551 results about "Heredity" patented technology

Provided in the invention is a task scheduling method based on heredity and ant colony in a cloud computing environment. The method comprises the following methods: S1, initializing population; S2, selecting individuals according to a wheel disc type selection strategy; S3, carrying out crossover operation on the individuals according to crossover probability and carrying out reversion mutation operation according to a mutation probability so as to generate a new colony; S4, updating the new generated colony; S5, determining whether a dynamic fusion condition is met; S6, initializing ant pheromone by using an optimal solution found by heredity; S7, calculating probabilities of moving to next nodes by all ants and moving all the ants to the next nodes according to the probabilities; S8, enabling M ants to travelling N resource nodes and carrying out pheromone updating on an optimal ant cycle; S9, carrying out pheromone updating on all paths; and S10, determining whether an ant end condition is met and outputting an optimal solution. According to the invention, respective advantages of a genetic algorithm and an ant colony algorithm are drawn and respective defects are overcome; and on the basis of dynamic fusion of the two algorithms, time and efficiency of exact solution solving are both considered.

Secure network transaction system obtains user-authorized genetic term or bioinformatic profile, and transacts online service according to genetically-based user medical or other risk determined therefrom. Insurance policy, promotional offer, or other service may dynamically address genetically-based condition. Bioinformatic data classifies user per personal mask which filters subset of user genetic sequence. Risk profile may be calculated according to actuarial statistics, genetics and/or heredity using non-discriminatory rules specified for users in temporal or jurisdictional groups. User transactions are modifiable according to bioinformatic data representing genetically-based risk increase or decrease. Data is securely processed, modulated, and stored by network server for remote access and transaction using various portable user devices.

The invention discloses a global optimization, search and machine learning method based on the Lamarckian principle of inheritance of acquired characteristics, comprising step 1: constructing an objective function ƒ(P) according to the problem being solved, where P represents a set of candidate solutions to the problem; step 2: encoding P into a genetic algorithm (GA) chromosome, inputting or automatically calculating algorithmic parameters of the GA, and initializing the algorithm and the population of candidate solution generation G₀={P₀¹, P₀², . . . , P₀^S}, where S is the size of the population G and 0 stands for the initial generation; step 3: at generation k, optimizing the prevailing population of the candidate solutions G_k={P_k¹, P_k², . . . , P_k^S} iteratively using a Lamarckian “Heredity Operator” and a “Use-and-Disuse Operator” based on the values of ƒ(G_k); and step 4: outputting the final set of optimal solutions to the problem.

The invention relates to a seedling stage identification method for the resistance of tobacco to the bacterial wilt. The seedling stage identification method comprises the steps of heat preservation pool construction, pool water heating and circulation, wilt bacterium inoculation, tobacco seedling, disease state investigation and grading. The method replaces a conventional field identification result with a seedling stage identification result, identifies a great amount of material in a smaller area, carries out the identification a plurality of times annually, improves the reliability of the result, shortens the identification cycle, saves the site and test cost, and covers little floor space. The seedling stage identification method for the resistance of tobacco to the bacterial wilt is characterized by rapid disease development, good result repeatability, and the like. The seedling stage identification method for the resistance of tobacco to the bacterial wilt can effectively remove the interference on the resistance result caused by soil temperature difference, strain pathogenicity difference, strain quantity difference and other diseases, thereby benefiting the recurrence and comparison of results from different years and different labs. The difference between the attack rates of infection resistant varieties and the disease indexes is obvious; and the appraisal result of the resistance of the variety is basically consistent with the perennial resistance appraisal result obtained by others. The method has greater application potential in the resistance identification and resistance heredity rule study of disease resistant resources and breeding medium materials.

Described is a cyber security system for digital artifact genetic modeling and forensic analysis. The system identifies the provenance (origin) of a digital artifact by first receiving a plurality of digital artifacts, each digital artifact possessing features. Raw features are extracted from the digital artifacts. The raw features are classified into descriptive genotype-phonotype structures. Finally, lineage, heredity, and provenance of the digital artifacts are determined based on mapping of the genotype-phenotype structures.

A method for culturing the new variety of reed-catkins chicken includes such steps as choosing mutant cock and hen from local reed-catkins chicken, self-reproducing or transverse crossing, Choosing the cock and hen with transverse spots and light reed-catkins color, lateral crossing, choosing homozygotes, selective culturing, and repeating the previous two steps until stable heredity.

