21 results about "Rutin" patented technology

This invention discloses a ternary composite foaming agent of flaxseed protein-fucose-rutin, its preparation method, and its application in meringue cookies. The foaming agent is a non-covalent complex formed by flaxseed protein isolate (FPI), fucoidan (FUC), and rutin (RU) through electrostatic interactions, hydrogen bonds, and hydrophobic interactions, with a mass ratio of FPI:FUC:RU = 1:2:(0.2~0.4). This invention forms a structurally stable composite system through the non-covalent interactions of FPI, FUC, and rutin, achieving a foaming height of 95.69±8.77%. The foam retention rate after 30 minutes is more than double that of natural FPI, and the interfacial tension is reduced to below 25 mN / m, overcoming the shortcomings of poor foaming properties and insufficient stability of single plant proteins. Even after high-temperature baking, the foam retention rate remains above 75%, meeting the processing requirements of meringue cookies.

The present application relates to the technical field of plant extract preparation, and particularly relates to a rutin extraction method. The rutin extraction method provided by the present application extracts rutin from sophora fruitling decoction pieces, and comprises the step of mixing the sophora fruitling decoction pieces with an extraction solvent for extraction. The sophora fruitling decoction pieces are prepared by the method comprising the following steps: after maturation, the sophora fruitling is allowed to rise, and then is subjected to cracking treatment to make the sophora fruitling petal crack; the fine powder passing through a 40-mesh sieve accounts for not more than 25% in mass fraction. Compared with the traditional sophora fruitling processing technology, the sophora fruitling decoction piece preparation method can effectively reduce the loss of rutin, and can significantly improve the leaching efficiency of rutin and reduce the leaching of impurities such as polysaccharides. The rutin extraction method provided by the present application can realize efficient extraction of rutin in sophora fruitling, reduce the dissolution of impurities during extraction, and realize green and efficient industrial production of sophora fruitling-derived rutin.

This invention provides a wheat aphid-resistant gene TaAHL19, its application, and a method for obtaining aphid-resistant wheat varieties. The nucleotide sequence of the wheat aphid-resistant gene TaAHL19 is shown in SEQ ID NO.1. The amino acid sequence encoded by the wheat aphid-resistant gene TaAHL19 is shown in SEQ ID NO.2. In this invention, overexpression of the TaAHL19 gene in wheat plants promotes the accumulation of rutin, an insect-resistant flavonoid, in wheat plants, thereby increasing the resistance of wheat plants to aphids without affecting wheat yield.

This invention discloses the use of cyanidin anthocyanins or their pharmaceutically acceptable salts, esters, and solvates in the preparation of drugs for the prevention and / or treatment of neurodegenerative diseases. The cyanidin anthocyanins are selected from cyanidin-3-O-rutin glycoside, cyanidin-3-O-sophoroside, or cyanidin-3-O-xyloside. These compounds can inhibit acetylcholinesterase activity, upregulate α7 nicotinic acetylcholine receptor expression, and activate the PI3K / AKT / GSK3β signaling pathway, thereby inhibiting Tau protein hyperphosphorylation and reducing neuroinflammatory responses. Furthermore, it has been confirmed that cyanidin-3-O-rutin glycoside directly binds to acetylcholinesterase, verifying AChE as its key target, providing new natural active compounds and their mechanisms of action for the prevention and treatment of neurodegenerative diseases.

This invention relates to the field of deep processing technology of white hyacinth bean, specifically to a method for preparing a compound spleen-strengthening and dampness-removing white hyacinth bean paste. This invention utilizes low-temperature roasting to pretreat a specific ratio of dampness-removing medicinal herbs, including licorice, poria cocos, coix seed, and bitter almond, and prepares a compound decoction rich in dampness-removing components. This decoction is then used as a biological stressor to promote the germination of white hyacinth beans, with its concentration controlled to encourage moderate germination and the production of substances that resist adverse conditions. Simultaneously, leveraging the plant's water absorption capacity during germination, the invention incorporates the dampness-removing components of the compound decoction. Stable solid-state fermentation with pure Rhizopus further enhances the dampness-removing components of the compound white hyacinth bean and fermented soybean. This not only increases the content of major dampness-removing components such as polyphenols, proteins, and polysaccharides in the white hyacinth bean and fermented soybean, but also increases the content of trace dampness-removing substances such as rutin, quercetin, kaempferol, luteolin, and picolinic acid. It also promotes the dissolution of dampness-removing amino acids—alanine and glycine. The dampness-removing compound white hyacinth bean and fermented soybean components are clearly defined, and the content of spleen-strengthening and dampness-removing substances is richer.

