Patents
Literature
46 results about "Polyploid" patented technology
Efficacy Topic
Property
Owner
Technical Advancement
Application Domain
Technology Topic
Technology Field Word
Patent Country/Region
Patent Type
Patent Status
Application Year
Inventor
Polyploidy is the state of a cell or organism having more than two paired (homologous) sets of chromosomes. Most species whose cells have nuclei (eukaryotes) are diploid, meaning they have two sets of chromosomes—one set inherited from each parent. However, some organisms are polyploid, and polyploidy is especially common in plants. In addition, polyploidy occurs in some tissues of animals that are otherwise diploid, such as human muscle tissues. This is known as endopolyploidy. Species whose cells do not have nuclei, that is, prokaryotes, may be polyploid, as seen in the large bacterium Epulopiscium fishelsoni. Hence ploidy is defined with respect to a cell. Most eukaryotes have diploid somatic cells, but produce haploid gametes (eggs and sperm) by meiosis. A monoploid has only one set of chromosomes, and the term is usually only applied to cells or organisms that are normally diploid. Males of bees and other Hymenoptera, for example, are monoploid. Unlike animals, plants and multicellular algae have life cycles with two alternating multicellular generations. The gametophyte generation is haploid, and produces gametes by mitosis, the sporophyte generation is diploid and produces spores by meiosis.
Method for cultivating polyploidy by inducing doubling of girdling calluses of branches and trunks of camellia oleifera
The invention relates to a method for inducing doubling cultivation of polyploidy by girdling calluses of camellia oleifera branches, the influence on tree vigor is reduced through girdling, the efficiency of regeneration buds is improved through thorough removal of cambium, the bottleneck in the prior art is overcome, chimera-free polyploidy material induction can be realized without in-vitro tissue culture, and the method is suitable for large-scale popularization and application. The method is simple and convenient to operate and low in cost, can effectively avoid the problems of rooting difficulty of tissue culture materials, transplanting loss of tissue culture seedlings and the like, provides a new technical approach for woody plant polyploid breeding, and has important popularization and application values.
Owner:CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
Genotyping of polyploids
The current invention pertains to a reliable method for determining the relative frequency of a sequence variant of interest in a nucleic acid sample derived from at least one polyploid cell, wherein the method uses a UMI to correct for any amplification biases. The invention further pertains to the use of a UMI for accurately determining the relative frequency of a sequence variant of interest in a nucleic acid sample derived from at least one polyploid cell.
Owner:KEYGENE NV
Citrus polyploidy efficient induction device
ActiveCN224267725UMultiple cultureMultiple growthPlant genotype modificationBiotechnologyCitrus volkameriana
The utility model belongs to the technical field of citrus cultivation, and particularly relates to a citrus polyploidy efficient induction device which comprises a machine body. A sealing door is rotationally arranged on the side wall of the machine body; the sealing doors are symmetrically arranged on the side wall of the machine body; an induction processor is mounted on the side wall of the machine body; the output end of the induction processor is connected with a built-in discharger; the built-in discharger is arranged on the inner side wall of the machine body; a spraying opening is formed in the side wall of the built-in discharger; the spraying openings are formed in the side wall of the built-in discharger in a linear array mode. A storage rack is fixedly connected to the interior of the machine body; the storage racks are symmetrically arranged in the machine body; an embedding groove is formed in the side wall of the storage rack; by means of the structure, tissue culture is not needed by adopting a living body induction technology, multi-time growth can be achieved from seed germination to regeneration, compared with a traditional in-vitro induction method, the method is more convenient, and multi-time cultivation of citrus can be achieved in a sealed state.
