233 results about "Verticillium wilt" patented technology

The invention relates to the field of agricultural disease intelligent detection and computer vision interdiscipline, in particular to a cotton leaf verticillium wilt segmentation model based on a YOLO11 framework, and adopts the technical scheme that Swin Transform is adopted as a feature extraction module, and a layered framework and a sliding window mechanism are introduced; a multi-scale feature fusion attention mechanism is adopted as an attention mechanism module, features of different scales are extracted by using five different branches, then a comprehensive feature map is spliced in a channel dimension, and finally, a channel attention mechanism and a space attention mechanism are used to calibrate the merged feature map in a parallel mode. A sliding weighted loss function is adopted as a loss function; wherein the step of calibrating the merged feature map comprises the steps of obtaining an enhanced feature map through fusion after channel weighting and space weighting are achieved, and generating a final output feature map through dimensionality reduction and integration.

The invention relates to the technical field of biology, in particular to a verticillium dahliae virulence gene, a verticillium dahliae virulence protein and application. The invention discloses a verticillium dahliae virulence gene VdPHO23like. The verticillium dahliae virulence gene VdPHO23like comprises a polynucleotide sequence for coding an amino acid sequence of SEQ ID NO: 3. According to the invention, expression of the gene is inhibited in a targeted manner through an RNA interference mediated gene silencing technology so as to weaken virulence of pathogenic bacteria, and an efficient, specific and environment-friendly comprehensive prevention and control strategy is provided for verticillium wilt of crops such as cotton.

The invention provides bacillus amyloliquefaciens LYZ1125, a biocontrol microbial inoculum and application of the biocontrol microbial inoculum. The preservation number of the bacillus amyloliquefaciens LYZ1125 is CGMCC (China General Microbiological Culture Collection Center) No.31716, and the preservation number of the bacillus amyloliquefaciens LYZ1125 is CGMCC No.31716. The bacillus amyloliquefaciens LYZ1125 disclosed by the invention has relatively good bacteriostatic activity on 11 pathogenic bacteria of six common diseases of alfalfa and verticillium dahliae (separated from oxytropis aureocauda plants) belonging to the same species and different species as the verticillium medicago, and pot experiment shows that the bacillus amyloliquefaciens LYZ1125 can promote the growth of alfalfa root systems and plants; the morbidity of the alfalfa verticillium wilt can be remarkably reduced, the good prevention and treatment effect on the alfalfa verticillium wilt is achieved, and an important theoretical basis is provided for biological prevention and treatment of the alfalfa verticillium wilt (V.alfalfae) LYZ0257).

The invention relates to a cotton leaf disease identification method and system based on multilayer context feature screening, and belongs to the field of computer vision. According to the ConvNeXt-based cotton leaf disease identification method, a cotton leaf disease data set is constructed, and a system capable of identifying cotton physalospora piricola, brown blotch, angular leaf spot, verticillium wilt, fusarium wilt, anthracnose and powdery mildew and returning early warning information and prevention and treatment measures is established. According to the method provided by the invention, the ConvNeXt network is used as a backbone network, a feature enhancement module is added, the contrast among different regional features of the cotton leaves is improved, and on the basis, a feature screening attention mechanism of feature screening guided by multilayer context information is introduced, so that more effective feature screening is realized, and experiments show that the feature screening attention mechanism is more accurate. Compared with the recognition accuracy of the original ConvNeXt network, the recognition accuracy of the network model provided by the invention is improved by 4.1%.

The invention discloses and relates to a strain capable of resisting saline and alkaline and producing IAA (Indoleacetic Acid). The strain is classified and named as bacillus atrophaeus, and the preservation number of the strain is CGMCC (China General Microbiological Culture Collection Center) NO.29799. The strain is preserved in China General Microbiological Culture Collection Center on January 29, 2024. The bacillus atrophaeus HHQGTS13-1 can grow under the condition that the salt content is higher than 3%, can generate indoleacetic acid, cellulase, amylase, protease and ligninase, can inhibit various plant pathogenic bacteria, such as banana anthracnose, tomato gray mold, cotton verticillium wilt, rice sheath blight disease, wheat head blight, banana fusarium wilt, capsicum white spot and the like, and can be used for preparing the bacillus atrophaeus HHQGTS13-1. Particularly, the compound plays an important role in preventing and treating wheat scab, banana anthracnose and capsicum white spot. The strain can promote plant growth in a saline-alkali environment, and can be used for improving soil quality, developing special microbial fertilizers, microbial agents and biopesticides for saline-alkali soil, relieving crop salt stress damage and increasing crop yield.

