143 results about "Genetic diversity" patented technology

The invention discloses a cowpea 5K SNP (Single Nucleotide Polymorphism) liquid chip and application thereof in variety identification, and belongs to the technical field of biology. The liquid chip provided by the invention comprises a set of nucleotide probes for detecting 5847 SNP sites of cowpea, and can be widely applied to different scenes of germplasm resource identification, genetic diversity evaluation, variety identification, genetic relationship identification, purification and rejuvenation of local varieties and the like of different cowpea varieties; an effective scientific basis is provided for cowpea variety identification, the variety identification and evaluation efficiency is improved, and the method has a remarkable application value.

The invention belongs to the technical field of molecular biology and plant molecular breeding, and particularly relates to a mustard core SNP molecular marker set and a screening method and application thereof. The invention provides a core SNP marker set (33) which is subjected to whole genome re-sequencing screening and experimental verification and is suitable for mustard germplasm resource identification and genetic analysis for the first time. The marker set is high in polymorphism and good in stability, covers the whole genome and can effectively distinguish different leaf mustard germplasms. By utilizing the core marker set and the matched KASP primer, the genetic typing of the leaf mustard germplasm resources can be quickly and accurately performed with high throughput, and the defects that the traditional morphological identification is time-consuming, labor-consuming and poor in accuracy are overcome. The marker set can be used for analyzing the genetic diversity, the population structure and the phylogenetic relationship of the leaf mustard germplasm resources, and a molecular basis is provided for collection, preservation and evaluation of the germplasm resources and breeding parent matching.

The invention discloses a compression and decompression method based on generic genome expression, and relates to the technical field of compression and decompression of DNA next-generation sequencing data, in particular to the compression and decompression method based on generic genome expression. The method aims at solving the problems that in the prior art, the capacity of processing population genetic diversity is insufficient, original sequencing quality information cannot be effectively restored during decompression, and memory occupation is too high during large-scale data processing. Obtaining a to-be-compressed sequencing sequence data file, a reference genome sequence and a thousand-person genome variation sample; obtaining a haplotype list, a variation list and a haplotype offset list corresponding to each window block; storing the window number, the haplotype number, the haplotype offset, the head and tail unmatched sequences, the current sequence name and the quality score character string into a single compression block; carrying out binding storage; completing the compression processing of the mass fraction; and obtaining each to-be-compressed sequencing sequence based on the result of the compressed part.

The invention belongs to the technical field of molecular biology, bioinformatics, sorghum genetics and whole-genome gene chips, and particularly relates to a sorghum whole-genome SNP molecular marker combination developed based on a targeted sequencing technology, a 10K liquid chip and application of the sorghum whole-genome SNP molecular marker combination and the 10K liquid chip. The SNP molecular marker combination comprises 10,000 SNP loci, and the specific information of the 10,000 SNP loci is as shown in a table 1; the physical positions of the 10000 SNP loci are determined by performing sequence alignment based on a reference genome of the wine red tassel sorghum variety HYZ-T2T with the version number of v1.0. A liquid chip developed based on the SNP molecular marker combination provided by the invention can be effectively applied to genetic diversity analysis, whole genome association analysis, genetic typing, molecular marker-assisted selective breeding, whole genome selective breeding and other applications of sorghum germplasm resources.

The invention belongs to the field of microbial breeding and fermentation engineering, and particularly relates to a saccharomyces cerevisiae mutagenesis strain with high yield of S-adenosyl-L-methionine as well as a mutagenesis method and application of the saccharomyces cerevisiae mutagenesis strain. Saccharomyces cerevisiae is induced to generate genetic diversity through multiple rounds of ultraviolet mutagenesis, and high-throughput screening is performed in combination with strain color phenotypic difference; and the mutant strain HY2402-Z18 with high yield of S-adenosine-L-methionine and genetic stability is obtained. The SAM yield of the mutagenic strain is increased by 40.1% compared with that of an original strain, the SAM yield reaches 13.44 g / L when the mutagenic strain is fermented for 60 h in a 5 L fermentation tank system, the unit yield reaches 0.156 g / L / OD, and the methionine conversion rate is 75%. After thalli are collected through centrifugation of fermentation liquor, an S-adenosine-L-methionine product with the purity of 98%-100% can be obtained through purification of ion exchange resin, no by-products are accumulated, the yield, the substrate conversion rate and the production efficiency of the S-adenosine-L-methionine can be remarkably improved when the mutagenesis strain is used for fermentation production, and the production cost is reduced. The production cost is effectively reduced, the product purity and quality are improved, and the method is suitable for industrial large-scale production of SAM.