The invention discloses construction of a chronic disease risk assessment hyperbolic model and a disease predication system applying the chronic disease risk assessment hyperbolic model. A Shandong multi-center health management longitudinal observation queue is constructed according to longitudinal health management data of more than 20 health management centers in Shandong province to study the actions of heredity, environment, individual life style, health intervention factors and the like on the processes of outbreak, development and lapse of a major chronic disease, the risk assessment hyperbolic model suitable for various chronic diseases for health examination people of Shandong province and the disease predication system are established, and the scientific evidences are provided for the health intervention of the chronic diseases.

The invention relates to a slaughter type high-quality chilling fresh chicken breeding method. The breeding method includes: selecting recessive white-feather chickens (B line) having green shins and yellow skins as first male parents, and selecting large cockscomb high yield chickens (C line) having yellow shins and yellow skins as first female parents; hybridizing the B line and the C line to acquire parental generations (BC system) to be as final female parents according to the heredity principle that a green shin gene is sex-linked and an autosome controls a skin color gene, wherein parental chickens can be distinguished through the shin color, the shins of the female chickens are green, and the shins of the male chickens are yellow. using yellow-feather fast-growing chickens (A line) having green shins, yellow skins, and large cockscombs as final male parents, producing commodity chickens (ABC) by A line male X (B line male X C line female) female mating hybrid, wherein commodity male and female chickens have green shins, yellow skins, large cockscombs, and wide chests. The commodity chickens can be sold at the age of 63-65 days, the average weight of the female chickens is 1.6-1.65 Kg, the average weight of the male chickens is 1.8-1.85 Kg, the average uniformity can reach more than 96%; the dressing percentage, the whole net carcass rate, and the chest muscle rate can exceed 85%, 65%, and 18%, and the carcass character has significant advantages.

The invention discloses an engineered Saccharomyces cerevisiae producing heat-stability recombinant trypsin, and its application, and belongs to the genetic engineering field. A trypsin gene obtained through in-vitro amplification is fused with a leading short peptide YVEF, and is wholly connected to a Pichia pastoris GS115 chromosome to construct engineered Saccharomyces cerevisiae efficiently secreting and expressing recombinant trypsin, and the recombinant trypsin having an improved stability is obtained after purification, and the heat stabilities of the recombinant trypsin at 40DEG C, 50DEG C and 60DEG C are 1.77, 2.6 and 31 times wild trypsin respectively, so the problem of the low stability of trypsin is solved. The production of trypsin through applying the engineered Saccharomyces cerevisiae has the advantages of high output, simple technology, intelligible heredity and application background of engineered Saccharomyces cerevisiae, and convenient industrial application.

The invention provides a preparation method of glyphosate resistant transgenic rice. The method comprises the following steps: converting an expression vector containing glyphosate resistant gene into Agrobacterium tumefaciens, invading rice calluses by the Agrobacterium tumefaciens, transferring the calluses to a selective aluminum containing glyphosate for screening, choosing resistant calluses, differentiating, rooting, hardening-seedling, and transplanting to obtain glyphosate resistant transgenic rice. A molecular identification result shows that an exogenous gene is stably expressed. The obtained glyphosate resistant transgenic rice contains glyphosate herbicide resistant gene, contains no other screening marker genes, and can be used as a commercial herbicide resistant rice kind to reduce the reduce the later process and accelerate the breeding pace. The method has the advantages of simple operation, low copy number of transgenic plants, stable heredity properties, and good market application prospect.

The invention relates to the field of biotechnology, in particular to a virus seed for producing vaccines for preventing human rabies by utilizing human diploid cells (KMB17) and a preparation method thereof. In the invention, a rabies fixed virus CTN-1V5 strain is continuously subcultured in the human diploid cells (KMB17), and a terminal dilution method is used for screening viruses with highertiter, thereby obtaining a rabies virus strain which is suitable for the human diploid cells (KMB17) and has good immunogenicity and heredity stability, and culturing a rabies vaccine virus seed (CTN-DK strain) capable of efficiently reproducing in the human diploid cells (KMB17). By using the virus seed for producing a human diploid cell (KMB17) rabies vaccine, the risk caused by residual heterogonous DNA (deoxyribonucleic acid) in the vaccine which is currently used at home can be effectively avoided, and the safety and practicability of the rabies vaccine in China are further improved, thus the invention has great social and economic benefits.