The invention provides a cotton verticillium wilt resistance detection method based on an unmanned aerial vehicle chlorophyll detection model, relates to the technical field of data detection, and solves the technical problem of low cotton verticillium wilt resistance detection efficiency. The method comprises the following steps: acquiring RGB data and multispectral data of a specified field area of cotton through image acquisition equipment arranged on an unmanned aerial vehicle; based on the RGB digital orthoimage, the multispectral digital orthoimage and chlorophyll content data measured on site by different varieties of cotton, utilizing a machine learning algorithm to construct chlorophyll detection models of chlorophyll of different varieties of cotton in different growth periods by adjusting initial model parameters; based on the optimal chlorophyll detection model and the verticillium wilt disease index data, K-means clustering analysis and GIS space analysis are carried out, chlorophyll change rule data and verticillium wilt resistance levels of different varieties of cotton are obtained, and the verticillium wilt resistance levels of different varieties of cotton serve as cotton verticillium wilt resistance detection results.

The invention provides a purple blue-like bacterium and an application of the purple blue-like bacterium in prevention and treatment of cotton diseases. The preservation number of the purple blue-like bacterium is CCTCC (China Center For Type Culture Collection) NO: M 20242093. On the other hand, the invention provides an application of the blue purple-producing bacterium CEF642N strain, namely the application of the blue purple-producing bacterium CEF642N strain serving as a biological control agent for cotton verticillium wilt. According to the application disclosed by the invention, the inhibition effect of the purple blue-like bacterium CEF642N extract component on verticillium dahliae is analyzed, and the purple blue-like bacterium CEF642N extract has the effect of preventing and treating the verticillium wilt and can be used for preparing a biological prevention and treatment agent, so that a new biological prevention and treatment means is provided for preventing and treating the verticillium wilt.

The invention discloses an upland cotton GhCRK29 gene as well as an encoding protein and application thereof, the GhCRK29 gene is separated from cotton, the nucleotide sequence of the GhCRK29 gene is as shown in SEQ ID No.1, and the amino acid sequence of the encoding protein of the GhCRK29 gene is as shown in SEQ ID No.2. According to the invention, a virus-induced gene silencing technology and a transgenic overexpression technology are further utilized to carry out preliminary research on the function of the cotton GhCRK29 gene in cotton verticillium wilt resistance, and a result shows that the verticillium wilt resistance of a cotton seedling plant is obviously reduced after the GhCRK29 gene in cotton is silenced; after the GhCRK29 gene is over-expressed in arabidopsis thaliana or cotton, the verticillium wilt resistance of arabidopsis thaliana or cotton is remarkably improved, and the resistance of a GhCRK29 gene forward regulation plant to verticillium dahliae is determined; the invention has application prospects in the aspects of improving the verticillium wilt resistance of plants or cultivating verticillium wilt resistant plant varieties and the like.

The invention provides a cotton verticillium wilt early detection method based on a hyperspectral imaging recurrence plot, which comprises the following steps: inoculating cotton verticillium wilt germs to obtain healthy plants, non-symptomatic plants and symptomatic plants; acquiring and processing hyperspectral images in different verticillium wilt states; converting the one-dimensional hyperspectral curve into a two-dimensional recurrence plot (RP); performing graying processing on the two-dimensional recursive image; extracting a gray gradient co-occurrence matrix (GLGCM) of different images; carrying out Pearson correlation analysis, and evaluating the correlation between the texture features and the three types of labels; eliminating redundant information of the features by adopting principal component analysis (PCA), and obtaining a load matrix of each principal component; based on the four machine learning models, a three-classification cotton plant verticillium wilt early detection model of'healthy, non-symptom-symptom 'is established. According to the method, the feasibility of converting the spectrum curve into the recursion plot and extracting the image texture features for accurate identification of the cotton verticillium wilt in the asymptomatic period is proved, and reliable reference is provided for accurate identification of cotton verticillium wilt infection.

