2401results about "Plant tissue culture" patented technology

Systems for plant culture include a chamber featuring one or more walls enclosing a spatial volume internal to the chamber, where the one or more walls include a surface for supporting a plant within the enclosed spatial volume, a gas delivery apparatus with at least one gas source, a nutrient delivery apparatus with a reservoir, a sampling apparatus connected to a port formed in the one or more walls, and a controller configured so that during operation of the system, the controller activates the nutrient delivery apparatus to deliver an aqueous growth medium to the plant, and activates the gas delivery apparatus to deliver into the enclosed spatial volume a mixture of isotopically-substituted gases. Also provided are methods of use of the system for measuring nitrogen in a plant and for identifying microbes capable of providing fixed nitrogen to a plant.

The invention discloses a sugarcane germplasm resource evaluation and breeding method for smart agriculture. The sugarcane germplasm resource evaluation and breeding method comprises the following steps: step 1, collecting materials with wide genetic diversity; establishing an intelligent incubator and a greenhouse, and monitoring environmental parameters by using an Internet of Things sensor; 2, performing data acquisition on the related germplasm resources by using an unmanned aerial vehicle, a robot and a near infrared spectrum technology, and constructing a phenotype database for data management; meanwhile, genotype identification is carried out by combining molecular marker-assisted selection and genome selection technologies, molecular markers of key genes are mined, and the genetic value of related germplasm is evaluated; step 3, constructing a hybrid combination prediction model by using an AI algorithm, optimizing parent matching of the related germplasm and the cultivated sugarcane, monitoring and collecting the filial generation at the same time, and analyzing and screening in combination with smart agriculture; 4, demonstration planting is conducted on the bred excellent sugarcane strain, and application of the excellent strain is improved through government-enterprise-scientific research institution collaborative popularization and in combination with farmer technical training.

Methods, including high-throughput, automated methods for preparing, by mechanical and / or robotic means, monocot leaf explants for transformation, are provided.

The invention relates to the technical field of plant tissue culture, in particular to a Rhododendron pulchrum tissue culture method based on callus regeneration. According to the method, the tender cotyledons of aseptic seedlings are taken as explants, and an efficient Rhododendron pulchrum tissue culture and rapid propagation technical system is established through induction of calluses, subculture multiplication of the calluses, differentiation of adventitious buds, bud strengthening and rooting culture, so that complete tissue culture progeny plants are formed. The induction rate of the callus tissue of the system is 100.0%, the proliferation multiple of the callus tissue in a subculture cycle reaches 5.2 times, 28.5 adventitious buds can be differentiated from a callus tissue block with the size of about 0.5 mm * 0.5 mm * 0.5 mm, the rooting rate reaches 95.8%, the system is not limited by factors such as seasons, climate and regions, and efficient and rapid propagation can be achieved all the year round. Meanwhile, the establishment of the system also lays a technical foundation for genetic transformation improvement, mutant screening breeding and the like of the Rhododendron pulchrum based on the callus.

The invention relates to a high-frequency regeneration and rapid propagation method of Enkianthus chinensis tissue culture seedlings. The invention is characterized in that dormant axillary buds of semi-lignified young stems are used as the base. The method comprises the following main steps: sampling and disinfection treatment of explants, inducing of dormant axillary bud germination, inducing of adventitious bud and lateral bud high-frequency generation, strong seedling culture, test-tube rooting culture and acclimatization and transplanting. Finally, regenerative plants which have consistent parental character, excellent quality and strong stress resistance are obtained. According to the invention, lots of seedlings with consistent specification and excellent quality can be obtained within a short period of time, and marketization progress of Enkianthus chinensis is promoted powerfully.

