275 results about "Rutin" patented technology

The invention provides a technical method for improving the anti-aging effect of eurotium cristatum tea compound fermentation liquor, and belongs to the technical field of microorganisms, the Latin name of eurotium cristatum is Eurotium cristatum, the eurotium cristatum is preserved in the China Center for Type Culture Collection, and the preservation number is CCTCC M 2024643. According to the method, the yeast seed solution is inoculated into the tea-sea buckthorn leaf culture solution fermented by the eurotium cristatum, so that the content of anti-aging active components such as procyanidine B2, quercitrin, cyanidin-3-glucoside, nicotinic acid, naringenin and rutin in the fermentation culture solution can be remarkably increased through co-fermentation, and the elastase inhibition rate can be remarkably increased; the anti-aging effect of a component system of the fermentation culture solution is enhanced. The prepared tea-hippophae rhamnoides leaf compound fermentation liquor is suitable for development of anti-aging cosmetics and has a wide market prospect.

The invention discloses bacillus thuringiensis for producing flavonoid components and application thereof, relates to the field of microorganisms, and aims to provide bacillus thuringiensis which is separated from industrial hemp leaves and is preserved in the China Center for Type Culture Collection, the preservation address is Wuhan University, Wuhan, on June 17, 2024, and the preservation number is CGMCC NO. The preservation number of the strain is CCTCC NO: M 20241242. The strain is not only a biological insect-resist agent, but also contains flavonoid compounds, namely rutin and orientin, in metabolites. The strain can be used for producing flavonoid compounds through fermentation.

The present invention belongs to the field of biotechnology, and discloses a composition for reducing the emission of odorous substances from poultry, comprising rutin or mulberry leaf extract, Clostridium butyricum, and Bacillus subtilis; the mass ratio of rutin or mulberry leaf extract, Clostridium butyricum, and Bacillus subtilis is 1-3:0.5-1.5:1; the specification of Clostridium butyricum is 1 billion CFU / g, and the specification of Bacillus subtilis is 1 billion CFU / g. The composition utilizes the flavonoid additive rutin or mulberry leaf extract, Bacillus subtilis, and Clostridium butyricum in combination, which can significantly improve the ability of Bacillus subtilis and Clostridium butyricum to inhibit odor. At the same time, the present invention also discloses the application and feed of the composition.

The invention discloses a titanium-doped lamellar FER molecular sieve and application thereof in a rutin electrochemical sensing detector, and belongs to the technical field of molecular sieve synthesis and electrochemical sensor detection. The titanium-doped lamellar FER molecular sieve is prepared by taking an organic compound as a template agent through a hydrothermal method. The structural formula of the organic compound is NH2-C2H4-NH-(CH2) n-O-(p-C6H4) 2-O-(CH2) n-NH-C2H4-NH2, and the structural formula of the organic compound is NH2-C2H4-NH-CH2. Wherein n ranges from 10 to 16. The preparation method comprises the following steps: immobilizing chitosan on a titanium-doped lamellar FER molecular sieve to obtain a composite material, and modifying an electrode by adopting the obtained composite material to construct an electrochemical sensor; the obtained electrochemical sensor is used for detecting and analyzing rutin. The rutin detection concentration linear range of the obtained electrochemical sensor reaches 0.1-10 [mu] mol / L, the concentration detection range is wide, the detection sensitivity, accuracy, repeatability and selectivity are high, the detection lower limit is low, and the stability is good.

The invention relates to application of an antioxidant liposome in preparation of a medicine for preventing and / or treating inner ear injury caused by active oxygen accumulation, and belongs to the field of medical technologies and biological medical materials. The antioxidant lipidosome is rutin-entrapped lipidosome, and the inner ear injury is preferably cis-platinum induced inner ear injury. The antioxidant liposome disclosed by the invention is used for inhibiting active oxygen accumulation, mitochondrial injury and cell apoptosis in cells; the compound is used for inhibiting auditory hair cell mitochondrial injury in cochlea. The antioxidant liposome disclosed by the invention can be used for effectively packaging medicines and improving the bioavailability and the stability of the medicines; the limitation of a tympanic membrane barrier and other biological barriers can be overcome, so that the medicine can effectively reach the inner ear part; precise targeted delivery of the medicine can be achieved, and it is ensured that the medicine is intensively released at the needed position.