The invention provides bacillus atrophaeus LYZ1127, a biocontrol microbial inoculum and application of the biocontrol microbial inoculum. The preservation number of the bacillus atrophaeus LYZ1127 is CGMCC (China General Microbiological Culture Collection Center) No.31718, the preservation number of the bacillus atrophaeus LYZ1127 is CGMCC No.31718, and the preservation number of the biocontrol microbial inoculum is CGMCC No.31718. The bacillus atrophaeus LYZ1127 has relatively good bacteriostatic activity on 11 pathogenic bacteria of six common diseases of alfalfa and verticillium dahliae (separated from oxytropis aureocauda plants) belonging to the same genus and different species with verticillium wilt of alfalfa, and pot experiment shows that the bacillus atrophaeus LYZ1127 can promote the growth of alfalfa root systems and plants, can also remarkably reduce the incidence rate of verticillium wilt of alfalfa, and can be applied to the field of agricultural chemical engineering, such as agricultural chemical engineering, agricultural chemical engineering, agricultural chemical engineering, agricultural chemical engineering, agricultural chemical engineering, agricultural chemical engineering, agricultural chemical engineering, agricultural chemical engineering, agricultural chemical engineering, agricultural chemical engineering, agricultural chemical engineering, agricultural chemical engineering, agricultural chemical engineering and agricultural chemical engineering. The method has a good control effect on the verticillium wilt of the alfalfa, and provides an important theoretical basis for biological control of the verticillium wilt of the alfalfa.

The invention discloses bacillus velezensis BR-1 and application of the bacillus velezensis BR-1 in prevention and treatment of economic crop diseases. The preservation number of the bacillus velezensis BR-1 is CCTCC NO: M 20251379. The bacillus velezensis BR-1 disclosed by the invention is high in adaptability and wide in growth condition range, and is more beneficial to application in agricultural production. Meanwhile, the bacillus velezensis BR-1 has a remarkable inhibition effect on the growth of various phytopathogens such as verticillium dahliae, fusarium oxysporum, sclerotinia sclerotiorum and ralstonia solanacearum, can remarkably reduce the morbidity of cotton verticillium wilt, fusarium wilt and tomato bacterial wilt under a greenhouse condition, and has a wide agricultural application prospect.

The invention relates to the technical field of microorganisms, and particularly provides application of bacillus amyloliquefaciens BA-89 in crop growth promotion and verticillium dahliae antagonism. The strain is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.36498. The strain can secrete multiple extracellular enzymes (including protease, amylase, cellulase and glucanase), has the growth promoting characteristics of producing ammonia, dissolving potassium and the like, and can tolerate high temperature and ultraviolet stress; the compound has a remarkable antagonistic effect on various plant pathogenic bacteria (such as verticillium dahliae, fusarium oxysporum and phytophthora capsici); by inoculating the strain on cotton, the plant growth can be obviously promoted, the plant height, stem diameter, root length, leaf area and biomass are improved, and meanwhile, the disease index of verticillium wilt and the colonization amount of pathogenic bacteria at the root are effectively reduced. The strain disclosed by the invention can be used as a biological fertilizer and a biological pesticide, is used for cotton cultivation and disease prevention and control, and has a wide application prospect.

