117 results about "Inducible gene" patented technology

The invention belongs to the technical field of plant genetic engineering, and particularly relates to an SCRE10 gene for improving rice disease resistance and application of the SCRE10 gene, the base sequence of the SCRE10 gene is shown as SEQ ID NO.1, and the amino acid sequence of the SCRE10 gene is shown as SEQ ID NO.2. Through construction of a dexamethasone induced expression SCRE10 transgenic rice plant, it is found that the transgenic rice can significantly induce PR gene expression and active oxygen outbreak, and the resistance of rice to false smut and bacterial leaf blight can be improved. The invention proves that heterologous inducible expression of the Ustilaginoidea virens SCRE10 gene has the function of positively regulating the disease resistance of the rice, and the SCRE10 gene can be used for improving the disease resistance of the rice, which is of great significance to the creation of disease-resistant germplasm of the rice.

The present invention provides a self-activating small molecule induction system, cells, and applications and methods in inducing gene expression, belonging to the technical field of genetic engineering. The self-activating small molecule induction system optimizes the component composition based on the green light response system, improves the induction efficiency of the target gene through a cascade reaction, and can reduce the background leakage level, playing an important role in the induction of the target gene and having good application prospects in the field of protein expression and the production and preparation of viral vectors.

The invention relates to a mutant gene of a rice OsSPL10 gene and application of the mutant gene. The nucleotide sequence of the mutant gene is shown as SEQ IN NO.1, and the mutant gene can endow rice with resistance to glufosinate-ammonium herbicide; according to the invention, a rice variety 'Yangxian 9B' is treated by an EMS chemical mutagenesis technology, mutation of the OsSPL10 gene is successfully induced, and the mutation causes that the 225th amino acid in the amino acid sequence of the encoded protein is changed into tyrosine from cysteine; the application also develops a KASP molecular marker for the variation site, molecular marker detection and field glufosinate-ammonium resistance screening and verification prove that rice with the mutant gene can improve the glufosinate-ammonium resistance, and the gene has significant application potential in agricultural production, and can reduce the use of herbicides and improve the crop yield and quality.

The present disclosure relates to the fields of molecular biology and nucleic acid technology. The present disclosure also relates to therapy and prophylaxis of disease.

The invention discloses a verticillium dahliae virulence gene, a verticillium dahliae virulence protein and application. The invention relates to the technical field of biology, and provides a method for preventing and treating cotton verticillium wilt based on RNAi (RNA interference) as well as related components and application thereof. Specifically, the invention discloses application of the verticillium wilt prevention and treatment by a method for inhibiting or silencing the expression of verticillium dahliae pathogenic gene VdHDAC (coding protein of the verticillium dahliae pathogenic gene VdHDAC is shown as SEQ ID NO: 3), the core of the verticillium wihliae pathogenic gene VdHDAC is to provide dsRNA molecules capable of targeting the gene, and the positive-sense strand of the dsRNA molecules is preferably selected from SEQ ID NO: 5, SEQ ID NO: 8, SEQ ID NO: 9 or SEQ ID NO: 11. Based on the molecule, the invention further provides a recombinant vector containing the coding sequence, a recombinant microorganism, a transgenic disease-resistant plant and an RNAi pesticide preparation. The scheme can be realized through host-induced gene silencing, microorganism-mediated gene silencing or preparation treatment and the like, the virulence of pathogenic bacteria can be effectively reduced, and a new technical approach is provided for green prevention and control of verticillium wilt.

Provided is a recombinant promoter system specifically inducing gene expression after T cell activation. Also provided is a new T cell activation-induced recombinant promoter constructed by modifying and optimizing GM-CSF, IFNg and IL2 promoters. When CAR-T cells are activated, the recombinant promoter can efficiently induce the expression of downstream genes, with the activity thereof being superior to that of a traditional NFAT-responsive promoter constructed based on a binding sequence of a nuclear factor of activated T cells. Further provided are a CAR-T cell comprising the T cell activation-induced recombinant promoter and the use thereof.