The invention relates to the technical field of plant genetic engineering, in particular to a method for efficiently and rapidly obtaining a stable transgenic jujube plant based on agrobacterium rhizogenes. The invention provides a method for efficiently and rapidly obtaining a stable transgenic jujube plant based on agrobacterium rhizogenes, which comprises the following steps: by taking a leaf as an explant, infecting a leaf disc by using the agrobacterium rhizogenes to directly obtain a transgenic leaf, inducing the generation of adventitious buds of the leaf, and culturing the adventitious buds to obtain the transgenic plant. The method disclosed by the invention is simple to operate and shorter in period, and the obtained transgenic plant is good in repeatability and high in transformation efficiency. The research on the agrobacterium rhizogenes-mediated genetic transformation of the jujube has important significance. By continuously optimizing the technology and the research method, the genetic transformation efficiency and the stability of the jujubes are expected to be improved, and a firmer foundation is provided for application in the fields of rapid genetic improvement, functional gene research and the like of the jujubes.

The invention discloses a large-scale preparation method of ginseng exosomes, which comprises the following steps: culturing calluses: inoculating explants into an induction culture medium until ginseng calluses are generated; proliferation of the calluses: inoculating the newborn calluses into a proliferation culture medium for proliferation culture; inducing germination and rooting: after the ginseng callus is amplified, transplanting the ginseng callus into a germination induction culture medium until the ginseng callus is germinated, and then transferring the ginseng callus into a rooting culture medium for culturing and rooting; and preparing the exosome, namely grinding root hairs grown from the ginseng callus regeneration plant, uniformly mixing the ground root hairs with PBS (Phosphate Buffer Solution) or performing liquid culture on ginseng suspension cells, and finally performing centrifugal treatment to obtain the ginseng exosome. According to the large-scale preparation method of the ginseng exosome, the endotoxin pollution in the growth and development process is reduced through ginseng callus culture rooting, and then the ginseng exosome is prepared, so that the large-scale preparation requirement in modern production and processing is met.

The invention relates to the technical field of plant stress resistance screening, in particular to a method for screening drought-resistant mutants after EMS mutagenesis of bamboo reed cluster buds, which comprises the following steps: S1, performing hydroponic seedling strengthening on wild tissue culture seedlings and EMS-treated rooting tissue culture seedlings to obtain wild bamboo reed seedlings and EMS-treated bamboo reed seedlings; s2, screening suitable concentration of PEG-6000: screening wild type bamboo reed seedlings by using a series of PEG-6000 solutions with different concentrations, comparing survival rates of the bamboo reed seedlings under different treatments, and screening suitable concentration for simulating drought stress by a PEG-6000 water culture method; s3, simulating drought stress conditions by using the screened PEG-6000 solution with proper concentration as a permeable medium, and screening the EMS treated bamboo reed seedlings to obtain drought-resistant plants. The screening method provided by the invention is not limited by seasons, has high accuracy, and is a simple, convenient and rapid method for screening drought-resistant bamboo reed plants.

The embodiment of the invention provides a method for regulating and controlling the form of ginseng adventitious roots and saponin accumulation. The method comprises the following steps: (a) carrying out mutagenesis treatment on initial ginseng adventitious roots by using ethyl methane sulfonate (EMS); (b) performing multiple rounds of screening and subculture to obtain to-be-selected ginseng adventitious roots with different forms and saponin accumulation; and (c) determining one or more target genes for regulating and controlling the form of the ginseng adventitious root and saponin accumulation based on the ginseng adventitious root to be selected.

The invention relates to the technical field of plant tissue culture, and provides a method for improving the embryo emergence rate of free microspores of non-heading Chinese cabbage by using 5-AzaC, and in the microspore culture stage, 5-AzaC with the concentration of 0.005-0.020 mg is added into a microspore culture medium. The invention designs a method for increasing the embryogenesis rate of free microspores of the non-heading Chinese cabbage by adding 5-AzaC into a microspore culture medium of the non-heading Chinese cabbage. According to the method, the embryogenesis rate of the free microspores of the non-heading Chinese cabbage can be remarkably improved, a non-heading Chinese cabbage microspore culture system is more easily established, the haploid breeding efficiency is improved, and the breeding process is accelerated.