This invention relates to the fields of genomics, molecular biology, bioinformatics, and genome-wide selection breeding, specifically a 20k liquid-phase breeding chip for the whole genome of *S. esculenta* and its applications. The liquid-phase chip contains background SNPs and functional SNPs located on the *S. esculenta* reference genome; wherein the background SNPs are uniformly distributed within the genome; and the functional SNPs are associated with important economic traits of *S. esculenta*; these important economic traits include one or more of the following: saponin content, polysaccharide content, and heat tolerance. The chip can be applied to the assessment of genetic diversity in *S. esculenta*, identification of germplasm resources and phylogenetic relationships, genome-wide association analysis of important economic traits, and genome-wide selection breeding. This chip has advantages such as high throughput, high region coverage, high locus detection rate, and high flexibility, providing powerful tool support for molecular breeding of *S. esculenta*.

The invention discloses a corn genome breeding 45K chip and application thereof, the chip covers 44, 637 SNP loci and 27 InDel loci, and the SNP loci are uniformly distributed on 10 chromosomes and part of scaffold of a corn genome and have rich polymorphism. Compared with an existing corn chip, the SNP marker of the 45K chip is derived from re-sequencing results of 507 corn varieties, has better variety representativeness, and contains 925 key functional gene loci for close planting and high yield and 96 corn variety authenticity identification loci; the method can be more effectively used for functional gene identification, genetic diversity analysis, heterosis group division, whole genome selection analysis and variety authenticity identification.

The invention belongs to the technical field of biology, and particularly relates to a sophora tonkinensis SSR molecular marker primer group, a kit as well as application and a method of the sophora tonkinensis SSR molecular marker primer group. The sophora tonkinensis SSR molecular marker primer group comprises 15 pairs of primers, the nucleotide sequences of the 15 pairs of primers are sequentially shown as SEQ ID NO.1-SEQ ID NO.30, and the upstream primer in each primer pair is marked with a fluorophore. The sophora tonkinensis SSR molecular marker primer provided by the invention is high in polymorphism, and can effectively reveal the genetic diversity of sophora tonkinensis; a fingerprint database of sophora tonkinensis germplasm is also constructed, and the variety of sophora tonkinensis to be detected can be identified through comparison; the number of the SSR molecular markers of the sophora tonkinensis is increased, a tool is provided for genetic diversity research and germplasm resource analysis of the sophora tonkinensis, and a theoretical basis is provided for protection and utilization of sophora tonkinensis resources.

The invention relates to the technical field of plant molecular markers, in particular to a millet whole genome 65K liquid chip and application thereof.The millet whole genome 65K liquid chip is obtained through the following steps that re-sequencing data of a millet material is obtained, and then the re-sequencing data is subjected to quality control, mapping with a reference genome and identification and screening of variation sites; according to the indexes of the variation sites, combining the positions of the sites on the chromosomes, analyzing upstream and downstream sequences of the variation sites, and selecting the variation sites which can be used for chip development; the obtained variation site and 50bp sequences before and after the variation site are used, and an Infinium chip manufacturing technology is used for manufacturing the millet whole genome 65K liquid phase chip; based on high-depth whole genome re-sequencing data of large-scale millet germplasm materials, the millet whole genome 65K liquid chip is successfully developed, and can be widely applied to the fields of millet genotype detection, variety identification, genetic positioning, genetic diversity analysis, whole genome correlation analysis, whole genome selective breeding and the like. And important technical support is provided for molecular breeding development of millet.

