17 results about "Heredity" patented technology

The application provides a multi-source Parkinson's disease database integration method based on LEDD-UPDRS composite index alignment, and is applied to the technical field of data processing. In view of the heterogeneous problems of multi-source Parkinson's disease databases, the application integrates heterogeneous data of PPMI, PDBP and local queues, disassembles characteristics according to four modes of heredity and clinic, introduces a standardized term library and a BERT-BiLSTM-CRF model unified term adjusted in combination with medical corpus, generates a cross-library feature table; a storage architecture is constructed based on FlashROM mode to realize data storage and retrieval; a composite observation system is built with LEDD and UPDRS-III, consistency is verified by multiple methods, data is standardized, missing data is filled in combination with multiple models to generate an integrated data set; a double prediction model is built relying on the data set, and the advantages of the data are realized by a Cohen's d value verification method; finally, cross-library joint analysis is carried out, phenotype-genotype correlation modeling and causal inference are completed, and data support is provided for Parkinson's disease research and clinical prediction.

The invention relates to the technical field of alfalfa screening, discloses a high-quality and high-yield alfalfa hybrid combination screening method fusing multiple technologies, provides a multi-dimensional coding and double-elite evolution mechanism, constructs an improved non-dominated sorting genetic algorithm-II, and provides a high-quality and high-yield alfalfa hybrid combination screening method by coding a hybrid combination into a continuous real number vector. A chromosome expression mode decoupled from a problem structure is constructed, a double-elite mechanism of elite retention and offset mating is introduced, the retention and heredity ability of a high-quality solution is improved, offset crossover and jitter operation is used, premature convergence is avoided, efficient global search of a strain combination space is achieved, and a high-quality solution is obtained. Meanwhile, a multi-omics association model of genes, metabolites and phenotypes is provided, transcriptome, metabolome and molecular marker data are comprehensively utilized, a multi-dimensional character prediction model is constructed, early-stage accurate screening of filial generations is achieved through correlation analysis and marker verification, the error selection rate is reduced, and the breeding period is shortened.

The present application relates to the technical field of corn whole genome selection breeding, and discloses a data fusion analysis method and system for corn whole genome selection breeding, which comprises the following steps: collecting multi-dimensional data and constructing a multi-source data fusion network; generating a dynamic breeding data set through noise filtering and format unification processing; obtaining characteristic correlation data through spatiotemporal correlation processing; establishing a data-heredity response relationship model and generating heredity correlation dynamic data; generating core selection range data through selection efficiency correlation processing; constructing a statistical prediction model and optimizing breeding parameters. The system comprises data acquisition, data processing, correlation modeling, efficiency evaluation and selection analysis modules. The present application realizes efficient integration and dynamic analysis of multi-source data, improves the accuracy and efficiency of breeding selection, and is suitable for corn breeding optimization under complex environment.

The application discloses a method for creating a DH line of eggplant by using anther culture and application thereof, and relates to the technical field of plant breeding. The application establishes a morphological index of a flower bud based on a calyx lobe separation state, replaces a time-consuming microscope examination procedure, and optimizes a combination of heat treatment and low-temperature pretreatment, so that the embryoid induction rate is significantly improved. Finally, through strict ploidy identification and self-pollination purification, a DH line with consistent traits and stable heredity can be obtained, an efficient pure line source is provided for eggplant hybrid breeding, and efficient conversion from a heterozygous genotype plant to a homozygous DH line is realized.

The invention discloses a method for constructing and maintaining a nilaparvata lugens harmful experimental population, and belongs to the technical field of agricultural biology. According to the method, a directional feeding system based on host selection pressure is established, corresponding obligate identification varieties are selected as hosts according to different pest-causing types, and the specific pest-causing characteristics of the hosts are maintained by utilizing the host selection pressure; meanwhile, a regular monitoring system is established, and harmful drift of the population is found and corrected in time through a quality monitoring mechanism that purity spot check is performed every 5 generations and comprehensive evaluation is performed every 20 generations. Through the steps, the standard experimental population with clear damage type and stable heredity of the brown planthopper is constructed and maintained, the problems of inaccurate damage type identification and difficult population purity maintenance in the prior art are solved, and the method is suitable for screening of insect-resistant rice varieties, damage type monitoring and related basic research.

