1193 results about "Chromosome" patented technology

The invention relates to the technical field of plant breeding, in particular to a molecular marker related to oil content and protein content of soybean and application of the molecular marker. The molecular marker is constructed on the basis of SNP sites; based on a genome version number Wm82. A2. V1, the SNP site is located at the 8605814th site of the No.8 chromosome of the soybean, and the polymorphism is A / G. The application comprises the following steps: (1) predicting or detecting the oil content or protein content of soybeans; (2) identifying or cultivating soybean varieties with high oil content or protein content; (3) soybean molecular marker assisted breeding; (4) soybean variety improvement related to oil content or protein content; and (5) soybean germplasm resource improvement. The molecular marker related to the soybean oil content and the protein content is obtained through research and screening, and the molecular marker can be applied to cultivation of soybean varieties with high oil content and high protein content and has important value in the field of soybean breeding.

A molecular marker closely linked to a pepper fruit shape gene. Taking a basic version of Zhangshugang pepper as a reference gene, a single nucleotide polymorphism is present at base 162028839 of the pepper Chr02 chromosome, where a substitution from base C to A occurs. A primer and kit for identifying the molecular marker closely linked to the pepper fruit shape gene, and use thereof in identifying the pepper fruit shape or in molecular-assisted breeding. Early use of the molecular marker, the primer, and the kit in the early stage can quickly screen satisfactory plants, thereby effectively reducing the planting scale, reducing the workload of later identification, and improving the selection efficiency and accuracy. The molecular marker, the primer, and the kit are of great significance in the practice of pepper fruit shape breeding and the research on fruit shape changes and regulation mechanisms.

The invention discloses a molecular marker closely linked with a cowpea salt tolerance major QTL (quantitative trait loci) and a primer sequence of the molecular marker. The name of the cowpea salt tolerance major QTL is SC300126, a KASP type molecular marker 300126 closely linked with the cowpea salt tolerance major QTL is located at the position of 43816166 bp on a chromosome 11, the marker can be used for amplifying a material to be detected, and whether a sample carries key allelic variation for controlling salt tolerance or not is judged according to the genotype of the marker. The molecular marker and the primer provided by the invention can be used for quickly screening the cowpea salt tolerance major QTL, and compared with a traditional phenotype identification method, the molecular marker and the primer have the advantages of high detection flux, accurate and reliable result and the like, and the typing accuracy can reach 99% or above. The technology can significantly improve the breeding efficiency of the salt-tolerant variety of cowpea and shorten the breeding period.

The invention relates to the technical field of plant genetic engineering, and discloses a haplotype identification marker of a rice flood-resistant gene ARF9 and application of the haplotype identification marker. The molecular marker is derived from an exon of an ARF9 gene, is located at the 22009082bp and 22010019bp of a No.4 chromosome of rice, and has a basic group of C. The SNP molecular marker is related to the waterflooding resistance of a male parent oryza longistaminate of perennial rice, and the waterflooding resistance of the male parent oryza longistaminate with a locus genotype of CC is obviously higher than that of rice with a locus genotype of non-AA type. Therefore, the invention provides the loop-mediated isothermal amplification primer group for rapidly identifying the ARF9 gene haploid molecular marker, and the loop-mediated isothermal amplification primer group is used for identifying the ARF9 gene haploid molecular marker so as to rapidly screen out waterflooding-resistant rice. The invention provides a group of primers, the flood-resistant rice can be quickly identified by adopting the primers to perform loop isothermal reaction, the detection cost is low, the detection speed is high, the flood-resistant germplasm can be quickly screened in the rice seedling stage by adopting the method provided by the invention, a time-consuming flooding test does not need to be performed in the later stage, and the breeding process is greatly accelerated.

The invention belongs to the technical field of animal molecular markers, and particularly relates to an SNP molecular marker for beef cattle flavor character breeding and application of the SNP molecular marker. The invention proposes that genotype polymorphism of 84181318bp and 84181699bp on chromosome 3 of an international bovine reference genome AR-UCD1.2 version is related to the character of the content of the beef arachidonic acid for the first time, the genotype polymorphism can be used for evaluating the content of the beef arachidonic acid, and beef cattle with specific genotypes at the two sites has relatively high content of the beef arachidonic acid. The site can be used as a molecular marker for evaluating the content of the arachidonic acid in the beef cattle, early prediction and auxiliary screening of beef cattle individuals with excellent meat quality and flavor characters, and a theoretical basis and a technical support are provided for breeding of the beef cattle with high content of the arachidonic acid in the beef cattle.