The invention relates to the field of bacteriostatic cream, and particularly discloses skin bacteriostatic cream and a preparation method thereof. The skin antibacterial cream is prepared from the following raw materials in parts by weight: 4 to 6 parts of bran coat extract, 2 to 4 parts of chlorhexidine salt, 2 to 4 parts of rutin extract, 1 to 3 parts of carnosine, 0.5 to 0.8 part of sodium hyaluronate, 0.8 to 1.2 parts of film-forming agent and 5 to 8 parts of purified water. The preparation method comprises the following steps: mixing the film-forming agent and a part of water, and uniformly stirring to obtain a film-forming agent solution; the preparation method comprises the following steps: mixing sodium hyaluronate and water, heating, stirring until the sodium hyaluronate and the water are completely dissolved, adding the cavings extract, the rutin extract, the chlorhexidine salt and the carnosine, stirring until the mixture is uniformly dispersed, heating to 75-85 DEG C, emulsifying for 25-35 minutes, homogenizing, adding a film-forming agent solution into the homogenized emulsion, and stirring, concentrating, degassing and discharging the paste in vacuum to obtain the antibacterial paste. The composition can be used for diminishing inflammation and resisting bacteria of damaged skin, and has the advantages of being high in antibacterial rate and capable of avoiding skin pigmentation.

The invention discloses a screening method of allergen substances in a carthamin yellow injection, a verification method of the allergen substances in the carthamin yellow injection, a detection method of the allergen substances in the carthamin yellow injection and a production process of the carthamin yellow injection. The screening method of the allergen substances in the carthamin yellow injection comprises the following steps: S1, extracting components in the carthamin yellow injection; s2, separating and screening components in the extract by adopting a high performance liquid chromatography-ultraviolet detection-mass spectrum and human serum albumin combined detection technology; and S3, screening out a substance which can be combined with the HSA, and determining the substance as a sensitization hapten substance which has sensitization potential. The invention aims to detect that the 6-hydroxykaempferol-3-rutin-6-glucoside has HAS binding characteristic, hapten attribute and sensitization potential, and can provide basis for process improvement of carthamin yellow injection and reduce the sensitization risk in clinical application.

The invention belongs to the technical field of sympathetic nerve mimetic pharmaceutical preparations, and particularly relates to a stable oxymetazoline hydrochloride preparation and a preparation method thereof. The oxymetazoline hydrochloride preparation is prepared from oxymetazoline hydrochloride, rutin, a pH regulator, a preservative, an isotonic agent and water. According to the invention, the intermolecular interaction of oxymetazoline hydrochloride and rutin is utilized to form a compound system, and the preparation method of the oxymetazoline hydrochloride preparation is optimized, so that the stability of the oxymetazoline hydrochloride preparation is effectively improved.

The invention provides a rutin-modified soybean protein isolate-pectin-genipin nano-particle carrier and a preparation method of the rutin-modified soybean protein isolate-pectin-genipin nano-particle carrier. The preparation method of the nanoparticle carrier comprises the following steps: preparing a soybean protein isolate solution, a soybean protein isolate solution after pH shift, an aqueous solution containing rutin-modified soybean protein isolate and an aqueous solution containing rutin-modified soybean protein isolate-pectin; the aqueous solution contains rutin-modified soy isolate protein-pectin-genipin, the concentration of the genipin is 0.05 mg / mL, and the rutin-modified soy isolate protein-pectin-genipin nano-particle carrier is prepared from the rutin-modified soy isolate protein-pectin-genipin nano-particle carrier. The invention further provides application of the rutin-modified soy isolate protein-pectin-genipin nanoparticle carrier. The rutin-modified soy isolate protein-pectin-genipin nanoparticle carrier is used for sustained release of gastric drugs and targeted release of drugs in small intestines. According to the preparation method disclosed by the invention, a rutin-modified soybean protein isolate-polysaccharide nanoparticle carrier with a shell-core structure can be formed, gastric acid erosion can be effectively resisted, and the rutin-modified soybean protein isolate-polysaccharide nanoparticle carrier is delivered to small intestines in a targeted manner.

