151 results about "Verticillium wilt" patented technology

The invention relates to the technical field of biology, in particular to a verticillium dahliae virulence gene, a verticillium dahliae virulence protein and application. The invention discloses a verticillium dahliae virulence gene VdPHO23like. The verticillium dahliae virulence gene VdPHO23like comprises a polynucleotide sequence for coding an amino acid sequence of SEQ ID NO: 3. According to the invention, expression of the gene is inhibited in a targeted manner through an RNA interference mediated gene silencing technology so as to weaken virulence of pathogenic bacteria, and an efficient, specific and environment-friendly comprehensive prevention and control strategy is provided for verticillium wilt of crops such as cotton.

The invention discloses an upland cotton GhCRK29 gene as well as an encoding protein and application thereof, the GhCRK29 gene is separated from cotton, the nucleotide sequence of the GhCRK29 gene is as shown in SEQ ID No.1, and the amino acid sequence of the encoding protein of the GhCRK29 gene is as shown in SEQ ID No.2. According to the invention, a virus-induced gene silencing technology and a transgenic overexpression technology are further utilized to carry out preliminary research on the function of the cotton GhCRK29 gene in cotton verticillium wilt resistance, and a result shows that the verticillium wilt resistance of a cotton seedling plant is obviously reduced after the GhCRK29 gene in cotton is silenced; after the GhCRK29 gene is over-expressed in arabidopsis thaliana or cotton, the verticillium wilt resistance of arabidopsis thaliana or cotton is remarkably improved, and the resistance of a GhCRK29 gene forward regulation plant to verticillium dahliae is determined; the invention has application prospects in the aspects of improving the verticillium wilt resistance of plants or cultivating verticillium wilt resistant plant varieties and the like.

The invention discloses bacillus velezensis BR-1 and application of the bacillus velezensis BR-1 in prevention and treatment of economic crop diseases. The preservation number of the bacillus velezensis BR-1 is CCTCC NO: M 20251379. The bacillus velezensis BR-1 disclosed by the invention is high in adaptability and wide in growth condition range, and is more beneficial to application in agricultural production. Meanwhile, the bacillus velezensis BR-1 has a remarkable inhibition effect on the growth of various phytopathogens such as verticillium dahliae, fusarium oxysporum, sclerotinia sclerotiorum and ralstonia solanacearum, can remarkably reduce the morbidity of cotton verticillium wilt, fusarium wilt and tomato bacterial wilt under a greenhouse condition, and has a wide agricultural application prospect.

The invention relates to the technical field of microorganisms, and particularly provides application of bacillus amyloliquefaciens BA-89 in crop growth promotion and verticillium dahliae antagonism. The strain is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.36498. The strain can secrete multiple extracellular enzymes (including protease, amylase, cellulase and glucanase), has the growth promoting characteristics of producing ammonia, dissolving potassium and the like, and can tolerate high temperature and ultraviolet stress; the compound has a remarkable antagonistic effect on various plant pathogenic bacteria (such as verticillium dahliae, fusarium oxysporum and phytophthora capsici); by inoculating the strain on cotton, the plant growth can be obviously promoted, the plant height, stem diameter, root length, leaf area and biomass are improved, and meanwhile, the disease index of verticillium wilt and the colonization amount of pathogenic bacteria at the root are effectively reduced. The strain disclosed by the invention can be used as a biological fertilizer and a biological pesticide, is used for cotton cultivation and disease prevention and control, and has a wide application prospect.

The invention belongs to the technical field of agricultural microbial control, and particularly relates to a growth-promoting and disease-preventing compound microbial agent and a preparation method and application thereof, and the compound microbial agent specifically comprises microbial powder, a growth auxiliary material and a drying agent; the microorganisms comprise vermiform yellow, trichoderma harzianum, bacillus velezensis, bacillus licheniformis and brevibacillus laterosporus. According to the compound microbial agent, through the synergistic effect of multiple strains, the control effect of the compound microbial agent on verticillium dahliae is enhanced, and the defects that a single biocontrol bacterium is single in biocontrol mechanism, poor in environment adaptability, incapable of being colonized in soil for a long time and unstable in control effect are overcome; the compound microbial agent can increase the yield of cotton and pepper and improve the quality of cotton fibers, and provides scientific and technological support and decision service for realizing green, environment-friendly, cost-saving and efficiency-increasing modern agriculture.