The invention discloses a blocky coral breeding and transplanting combined device and method, and relates to the technical field of artificial coral reefs, and the blocky coral breeding and transplanting combined device comprises a base support with a through hole and a bearing surface; the fixing piece is provided with a plurality of mounting holes and connecting holes; the block-shaped coral is provided with a round hole; the connecting piece is provided with a protruding part, and the protruding part penetrates through the round hole to fix or press the block-shaped coral on the base support and extends to the connecting hole to fix the base support on one face or two faces of the fixing piece. Modular design is adopted, all parts can be flexibly combined so as to improve the operation efficiency, and large-scale carrying, transportation and production are facilitated. The operation efficiency of submarine divers can be effectively improved, and meanwhile the survival rate of coral transplantation and transportation in different places is increased. In addition, the problem that blocky coral varieties cannot be transplanted through a traditional transplanting method is solved, and the genetic diversity of coral repair is greatly improved.

The invention discloses an SNP (Single Nucleotide Polymorphism) marker related to a golden red paramisgurnus dabryanus body color character and application thereof, and relates to the technical field of molecular markers. The SNP marker is located at the 1354bp position of an rp2 gene of a No.7 chromosome of paramisgurnus dabryanus, the polymorphic form of the SNP marker is A / G, an individual of an AA genotype has a golden red body color, and individuals of AG and GG genotypes have a wild body color. The invention further provides a primer pair and a kit for detecting the SNP marker and application of the primer pair and the kit in detecting the body color character of the golden red paramisgurnus dabryanus and breeding. The nucleotide sequence of the primer pair is shown as SEQ ID NO: 2-SEQ ID NO: 3. The molecular marker provided by the invention can be used for detecting the body color of the paramisgurnus dabryanus and breeding the body color character of the golden red paramisgurnus dabryanus, so that the genetic diversity of population offspring is effectively increased, and the breeding process of breeding of a new variety of the body color of the red paramisgurnus dabryanus is remarkably promoted.

The present application relates to the technical field of molecular marker development and molecular detection, discloses development of dwarf tomato InDel molecular marker primer, provides a group of dwarf tomato InDel molecular markers, the dwarf tomato InDel molecular marker includes 79 InDel markers shown in the following table, and the dwarf tomato InDel molecular marker is determined with reference to genome SL5.0.Using the dwarf tomato InDel molecular marker can provide technical support for carrying out target DNA sample rapid molecular detection in dwarf tomato;Since the set of markers is distributed on multiple chromosomes of the dwarf tomato genome, can be selected and used according to actual needs, the genomic DNA of the candidate material can be detected at any stage, has the advantages of flexible use, simple operation, high detection efficiency, less limiting factors, stable and accurate detection results, provides a simple, flexible and efficient way for genetic linkage map construction, genetic diversity analysis, resource identification, hybrid / purity identification and the like in the dwarf tomato.

The invention discloses a probe for detecting a penaeus japonicus SNP molecular marker combination, a penaeus japonicus 31K liquid phase breeding chip and application, and belongs to the technical field of molecular marker breeding. The penaeus japonicus SNP molecular marker combination is composed of 31097 SNP molecular markers. The 31097 SNP molecular markers are as shown in the specification table 1. The invention also provides application of the penaeus japonicus liquid phase breeding chip in penaeus japonicus breeding, sample detection, genetic resource genetic diversity analysis, genetic and evolutionary analysis, genetic relationship identification, whole genome association analysis and penaeus japonicus genome selection. The penaeus japonicus 31K liquid phase breeding chip provided by the invention has the characteristics of good site representativeness, strong specificity, high polymorphism and the like, is widely applicable to detection of different varieties of penaeus japonicus materials, can establish a modern breeding system, and promotes the development of the penaeus japonicus molecular breeding industry.

The invention provides an assembled and annotated tilia amurensis mitochondrial genome, and relates to the fields of molecular biology and plant genetics. The tilia amurensis mitochondrial genome is a single cyclic molecule; the total length of the tilia amurensis mitochondrial genome is 830088bp; the GC content of the tilia amurensis mitochondrial genome is 44.97%, the complete sequence of the tilia amurensis mitochondrial genome is reported for the first time, high repeatability and chloroplast sequence migration characteristics of the tilia amurensis mitochondrial genome are disclosed, and a molecular foundation is laid for endangered mechanism analysis, genetic diversity evaluation and protection strategy formulation of tilia amurensis.