The invention discloses an excised quick screening method for the salt-resistant strains of a China rose, which belongs to the field of agriculture biotechnology. The invention consists of 13 steps of the selecting and sterilizing of an initial explant, sprout inducing, propagation culture, the continuous subculture of propagated seedlings, the direct regeneration of vanes, the indirection regeneration of vanes, physical mutagenesis, mutagenesis resuming and culturing, the excised quick screening of the salt-resistant strains, the quick propagation and re-screening of the salt-resistant strains, resuming and culturing and seedling strengthening, rootage and transplanting. In the invention, the callus or the directly regenerated adventitious bud of the vane of the China rose is used as a processing material to mainly research the four key procedures of the excised screening technology, the subculture propagation, the seedling strengthening and the rootage culturing of the salt-resistant strains; the method has good repetitiveness, can effectively eliminate false positive strains, and obtain the salt-resistant strains with stable heredity; and the average total surviving rate of the salt-resistant screening thereof achieves about 18 percent which is improved by about 16 percent than comparison 1 and is improved about 18 percent than comparison 2; and the salt-resistant screening efficiency is greatly improved.

Methods for in vitro isolation and culture and induced differentiation of bovine muscle satellite cells relate to the methods for in vitro isolation and culture and induced differentiation of cells. The invention solves the following problems: the cost is high and the heredity stability is not good in the existing method for isolation and culture of bovine muscle satellite cells, and the obtained myotubes are fewer and do not have contracting function in the existing method for induced differentiation of bovine muscle satellite cells. The method for in vitro isolation and culture of bovine muscle satellite cells is characterized by cleaning the tissues and digesting the tissues with trypsin and collagenase solution; carrying out centrifuging after filtering, re-suspending the cells and culturing the cells by a differential velocity adherent method after inoculation; and culturing the cells until the merging rate is 95% and carrying out heredity with pancreatin. The method for induced differentiation of bovine muscle satellite cells is characterized by obtaining PEI-plasmid complexes; culturing the bovine muscle satellite cells until the merging rate is 75%, adding high-glucose DMEM culture solution after cleaning, adding the PEI-plasmid complexes to the surfaces of the cells, changing culture solution A for culturing and then changing culture solution B for induced differentiation. In the method, the cost for isolation and culture of the bovine muscle satellite cells is low, the heredity stability is good and the myotubes obtained through induced differentiation are large in quantity and have contracting function.

The invention discloses a BRCA gene susceptibility SNP locus detection composition. The BRCA gene susceptibility SNP locus detection composition comprises a primer of SEQ NO:1-SEQ NO:448. The BRCA gene susceptibility SNP locus detection composition has the advantages that more than two thousand susceptibility SNP loci of a BRCA gene can be detected at one time, for the susceptibility SNP loci relative to breast cancer and ovarian cancer, the detection throughput is high, the specificity is strong, the pollution is not prone to occur, and the safety is high, the detection result has good accuracy and repeatability, and certain auxiliary diagnosis and implication effects are achieved for susceptible population of the breast cancer and the ovarian cancer, especially for crowd with family heredity of the two cancers.

The invention discloses a breeding method for amphiprotic fertile autotetraploid crucian carps and an establishment method for strains of the crucian carps. The breeding method includes the following steps that allotetraploid crucian carps are selected from crucian carp hybridization F1 offspring with red crucian carps as female parents and megalobrama amblycephala as male parents, female individuals capable of generating ova of different sizes and male individuals capable of generating watery semen are selected from the allotetraploid crucian carps for hasten parturition, insemination is carried out manually through a dry method, running water incubation is conducted on fertilized ova, then the fertilized ova are bred, and the amphiprotic fertile autotetraploid crucian carps are selected from selfing offspring. Selfing is carried out on the male individuals and the female individuals in the obtained autotetraploid crucian carps, and the strains of the amphiprotic fertile autotetraploid crucian carps can be established and obtained through multi-offspring breeding. Ploid hybridization is conducted on the autotetraploid crucian carp colonies obtained through the breeding method and diploid crucian carps, so allotriploid fishes can be bred on a large scale. The strains of the crucian carps obtained through the breeding method are excellent in character, stable in heredity and high in fertilization rate and hatchability, and the breeding method has a great significance in genetic research of fishes.