The invention belongs to the technical field of plant molecular markers, and particularly discloses an Indel molecular marker related to the salt tolerance of brassica napus and application of the Indel molecular marker. The marker is located at the position of an A01 chromosome scafoldA0133077947 of a brassica napus reference genome ZS11.v10, and the genotype of a polymorphic site is CTAA or C. The invention further discloses a method for identifying the marker of the brassica napus. Large-scale group phenotypic association analysis proves that the molecular marker is remarkably associated with the salt tolerance of the brassica napus in the germination period, and when the polymorphic site genotype is CTAA, the salt tolerance in the germination period is high; when the genotype of the polymorphic site is C, low salt tolerance in the germination period is shown. The invention finds that the polymorphic site is obviously associated with the salt tolerance of the brassica napus for the first time, provides a powerful molecular tool for solving the problems of difficulty in phenotype identification, long period and low efficiency in salt tolerance breeding of the brassica napus, can accurately screen salt-tolerant materials in the early stage, and greatly accelerates the breeding process of new varieties of the salt-tolerant brassica napus.

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker related to a Hu sheep body height character and application thereof, and belongs to the field of gene engineering. The SNP molecular marker is located at the nucleotide at the 71034104th site of the No.17 chromosome of the sheep reference genome AR-UIRambv3.0 version, the polymorphism of the site is T or C, and the genotype is TT, TC or CC. Correlation analysis verifies that the body height of a TT genotype Hu sheep individual is obviously higher than that of TC genotype and CC genotype individuals. The invention further provides a primer for detecting the SNP molecular marker and application of the primer in Hu sheep body height character auxiliary screening. By utilizing the molecular marker, dominant genotype individuals can be quickly and accurately identified in the young Hu sheep period without waiting for the Hu sheep to grow to the body size for determining the age, the breeding efficiency of the Hu sheep body height can be greatly improved, and the molecular marker has important significance for breeding large Hu sheep strains and improving the breeding economic benefits.

The present disclosure provides methods and systems for identifying transcriptional regulatory modules (e.g., in non-coding portions of the genome), predicting gene regulation and expression, e.g., effects of non-coding mutations or chromosome rearrangements on the regulation and expression of the target genes, and designing and using modified regulatory sequences.

The invention belongs to the field of pepper breeding, and discloses a KASP molecular marker closely linked with pepper fruit length, a primer, a kit and application, the molecular marker takes a Ca591.0 version gene as a reference gene, the single nucleotide polymorphism at the 70238018 position of the pepper No.3 chromosome is subjected to base A-T replacement, the flanking nucleotide sequence is shown as SEQ ID No: 1, the nucleotide sequence is shown as SEQ ID No: 2, and the nucleotide sequence is shown as SEQ ID No: 3. The sequences of the primers are shown as SEQ ID No: 2-4, and the kit comprises the primers. The invention also discloses an application of the primer or the kit in identification of different length types of pepper fruits or in molecular assisted breeding of pepper fruit length. A primer and a kit are developed aiming at the molecular marker, and can be used for identifying the length of the pepper fruit, so that assistant breeding is carried out by depending on the molecular marker, and the problems that the conventional breeding period is long and is easily influenced by the environment can be effectively solved.

The present invention encompasses compositions and methods to allow one to deliver and express multiple genes under the control of multiple gene regulatory components in a recipient cell via a synthetic chromosome. The engineering of synthetic chromosomes to contain multiple gene control units permits the construction of complex biological circuits.

The invention discloses an Indel marker related to a corn kernel protein content character and application of the Indel marker, and belongs to the technical field of plant molecular genetics and crop molecular design breeding. The Indel marker is located on a gdh2 gene of a chromosome 10 of a corn genome, and insertion or deletion of a nucleotide sequence as shown in SEQ ID NO.5 occurs in an intron region between a second exon and a third exon. By applying the molecular marker-assisted selection technology, high-protein new germplasms S3317 (the crude protein content is 17.0%) and JS231148 (the crude protein content is 15.6%) have been successfully cultivated, the increase amplitudes of the high-protein new germplasms and JS231148 are both more than 15% compared with those of the conventional control, and the lysine content reaches 0.41% and reaches the national high-quality protein corn standard.