The present application relates to the technical field of fermented food, in particular to a kind of purple red koji fermented coarse grain functional solid drink and its preparation method.The present application is combined with preferred coarse grain raw material, high-activity purple red koji fermentation base material and function-oriented formula design, dry granulation improvement process, successfully developed a kind of purple red koji fermented coarse grain functional solid drink.The product not only gives full play to the significant improvement of purple red koji fermentation on the active ingredients of coarse grain, but also through scientific compounding and process optimization, endows the product with high protein, high resistant starch, high phenolic substance, low fat characteristics, improves the problem of poor brewing property of traditional coarse grain solid beverage, meets the demand of modern consumers for healthy and convenient food.Purple red koji bacteria solid-state fermentation is carried out with tartary buckwheat as matrix, so that the content of total phenol, total flavonoids, rutin and other key active ingredients increases, which is 2597.11 mg / 100g, 2525.39 mg / 100g and 2415.09 mg / 100g respectively, lays a solid functional material foundation for the product.

The application discloses a mulberry leaf and sophora japonica composite beverage and a preparation method thereof, and relates to the food processing technical field. The mulberry leaf juice and the sophora japonica juice are compounded at a high proportion, and combined with fructooligosaccharide, L-malic acid and hydroxypropyl-beta-cyclodextrin to obtain the mulberry leaf and sophora japonica composite beverage. The preparation method is as follows: the mulberry leaves and the sophora japonica are respectively subjected to ultrasonic extraction to obtain the mulberry leaf juice and the sophora japonica juice; the raw materials are mixed, subjected to high-pressure homogenization treatment and ultrahigh-temperature instant sterilization, and then filled to obtain the mulberry leaf and sophora japonica composite beverage. According to the application, the formula composition and the preparation process are optimized, the content of active ingredients such as rutin and DNJ in the composite beverage can be effectively improved, and the composite beverage has good taste. Specifically, the rutin retention rate is 87.3%-90.2%, and the DNJ retention rate is 86.5%-89.4%. In addition, the prepared sophora japonica composite beverage can be stored at room temperature without adding preservatives, and the storage time is not less than 12 months.

The application discloses a tobacco leaf baking quality improvement microbial agent and application thereof, and the active component of the improvement microbial agent is composed of bacillus amyloliquefaciens and bacillus subtilis, and the improvement microbial agent is sprayed on the surface of tobacco leaves before and after harvesting and before baking. Through application of the specific compound microbial agent, the leaf microorganism community in the tobacco leaf conditioning process can be effectively controlled, and beneficial metabolism can be directionally promoted. Application results show that the application can significantly increase the contents of total sugar, reducing sugar, potassium and chlorine of tobacco leaves, selectively increase polyphenol substances such as scopoletin and rutin, enrich aroma components such as linalool and spirorolandane which give woody and floral styles, and improve the softness of tobacco leaves, so that the characteristic quality of honey sweet type tobacco leaves is comprehensively improved.

This invention discloses an α-rhamnosylase mutant and its applications, belonging to the field of microbial engineering technology. The α-rhamnosylase mutant disclosed in this invention has the amino acid sequence shown in SEQ ID NO.2, which consists of a total of 268 amino acid residue point mutations and two frameshift mutations adding 14 amino acid residues based on the parental amino acid sequence of α-rhamnosylase SEQ ID NO.1. Results show that the parental α-rhamnosylase and the α-rhamnosylase IgRha M24-5 mutant enzyme have conversion rates of 72.84% and 99.76% for astragalus root C, respectively; and conversion rates of 90.33% and 99.81% for rutin, respectively. This indicates that the α-rhamnosylase mutant has good application prospects in the selective biotransformation of flavonoid glycosides to prepare derhamnosylflavones under high-temperature conditions.

The present invention relates to the pharmaceutical field. Disclosed are a composition, and a preparation method therefor and the use thereof. Specifically disclosed is a solid composition containing rutin and an amino acid compound. A rutin supersaturated solution that maintains high concentration and long-term stability without precipitation or crystallization can be prepared without altering the chemical structure of rutin. The rutin supersaturated solution can maintain a solution state having high concentration in the gastrointestinal tract without precipitation or crystallization, thereby improving the bioavailability of rutin.