Owner:WEISHAN JUFENG AGRI TECH CO LTD
Method for carrying out nine-time breeding by utilizing female gametes
PendingCN121890504Areduce incidencehigh feasibilityPlant tissue cultureHorticulture methodsBiotechnologyGermplasm
The invention discloses a method for carrying out nine-fold breeding by utilizing female gametes, and relates to the technical field of biological breeding. Comprising the following steps: step 1, pollinating hexaploid persimmon pollen to a'rattan royal house 'containing 2n eggs; 2, collecting young fruits which are born for 65-70 days after pollination, stripping seeds, putting the seeds into a sterilized triangular flask, treating the seeds for 30 seconds in an ultra-clean workbench by using 70% ethanol, pouring out the seeds, adding a 1% NaClO solution, soaking the seeds for 10 minutes, and shaking the seeds once every 3-4 minutes. According to the method for carrying out nine-fold breeding by utilizing female gametes, in persimmon breeding, compared with a 2n pollen path, the 2n egg path is higher in feasibility, 2n eggs and complete male germplasm with good pollen traits are hybridized, pollination populations are expanded, and polyploidy seedless materials can be obtained.
Owner:HUAZHONG AGRI UNIV
Method for inducing polyploidy of radix peucedani by using fasciculate buds and application thereof
The application provides a method for inducing poly-podophylli rhizoma by using clump buds, comprising the following steps: S1, cutting stem segments from a podophylli rhizoma mother plant, soaking and cleaning to obtain explants; S2, disinfecting and cleaning the explants, inoculating into a clump bud induction medium to obtain podophylli rhizoma clump buds; S3, inoculating the clump buds into doubling liquid I, washing with a liquid clump bud induction medium after culture, and then transferring into doubling liquid II; S4, taking out the clump buds, cleaning, inoculating into a clump bud induction medium, and then inoculating into a bud proliferation medium; S5, waiting until 1-2 cm, screening out potential mutant strains through phenotypes, inoculating into the bud proliferation medium for subculture, and obtaining mutant plants; and S6, rooting culture of the mutant plants, and after root systems grow, planting and raising seedlings, and then poly-podophylli rhizoma plants are obtained. In the research, suitable concentration of colchicine is used for induction treatment twice, and through stage concentration switching and intermittent recovery strategies, the poly-ploid induction efficiency and explant survival are effectively considered.
Owner:HANJIANG NORMAL UNIV
A method for efficiently inducing heteropolyploid fry
This invention discloses a method for efficiently inducing allopolyploid fish fry, belonging to the field of fish breeding technology. The method of this invention includes the following technical steps: (1) Induction of spawning in distant hybridization parents: Selecting female and male parent fish from different subfamilies of Cyprinidae, and collecting mature eggs and semen respectively; (2) Obtaining hybrid fertilized eggs: Mixing the collected eggs with semen, and performing dry artificial insemination to obtain hybrid fertilized eggs; (3) Inducing allopolyploidy: Placing the fertilized eggs in a water bath for heat shock treatment to inhibit the expulsion of the second polar body or inhibit the first cleavage, and then quickly transferring the treated fertilized eggs to aquaculture water to continue hatching fry. The method of this invention combines the method of distant hybridization between subfamilies with the method of heat shock induction. After induction using this method, the multiplication rate of hatched fry can reach 100%, which significantly improves the yield of allopolyploid fish fry from distant hybridization.
Owner:HUNAN NORMAL UNIVERSITY
Chrysanthemum constitutive high expression promoter and application thereof in gene editing
The application discloses a chrysanthemum constitutive high-expression promoter and application thereof in gene editing. The nucleotide sequence of the promoter CmUbi is shown as SEQ ID NO. 4. Through double luciferase reporter experiments and stable genetic transformation experiments, it is proved that the expression activity of the CmUbi promoter in chrysanthemum protoplast and callus is significantly higher than that of commonly used CaMV 35S, AtRPS5a and corn ZmUbi promoters. A CRISPR / Cas9 gene editing vector is constructed by using the promoter, and a key gene of branch of hexaploid chrysanthemum is edited BRC1b , and a plurality of allele simultaneous mutation complete editing strains are successfully obtained, and the number of branches of the mutants is significantly increased. The endogenous high-activity promoter of the chrysanthemum provided by the application effectively solves the problems of low expression activity of an existing heterologous promoter in the chrysanthemum and poor gene editing efficiency of a polyploid, and provides an efficient and adaptive expression regulation element for chrysanthemum molecular breeding and functional genomics research.