The invention provides a type O foot-and-mouth disease virus mutant and a preparation method and application thereof, and belongs to the technical field of vaccine candidate strains. According to thetype O foot-and-mouth disease virus mutant disclosed by the invention, an rHN virus strain is used as a female parent virus strain, and the following amino acids in a G-H ring of VP3 protein are mutated: the 173rd aspartic acid is mutated into asparagine, the 174th valine is mutated into glutamic acid and the 179th asparagine is mutated into cysteine. The virus mutant has heredity stability and obtains the capacity of caveolin for performing mediated infestation on CHO-K1cells. The result of detecting the cross neutralization capacity of immune positive serum indicates that compared with a female parent virus strain, the virus mutant has the advantages that the cross protection capacity of the virus mutant for inducing organisms to produce foot-and-mouth disease virus neutralizing antibodies is notably improved, and the virus mutant shows excellent antigen broad spectrum properties. Vaccines prepared through inactivation of the virus mutant can be used for preventing infection with type O foot-and-mouth disease viruses.

A network flow filtering system and method based on a field programmable gate array (FPGA) belongs to the field of network application. The method includes first utilizing a heredity K-means algorithm to conduct off-line practice on network historical flow to generate a flow classification knowledge base, utilizing the heredity K-means algorithm to recognize and distinguish flow classes online by combining the flow classification knowledge base and determining whether the flow is required to be filtered according to a recognition result. The recognition method based on the heredity K-means solves the problem of low accuracy in recognition of novel application protocol flow of a traditional method. The online recognition and the filtering process are achieved based on the FPGA technology. An online recognition and filtering internet protocol (IP) core operating in the FPGA can process network data packages in real time, extracts characteristic attributes of each flow, calculates and recognizes the flow class in real time and filters the flow in real time according to the recognition result. The system and method has good real-time performance and cannot obviously affect effective bandwidth of the network in the implementation process.

The invention discloses a continuous recessive character backcrossing technology which is characterized by taking a material which contains no target recessive genes and is ready to be improved as a recurrent parent, and taking a material which contains the target recessive genes as a donor parent; taking the recurrent parent as the parent to be hybridized with the donor parent and harvesting hybrid seeds of the first generation F1 and recurrent parent seeds; and planting the hybrid seeds of the first generation F1 and the recurrent parent seeds till the planting of a group containing backcross hybrid seeds of the second of n generations BCn F2 and till the typing into a target recessive gene isogenic line of the recurrent parent. The invention also discloses a method for creating a purpleleaf marker isogenic line by applying the continuous recessive character backcrossing technology and also a method for transferring new purple leaf marker nucleus sterile line by taking the purple leaf marker isogenic line as an intermediate material. In the methods, a selfing process is combined with a next backcrossing process for the treatment in one season, therefore, the time efficiency is improved by about 50 percent, a small number of dominant-gene-controlled unsuitable characters of existing fine-quality parents can be fast improved, and useful recessive characters can be fast imported to the present high-level heredity background.

The invention discloses a Xundian county wheat summer planting generation-adding method which belongs to the technical field of wheat summer planting generation adding. Optimal dormancy breaking methods, vernalization conditions, seedtime and field management modes of different winter and spring wheats are researched, thereby a complete set of wheat summer planting technical system is established, theoretical and technical supports are provided for summer planting generation adding of nationwide wheat heredity and breeding and Yunnan province wheat parent and elite seed expanding propagation, the improvement of food security of China is facilitated, and the social harmony and stability and rural economic development are promoted. By means of the method, the wheat summer planting generation adding can be well achieved in Xundian county.

The invention provides a method for screening cattle high altitude hypoxia adaptation specific molecular marker, and furthermore a cattle kind or individual which is suitable for high altitude hypoxiasurvival is screened through the specific marker. The method aims at a high altitude hypoxia adaptation heredity characteristic of a kettle kind, and selects local cattle kinds which are distributedin high altitude regions at aspects of genome evolution, selecting and adapting. Multiple genome selecting signals, a full-genome association analysis method and a strategy are combined. Key genes andmolecular makers which are suitable for high altitude hypoxia are efficiently and accurately screened. Reasonable method designed is realized. The detecting method according to the key gene and marker designing has advantages of high accuracy, and convenient application operation. According to the method of the invention, through analyzing different altitude cattle kinds, a gene ACSS2 which is related with high altitude hypoxia adaptation and a haplotype thereof are found; and furthermore a specific SNP which bears a strongest selecting signal is positioned; and the method realizes an important meaning and a high practicability value for cattle molecule breeding operation.