This invention relates to the field of traditional Chinese medicine component analysis technology, specifically to the fingerprint spectrum of Juanxiao tablets and its establishment method. The method for establishing the fingerprint spectrum of Juanxiao tablets includes: using isoharonidin, isovitexin, rutin, hesperidin, lemon balm glycoside, aloe-emodin, nobiletin, and citrus reticulataein as reference standards, and Juanxiao tablets as the test sample; using HPLC to detect the reference standards and the test sample, and then comparing and analyzing them to determine the common characteristic peaks of Juanxiao tablets; wherein the HPLC conditions include: a C18 column, mobile phase A as a nitrile solvent, mobile phase B as a 0.05-0.15% phosphoric acid solution, and gradient elution. This method can effectively detect characteristic peaks, reduce the drying of impurity peaks, and the obtained fingerprint spectrum has high resolution and good peak shape. All characteristic peaks are baseline separated, enabling a more comprehensive and accurate evaluation of the quality of Juanxiao tablets.

This invention discloses a rutin-containing composition and its applications, belonging to the field of drug development technology. Through molecular docking simulations, animal experiments, and cell experiments, this invention demonstrates that rutin exerts its detoxification effect through a dual mechanism: firstly, it antagonizes the inhibitory effect of α-amatoxins on RNA polymerase II, improving transcriptional repression induced by poisoning; secondly, it alleviates oxidative stress caused by α-amatoxins, reducing MDA content and increasing SOD activity. Rutin significantly improves the survival rate of mice poisoned by α-amatoxins, improves hepatocellular pathological damage, and reduces liver function indicators AST and ALT levels. The optimal dosage is 175 mg / kg, indicating that rutin can be considered a potential therapeutic drug for α-amatoxins poisoning. This invention provides a new treatment option for α-amatoxins poisoning. Rutin is a marketed drug with guaranteed safety and significant advantages for clinical translation.

The present application discloses a tobacco polyphenol oxidase NtPPO5, a coding gene thereof, a recombinant expression vector and application. The tobacco polyphenol oxidase coding gene NtPPO5 has a nucleotide sequence of (1) a nucleotide sequence shown in SEQ ID NO. 1; or (2) a nucleotide sequence shown in SEQ ID NO. 1, which is substituted and / or deleted and / or added with one or more nucleotides and expresses a same function protein. It is found through detection that overexpression of the NtPPO5 gene can significantly increase the activity of polyphenol oxidase in tobacco plants; moreover, the tobacco plants overexpressing the NtPPO5 gene have increased internode distance, reduced total leaf and bud number, and can effectively improve the plant type of the tobacco plants; in addition, the NtPPO5 gene overexpression can reduce the contents of polyphenols chlorogenic acid and rutin in tobacco leaves in the maturation period.

The application provides a component extraction and separation method for field tobacco leaves, and the method comprises the following steps: 1) killing green treatment; 2) extracting solanesol; 3) extracting total chlorogenic acid and rutin; 4) separating solanesol; and 5) separating total chlorogenic acid and rutin. The method is used for killing green pretreatment of field tobacco leaves, so that the content of each main component is improved, the purity of solanesol, rutin and total chlorogenic acid obtained in the extraction process is more than 90%, and the utilization value of the active substances is increased.

This invention discloses a method for detecting the content of active ingredients in a lung-heat clearing and detoxifying decoction, comprising the following steps: S1. Accurately weigh the reference standards of morin A, ferulic acid, p-coumaric acid, and rutin, dissolve and dilute them in methanol to prepare a mixed reference solution; S2. Accurately weigh the lung-heat clearing and detoxifying decoction sample, add solvent for extraction, filter or centrifuge the extract, and take the filtrate as the test solution; S3. Detect the sample using high-performance liquid chromatography (HPLC), with the following chromatographic conditions: a C18 column packed with octadecylsilane-bonded silica gel; a mobile phase consisting of methanol as phase A and 0.1% phosphoric acid aqueous solution as phase B, with gradient elution; a detection wavelength of 315 nm for 0-18 minutes and 257 nm for 18-40 minutes; a column temperature of 25-35℃; and a flow rate of 0.8-1.2 ppm. mL / min; S4. Accurately pipette the reference solution and the test solution separately and inject them into the high performance liquid chromatograph for determination. Qualitative identification is performed based on the retention time of the chromatographic peaks, and the content of each component is calculated using the external standard method based on the peak area.