Owner:NANJING AGRICULTURAL UNIVERSITY
Methods and compositions for the preparation and analysis of DNA libraries
PendingJP2026509614ANucleotide librariesMicrobiological testing/measurementA-DNANucleic acid sequencing
A method and composition for preparing a DNA library for replicating a target nucleic acid sequence are provided. A target DNA template containing the target sequence is circularized via a terminal adapter to form a circular construct, which is extended bidirectionally by polymerase-mediated elongation initiated at a nick site on the terminal adapter. Following polymerase-mediated elongation, a diploid DNA template is formed containing two copies of the target DNA template (and thus two copies of the target sequence). Each strand of the diploid DNA template contains a parent polynucleotide chain ligated to a newly synthesized daughter strand copy of the parent polynucleotide chain. Predetermined sequences, such as primer sequences, unique molecular identifiers, and sequence indices, can be included in the diploid DNA template. Sequencing of the diploid DNA template can reveal genetic / epigenetic information related to the target sequence. A method for constructing asymmetric and multiploid DNA template constructs is also provided.
Owner:F HOFFMANN LA ROCHE & CO AG
Genomic selection method and its application in breeding of homoeologous polyploid species
The present application relates to the technical field of bioinformatics, and particularly relates to a genome selection method and application thereof in breeding of homologous polyploids. The method comprises the following steps: constructing an additive genomic kinship matrix; constructing a genomic selection model based on the additive genomic kinship matrix; and estimating breeding values of individuals of a species according to the genomic selection model. The genotype matrix used in the additive genomic kinship matrix of the homologous polyploids is a polyploid genotype matrix, and the value of the genotype in the polyploid genotype matrix is determined by the copy number of any one allele. The present application proposes a genome selection method based on the homologous polyploid genomic kinship matrix for the species with the characteristics of homologous polyploids, which can accurately predict the genomic breeding values of individuals of the homologous polyploid species and improve the accuracy of genome selection, and has important significance in the field of genome selection breeding.
Owner:BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
Breeding method and identification method of triploid eucommia ulmoides
PendingCN121795316ALarge-scale ploidy identificationEarly stage of ploidy identificationPlant tissue cultureHorticulture methodsBiotechnologyColchicine
The invention relates to the technical field of eucommia ulmoides breeding. The invention provides a breeding method and an identification method of triploid eucommia ulmoides, which comprises the following steps: taking eucommia ulmoides branches, cutting, treating with colchicine solution, and culturing to obtain adventitious buds; cultivating the adventitious buds to obtain seedlings, performing ploidy identification on the seedlings, and screening out tetraploid plants; taking the tetraploid plant as a female parent, hybridizing with the diploid male parent, and harvesting triploid eucommia ulmoides seeds. The invention provides the method for efficiently and stably obtaining the homozygous tetraploid eucommia ulmoides clone, the problems of common chimeras and unstable materials in chemical induction are solved, and reliable parents are provided for cross breeding. A set of complete technical system for directionally creating the triploid eucommia ulmoides by taking tetraploid as a female parent and diploid as a male parent (reverse crossing) is established, and the technical blank from creation of a polyploid parent to breeding of a new triploid variety is filled.