The invention belongs to the technical field of agricultural microbial control, and particularly relates to a growth-promoting and disease-preventing compound microbial agent and a preparation method and application thereof, and the compound microbial agent specifically comprises microbial powder, a growth auxiliary material and a drying agent; the microorganisms comprise vermiform yellow, trichoderma harzianum, bacillus velezensis, bacillus licheniformis and brevibacillus laterosporus. According to the compound microbial agent, through the synergistic effect of multiple strains, the control effect of the compound microbial agent on verticillium dahliae is enhanced, and the defects that a single biocontrol bacterium is single in biocontrol mechanism, poor in environment adaptability, incapable of being colonized in soil for a long time and unstable in control effect are overcome; the compound microbial agent can increase the yield of cotton and pepper and improve the quality of cotton fibers, and provides scientific and technological support and decision service for realizing green, environment-friendly, cost-saving and efficiency-increasing modern agriculture.

The invention provides a composite flora for preventing and treating cotton verticillium wilt and a microbial agent and application thereof, and belongs to the technical field of agricultural microorganisms. The composite flora and the microbial agent provided by the invention comprise pseudoarthrobacter oxydans CRB-17 and bacillus velezensis CRB-46; the preservation numbers of the pseudoarthrobacter oxydans CRB-17 and the bacillus velezensis CRB-46 are respectively CCTCC (China Center for Type Culture Collection) NO: M20252254 and CCTCC NO: M20252255, and the pseudoarthrobacter oxydans CRB-17 and the bacillus velezensis CRB-46 are both preserved in the China Center for Type Culture Collection on October 20, 2025. The pseudoarthrobacter oxydans CRB-17 and the bacillus velezensis CRB-46 are stably colonized in the rhizosphere of the cotton, have a strong direct antagonism effect on verticillium dahliae, can effectively and continuously prevent and treat the verticillium wilt of the cotton, effectively promote the germination of cotton seeds, the elongation of radicles and hypocotyls, promote the growth of the cotton and improve the defensive power of the cotton.

The invention belongs to the technical field of gene cotton resistance breeding, and particularly relates to application of cotton GhGSTF8 and GbGSTF8 genes in cotton verticillium wilt resistance. According to the application, the expression level of the GhGSTF8 gene in leaves and receptacles of cotton is relatively high through qRT-PCR (Quantitative Reverse Transcription-Polymerase Chain Reaction) detection; the expression level of the GbGSTF8 gene in leaves and stems of cotton is high. VIGS experiments show that after the GhGSTF8 gene is silenced, a new plant body shows an obvious susceptible character characteristic to verticillium wilt infection. After the GbGSTF8 gene is silenced, the silenced plant shows the character characteristic of resistance. Based on the research result, a certain application foundation can be laid for the molecular mechanism research of verticillium wilt resistance and the cultivation of new varieties of novel verticillium wilt-resistant plants.

The invention discloses bacillus velezensis with antagonism on cotton verticillium wilt and high salt stress resistance and application, the strain has the advantages of resisting saline and alkaline and antagonizing the cotton verticillium wilt at the same time, the problem of verticillium wilt in the existing saline-alkali soil cotton planting process is solved, the utilization of saline-alkali soil is expanded, and the cotton yield is increased.

The invention belongs to the field of biological detection, and particularly relates to a kit and a method capable of doubly detecting verticillium dahliae and fusarium oxysporum. The optimal primer pair aiming at verticillium dahliae and fusarium oxysporum is found, an RPA single detection method and an RPA dual detection method are established based on the primer pair, and the RPA-LFD primer pair is suitable for RPA-LFD detection. Compared with the common technology for detecting the verticillium wilt at present, the method has the advantages that high specificity and low detection limit can be ensured, verticillium dahliae and fusarium oxysporum in related cotton samples can be detected more sensitively, quickly and conveniently, dependence on professional technicians and laboratory precise instruments can be avoided, and the method is suitable for large-scale popularization and application. The method is of great significance to rapid and accurate identification, monitoring and early prevention and treatment of cotton verticillium wilt and fusarium wilt.