The invention provides a composite flora for preventing and treating cotton verticillium wilt and a microbial agent and application thereof, and belongs to the technical field of agricultural microorganisms. The composite flora and the microbial agent provided by the invention comprise pseudoarthrobacter oxydans CRB-17 and bacillus velezensis CRB-46; the preservation numbers of the pseudoarthrobacter oxydans CRB-17 and the bacillus velezensis CRB-46 are respectively CCTCC (China Center for Type Culture Collection) NO: M20252254 and CCTCC NO: M20252255, and the pseudoarthrobacter oxydans CRB-17 and the bacillus velezensis CRB-46 are both preserved in the China Center for Type Culture Collection on October 20, 2025. The pseudoarthrobacter oxydans CRB-17 and the bacillus velezensis CRB-46 are stably colonized in the rhizosphere of the cotton, have a strong direct antagonism effect on verticillium dahliae, can effectively and continuously prevent and treat the verticillium wilt of the cotton, effectively promote the germination of cotton seeds, the elongation of radicles and hypocotyls, promote the growth of the cotton and improve the defensive power of the cotton.

The invention discloses a verticillium dahliae virulence gene, a verticillium dahliae virulence protein and application. The invention relates to the technical field of biology, and provides a method for preventing and treating cotton verticillium wilt based on RNAi (RNA interference) as well as related components and application thereof. Specifically, the invention discloses application of the verticillium wilt prevention and treatment by a method for inhibiting or silencing the expression of verticillium dahliae pathogenic gene VdHDAC (coding protein of the verticillium dahliae pathogenic gene VdHDAC is shown as SEQ ID NO: 3), the core of the verticillium wihliae pathogenic gene VdHDAC is to provide dsRNA molecules capable of targeting the gene, and the positive-sense strand of the dsRNA molecules is preferably selected from SEQ ID NO: 5, SEQ ID NO: 8, SEQ ID NO: 9 or SEQ ID NO: 11. Based on the molecule, the invention further provides a recombinant vector containing the coding sequence, a recombinant microorganism, a transgenic disease-resistant plant and an RNAi pesticide preparation. The scheme can be realized through host-induced gene silencing, microorganism-mediated gene silencing or preparation treatment and the like, the virulence of pathogenic bacteria can be effectively reduced, and a new technical approach is provided for green prevention and control of verticillium wilt.

The invention discloses a TLP1-like gene molecular marker related to cotton verticillium wilt resistance and application of the TLP1-like gene molecular marker, and belongs to the field of biotechnology application. The promoter of the TLP1 protein gene TLP1-like has two haplotypes, the promoter sequence of the haplotype 1 is SEQ ID NO.5, and the genomic sequence of the haplotype 2 is SEQ ID NO.6. The invention also discloses a method for preparing the TLP1 protein gene TLP1-like promoter of the TLP1 protein gene TLP1-like promoter of the TLP1 protein gene. Compared with the haplotype 1, the haplotype 2 has an SV insertion of 2623 bp at a-899 bp position of an ATG upstream promoter region of a TLP1-like gene, so that the expression quantity of the TLP1-like gene is reduced. An InDel functional marker capable of remarkably distinguishing the haplotype 1 and the haplotype 2 is further developed. Compared with the haplotype 1, the haplotype 2 has a remarkable advantage in the aspect of improving the verticillium wilt resistance of the cotton, and the silent haplotype 1 cotton plant TLP1-like remarkably improves the resistance of the cotton to verticillium dahliae. The haplotype 2 has important effects and application prospects in screening cotton disease-resistant materials and cultivating cotton high-resistance varieties.

The invention relates to a verticillium wilt resistance gene Gbar4CL1 of cotton and application of the verticillium wilt resistance gene Gbar4CL1. The gene of a cotton verticillium wilt resistance material M34 is subjected to map-based cloning, the cotton verticillium wilt resistance gene Gbar4CL1 is identified for the first time, the Gbar4CL1 gene is proved to be highly expressed after being induced by verticillium wilt, and further the gene is over-expressed in cotton to verify that the gene can obviously enhance the verticillium wilt resistance of the cotton, so that the cotton verticillium wilt resistance of the cotton can be obviously enhanced. The gene has important value for theoretical researches such as analysis of molecular mechanisms of cotton verticillium wilt resistance and the like. The Gbar4CL1 gene can improve the resistance of cotton to verticillium wilt, and has important practical application significance for cotton verticillium wilt resistant breeding.