Owner:RES INST OF NON TIMBER FORESTRY CHINESE ACAD OF FORESTRY
Bletilla striata autotetraploid induction and purification method based on steady-state pseudobulb
The invention relates to the technical field of traditional Chinese medicine propagation and quality improvement, and discloses a homeostatic pseudobulb-based bletilla striata autotetraploid induction and purification method, according to the research, a homeostatic subculture pseudobulb is treated by colchicine, an autotetraploid (2n = 4x = 64) is successfully induced, and the induction rate reaches 26.65%. A bud eye purification strategy is innovatively adopted, so that the chimera rate is reduced to 3.35%, and the ploidy stability is verified through flow cytometry and chromosome counting. Tetraploid plants show typical'canalization 'characteristics, and the pseudobulb multiplication coefficient is obviously higher than that of diploid plants. The established pseudobulb induction-bud eye purification-ploidy stabilization system provides an efficient normal form for polyploidy breeding of endangered medicinal orchid plants, and the tetraploid material has the characteristics of growth vigor enhancement and sustainable propagation and has great industrialization potential.
Owner:YUNNAN FENGHE SHULI BIOTECHNOLOGY CO LTD
Method for identifying the genetic relationship and ploidy of hybrid kelp polyploid
ActiveCN117844970BMicrobiological testing/measurementClimate change adaptationBiotechnologyElectrophoreses
The application discloses a method for identifying the genetic relationship and ploidy of hybrid kelp polyploids, which utilizes screened SSR markers to perform PCR amplification on the parents of a hybrid combination and the hybrid kelp polyploid offspring cultivated, and performs electrophoresis detection, so as to identify the genetic relationship of the hybrid kelp polyploids and realize the identification of the ploidy of the hybrid kelp polyploids. The screened kelp SSR markers need to meet the requirement that bands capable of distinguishing the parents of the hybrid combination exist after performing PCR amplification on the DNA of the parents of the hybrid combination and performing electrophoresis detection. The application is based on the molecular biology technology of kelp microsatellite molecular markers (SSR), and simultaneously identifies the genetic relationship of the hybrid combination, and realizes the purpose of accurately identifying the ploidy of the hybrid kelp polyploids from the molecular level.
Owner:SHANDONG ORIENTAL OCEAN SCI TECH +1
Application of H2A.Z1 protein and coding gene thereof in regulation and control of plant chromosome doubling
The invention discloses an H2A. Z1 protein and application of a coding gene of the H2A. Z1 protein in regulation and control of plant chromosome doubling. According to the invention, a model species poplar of a woody plant is taken as a research object, and a transgenic poplar with reduced H2A.Z1 gene expression quantity is constructed. Experiments prove that compared with wild poplar, the plant height and the stem node number of the transgenic poplar with reduced H2A.Z1 gene expression quantity are reduced, and the stem node length is increased. In addition, cells in stem and leaf organs of the transgenic poplar with reduced H2A.Z1 gene expression quantity become larger, cell chromosomes are doubled, and diploid is changed into tetraploid. The invention has important significance for researching polyploid breeding work of plants such as vegetables, important flowers, fruit trees and the like.
Owner:INST OF BOTANY CHINESE ACAD OF SCI
Method and system for correcting hi-c sequence alignment by fusing dna methylation information
The application discloses a method and system for correcting Hi-C sequence alignment by fusing DNA methylation information, and the method comprises the following steps: obtaining a preliminary alignment result of Hi-C sequencing of a sample genome relative to a reference genome and a methylation site map; obtaining a corresponding candidate alignment position and an original sequence alignment score of each read pair; positioning an alignment interval on the reference genome at both ends of each candidate alignment position; based on the methylation site map, counting the number of methylation sites covered at both ends of the candidate alignment position to obtain a methylation penalty score representing biological consistency; obtaining a recalibration comprehensive score based on the methylation penalty score, reordering all candidate alignment positions of the read pair, calculating an alignment quality update value of each candidate position, and outputting a corrected alignment result. The application solves the problem that in a polyploid and a highly repetitive genome, multiple alignment of Hi-C reads cannot accurately determine the real source position.