This invention discloses an SSR molecular marker primer set for identifying Amomum villosum varieties, along with its identification method and applications. The SSR molecular marker primer set provided by this invention contains 27 primer pairs and possesses advantages such as high polymorphism, stable results, good reproducibility, and simple operation. Using this primer set for amplification and detection can be used to identify different Amomum villosum varieties, construct a DNA fingerprint information database of Amomum villosum germplasm, and compile a statistical database of genetic diversity information. This invention also provides a method for identifying Amomum villosum varieties based on SSR molecular markers. By amplifying Amomum villosum samples using the SSR molecular marker primer set and performing statistical analysis based on the number of differential loci, different Amomum villosum varieties can be quickly and accurately distinguished and identified, clarifying the genetic background of Amomum villosum germplasm, guiding scientific breeding, and providing a more effective method for identifying Amomum villosum varieties. This method is of great significance for Amomum villosum breeding.

The application belongs to the technical field of molecular biology, and particularly relates to a macadamia SNP molecular marker combination and application thereof. The SNP molecular marker combination comprises 284 SNP sites, the 284 SNP sites can uniformly cover a macadamia genome, have high polymorphism information content, and can be used for constructing a macadamia DNA fingerprint map, and on the basis of the DNA fingerprint map, the SNP molecular marker combination can be further used for identifying macadamia germplasm resources, and can solve problems such as mixed germplasm in resources, repeated preservation and management, and intellectual property right disputes. Meanwhile, the 284 SNP sites are used for screening macadamia core germplasm, so that the most abundant genetic diversity of a species can be maximally represented by the least number of germplasm and the lowest genetic redundancy, the management and utilization efficiency of the germplasm resources is improved, the genetic resources are effectively protected and utilized, and useful genetic resources are selected for a macadamia breeding plan.

The invention provides a construction method of a Chongqing walnut germplasm DNA fingerprint spectrum based on an InDel marker. According to the invention, an InDel marker is combined with a whole genome re-sequencing technology, effective polymorphic markers of walnuts are obtained through screening, and an optimal core marker is selected through Chongqing walnut resources to construct a DNA fingerprint spectrum. The research result provides an efficient technical means for accurate identification and genetic diversity analysis of Chongqing walnut germplasm resources, breaks the current bottlenecks of chaos of germplasm management and insufficient excavation of excellent resources, can directly support local high-quality high-resistance walnut variety breeding, and assists quality improvement, efficiency improvement and healthy sustainable development of the Chongqing walnut industry; meanwhile, a key theoretical basis and a practical reference are provided for construction of a national walnut germplasm resource molecular identification technology system and fingerprint spectrum standardization research, and the technical blank in the walnut InDel marker application field is filled.

The invention belongs to the technical field of animal molecular biology and animal genetic breeding, and particularly relates to a buffalo whole genome 20k liquid-phase SNP chip and a design method and application thereof, and the chip comprises 24893 SNP molecular markers; according to the method, four selection signal analysis (FST, Piratio, XP-CLR and XP-EHH) are jointly applied to screen 11 buffalo group comparison combinations, it is ensured that the sites have high information content and group universality, through multiple rounds of quality control and function annotation, finally 24893 SNP sites are carefully selected, the whole genome is covered in a balanced mode, and the redundancy is remarkably reduced; based on 115 sample data of 11 main Chinese buffalo varieties (including marsh type and river type), the chip is ensured to be suitable for multi-variety buffalo groups, and the application advantages of variety identification, genetic analysis, genetic diversity monitoring and the like are highlighted.

The application belongs to the technical field of plant molecular markers, and particularly relates to a set of Chinese sheep fescue SSR molecular marker primers and application thereof. The Chinese sheep fescue SSR molecular marker primers provided by the application include 18 pairs of specific primers. The primers are used for PCR amplification and electrophoretic detection of genomic DNA of Chinese sheep fescue samples, so that Chinese sheep fescue germplasm resource identification, genetic diversity analysis and molecular marker assisted breeding can be realized. The application successfully reduces the detection cost by optimizing the reaction system, and also has the characteristics of high specificity and good stability, and has important application value for Chinese sheep fescue germplasm resource evaluation and molecular breeding.

The application relates to a SNP molecular marker combination for individual identification of Gallicrex cinerea and application thereof, and relates to the technical field of biology. The SNP molecular marker combination comprises 59 SNP molecular markers. Based on the SNP molecular marker combination, target SNP site information of the Gallicrex cinerea can be quickly and accurately obtained, so that individual identification of the Gallicrex cinerea is carried out, and a foundation is laid for individual identification and genetic diversity research of the Gallicrex cinerea.

