237 results about "Genetic diversity" patented technology

The invention discloses a sugarcane germplasm resource evaluation and breeding method for smart agriculture. The sugarcane germplasm resource evaluation and breeding method comprises the following steps: step 1, collecting materials with wide genetic diversity; establishing an intelligent incubator and a greenhouse, and monitoring environmental parameters by using an Internet of Things sensor; 2, performing data acquisition on the related germplasm resources by using an unmanned aerial vehicle, a robot and a near infrared spectrum technology, and constructing a phenotype database for data management; meanwhile, genotype identification is carried out by combining molecular marker-assisted selection and genome selection technologies, molecular markers of key genes are mined, and the genetic value of related germplasm is evaluated; step 3, constructing a hybrid combination prediction model by using an AI algorithm, optimizing parent matching of the related germplasm and the cultivated sugarcane, monitoring and collecting the filial generation at the same time, and analyzing and screening in combination with smart agriculture; 4, demonstration planting is conducted on the bred excellent sugarcane strain, and application of the excellent strain is improved through government-enterprise-scientific research institution collaborative popularization and in combination with farmer technical training.

The invention discloses a cowpea 5K SNP (Single Nucleotide Polymorphism) liquid chip and application thereof in variety identification, and belongs to the technical field of biology. The liquid chip provided by the invention comprises a set of nucleotide probes for detecting 5847 SNP sites of cowpea, and can be widely applied to different scenes of germplasm resource identification, genetic diversity evaluation, variety identification, genetic relationship identification, purification and rejuvenation of local varieties and the like of different cowpea varieties; an effective scientific basis is provided for cowpea variety identification, the variety identification and evaluation efficiency is improved, and the method has a remarkable application value.

The invention discloses a tea tree liquid phase chip and application thereof, and relates to the technical field of molecular detection. The invention discloses a tea tree liquid phase chip and application thereof, according to site screening requirements and probe design principles, the tea tree liquid phase chip comprises 5781 SNP sites, and tea tree resource genetic typing can be realized based on a target interval genome sequence liquid phase capture accurate positioning sequencing typing technology. The tea tree liquid chip can realize low-cost genetic typing, is mainly specific to tea trees, can realize variety identification and genetic relationship analysis of the tea trees, scientifically guides hybridization improvement work of the tea trees and assists protection and development of germplasm resources of the tea trees, and has relatively high application values in multiple fields of tea tree breeding. The tea tree liquid phase chip can be used for tea tree genetic diversity evaluation, germplasm resource and genetic relationship identification, genetic map construction and gene localization, whole genome association analysis and tea tree molecular marker assisted breeding.

The invention provides an SNP (Single Nucleotide Polymorphism) site combination for genetic typing of micropterus salmoides and application of the SNP site combination. The SNP locus combination comprises 50000 SNP loci, and the positions and base information of the 50000 SNP loci on chromosomes are as shown in a table 3. By applying the micropterus salmoides whole genome liquid chip of the SNP site combination for genotyping of micropterus salmoides, provided by the invention, the genotype of a sample to be detected can be rapidly and accurately detected, and the average detection rate of the SNP sites and the average consistency rate of the genotype of the sample are as high as 99.95%; the micropterus salmoides whole genome liquid phase chip has the characteristics of high capture specificity, low cost, flexible sites and the like, is suitable for character breeding and improvement, gene mining, genetic relationship identification, genetic diversity analysis and the like of micropterus salmoides, and can effectively improve the breeding efficiency and accuracy of improved varieties of micropterus salmoides; and a new variety of largemouth micropterus salmoides which is more suitable for market requirements and is more competitive can be cultivated.