Owner:WUHAN FRASERGEN CO LTD
Method for efficiently inducing peony underground bud polyploidy and application thereof
The present application relates to the technical field of plant breeding, and discloses a method for efficiently inducing peony subterranean bud polyploidy and application thereof, comprising subterranean bud disinfection, cell synchronization, minimally invasive pretreatment, preparation of CS- Alg / PPy photothermal response slow-release microspheres, loading of composite inducer, microsphere implantation and photothermal regulation induction, polyploidy identification screening and transplanting, wherein the composite inducer comprises colchicine, 6-BA and graphene quantum dots, can block chromosome separation, realize cell chromosome doubling, regulate growth point cell division, simultaneously repair cell damage, improve bud survival rate, assist photothermal regulation to realize precise drug release, and promote expression of polyploid plant excellent traits. The method has high induction rate, high homozygosity and high bud survival rate, is suitable for cultivation of ornamental, cut flower, medicinal and stress-resistant peonies, and is advanced and practical in technology.
Owner:BOZHOU VOCATIONAL & TECHNICAL COLLEGE
Method for inducing litchi girdling callus and peripheral bud differentiation in field
The invention discloses a method for inducing litchi girdling callus and peripheral bud differentiation in a field, and relates to the technical field of plant propagation, the method comprises the following steps: cutting off the top of a trunk branch of a litchi growing vertically, and carrying out spiral girdling below the section of the top until the girdling depth reaches a wood layer; soaking absorbent cotton in a bud differentiation inducer to obtain an induction matrix; after the top section and the girdling opening of the litchi branch are covered with the induction matrix, bud differentiation treatment is conducted on the litchi branch; after bud differentiation treatment is completed, the litchi branches are subjected to moisture preservation and light shielding treatment, and adventitious buds are obtained. According to the method, wound induction is directly carried out on the litchi plants in the field, the browning problem in the in-vitro culture process is avoided by utilizing a plant self-repairing mechanism and combining with local medicament treatment, adventitious bud differentiation around girdling openings is promoted, a stable material source is provided for field screening of the polyploidy plants, the equipment and labor cost is reduced, and the method is suitable for large-scale popularization and application. Meanwhile, the survival rate of genetic variants is improved.
Owner:SOUTH SUBTROPICAL CROP RES INST CHINA ACAD OF TROPICAL AGRI SCI
Cultivation method of cork tree polyploidy
The invention relates to a cultivation method of cork tree polyploidy, and relates to the technical field of plant chromosome doubling. The method comprises the following steps: spraying an inducing solution to treat a top growing point of a balsa seedling at the seedling age of 15 days so as to induce chromosome doubling; wherein the inducing liquid comprises colchicine with the concentration of 0.1%-0.4% (w / v), and a preservative; 1 / 4 MS culture medium; 6-BA with the concentration of 3 mg / L; and a surfactant Silwet L-77 with a concentration of 0.1% (v / v), the spraying treatment is specifically as follows: an induction liquid is sprayed to the apical growth point once every 24 hours, spraying is carried out for three times in total, and the spraying amount each time enables the surface of the apical growth point to be uniformly wet and liquid drops do not drop. The method not only can effectively improve the polyploidy inductivity of balsa wood, but also can control a certain death rate of balsa wood seedlings, so that more polyploidy balsa wood plants can be obtained.
Owner:XISHUANGBANNA TROPICAL BOTANICAL GARDEN CHINESE ACAD OF SCI
Sequencing library construction method and application thereof in genome typing and high-density physical map construction
PendingCN121380288ANucleotide librariesMicrobiological testing/measurementGenomic SegmentGenomic sequencing
The invention discloses a sequencing library construction method and application of the sequencing library construction method in genome typing and high-density physical map construction. The method comprises the following steps: respectively connecting genome DNA fragments covering the whole genome of a target species to a cloning vector, then carrying out enzyme digestion by using restriction endonuclease, screening fragments of which the length is greater than the length of the cloning vector from the enzyme digestion fragments, and adding ligase to carry out catalytic connection to obtain a self-ligation product; carrying out PCR (Polymerase Chain Reaction) amplification by using the primer pair self-ligation product as a template, wherein an obtained PCR product is a genome sequencing library of the target species; in combination with a PacBio HiFi sequencing technology, the method provided by the invention omits the processes of BAC library construction and monoclonal selection, and the marker generation and genomic typing accuracy are significantly improved. The method can be applied to development of high-resolution bin markers of polyploidy species and genome assembly quality, and can promote breeding and improvement of polyploidy crops.