This invention provides SSR molecular markers for Hibiscus rosa-sinus and their applications, belonging to the field of molecular marker technology. The invention comprises 15 molecular markers, with corresponding primer sequences shown in SEQ ID NO. 1~30. Testing revealed that this series of markers exhibits a 100% polymorphism rate, excellent polymorphism information content, marker index, and resolution, and strong genetic stability. The molecular marker primers of this invention can efficiently distinguish Hibiscus rosa-sinus germplasm materials, clearly revealing the population's genetic structure and phylogenetic relationships. They are suitable for Hibiscus rosa-sinus germplasm resource identification, genetic diversity analysis, molecular fingerprinting construction, and assisted breeding, possessing the characteristics of high specificity, high resolution, and wide applicability.

The invention discloses application of a diplotype of a high-association SNP (Single Nucleotide Polymorphism) site of a rudon septentrionalis body in breeding, and belongs to the technical field of aquatic breeding. The method comprises the following steps: analyzing early-stage sequencing data of a W group and an M group of navodon septentrionalis to identify two linkage imbalance SNP sites at 269 and 469 positions of an MYF6 gene with relatively high genetic diversity, totally detecting three haplotypes in the W group and the M group, and randomly forming five diplotypes by the three haplotypes, compared with other four diplotypes, the homozygous diplotype V (G269G269C469C46969) has remarkable advantages in the aspects of average body height and MYF6 gene expression, can be used as a molecular marker related to the body height, and is applied to molecule-assisted breeding of a new fast-growing strain of the navodon septentrionalis so as to improve the yield of a fast-growing improved variety of the navodon septentrionalis.

The invention relates to the technical field of algal moss germplasm resource preservation, in particular to a low-temperature preservation method of algal moss germplasm resources, which comprises the following steps: preparing an improved Benecke Takakia culture medium (containing NH4NO3, KH2PO4 and other components, the pH value is 5.8-6.0, and GellanGum and filter paper are added); cleaning an algae moss material with sterile water, disinfecting and detoxifying with ethanol and HgCl2, and inoculating the algae moss material to the culture medium; short-term preservation is carried out at 4 DEG C, and long-term preservation is carried out by placing the material at 4 DEG C for 48 hours and then transferring to-20 DEG C. According to the low-temperature preservation method for the algae moss germplasm resources, the survival rate of the algae moss after being preserved at 4 DEG C for 6 months and 18 months at-20 DEG C can reach 50% or above, the material preserved at-20 DEG C can normally grow and breed after being recovered, and the problems that pollution is likely to happen and the survival rate is low when normal-temperature preservation is carried out are solved; the method is of great significance to protection of algae moss and biological and gene diversity.

The invention relates to the technical field of agricultural biology, and discloses a plant feed genetic marker FTIR spectrum screening method which comprises the following steps: firstly, acquiring feed samples of a plurality of plant individuals from a plant population with genetic diversity, and collecting FTIR spectrum data of the feed samples; preprocessing the spectral data and extracting quantified spectral characteristic parameters, thereby establishing a spectral phenotype for each plant individual; acquiring genetic information of the plant individuals, wherein the genetic information comprises genetic locus data or gene expression profile data; and finally, carrying out correlation analysis on the established spectral phenotype and corresponding genetic information to identify one or more genetic markers significantly related to the spectral phenotype. Efficient nondestructive evaluation of the chemical quality of the plant feed is achieved through the FTIR spectrum technology, related genetic markers are accurately identified in combination with genetic information, and an effective way is provided for accelerating breeding of high-nutritive-value feed varieties and revealing a molecular mechanism of quality formation.

The invention discloses a domestic rabbit whole genome multifunctional chip and application, and belongs to the technical field of molecular biology. The invention relates to a rabbit whole genome 10K liquid phase breeding chip based on a target area genome sequence liquid phase capture accurate positioning sequencing typing technology. The chip comprises 10331 SNP (Single Nucleotide Polymorphism) loci. The chip can be applied to rabbit genetic diversity evaluation, germplasm resource and genetic relationship identification, important economic character whole genome association analysis and whole genome selective breeding, has the advantages of high throughput, high interval coverage rate, high locus detection rate, high flexibility and the like, and provides powerful tool support for rabbit molecular breeding and variety identification.

