105 results about "Hesperidin" patented technology

The invention discloses an application of a CsHCT1 gene in increasing the content of citrus flavonoids, belongs to the technical field of agricultural biological genetic engineering, and provides an application of the CsHCT1 gene in increasing the content of citrus flavonoids, and the nucleotide sequence of the CsHCT1 gene is as shown in SEQ ID NO.1. The invention further discloses an application of the CsHCT1 gene in increasing the content of citrus flavonoids. The invention also discloses a method for improving the flavone compound of the citrus peel by using the CsHCT1 gene. The method comprises the following steps: cloning the citrus CsHCT1 gene; a CsHCT1 overexpression vector is constructed; transforming the overexpression vector to agrobacterium tumefaciens to obtain an agrobacterium tumefaciens liquid; and injecting the CsHCT1 gene into the citrus fruit to obtain the CsHCT1 gene overexpressed citrus fruit. A new choice is provided for increasing the flavonoid compounds in the citrus peel, the content of vicenin-2 and / or hesperidin in the citrus peel is effectively increased, and the application has great application value for cultivating new functional citrus varieties and accelerating the breeding process.

The invention belongs to the field of pharmaceutical analysis, and discloses a method for simultaneously determining the contents of tolperidone hydrochloride, methyl hesperidin and vitamin B6 in Naoluotong capsules, the method comprises three steps of preparation of a mixed reference solution, preparation of a test solution and content determination, and the chromatographic conditions of the content determination are as follows: a C18 chromatographic column, a C18 chromatographic column, a C18 chromatographic column, a C18 chromatographic column, a C18 chromatographic column, a C18 chromatographic column, a C18 chromatographic column, a C18 chromatographic column and a C18 chromatographic column; a mobile phase is methanol (A)-0.1% glacial acetic acid solution (B), gradient elution is carried out for 0-5 min, and 96% of B is obtained; 5-6 min, 96%-60% of B is selected; 6-20 min, the content of B is 60%-50%; the volume flow rate is 1.0 ml / min; the column temperature is 30 DEG C; the detection wavelength is 291 nm; the sample size is 1 microliter. According to the method, synchronous detection of three components (tolperidone hydrochloride, methyl hesperidin and vitamin B6) with remarkable physicochemical property difference can be realized, the chromatographic condition is simple, and the detection speed is high.

The invention discloses a plant-sourced anti-allergy soothing composition and application thereof, and belongs to the field of cosmetic processing.The plant-sourced anti-allergy soothing composition is prepared from hydrolyzed corn starch, saururus chinensis extract, carrot root extract and water-soluble hesperidin. Active ingredients of plant sources are deeply studied, and the anti-allergy soothing composition has the advantages that the plant-sourced anti-allergy soothing composition is prepared from the hydrolyzed corn starch, the saururus chinensis extract, the carrot root extract and the water-soluble hesperidin; a series of plant extracts with a synergistic effect are screened out, the plant-source multi-dimensional composition for relieving itching and repairing red is successfully researched and developed, and through the synergistic effect of the hydrolyzed corn starch, the saururus chinensis extract, the carrot root extract and the water-soluble hesperidin, the composition has the effects of relieving and repairing skin in a multi-dimensional mode, relieving itching and repairing red. In addition, the composition can repair the skin barrier for a long time and improve the tolerance of the skin, and meanwhile, the composition has good safety and stability and is suitable for various skin types, especially sensitive skin.

The invention provides a donkey-hide gelatin solid beverage and a preparation method thereof, the preparation method comprises the following steps: adding 8 times by mass of water into rhizoma polygonati, Chinese wolfberry, astragalus membranaceus, codonopsis pilosula, red dates, angelica sinensis and orange peel, soaking for 0.5 h, extracting for 2 h for the first time, adding 6 times by mass of water for extracting for 1.5 h for the second time, combining filtrates, and concentrating until the relative density is 1.23 g / cm < 3 > to obtain a water extraction concentrated solution; mixing the water extraction concentrated solution with maltodextrin, and drying to obtain an extract; and adding auxiliary materials into the extract and the donkey-hide gelatin, and molding to obtain the donkey-hide gelatin solid beverage. In the extraction step, proper soaking time, water adding amount and extraction time are combined, so that the ferulic acid content, the total polysaccharide content, the hesperidin content and the calycosin-7-glucoside content in the concentrated solution are all higher. And on the basis, subsequent drying and forming are carried out, so that the final donkey-hide gelatin solid beverage also has relatively high content of the components, and the content in each batch of products is stable.

