1441 results about "Methionine biosynthesis" patented technology

A method of thermally processing a tobacco material is provided, the method including the steps of (i) mixing a tobacco material, water, and an additive selected from the group consisting of lysine, glycine, histidine, alanine, methionine, glutamic acid, aspartic acid, proline, phenylalanine, valine, arginine, di- and trivalent cations, asparaginase, saccharides, phenolic compounds, reducing agents, compounds having a free thiol group, oxidizing agents, oxidation catalysts, plant extracts, and combinations thereof, to form a moist tobacco mixture; (ii) heating the moist tobacco mixture at a temperature of at least about 60° C. to form a heat-treated tobacco mixture; and (iii) incorporating the heat-treated tobacco mixture into a tobacco product. Heat-treated tobacco composition prepared according to the method are also provided, such as heat-treated smokeless tobacco composition comprising a tobacco material, water, flavorant, binder, and filler, the heat-treated smokeless tobacco composition having an acrylamide content of less than about 2000 ppb.

The invention relates to an enzymology measuring method and reagent with circular increment technology, applied to measure the content of homeotypic cysteine in the measured sample. The character is: with a circular increment technology, increases the measurement sensitivity, thus it can carries on automatic measurement to the homeotypic cysteine in the sample liquid like normal enzymology diagnosing agent. The homeotypic cysteine in the sample liquid reacts with the S-adenosine-L-methionine repeatedly and circularly under the effect of homeotypic cysteine methyl kinase and adenosine homeotypic cysteine, generates the adenosine, the speed of the adenosine generation is in direct proportion to the homeotypic cysteine in the sample, through the generation speed, the aim to measure the content of the homeotypic cysteine in sample can be achieved.

The present invention relates to a highly concentrated, stable pharmaceutical formulation of a pharmaceutically active anti-HER2 antibody, such as e.g. Trastuzumab (HERCEPTIN™), Pertuzumab or T-DM1, or a mixture of such antibody molecules for subcutaneous injection. In particular, the present invention relates to formulations comprising, in addition to a suitable amount of the anti-HER2 antibody, an effective amount of at least one hyaluronidase enzyme as a combined formulation or for use in form of a co-formulation. The formulations comprise additionally at least one buffering agent, such as e.g. a histidine buffer, a stabilizer or a mixture of two or more stabilizers (e.g. a saccharide, such as e.g. α,α-trehalose dihydrate or sucrose, and optionally methionine as a second stabilizer), a nonionic surfactant and an effective amount of at least one hyaluronidase enzyme. Methods for preparing such formulations and their uses thereof are also provided.

These and other objects of this invention are achieved by providing a novel method and compositions for the clinical treatment of chronic inflammatory diseases. This invention involves use of systemically administered compositions which include primary amine derivatives of benzoic acid as carbonyl trapping agents. These primary therapeutic agents act by chemically binding to and sequestering the aldehyde and/or ketone products of lipid peroxidation. Increased levels of lipid peroxidation have been repeatedly demonstrated as a part of the non-enzymatic "inflammatory cascade" process which underlies the secondary etiology of chronic inflammatory diseases. p-Aminobenzoic acid (or PABA) is an example of the primary therapeutic agent of the present invention. PABA has a small molecular weight, is water soluble, has a primary amine group that reacts with carbonyl-containing metabolites under physiological conditions and is tolerated by the body in relatively high dosages and for extended periods. The carbonyl sequestering agents are used in combination with at least one co-agent so as to produce an additional beneficial physiological effect of an anti-inflammatory nature. Such compositions are administered systemically entirely via the oral route. Co-agents of the present invention include anti-oxidants and free radical trapping compounds (e.g., alpha-tocopherol), compounds having indirect anti-oxidant activity (e.g., selenium), vitamins (e.g., pyridoxine HCl), compounds which facilitate kidney drug elimination (e.g., glycine), metabolites at risk of depletion (e.g., pantothenic acid), sulfhydryl containing chemicals (e.g., methionine), compounds which facilitate glutathione activity (e.g., N-acetylcysteine), and non-absorbable polyamine co-agents (e.g., chitosan).