The invention discloses a tissue culture method of a double-petal hemerocallis fulva scapes, which comprises the following steps: taking a double-petal hemerocallis fulva scapes, taking a growing point as a center, transversely cutting into long sections which are not less than 5cm, washing and disinfecting; putting the disinfected long segments into enzymatic hydrolysate for cell wall enzymolysis, taking out the long segments after enzymolysis is completed, and washing the long segments with sterile water; transversely cutting the long section subjected to enzymolysis into small sections of 1-2cm by taking the growing point as the center; and carrying out tissue culture by taking the small sections as explants. The method applies the double-petal hemerocallis fulva variety, and has the advantages that materials can be taken at any time in the flowering phase, the tissue culture efficiency is high, and the quality is good.

The invention relates to the technical field of tissue culture and microorganisms, in particular to a seedling culture method for improving disease resistance of crops by inoculating disease-preventing and growth-promoting bacteria, which comprises the following steps: step 1, culturing tissue culture seedlings to be inoculated with the disease-preventing and growth-promoting bacteria in a tissue culture manner; step 2, inoculating disease-preventing growth-promoting bacteria at the roots of the tissue culture seedlings; step 3, carrying out spot check to determine that the disease-preventing growth-promoting bacteria are successfully negatively colonized in the tissue culture seedling body, if colonization is successful, entering step 5, and if colonization is not successful, entering step 4 and then entering step 5; 4, when the disease-preventing growth-promoting bacteria are inoculated in the step 2, auxiliary bacteria are synchronously inoculated; 5, domesticating and transplanting the tissue culture seedlings successfully colonized with the disease-preventing growth-promoting bacteria; according to the method, disease-preventing growth-promoting bacteria can be colonized into tissue culture seedling bodies in advance, so that pathogenic bacteria entering crop bodies in the later period are reduced, and the purpose of disease-preventing seedling raising is achieved.

The invention discloses a tissue culture method of a citral type cinnamomum bodinieri stem with an axillary bud, and relates to the technical field of forest tree tissue culture. The tissue culture method comprises the following steps: selecting a current-year semi-lignified stem on a citral type cinnamomum bodinieri excellent single plant for disinfection treatment, and inoculating the stem on an axillary bud primary induction culture medium for axillary bud primary induction culture; after stem axillary buds in the axillary bud primary induction culture medium germinate, stem segments with axillary buds are soaked with a phycocyanobilin solution and then inoculated into the adventitious bud induction culture medium, red light irradiation treatment is conducted, conventional adventitious bud induction culture is conducted after red light irradiation, adventitious bud subculture multiplication culture, strong seedling culture and rooting induction culture are conducted, and the stem segments with the axillary buds are obtained. And finally, hardening seedlings and transplanting. According to the tissue culture method provided by the invention, the adventitious bud multiplication coefficient can reach more than 5, the rooting rate reaches up to 90.00%, the average rooting number also reaches 3.44, and the survival rate after two months is more than 85%. The invention provides support for better development and utilization of citral type Cinnamomum bodinieri, and has important significance for popularization and application of citral type Cinnamomum bodinieri.

The invention discloses a salt-tolerant reed seedling and a cultivation method thereof. The method comprises the following steps: seed selection and pretreatment; performing exogenous induction treatment; performing germination culture; salt gradient domestication culture; salt tolerance screening, seedling hardening and transplanting. According to the invention, by combining exogenous induction and salt gradient domestication culture, the stress resistance of the plant is regulated, and the salt tolerance mechanism in the seed is activated, so that a foundation is laid for better adaptation and growth in a high-salt environment subsequently; the salt-tolerant reed seedlings induced and domesticated by the method are high in seedling rate, the survival rate of acclimatization and transplantation is 90% or above, the breeding cycle is shortened to be within 100 days, the cultivation cycle is short, and the method is suitable for large-scale popularization and application.