The invention discloses an identification method of a sequence participating in potato centromere relocation, and belongs to the technical field of plant heredity and molecular biology. According to the method, T2T-level genome data and CENH3 ChIP-seq data of different haplotypes of potatoes are integrated, sequence screening, annotation and function prediction are carried out by utilizing a bioinformatics analysis tool, and a satellite DNA sequence which is 2-3Kbp in length, is highly conserved in the same haplotype and can be specifically combined with CENH3 protein is identified. The sequence can induce CENH3 to deposit in a non-native centromere area, so that functional relocation of centromere is realized, and a core molecular element and a technical support are provided for artificial centromere construction, plant chromosome engineering and accurate genome operation. The method solves the problems that in the prior art, a functional sequence for clearly inducing centromere relocation is lacked, a CENH3 targeted recruitment mechanism model is not established and the like, and has a wide application prospect.

The invention provides a skin organoid HPV infection model based on a human embryonic stem cell source and a construction method and application thereof, and relates to the technical field of molecular biology, the construction method comprises the following steps: integrating an HPV genome to an AAVS1 site of genome DNA of a human embryonic stem cell to obtain the human embryonic stem cell carrying the HPV genome, and performing induced differentiation and activation of the HPV genome to obtain the human embryonic stem cell carrying the HPV genome. A skin-like organ HPV infection model is formed, stable heredity and controllable expression of an HPV genome are achieved, virus particle assembly is achieved through induced differentiation, establishment of a virus replication environment, activation of HPV early gene expression, driving of HPV genome replication and induction of late gene expression, and the natural infection state of HPV is simulated. The technical problem that in the prior art, an infection model cannot simulate the life cycle of HPV dependent terminal differentiation is solved, and an irreplaceable technical platform is provided for research of an HPV pathogenic mechanism and research and development of antiviral drugs and vaccines.

The invention discloses a chrysanthemum salt tolerance high-throughput identification method which comprises the following steps: selecting a plurality of chrysanthemum germplasm resources, setting sodium chloride solutions with different concentrations to carry out salt stress treatment, and carrying out dimensionality reduction calculation on a plurality of salt tolerance related characters through a principal component analysis method to obtain a comprehensive salt tolerance D value; after the salt stress phenotype appears, RGB images of each plant at four side view angles and one overlook angle are collected, and image color calibration and standardized naming are carried out; segmenting the image in an excessive green and HSV color space to obtain a plant binary image, extracting morphological and textural digital characteristic parameters, and analyzing the reliability of the characteristic parameters by using a linear regression model; adopting independent sample t test, variability analysis, feature importance analysis and generalized heritability analysis to screen representative features which are significant in salt stress response and stable in heredity from the digital feature parameters; the screened representative features are divided into a plurality of feature subsets, and a random forest algorithm is adopted to train a salt tolerance prediction model with the representative features as independent variables and salt tolerance D values as dependent variables; on the basis of a salt tolerance prediction model, evaluating the importance of each feature, screening out an optimal feature combination with the minimum prediction error, and performing salt tolerance grade automatic division on the chrysanthemum germplasm resources; according to the invention, lossless dynamic measurement of plants can be realized.