The invention discloses a molecular marker InDel20 for identifying soybean oil content, a primer and application of the molecular marker InDel20. The molecular marker InDel20 is an insertion / deletion variation of 321 bp located at the 20th chromosome Chr20: 31728602-31728922 of the soybean, and the nucleotide sequence of the molecular marker InDel20 is as shown in SEQ ID No. 1; wherein the soybean material containing the insertion fragment is high-oil-content soybean, and the soybean material not containing the insertion fragment is low-oil-content soybean. The invention further provides a primer for detecting the molecular marker InDel20 and a method for identifying the content of soybean oil, and the identification accuracy rate is as high as 90.61%. The molecular marker InDel20 can be used for early-stage molecular marker-assisted selection of soybean high-oil characters, is suitable for germplasm resource screening, variety identification and molecular breeding of high-oil soybeans, and has important application value for accelerating polymerization of high-quality alleles and improving breeding efficiency.

The invention discloses a molecular marker related to slash pine paper pulp, a DNA (deoxyribonucleic acid) fragment, a kit and application of the molecular marker, the SNP molecular marker is S1641460596 and is located at the 641460596 site of a slash pine chromosome 1; the polymorphism of the SNP site is G / A, the genotype of the SNP site of the slash pine with the long wood fiber length is GG, and the genotype of the SNP site of the slash pine with the short wood fiber length is GA or AA. The DNA fragment of the gene is located in a Pel1G156900 gene region of a slash pine genome, and the nucleotide sequence of the gene is as shown in SEQ ID NO. 1 or SEQ ID NO. 2. The kit comprises a forward primer and a reverse primer which are used for amplifying the DNA fragment related to the slash pine paper pulp; the nucleotide sequence of the forward primer is as shown in SEQ ID NO.3, and the nucleotide sequence of the reverse primer is as shown in SEQ ID NO.4; the molecular marker, the DNA fragment and the kit are used for related phenotype identification, early prediction, breeding and the like of slash pine paper pulp, and the problems of late identification, long breeding period, low breeding efficiency and the like of existing slash pine paper pulp characters can be solved.

The invention discloses a structural variation molecular marker located on a pig chromosome 7 and related to the lactation ability of a sow. The structural variation molecular marker is a DNA (deoxyribonucleic acid) fragment which is inserted between the 112024896 bp site and the 112024896 bp site on a chromosome 7 of an international pig reference genome 11.1 version, wherein the nucleotide sequence of the DNA fragment is shown as SEQ ID NO: 1; the genotype of the gene is T / T, T / INS and INS / INS. The structural variation molecular marker provided by the invention obviously influences the total weight gain character and the lactation ability character of the 7-day-old sow, and by breeding the pig with the structural variation molecular marker genotype of T / T, the lactation ability of the sow can be obviously improved, and the survival rate and the weaning weight of piglets are increased.

The invention relates to the technical field of molecular markers, in particular to a molecular marker for early identification of immune traits of elephant male semi-fine wool sheep and application of the molecular marker. According to the present invention, the deterministic correlation between the genotype of the 102308292 site on the second chromosome and the immune globulin (IgA, IgG and IgM) level of the male semi-fine wool sheep is found for the first time; by detecting the genotype of the site, the future humoral immunity of the lamb can be predicted in the lamb stage, so that early seed selection is realized. The invention provides a powerful SNP marker for non-diagnostic purpose for disease-resistant breeding of male semi-fine wool sheep.

The invention relates to the field of genes, in particular to molecular markers for judging flowering time of brassica napus L. The molecular markers are Indel-282, Indel-326 and Indel-353 respectively and are located on an A02 chromosome of the brassica napus L.. The molecular marker has high specificity and stability, and is significantly related to the flowering time. The markers show high specificity and stability in brassica napus plants under different genetic backgrounds. By detecting the molecular markers through PCR amplification and electrophoresis, the flowering time of different plants can be quickly and accurately identified in the early stage of breeding. Therefore, the flowering time of the plant can be determined in the early stage, so that the breeding efficiency is improved. The molecular markers can be used for molecular marker-assisted selective breeding, and plants with early flowering or late flowering characteristics can be quickly screened by detecting the markers, so that the breeding process of excellent varieties is accelerated, and the functions and regulation mechanisms of related genes are further disclosed.