This invention discloses a quality control method for an anti-snoring oral liquid and its application, belonging to the field of traditional Chinese medicine quality testing technology. The method includes thin-layer chromatography identification of Gleditsia sinensis thorns, Scrophularia ningpoensis, and Glycyrrhiza uralensis, as well as high-performance liquid chromatography (HPLC) determination of hesperidin content. By optimizing pretreatment, developing solvent, and colorimetric conditions, this invention achieves efficient and specific identification and quantification of multiple components in the compound preparation. The method is highly specific, produces clear spots, has good separation, and is tolerant to a wide temperature and humidity range (2-40℃, 20%-75%RH), requiring no strict environmental control, significantly improving detection efficiency and providing a reliable basis for establishing quality standards for this preparation.

The application provides a kind of zhangtou red pericarpium citri reticulatae UPLC quantitative fingerprint detection method, belongs to the quality detection technical field of traditional Chinese medicine.The detection method is prepared by reference solution, preparation of test solution, using ultra high performance liquid chromatography analysis, multiple batches of liquid chromatogram is introduced into "traditional Chinese medicine chromatographic fingerprint similarity evaluation system", 21 common peaks are identified, similarity matching is carried out with 21 common peaks, and the control spectrum of zhangtou red pericarpium citri reticulatae is generated.On this basis, combined with hesperidin, sweet orange flavone, chuan chenpisi, tangeretin, 5-dimethyl chuan chenpisi 5 flavonoid content determination, zhangtou red pericarpium citri reticulatae quantitative fingerprint is established.The application realizes that fingerprint detection and the content determination of 5 flavonoids of zhangtou red pericarpium citri reticulatae can be completed at the same time through one analysis, can quickly, comprehensively and accurately evaluate the quality of zhangtou red pericarpium citri reticulatae, has good reproducibility and stability, and can provide technical support for the uniformity and stability of zhangtou red pericarpium citri reticulatae quality.

The invention discloses a method for identifying Xinhui pericarpium citri reticulatae and pericarpium citri reticulatae, which is characterized in that four active ingredients including hesperidin, nobiletin, tangeretin and 2-methylamino methyl benzoate are simultaneously detected through high performance liquid chromatography, and distinguishing is realized according to detection data. The method comprises the following specific steps: preparing a mixed reference solution and a test solution, extracting a test with methanol, performing ultrasonic treatment, and filtering, specific chromatographic conditions are adopted for detection, the detection wavelength of the hesperidin is 283nm, and the detection wavelengths of the other three components are 330nm. Test results show that the contents of the four active ingredients in the Xinhui pericarpium citri reticulatae are high, the content of methyl 2-methylaminobenzoate is not lower than 0.020%, the ratio of the total amount of nobiletin and tangeretin to hesperidin is not lower than 0.17, and related indexes of the pericarpium citri reticulatae are remarkably low. The method is efficient, accurate, stable and reliable, and provides technical support for standardizing market circulation, guaranteeing product quality and promoting standardized development of the Xinhui pericarpium citri reticulatae industry.

The present invention discloses a method for constructing a fingerprint of Gualou Niu Bang decoction, relating to the technical field of analytical chemistry. The method is simple and has high precision, stability, and repeatability. Determination revealed that seven components, including geniposide, liquiritin, hesperidin, arctiin, baicalin, melaleucalyptol A-7-O-β-D-glucuronide, and wogonin, can be used as common characteristic peaks in the fingerprint of Gualou Niu Bang decoction for identification research.

The application discloses a suspension for improving DON-caused piglet intestinal toxicity and a preparation method and application thereof, and belongs to the technical field of feed additives, and comprises the following raw materials: hesperidin 40-60 mg / mL, sodium butyrate 50-150 mg / mL and PR-39 antibacterial peptide 10-15 mg / mL. The suspension provided by the application can also regulate intestinal flora balance, promote the proliferation of beneficial bacteria such as lactobacillus, and inhibit the growth of harmful bacteria. The effect of the suspension provided by the application is significantly better than that of single component or partial combination, and the suspension provides an efficient and safe nutritional intervention strategy for preventing and treating animal body damage caused by feed mycotoxin pollution.