The present disclosure provides methods of gene transformation of sunflower based on Agrobacterium-mediated gene delivery to meristem cells of fresh imbibed / germinated sunflower seeds. One embodiment of the present disclosure is a method of directly transforming a sunflower from a mature seed with a heterologous polynucleotide, comprising: generating an explant from a germinated sunflower seed by removing cotyledons and primary leaves having intact meristem and tender shoot radicles; contacting the sunflower explant comprising the cotyledon with Agrobacterium comprising a heterologous polynucleotide in an infection medium supplemented with a plant hormone using vacuum and ultrasound treatment; co-culturing the explant from a germinating sunflower plant in a culture medium lacking the plant hormone in the presence of the Agrobacterium genus; culturing the mature seed explant in a regeneration medium comprising a selective agent; and culturing the seed with the regenerated tender shoots and with the secondary roots in a culture medium lacking the selective agent.

The invention discloses a strawberry seedling and tissue culture and non-test tube rooting combined rapid propagation method, which comprises the following steps: selecting an explant, and disinfecting; inoculating and culturing; performing non-test-tube rooting culture; and hardening seedlings and transplanting. Through non-test-tube rooting culture, the process is simplified, the pollution risk is reduced, the transplanting difficulty of tissue culture seedlings is reduced, and the survival rate is increased; through the combination of tissue culture and a non-test-tube rooting technology, the survival rate of acclimatization and transplantation is as high as 91% or above; the method realizes efficient and low-cost propagation of strawberries, and is suitable for large-scale popularization and application.

The invention relates to the technical field of agricultural breeding, in particular to a bamboo reed saline-alkaline resistant screening and stress-resistant tissue culture seedling raising method which comprises the following steps: step 1, pretreatment; step 2, dynamic salt stress screening: placing the seed stems in a mixed salt-alkali solution with gradient increasing, culturing, measuring the bud length and root length growth rate of axillary buds of the seed stems, fitting a salt concentration-growth rate curve, and extracting a semi-inhibitory concentration IC50 and a maximum tolerance concentration MTC; step 3, salt tolerance grade classification; 4, carrying out stress-resistant tissue culture seedling culture: carrying out in-vitro culture on the axillary bud explant of the high-salt-resistant variety in a culture medium, and inducing clumpy buds and rooting in combination with a salt-resistant inducer; 5, domestication and transplanting: performing gradual salt stress domestication on the tissue culture seedlings, transplanting the tissue culture seedlings into a saline-alkaline environment, and monitoring the adaptability of the tissue culture seedlings in the saline-alkaline environment. According to the method disclosed by the invention, the efficient cultivation system of the suitable-to-grow bamboo reed seedlings in the saline-alkali soil is established by fusing dynamic stress response analysis and stress resistance phenotype directional strengthening technologies.

The invention relates to the technical field of plant tissue culture seedling raising, in particular to paenibacillus feed and application thereof in promoting cunninghamia lanceolata test-tube plantlet height growth. The feed paenibacillus paenibacillus SY0317 is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC NO: 20575, the preservation address is Institute of Microbiology, Chinese Academy of Sciences, No.3, No.1 Yard, Beichen West Road, Chaoyang District, Beijing, and the preservation time is August 31, 2020. The cunninghamia lanceolata test-tube plantlet cultured by adopting the strain has high growth speed, the growth amount is increased by more than 60%, the culture time of the cunninghamia lanceolata tissue culture plantlet is shortened, large-scale production of the cunninghamia lanceolata tissue culture plantlet can be realized, and the strain has remarkable economic and social benefits.

The invention discloses a dendrobium officinale tissue culture and rapid propagation seedling raising method, which belongs to the field of biotechnology, and comprises the following steps: S1, explant treatment: selecting a dendrobium officinale plant with good growth vigor and no obvious pest and disease damage, carrying out high temperature stress treatment at 40-45 DEG C for 6-8 days, then taking the tender stem of the dendrobium officinale plant subjected to high temperature stress treatment as the explant, and carrying out tissue culture and rapid propagation on the explant; directly transferring the explant to a low temperature of 2-6 DEG C for stress treatment for 8-12 hours; s2, callus induction: taking the explant treated in the step S1, disinfecting the explant, taking the explant without axillary buds, cutting the explant into small blocks, inoculating the small blocks to a callus induction culture medium, and carrying out dark culture for 12-15 days; the callus induction culture medium comprises 1.5 to 3 mg / L of MS + 2, 4-D, 2 to 3 mg / L of KT and 0.1 to 0.3 mg / L of NAA; s3, bud differentiation induction and rooting induction. According to the tissue culture and rapid propagation method for the dendrobium officinale, the browning rate of the callus during callus induction can be effectively reduced, and the tissue culture seedling raising efficiency is improved.

