8 results about "Pacific oyster" patented technology

The invention relates to the field of biological product preparation, in particular to a preparation method of high-activity oyster-derived small molecule peptide. The method comprises the following steps: selecting fresh meat of pacific oysters or ostrea rivularis as a raw material, degreasing, adding water, crushing, filtering, sequentially carrying out compound protease and neutral protease hydrolysis, implanting a sensor and a probe in hydrolysis, monitoring in real time, and dynamically adjusting reaction conditions; adding a targeting microcapsule wrapping an ascorbic acid glutathione composite protective agent, and releasing the protective agent when a target peptide signal is detected; separating and concentrating through a gradient membrane, and purifying the target peptide by using a molecular imprinting chromatographic column; carrying out alkaline hydrolysis on enzymatic hydrolysis residues to prepare chitosan for cyclic utilization, carrying out enzymatic hydrolysis on residual residues to generate collagen peptide, treating and recycling wastewater, and recovering protease. According to the method, the technical effects that the high-activity and high-purity target peptide is prepared, the resource utilization rate is increased, the production cost is reduced, the target peptide has good oxidation resistance and biological activity, the intestinal enzyme degradation rate is reduced after glycosylation modification, and the blood half-life period is prolonged are achieved.

The present application relates to the field of biological medicine, and discloses a method for repairing female reproductive tract based on marine active substances, comprising the following steps: S1, providing a composition containing marine active substances, wherein the marine active substances comprise sea cucumber saponins, oyster polypeptides and brown alga polysaccharides; S2, formulating the composition into a topical gel preparation; S3, applying the topical gel preparation to the female reproductive tract through vaginal or intrauterine administration to perform endometrial regeneration, scar reduction or inflammation microenvironment improvement, wherein the sea cucumber saponins are derived from Holothuria scabra; the oyster polypeptides are derived from Crassostrea gigas; and the brown alga polysaccharides are derived from Sargassum thunbergii and are sulfated polysaccharides. By combining sea cucumber saponins, oyster polypeptides and brown alga polysaccharides in a specific ratio (0.5-1.2%, 1-2.5%, 2-4%), the repairing effect of the female reproductive tract is significantly improved, which is superior to single-component gel or existing hyaluronic acid gel.

This invention discloses a method for accurately assessing the germplasm purity of Fujian oysters, comprising the following steps: Step 1, acquiring high-coverage whole-genome resequencing data of the oyster individual to be tested; Step 2, comparing the resequencing data with the Fujian oyster reference genome and identifying the nucleotide base types of the individual to be tested; Step 3, assigning a score to each specific single nucleotide polymorphism site and accumulating the scores of all sites to obtain a total score; Step 4, calculating the Fujian oyster germplasm purity value of the individual to be tested based on the total score. This invention utilizes high-coverage genome resequencing to screen for single nucleotide polymorphism sites throughout the Fujian oyster genome and compares them with the genome sequence of Pacific oyster to identify highly reliable Fujian oyster genome-specific nucleotide bases, constructing a purebred Fujian oyster genome sequence site set, and achieving efficient and accurate quantitative assessment of Fujian oyster germplasm purity by calculating the percentage of purebred sites in the Fujian oyster genome.

This invention discloses a method for identifying the parentage of tetraploid Pacific oysters based on SNP loci, belonging to the field of molecular-assisted breeding technology for tetraploid Pacific oysters. The method includes the following steps: (1) sequencing the adductor muscle of the tetraploid Pacific oyster to be tested; (2) comparing the genotype of the designated SNP locus in the tetraploid Pacific oyster with the reference genome; (3) calculating the IBS and LOD of all tested parents and offspring based on the SNP typing results; and (4) combining the IBS and LOD to identify the parentage of the tested parents and offspring. The advantages of this invention are: it discloses for the first time 1000 SNP loci for identifying the parentage of tetraploid Pacific oysters, providing data support for the identification of parentage in tetraploid Pacific oysters and laying the foundation for the development of liquid-phase chips for identifying the parentage of tetraploid Pacific oysters, showing good application prospects.