The invention discloses a method for converting rutin into quercetin through biological catalysis, and belongs to the technical field of biological catalysis. The method comprises the following steps: mixing rutin and a solvent to obtain a quasi-solid rutin suspension; mixing the paraphlonia plant endogenous enzyme and the quasi-solid rutin suspension for enzymolysis, and then filtering, washing and drying to obtain quercetin; the solvent is methanol with the concentration of 5-50%, ethanol with the concentration of 5-50% or acetone with the concentration of 10-50%. The method is mild in biological catalytic reaction condition, does not generate pollutants such as acid waste liquid, and meets the environmental protection requirement. Efficient conversion of quercetin is realized by adopting a quasi-solid rutin strategy (rutin suspension reaction system), and the problem of substrate solubility limitation is solved. High-purity quercetin can be obtained through simple washing, the product purity is larger than 95%, and food and clinical application can be met.

The application discloses a kind of super tough polyvinyl alcohol composite hydrogel with biological activity and preparation method thereof.The polyvinyl alcohol composite hydrogel refers to that polyvinyl alcohol (PVA) is added into NaOH solution, heated to 90 DEG C, and stirred vigorously for 2 hours, after PVA is completely dissolved, a certain proportion of sodium lignosulfonate (LS) and rutin (RT) powder is added, after sodium lignosulfonate and rutin are completely dissolved, it is air-dried into 0.3~0.5 mm thick film at room temperature, then it is immersed in 20~30 mL 10 wt% NaCl solution and swells for 3~5 hours, to obtain PVA-LS-RT composite hydrogel.The polyvinyl alcohol composite hydrogel prepared by the application has super strong mechanical property, and has higher antibacterial, antioxidant and wound healing capacity, and has good application prospect in wound dressing, artificial cartilage and flexible electronics and other cutting-edge fields.

The invention relates to the technical field of oral liquid, in particular to plant-source skin-tendering oral liquid and a preparation method thereof, and the plant-source skin-tendering oral liquid comprises the following raw materials: an inclusion composition, plant-source composition fermentation powder, pleurotus citrinopileatus concentrated powder, cubilose peptide, sodium hyaluronate, haematococcus pluvialis powder and distilled water. According to the invention, the emblic leafflower fruit extract is encapsulated by using the abelmoschus esculentus pectin extract-gelatin complex, so that the emblic leafflower fruit extract is tightly wrapped in the inclusion composition, and the inclusion composition is beneficial to retaining the active ingredients of the emblic leafflower fruit extract, is more beneficial to reducing the generation of AGEs, and covers the bitter taste of the emblic leafflower fruit extract; the drinking taste of the plant source skin tendering oral liquid is improved; according to the present invention, the plant source composition is subjected to fermentation treatment, such that the synthesis and the accumulation of the rutin, the quercetin and the kaempferol in the plant source composition can be promoted, such that the plant source composition fermentation powder obtained after the fermentation treatment can easily reduce the generation of AGEs, and can enhance the anti-saccharification and anti-aging effects.

The invention belongs to the technical field of natural plant extracts, and particularly relates to a preparation method, a product and application of an antioxidant repair rosemary extract. According to the technical scheme, snail protease and bromelain are added during rosemary leaf pretreatment, so that cell walls of rosemary leaves are fully destroyed, and release of intracellular active ingredients can be effectively promoted. According to the technical scheme, an extraction solvent A and an extraction solvent B are used, the extraction solvent A is mainly used for extracting flavonoid substances such as rutin, hesperetin and thistle flavin in the rosemary, and the extraction solvent B is mainly used for extracting terpene components such as rosmarinic acid, carnosic acid and rosmarinol in the rosemary. According to the technical scheme, the rosemary extract is obtained by mixing and extracting the extract A and the extract B, and it is found through experimental verification that the extract has remarkable effects in the aspects of oxidation resistance, soothing and skin repairing.