The invention provides application of a bacteriostatic agent in open type tissue culture and rapid propagation of polyploidy bamboo reeds, and relates to the technical field of tissue culture and rapid propagation of plants, the bacteriostatic agent is applied to the open type tissue culture and rapid propagation process of the polyploidy bamboo reeds, and the use method is that the bacteriostatic agent is added into a culture medium for use or sprayed or oscillated and flushed for use, and the bacteriostatic agent is applied to the open type tissue culture and rapid propagation process of the polyploidy bamboo reeds. The bacteriostatic agent is formed by mixing isothiazolinone and sodium dehydroacetate, the mass addition ratio of the isothiazolinone to the sodium dehydroacetate is (8-5): (2-5), and the method comprises the following steps: S1, primary culture of explants; s2, multiplication culture of seedlings; s3, rooting culture, wherein each liter of the culture medium contains 0.05-1g of the bacteriostatic agent in the above steps. The method has the beneficial effects that under the condition that the method does not need to be controlled in a sterile environment and does not depend on the quality of an inoculator, a large amount of propagation of the polyploidy bamboo reed tissue culture seedlings is realized by adopting an open tissue culture technical mode, the productivity speed of a tissue culture center is improved, and the construction threshold and cost of the tissue culture center are reduced.

The invention provides a tissue culture method and an acclimatization and transplantation method of coptis chinensis, and belongs to the technical field of plant tissue culture. The tissue culture method of the coptis chinensis comprises the following steps: (1) taking a tender shoot of a coptis chinensis rhizome as an explant, sterilizing, and inoculating into a basic culture medium for culturing, so as to obtain a coptis chinensis sterile seedling; (2) inoculating the aseptic seedlings of coptis chinensis into an adventitious bud differentiation culture medium for culture to obtain adventitious buds of coptis chinensis; (3) inoculating the coptis chinensis adventitious buds into a proliferation culture medium for culturing to obtain coptis chinensis proliferation seedlings; and (4) inoculating the coptis chinensis multiplication seedlings into a rooting culture medium for culture to obtain coptis chinensis test-tube seedlings. The tissue culture and acclimatization and transplantation method provided by the invention can improve the propagation efficiency of the coptis chinensis, remarkably shorten the growth cycle of the coptis chinensis, provide technical support for rapid propagation and sustainable utilization of resources of the coptis chinensis, and have wide application prospects.

The invention discloses a dendrobium officinale unmanned inoculation tissue culture method and application thereof. Belongs to the technical field of tissue culture. The vertical gradient culture medium is adopted for the first time, and automatic conversion of the development stage is achieved; a culture medium osmotic pressure gradient design guides directional growth of a root system; according to the nutrition phase change technology, the agar concentration difference of an induction layer, a differentiation layer and a strong seedling layer is utilized, the physical characteristic gradient of a culture medium is achieved, unmanned inoculation tissue culture of dendrobium officinale seeds is achieved through automatic equipment and an optimized tissue culture process, and the problems that in the prior art, artificial inoculation is low in efficiency, high in pollution rate and cost and long in culture period are solved; the efficiency, the quality and the economic benefit of dendrobium officinale tissue culture are improved, and the requirements of large-scale factory production can be met.