The invention relates to a gene related to the body color character of paramisgurnus dabryanus, a primer group of an SNP marker and application of the primer group, and belongs to the technical field of molecular markers, and the nucleotide sequence of the gene is as shown in SEQ ID NO.1. The invention also provides a primer pair for detecting the body color related SNP marker herc2-105, and the nucleotide sequence of the primer pair is as shown in SEQ ID NO: 2-3; the SNP marker herc2-105 is located at the 105bp position of the nucleotide sequence of the gene, and the polymorphic form of the SNP marker herc2-105 is A / T. Wherein the AA genotype individual has a golden red body color, and the AT and TT genotype individuals have a wild body color. The molecular marker provided by the invention can be used for body color character breeding of the golden red paramisgurnus dabryanus, the genetic diversity of population offspring is effectively increased, and the breeding process of new body color variety breeding of the red paramisgurnus dabryanus is remarkably promoted.

The invention discloses a pig breeding screening method and system based on a body type database, and particularly relates to the technical field of pig breeding screening. The system comprises a multi-modal sign data acquisition and integration module for collecting and standardizing heterogeneous data sources such as phenotypic signs, genomics and environmental parameters of breeding pigs in real time, and a multi-dimensional biological characteristic modeling and analysis module for analyzing a nonlinear action mechanism among genetic markers, phenotypic characteristics and environmental factors, the genetic potential intelligent judgment module is used for quantitatively evaluating spatial expression potential and intergenerational transmission probability of genetic advantages of the breeding pigs through a deep learning algorithm; and the intelligent screening and dynamic monitoring module is used for establishing a multi-dimensional threshold dynamic regulation and control mechanism to realize accurate elimination decision and continuous optimization of a germplasm resource library. According to the method, the screening accuracy is remarkably improved by designing multi-source data fusion and online optimization capability, meanwhile, mistaken elimination is reduced through a redundant superior species temporary storage management module, and collaborative improvement of population genetic diversity and environmental adaptability is ensured.

The invention belongs to the technical field of molecular biology and plant molecular breeding, and particularly relates to a mustard core SNP molecular marker set and a screening method and application thereof. The invention provides a core SNP marker set (33) which is subjected to whole genome re-sequencing screening and experimental verification and is suitable for mustard germplasm resource identification and genetic analysis for the first time. The marker set is high in polymorphism and good in stability, covers the whole genome and can effectively distinguish different leaf mustard germplasms. By utilizing the core marker set and the matched KASP primer, the genetic typing of the leaf mustard germplasm resources can be quickly and accurately performed with high throughput, and the defects that the traditional morphological identification is time-consuming, labor-consuming and poor in accuracy are overcome. The marker set can be used for analyzing the genetic diversity, the population structure and the phylogenetic relationship of the leaf mustard germplasm resources, and a molecular basis is provided for collection, preservation and evaluation of the germplasm resources and breeding parent matching.

Provided are a Medicago sativa SNP molecular marker combination and a use thereof. The SNP molecular marker combination consists of 61351 SNP molecular markers. The physical positions of the 61351 SNP molecular markers are determined by sequence alignment on the basis of the Medicago sativa reference genome ZM-4 alfalfa genome. The SNP molecular markers have good site specificity, strong versatility, and high polymorphism and can effectively discriminate Medicago sativa germplasms from different sources. A Medicago sativa genome-wide liquid-phase gene chip is prepared from the SNP molecular markers, and can be used for genetic diversity assessment of Medicago sativa germplasm resources, genetic map construction and functional gene mapping, genome-wide association study, etc., providing an important tool and technical support for Medicago sativa molecular design breeding and variety authenticity identification, and achieving important significance for high-quality development of the alfalfa seed industry in China.

The invention belongs to the technical field of molecular biology, and particularly discloses a cotton 10K functional site breeding chip and application thereof. The breeding chip is named as' Cotton Core No.1 ', and comprises a 10K functional site targeting capture probe group designed based on a reference genome of disease-resistant, high-quality and high-yield modern upland cotton No.8, the probe group comprises 11159 SNP (Single Nucleotide Polymorphism) loci, 3981 of the SNP loci are functional loci associated with 13 important agronomic characters, and 7178 of the SNP loci are background loci reflecting genetic diversity; the SNP site information is shown in the specification table 1. The invention discloses a cotton 10K functional site breeding chip and application thereof, the breeding chip contains functional sites with more index function genetic variation, has higher detection accuracy among different cotton varieties, is applied to cotton molecular marker-assisted selection breeding, whole genome selection breeding and whole genome correlation analysis, and has higher detection accuracy. The breeding efficiency can be obviously improved, and the practical application is wider.