The five component composition consisting essentially of: (1) Water soluble antioxidant vitamin C or ascorbic acid, or any of its forms or derivatives, or mixtures thereof. (2) Oil soluble antioxidant vitamin E or Alpha-tocophorol, or any of its forms or derivatives, or mixtures thereof. (3) The element selenium, or a chemical (or composition) containing it, or mixtures thereof. The most preferred chemical containing selenium is dimethyl selenide and mixtures thereof. The words "dimethyl selenide" here and hereinafter mean dimethyl selenide and/or it's oxidation products, including dimethyl selenoxide. (4) A sulfur amino acid, in any form, or a sulfur peptide, or a sulfur protein, or any of their derivatives, or mixtures thereof. The mixture of methionine and cysteine, which contains as impurities some seleno-methionine and some selenocysteine, is preferred,-the tripeptide glutathione containing cysteine is also preferred. (5) Another antioxidant, other than vitamin C and other than vitamin E, which is synthetic or natural and water soluble or oil soluble, or a mixture of such antioxidants, or a combination of such forms thereof. The mixtures of butylated hydroxyanisole and ethoxyquin is preferred.

The problem to be solved is to provide an antibody-containing formulation which is stable and suited for subcutaneous administration, wherein dimerization and deamidation is prevented during long-term storage. The present application is directed to a stable antibody-containing liquid formulation characterized by containing arginine and methionine.

A microorganism strain suitable for fermentative production of L-methionine and preparable from a starting strain, which comprises increased activity of a yjeH gene product or of a gene product of a yjeH homolog, compared to the starting strain.

Personal care composition comprising at least one hair growth regulating compound selected from the group consisting of glyceryl dilaurate, apigenin, tetrahydrocurcumin, oleanolic acid, azelaic acid, sulforaphane, canavanine, pyridoxal 5-phosphate, phytic acid, tannic acid, grape seed extract, NG-nitro-L-arginine-methyl ester, benzamidine, sodium butyrate, betulinic acid, polyornithine, polyarginine, fisetin, jasmonates, methyl-jasmonate, cis-jasmone, caffeic acid phenethyl ester, delphinidin, ethyl abietate, esculetin, sorbic acid methyl ester, canaline, N-formyl-methionine, N-formyl-alanine, taurine, palmitoyl carnitine, undecanol, undecylenic acid, rutin, fusidic acid, phenyl pyruvic acid, L-isoleucine, phenyl glycine, silibinin, silymarin, L-ascorbic acid-6-palmitate, N-undecylenoyl-L-phenylalanine, and salts, derivatives and mixtures of any of the foregoing; and a dermatologically-acceptable carrier.

The invention relates to a layer foodstuff used for producing health care eggs, consisting of the following components in weight portion: 120 to 160 portions of calcium hydrogen phosphate, 250 to 350 portions of mountain flour, 50 to 60 portions of common salt, 20 to 40 portions of saleratus, 15 to 20 portions of choline, 35 to 40 portions of complex trace elements, 8 to 10 portions of complex vitamins, 10 to 15 portions of lysine, 25 to 30 portions of methionine, 220 to 400 portions of pine needle meal, 1 to 3 portions of bacillus subtilis, 8 to 10 portions of copper sulfate, 2 to 5 portions of phytase, 1 to 5 portions of yeast selenium, 3 to 6 portions of vitamin E, and 250 to 400 portions of Chinese traditional herbal medicine preparation. The invention adds nutritive additives etc. into the foodstuff; therefore the egg laid by the layer contains such beneficial elements as selenium, vitamin E, and is rich in Omega-3 polyunsaturated fatty acid and low in cholesterol.

Polymerase chain reaction primers and methods directed to detecting the EGFR mutant C→T at the position corresponding to base 2369 of EGFR cDNA. The invention provides a PCR primer that hybridizes under suitable PCR conditions to a polynucleotide sequence 5′ in each respective strand to a mutation of an EGFR gene that encodes a substitution of threonine by methionine at position 790 of the EGFR polypeptide. The invention also provides a PCR primer hybridizes to a sequence that includes a mutant T at the position corresponding to base 2369 of EGFR cDNA but not to a second EGFR polynucleotide containing a wild type C. The invention provides several methods and kits for detecting a mutant epidermal growth factor receptor (EGFR) gene in a sample comprising probing the sample with a means for selectively detecting a nucleotide sequence comprising a mutant T at the position corresponding to base 2369 of EGFR cDNA, and identifying that the base at said position is T. These methods and kits are also useful to predict resistance to the therapeutic effects of gefitinib or erlotinib in a subject suffering from or suspected of having a cancer.