The invention discloses a QTL (Quantitative Trait Loci) influencing wheat plant height as well as a candidate gene TaMYB-A1 and application thereof. The QTL is QPh-7A-1534 and is located on a 7A chromosome, and the genetic interval of the QTL is 1528.3 cM to 1542.6 cM. A candidate gene exists on the QTL-QPh-7A-1534, specifically, the candidate gene is TaMYB-A1, and the nucleotide sequence of the candidate gene is as shown in SEQ ID No. 1. The candidate gene TaMYB-A1 is subjected to gene editing, and experiments prove that the TaMYB-A1 gene can influence the plant height of wheat, so that the genetic improvement and molecular breeding of the plant height character of wheat are facilitated, and the screening of wheat varieties can be accelerated to obtain dwarf wheat varieties.

The present disclosure provides a recombinant Escherichia coli strain modified by metabolic engineering means and a method for producing L-homoserine by using the same. The strain, designated as Escherichia coli having a strain number of 13-XA, is deposited in China General Microbiological Culture Collection Center (CGMCC) with an accession number of CGMCC No. 25099, dated Jun. 16, 2022. With respect to the chromosome DNA thereof, one or more genes associated with fatty acid metabolism are knocked out or attenuated, and / or a promoter is replaced for enhancement; one or more genes associated with the L-homoserine metabolic pathway are knocked out or attenuated, and / or one or more genes associated with the L-homoserine metabolic pathway are overexpressed or enhanced, and / or one or more genes associated with the L-homoserine metabolic pathway are mutated.

The invention belongs to the technical field of crop genetic breeding, and relates to a molecular marker linked with a peanut branch number related gene locus, an identification method and application of the molecular marker. The primer pair is C9-F / R, and the sequence of the primer pair is as shown in SEQ ID NO. 1 and SEQ ID NO. 2; by designing an InDel marker C9 linked with the number of branches, the character of the number of branches of peanuts can be quickly distinguished by using a primer to amplify a target band and using polyacrylamide gel electrophoresis detection. The identification method is more accurate and convenient, has good repeatability, can efficiently distinguish the phenotype and genotype of an individual, shortens the experiment process, has the advantages of simple and feasible operation steps and the like, and saves expenditure. The method disclosed by the invention has important application value in the breeding work aspects of peanut branch number character related gene positioning, genotype typing, later cultivation of excellent peanut varieties and the like.

The invention discloses application of TBR2 protein as well as a coding gene and a molecular marker thereof in resisting tomato brown wrinkled fruit virus diseases, and belongs to the technical field of gene engineering. According to the invention, a novel anti-disease gene TBR2 for resisting the tomato brown wrinkled fruit virus disease is identified on a chromosome 4 of tomatoes; the disease-resistant gene has stable disease resistance, and the resistance of the variety to the tomato brown crinkled fruit virus disease can be obviously improved by backcrossing and transbreeding the disease-resistant gene into a sensitive cultivar. Based on the anti-disease gene TBR2, the invention further develops molecular markers related to the tomato brown wrinkled fruit virus disease resistance, and the molecular markers can be used for identifying plant materials resistant to the tomato brown wrinkled fruit virus disease and cultivating plant varieties resistant to the tomato brown wrinkled fruit virus disease. The method is of great significance in accelerating the breeding process of disease-resistant tomatoes.

