The invention relates to an induction method for a 'Haida 2#' new variety tetraploid of crassostrea gigas. The induction method comprises the steps of selecting a 'Haida 2#' new variety of crassostreagigas as parent oysters, carrying out manual maturity-promoting culture until the parent oysters are mature, selecting female and male individuals with good gonad development as parent oysters, dissecting for fetching ova, filtering, putting the ova into filtered seawater for maturation, enabling the maturated ova to be fertilized with sperms in the filtered seawater, processing fertilized ova byvirtue of cytochalasin B so as to inhibit the discharging of first polar bodies, after the processing, collecting the fertilized ova, transferring the fertilized ova into an ethanol solution for soaking, finally transferring the fertilized ova into a culture container, and carrying out incubation and larva culture. According to the induction method, the discharging of the first polar bodies of the fertilized ova of the ''Haida 2#'' new variety of a diploid is inhibited by virtue of the cytochalasin B, and the living 'Haida 2#' new variety tetraploids of crassostrea gigas are directly induced,so that the survival rate is greatly increased, and a seedling culturing foundation is provided for the amplified breeding of the 'Haida 2#' new variety tetraploid of crassostrea gigas.