Owner:INSTITUTE OF VEGETABLES & FLOWERS CHINESE ACADEMY OF AGRICULTURAL SCIENCES +1
Method for inducing jellyfish polyploidy and application thereof
The application discloses a jellyfish polyploid induction method and application thereof, and belongs to the technical field of marine ornamental animal breeding. The jellyfish polyploid induction method takes the polyp stage of jellyfish as an induction object, and polyploid jellyfish is obtained by induction treatment through combination of colchicine and dimethyl sulfoxide. The method can obtain multiple polyploid new germplasms through one treatment under the use of a small amount of inducers. The obtained polyploid polyps can not only rapidly proliferate new individuals with polyploid characteristics through asexual reproduction, but also obtain jellyfish bodies with polyploid characteristics, such as fast growth speed, long life cycle, beautiful shape and large individual. Meanwhile, the method has the characteristics of simple operation and low cost. Therefore, the induction method has great application value and broad application prospect.
Owner:QINGDAO MARINE SCI & TECH MUSEUM (QINGDAO MARINE MUSEUM QINGDAO AQUARIUM)
Alfalfa low-lignin gene mutant and application thereof
The invention relates to the technical field of gene engineering, in particular to an alfalfa low-lignin gene mutant and application thereof. The medicago sativa low-lignin gene is a feruloyl acid-5-hydroxylase-1 protein mutant, and the amino acid sequence of the medicago sativa low-lignin gene is as shown in SEQ ID NO.1-3. According to the invention, efficient editing of four alleles coded by F5H1a is realized by using an independently developed efficient alfalfa gene editing carrier. According to the method disclosed by the invention, ferulic acid-5-hydroxylase-1 protein mutation in the polyploid alfalfa is induced, so that the content of total lignin in the polyploid alfalfa is reduced by 17%, the proportion of syringyl (S) units is remarkably reduced, the S / G ratio is reduced (about 60%), the quality of the f5h1a mutant relative to feed is increased by 34%, and the in-vitro dry matter digestibility in 30 hours is increased by 11%.
Owner:QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI +1
Methods of breeding guayule plants
PCT designated stageWO2026085445A1Plant tissue cultureHorticulture methodsBiotechnologyParthenium hysterophorus
Embodiments of the present disclosure are directed to breeding methods for guayule plants. The breeding methods disclosed herein generally include pollinating a maternal guayule plant with pollen from a paternal guayule plant to produce one or more progeny seeds. The maternal guayule plant is generally diploid and the paternal guayule plant is generally polyploid, optionally triploid. The progeny seeds are germinated to form a progeny population. Diploid progeny plants are selected from the progeny population and cultivated to produce a plant product.
Owner:BRIDGESTONE CORP +1
A method for creating a fertile tetraploid hybrid between an oryza sativa and an oryza australiensis genome
The application relates to a method for creating a fertile tetraploid hybrid between a cultivated rice and an Oryza australiensis genome, which comprises the following steps: using CX35-2X or YZ32-2X as a female parent material, and Oryza australiensis EE as a male parent material to perform hybridization, thereby obtaining first-generation hybrid seedlings; doubling the first-generation hybrid seedlings by colchicine, thereby obtaining hybrid tetraploid seeds. The application utilizes the whole genome transfer and polyploidization, that is, a distant hybrid containing a complete wild rice genome is obtained through distant hybridization, and then a hybrid allopolyploid is obtained through chromosome doubling, so that the excellent genes of the wild rice and the distant hybridization advantage of the polyploid are fully utilized.
