7803results about "Plant tissue culture" patented technology

A novel soybean cultivar, designated 0491737, is disclosed. The invention relates to the seeds of soybean cultivar 0491737, to the plants of soybean 0491737 and to methods for producing a soybean plant produced by crossing the cultivar 0491737 with itself or another soybean variety. The invention further relates to transgenic soybean plants produced by transforming a soybean of the cultivar 0491737.

The immunoglobulins of the present invention are useful therapeutic immunoglobulins against mucosal pathogens such as S. mutans. The immunoglobulins contain a protection protein that protects the immunoglobulins in the mucosal environment. The invention also includes the greatly improved method of producing immunoglobulins in plants by producing the protection protein in the same cell as the other components of the immunoglobulins. The components of the immunoglobulin are assembled at a much improved efficiency. The method of the invention allows the assembly and high efficiency production of such complex molecules. The invention also contemplates the production of immunoglobulins containing protection proteins in a variety of cells, including plant cells, that can be selected for useful additional properties. The use of immunoglobulins containing protection proteins as therapeutic antibodies against mucosal and other pathogens is also contemplated.

The invention provides compositions comprising polynucleotide molecules encoding certain pesticidal polypeptides which exhibit plant parasitic nematode and/or insect control properties, and are particularly directed to controlling plant parasitic pest species of nematodes and insects known to infest crop plant species. Methods for controlling pests are disclosed in which the toxic proteins are provided in the diet of the targeted plant pests. The invention also provides compositions such as nucleic acids, proteins, and plant and bacterial cells, plants, and seeds containing the nucleic acid and protein compositions, as well as methods and kits for identifying, detecting, and isolating the compositions of the present invention. The invention further provides a method of producing crops from recombinant seeds which contain the polynucleotide molecules encoding the pesticidal polypeptides of the present invention.

A process for integrating a DNA fragment into the genome of a cell of a monocotyledonous plant, the process comprising the steps of: 1) incubating, prior to contacting with the DNA fragment, a culture of untransformed monocotyledonous plant cells on a medium comprising a plant phenolic compound, for a period of time sufficient to stimulate cell division and enhance competence for integration of foreign DNA; and 2) contacting the untransformed cells with the DNA fragment under conditions in which the DNA fragment is taken up by the untransformed cells and is stably integrated in the genome of the untransformed cells, to generate transformed cells.

Compositions and methods are disclosed for automated storing, tracking, retrieving and analyzing biological samples, including dry storage at ambient temperatures of nucleic acids, proteins (including enzymes), and cells using a dissolvable dry storage matrix that permits recovery of biologically active materials. RFID-tagged biological sample storage devices featuring dissolvable or dissociable matrices are described for use as supports of biological samples, which matrices can be dried and subsequently rehydrated for sample recovery. Also disclosed are computer-implemented systems and methods for managing sample data.

This invention relates to corn (Zea mays L.) seed and grain having a significantly higher oleic acid content than conventional corn by virtue of heritable genes for increased oil and oleic acid content and/or lowered levels of linoleic acid. The present invention also relates to the production of high oil, high oleic grain, its oil, its progeny and its use.

The immunoglobulins of the present invention are useful therapeutic immunoglobulins against mucosal pathogens such as S. mutans. The immunoglobulins contain a protection protein that protects the immunoglobulins in the mucosal environment.The invention also includes the greatly improved method of producing immunoglobulins in plants by producing the protection protein in the same cell as the other components of the immunoglobulins. The components of the immunoglobulin are assembled at a much improved efficiency. The method of the invention allows the assembly and high efficiency production of such complex molecules.The invention also contemplates the production of immunoglobulins containing protection proteins in a variety of cells, including plant cells, that can be selected for useful additional properties. The use of immunoglobulins containing protection proteins as therapeutic antibodies against mucosal and other pathogens is also contemplated.

A lettuce cultivar, designated 21-0406127-B, is disclosed. The invention relates to the seeds of lettuce cultivar 21-0406127-B, to the plants of lettuce cultivar 21-0406127-B and to methods for producing a lettuce plant by crossing the cultivar 21-0406127-B with itself or another lettuce cultivar. The invention further relates to methods for producing a lettuce plant containing in its genetic material one or more transgenes and to the transgenic lettuce plants and plant parts produced by those methods. This invention also relates to lettuce cultivars or breeding cultivars and plant parts derived from lettuce cultivar 21-0406127-B, to methods for producing other lettuce cultivars, lines or plant parts derived from lettuce cultivar 21-0406127-B and to the lettuce plants, varieties, and their parts derived from the use of those methods. The invention further relates to hybrid lettuce seeds, plants, and plant parts produced by crossing cultivar 21-0406127-B with another lettuce cultivar.