The invention relates to the technical field of plant genetic engineering, and particularly provides cloning of a GbGELP25D gene of gossypium barbadense and application of the GbGELP25D gene of gossypium barbadense. According to the invention, the GbGELP25D gene with a full length of 1092 bp is cloned from the sea island cotton variety Xinhai No. 7 for the first time, and the gene encodes a secretory lipase protein with a signal peptide and is positioned in an extracellular gap. Through bioinformatics analysis, phylogenetic classification, protein structure modeling and signal peptide function verification, it is clear that the gene belongs to a plant GELP family. Furthermore, a virus-induced gene silencing technology is utilized to prove that the silent GbGELP25D can obviously enhance the resistance of cotton to verticillium wilt, and the mechanism of the silent GbGELP25D is closely related to activation of ethylene synthesis and signal channels and induction of expression of disease-resistant related genes. The resistance gene provided by the invention enriches gene resources of cotton verticillium wilt resistance breeding, and has important theoretical significance and application value.

The invention belongs to the technical field of agricultural plant pathology and plant protection, and relates to a plasmodiophora brassicae Pb035 gene and application thereof in prevention and control of clubroot of cruciferae crops. The core of the invention lies in that the plasmodiophora brassicae Pb035 gene expression is silenced by using a host induced gene silencing (HIGS) technology or an exogenous RNA interference fragment delivery technology, so that the resistance of cruciferae crops to clubroot is enhanced. The discovery provides a key technology and method for prevention and control of clubroot of cruciferae crops, and has wide agricultural application prospect and market value.

The invention discloses a nucleic acid construct for inducing DDB2 gene silencing and application of the nucleic acid construct. The designed targeted expression system based on the adeno-associated virus (AAV) vector is higher in specificity, non-target tissue can be prevented from being interfered, the tissue specificity of gene intervention is improved, and the off-target risk is small; the shRNA has high stability and processing efficiency, reduces immune response and interference effect, has high expression efficiency and strong histocompatibility, and is suitable for long-term expression intervention in vivo.

In one aspect, a method of treating an NK cell to induce a gene edit. The method may include contacting an NK cell with a transfection composition including poly-L-glutamic acid (y- PGA), a single-stranded oligo deoxynucleotide (ssODN) enhancer, or a combination thereof; contacting the NK cell with an editing composition including: a DNA-PK inhibitor, and an HD AC inhibitor; electroporating the NK cell; and contacting the NK cell with a recovery composition including DNase I. In another aspect, a method of preparing an engineered NK cell comprising a fluorescent reporter at an endogenous locus. In another aspect, a method of treating an NK cell to introduce an exogenous nucleic acid.

The invention relates to the technical field of biomedicine, in particular to a novel target spot for resisting chikungunya virus infection and application. According to the invention, human colon cancer cells (HCT116) are taken as target cells, and retinoic acid-induced protein expression of the target cells is reduced by adopting a gene knockout technology, so that host factors capable of effectively inhibiting CHIKV infection of the human colon cancer cells are found, and the purpose of blocking CHIKV infection from the source is achieved. It is found that retinoic acid induced protein 16 (RAI16) plays an important role in CHIKV infected HCT116 cells, and CHIKV infection can be obviously promoted by down-regulating expression of RAI16. The invention provides an application of RAI16 in preparation of a medicine for preventing or treating chikungunya virus infection, and provides a new target spot and a treatment scheme for clinically preventing and treating fever, arthralgia, arthrocele, muscular pain, headache, nausea, fatigue, rash and other diseases caused by CHIKV infection.