Owner:HUBEI UNIV +1
Ulmus plant tissue culture medium and application thereof
The invention discloses an ulmus plant tissue culture medium and application thereof, and belongs to the technical field of tissue culture. The invention provides an ulmus plant tissue culture medium in order to solve the technical problems of low survival rate and poor seedling stress resistance of a general culture medium for woody plants in ulmus plant culture in the prior art. The culture medium is divided into a callus first-stage culture medium, a callus second-stage culture medium, a callus cell tillering culture medium, a bud and leaf growth culture medium and a root growth culture medium. According to the method, a synergistic scheme of berberine culture medium regulation and colchicine polyploidy induction is adopted, and the stress resistance of the ulmus tissue culture seedlings is remarkably improved through a dual mechanism of activating a stress resistance signal channel and inducing chromosome doubling, so that the problems that an existing general culture medium is poor in adaptability, and the tissue culture seedlings are vitrified and yellowed are solved; the efficient and stable factory rapid propagation of the ulmus plants is realized, and the method has important application value.
Owner:HEILONGJIANG DONGZE BIOTECHNOLOGY CO LTD
Polyploid induction and matched culture method of gentiana dahurica
The invention discloses a polyploidy induction and matched culture method for gentiana dahurica, which is characterized in that callus of gentiana dahurica is used as a material, and polyploidy induction is carried out by adopting a colchicine soaking and tissue culture combined method. The method comprises the following steps: selecting full gentiana dahurica seeds, disinfecting, inoculating to a primary culture medium, culturing, and soaking with a colchicine solution after callus is formed; inoculating the induced material into a proliferation culture medium for proliferation, and after adventitious buds are formed, transferring the adventitious buds into a rooting culture medium for rooting induction; polyploidy plants are identified through a chromosome counting method and subjected to acclimatization and transplantation, and a matrix with the ratio of peat soil to perlite being 3: 1 is adopted in a matched mode and appropriate temperature and humidity management is adopted.
Owner:GANSU MEDICAL COLLEGE
A haplotype-resolved assembly method and device for polyploid genomes
The application discloses a haplotype resolving assembly method and device for a polyploid genome. The application develops a haplotype resolving scaffold construction method integrating HiC data and an assembly graph to improve accuracy, integrity and continuity. By utilizing graph connectivity derived from long read overlaps, the key limitations of HiC are effectively overcome. When applied to complex polyploid genomes, the method of the application reduces sequence loss rate by up to tens of times, reduces sequence error rate by an order of magnitude, and improves the continuity of gapless sequences by several times compared with existing tools.
Owner:AGRI GENOMICS INST CHINESE ACADEMY OF AGRI SCI +1
Method for the production of polyploid megakaryocytes, and method for the production of IPSC-derived platelet-like particles using the same
PCT designated stageWO2026022217A1Blood/immune system cellsArtificially induced pluripotent cellsBiotechnologyPlatelet
The invention pertains to methods for the production of polyploid megakaryocytes using diploid megakaryocytes derived from induced pluripotent stem cells (iPSCs), the polyploid megakaryocytes so produced, and their use for the production of iPSC-derived platelet-like particles. The invention further pertains to the iPSCs-derived platelet-like particles produced using the polyploid megakaryocytes of the invention, and their use as a medicament.
Owner:INSTITUT GUSTAVE ROUSSY +2
Induction and cultivation method of paphiopedilum polyploidy
The invention provides an induction and cultivation method of paphiopedilum polyploidy. Comprising the following steps: enabling paphiopedilum seeds to germinate in a germination culture medium to form buds; the buds are soaked and induced in the dark for 6-36 h, and an induction culture medium is a 1 / 2 MS liquid culture medium containing colchicine with the mass fraction being 0.01-0.08%, dimethyl sulfoxide with the volume fraction being 2%, 2, 4-D with the concentration being 0.5-5 mg / L and TDZ with the concentration being 0.05-1 mg / L; carrying out protocorm-like body induction and proliferation on the mutated buds; carrying out differentiation culture on the protocorm-like body; carrying out rooting and strong seedling culture and transplanting; and tetraploid identification. According to the method, efficient induction of the paphiopedilum polyploidy is successfully realized by combining colchicine soaking induction with a protocorm-like body proliferation technology. A reliable polyploidy identification system is established, preliminary screening of plant characteristics and stomatal characters, ploidy analysis of flow cytometry and visual verification of chromosome tabletting are covered, and an important technology is provided for paphiopedilum breeding.