The invention belongs to the field of microorganisms, and particularly relates to monascus purpureus with high yield of essential amino acids as well as a screening method and application thereof. The invention provides a novel trans-membrane differentiation ARTP mutagenesis method aiming at filamentous fungi, aiming at the characteristics of multiple cores and thick walls of monascus, protoplast pretreatment and repair liquid components before mutagenesis are optimized, so that the fatality rate and the positive mutation rate are optimally balanced, and the problem of high instability of monascus heredity caused by conventional ARTP is solved. Based on the novel mutagenesis method, the mutant strain AZA5 is obtained through successful mutagenesis screening. The essential amino acid content of AZA5 is as high as 58%. The invention not only provides an excellent strain resource for industrial production of high-quality microbial protein, but also has important scientific value and wide industrial application potential.

The invention belongs to the technical field of plant biotechnology and molecular breeding, and relates to a ginseng 40K SNP liquid phase breeding chip and application thereof, the chip comprises 41684 SNP sites which are positioned on 24 chromosomes of a ginseng T2T reference genome (v2024), and the ginseng 40K SNP liquid phase breeding chip has the advantages of uniform site distribution, good representativeness, strong specificity and high polymorphism. The application range of the chip covers genetic diversity analysis of ginseng germplasm resources, population structure analysis, heredity and evolution analysis, genetic relationship identification, whole genome association analysis, genome selection and other molecular breeding related researches, the chip is developed based on a high-throughput sequencing technology, the detection throughput is high, detection of nearly thousands of materials can be covered at the same time, and the application range is wide. The method provided by the invention is suitable for mainstream second-generation sequencing platforms such as Illu mina and MGI, is simple, convenient and rapid to operate, provides key technical support for genetic resource innovation, excellent gene mining and precise molecular breeding of the ginseng, assists in cultivation of high-quality, high-yield and stress-resistant new varieties of the ginseng, and improves the core competitiveness of the ginseng industry.

The invention belongs to the technical field of plant heredity and genetic engineering, and particularly relates to soybean RING type E3 ubiquitin ligase GmSSE1 and application thereof. According to the invention, the soybean RING type E3 ubiquitin ligase gene GmSSE1 is separated from soybean Williams 82 (Williams 82), and tests prove that the expression of the gene is induced by salt stress, and the salt tolerance of soybean can be negatively regulated. Besides, according to haplotype analysis and phenotype correlation research, the single plant grain weights of different haplotypes (GmSSE1Hap1, GmSSE1Hap2 and GmSSE1Hap3) of the GmSSE1 in a natural population are obviously different. The invention not only provides an important gene resource for clarification of a plant salt-tolerant molecular mechanism, but also provides an effective molecular target and technical support for crop salt-tolerant genetic improvement, and has important theoretical significance and application potential.

The invention is applicable to the technical field of molecular biology and biology, and provides application of a corn biological clock gene Zmtoc1b in improvement of plant drought tolerance, the nucleotide sequence of the corn biological clock gene Zmtoc1b is shown as SEQ ID NO: 1, and the amino acid sequence of a protein Zmtoc1b encoded by the corn biological clock gene Zmtoc1b is shown as SEQ ID NO: 2. The Zmtoc1b gene is cloned from a corn inbred line B73, Arabidopsis thaliana is transformed through an agrobacterium-mediated method, a T3-generation transgenic line with stable inheritance is obtained, drought stress treatment results show that the drought tolerance of the transgenic Arabidopsis thaliana in the seedling stage is obviously higher than that of a wild type, the relative water content and SOD activity of leaves are increased, and the yield of the transgenic Arabidopsis thaliana is increased. Therefore, it is proved that the corn biological clock gene Zmtoc1b can effectively improve the drought tolerance of plants, and a key gene and technical support are provided for improving the stress resistance of the plants.