The invention relates to the technical field of species distribution analysis, and discloses a dove tree population natural update limitation-based species distribution analysis system, which comprises a habitat factor acquisition module, an update limitation evaluation module, a distribution prediction module, a spatial constraint correction module and an adaptability atlas optimization module. The habitat factor acquisition module acquires data in real time and constructs a multi-layer environment factor topology; the update limit evaluation module evaluates update limit in combination with genetic diversity and seed diffusion constraint; the distribution prediction module generates a distribution strategy through spatial modeling and ecological niche optimization; the spatial constraint correction module corrects a diffusion range and genetic parameters based on terrain and climate factors; and the adaptability graph optimization module feeds back an analysis result to carry out closed-loop optimization on the strategy. The system is provided with a multi-stage obstacle avoidance mechanism and a redundant link, so that the habitat change response capability is improved. According to the method, accurate analysis and dynamic optimization of davidia involucrata distribution are realized, and technical support is provided for species protection.

The invention belongs to the technical field of whole genome gene chips, and particularly relates to a cattle whole genome liquid phase chip and application thereof. Based on an SNP molecular marker combination (position information is shown in a specification table 1) provided by the invention, cattle genotyping can be realized through a targeted capture sequencing technology, and cattle genotypes can be detected in a short time and at high throughput, so that cattle breeding work is promoted. Furthermore, based on the cattle SNP molecular marker combination provided by the invention, a probe is designed, and a whole genome breeding chip is constructed, so that large-scale typing site detection rate of cattle is high, stability is good, and platform wide adaptability is achieved. The method can be applied to germplasm resource identification and mining, genetic diversity analysis, population structure analysis, genetic relationship identification and whole genome selective breeding of cattle varieties, especially Anhui province local cattle varieties, and has important significance in improving the cattle core provenance autonomous breeding efficiency.

The invention discloses a compression and decompression method based on generic genome expression, and relates to the technical field of compression and decompression of DNA next-generation sequencing data, in particular to the compression and decompression method based on generic genome expression. The method aims at solving the problems that in the prior art, the capacity of processing population genetic diversity is insufficient, original sequencing quality information cannot be effectively restored during decompression, and memory occupation is too high during large-scale data processing. Obtaining a to-be-compressed sequencing sequence data file, a reference genome sequence and a thousand-person genome variation sample; obtaining a haplotype list, a variation list and a haplotype offset list corresponding to each window block; storing the window number, the haplotype number, the haplotype offset, the head and tail unmatched sequences, the current sequence name and the quality score character string into a single compression block; carrying out binding storage; completing the compression processing of the mass fraction; and obtaining each to-be-compressed sequencing sequence based on the result of the compressed part.

The invention discloses a sesbania SSR primer combination developed based on a shallow genome sequence and application thereof, and belongs to the technical field of molecular markers. The invention develops an SSR primer combination based on a shallow genome sequence of sesbania, and the SSR primer combination comprises 23 pairs of SSR primer pairs. The SSR primer pair developed by the invention has the advantages of high polymorphism, rich polymorphic information content, stable amplification result, high resolution, accuracy and the like, can quickly and accurately judge the source and variety of sesbania, is beneficial to the research of germplasm resource identification, germplasm resource genetic diversity analysis, genetic structure analysis and the like of sesbania, and has wide application prospects. The method has important significance in promoting conservation and utilization of sesbania germplasm resources and promoting industrial development.

