3 results about "Pacific oyster" patented technology

This invention discloses a method for accurately assessing the germplasm purity of Fujian oysters, comprising the following steps: Step 1, acquiring high-coverage whole-genome resequencing data of the oyster individual to be tested; Step 2, comparing the resequencing data with the Fujian oyster reference genome and identifying the nucleotide base types of the individual to be tested; Step 3, assigning a score to each specific single nucleotide polymorphism site and accumulating the scores of all sites to obtain a total score; Step 4, calculating the Fujian oyster germplasm purity value of the individual to be tested based on the total score. This invention utilizes high-coverage genome resequencing to screen for single nucleotide polymorphism sites throughout the Fujian oyster genome and compares them with the genome sequence of Pacific oyster to identify highly reliable Fujian oyster genome-specific nucleotide bases, constructing a purebred Fujian oyster genome sequence site set, and achieving efficient and accurate quantitative assessment of Fujian oyster germplasm purity by calculating the percentage of purebred sites in the Fujian oyster genome.

The application discloses a breeding method of a hybrid triploid oyster new strain which is fast-growing and high-resistant. The hybrid triploid oyster new strain is obtained through the following technical links: breeding of a high-resistant and fast-growing diploid strain, establishment of a high-resistant and fast-growing tetraploid induction group strain, breeding of a high-resistant and fast-growing tetraploid new strain, breeding of a high-resistant and fast-growing hybrid diploid new strain, batch production of a high-resistant and fast-growing hybrid triploid new strain, and the like, combined use of population selection breeding, family selection breeding, stress breeding, hybrid breeding and polyploid breeding technologies. The hybrid triploid oyster new strain is fast-growing, high-resistant, adaptive to the habitat of North China sea area, poor in fertility, good in quality and high in fullness degree throughout the year, and can effectively solve the problems of high mortality, slow growth, high breeding risk and low benefit of Pacific oysters in North China sea area, and has remarkable popularization significance and value.

This invention discloses a recombinant serum amyloid A (r) protein from the Pacific oyster. Cg SAA), preparation method and application, r Cg The SAA amino acid sequence is shown in SEQ ID NO. 1; the preparation method is to use primers P1 and P2 to... Cg The SAA coding region fragment was amplified by PCR, and the DNA sequences of primers P1 and P2 are shown in SEQ ID NO. 2 and SEQ ID NO. 3, respectively. The PCR amplification product was then mixed with the pET-30a vector via... Bam HI and Xhol After digestion with enzyme I, ligation was performed using T4 ligase, followed by transformation to construct recombinants; the recombinants were then transformed into *E. coli*. Transetta In the (DE3) expression strain, the expression strain was then cultured and IPTG was added to induce expression, followed by purification and refolding to obtain r. Cg SAA protein. The prepared r Cg SAA inhibits the growth of Vibrio splenti and Vibrio anguillarum under non-acidic conditions, and can also kill Vibrio splenti. It can be used to prepare drugs against Vibrio splenti and Vibrio anguillarum, and can be applied directly, effectively and safely to aquaculture.