The application belongs to the technical field of molecular plant pathology, and particularly relates to a peanut disease-resistant gene AhNHL24 and application thereof. Through whole genome identification and bioinformatics analysis, 45 NHL family genes (AhNHL) are identified in peanuts, and gene expression analysis shows that most of the AhNHL genes can respond to the induction of biological stress and abiotic stress. Gene disease resistance identification finds that transient overexpression of AhNHL14, AhNHL24 and AhNHL31 can significantly improve the resistance of tobacco and peanuts to Pseudomonas solanacearum, and the disease resistance of AhNHL24 is the strongest. Through transgenic tobacco heterologous overexpression of AhNHL24 and inoculation experiments, it is shown that the peanut and tobacco plants overexpressing AhNHL24 not only have significantly enhanced resistance to Pseudomonas solanacearum, but also have significantly enhanced resistance to Sclerotium rofsii. The application provides an important basis for the cultivation of plant (peanut and tobacco, etc.) broad-spectrum disease-resistant varieties.

The instant disclosure provides chimeric polypeptides which modulate various cellular processes following a cleavage event induced upon binding of a specific binding member of the polypeptide with its binding partner. Methods of using chimeric polypeptides to modulate cellular functions, including e.g., induction of gene expression, are also provided. Nucleic acids encoding the subject chimeric polypeptides and associated expression cassettes and vectors as well as cells that contain such nucleic acids and / or expression cassettes and vectors are provided. Also provided, are methods of treating a subject using the described components and methods as well as kits for practicing the subject methods.

The invention discloses a light-induced gene promoter, a recombinant plasmid, a recombinant bacterium and an application of the light-induced gene promoter. The nucleotide sequence of the light-induced gene promoter is as shown in SEQ ID No: 1. The recombinant plasmid comprises an expression vector and the light-induced gene promoter. According to the invention, a light-induced gene promoter is used for replacing a constitutive gene promoter, and two recombinant vectors, namely, pbHLH93-LUC and pbHLH93-GUS, containing specific light-induced promoters are provided. The two constructed recombinant vectors have the advantages of being good in specificity, efficient in expression, easy to screen and the like, and the recombinant vector pbHLH93-GUS can completely rapidly obtain a transgenic material for photoinduced expression of a target gene on the basis of ensuring illumination, and is expected to play an important role in regulation and control of expression of the target gene.

The invention provides a self-activated micromolecule induction system, a cell and application and a method in inducing gene expression, and belongs to the technical field of gene engineering. According to the self-activated small molecule induction system, element composition optimization is carried out on the basis of a green light response system, the induction efficiency of a target gene is improved through cascade reaction, the background leakage level can be reduced, the self-activated small molecule induction system can play an important role in target gene induction, and the application prospect is wide. The method has a good application prospect in the field of protein expression and virus vector production and preparation.

Disclosed herein are optogenetic circuits for the bacterium Escherichia coli that induce gene expression in darkness and repress it under blue light. Applying them to metabolic engineering improves chemical production compared to chemically induced controls in light-controlled fermentations. More particularly, these circuits can be used to control protein production with light. The system and method use light as a suitable alternative to chemical induction for microbial production of chemicals and proteins.

The present disclosure, at least in part, relates to compositions (e.g., engineered nucleic acids and engineered proteins) and methods for increasing gene expression. The engineered proteins include RNA-binding proteins (e.g., RNA-binding proteins that comprise a Interleukin Enhancer Binding Factor 3 (ILF3) sequence, a Cas sequence, or a combination thereof). In some aspects, the disclosure provides methods of identifying engineered nucleic acids that are shorter in length than a gene of interest to induce expression of the gene of interest and also provides RNA-binding proteins for inducing gene expression.

The present invention discloses a construction method and application of a mouse conditional inducible Hmox1 gene knockout model, and belongs to the field of biotechnology. The mouse conditional inducible Hmox1 gene knockout model provided by the present invention is constructed by the following steps: constructing a homologous recombination DNA vector; obtaining Cas9mRNA and gRNA for the Hmox1 gene by in vitro transcription; injecting the homologous recombination DNA vector, Cas9mRNA and gRNA into the fertilized egg of the mouse; cultivating to obtain conditional gene knockout mice; identifying the genotype by sequencing and nucleic acid gel electrophoresis; the obtained conditional knockout mice are hybridized with other tissue-specific Cre-expressing mice to obtain corresponding tissue or cell conditional knockout mice, thereby realizing precise research on specific tissues or cell types; and proving that Hmox1 inducible conditional knockout significantly aggravates acute lung injury. The present invention can overcome the difficulties of embryonic lethality of Hmox1 systemic gene knockout mice in the prior art and the inability to construct a mouse model, and assist in the study of Hmox1 in diseases.