The invention relates to the technical field of molecular genetic breeding, and particularly discloses an eggplant core SNP molecular marker set and application, and the molecular marker set comprises 23 SNP markers; screening representative eggplant germplasm for re-sequencing; the method comprises the following steps: by taking an eggplant HQ-1315V1.0 genome as a reference genome, carrying out comparison to obtain 4869502 SNP (Single Nucleotide Polymorphism) markers; the method comprises the following steps: screening to obtain a first SNP molecular marker set based on a material discrimination degree, a distribution uniformity degree on a genome, specificity intensity, a heterozygosity degree, a gene diversity index, a polymorphic information amount PIC and a minimum allele frequency MAF principle of SNP markers; 96 SNP molecular markers are randomly selected, and genetic typing verification is carried out on the SNP molecular markers by using eggplant resource materials to obtain 32 candidate SNP molecular markers; and further performing genetic diversity evaluation, clustering analysis and population structure analysis on the 32 candidate SNP molecular markers, and screening to obtain 23 core SNP molecular marker sets. The method has the characteristics that germplasm resources can be effectively distinguished, and variety identification and authenticity detection are convenient.

The invention belongs to the technical field of molecular biology, bioinformatics, sorghum genetics and whole-genome gene chips, and particularly relates to a sorghum whole-genome SNP molecular marker combination developed based on a targeted sequencing technology, a 10K liquid chip and application of the sorghum whole-genome SNP molecular marker combination and the 10K liquid chip. The SNP molecular marker combination comprises 10,000 SNP loci, and the specific information of the 10,000 SNP loci is as shown in a table 1; the physical positions of the 10000 SNP loci are determined by performing sequence alignment based on a reference genome of the wine red tassel sorghum variety HYZ-T2T with the version number of v1.0. A liquid chip developed based on the SNP molecular marker combination provided by the invention can be effectively applied to genetic diversity analysis, whole genome association analysis, genetic typing, molecular marker-assisted selective breeding, whole genome selective breeding and other applications of sorghum germplasm resources.

The invention belongs to the field of microbial breeding and fermentation engineering, and particularly relates to a saccharomyces cerevisiae mutagenesis strain with high yield of S-adenosyl-L-methionine as well as a mutagenesis method and application of the saccharomyces cerevisiae mutagenesis strain. Saccharomyces cerevisiae is induced to generate genetic diversity through multiple rounds of ultraviolet mutagenesis, and high-throughput screening is performed in combination with strain color phenotypic difference; and the mutant strain HY2402-Z18 with high yield of S-adenosine-L-methionine and genetic stability is obtained. The SAM yield of the mutagenic strain is increased by 40.1% compared with that of an original strain, the SAM yield reaches 13.44 g / L when the mutagenic strain is fermented for 60 h in a 5 L fermentation tank system, the unit yield reaches 0.156 g / L / OD, and the methionine conversion rate is 75%. After thalli are collected through centrifugation of fermentation liquor, an S-adenosine-L-methionine product with the purity of 98%-100% can be obtained through purification of ion exchange resin, no by-products are accumulated, the yield, the substrate conversion rate and the production efficiency of the S-adenosine-L-methionine can be remarkably improved when the mutagenesis strain is used for fermentation production, and the production cost is reduced. The production cost is effectively reduced, the product purity and quality are improved, and the method is suitable for industrial large-scale production of SAM.

This invention relates to the fields of genomics, molecular biology, bioinformatics, and genome-wide selection breeding, specifically a 20k liquid-phase breeding chip for the whole genome of *S. esculenta* and its applications. The liquid-phase chip contains background SNPs and functional SNPs located on the *S. esculenta* reference genome; wherein the background SNPs are uniformly distributed within the genome; and the functional SNPs are associated with important economic traits of *S. esculenta*; these important economic traits include one or more of the following: saponin content, polysaccharide content, and heat tolerance. The chip can be applied to the assessment of genetic diversity in *S. esculenta*, identification of germplasm resources and phylogenetic relationships, genome-wide association analysis of important economic traits, and genome-wide selection breeding. This chip has advantages such as high throughput, high region coverage, high locus detection rate, and high flexibility, providing powerful tool support for molecular breeding of *S. esculenta*.

