126results about "Plant cells" patented technology

The present application relates to the field of plant tissue culture technology, and particularly discloses a lily stem cell abundance ex vivo regulation method. The lily bulb is used as the starting material, stem cell magnitude amplification and regulation culture can produce lily tissue organ materials with high stem cell abundance. The present application provides sterile lily materials with high stem cell abundance, which has the characteristics of strong regenerative ability, high proliferation rate, etc., is the basis for protoplast preparation and high-efficiency regeneration, and is suitable for different lily varieties, and provides technical support for cell engineering breeding, seedling scale breeding, etc.

The disclosure relates to novel soybean plants, plant parts, and nucleotide sequences in soybean plants comprising an amorphic allele of a RPF1 gene, along with methods of using and making the same.

This invention discloses a method for inducing and culturing burdock stem cells and stem cells, belonging to the field of plant biotechnology. Using burdock roots as explants, the cambium is determined by staining, and the cambium is obtained using enzymatic digestion. The cambium is then inoculated into a specific ratio of induction solid medium and cultured in the dark to efficiently induce primary stem cells. These primary stem cells are then transferred to a proliferation solid medium for subculture and selection to obtain and maintain a vigorous, soft stem cell line. The solid stem cell line is further transferred to a liquid medium for shaking culture to establish a suspension stem cell line. This invention provides high induction efficiency, stable stem cell characteristics, and good reproducibility, offering a high-quality starting material and technical platform for in vitro preservation of burdock germplasm, large-scale production of secondary metabolites, and genetic transformation.

This invention discloses a stable and efficient method for preparing protoplasts from *Pseudolarix amabilis*. The method directly uses etiolated *Pseudolarix amabilis* seedlings as material to prepare protoplasts, requiring no other aseptic procedures, and the entire process is simple and convenient. Furthermore, by setting different buffer component ratios and a specific mannitol concentration, this invention achieves stable maintenance of the osmotic pressure of *Pseudolarix amabilis* seedling cells during the separation process. Experimental verification shows that the protoplast yield using this method is 1.32 × 10⁻⁶. 5 The protoplasts were prepared at a density of 1 protoplast per ml, with 95% of them intact and undamaged, resulting in significantly improved quality and activity. Furthermore, the enzymatic hydrolysate described in this invention overcomes the difficulty of enzymatic hydrolysis caused by the high degree of leatheriness in *Pseudolarix amabilis* leaf tissue. This method is suitable for widespread application in the field of herbaceous plant biotechnology.

The invention relates to a composition for making a tea beverage, the composition comprising tea which may be ground tea in combination with plant cell culture grown in vitro in a large scale. The plant cell culture grown in vitro in a large scale may be derived from any plant, such as a fruit including but not limited to grape berry, pomegranate, olive, and the like. The composition may be inserted into a pod and be used in a machine or inserted into a tea bag or a container.

The present application relates to a method for transforming corn, comprising: the primary callus obtained by the method for inducing corn callus, using the method for obtaining corn suspension cell line to obtain corn suspension cell line; agrobacterium infection corn suspension cell group in the corn suspension cell line. The method for transforming corn of the present application first induces the primary callus from corn proembryo, quickly obtains the suspension cell line, shortens the experimental period to 40-50 days, is simple and convenient to operate, has good repeatability, simultaneously makes the corn genetic transformation not affected by the season, meets the needs of annual stable transformation; then uses agrobacterium infection to transform the corn suspension cell line, and regenerates seedlings again; the transformation efficiency can reach about 30%, the transgenic positive rate can reach about 90%, and the single copy rate is about 50%; since the pressure on corn explant (proembryo) supply is significantly reduced, the human and material resources consumption required for culturing the explant is greatly reduced, and large-scale genetic transformation is realized.

The present invention provides at least one stevia plant with genetic characteristics (1) to (5).