Provided is an agent for shortening the growth period of a plant, said agent comprising an active ingredient that is a compound represented by formula (I), a tautomer thereof, or an agriculturally acceptable salt of the compound or the tautomer.　(In formula (I), R1 and R2 each independently represent a hydrogen atom or a C1-4 alkyl group, and R3, R4, and R5 each independently represent a C1-4 alkyl group.)

The invention discloses an application of FGSG09291 protein and a coding gene thereof in improving plant disease resistance, and belongs to the technical field of plant immune induced resistance. According to the invention, a novel FGSG09291 protein with a plant immune activation function is found from fusarium graminearum, and the FGSG09291 protein can promote plant active oxygen outbreak and callose accumulation and provide basic immune response for plants; in addition, high expression of the PR1a gene, the WRKY70 gene and the LOX3 gene can be induced, the PR1a gene and the WRKY70 gene are genes of a salicylic acid pathway, and the increase of salicylic acid can improve the broad-spectrum resistance of plants. Experiments prove that the prevention and control effect of corn on dwarf mosaic disease can be enhanced by externally applying the FGSG09291 protein.

The present invention provides an in-vitro method of reducing the efficiency of transducing malignant cells of the blood system of a subject that are not derived from T cells with lentiviral vector particles without reducing the efficiency of transducing T cells in a sample comprising T cells and said malignant cells. A combination of compositions comprising a first composition and a second composition is also disclosed, wherein said first composition comprises i) transduced T cells of a subject, wherein said transduced T cells express a CAR comprising an antigen binding domain, wherein the antigen binding domain of said CAR binds specifically to a tag of a tagged polypeptide, and ii) non-transduced malignant cells of the blood system of said subject, and wherein said second composition comprises said tagged polypeptide, wherein said tagged polypeptide binds specifically to an antigen expressed on the surface of said malignant cells. Alternatively, the transduced T cells of said first composition may comprise a nucleic acid encoding a CAR and an inducible gene expression system, and said second composition may comprise an induction agent inducing said gene system.

The present disclosure provides compositions and methods for modifying an ANGPTL3 gene. In some aspects, the present disclosure provides a guide RNA, compositions thereof, and pharmaceutical compositions comprising a guide RNA or a composition as described herein. In some aspects, the present disclosure also provides uses and methods of using a guide RNA, a composition thereof, or a pharmaceutical composition as described herein, for inducing a double-stranded break in an ANGPTL3 gene, for reducing expression of an ANGPTL3 gene in a cell or subject, and for treating a patient having or at risk of having an ANGPTL3 -related disease or condition.

Compositions and methods of use thereof for delivering nucleic acid cargo into cells are provided. The compositions typically include (a) a 3E10 monoclonal antibody or an antigen binding, cell-penetrating fragment thereof; a monovalent, divalent, or multivalent single chain variable fragment (scFv); or a diabody; or humanized form or variant thereof, and (b) a nucleic acid cargo including, for example, a nucleic acid encoding a polypeptide, a functional nucleic acid, a nucleic acid encoding a functional nucleic acid, or a combination thereof. Elements (a) and (b) are typically non-covalently linked to form a complex.

Provided are methods and systems for producing a cell based product for dietary consumption, methods and systems for selecting cell lines with heat shock inducible genes associated with a fat or muscle phenotype, draw and fill methods and systems, methods and systems for a chemostat with cell retention, chicken or bovine cell lines, and edible bovine or avian cell lines obtained from the methods described herein.

