agricultural and forestry production, and lays a solid theoretical foundation for application of ALA in the field of agricultural production.

The disclosure provides for ribozyme-mediated fusion constructs and systems and methods thereof, for use in a variety of applications, including for inducible gene expression systems, gene therapy, and combinatorial screening.