The present invention relates to a single-phase, spherical edible microcarrier for the three-dimensional culture of stem cells intended for the preparation of cultured meat. The microcarrier comprises a homogeneous mixture of edible ionic polysaccharide (0.375-1.5%), edible protein (1-2.5%), edible polymer (0.025-0.1%) and water (95.9-98.6%). The invention also relates to a method for preparing these microcarriers by mixing three solutions: ionic polysaccharide in saline solution, edible protein in saline solution and edible polymer in water. The mixture is subjected to a process involving microspraying or electrospraying with calcium chloride in order to form spherical microcarriers of 50-300 micrometres. The design of the single-phase, spherical edible microcarrier simplifies the production of cultured meat by dispensing with the need for core-shell structures, thereby improving cell anchorage efficiency and facilitating cell recovery.

The application belongs to the technical field of cell biology, and particularly relates to a solution rich in Sargassum fusiforme nano-vesicles and a preparation method and application thereof. The solution rich in Sargassum fusiforme nano-vesicles is prepared by a differential centrifugation combined with hollow fiber membrane concentration, and the obtained solution rich in Sargassum fusiforme nano-vesicles can play an anti-inflammatory role, inhibit high-fat diet induced fatty liver and osteoporosis, and can also be used for preparing a mask with soothing, moisturizing and anti-wrinkle effects.

Compositions and methods are provided for influencing expression of endogenous genes or alleles in plants only in a heteroallelic state, such as in hybrid crops, while retaining unaffected expression of the endogenous genes or alleles in a homozygous state, by editing untranslated regions of the endogenous genes through genomic editing techniques.

It provides a platform for regulating the translation of any protein of interest within eukaryotic cells. [Solution] This disclosure provides a gene construct comprising a recombinant internal ribosome entry site (IRES) that can be used as a riboswitch to functionally regulate the translation of an mRNA sequence encoding a protein of interest. In other aspects, this disclosure provides recombinant cells, methods, kits and systems that utilize it to provide a platform for regulating the expression of essentially any protein of interest in eukaryotic cells, for example.

The present invention provides an apparatus, method, and program for extracting multiple images of the same microorganism from multiple captured images. [Solution] In the system 10, the discrimination device 60 includes an acquisition unit that receives multiple images of the flow channel 24 through which microorganisms in the flow channel device 20 flow, and an extraction unit that extracts multiple images of the same individual microorganism from the multiple images. The flow channel through which microorganisms flow may have a vortex generator that generates vortices in the imaging range 26 that captures multiple images within the flow channel. The acquisition unit receives multiple images of the flow channel through which microorganisms flow, taken at different timings. The acquisition unit also receives multiple images taken from two or more different directions.

The present application relates to the use of extracellular vesicles derived from Artemisia annua in skin care products, in particular the use includes any one of the following: (1) the use in the preparation of a product having skin barrier damage repair function; (2) the use in the preparation of a product having moisturizing function. The present application finds that the extracellular vesicles derived from Artemisia annua have excellent function of improving skin barrier damage repair and have excellent moisturizing effect.

This invention discloses a method for preparing and applying engineered *Anoectochilus roxburghii* exosomes for targeted therapy of breast cancer, relating to the biomedical field. The outer surface of the *Anoectochilus roxburghii* exosomes is modified with dual-targeting peptides, and the membrane is loaded with chemotherapeutic drugs. The engineered *Anoectochilus roxburghii* exosomes can target and deliver functional nucleic acid molecules and internalized chemotherapeutic drugs, further enhancing the regulation of related pathways to inhibit breast cancer cell proliferation, migration, and apoptosis for tumor treatment. The engineered *Anoectochilus roxburghii* exosomes of this invention have advantages such as simple preparation method, precise targeting of tumor cells and organelles, significant anti-tumor growth efficacy, high specificity, good biocompatibility, and high delivery efficiency, and can be used as a novel nanomedicine for tumor therapy.