The invention discloses a verticillium dahliae virulence gene, a verticillium dahliae virulence protein and application. The invention relates to the technical field of biology, and provides a method for preventing and treating cotton verticillium wilt based on RNAi (RNA interference) as well as related components and application thereof. Specifically, the invention discloses application of the verticillium wilt prevention and treatment by a method for inhibiting or silencing the expression of verticillium dahliae pathogenic gene VdHDAC (coding protein of the verticillium dahliae pathogenic gene VdHDAC is shown as SEQ ID NO: 3), the core of the verticillium wihliae pathogenic gene VdHDAC is to provide dsRNA molecules capable of targeting the gene, and the positive-sense strand of the dsRNA molecules is preferably selected from SEQ ID NO: 5, SEQ ID NO: 8, SEQ ID NO: 9 or SEQ ID NO: 11. Based on the molecule, the invention further provides a recombinant vector containing the coding sequence, a recombinant microorganism, a transgenic disease-resistant plant and an RNAi pesticide preparation. The scheme can be realized through host-induced gene silencing, microorganism-mediated gene silencing or preparation treatment and the like, the virulence of pathogenic bacteria can be effectively reduced, and a new technical approach is provided for green prevention and control of verticillium wilt.

The invention belongs to the technical field of microorganisms, and particularly relates to predatory tubulomyces sp. And application of the predatory tubulomyces sp. In biological prevention and control of plant diseases. The predatory tubulomyces sp. Strain NSE202 is preserved in China General Microbiological Culture Collection Center (CGMCC) on January 3, 2023, and the preservation number is CGMCC NO.26375. The invention further discloses a preparation method of the predatory tubulomyces sp. Strain NSE202. According to the present invention, the growth of a variety of plant pathogens such as Erwinia amyloliquefaciens, pectobacterium carotovorum subsp. Carotovora, pear rust pathogen and the like is inhibited through the predation manner, and the broad-spectrum pathogen resistance of the tubulomyces sp. NSE202 is represented; meanwhile, the tubulomyces sp. Strain NSE202 can effectively inhibit the infection of pear fire blight bacteria and cotton verticillium wilt bacteria on pyrus betulaefolia and cotton, reduces the morbidity of pear fire blight and cotton verticillium wilt, and can be used for biological control of plant diseases caused by phytopathogens.

The invention discloses a TLP1-like gene molecular marker related to cotton verticillium wilt resistance and application of the TLP1-like gene molecular marker, and belongs to the field of biotechnology application. The promoter of the TLP1 protein gene TLP1-like has two haplotypes, the promoter sequence of the haplotype 1 is SEQ ID NO.5, and the genomic sequence of the haplotype 2 is SEQ ID NO.6. The invention also discloses a method for preparing the TLP1 protein gene TLP1-like promoter of the TLP1 protein gene TLP1-like promoter of the TLP1 protein gene. Compared with the haplotype 1, the haplotype 2 has an SV insertion of 2623 bp at a-899 bp position of an ATG upstream promoter region of a TLP1-like gene, so that the expression quantity of the TLP1-like gene is reduced. An InDel functional marker capable of remarkably distinguishing the haplotype 1 and the haplotype 2 is further developed. Compared with the haplotype 1, the haplotype 2 has a remarkable advantage in the aspect of improving the verticillium wilt resistance of the cotton, and the silent haplotype 1 cotton plant TLP1-like remarkably improves the resistance of the cotton to verticillium dahliae. The haplotype 2 has important effects and application prospects in screening cotton disease-resistant materials and cultivating cotton high-resistance varieties.

The invention discloses a cotton GhATG1t gene and application of the cotton GhATG1t gene in regulation and control of verticillium wilt resistance of plants. According to the invention, the GhATG1t gene is separated from cotton, the nucleotide sequence of the GhATG1t gene is shown as SEQ ID No.1, and the amino acid sequence of the encoded protein of the GhATG1t gene is shown as SEQ ID No.2. According to the invention, a virus-induced gene silencing technology and a transgenic overexpression arabidopsis thaliana technology are further utilized to carry out preliminary research on the function of the cotton GhATG1t gene in cotton verticillium wilt resistance, and a result shows that after the GhATG1t gene in cotton is silenced, the verticillium wilt resistance of a cotton seedling plant is obviously reduced; after the GhATG1t gene is over-expressed in arabidopsis thaliana, the verticillium wilt resistance of arabidopsis thaliana is remarkably improved, and the resistance of a GhATG1t gene forward regulation plant to verticillium dahliae is determined; the invention has application prospects in the aspects of improving the verticillium wilt resistance of plants or cultivating verticillium wilt resistant plant varieties and the like.