Rice plants are disclosed with two separate, but synergistic mechanisms for resistance to herbicides that normally inhibit a plant's acetohydroxyacid synthase (AHAS) enzyme. The herbicide resistance of plants with both resistance mechanisms is substantially greater than one would expect from a simple combination of the two types of resistance. The first of the two resistance mechanisms is a metabolic pathway that is not fully understood, but that does not itself involve a mutant AHAS enzyme. The second resistance mechanism is a mutant AHAS enzyme, an enzyme that shows direct resistance to levels of herbicide that normally inhibit the enzyme, in both in vivo and in vitro assays. Besides controlling red rice, many AHAS-inhibiting herbicides also effectively control other weeds that are common in rice fields. Several of these herbicides have residual activity, so that a treatment controls both existing weeds as well as weeds that sprout later. No herbicide currently available for use on rice has residual activity against a broad spectrum of weeds including red rice. With effective residual activity against red rice and other weeds, rice producers now have a weed control system superior to those currently used.

An automated seed sampler includes a sampling station; a sampler for removing material from a seed in the sampling station; a seed conveyer for conveying the seed from the sampling station to a compartment in a seed tray; and a conveyor for conveying the material removed from the seed to a corresponding compartment in a sample tray. The method of the present invention comprises feeding seeds individually to a sampling station, removing a sample from the seed in the sampling station; conveying the sample to a compartment in a sample tray, and conveying the seed to a corresponding compartment in a seed tray. The samples can be tested, and the seeds can be sorted according to the results of the testing of their corresponding samples.

A lettuce cultivar, designated ‘Blade’, is disclosed. The invention relates to the seeds of lettuce cultivar ‘Blade’, to the plants of lettuce cultivar ‘Blade’ and to methods for producing a lettuce plant by crossing the cultivar ‘Blade’ with itself or another lettuce cultivar. The invention further relates to methods for producing a lettuce plant containing in its genetic material one or more transgenes and to the transgenic lettuce plants and plant parts produced by those methods. This invention also relates to lettuce cultivars or breeding cultivars and plant parts derived from lettuce cultivar ‘Blade’, to methods for producing other lettuce cultivars, lines or plant parts derived from lettuce cultivar ‘Blade’ and to the lettuce plants, varieties, and their parts derived from the use of those methods. The invention further relates to hybrid lettuce seeds, plants, and plant parts produced by crossing cultivar ‘Blade’ with another lettuce cultivar.

An automated seed sampler includes a sampling station; a sampler for removing material from a seed in the sampling station; a seed conveyer for conveying the seed from the sampling station to a compartment in a seed tray; and a conveyor for conveying the material removed from the seed to a corresponding compartment in a sample tray. The method of the present invention comprises feeding seeds individually to a sampling station, removing a sample from the seed in the sampling station; conveying the sample to a compartment in a sample tray, and conveying the seed to a corresponding compartment in a seed tray. The samples can be tested, and the seeds can be sorted according to the results of the testing of their corresponding samples.

This invention provides method and devices for receiving and/or delivering liquid to an object, devices for delivering liquid to a plant/seed/growing medium comprising: a conduit comprising: at least two about parallel plates one or more walls substantially sealing the plates and forming a substantially enclosed space, such that the devices are useful in hydroponic gardens. This invention also provides hydroponic gardens with such devices. The parallel plates and the walls can be rigid and hydrophobic.

The present invention relates to a device and method for analyzing agricultural products. More particularly, the present invention relates to a device and method for rapid, non-destructive analysis of the physical and chemical characteristics of one or more seeds.

Plants, plant parts and plant seeds that are resistant to imidazolinone herbicides are provided. Plants are disclosed that contain a mutation in an AHAS gene. Specifically, plants are disclosed that contain a mutant AHAS gene allele of the Brassica juncea B genome. Two B. juncea AHAS gene sequences (BjAHAS-a and BjAHAS-b) and one B. nigra AHAS gene sequence (BngrAHAS) are disclosed. The sequence of the mutant allele, BjAHAS-bR, is also disclosed. Various methods are disclosed that include creation of mutant B. juncea lines, selection for herbicide resistant lines and determining the presence of the BjAHAS-bR mutant allele after crosses.