The present application belongs to the field of plant biotechnology, and particularly relates to a Korean pine cell fusion method based on chemical induction combined with a magnetic bead method. The method comprises the following steps: S1, preparing first protoplasts and second protoplasts; S2, preparing passivated first protoplasts and passivated second protoplasts; S3, mixing the passivated first protoplasts with the passivated second protoplasts in equal volumes after the passivated first protoplasts are cultured with a sodium silicate aqueous solution to obtain mixed protoplasts; S4, performing electrofusion on the mixed protoplasts in a cell fusion instrument to obtain fusion products; and S5, screening hybrid cells from the fusion products by using a magnetic bead method. The present application adopts a double screening mechanism of chemical passivation and magnetic bead screening, inactivates parent cells by treating with iodoacetamide and rose Bengal B, and then specifically enriches hybrid cells by using the magnetic bead method, so that the acquisition rate and screening accuracy of hybrid cells are significantly improved.

The application provides a culture medium for producing dendrobium stem cell exovesicles and an application thereof, relates to the technical field of biology, and the culture medium for producing dendrobium stem cell exovesicles comprises 0.1-0.5 mg / L naphthalene acetic acid, 0.05-0.2 mg / L thidiazuron, 0.5-5 muM sodium selenate and 1-10 mg / L glutathione. The culture medium for producing dendrobium stem cell exovesicles and the method for preparing dendrobium stem cell exovesicles by using the culture medium for producing dendrobium stem cell exovesicles provided by the application have made significant progress in the yield and quality of dendrobium stem cell exovesicles. Meanwhile, the prepared dendrobium stem cell exovesicles have significant DPPH free radical scavenging capacity and improve the healing capacity of skin fibroblasts.

This disclosure provides systems and methods for transposing a cargo nucleotide sequence into a target nucleic acid site in a target nucleic acid. These systems and methods may include a double-stranded nucleic acid comprising a cargo nucleotide sequence, wherein the cargo nucleotide sequence is configured to interact with a recombinase complex; an effector complex comprising at least one manipulated guide polynucleotide configured to hybridize to an effector and target nucleic acid; and a recombinase complex configured to recruit the cargo nucleotide to the target nucleic acid site.

This invention belongs to the fields of bone disease treatment and natural medicine, and provides the application of Andrographis paniculata-derived nanovesicles in the preparation of drugs for treating postmenopausal osteoporosis. The Andrographis paniculata-derived nanovesicles are extracted from Andrographis paniculata by differential centrifugation and PEG precipitation, with a particle size of 50-200 nm, and contain protein, RNA, and lipid components. The Andrographis paniculata-derived nanovesicles can significantly improve the bone microstructure of ovariectomized mice, increase bone volume fraction and trabecular bone number, reduce trabecular bone separation, inhibit osteoclast formation, and promote bone defect repair, with good in vivo safety. The Andrographis paniculata-derived nanovesicles contain extremely low levels of andrographolide, but their activity in promoting osteogenic differentiation and inhibiting osteoclast differentiation is significantly better than that of andrographolide monomer, indicating that their efficacy comes from the entire vesicle rather than a single component. This invention provides a safe and effective new drug regimen for the treatment of postmenopausal osteoporosis.

The application belongs to the technical field of biological medicine, and particularly relates to a curcuma-mesenchymal stem cell fusion exosome and application thereof in treating diabetic wounds. The curcuma exosome and the mesenchymal stem cell exosome are fused by a short-time and low-power ultrasonic fusion technology of an ultrasonic instrument on a mixed exosome suspension, and a fusion exosome is prepared. The fusion exosome is further prepared into a fusion exosome gel, so that the problem of low delivery efficiency of the exosome and the mesenchymal stem cell exosome when used alone is improved, and the healing effect of the diabetic wound is significantly improved.

The application discloses a kind of protein nutrition food and the application method of by-product thereof. The application provides a kind of general pretreatment method suitable for multiple protein sources: protein source raw materials are processed into suitable form after being purified, deodorized, blanched and shaped;The deodorization treatment adopts one or more combinations of physical, chemical or biological methods. Based on this method, the application develops a series of protein nutrition tofu, cheese, yogurt, milk powder and wine products, the protein source covers animal source, plant source and microbial source, and realizes the resource utilization of yellow sludge water and whey by-products, and expands its application in the field of biotechnology such as microbial culture medium and plant tissue culture. The pretreated protein source can also be used as a semi-finished product raw material for pre-prepared dishes, 3D printing food ink and other emerging fields. The application provides a general preparation method for the food application of various non-traditional protein sources.