Owner:SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
Polyploid genome apparent data resolution analysis method
The invention provides a polyploidy genome apparent data resolution analysis method which comprises the following steps: S1, acquiring original data, removing linkers and filtering low-quality sequences; s2, carrying out single-ended comparison on the reference genome by using bowtie2 software and taking-k'n 'as a parameter, and screening comparison results; s3, recording double-end matched Reads and matched chromosome numbers; s4, dividing the reference genome into a plurality of sets, and independently constructing a reference genome index for each set of genome; s5, extracting sequences according to the records obtained in the step S3, and distributing the sequences to corresponding subgenomes according to chromosome numbers; and S6, comparing each group of sequences with corresponding sub-genomes according to a double-end comparison process, and finally identifying peaks information of each group of genomes, thereby completing the polyploidy genome apparent data resolution analysis. According to the method, by optimizing comparison parameters and adding a subsequent sequence identification screening strategy, apparent data between homologous chromosomes are accurately distinguished, and accurate distinguishing of genetic structures is achieved.
Owner:WUHAN FRASERGEN CO LTD
A method for breeding a new hybrid triploid oyster line with high growth rate and high resistance
The application discloses a breeding method of a hybrid triploid oyster new strain which is fast-growing and high-resistant. The hybrid triploid oyster new strain is obtained through the following technical links: breeding of a high-resistant and fast-growing diploid strain, establishment of a high-resistant and fast-growing tetraploid induction group strain, breeding of a high-resistant and fast-growing tetraploid new strain, breeding of a high-resistant and fast-growing hybrid diploid new strain, batch production of a high-resistant and fast-growing hybrid triploid new strain, and the like, combined use of population selection breeding, family selection breeding, stress breeding, hybrid breeding and polyploid breeding technologies. The hybrid triploid oyster new strain is fast-growing, high-resistant, adaptive to the habitat of North China sea area, poor in fertility, good in quality and high in fullness degree throughout the year, and can effectively solve the problems of high mortality, slow growth, high breeding risk and low benefit of Pacific oysters in North China sea area, and has remarkable popularization significance and value.
Owner:SOUTH CHINA SEA INST OF OCEANOLOGY CHINESE ACAD OF SCI +1
Method for inducing cane polyploid plant by using colchicine
The invention belongs to the technical field of plant polyploid breeding, and particularly relates to a method for inducing a cane polyploid plant by utilizing colchicine. According to the method, 0.03-0.04% of colchicine solution is used for inducing germinated cane seeds for 48-72 h, colchicine can induce cane chromosome doubling, plants are made huge, meanwhile, the growth speed is increased, on one hand, the yield of cane rattan is increased, the cultivation cost is reduced, the economic benefit is greatly improved, on the other hand, the soft quality of cane rattan can be improved, and the yield of cane rattan is increased. The method is used for producing different types of rattan products, and high-value utilization and product development of the cane rattan are achieved. The result of the embodiment shows that the induction rate of the polyploidy plant of the cane cane is as high as 6.36%-12.73%. Furthermore, the gibberellin solution is used for accelerating germination of the alpine cane seeds and then germinating the alpine cane seeds, the germination period of the alpine cane seeds can be greatly shortened, the germination rate and germination potential of the alpine cane seeds are improved, and seedlings emerge neatly.
Owner:ZHOUKOU NORMAL UNIV