The invention discloses a cotton GhLAC14-3 gene and application of the cotton GhLAC14-3 gene in regulation and control of verticillium wilt resistance of plants. According to the invention, the GhLAC14-3 gene is separated from cotton, the nucleotide sequence of the GhLAC14-3 gene is shown as SEQ ID No.1, and the amino acid sequence of the encoded protein of the GhLAC14-3 gene is shown as SEQ ID No.2. The invention further performs preliminary research on the function of the cotton GhLAC14-3 gene in cotton verticillium wilt resistance, and the result shows that after the GhLAC14-3 gene in cotton is silenced, the verticillium wilt resistance of a cotton seedling plant is obviously reduced; after the GhLAC14-3 gene is over-expressed in arabidopsis thaliana, the verticillium wilt resistance of the arabidopsis thaliana is remarkably improved, so that the resistance of a GhLAC14-3 gene positive regulation plant to verticillium dahliae is determined. The invention has application prospects in the aspects of improving the verticillium wilt resistance of plants or cultivating verticillium wilt resistant plant varieties and the like.

The invention relates to a verticillium wilt resistance gene Gbar4CL1 of cotton and application of the verticillium wilt resistance gene Gbar4CL1. The gene of a cotton verticillium wilt resistance material M34 is subjected to map-based cloning, the cotton verticillium wilt resistance gene Gbar4CL1 is identified for the first time, the Gbar4CL1 gene is proved to be highly expressed after being induced by verticillium wilt, and further the gene is over-expressed in cotton to verify that the gene can obviously enhance the verticillium wilt resistance of the cotton, so that the cotton verticillium wilt resistance of the cotton can be obviously enhanced. The gene has important value for theoretical researches such as analysis of molecular mechanisms of cotton verticillium wilt resistance and the like. The Gbar4CL1 gene can improve the resistance of cotton to verticillium wilt, and has important practical application significance for cotton verticillium wilt resistant breeding.

The invention belongs to the technical field of microorganisms, and discloses bacillus velezensis and application thereof in plant disease control. A bacillus velezensis strain is separated and screened from rhizosphere soil of a wolfberry plant in a Nomuliflood farm in Qinghai province, the strain is bacillus velezensis G3 and is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No. 36136. The bacterial strain has a better antibacterial effect on pathogenic bacteria fusarium oxysporum of Chinese wolfberry root rot, also has an inhibiting effect on pathogenic bacteria of Chinese wolfberry verticillium wilt, botrytis cinerea, sclerotinia rot of colza, alternaria alternata, apple anthracnose, apple rot, pear wilt and wheat scab, and has broad-spectrum antibacterial activity; wide application prospects are realized in plant disease prevention and treatment.

The invention relates to the technical field of agricultural biology, in particular to a verticillium dahliae heat shock protein 90 ATP enzyme activating factor VdAHA1 as well as a coding gene and application of the verticillium dahliae heat shock protein 90 ATP enzyme activating factor VdAHA1. The invention provides a verticillium dahliae heat shock protein 90 ATP enzyme activating factor VdAHA1 related to high temperature resistance, drug resistance, environmental adaptability, ergosterol synthesis capability and pathogenicity of verticillium dahliae and a coding gene of the VdAHA1, and the VdAHA1 gene positively regulates the pathogenicity of verticillium dahliae and can be used as an important candidate gene for pathogenesis research of verticillium dahliae. The invention provides a theoretical basis for further understanding the pathogenic mechanism of pathogenic bacteria, also provides a new gene resource for verticillium dahliae pathogenic related genes, also provides a new idea for prevention and control of cotton verticillium wilt, and has a wide application prospect.