The invention discloses a corn genome breeding 45K chip and application thereof, the chip covers 44, 637 SNP loci and 27 InDel loci, and the SNP loci are uniformly distributed on 10 chromosomes and part of scaffold of a corn genome and have rich polymorphism. Compared with an existing corn chip, the SNP marker of the 45K chip is derived from re-sequencing results of 507 corn varieties, has better variety representativeness, and contains 925 key functional gene loci for close planting and high yield and 96 corn variety authenticity identification loci; the method can be more effectively used for functional gene identification, genetic diversity analysis, heterosis group division, whole genome selection analysis and variety authenticity identification.

The invention provides a tilia amurensis primer combination, a DNA fingerprint spectrum, a variety identification method and application thereof, and relates to the field of molecular biology and plant genetics, the primer combination is a primer combination of an SSR molecular marker, and comprises a primer C110, a primer C840, a primer D150 and a primer TC5, and the primer sequences are shown as SEQ ID NO.1 to SEQ ID NO.8. The tilia amurensis primer combination is used for genetic diversity analysis of tilia amurensis, has the characteristic of high polymorphism, and can be used for identifying the variety of tilia amurensis. 70 polymorphic sites can be detected, different tilia amurensis individuals can be distinguished, the tilia amurensis DNA fingerprint spectrum can be constructed only through four pairs of primers, and the method is suitable for tilia amurensis germplasm resource protection, endangered population monitoring and molecular breeding.

The invention discloses an efficient extraction method of blue fox sperm DNA, and belongs to the technical field of molecular biology. Aiming at the structural characteristics that chromatin of the blue fox sperms is highly condensed, histone is replaced by protamine, and a stable nucleoprotein complex is formed through an intermolecular disulfide bond, the invention provides an efficient DNA extraction method suitable for the blue fox sperms. According to the method, based on the synergistic effect of three chemical reagents, namely SDS, PK and DTT, full lysis of the sperm cells of the blue foxes is achieved, and then efficient enrichment of sperm DNA of the blue foxes can be achieved by combining a conventional DNA extraction method. According to the efficient extraction method of the blue fox sperm DNA provided by the invention, the yield and integrity of the blue fox sperm DNA are remarkably improved, and the limitation that sperm chromatin is difficult to effectively lyse by a conventional method is overcome; the method provides reliable technical support for research on genetic diversity evaluation, population management, molecular breeding and the like of the blue foxes, and has the potential of popularization and application in other high-condensation sperm species.

The invention relates to a biodiversity charting method and device, electronic equipment and a storage medium, and the method comprises the steps: obtaining remote sensing observation data, foundation observation data and species information data, constructing an ecological data cube, extracting statistical features, frequency features and phenological features according to the ecological data cube, and calculating the statistical features, the frequency features and the phenological features according to the statistical features, the frequency features and the phenological features; the method comprises the following steps: constructing a species diversity space map, a phylogenetic diversity map and a global genetic diversity map, fusing the maps to obtain a fusion result, identifying a high-diversity region and a low-protection region according to the fusion result, and evaluating the high-diversity region and the low-protection region to obtain an evaluation result. Therefore, the problems that related technologies depend on the number of samples and data precision, phylogenetic and genetic layer patterns are difficult to reveal, and global scale decision and management requirements are difficult to meet are solved, multi-source satellite remote sensing and foundation data can be fused, and the comprehensiveness and scientificity of diversity evaluation are improved.