The invention belongs to the technical field of soybean molecular genetic breeding, and discloses a KASP marker for detecting an SNP site related to a soybean petiole included angle and application of the KASP marker. The SNP site is located at the 4589285th site on a chromosome 10 of a soybean reference genome Glycine max Wm82. A4. V1, and the site is subjected to C / T basic group replacement. Three KASP primers as shown in SEQ ID NO.2-4 are developed according to the SNP site, PCR amplification and genetic typing are performed on to-be-detected soybeans by using the primer group, and if a detection result shows that the genetic typing of the marker position is 1 / 1, it is judged that the petiole angle of the soybean variety is large; and if the detection result is 0 / 0, judging that the petiole angle is small. The SNP molecular marker can be used for molecular marker-assisted selection of soybean petiole included angle characters, and has important theoretical and practical guiding significance for accelerating genetic improvement of soybean compact plant types and improving breeding selection efficiency. The marker can rapidly identify the soybean petiole included angle, the method is simple and rapid, the identification result is accurate, and the marker has good popularization and application prospects.

The invention discloses a GmGID1-2 gene molecular marker obviously associated with soybean plant height, yield, grease content and protein content and application of the GmGID1-2 gene molecular marker. The molecular marker contains an SNP (Single Nucleotide Polymorphism) site obviously associated with soybean plant height, yield, grease content and protein content, the site is located in a GmGID1-2 gene promoter region, Wm82. A2. V1 is taken as a reference genome, the site is located at 36395036bp of a soybean No.3 chromosome, allelic variation is carried out to obtain a basic group C or T, and the nucleotide sequence is shown in SEQ ID NO: 1. Compared with the soybean with the genotype T, the soybean with the genotype C has the advantages of lower plant height, lower seed protein content, higher yield and higher seed oil content. The dCAPS molecular marker developed according to the SNP can be used for molecular marker-assisted selection, the soybean breeding efficiency is improved, and the soybean breeding process is accelerated.

The invention provides a chromosome instance segmentation method and system based on banding coding assistance, and the method comprises the steps: extracting chromosome key points through a multi-scale neural network, and obtaining a skeleton according with a topological structure through key point screening and curve fitting; a plurality of decoupling schemes are enumerated by calculating skeleton cross information, chromosome banding codes of all the schemes are matched with a standard coding library, and the optimal scheme is selected with the minimum editing distance; the optimal decoupling scheme and the multi-scale features are fused, the chromosome segmentation precision is improved through a segmentation model based on a cross attention mechanism, and high-quality instance masks and classification labels are output. The method can significantly improve the precision and robustness of chromosome instance segmentation, and can be widely applied to the medical diagnosis fields of genetic abnormality detection, blood disease detection and the like.

The invention discloses a molecular marker related to high-sugar feed utilization characters of siniperca chuatsi and application of the molecular marker. The molecular marker is located on a No.7 chromosome of siniperca chuatsi, and the molecular marker contains one SNP (Single Nucleotide Polymorphism) site slc25a11-1; the SNP site slc25a11-1 is located at the 1439370th basic group of the No.6 chromosome of siniperca chuatsi, and the polymorphic site is T / C. By implementing the molecular marker disclosed by the invention, the high-sugar feed utilization condition of siniperca chuatsi species can be remarkably improved, so that the feed cost is reduced, and the culture benefit is increased.

The invention discloses an amplification primer of a KASP marker on a D03 chromosome for cotton high lint breed breeding, which comprises a forward primer F1 with an FAM fluorescent marker, a reverse primer F2 with an FAM fluorescent marker, a reverse primer F2 with an FAM fluorescent marker, a reverse primer F2 with an FAM fluorescent marker, and a reverse primer F2 with an FAM fluorescent marker, the sequence of the forward primer F2 is shown as SEQ ID No. 2; and the sequence of the reverse primer is shown as SEQ ID No.3. The method can effectively distinguish genotypes with obvious lint content difference, and provides a useful tool for molecular marker-assisted selection in cotton breeding.

The invention discloses an anti-disease gene, an anti-disease protein, a molecular marker and application of tomato brown crinkled fruit virus disease, and belongs to the technical field of gene engineering. According to the invention, through a map-based cloning method, a new anti-disease gene TBR3 of the tomato brown fruit wrinkling virus disease is identified in a tomato chromosome 2 for the first time. The TBR3 gene is over-expressed in a plant body, so that the disease resistance of the plant to the tomato brown crinkled fruit virus disease is remarkably improved. Aiming at the disease-resistant gene TBR3, the invention also designs a molecular marker related to tomato brown crinkled fruit virus resistance, and tomato plants carrying a disease-resistant genotype and a susceptible genotype can be accurately distinguished, so that the molecular marker can be widely applied to early disease-resistant breeding and screening, and the problems of difficult early judgment and low breeding efficiency in a breeding process are solved.