The invention discloses an application of a GhMKK5 gene in improving the disease resistance of cotton verticillium wilt, and belongs to the field of biotechnology application. The GhMKK5 gene disclosed by the invention is used for coding a mitogen activated protein kinase kinase. The invention provides a full-length CDS nucleotide sequence and an amino acid sequence of GhMKK5 in an upland cotton genetic standard line TM-1. The gene is expressed in all tissues of cotton, and is induced by verticillium dahliae to realize obvious up-regulation expression. The verticillium wilt resistance of cotton is reduced by silencing the gene in cotton, and the disease resistance of arabidopsis thaliana is improved by overexpressing the gene in arabidopsis thaliana, which indicates that the gene plays an important role in cotton verticillium wilt resistance.

The invention discloses application of a cotton GbbHLH35 gene in improving the disease resistance of plant verticillium wilt, the nucleotide sequence of the gene is shown as SEQ ID NO: 1, and the amino acid sequence is shown as SEQ ID NO: 2. The gene is remarkably and highly expressed in cotton roots and is remarkably up-regulated under the induction of verticillium wilt, and the sequence of the gene contains a conserved motif related to disease resistance. The sensitivity of the plant to verticillium wilt is enhanced by silencing the expression of the GbbHLH35 gene in the sea island cotton. The overexpressed GbbHLH35 gene arabidopsis thaliana homozygous line is obtained through a transgenic technology, and after the overexpressed GbbHLH35 gene arabidopsis thaliana homozygous line is infected with verticillium The invention has important significance in improvement of cotton verticillium wilt resistance breeding and research, and can be applied to breeding of resistant cotton varieties.

The invention belongs to the technical field of biological prevention and control of plant diseases, and provides application of bacillus subtilis in prevention and control of verticillium wilt. The preservation number of the bacillus subtilis SEB-34 is CGMCC (China General Microbiological Culture Collection Center) No. 26419. The SEB-34 strain and the microbial agent produced by the SEB-34 strain can be used for preventing and treating plant diseases caused by verticillium dahliae, and the strain can be colonized at plant roots and keep high abundance and has the effects of preventing diseases, promoting growth and increasing yield. In the microbial agent provided by the invention, thalli are in a semi-dormant state, so that the microbial agent has relatively strong stress resistance and is more stable to store, and the production and transportation costs are effectively reduced while the effect is ensured. The invention also provides beneficial microbial resources for green prevention and control of economic crop diseases, and has good application prospects and high application values.

The invention relates to the technical field of plant genetic engineering, and particularly provides cloning of a GbGELP25D gene of gossypium barbadense and application of the GbGELP25D gene of gossypium barbadense. According to the invention, the GbGELP25D gene with a full length of 1092 bp is cloned from the sea island cotton variety Xinhai No. 7 for the first time, and the gene encodes a secretory lipase protein with a signal peptide and is positioned in an extracellular gap. Through bioinformatics analysis, phylogenetic classification, protein structure modeling and signal peptide function verification, it is clear that the gene belongs to a plant GELP family. Furthermore, a virus-induced gene silencing technology is utilized to prove that the silent GbGELP25D can obviously enhance the resistance of cotton to verticillium wilt, and the mechanism of the silent GbGELP25D is closely related to activation of ethylene synthesis and signal channels and induction of expression of disease-resistant related genes. The resistance gene provided by the invention enriches gene resources of cotton verticillium wilt resistance breeding, and has important theoretical significance and application value.

The invention provides a charcoal fungicide for preventing and treating cotton verticillium wilt and a preparation method of the charcoal fungicide, and belongs to the technical field of agricultural fungicide. According to the invention, the agricultural waste walnut green seedcase can be fully utilized to prepare the biochar material, and the biochar not only has good adsorption performance, but also is prepared through one-step pyrolysis, so that the process is simpler. The biochar is rich in functional groups, relatively high in specific surface area and relatively high in adsorption capacity, has a wide application prospect in the aspect of microbial agents, is good in prevention and treatment effect, and has a wide application value in the aspect of biological prevention and treatment of cotton verticillium wilt.