The invention belongs to the technical field of biology, and particularly relates to a sophora tonkinensis SSR molecular marker primer group, a kit as well as application and a method of the sophora tonkinensis SSR molecular marker primer group. The sophora tonkinensis SSR molecular marker primer group comprises 15 pairs of primers, the nucleotide sequences of the 15 pairs of primers are sequentially shown as SEQ ID NO.1-SEQ ID NO.30, and the upstream primer in each primer pair is marked with a fluorophore. The sophora tonkinensis SSR molecular marker primer provided by the invention is high in polymorphism, and can effectively reveal the genetic diversity of sophora tonkinensis; a fingerprint database of sophora tonkinensis germplasm is also constructed, and the variety of sophora tonkinensis to be detected can be identified through comparison; the number of the SSR molecular markers of the sophora tonkinensis is increased, a tool is provided for genetic diversity research and germplasm resource analysis of the sophora tonkinensis, and a theoretical basis is provided for protection and utilization of sophora tonkinensis resources.

The present invention discloses a SNP molecular marker associated with the testicle size trait of Hu sheep, its screening method, and application, belonging to the fields of genetic breeding and molecular biology. The marker is located in the intron region of the LRRIQ1 gene on chromosome 3 of Hu sheep and is significantly associated with the testicle size trait of Hu sheep. Through a scientific and rigorous screening method, the accuracy and reliability of the SNP molecular marker are ensured; the marker can quickly and accurately screen out Hu sheep individuals associated with the testicle size trait, improve breeding efficiency and accuracy, accelerate the breeding process, reduce breeding costs, and enhance economic benefits; it helps to protect genetic diversity and improve the accuracy of semen quality evaluation, providing a powerful tool for Hu sheep breeding work and important basic data for studying the genetic mechanism of testicular development and function; it can also be used as an auxiliary diagnostic tool to assist in identifying potential genetic defects or disease risks, promoting the progress and development of breeding technology.

The invention relates to the technical field of plant molecular markers, in particular to a millet whole genome 65K liquid chip and application thereof.The millet whole genome 65K liquid chip is obtained through the following steps that re-sequencing data of a millet material is obtained, and then the re-sequencing data is subjected to quality control, mapping with a reference genome and identification and screening of variation sites; according to the indexes of the variation sites, combining the positions of the sites on the chromosomes, analyzing upstream and downstream sequences of the variation sites, and selecting the variation sites which can be used for chip development; the obtained variation site and 50bp sequences before and after the variation site are used, and an Infinium chip manufacturing technology is used for manufacturing the millet whole genome 65K liquid phase chip; based on high-depth whole genome re-sequencing data of large-scale millet germplasm materials, the millet whole genome 65K liquid chip is successfully developed, and can be widely applied to the fields of millet genotype detection, variety identification, genetic positioning, genetic diversity analysis, whole genome correlation analysis, whole genome selective breeding and the like. And important technical support is provided for molecular breeding development of millet.

The invention discloses a blocky coral breeding and transplanting combined device and method, and relates to the technical field of artificial coral reefs, and the blocky coral breeding and transplanting combined device comprises a base support with a through hole and a bearing surface; the fixing piece is provided with a plurality of mounting holes and connecting holes; the block-shaped coral is provided with a round hole; the connecting piece is provided with a protruding part, and the protruding part penetrates through the round hole to fix or press the block-shaped coral on the base support and extends to the connecting hole to fix the base support on one face or two faces of the fixing piece. Modular design is adopted, all parts can be flexibly combined so as to improve the operation efficiency, and large-scale carrying, transportation and production are facilitated. The operation efficiency of submarine divers can be effectively improved, and meanwhile the survival rate of coral transplantation and transportation in different places is increased. In addition, the problem that blocky coral varieties cannot be transplanted through a traditional transplanting method is solved, and the genetic diversity of coral repair is greatly improved.

The invention relates to the technical field of gene detection, and particularly discloses an SNP (Single Nucleotide Polymorphism) marker combination, application of the SNP marker combination in genetic relationship identification and / or genetic diversity analysis of monkey flock, and a primer sequence. The SNP marker combination comprises 196 SNP molecular markers, site information of the 196 SNP molecular markers is shown in a table 1, and primer sequences of the 196 SNP molecular markers are shown in a table 4. The SNP marker combination provided by the invention has the characteristics of stability, economy and high efficiency on SNP site detection of experimental monkeys, and can be used for genetic relationship identification and / or genetic diversity analysis of monkey flock.