The invention discloses a breeding method for aseptic seeding and test tube seedling formation of orchids and the adopted broad-spectrum culture medium. In this method, a healthy orchid mother plant is selected, and artificially pollinated when it blooms, and the fruit that develops to maturity after pollination and has not cracked is used as an explant, or the orchid fruit that is basically mature and not cracked is taken from the wild as an explant. Implants undergo reproductive steps such as aseptic sowing, seed germination, strong seedling cultivation, and test-tube seedling transplantation. The specially prepared medium is used for aseptic sowing and seed germination to obtain small plants, and then the small plants are cultivated on the specially prepared strong seedling medium, and the seedlings are hardened under natural light in the greenhouse for 7-14 hours before being released from the bottle. One day, the test-tube seedlings were taken out, the root medium was washed off, and they were planted in a mixed substrate of bark, bluestone and peat, and further cultivated into seedlings. The invention has the characteristics of high seed germination rate, fast seedling growth, good seedling quality, low cost, etc., can obtain a large number of test-tube seedlings in a short period of time, and the transplanting survival rate of the seedlings can be kept above 90%. An effective way can be provided for the production of orchid seedlings.

This invention describes a simple method useful for the excision and isolation of maize immature embryos. The embryos are useful for plant tissue culture and transformation methods.

An assembly for promoting the growth of plant tissues that includes a plurality of plates each defining an array of wells wherein each of the wells contains a tissue sample. Support for the plates is provided by a rack having a plurality of vertically stacked shelves that may include one or more register depressions that urge the plates into predetermined positions. Light for the tissue samples is provided by a plurality of light-emitting diode arrays each mounted on a circuit board. Each circuit board is supported by a respective card edge connector of the rack so that the light-emitting diodes are in proximity to the plates supported on one of the shelves therebelow. Preferably, the light-emitting diode array corresponds to the well array supported in the registered position on the shelf therebelow so that each light-emitting diode is centered above a respective one of the wells.

The present invention relates to excision of explant material comprising meristematic tissue from cotton seeds. Methods for tissue preparation, storage, transformation, and selection or identification of transformed plants are disclosed, as are transformable meristem tissues and plants produced by such methods, and apparati for tissue preparation.

A treatment method for wastewater employing sulfur dioxide and lime chemical dewatering technology in conjunction with an environmental photo biomass/algae biological treatment system growing photo biomass/algae to reduce dissolved solids, heavy metals, and ammonia in the wastewater to produce recovered treated wastewater for vegetation consumption, biofuel feedstock, and biofuel and carbon credits.

Production of recombinant human serum albumin with rice albuminous cell as biological reactor is carried out by taking rice albuminous cell protein as storage site for recombinant protein, taking rice albuminous specific expression starter and signal peptide, entering inductive recombinant human serum albumin into inner-film system of rice serum albumin cell and storing it in rice albuminous protein. It is cheap, has more yield and higher expression.

The invention discloses a method for tissue-culture breeding and wild-simulated cultivation of dendrobium candidum, which comprises: inoculating dendrobium candidum tender straw slices onto a culturemedium for culture under conditions of a temperature of 22 to 24 DEG C and an illuminance of 1,000 to 1,500 Lx to first induce the growth of calles to form protocorms and then promote the multiplicative growth of the protocorms into 5 to 8 centimeter strong seedlings; and moving the seedlings out of a culture room, hardening the seedlings at a normal temperature for 3 to 5 days and transplanting the seedlings into the ground in a green house. The plane basic layer of a cultivation check field in the green house are provided with spaced breather holes; the upper layer of the cultivation check field is a cultivation medium; the seedlings are planted at a row spacing of 12*8cm and a spacing in the row of 12*8cm; in the green house, the temperature is 0 to 28 DEG C, the humidity is 60 to 70 percent and the shade density is 70 to 80 percent; and disease and pest prevention and control are carried out periodically and the dendrobium candidum can be harvested in 3 to 4 years. The method improves the civil year breeding coefficient of the dendrobium candidum by about 10 times, provides enough seedlings for large-scale cultivation, solves the problem of wild-simulated cultivation and realizes scale cultivation.