Provided are compositions and methods for increasing diet induced thermogenesis. Typically, the compositions are comprised of L-histidine, L-isoleucine, L-leucine, L-lysine, L-methionine, L-phenylalanine, L-valine and L-threonine.

The invention relates to polynucleotides comprising polynucleotide sequences corresponding to the metD gene and parts thereof that encode polypeptide sequences and parts thereof possessing varying degrees of MetD transcription regulator activity, methods for preparation of L-amino acids, and methods of screening and amplifying polynucleotides encoding polypeptide sequences which comprise varying degrees of MetD transcription regulator activity. Further, the invention relates to animal food additives based on fermentation liquor and containing L-methionine, and to the preparation of such additive.

Methods are provided for detecting methionine aminopeptidase (MAP) activity and for detecting inhibitors of MAP. The methods utilize a peptide comprising an N-terminal methionine which can be cleaved from the peptide by MAP, and a C-terminal detection moiety which is released by a second peptidase only if the N-terminal methionine has been cleaved from the peptide. When the peptide is combined with MAP and the second peptidase, the detection moiety is released, while the addition of a MAP inhibitor will inhibit the release of the detection moiety. Reaction mixes, peptides, and kits which are useful for practicing the methods are also provided.

Compositions of S-adenosyl-L-methionine with additives are disclosed.

The invention discloses an organic selenium biologics and its preparation method, and method for producing selenium-rich vegetable using the same, wherein the preparation is prepared from water and the following components (by weight percentage) through mixing and fermentation, selenium dioxide : sodium hydrogen carbonate : methionine : brown sugar : carbamide : monobasic potassium phosphate : magnesium sulfate : manganese sulfate : calcium chloride = 35-45 : 35-45 : 10-20 : 45-55 : 1-10 : 1-10 : 1-4 : 1-4 : 1-10, the method of application comprises, diluting the organic selenium biologics to 50-200 times by charging water, spraying diluted organic selenium biologics liquid onto the vegetable leaves, stems or roots. The vegetable exerted by the organic selenium biologics contains rich selenium.

The subject invention relates to the cloning, expression and isolation of recombinant forms of beta-amyloid protein containing a N-terminal methionine (or one or more amino acids) as well as to methods of using this recombinant protein in the production of therapeutic antibodies, in the identification of therapeutic small molecules, and in the performance of diagnostic assays.

The present invention relates to the use of recombinant homoserine transsuccinylase enzymes with altered feedback sensitivity (MetA*) and possibly recombinant S-adenosyl methionine synthetase enzymes with reduced activity (MetK*) for the production of methionine, its precursors or derivatives thereof.

The invention discloses microbial feed additive used for pigs, and a preparation process thereof; the prescription is as follows: 1 portion of bacillus licheniformis, 1 portion of bacillus subtilis, 2 portions of bifidobacterium, 2 portions of enterococcus faecalis, 2 portions of enterococcus faecium, 1 portion of boas-oppler bacillus, 1 portion of lactobacillus casei, 1 portion of lactobacillus lactis, 1 portion of plant lactobacillus, 1 portion of candida utilis, 1 portion of saccharomyces cerevisiae, 80kg to 100kg of panela, 3kg to 5kg of salt, 5kg to 8kg of vinegar, 3kg to 5kg of lysine, 1kg to 2kg of methionine, and 1000L of pure water; the weight of each portion of strain is 2g to 5g. The preparation process is as follows: strain preparation, nutrient broth preparation, inoculated fermentation and check. The invention reduces the quantity of the used individual strain, and the strain quality is safe and reliable. The production process is simple and feasible, the effect is obvious; therefore, the invention has promotion value.

The invention discloses a laying duck feed and a preparation method thereof. The laying duck feed consists of the following components in part by weight: 50 to 65 parts of wheat, 5 to 15 parts of fine rice bran, 6 to 18 parts of soya bean meal, 2 to 8 parts of cottonseed crumb, 1 to 5 parts of rapeseed meal, 1 to 5 parts of corn distillers dried grains with soluble, 2 t o12 parts of mountain flour, 0.5 to 3 parts of calcium monohydrogen phosphate, 0.05 to 1 part of table salt, 0.1 to 1 part of 65 percent lysine, 0.05 to 1 part of methionine, 0.05 to 0.3 part of complex enzyme and 0.5 to 2 parts of premix. In the feed, corn is not used; the wheat is taken as a main raw material, and is compatible with plant protein, amino acid, fermentation product, calcium, and various minerals and vitamins in proper proportion; the laying rate of the laying duck and egg weight can be effectively improved; and the productivity of the laying duck is improved. Moreover, the laying duck feed can greatly reduce the feeding cost of the laying duck.