This provides useful regulatory elements in the fields of plant molecular biology and plant genetic engineering. [Solution] The present invention provides recombinant DNA molecules and constructs useful for regulating gene expression in plants, as well as their nucleotide sequences. The present invention also provides transgenic plants, plant cells, plant parts, and seeds containing recombinant DNA molecules operably linked to heterologous transcriptionable DNA molecules, and also provides methods for using them.

The application provides application of a carrot-derived extracellular vesicle-like particle in preparation of a skin wound repair product, and belongs to the technical field of biological medicine. The extracellular vesicle-like particle has good water dispersibility and low-temperature storage stability; can be taken by dermal fibroblasts, vascular endothelial cells and the like, can reduce cell apoptosis, improve cell activity, promote proliferation, migration and blood vessel formation related functions under high-sugar high-fat and the like damage conditions, thereby synergistically improving the functions of dermal fibroblasts and vascular endothelial cells, and has certain antibacterial capacity, thereby being beneficial to skin wound healing, and providing a scheme and a clinical conversion idea for wound treatment.

This invention discloses the application of Lycoris radiata exosomes in the preparation of drugs for treating heterotopic ossification, belonging to the field of biomedical technology. The Lycoris radiata dried exosomes (La-dEXOs) prepared by this invention, verified through osteoclast and osteoblast differentiation experiments and animal experiments, show that the La-dEXOs prepared by this invention can improve heterotopic ossification in rats by promoting osteoclast differentiation and inhibiting osteoblast differentiation. Experimental results indicate that La-dEXOs have the potential to be developed into related pharmaceutical products for the prevention and treatment of heterotopic ossification.

The application provides a loess soup self-assembled nanocapsule which is obtained by using a differential centrifugation method on a water decoction of loess soup; the particle size is 100-200nm, and the Zeta potential is (-10)-(-40)mV. The application further provides a preparation method and application of the loess soup self-assembled nanocapsule. The loess soup self-assembled nanocapsule has a significant anti-inflammatory effect, is better in targeting, is controllable in drug distribution, is higher in bioavailability, is more explicit in effective components, and is controllable in quality.

The application belongs to the technical field of plant cultivation and particularly relates to a Blumea riparia growth medium, a tissue culture medium, a tissue culture method and application. The Blumea riparia growth medium takes MS medium as a basic medium and further comprises 0.2-0.3 mg / L NAA, 0.08-0.16 mg / L DA-6, 10 mg / L vitamin C, 20 mg / L myo-inositol, 30 g / L sucrose and 6 g / L agar. DA-6 and NAA are jointly applied to improve the rooting efficiency, root length and plant height of Blumea riparia. The Blumea riparia germplasm with high content of levorotatory borneol is used as an explant to carry out tissue culture and seedling raising, the Blumea riparia tissue culture seedling has high content of levorotatory borneol, and Blumea riparia stable genetic good seedlings can be produced.

A standardized plant extract is produced by cultivating undifferentiated or differentiated in vitro cultures of Haberlea rhodopensis Friv. (HR), followed by harvesting the biomass and extracting bioactive compounds and primary and secondary metabolites, including organic acids, fatty acids, amino acids, sterols, free phenols, sugars, and polyphenols. The extraction process is performed under controlled conditions to yield an extract with a predominant myconoside content of 70% to 96% in the polyphenolic fraction, constituting 18% to 35% of the total extract. The extract may be combined with glycerol to form a composition with standardized myconoside content. The resulting extract or biomass is then incorporated into products for the pharmaceutical, cosmetic, or food industries. This method provides a consistent, renewable, and sustainable source of raw material, eliminating dependence on wild plant populations and seasonality, and delivers valuable health-protective properties.