The invention discloses a recombinant crassostrea gigas complement C3 cleavage fragment rCgC3dg as well as a preparation method and application thereof. The amino acid sequence of the rCgC3dg is shown as SEQ ID NO. 1; the preparation method comprises the following steps: carrying out PCR (Polymerase Chain Reaction) amplification on a Crassostrea gigas CgC3dg coding region fragment by using primers P1 and P2, wherein the DNA (Deoxyribose Nucleic Acid) sequence of the primer P1 is as shown in SEQ ID NO. 2, and the DNA sequence of the primer P2 is as shown in SEQ ID NO. 3; respectively carrying out BamHI and Xhol I enzyme digestion on a PCR amplification product and a pET-30a carrier, and then connecting and transforming through T4 ligase to construct a recombinant; transferring the recombinant into an escherichia coli Transsetta (DE3) expression strain, culturing the expression strain, adding IPTG (isopropyl-beta-d-thiogalactoside) to carry out induced expression on the recombinant protein, and then purifying and renaturating to obtain the recombinant rCgC3dg protein. The recombinant crassostrea gigas complement C3 split fragment rCgC3dg and rCgGSDME-N can be used for preparing a medicine for resisting vibrio splendidus and escherichia coli after being mixed.

The application discloses a breeding method of a hybrid triploid oyster new strain which is fast-growing and high-resistant. The hybrid triploid oyster new strain is obtained through the following technical links: breeding of a high-resistant and fast-growing diploid strain, establishment of a high-resistant and fast-growing tetraploid induction group strain, breeding of a high-resistant and fast-growing tetraploid new strain, breeding of a high-resistant and fast-growing hybrid diploid new strain, batch production of a high-resistant and fast-growing hybrid triploid new strain, and the like, combined use of population selection breeding, family selection breeding, stress breeding, hybrid breeding and polyploid breeding technologies. The hybrid triploid oyster new strain is fast-growing, high-resistant, adaptive to the habitat of North China sea area, poor in fertility, good in quality and high in fullness degree throughout the year, and can effectively solve the problems of high mortality, slow growth, high breeding risk and low benefit of Pacific oysters in North China sea area, and has remarkable popularization significance and value.

This invention discloses a recombinant serum amyloid A (r) protein from the Pacific oyster. Cg SAA), preparation method and application, r Cg The SAA amino acid sequence is shown in SEQ ID NO. 1; the preparation method is to use primers P1 and P2 to... Cg The SAA coding region fragment was amplified by PCR, and the DNA sequences of primers P1 and P2 are shown in SEQ ID NO. 2 and SEQ ID NO. 3, respectively. The PCR amplification product was then mixed with the pET-30a vector via... Bam HI and Xhol After digestion with enzyme I, ligation was performed using T4 ligase, followed by transformation to construct recombinants; the recombinants were then transformed into *E. coli*. Transetta In the (DE3) expression strain, the expression strain was then cultured and IPTG was added to induce expression, followed by purification and refolding to obtain r. Cg SAA protein. The prepared r Cg SAA inhibits the growth of Vibrio splenti and Vibrio anguillarum under non-acidic conditions, and can also kill Vibrio splenti. It can be used to prepare drugs against Vibrio splenti and Vibrio anguillarum, and can be applied directly, effectively and safely to aquaculture.

The invention discloses pacific oyster galactose / rhamnose binding lectin CGL-1 recombinant protein as well as a preparation method and application thereof, and belongs to the technical field of biological medicines. In a diabetic rat model, the CGL-1 recombinant protein can significantly promote the healing of MRSA infectious wounds, and the healing rate reaches 92.44%-94.51%. Histological analysis shows that inflammatory cell infiltration of a wound surface of a CGL-1 treatment group is reduced, collagen deposition is increased, neovascularization is good, and a good healing effect is shown. In addition, the bacterial quantity of a wound surface of a CGL-1 treatment group is obviously lower than that of a model group, and the bacterial quantity is respectively reduced by 25.3%-88.55% and 68.45%-95.83% on the fifth day and the thirteenth day. The results show that the CGL-1 lectin protein has the potential of serving as a novel non-antibiotic therapeutic agent, can be used for treating diabetes infected wounds, and provides a new thought for application of marine biological resources in the field of wound healing.