The invention discloses a novel butenolide signal molecule and application thereof in gene expression regulation and control. The invention provides a novel butenolide signal molecule SFB1. The structure of the novel butenolide signal molecule SFB1 is shown as a formula I). An inducible gene expression regulation and control circuit composed of the SFB1, the receptor protein of the SFB1 and the heterozygous promoter can efficiently work in escherichia coli, that is, the signal molecule SFB1 with nanomolar concentration can effectively remove repression of the receptor protein TylP on the heterozygous promoter and induce expression of a target gene; other types of streptomycete signal molecules including butenolide and gamma-butyric acid lactone from other streptomycetes cannot remarkably remove repression of TylP on the hybrid promoter, that is, the regulation and control circuit disclosed by the invention is not interfered by other types of common signal molecules in the streptomycetes, and can be used in combination with the existing AHL or GBL regulation and control tools; and a synthetic biological tool library is enriched.

A myogenin-expressing fibroblast-like cell (MEFLC) line and a construction method and a use thereof are provided. The MEFLC line was deposited in the Guangdong Microbial Culture Collection Center (GDMCC) on Apr. 23, 2022 with an accession number of GDMCC NO: 62409. The construction method includes: 1) constructing a pCW-MYOG-T2A-Puro lentivirus; 2) infecting a human induced pluripotent stem cell (iPSC) line with the lentivirus, inducing the expression of an MYOG gene, and screening out a positive monoclonal cell line; 3) subjecting the positive monoclonal cell line to expanded cultivation, during which the expression of the MYOG gene is continuously induced; and 4) changing cultivation conditions, continuously inducing the expression of the MYOG gene, and screening out positive monoclonal cell lines to ensure the purity of MYOG-positive cells until a cell morphology changes significantly into fibroblastoid cells to obtain the MEFLC line.

The application provides a LiCOMT gene for regulating synthesis of lycoris kingianus glycosides and application thereof, belongs to the technical field of plant genetic engineering, and the nucleotide sequence of the LiCOMT gene is shown as SEQ ID NO. 7, and the amino acid sequence of the encoded protein is shown as SEQ ID NO. 8; the application screens out a candidate gene LiCOMT significantly related to lycoris kingianus glycoside accumulation from lily bulb development period transcriptome data through weighted gene co-expression network analysis (WGCNA), and confirms that silencing the LiCOMT gene can reduce the content of lycoris kingianus glycoside A, lycoris kingianus glycoside B and lycoris kingianus glycoside F in lily bulbs by about 60%, 42% and 43% respectively by using a virus-induced gene silencing (VIGS) technique; and the application provides important gene resources and theoretical basis for lily secondary metabolism regulation and molecular breeding.

This invention belongs to the field of plant genetic engineering technology, specifically relating to a method for improving the disease resistance of rice. SCRE10 Genes and their applications, the aforementioned SCRE10 The base sequence of the gene is shown in SEQ ID NO.1, and the amino acid sequence is shown in SEQ ID NO.2. This invention utilizes a dexamethasone-induced expression mechanism. SCRE10 The transgenic rice plants were found to significantly induce... PR Gene expression and reactive oxygen species surges, enhancing rice resistance to rice false smut and bacterial blight. This invention demonstrates heterologous induction of rice false smut pathogen expression. SCRE10 Genes have the function of positively regulating the disease resistance of rice. SCRE10 Genes can be used to improve the disease resistance of rice, which is of great significance for the creation of disease-resistant rice germplasm.

The invention provides antisense oligonucleotide for regulating and controlling IL1RL2 gene variable splicing as well as a preparation method and application of the antisense oligonucleotide. Specifically, the invention provides an antisense oligonucleotide which interferes with variable splicing of an IL1RL2 gene, thereby inducing the generation of a truncated or concealed exon insertion type IL1RL2 splicing isomer transcript. The antisense oligonucleotide therapy provided by the invention can reduce the proportion of mRNA for coding full-length functional IL-36R and realize activation and blocking of an IL-36R signal channel by inducing splicing jump of an exon 4 or an exon 10 of an IL1RL2 gene precursor mRNA or splicing insertion of a hidden exon sequence of an intron 8. The compound can be used for treating autoimmune diseases, allergic diseases, neurodegenerative diseases or cancers related to abnormal activation of IL-36R.