The invention belongs to the technical field of microorganisms, and discloses bacillus velezensis and application thereof in plant disease control. A bacillus velezensis strain is separated and screened from rhizosphere soil of a wolfberry plant in a Nomuliflood farm in Qinghai province, the strain is bacillus velezensis G3 and is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No. 36136. The bacterial strain has a better antibacterial effect on pathogenic bacteria fusarium oxysporum of Chinese wolfberry root rot, also has an inhibiting effect on pathogenic bacteria of Chinese wolfberry verticillium wilt, botrytis cinerea, sclerotinia rot of colza, alternaria alternata, apple anthracnose, apple rot, pear wilt and wheat scab, and has broad-spectrum antibacterial activity; wide application prospects are realized in plant disease prevention and treatment.

The invention relates to the technical field of agricultural biology, in particular to a verticillium dahliae heat shock protein 90 ATP enzyme activating factor VdAHA1 as well as a coding gene and application of the verticillium dahliae heat shock protein 90 ATP enzyme activating factor VdAHA1. The invention provides a verticillium dahliae heat shock protein 90 ATP enzyme activating factor VdAHA1 related to high temperature resistance, drug resistance, environmental adaptability, ergosterol synthesis capability and pathogenicity of verticillium dahliae and a coding gene of the VdAHA1, and the VdAHA1 gene positively regulates the pathogenicity of verticillium dahliae and can be used as an important candidate gene for pathogenesis research of verticillium dahliae. The invention provides a theoretical basis for further understanding the pathogenic mechanism of pathogenic bacteria, also provides a new gene resource for verticillium dahliae pathogenic related genes, also provides a new idea for prevention and control of cotton verticillium wilt, and has a wide application prospect.

The invention discloses an application of a GhMKK5 gene in improving the disease resistance of cotton verticillium wilt, and belongs to the field of biotechnology application. The GhMKK5 gene disclosed by the invention is used for coding a mitogen activated protein kinase kinase. The invention provides a full-length CDS nucleotide sequence and an amino acid sequence of GhMKK5 in an upland cotton genetic standard line TM-1. The gene is expressed in all tissues of cotton, and is induced by verticillium dahliae to realize obvious up-regulation expression. The verticillium wilt resistance of cotton is reduced by silencing the gene in cotton, and the disease resistance of arabidopsis thaliana is improved by overexpressing the gene in arabidopsis thaliana, which indicates that the gene plays an important role in cotton verticillium wilt resistance.

The invention relates to the technical field of plant genetic engineering, and particularly provides cloning of a GbGELP25D gene of gossypium barbadense and application of the GbGELP25D gene of gossypium barbadense. According to the invention, the GbGELP25D gene with a full length of 1092 bp is cloned from the sea island cotton variety Xinhai No. 7 for the first time, and the gene encodes a secretory lipase protein with a signal peptide and is positioned in an extracellular gap. Through bioinformatics analysis, phylogenetic classification, protein structure modeling and signal peptide function verification, it is clear that the gene belongs to a plant GELP family. Furthermore, a virus-induced gene silencing technology is utilized to prove that the silent GbGELP25D can obviously enhance the resistance of cotton to verticillium wilt, and the mechanism of the silent GbGELP25D is closely related to activation of ethylene synthesis and signal channels and induction of expression of disease-resistant related genes. The resistance gene provided by the invention enriches gene resources of cotton verticillium wilt resistance breeding, and has important theoretical significance and application value.

The invention provides a rare-earth-based composite pesticide and a preparation method and application thereof, active ingredients of the rare-earth-based composite pesticide comprise a soil material LN and carbendazim according to the mass ratio of (2-3): 1, and the soil material LN is prepared after a lanthanide rare earth material and iodic acid react and crystallize. The rare-earth-based composite pesticide disclosed by the invention has an obvious inhibition effect on pathogenic bacteria of cotton verticillium wilt, can obviously improve symptoms of the cotton verticillium wilt in field experiments and practical application processes, and reduces agricultural loss caused by the cotton verticillium wilt.