The invention belongs to the technical field of animal epidemic disease serological diagnosis, and relates to an enzyme-linked immunosorbent assay vector and a kit for detecting avian leukosis P27. The avian leukemia virus (P27) enzyme linked immunosorbent assay kit comprises a 96 hole elisa plate coating a avian leukosis P27 polyclonal antibody, P27 monoclonal antibody marked by alkaline phosphatase, a substrate solution, a stop solution and a cleaning solution. The vian leukosis P27 polyclonal antibody and the P27 monoclonal antibody marked by alkaline phosphatase effectively improve the sensitivity, the specificity and the stability of the detection. The invention provides an efficient and sensitive ELISA (enzyme linked immunosorbent assay) detection kit for sifting out and purifying avian leukosis positive chickens and cultivating new species with heredity resistance, and the kit is low in cost and simple and convenient to operate, and is applicable to promotion in animal husbandry.

The invention first provides a Cynoglossus semilaevis gender specific microsatellite marker, and comprises a microsatellite marker located in the Z chromosome, and the nucleotide sequence is SEQ ID NO:1. The invention also provides primers designed based on the above microsatellite marker, and the sequences of the upstream primer and the downstream primer are SEQ ID NO:2 and SEQ ID NO:3 respectively. The invention screens a Cynoglossus semilaevis gender specific microsatellite marker, and the Cynoglossus semilaevis gender specific microsatellite marker is subjected to SCAR conversion. Primers marked by SCAR are designed for Cynoglossus semilaevis heredity gender identification. Female, male and superfemale individuals of Cynoglossus semilaevis can be distinguished rapidly, accurately and effectively. Because the marker has characteristics that objective straps can be amplified in females and males and the objective straps can be distinguished with agarose electrophoresis, the time for accurate identification of Cynoglossus semilaevis heredity genders is shortened obviously, the on-site batch identification of Cynoglossus semilaevis heredity genders in a culturing farm of Cynoglossus semilaevis can be carried out, the detection cost is saved, and the work efficiency and accuracy of on-site detection of Cynoglossus semilaevis heredity genders in a culturing farm are raised.

The invention discloses a detection method suitable for molecular identification and authentic right identification of a crop variety. Multiple methods and technologies are comprehensively utilized, marker sits undergo omnibearing multi-angle assessment, and a suitable core and expansion site combination is determined to protrude the detection efficiency on the premise of meeting the detection accuracy. The method comprises the following steps: 1, preparing dozens of to hundreds of core site combinations having the characteristics of high stability, high variety distinguishing ability and good compatibility with multiple platforms and being able to highly-efficiently distinguish different varieties; 2, preparing thousands of to tens of thousands of expansion site combinations having the characteristics of uniform distribution and good compatibility with a chip platform and being able to accurately evaluate the heredity similarity among the varieties; and 3, using the two groups of the sites: primarily using the core sites to carry out detection during the identification of the varieties, and adopting the expansion sites to continuously carry out detection if a sample to be detected and a contrast sample cannot be effectively distinguished. The detection method is suitable for the standard DNA fingerprint data construction, molecular identification and authentic right identification of the crop variety.

The invention discloses an automatic k adjacent local search heredity clustering method for a graphic image, which mainly overcomes the defect of the conventional automatic clustering algorithm that the local optimization is easy to cause. The automatic k adjacent local search heredity clustering method comprises the realization steps of: (1) detecting an outline of an image by utilizing a canny edge detector; (2) describing the outline of the image by utilizing a shape context method and calculating a matched cost matrix of an outline point; (3) matching the outline point by utilizing a dynamic programming method according to the matched cost matrix; (4) converting the matched outline point by utilizing a procrustes analysis method; (5) representing the converted matched outline point and measuring an edit distance between character strings; (6) calculating the distance between the images according to the edit distance of the character strings; (7) clustering the images by utilizing a heredity automatic clustering method; and (8) carrying out k adjacent local search on groups of a heredity method. The automatic k adjacent local search heredity clustering method for the graphic image has the advantages that overall optimization is easy to achieve and an accurate clustering number can be found out.

The invention relates to a molecular marker for identifying gemale plant and male plant of gingo, more particularl, the invention relates to a method for identifying gender of gingo. Compared to the usage of random primer, the method has increased stability, and compared to the dominant marker, which can provide limit information, the method is improved. According to the mehtod, the gingo male plant specificity SCAR-GBM primer and the exsisted atp1 primer of the mitochondrial DNA which is developed in the field are used at the same time for performing multiple PCR, wherein, the SCAR-GBM primer is based on the sequence development of RAPD segment which can display the heredity of gingo male plant.