The invention discloses an alpha-rhamnosidase mutant and application thereof, and relates to the technical field of microbial engineering. The amino acid sequence of the alpha-rhamnosidase mutant disclosed by the invention is as shown in SEQ ID NO.2, and the alpha-rhamnosidase mutant has 268 amino acid residue point mutation in total on the basis of the amino acid sequence SEQ ID NO.1 of an alpha-rhamnosidase parent, and 14 amino acid residues are increased by 2-segment frameshift mutation. The results show that the conversion rates of the alpha-rhamnoside parent enzyme and the alpha-rhamnoside IgRha M24-5 mutant enzyme to the epimedin C are respectively 72.84% and 99.76%; and the conversion rates of rutin are respectively 90.33% and 99.81%. Therefore, the alpha-rhamnosidase mutant has a good application prospect in the aspect of preparing rhamnosylated flavonoids by selectively biologically converting flavone glycoside compounds under a high-temperature condition.

The present invention relates to an antioxidant and anti-inflammatory composition comprising a carrot leaf lava seawater extract as an active ingredient. The carrot leaf lava seawater extract of the present invention has an increased total polyphenol, rutin, and caffeic acid content compared to a carrot leaf water extract, has excellent DPPH radical scavenging activity, and has a remarkable effect of inhibiting NO production. Therefore, the carrot leaf lava seawater extract of the present invention can be useful as an antioxidant and anti-inflammatory composition.

The invention provides a quality detection method of antiviral particles. The quality detection method adopts HPLC-MS / MS (High Performance Liquid Chromatography-Mass Spectrometry / Mass Spectrometry) to simultaneously determine the following components: forsythiaside A, patchouli ketone, verbascoside, mangiferin, 5-methoxy-8-hydroxypsoralen, curcumenol, luteolin-7-glucuronide, kaempferol-3-O-rutinoside, baicalin, rutin and bergamot phenol. The detection method is a quantitative determination method for 11 active components in the antiviral particles, the detection method is short in time and low in cost, the quantitative analysis method is expected to provide reference for quality control of the antiviral particles, and the clinical curative effect and safety of the antiviral particles are guaranteed.

The invention relates to the technical field of analytical chemistry and food pharmacy detection, in particular to a rutin antioxidant activity colorimetric determination method based on a composite nano enzyme, which comprises the following steps: mixing a to-be-detected sample solution with an ABTS free radical cation working solution to carry out first reaction, so that an antioxidant substance in a sample and ABTS free radical cations are subjected to scavenging reaction; adding the composite nano enzyme, a 3, 3 ', 5, 5'-tetramethyl benzidine solution and a hydrogen peroxide solution into a system after the first reaction is completed, and initiating a second reaction; and measuring the absorbance value of the system after the second reaction under a specific wavelength, comparing the absorbance value with the standard curve obtained through the treatment in the same step, and calculating the antioxidant activity expressed by the equivalent concentration of rutin in the sample to be detected. By optimizing reaction conditions, the method provided by the invention simplifies operation steps and can provide accurate measurement results in a wider concentration range.

The invention relates to the field of medicines, discloses a composition as well as a preparation method and application thereof, and particularly relates to a solid composition containing rutin and amino acid compounds, which can be used for preparing a rutin supersaturated solution which is high in concentration, is kept stable for a long time and cannot separate out crystals on the premise of not changing the chemical structure of rutin. The rutin supersaturated solution can keep a high-concentration solution state in a digestive tract without separating out crystals, so that the bioavailability of rutin is improved.

The invention relates to the technical field of health food, in particular to a composition and application thereof in maintaining the healthy level of blood sugar. The composition comprises rutin, quercetin, kaempferol and pectin. Experiments show that the composition obviously reduces the activity of alpha-glucosidase and obviously delays the digestibility of starch, so that the postprandial blood sugar level of mice is reduced, and the effect of the composition is obviously superior to that of other flavonoid and pectin compositions. It is shown that after the rutin, the quercetin, the kaempferol and the pectin are compounded according to a certain proportion, an obvious synergistic effect is generated. Besides, according to the composition prepared by the invention, the dosage of quercetin is reduced, the utilization rate of pectin is increased, the production cost is reduced, the raw materials are pure natural, safe and non-toxic, the functionality is strong, the cost is low, and the composition has a wide application prospect in the aspect of deep processing of starch-based healthy foods.