The invention discloses application of a GmPHD6 protein and a related biological material thereof in regulating and controlling the oil content of plant seeds. According to the invention, a GmPHD6 gene is over-expressed in a soybean receptor Jack by using a transgenic technology, and a GmPHD6 over-expression strain with stable inheritance is identified and screened out. The detection on the oil content in the GmPHD6 overexpression strain and the soybean receptor Jack seeds shows that compared with the soybean receptor Jack, the oil content of the GmPHD6 overexpression strain seeds is obviously improved and reaches a remarkable level. It is found for the first time that the GmPHD6 protein can positively regulate the grease content of the soybean seeds, the grease content of the soybean seeds can be remarkably improved by improving the GmPHD6 gene expression quantity, and the GmPHD6 protein has important theoretical and practical significance in improving the grease content of crop seeds, especially improving the grease content of the soybean seeds and cultivating high-grease soybean varieties.

The invention relates to the technical field of aquatic product breeding and gene engineering, in particular to a rapid all-male red crucian carp creation method and application thereof.The rapid all-male red crucian carp creation method comprises the steps that a CRISPR / Cas9 gene editing technology is utilized, a Foxl3 gene of wild type red crucian carp is knocked out in a targeted mode through double gRNA loci, an F0-generation gene knockout mutant is obtained, and the double gRNA loci are located on a third exon of the Foxl3 gene; and hybridizing the female fish in the F0-generation gene knockout mutant with the wild-type red crucian carp male fish to obtain the F1-generation all-male red crucian carp with the genotype of Foxl3 + / -. According to the method, complete separation and stable inheritance of phenotypes can be realized in the F1 generation.

The invention provides engineering vibrio natriticus for producing farnesene by using ethanol and a preparation method of farnesene, and relates to the technical field of bioengineering. In the embodiment of the invention, traditional chassis organisms such as yeast which grow slowly are abandoned, and the vibrio natriticus of which the doubling time is less than 10 minutes is used as a chassis; and engineering vibrio natrieti capable of efficiently utilizing ethanol is obtained by combining directed evolution, so that the thallus proliferation and fermentation period is greatly shortened. The selected exogenous plasmids Kesling 2187 and pFZ71 carry a gene cluster with a definite function, and after the gene cluster is successfully introduced into the vibrio natriticus, engineering vibrio natriticus with stable inheritance is formed. MVA pathway key genes (atoB, tHMG1 and the like) carried by the exogenous plasmids and a farnesene terminal synthesis gene (AFS1) have a synergistic effect, so that a complete and efficient synthesis pathway from ethanol to acetyl coenzyme A to FPP to alpha-farnesene is formed, the metabolic flux loss is reduced, and the farnesene synthesis flux is improved.

The application discloses a Brassica napus BnaREM1.3 gene and application of a coded protein thereof in regulation of drought resistance of crops. Overexpression strains of the BnaREM1.3 gene are obtained, and it is found that the drought resistance of the overexpression strains is significantly improved compared with wild type. Meanwhile, mutant strains of the BnaREM1.3 gene are obtained by using CRISPR / Cas9 technology, and it is found that the drought resistance of the mutant strains is significantly reduced compared with the wild type. The above results show that the Brassica napus BnaREM1.3 gene can be used to create Brassica napus with different drought resistance and stable heredity, and the BnaREM1.3 gene provides important gene resources and germplasm resources for drought resistance breeding of the Brassica napus, and has a wide application prospect in molecular design breeding of the Brassica napus.

The invention discloses a genotype filling optimization method for spruce norvegensis and a reference panel, and belongs to the technical field of forest heredity. The method comprises the following steps: constructing a haplotype reference panel based on large-scale picea norvegensis re-sequencing data; acquiring low-density genotype data; carrying out genotype filling by using IMPUTE5 software and integrating a genetic map of the picea norvegensis; and carrying out quality control on a filling result, and reserving a high-quality SNP site with Rsqsoft greater than or equal to 0.8. According to the method, key parameters and optimal configuration influencing filling accuracy are evaluated and determined for the spruce norveyi system for the first time, and the technical bottleneck that the species are lack of a reliable filling process is solved. Through the method and the special reference panel provided by the invention, high-quality whole genome filling data can be stably and efficiently obtained, and key technical support is provided for genetic research and breeding practice of picea norvegensis.