The utility model provides a foreign matter detecting and removing device used before hesperidin finished product packaging, and belongs to the technical field of hesperidin processing. Comprising a conveying channel, materials are conveyed in the conveying channel through negative pressure suction, a foreign matter detector and a filtering assembly are arranged on the conveying channel, the filtering assembly comprises a filtering channel, a movable cylinder slides in the filtering channel, the movable cylinder comprises a partition plate and filtering cylinders coaxially and rotatably arranged on the two sides of the partition plate, and filtering pieces are arranged on the filtering cylinders; and a collecting assembly for collecting impurities filtered out by the filtering piece is arranged on the filtering channel. Impurities in hesperidin powder passing through the conveying channel can be filtered out through the filtering piece, when the foreign matter detector detects that the impurities pass through, the movable barrel is controlled to move in the filtering channel in a delayed mode, the other filtering barrel is moved into the conveying channel alternately, the filtering barrel with the impurities dumps and collects the impurities through rotation, and therefore the impurities can be filtered out through the filtering piece. The hesperidin powder filtering device has the effect of filtering hesperidin powder under the condition that equipment is not stopped.

The utility model relates to the technical field of extraction equipment, in particular to a hesperidin extraction device which comprises a support, a stand column, a heat preservation barrel and a reaction barrel. A stand column is connected to the middle of the upper surface of the support, a heat preservation barrel is connected to one side of the stand column, and a reaction barrel is connected to the inner wall of the heat preservation barrel. According to the hesperidin extraction device, water in the water tank is fed into the steam generator to generate steam by starting the water pump, the steam is fed into the heat preservation barrel to heat materials in the reaction barrel, then the steam is fed into the water tank from the heat dissipation pipe to preheat the water in the water tank, and then the hesperidin extraction device is heated; materials are fed into the filtering barrel by opening the discharging valve, the driving motor is started to filter the materials through the filtering barrel, the materials are stored in the liquid collecting barrel, separated liquid is taken out by opening the valve, then the liquid collecting barrel and the heat preservation barrel are staggered, the remaining materials are poured out through the filtering barrel, and then the hesperidin extraction device is convenient for solid-liquid separation.

The invention discloses a microbial fertilizer for treating citrus huanglongbing and a preparation method of the microbial fertilizer, and belongs to the field of agricultural biological control. 33 to 50 percent of sodium alginate-chitosan microspheres; 5 to 8% of hesperidin; 3-5% of nano zinc oxide; 2-4% of humic acid chelated boron; 2-4% of sucrose fatty acid ester; 2-4% of diatomite; wherein the preparation method of the sodium alginate-chitosan microspheres comprises the following steps: mixing sodium alginate with a microbial suspension, dropwise adding the mixture into a calcium chloride solution by using an injector, and standing and curing to form a microsphere core; and immersing the cured microspheres into a chitosan solution with the pH value of 6.0, taking out, and freeze-drying to obtain finished microspheres. Wherein the microbial suspension comprises trichoderma, bacillus and actinomycetes. The invention solves the problems of poor prevention and treatment effect of the existing Candidatus Liberobacter asiaticum, difficulty in colonization of the strain at phloem, short survival time and lack of targeted slow release function.

The utility model provides an operation table for hesperidin raw material pretreatment, and belongs to the technical field of hesperidin production. Comprising an operation table body, a cleaning tank is arranged on the operation table body, a liquid discharging pipe is arranged at the bottom of the cleaning tank, a valve is arranged on the liquid discharging pipe, a mounting frame is vertically and slidably arranged in the cleaning tank, a cleaning barrel used for containing hesperidin raw materials is rotatably arranged in the mounting frame, a feeding opening is formed in the outer wall of the cleaning barrel, and a cover plate is rotatably arranged at the feeding opening; a plurality of water inlet holes are formed in the outer wall of the cover plate and the outer wall of the cleaning barrel, a connecting piece is arranged between the cover plate and the cleaning barrel, a spreading and airing frame used for moving to the position below the cleaning barrel to receive hesperidin raw materials is horizontally and linearly arranged on the operation table body in a sliding mode, and a first driving piece used for driving the mounting frame to move is arranged on the operation table body. The second driving part is used for driving the airing rack to move, and a third driving part used for driving the cleaning barrel to rotate is arranged on the mounting frame. And the effect of reducing the labor intensity of workers is achieved.

The invention relates to a method for preparing trilobatin by taking hesperidin as a raw material, which comprises the following steps of: 1, hydrolyzing the hesperidin with the content of more than 90 percent as the raw material to remove rhamnose to obtain an intermediate 1; step 2, reacting the obtained intermediate 1 with p-hydroxybenzaldehyde to obtain an intermediate 2; 3, the obtained intermediate 2 is subjected to a reduction reaction and then purified, and the product trilobatin is obtained. The hesperidin is used as the main raw material to prepare the trilobatin, the hesperidin serving as the raw material is sufficient in source and low in cost and meets market requirements and has competitive advantages, and the preparation method is easy to operate and suitable for industrial production.