The invention belongs to the technical field of plant biology, and particularly relates to a genetic transformation method based on oat immature embryos, which comprises the following steps: preparing an infection solution containing a target gene; taking immature caryopses of the oats 21-23 days after pollination; infecting the immature embryos with infection liquid, and co-culturing the immature embryos after infection is completed; after co-culture is completed, immature embryos are sequentially transferred into an L3-T culture medium and an L3-B5 culture medium to induce formation of calluses, and after the calluses are formed, the calluses are sequentially induced to be differentiated, rooted and hardened. According to the method, efficient genetic transformation of exogenous genes is realized by selecting proper oat immature embryos, proper culture conditions and proper transformation conditions. By means of the genetic transformation method, the genetic transformation efficiency of the oats is improved to 25% from 2%-3%. And meanwhile, the immature embryo is used as a transformation acceptor material, and the complete plant can be efficiently and stably regenerated by the method disclosed by the invention.

The invention relates to a Tulasnella sp. QYM-1, a culture method of dendrobium huoshanense and application of the Tulasnella sp. QYM-1, and belongs to the technical field of microorganisms. The invention provides the mucilaginoidea fungus QYM-1, and the mucilaginoidea fungus QYM-1 is separated from protocorm formed by symbiotic germination, can promote germination of dendrobium huoshanense seeds to form seedlings, has relatively strong specificity on culture of the dendrobium huoshanense seeds, can replace tissue culture to become a main technology for seedling culture of dendrobium huoshanense, and has a broad application prospect in the field of seedling culture of dendrobium huoshanense. And the method has extremely high application value in production. In addition, the mucilaginoidea fungus QYM-1 is low in production cost and suitable for large-area popularization and application, and has huge research potential in the aspects of regression of rare and endangered orchid plants, wild-imitating cultivation of medicinal orchid plants and the like.

The invention relates to the technical field of plant breeding, and particularly discloses a metabonomics-based alfalfa stress resistance gene analysis function and a breeding method. The core of the method is that screening is carried out by combining a tissue culture technology with metabolic intervention; an alfalfa explant is inoculated into an induction culture medium containing a specific metabolic pathway regulator for metabolic intervention culture; then transferring the obtained callus to a verification culture medium containing the metabolism regulator and an abiotic adversity stress factor at the same time, and carrying out metabolism mark verification and screening; and finally regenerating the screened cell line into a complete plant. According to the method, metabolic pressure is actively applied to the cellular level, and dual verification screening is implemented, so that early-stage, efficient and accurate breeding of the stress-resistant alfalfa material is realized, and the technical problems of long period, low efficiency and insufficient utilization of metabolic information in traditional breeding are solved.

The present invention relates to the fields of tissue culture and microbial technology, and particularly to a seedling raising method for inoculating disease-preventing and growth-promoting bacteria to improve the disease resistance of crops, comprising the following steps: Step 1, culturing tissue culture seedlings to be inoculated with disease-preventing and growth-promoting bacteria by means of tissue culture; Step 2, inoculating disease-preventing and growth-promoting bacteria at the roots of the tissue culture seedlings; Step 3, randomly inspecting to determine whether the disease-preventing and growth-promoting bacteria have successfully colonized in the tissue culture seedlings. If the colonization is successful, proceed to Step 5. If the colonization is unsuccessful, first proceed to Step 4 and then to Step 5; Step 4, synchronously inoculating auxiliary bacteria when inoculating the disease-preventing and growth-promoting bacteria in Step 2; Step 5, domesticating and transplanting the tissue culture seedlings in which the disease-preventing and growth-promoting bacteria have successfully colonized; The present invention can colonize the disease-preventing and growth-promoting bacteria in the tissue culture seedlings in advance, reduce the entry of late pathogens into the crop body, and achieve the purpose of disease-resistant seedling raising.