This invention discloses a compound health-preserving wine rich in natural active substances and its preparation method. The health-preserving wine uses high-quality base wine as a carrier, and adds rutin, cordycepin, anthocyanins, polysaccharides, and saponins. It is prepared through a series of processes including raw material pretreatment, ultrasonic-assisted extraction, low-temperature directional extraction, vacuum concentration, and low-temperature compounding. Rutin has antioxidant properties, cordycepin can enhance immune function, anthocyanins have anti-inflammatory activity, polysaccharides contribute to comprehensive health maintenance, and saponins can improve physical strength. Through the synergistic effect of multiple active ingredients, this product can effectively combat aging, prevent and treat hyperlipidemia, hypertension, and hyperglycemia, and has good safety and taste, making it suitable for long-term consumption by health-conscious and sub-healthy individuals. The preparation method provided by this invention is simple, easy to scale up, and has broad application prospects.

This invention discloses the application of Morkotin A (quercetin-3-O-rutin-7-O-glucoside) in the preparation of pharmaceuticals, health foods, or functional foods, belonging to the field of biomedical technology. Animal experiments have demonstrated that Morkotin A can significantly increase bone mineral density in ovariectomized osteoporotic mice, improve bone microstructure, and regulate calcium and phosphorus metabolism and bone turnover marker levels. Simultaneously, in vitro cell experiments have confirmed that Morkotin A can significantly promote osteoblast differentiation and inhibit osteoclast formation. The Morkotin A used in this invention is derived from the medicinal and edible herb, Lycium barbarum leaf, and has high safety. Both in vitro and in vivo experiments have confirmed that Morkotin A promotes bone formation and inhibits bone resorption, making it suitable for development as a drug for the prevention and treatment of osteoporosis, or as a health food or functional food that helps improve bone mineral density.

The drug "Antigrippin R" contains ascorbic acid, rutin, diphenhydramine, calcium gluconate, rimantadine and auxiliary substances in the following ratio of components, g per one tablet weighing 0.5-0.55 g: ascorbic acid 0.05-0.3 calcium gluconate - 0.05-0.1 diphenhydramine - 0.01 -0.02 rutin - 0.01-0.02 rimantadine - 0.025-0.1 auxiliary substances to obtain a tablet. Starch, calcium stearate, talc are used as auxiliary substances.

The application discloses application of NtPPO8 gene in content regulation of tobacco polyphenols and improvement of germplasm resources, and belongs to the technical field of plant genetic engineering. The application uses cDNA of leaves of common tobacco at different periods as a template, and uses PCR technology to obtain a NtPPO8 gene. Through cloning and analysis of the NtPPO8 gene, it is found that overexpression of the gene in tobacco plants can significantly improve the activity of polyphenol oxidase in leaves. The tobacco polyphenol oxidase coding gene NtPPO8 found in the application enriches the tobacco polyphenol oxidase gene family, and lays a foundation for studying the function of polyphenol oxidase in tobacco. Further detection shows that, compared with a normal control group, the content of chlorogenic acid and rutin in a transgenic strain is significantly reduced. The application lays a foundation for clarifying the molecular regulation mechanism of chlorogenic acid and rutin biosynthesis, and provides a new precise regulation gene for synthesis of chlorogenic acid and rutin in tobacco.

This invention discloses the application of the CsFNS1 gene in increasing the flavonoid content of citrus fruits, belonging to the field of agricultural bioengineering technology. It provides the application of the CsFNS1 gene in increasing the flavonoid content of citrus fruits, and the nucleotide sequence of the CsFNS1 gene is shown in SEQ ID NO.1. It also provides a method for increasing flavonoid content in citrus peel using the CsFNS1 gene, comprising the following steps: cloning the citrus CsFNS1 gene; constructing a CsFNS1 overexpression vector; transforming the overexpression vector into Agrobacterium tumefaciens to obtain Agrobacterium tumefaciens bacterial solution; and injecting it into citrus fruits to obtain citrus fruits overexpressing the CsFNS1 gene. This invention provides a new option for increasing flavonoid content in citrus peel, effectively increasing the content of rutin, hesperidin, and / or lemon balm glycosides in citrus peel. This has significant application value for cultivating new functional citrus varieties and accelerating the breeding process.