The invention provides a rice whole genome InDel molecular marker system based on capillary electrophoresis, the InDel molecular marker system comprises an InDel primer pair corresponding to 61 InDel molecular marker sites, and the nucleotide sequences of the InDel primer pair are as shown in SEQ ID NO. 1 to SEQ ID NO. 122. The invention also provides a preparation method of the rice whole genome InDel molecular marker system based on capillary electrophoresis. The invention further provides application of the rice whole genome InDel molecular marker system. The rice whole genome InDel molecular marker system can be applied to rice genetic map construction, rice molecular breeding and rice variety identification. Based on the high-precision characteristic of capillary gel electrophoresis (CGE) and the next-generation sequencing technology, a set of InDel molecular marker system covering 61 genome polymorphisms with the average difference fragment length of 8.4 bp of the whole rice genome is developed, and a new method is provided for the aspects of rice genetic diversity analysis, variety identification, map-based cloning and the like.

The invention relates to the technical field of gene chips, in particular to a sorghum whole genome liquid phase chip as well as a construction method and application thereof. A detection target of the sorghum whole genome liquid phase chip disclosed by the invention is composed of 5446 SNP molecular markers and 3 InDel molecular markers; the positions of the 5446 SNP molecular markers and the 3 InDel molecular markers, which are positioned on a sorghum reference genome Sorghumbicolv3.1. 1, are as shown in a table 1; the sorghum whole genome liquid chip can be used for sorghum variety identification, genetic relationship identification, genetic diversity analysis, molecular genetic map construction, gene positioning and cloning, molecular marker-assisted selection and whole genome selective breeding, and provides an important molecular basis for important agronomic trait analysis and biological breeding of sorghum.

The invention provides a primer pair combination of an SNP (Single Nucleotide Polymorphism) molecular marker combination for constructing a peony DNA (Deoxyribonucleic Acid) fingerprint spectrum. The DNA fingerprint spectrum consists of 61 molecular markers which are distributed in 5 chromosomes, are positioned at exons and are developed by SNP function loss type mutation, wherein the 61 molecular markers cause early termination or termination gain of codons; a primer pair combination corresponding to the 61 molecular markers is composed of primers as shown in SEQ ID NO. 1 to 122. The invention further provides application, and the application is to identify peony varieties' Hua powder new makeup 'and' Henyan '; identifying true and false hybrids of two-by-two filial generations of 'Paeonia ostii', 'Hua flour Xinmakeup' and 'Henyan'; and peony germplasm resource genetic diversity and peony variety resource evolutionary tree analysis. The primer pair combination of the SNP molecular marker combination is used for genetic diversity analysis, identification and germplasm resource utilization; and identifying true and false hybrids of filial generations of the peony male and female parents with different genotypes on a plurality of SNP sites.

The application provides a method for breeding a pure white color Wuzhishan pig. The method adopts a strategy of phenotype breeding as the main part and molecular marker monitoring as the auxiliary part, simultaneously uses KIT gene detection to confirm the genotype background of each generation, uses SSR genetic diversity monitoring to guide scientific mating and external blood introduction, avoids inbreeding recession, and through breeding, a new strain of Wuzhishan pig with pure white color, stable heredity and rich blood is obtained.

This invention discloses a primer set for SSR molecular markers in *Pterocarya stenoptera* and its applications. By analyzing *Pterocarya stenoptera* germplasm samples from different regions, this invention obtained SSR molecular markers that can be used for the identification of the germplasm source (property) of *Pterocarya stenoptera*. Based on this, this invention also provides a primer set for SSR molecular markers in *Pterocarya stenoptera*. This primer set can not only be used for the identification of the propagation of *Pterocarya stenoptera*, but also for the analysis of genetic diversity and the construction of genetic linkage maps, exhibiting advantages such as high polymorphism, good repeatability, marker stability, and clear and easily interpretable banding patterns. Furthermore, this invention also provides a detection kit for SSR molecular markers in *Pterocarya stenoptera*, which can be used for the identification of the propagation of *Pterocarya stenoptera*, the construction of DNA fingerprint maps, and the evaluation of genetic diversity and phylogenetic studies, thus contributing to the molecular-assisted breeding of *Pterocarya stenoptera*.