Nutritional supplements and compositions for activating the GLP-1 peptide and / or other hormones in a subject in order to regulate blood sugar, weight, and hunger of the subject are described in this application. The compositions include a first nutritional supplement containing specific amounts of hydrolyzed Acacia gum, lemon bioflavonoids, chromium, Berberis aristate, and hesperidin. The compositions also include a second nutritional supplement containing specific amounts of flax seed oil, resistant potato starch, resistant tapioca fiber, kombucha, Bacillus coagulans, Bacillus clausii, and allulose. A third composition includes the first and second nutritional supplements containing these ingredients in sufficient amounts to regulate the GLP-1 peptide and / or other hormones. These compositions are able to increase the GLP-1 concentration in the blood by up to about 140% without binding directly to any GLP-1 receptors. Other embodiments are also described.

The utility model discloses an extraction tank for preparing methyl hesperidin. The extraction tank comprises an extraction tank, a fixed seat is arranged at the top of the extraction tank, a motor and a stirring shaft are arranged on the fixed seat, and one end of the stirring shaft penetrates through a bearing on the fixed seat and is connected with the motor; a paddle wheel and a stirring frame are arranged on the stirring shaft, the stirring frame is provided with lantern rings, transverse rods, vertical rods and stirring blades, the three lantern rings are sequentially arranged on the stirring shaft at intervals, each lantern ring is provided with two transverse rods which are symmetrically arranged, the two vertical rods are connected with the transverse rods on the two sides of the lantern ring respectively, and the stirring blades are fixed to the vertical rods. The paddle wheel at the tail end of the stirring shaft can push liquid to the bottom of the tank, the stirring blades facing the outer side on the vertical rods can stir the liquid outwards, the stirring blades facing the inner side on the vertical rods can stir the liquid outwards, the front end openings of the stirring blades are large, the rear end openings of the stirring blades are small, and the stirring effect is achieved through the flow velocity difference of the liquid at the two end openings. The liquid is stirred in multiple directions, so that the stirring efficiency is improved.

The invention discloses a preparation method of a hesperidin regulated polyamide nanofiltration membrane. The preparation method comprises the following steps: S1, contacting the surface of a base membrane with a water-phase solution, standing, and removing the water-phase solution; s2, the surface of the base membrane with the water phase solution removed in the step S1 makes contact with an organic phase solution, interfacial polymerization is carried out, and the hesperidin-regulated polyamide nanofiltration membrane is prepared; the aqueous phase solution contains hesperidin, an amine monomer and water; the mass volume ratio of the hesperidin to the water is (5-10): 1g / L, and the mass volume ratio of the amine monomer to the water is (4-6): 1g / L; the organic phase solution contains an acyl chloride monomer and an organic solvent; the mass volume ratio of the acyl chloride monomer to the organic solvent is (1.3-2): 1g / L. The polyamide nanofiltration membrane has excellent inorganic salt / antibiotic selectivity while keeping excellent water flux and antibiotic rejection rate, and can be applied to devices such as sewage treatment equipment, seawater desalination equipment, industrial wastewater treatment and recycling equipment and the like.

The present application provides an herbal supplement and method for alleviation of symptoms of gastroesophageal reflux disease (GERD). The herbal supplement comprises two or more herbal components selected from the group consisting of atractloydes extract, dandelion root extract, noni extract, hesperidin extract and quebracho extract.

The invention relates to the technical field of citrus processing, in particular to a composite green extraction technology for low-ester pectin, hesperidin and synephrine in young citrus fruits, which comprises the following steps: (1) crushing the dried young citrus fruits, putting the young fruit powder into an ultrasonic extraction tank, adding distilled water according to the material-liquid ratio of 1: 5-1: 30 g / mL, performing ultrasonic extraction for 20-60 minutes at the ultrasonic frequency of 20-80 kHz, and filtering to obtain filtrate; the temperature is 50-90 DEG C, centrifuging after the treatment is completed, taking supernate, and using residual filter residues for extracting hesperidin and synephrine. According to the technical scheme provided by the invention, composite extraction of low-ester pectin, hesperidin and synephrine in the young citrus fruits can be realized, and the extraction efficiency of functional active substances in the young citrus fruits is effectively improved; and the used extraction solvent has small pollution, is more environment-friendly compared with dichloromethane, ethyl acetate, methanol, acetone and other organic solvents used in the prior art, realizes reutilization of the extraction solvent, reduces environmental pollution, and reduces the extraction cost.