The invention belongs to the technical field of biology, and discloses a peptide signal molecule GhEG45 and application of a gene engineering product of the peptide signal molecule GhEG45 in plant growth regulation and disease resistance. The gene coding sequence of the peptide signal molecule GhEG45 is as shown in SEQ ID NO. 1, and the amino acid sequence of the peptide signal molecule GhEG45 is as shown in SEQ ID NO. 2. The peptide signal molecule GhEG45 can prevent and treat cotton verticillium wilt and regulate plant growth, can also be used for cultivating new varieties of transgenic plants resisting V.dahliae infection, improves the disease resistance and stress resistance of the plants, plays a role in regulating plant growth, increases both production and income, and improves crop quality.

This application discloses a Bacillus subtilis strain JTFB-01 and its applications, belonging to the field of microbiology. The Bacillus subtilis strain JTFB-01 has the accession number CGMCC No. 37199 and is deposited at the China General Microbiological Culture Collection Center on December 25, 2025. Field trials show that this inoculant, applied through seed treatment, achieves control effects of 68.20%, 71.73%, and 72.15% against wheat take-all, sheath blight, and root rot, respectively, and significantly increases wheat plant height and yield. It also shows a field control effect of over 50% against cotton Verticillium wilt, with a yield increase of over 17%. This inoculant effectively controls soil-borne diseases and overcomes crop replanting obstacles through multiple mechanisms, including inhibiting pathogens, colonizing the rhizosphere, and promoting growth, thus possessing significant agricultural application value.

The invention provides a rare earth functional material for preventing and treating cotton verticillium wilt and a preparation method and application thereof.The rare earth functional material comprises an intermediate, a carrier and water according to the mass ratio of (4-6): (4-6): (18-25), and the intermediate comprises a rare earth lanthanum source compound, a medium and water according to the mass ratio of (7-15): (2-7): (78-89). The rare earth functional material LCP has a particularly strong inhibition effect on verticillium dahliae which is a pathogenic bacterium of cotton verticillium wilt, compared with a common pesticide thiophanate methyl in the market, the inhibition effect of the rare earth functional material LCP is obviously better than that of the pesticide thiophanate methyl, the toxic effect on plants is low, the material is expected to be applied to preparation of pesticides, and the application prospect is wide. The composition is used for controlling cotton verticillium wilt, increasing cotton yield in China and improving cotton quality.

The invention discloses a cotton verticillium wilt resistance related gene GhACA12 and application thereof in disease-resistant breeding, and belongs to the technical field of agricultural biology. Through whole genome association analysis (GWAS), an SNP site D11: 57218252 related to verticillium wilt resistance is found in a cotton D11 chromosome region, and a GhACA12 gene is screened out as a candidate disease-resistant gene. Researches show that high expression of GhACA12 is related to susceptible characters, and low expression of GhACA12 is related to disease resistance characters. The GhACA12 gene is silenced through a VIGS technology, and the disease resistance of the cotton is remarkably enhanced. Specific primers are designed for qRT-PCR detection, and disease-resistant materials are screened in an auxiliary mode. The invention provides effective technical support for cotton verticillium wilt resistant molecular breeding, and has important application value.

The invention discloses a microbial agent for preventing and treating cotton verticillium wilt and a preparation method and application thereof, the microbial agent comprises a bacterial strain of bacillus velezensis and / or a fermentation broth thereof, and an agriculturally acceptable solid carrier for loading the bacterial strain, and the preservation number of the bacterial strain is CCTCC NO: M 2022954. According to the microbial agent, bacillus velezensis BAC81712 serves as an antagonistic bacterium and is loaded on a solid carrier, and the microbial agent has the advantages of being convenient to use, low in microorganism colonization difficulty, high in bacteriostasis rate, stable in prevention and treatment effect and the like, can be widely used for preventing and treating cotton verticillium wilt in potted plants and fields, can remarkably reduce the morbidity of the cotton verticillium wilt and has a good application prospect. The cotton plant morphology can be obviously improved, the use value is high, and the application prospect is good.

The invention relates to the technical field of microorganisms, and particularly provides application of bacillus velezensis Y1062 in crop growth promotion and verticillium dahliae antagonism. The strain is preserved in the China General Microbiological Culture Collection Center (CGMCC) with the preservation number of CGMCC No.36497, has various probiotic functions of dissolving phosphorus and potassium, secreting cellulase, amylase and protease and the like, and can tolerate high temperature and ultraviolet stress. Meanwhile, the strain has a broad-spectrum antagonistic effect on various phytopathogens such as verticillium dahliae, fusarium oxysporum and the like. The pot experiment further proves that the disease index of the cotton verticillium wilt and the biomass of pathogenic bacteria at the root can be remarkably reduced by inoculating the strain, the growth promoting effect is remarkable, and the root development and plant growth of crops can be remarkably promoted by applying the strain to the crops. The invention provides a special'growth-promoting and disease-resisting 'functional strain resource for crops such as cotton and the like, and has important significance.