Compositions for generating a cooked flavor in a foodstuff, comprising specified flavor precursors that react on heating to generate the flavor and maintain a reactive association after inclusion in the foodstuff. The compositions may include combination of a sulphur source, e.g. hydrogen sulphide, methane thiol, a sulfur-containing amino acid, thiamine, cystine, sodium sulphide, ammonium sulphide, ammonium polysulphide, onions, garlic, shallots, eggs, methionine, and mixtures thereof, and at least one reductone, e.g. a furanone, a ketone, a pyrone, an aldehyde, a carbonyl compound, isomaltol, maltol, pyruvaldehyde, hydroxyacetone, 3-deoxyglucosone, 5-hydroxy-5,6-dihydromaltol, 2,3-butanedione, 3-hydroxy-2-butanone, a process flavor, cooked vegetable concentrates, soy sauce, and mixtures thereof.

The invention provides a method of synthesizing a diketopiperazine of the formula:

wherein:

• • R¹ is —CH₂COR³, or —CH₂CH₂COR³;
  • R² is the side chain of an amino acid selected from the group consisting of glycine, alanine, valine, leucine, isoleucine, serine, threonine, aspartic acid, asparagine, glutamic acid, glutamine, lysine, hydroxylysine, histidine, arginine, phenylalanine, tyrosine, tryptophan, thyroxine, cysteine, methionine, norvaline and ornithine;
  • R³ is —OH, —NH₂, —OR⁴, —NHR⁴, or —NR⁴R⁴; and
  • each R⁴ is independently an alkyl, aryl, alkylaryl, or arylalkyl.

The invention provides an aquatic feed which is prepared from soybean waste, steam heated fish meal, fish meal, brewer's yeast, squid meal, shrimp shell powder, choline chloride, flour, calcium dihydrogenphosphate, scallop powder, Ulva lacbuca Linnaeus powder, glutelin powder, phosphatidy icholine, fish oil, palmitic oil, maltose dextrin, amino acid fish grout, vitamins for shrimps, microelements, immunological polysaccharides, copper-methionine and complex enzyme. The invention also discloses the pre-maturing process for the aquatic feed.

The invention provides coating compositions that comprise antimicrobial agent comprising at least one hydroxy analog of methionine and a binder. The antimicrobial agents may be used as preservatives to inhibit a broad spectrum of microorganisms in the coating compositions.

The present invention is directed to novel compositions of bio-active compounds comprising 4-hydroxyisoleucine and one or more compounds selected from the group of amino acids, alkaloids, glycosides, volatile oils, saponins, sapogenins, mannans, flavonoids, fatty acids, vitamins and provitamins, minerals, and carbohydrates. Preferably, the novel compositions of bio-active compounds include 4-hydroxyisoleucine and one or more amino acids selected from the group consisting of arginine, aspartate, threonine, serine, glutamate, proline, glycine, alanine, cysteine, valine, methionine, isoleucine, leucine, tryptophan, phenylalanine, ornithine, proline, lysine, histidine, and gamma-aminobutyrate. The composition of bio-active compounds preferably include between about ten percent and about seventy percent of 4-hydroxyisoleucine and between about twenty percent and about forty percent of other amino acids. The bio-active compounds of the novel composition of the present invention may be derived, isolated, and/or extracted from Fenugreek seeds. A preferred method for extracting the bio-active compounds from Fenugreek seeds includes the steps of: (1) providing a plurality of Fenugreek seeds; (2) preparing the Fenugreek seeds; and (3) extracting a novel composition of bio-active compounds from the Fenugreek seeds, which include a preliminary extraction step and a secondary extraction step. The compositions of bio-active compounds have been found to be helpful in restoring healthy energy balance in humans and animals, aiding in weight management efforts, and for balancing blood sugar levels by way of assisting the body to make more efficient use of existing (i.e., endogenous) insulin.