The invention relates to the field of synthetic biology, metabolic engineering and fermentation engineering, and discloses a genetically engineered bacterium and application thereof in production of alpha-ketoglutaric acid.The genetically engineered bacterium comprises the steps that the genetically engineered strain with escherichia coli BW25113 or corynebacterium glutamicum ATCC13032 as a host is constructed; overexpression type isocitrate dehydrogenase genes are integrated through chromosomes, alpha-ketoglutarate dehydrogenase complex genes are knocked out, NADPH invertase is overexpressed through plasmids, and exogenous AKG transporter protein is introduced, so that metabolic flux directional regulation and control and product efflux enhancement are realized; meanwhile, a staged fermentation process is adopted and comprises dynamic gradient adjustment of inorganic phosphorus concentration and two-stage pH control. According to the method, the AKG yield of 78.2 g / L can be obtained under the 5L tank scale, the sugar acid conversion rate reaches 0.68, and the method has the advantages of being high in yield, low in byproduct and controllable in cost. The obtained AKG is suitable for a plurality of fields such as medical intermediates, functional foods and cell culture additives, and has wide industrial application prospects.

The invention discloses application of an SNP (Single Nucleotide Polymorphism) molecular marker related to the character of siniperca chuatsi in feeding artificial compound feed, and belongs to the field of biotechnology and aquatic animal genetic breeding. The SNP molecular marker is located at the 29390304 site from the 5'end on the 10 # chromosome of the siniperca chuatsi genome, is A or T, and is easy to domesticate when the genotype is AA; or the SNP molecular marker is located at the 1218488 site from the 5'end on the 20 # chromosome of the siniperca chuatsi genome, and is easy to domesticate when being the genotype of T or A or TT. The SNP molecular marker is closely related to the artificial compound feed intake capacity of the siniperca chuatsi, so that the feeding domestication potential of the siniperca chuatsi can be predicted in an early stage through the SNP molecular marker, or molecular marker-assisted breeding is performed through the SNP molecular marker, the proportion of individuals easy to domesticate in a group is increased, the breeding cost is remarkably reduced, and the breeding efficiency is improved. The economic benefit is improved.

The invention relates to the technical field of livestock molecular genetic breeding, in particular to application of a molecular marker for grassland short-tailed sheep body weight character breeding, the molecular marker is a nucleotide sequence as shown in SEQ ID NO.1, and nucleotide at the 101bp site is G or A; the application refers to any one of the following steps: identifying the weight of the grassland short-tailed sheep; the body weight of the grassland short-tailed sheep offspring is improved. The SNP molecular marker obviously related to the body weight character is identified on the 24 # chromosome of the grassland short-tailed sheep for the first time. The molecular marker can be used for genotype detection in a young sheep stage or an embryo stage, and does not need to wait for stable phenotype when the sheep grow to an adult stage, so that the breeding cycle is shortened, and the genetic progress is remarkably accelerated. The technical blank in the field of grassland short-tailed sheep body weight character molecular breeding is filled, and a reliable molecular tool and technical support are provided for efficient, accurate and sustainable breeding of the breed.

The invention relates to an SNP (Single Nucleotide Polymorphism) molecular marker closely linked with a pepper color value character and application of the SNP molecular marker, a nucleotide sequence of the SNP molecular marker comprises at least one of 254720000 base and upstream and downstream bases thereof and 254890442 base and upstream and downstream bases thereof on a pepper chromosome 3, the 254720000 base is marked as R1, the mutation type of the R1 is T or A, and the mutation type of the R1 is T or A; the 254890442nd base is marked as R2, and the mutation type of the 254890442nd base is C or T. The SNP molecular marker provided by the invention can be used for screening and identifying high-color-value pepper varieties, and the molecular marker-assisted selection breeding efficiency of target traits can be accelerated; the SNP molecular marker is directly expressed in the form of DNA, can be detected in each development stage and in different tissues and organs of the pepper, is not limited by environment and seasons, is not influenced by the problems of whether expression or not and the like, and has the advantages of simplicity in operation, accurate amplification result and lower cost of required reagents.