The present application relates to the technical field of pharmaceutical chemistry, and particularly relates to application of tryptophanone in prevention and treatment of plant bacteria and part of plant fungi. The present application discloses that natural product tryptophanone can effectively prevent and treat plant bacterial and part of plant fungal diseases. The application of tryptophanone as an agricultural bactericide in plant bacterial diseases has excellent inhibitory effect on rice bacterial leaf blight, rice bacterial leaf streak, citrus canker, cabbage black rot, tomato bacterial spot, kiwi canker, tobacco bacterial wilt, cucumber bacterial angular spot, potato bacterial wilt, soybean bacterial spot, tomato canker and the like. The tryptophanone also shows good inhibitory activity on plant pathogenic fungi such as grapevine Eutypa lata, tobacco alternaria alternata, eggplant verticillium wilt, pepper fusarium wilt, colletotrichum gloeosporioides, tea tree colletotrichum gloeosporioides and sorghum zylosporium.

This invention relates to the field of medicinal chemistry, specifically to a capsaicin-like compound containing isopropanolamine, its preparation method, and its application. This type of compound has a structure as shown in general formula (I). The invention uses capsaicin and isopropanolamine as pharmacophores, connecting the two pharmacophores with different bridging chains to synthesize a series of capsaicin-like derivatives containing isopropanolamine. These compounds exhibit excellent inhibitory effects on plant pathogenic bacteria such as rice bacterial blight, citrus canker, rice bacterial leaf streak, and kiwifruit canker; and plant pathogenic fungi such as cucumber gray mold, pepper wilt, rapeseed sclerotinia rot, eggplant verticillium wilt, wheat scab, potato late blight, blueberry root rot, grape coccidioidomyces, dragon fruit anthracnose, and rice sheath blight, providing an important scientific basis for the research and development of new pesticides.

The invention provides bacillus stutzeri HMB39561 and application thereof, and belongs to the technical field of agricultural microorganisms. The bacillus stutzeri HMB39561 disclosed by the invention is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number of the bacillus stutzeri HMB39561 is CGMCC No.37015. The strain not only has a good prevention and treatment effect on cotton damping-off and verticillium wilt, but also can increase the cotton yield and improve the cotton quality. The bacillus stutzeri HMB39561 disclosed by the invention can be used for green prevention and control of cotton damping-off and verticillium wilt, and has a relatively good application prospect in the field of cotton planting.

The application relates to the technical field of plant grafting, and particularly discloses a grafting method capable of improving disease resistance of eggplants, which comprises the following steps: Solanum nigrum seedling, eggplant seedling, seedling period management and grafting; in the application, the wild Solanum nigrum is selected as the stock, has the advantages of fast growth speed and good disease resistance, and can greatly improve the disease resistance and yield of the plant as the stock; the eggplant has a high incidence rate during the growth process, especially the occurrence of the yellow wilt disease is serious, and the yield is completely lost in serious cases; the grafting can solve the fundamental problem; the Solanum nigrum seed has a large amount, is easy to breed, and has a high germination rate; the special nutrient solution is sprayed, the wound healing speed of the grafting is greatly accelerated, and the healing effect is good; the grafting method of the sleeve pipe is adopted, the grafting speed is improved, the wound is protected, and the bacteria are prevented from invading the wound.

The application discloses application of a small G protein regulator VdRasGAP of Verticillium dahliae in resisting cotton verticillium wilt, and provides application of inhibition of expression and / or activity of the VdRasGAP protein in any of the following aspects: (A1) reducing pathogenicity of the Verticillium dahliae; (A2) resisting plant verticillium wilt caused by the Verticillium dahliae infection. The application lays a theoretical foundation for in-depth research on pathogenic mechanism of the Verticillium dahliae, provides a new theoretical basis and an effective target for efficient prevention and treatment of the cotton verticillium wilt, and lays a foundation for further development of a new type of target fungicide.