The invention discloses a determination method of an Ajowan fruit fingerprint spectrum and the fingerprint spectrum thereof, and relates to the technical field of fingerprint spectrums of medicinal materials. The method comprises the following steps: taking rutin, caffeic acid, rosmarinic acid and p-coumaric acid as reference substances, dissolving the reference substances in a solvent, and fixing the volume to obtain a reference substance solution; the method comprises the following steps: taking different batches of ajowan fruit medicinal materials as test products, adding a solvent into the test products, extracting, filtering, and taking subsequent filtrate to obtain a test product solution; respectively injecting the reference substance solution and the test solution into a liquid chromatograph by utilizing a high performance liquid chromatography, recording a chromatogram, and constructing the fingerprint spectrum of the Ajowan fruit according to the chromatogram; and determining the component content of the test solution through an external standard method according to the chromatogram. According to the method, the quality of the ajowan fruit medicinal material is controlled by adopting a method of combining chemical component content determination and a fingerprint spectrum, so that the effective and comprehensive control on the ajowan fruit medicinal material is realized, and a basis is provided for the quality control standard of the ajowan fruit medicinal material.

The application relates to a method for hydrothermal acid-controlled degradation of mulberry leaf polysaccharide and antioxidant application thereof, and belongs to the technical field of extraction of active ingredients in natural products. The method comprises the following steps: pretreating raw materials, hydrothermally reacting and acid-catalytically treating mulberry leaves, and alcohol-sedimenting, separating and purifying mulberry leaf polysaccharide, so as to obtain mulberry leaf polysaccharide, finally, the antioxidant application of the mulberry leaf polysaccharide is explored, and a theoretical basis is provided for development of mulberry leaf resources. The method can not only obtain mulberry leaf polysaccharide, but also obtain high-value natural active ingredients such as rutin, astragalin, scopoletin and isoquercitrin in the mulberry leaves, is beneficial to development and utilization of the mulberry leaf resources, meets the needs of people's health, and has practical significance and economic value.

The invention discloses a rutin-derived compound as well as a preparation method and application thereof. The rutin-derived compound has the following structural general formula, according to the present invention, the rutin is adopted as the raw material to prepare the rutin derivative through the multi-step reaction, and the research results show that the rutin derivative can provide the uric acid reducing effect in the short time, and the elimination effect on the blood uric acid at the low dose concentration is even superior to the elimination effect on the blood uric acid of the positive drug benzbromarone, such that the high clinical application value is provided; and # imgabs0 #.

The invention relates to the field of cosmetics, in particular to a two-way fermentation method, a litchi processing byproduct fermentation product and cosmetics. Comprising the following steps: inoculating a schizophyllum commune strain into a culture medium for fermentation; the culture medium comprises litchi processing by-products and saccharides; the litchi processing byproducts comprise litchi shells and / or litchi seeds; wherein in the culture medium, the mass ratio of the litchi processing byproducts to the saccharides is (1%-8%): (0.4%-2%). According to the technical scheme, the schizophyllum commune is used as an original strain to ferment the litchi processing byproducts, so that the schizophyllum commune can be promoted to secrete the lyophyllum commune polysaccharide SPG, and the release amount of rutin in the litchi processing byproducts can be effectively increased. Meanwhile, the rutin and the lyophyllum commune polysaccharide SPG are stably combined, and the rutin and the lyophyllum commune polysaccharide SPG can stably exist without additional polyhydric alcohols. The prepared fermentation product rich in SPG and rutin not only has the oxidation resistance of rutin, but also achieves the anti-inflammatory and anti-aging effect.

The invention relates to application of rutin in an anti-coxsackie virus drug, and relates to the field of drugs. Rutin can be highly and stably combined with a CVB5-3C protein target through Van der Waals force and hydrogen bonds, replication of the coxsackie virus is inhibited, and the effect of treating and / or preventing the coxsackie virus is achieved.

An object of the present invention is to provide an excellent vascular endothelial function improving agent containing hesperidin and rutin. The present invention includes a vascular endothelial function improving agent containing alpha-glucosyl hesperidin and alpha-glucosyl rutin.