The invention discloses a method for preparing methionine. The method for preparing the methionine concretely comprises the steps that cyanohydrins, carbon dioxide and ammonia or sodium cyanide, 3-(Methylthio)propionaldehyde, carbon dioxide and ammonia serve as raw materials to prepare hydantoin; alkaline hydrolysis is conducted on the hydantoin and then the hydantoin is acidized to pH 3.0-6.0 to obtain a compound of the methionine and salts; crystallization is conducted under the condition of temperature ranges from 8 DEG C to 45 DEG C, then the compound is separated to obtain a methionine crystal and salt mother liquor, the methionine crystal is washed by water and dried, and washing liquor is standby; finally, the salt mother liquor is concentrated under the condition of temperature which is lower than 60 DEG C, and then the salt mother liquor passes through a chromatographic column to obtain an inorganic salt and the methionine; the preparation method can efficiently separate the methionine and the salts, the recovery rate of the methionine can reach 99%, and the method is green and protects the environment, no methionine is resolved, no large number of acid and stink wastewater is discharged, and the purity of the inorganic salt is high and can be sold as a commodity.

In fabricated, thermally processed snack foods, the addition of one of a select group of amino acids to the recipe for the food inhibits the formation of acrylamide during the thermal processing. The amino acid can come from the group of cysteine, lysine, glycine, histidine, alanine, methionine, glutamic acid, aspartic acid, proline, phenylalanine, valine, and arginine and can be a commercially available amino acid or in a free form in an ingredient added to the food. Amino acids can be added to fabricated foods at the admix stage or by exposing raw food stock to a solution containing a concentration of the amino acid additive.

The invention relates to an antimold agent foodstuff, consisting of the following components in weight portion: 20 to 40 portions of Bacillus subtilis, 20 to 45 portions of astragalus polysaccharide, 200 to 350 portions of mycotoxins absobent, 20 to 50 portions of vitamin C, 20 to 50 Vitamin E, 5 to 15 portions of lysine, 5 to 15 portions of methionine, 200 to 250 portions of potassium chloride, and 180 to 520 portions of glucose. The invention adds nutritive additives and mycotoxins, etc. into the foodstuff, and can obviously intensify immunity of livestock and resist the attack of antibacterial enzyme to livestock.

A container comprising a closure means coated by an inert fluorinated material and containing a liquid pharmaceutical composition. In particular, the container comprises a closure means coated by TEFLON and contains a HSA-free Interferon-β formulation having the following composition: 30 to 100 μg/ml of interferon-β, an isotonicity agent, 0.1 to 2 mg/ml of Poloxamer 188, at least 0.12 mg/ml of L-Methionine and a buffer solution capable of maintaining the pH of the liquid formulation at a value between 3.0 and 4.0.

An Fc-binding polypeptide of improved alkali stability, comprising a mutant of an Fc-binding domain of Staphylococcus Protein A (SpA), as defined by SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO:3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:22, SEQ ID NO 51 or SEQ ID NO 52 wherein at least the asparagine or serine residue at the position corresponding to position 11 in SEQ ID NO:4-7 has been mutated to an amino acid selected from the group consisting of glutamic acid, lysine, tyrosine, threonine, phenylalanine, leucine, isoleucine, tryptophan, methionine, valine, alanine, histidine and arginine.

The invention discloses a pet dog food composition which comprises the raw materials based on parts by weight: 20-30 parts of corn, 10-20 parts of rice, 10-20 parts of soybean meal, 5-20 parts of chicken powder, 0.3-1 part of salt, 1-2 parts of chicken liver powder, 0.3-1 part of core material, 10-15 parts of milk powder, 1-10 parts of apple pulp and 5-15 parts of chicken grease; wherein the core material is formed by the raw materials based on parts by weight: 1 part of vitamin A, 0.1 part of vitamin D3, 2 parts of vitamin E, 2 parts of vitamin C fat, 0.1part of vitamin B1, 0.2 part of vitamin B2, 0.7 part of nicotinamide, 0.1 part of folic acid, 50 parts of choline, 10 parts of ferrum, 1 part of copper, 10 parts of zinc, 0.3 part of 1% iodine, 0.2 part of tea polyphenol, 100 parts of methionine, 215 parts of yeast powder, 500 parts of deep sea fish oil and 207.3 parts of carrier. The pet dog food has comprehensive and balanced nutrition as well as scientific and reasonable collocation, effectively improves the immunity of pet dogs, promotes growth and development of the dogs and protects the digestive tract to be healthy.