A process of recommending crop varieties based on management categories. The management categories are determined by utilizing indices, which measure the economic implication of physical and chemical properties of a specific soil type in a region. The management categories may then be indicated graphically by indicia overlaying digitized soil maps, each of the indicia grouping soil types within a management category. Each crop variety is characterized by how the crop variety performs in each of the management categories. Each of the crop varieties may then be assigned an indicium of the management category for which it is adapted.

The invention discloses a method of hardwood cutting propagation of Qilian Juniper, which is characterized in that, annual hardwood of the Qilian Juniper having the age ranges from seven years to ten years is cut as branches; the average length of the branches ranges from 14.0cm to 18.0cm; the average diameter of the branches ranges from 3.0mm to 5.0mm; on the last ten days of April of each year, the branches are inserted into a greenhouse for seedling; the cutting medium is the mixture of peat and perlite mixed according to the proportion of 1:1; the cutting medium is firstly formed into a medium sausage having the length ranging from 13cm to 16cm; then the cutting medium is put in a 58 x 32cm tray and is put on the cutting bed in the greenhouse. The breeding of Qilian Juniper is realized by the method of hardwood cutting propagation. The method has the advantages of reduced seedling period of Qilian Juniper, saved cost and remarkable effect.

An apparatus, system and method for removing and treating contaminated materials on a bottom of a body of water and introducing growth packets to revitalize the treated bottom of the body of water. The structure may comprise a vessel with an open face. The vessel may be lowered down to the bottom of the body of water with the face facing down. As a result, the vessel and the bottom form an isolated space. The structure may comprise at least one agitating device(s) for stirring up the materials inside the vessel so as to form a mixture containing the sediment materials which in turn contain the contaminants. Multiple at least one pipe(s) may be coupled to the vessel for transporting the mixture out of the vessel for processing (filtering, treating with chemicals, etc.) so as to neutralize or eliminate the contaminants in the mixture. Then, the treated mixture can be returned to the inside of the vessel via the at least one pipe(s).

The present invention discloses and claims methods and devices for the rapid mechanical isolation of monocot plant tissues suitable for transformation or tissue culture. The invention includes mechanical devices for substantially isolating target plant tissues for use as transformable explants, and propagation of transgenic plants and plant tissues.

The present invention provides methods and devices for the rapid isolation of monocot plant embryos suitable for transformation or tissue culture. The invention includes mechanical devices for substantially isolating plant embryos for use as transformable explants. Media suitable for isolating plant embryos and methods for their preparation are also provided.

Multiple shoot structures are induced from plant tissues (e.g., shoot apices or axillary buds on an artificial medium) to produce multiple shoot cultures. These multi-shoot cultures are then transformed by known transformation methods. Plants are subsequently regenerated from the transformed cells. Crops that may be efficiently transformed by this method include plants normally recalcitrant to transformation such as sugar beet, sunflower, soybean, cotton, tobacco, tomato, peanuts, melons, watermelon, squash, Brassica, and pepper.

The invention discloses a hormone-free tissue culture method for dendrobium candidum, which comprises four tissue culture stages including germinating of seeds of the dendrobium candidum into spherical stems, multiplication and differentiation, and rootage and sound seedlings at stem sections; culture media used at the four stages include a hormone-free MS (Murashige and Skoog) culture medium, a hormone-free MS basal culture medium plus 15-20 g/l mashed potato culture medium, a hormone-free MS basal culture medium plus 15-20 g/l mashed banana plus 15-20 g/l mashed potato culture medium and a hormone-free 1/2 MS basal culture medium plus 15-20 g/l mashed banana plus 15-20 g/l mashed potato culture medium. The polysaccharide content and total alkaloid content in the culture of the dendrobium candidum obtained by hormone-free culture are increased compared with hormone-containing culture; the growth state of the dendrobium candidum is normal; the genetic diversity of the plant variety is protected; with the adoption of the method, the tissue culture of the dendrobium candidum is more convenient, green and environmentally friendly, and the resources are saved.

The invention discloses a method for cultivating algam Dendrobium nobile in group in water. Since the blades of algam Dendrobium nobile before output bottle will not form complete horny layer to process effective photosynthesis, according to the growth and physiological characters, the algam Dendrobium nobile output bottle is cultivated by water cultivate device, to be cultivated for 30 days at right condition, therefore, cultivated seeds can be transplanted into large field. The invention can effectively resolve the problems as low survival rate and improve survival rate to 95%.