The application discloses a preparation method of a hawthorn extract for cigarettes and application of the hawthorn extract in cigarettes, and the preparation method comprises the following steps: mixing and sterilizing rutin with water; inoculating Pseudomonas acidovorans ZY-03, Bacillus subtilis SMXP-58, Micrococcus ZY02 and Pichia membranacei into culture media respectively to form seed liquids; inoculating the seed liquids of the Pseudomonas acidovorans ZY-03 and the Bacillus subtilis SMXP-58 into the rutin solution to obtain a fermentation product 1; grinding and crushing hawthorn and mixing the hawthorn with water and heating, then filtering and mixing the hawthorn with the fermentation product 1 to obtain a mixed solution; mixing the four seed liquids and inoculating the mixed solution into the mixed solution; after fermentation, the mixed solution is extracted by refluxing with an organic solvent, centrifuged, rotary evaporated, and added with an organic reagent to obtain the hawthorn extract for cigarettes. The rutin and the hawthorn are mixed and fermented by using microorganisms, the fermentation effect is improved, flavonoids such as rutin and macromolecular substances in the hawthorn are degraded and converted into aroma components, and the quality of the hawthorn extract for cigarettes is improved.

The invention discloses application of rutin hydrate in preventing and treating repeated embryo planting failure, and belongs to the technical field of assisted reproduction. It is found for the first time that CD36 is a key membrane protein for lipid transport and uptake of EECs, rutin hydrate competitively inhibits combination of CD36 and natural ligands of CD36, abnormal lipid excessive uptake is remarkably blocked, lipid accumulation and lipid toxicity damage in cells are effectively relieved, and the effect of inhibiting EECs lipid transport and uptake is achieved. And finally, the endometrial receptivity defect related to RIF is reversed, and the embryo adhesion and implantation capability is remarkably improved. Therefore, rutin hydrate provides a brand new strategy for developing targeted therapy drugs for RIF, especially for lipid metabolism disorder type endometrial receptivity badness, and has remarkable clinical application and transformation prospects.

The invention relates to the technical field of biology, in particular to a tobacco Nt4CL9 gene and application thereof. According to the application, tobacco Nt4CL9 is knocked out, and compared with a wild type, the cold stress tolerance of the knocked-out mutant strain is improved, the content of flavonoid substance rutin is obviously increased, and phenolic acid substances are increased; potential support is provided for improvement of tobacco cold stress capacity and tobacco quality, and the method has important practical value in genetic improvement of tobacco important characters and basic research of botany.

The present application relates to a kind of preparation method of Fugunkang composition and its detection method, the preparation method is cut into section, first using red koji and saccharomyces cerevisiae combined fermentation extraction, dregs is extracted with water before boiling, concentrate and extract the fermentation liquor and water, it is obtained.The metabolic function of microorganism is fully utilized, promote the release and absorption of effective components of traditional Chinese medicine in vivo, so as to improve the curative effect of traditional Chinese medicine, reduce the side effect of traditional Chinese medicine.The detection method includes spray agent in just bed, parsnip medicine thin layer identification and the content detection of rutin, hypericin;The thin layer identification can identify just bed and parsnip medicine under the same chromatographic condition, the content detection increases the content detection of hypericin on the basis of original, and the content of rutin and hypericin can be determined simultaneously, save the detection cost, ensure the quality of raw material and finished product.

The invention discloses application of geum aleppicum total triterpenes in treatment of hypertension and a preparation method of the geum aleppicum total triterpenes, the geum aleppicum total triterpenes are obtained by step-by-step extraction and purification of geum aleppicum, and the geum aleppicum total triterpenes comprise bitter raspberry sweet F1, 2-hydroxyl oleanolic acid, 2-hydroxyl ursolic acid, 20beta, 20beta, 20beta, 20beta, 20beta, 20beta, 20beta, 20beta, 20beta The traditional Chinese medicine composition is prepared from the following raw materials in percentage by weight: 28-epoxy-28-hydroxytaxasan-3 beta-ol (taraxane triterpenes), ursolic acid, maslinic acid, rosa multiflora acid and tormentic acid. According to the present invention, the geum aleppicum total triterpenes are extracted and purified from the geum aleppicum step by step, and the geum aleppicum total triterpenes can significantly increase the total cross-sectional area of the vascular bed of the ischemic tissue or organ, such that the low blood pressure can meet the blood perfusion for maintaining the life activity so as to achieve the blood pressure lowering purpose; the compound can be widely applied to the field of hypertension prevention and treatment medicines.