The invention relates to a germination method of cibotium barometz spores and a breeding method of cibotium barometz, and belongs to the technical field of plant culture. The breeding method comprises the following steps: disinfection: disinfecting cibotium barometz spores for 5-10 minutes by using a 10% sodium hypochlorite solution; spore germination, wherein a used germination culture medium comprises a 1 / 4 MS culture medium, 4.5-7 g.L <-1 > agar and 0-30 g.L <-1 > sucrose; sporophyte differentiation: a used sporophyte differentiation culture medium comprises a 1 / 2 MS culture medium, 0 to 1 mg.L <-16 >-BA, 0.5 to 1 mg.L <-12 >, 4-D, 4.5 to 7 g.L <-1 > of agar and 25 to 30 g.L <-1 > of sucrose; sporophyte seedling strengthening: a used sporophyte seedling strengthening culture medium comprises a 1 / 4 MS culture medium, 4.5 g.L <-1 > to 7 g.L <-1 > agar, 30 g.L <-1 > sucrose and 1 g.L <-1 > to 2 g.L <-1 > activated carbon; acclimatization and transplantation: sealing the tissue culture seedlings, gradually releasing the sealing, and then transferring the tissue culture seedlings into a specific mixed matrix for culture to obtain commercial seedlings. According to the method, the spore germination rate can be increased by adopting a specific disinfection procedure, and the effects of more seedlings and good seedlings can be achieved by adopting a specific culture medium and seedling hardening configuration in each stage.

The invention provides a cultivation method of ornamental small ginger, and relates to the field of plant cultivation, and the method comprises the following steps: tissue culture seedling cultivation: adopting a culture medium comprising a basic culture medium, a plant growth regulator, an organic additive, an antioxidant and trace elements for cultivation to obtain tissue culture seedlings. Performing potting cultivation: transferring the tissue culture seedlings into a potting container on which a mixed substrate is uniformly laid, and performing cultivation, wherein the mixed substrate comprises charcoal particles, modified zeolite particles, a mycorrhizal fungus inoculant, gelatin slow-release particles, fermented soybean meal and graphene modified cellulose; in the cultivation process, irrigation, fertilization and environment regulation and control are carried out regularly. According to the method, high-quality tissue culture seedlings are obtained by using a culture medium with specific components, and growth balance of ginger plants is adjusted and overgrowth of the plants is inhibited by adjusting a planting matrix and regulating and controlling a specific culture method, so that small potted ginger plants for ornamental use are obtained.

The invention discloses an artemisia apiacea tissue culture and one-step seedling forming method, which comprises the following steps: cutting stems with axillary buds of artemisia apiacea as explants, and carrying out sterilization and inoculation pretreatment; inoculating the explants on an induction culture medium for culturing, and inducing the explants to generate cluster buds; transferring the germinated cluster buds to a proliferation culture medium for proliferation culture of the cluster buds; and cutting the proliferated non-rooted seedlings, and transferring to a rooting culture medium for rooting culture. According to the method, single high-concentration excitation 6-BA is used as a hormone for inducing the culture medium, axillary bud stem explants can be directly induced to generate cluster buds, and the generation of calluses is remarkably reduced. Meanwhile, in the subculture multiplication culture, after the subculture stage and the concentration of the used 6-BA are flexibly adjusted, the generation of calluses and the generation of vitrification phenomena can be further reduced. According to the method, the cluster buds can be rapidly rooted, so that a large number of seedlings are obtained, and the method has the remarkable advantages of saving time and manpower and material resource cost in production.

The invention discloses a podophyllum hexandrum callus rapid proliferation method, and belongs to the technical field of plant tissue culture, the podophyllum hexandrum seed dormancy is broken through a sand storage method, the seeds are soaked in distilled water and a gibberellin solution and then are fully and uniformly mixed with river sand, after storage and germination acceleration, the seeds are sown in soil added with an equal amount of matrix, and podophyllum hexandrum seedlings are obtained through culture; fresh leaves of podophyllum hexandrum seedlings are taken as explants, the explants are disinfected and cut into small pieces, the small pieces are cultured on an MS culture medium added with hormone for 30 d to induce callus, the induced callus is cultured and proliferated at the optimal temperature of 15 DEG C, and the callus is inoculated to the same fresh culture medium after being cultured for 20 d to prevent browning, so that the high-quality callus is obtained. And then replacing a fresh culture medium every 20 days to realize rapid and mass proliferation of the podophyllum hexandrum callus. Through the method, a large amount of high-quality podophyllum hexandrum callus can be rapidly obtained, and sufficient materials are provided for podophyllotoxin extraction and medicinal value development.