The invention discloses a sea island cotton SNP (Single Nucleotide Polymorphism) molecular marker and application thereof, and relates to the technical field of cotton molecular breeding, and the key points of the technical scheme are as follows: the SNP molecular marker is GbMA03G0171SNP; the nucleotide sequence of the GbMA03G0171SNP molecular marker is as shown in SEQ ID No.1, the polymorphic site of the GbMA03G0171SNP molecular marker is located at the 2195th site of the sequence as shown in SEQ ID No.1, and the polymorphism of the GbMA03G0171SNP molecular marker is A / T.

The application discloses application of a calcium-dependent protein kinase gene GhCDPK29 in plant resistance to verticillium wilt, and belongs to the technical field of genetic engineering. The gene GhCDPK29 is amplified by using sequence information of the gene, a VIGS (Virus Induced Gene Silencing) plant expression vector is constructed, the VIGS plant expression vector is transformed into Gossypium hirsutum TM-1, and transgenic cotton is obtained; after inoculation of cotton verticillium V991, the transgenic cotton shows resistance to verticillium wilt, which indicates that the gene GhCDPK29 is highly related to the resistance of cotton to verticillium wilt. The application lays an application foundation for research on a molecular mechanism of verticillium wilt resistance, cultivation of new plant varieties with verticillium wilt resistance, and provides a new reference for screening of new disease-resistant genes and cultivation of disease-resistant plants.

The invention relates to the field of biotechnology application, and discloses a GhHSL1 gene and application thereof in regulation and control of upland cotton verticillium wilt resistance. The nucleotide sequence of the GhHSL1 gene upland cotton genetic standard line TM-1 is shown as SEQ ID NO. 1, and the amino acid sequence of the GhHSL1 gene upland cotton genetic standard line TM-1 is shown as SEQ ID NO. 2. The gene is induced by verticillium dahlia to realize obvious up-regulation expression. According to the invention, a virus-induced gene silencing technology and a transgenic overexpression technology are further utilized to preliminarily research the function of the cotton GhHSL1 gene in cotton verticillium wilt resistance, and the result shows that the verticillium wilt resistance of cotton plants is obviously reduced after the GhHSL1 gene in upland cotton is silenced; after the GhHSL1 gene is over-expressed in upland cotton, the verticillium wilt resistance of the cotton is remarkably improved, and it is determined that the GhHSL1 gene positively regulates the resistance of the upland cotton to verticillium dahliae; the invention discloses the application of the GhHSL1 gene in regulating and controlling the disease resistance of upland cotton verticillium wilt for the first time, and provides theoretical guidance and genetic resources for improving the disease resistance of cotton through molecular design breeding or genetic engineering.

This invention belongs to the field of fermentation engineering and plant disease control technology, specifically relating to the application of a Bacillus endophyticus compound inoculant fermentation broth in the control of plant diseases. The Bacillus endophyticus compound inoculant disclosed in this invention exhibits antibacterial effects against anthracnose in peppers, blight in peppers, gray mold in tomatoes, wilt in watermelons, wilt in cucumbers, verticillium wilt in cotton, black spot in peppers, root rot in carrots, soft rot in taro, and bacterial wilt in peppers, with inhibition rates of (75.37±1.44), (71.00±1.41)%, (83.14±0.96)%, (74.70±1.09)%, (69.21±1.04)%, (78.31±1.39)%, (73.06±0.73)%, (75.49±0.89)%, (46.79±1.57)%, and (73.82±0.51)%, respectively.

The application discloses an application of a leucine-rich repeat receptor-like kinase gene of Gossypium anomalum, which is composed of 3885 bases, wherein the first base from the 5' end is a transcription start site, and the 3885th base is a transcription termination site. Expression of the gene can cause a lethal phenotype of a single segment introgression line CSSL11-9 of the Gossypium anomalum. The lethal phenotype is inhibited by silencing the gene expression in the single segment introgression line CSSL11-9 of the Gossypium anomalum, and the single segment introgression line CSSL11-9 can survive normally. In addition, the gene can also endow cotton with resistance to verticillium wilt and fusarium wilt, and the resistance of the CSSL11-9 plant with the silenced gene to the cotton verticillium wilt and fusarium wilt is significantly reduced, thereby providing an important gene resource for cotton breeding against the verticillium wilt and the fusarium wilt.