The present invention discloses a set of SSR molecular markers for identifying wild chrysanthemum and Shennong fragrant chrysanthemum germplasm resources, consisting of 21 pairs of SSRs numbered SSR01-SSR21. The primer sequences for amplifying these 21 pairs of molecular markers are shown in SEQ ID NO: 1-42, and the present invention belongs to the field of molecular biology technology. Using these 21 pairs of SSR molecular markers, the collected wild chrysanthemum and Shennong fragrant chrysanthemum can be identified and the germplasm genetic diversity analyzed. The present invention uses SSR markers for the first time to jointly detect the genetic diversity of wild chrysanthemum and Shennong fragrant chrysanthemum germplasm. This set of SSR molecular markers has good polymorphism and a high level of genetic diversity. It can be used in the fields of kinship analysis, resource genetic evaluation, identification of interspecific hybrid offspring, positioning of trait genes, and molecular marker-assisted breeding of wild chrysanthemum and Shennong fragrant chrysanthemum.

The invention discloses an SNP (Single Nucleotide Polymorphism) marker related to a golden red paramisgurnus dabryanus body color character and application thereof, and relates to the technical field of molecular markers. The SNP marker is located at the 1354bp position of an rp2 gene of a No.7 chromosome of paramisgurnus dabryanus, the polymorphic form of the SNP marker is A / G, an individual of an AA genotype has a golden red body color, and individuals of AG and GG genotypes have a wild body color. The invention further provides a primer pair and a kit for detecting the SNP marker and application of the primer pair and the kit in detecting the body color character of the golden red paramisgurnus dabryanus and breeding. The nucleotide sequence of the primer pair is shown as SEQ ID NO: 2-SEQ ID NO: 3. The molecular marker provided by the invention can be used for detecting the body color of the paramisgurnus dabryanus and breeding the body color character of the golden red paramisgurnus dabryanus, so that the genetic diversity of population offspring is effectively increased, and the breeding process of breeding of a new variety of the body color of the red paramisgurnus dabryanus is remarkably promoted.

The present application relates to the technical field of molecular marker development and molecular detection, discloses development of dwarf tomato InDel molecular marker primer, provides a group of dwarf tomato InDel molecular markers, the dwarf tomato InDel molecular marker includes 79 InDel markers shown in the following table, and the dwarf tomato InDel molecular marker is determined with reference to genome SL5.0.Using the dwarf tomato InDel molecular marker can provide technical support for carrying out target DNA sample rapid molecular detection in dwarf tomato;Since the set of markers is distributed on multiple chromosomes of the dwarf tomato genome, can be selected and used according to actual needs, the genomic DNA of the candidate material can be detected at any stage, has the advantages of flexible use, simple operation, high detection efficiency, less limiting factors, stable and accurate detection results, provides a simple, flexible and efficient way for genetic linkage map construction, genetic diversity analysis, resource identification, hybrid / purity identification and the like in the dwarf tomato.

The invention relates to the technical field of molecular marker identification, and discloses a set of InDel molecular markers for identifying a wide-peel citrus variety and application of the InDel molecular markers, the InDel molecular markers comprise 42 InDel sites, each site is in two states, and each marker represents a chromosome site; 42 pairs of InDel molecular marker primers are correspondingly arranged on the 42 InDel sites, and the nucleotide sequences of the primers are shown as SEQ ID NO. 1 to SEQ ID NO. 84 in a sequence table. The 42 citrus InDel loci provided by the invention are stable in typing, have space-time crossing performance and can be used for constructing a citrus InDel fingerprint database. The method is beneficial to citrus variety identification, classification, germplasm resource protection and genetic diversity research.