The invention discloses a preparation method and application of an enzyme preparation for enzymatically hydrolyzing bitter substances in citrus juice. Using Aspergillus tabingensis UA13 isolated in the early stage of the laboratory and mutagenized by atmospheric and room temperature plasma (ARTP) as the starting strain, limonin in lemon peel powder is used as an inducer to induce enzyme production, and the fermentation enzyme production process of the strain and the substrate affinity of the enzyme are studied. The results show that: in a 1 L fermentor medium, under the optimal conditions of limonin addition amount of 4.0 mg / L, inoculation amount of 10% (v / v), initial pH value of 5.0, stirring speed of 250 rpm, and fermentation at 30 °C, the highest limonin debittering enzyme activity can reach 48.52 U / mL. The results of the study on the substrate affinity of the enzyme for limonin show that the Km value of the limonin debittering enzyme for limonin is 0.622 mmol / L, indicating strong affinity. At the same time, the enzyme can still efficiently hydrolyze other bitter substances such as naringin and hesperidin, has good heat resistance and acid resistance, and has very good application effects in the citrus juice processing industry.

The invention discloses a preparation method of natural product co-assembled nanoparticles, and hesperidin and emodin are subjected to a co-assembly process to form carrier-free nanoparticles, so that the natural product co-assembled nanoparticles are obtained. The invention further provides the natural product co-assembled nanoparticles prepared by the preparation method and application of the natural product co-assembled nanoparticles. According to the natural product co-assembled nanoparticles provided by the invention, emodin which is rapidly released in an early stage can rapidly kill methicillin-resistant staphylococcus aureus (MRSA) on the surface of a wound, and a local antibacterial barrier is established; the hesperidin continuously released in the later stage plays an anti-inflammatory role by regulating and controlling the expression of inflammatory factors, and promotes the proliferation of fibroblasts and the secretion of angiogenesis-related growth factors (such as VEGF) at the same time. The'space-time synergistic release strategy 'provides a multifunctional platform integrating antibiosis, anti-inflammation and tissue repair for chronic wound treatment, and is suitable for accelerating healing of chronic wounds such as infection, diabetes and burn.

The invention discloses a lactobacillus mucosa mucus strain for producing various active small molecules by fermenting white truffle and cooperatively resisting skin cell aging at multiple targets, and belongs to the technical field of microorganisms. According to the lactobacillus mucosa mucosae CCFM1495 obtained through screening, cell proliferation can be carried out while the white truffle is fermented, and multiple active small molecule substances such as m-hydroxyphenylacetic acid, salicylic acid and hesperetin are generated. The fermentation liquor of the lactobacillus mucosa mucus CCFM1495 fermented white truffle can improve the skin quality through multi-target synergistic resistance to skin cell aging, and has a huge application prospect in preparation of products for improving the skin quality.

The present application relates to the field of pharmaceutical wastewater treatment technology, and discloses a system for treating pharmaceutical wastewater derived from Citrus aurantium, comprising a pretreatment unit, wherein wastewater enters the pretreatment unit, and colloidal proteins in the wastewater are removed by flocculation, and the generated flocculated impurities are formed into agglomerates, and the impurities in the wastewater are filtered by the agglomerates; a molecular imprinting treatment unit, which receives the wastewater output by the pretreatment unit, and adopts Fe3O4@SiO2 core-shell adsorption material with hesperidin as a template as an adsorbent, and cooperates with magnetic field fluidization to carry out targeted absorption of flavonoids in the wastewater, and cooperates with magnet assistance and regeneration process to complete the recovery of flavonoids and regeneration of adsorbent; and a bioelectrochemical reaction unit. Through the synergistic action of multiple mechanisms, the system realizes the step-by-step decomposition and mineralization of complex organic matter, especially showing excellent removal effect on pollutants such as macromolecular drug residues and pigments that are difficult to treat by traditional processes, ensuring that the effluent water quality is stable and meets the standards.

The utility model relates to the technical field of hesperidin extraction and production devices, and particularly discloses a centrifugal device for hesperidin crystallization and separation, which comprises a bearing component arranged in a centrifugal machine body; the lifting assembly is arranged on the cover plate and used for driving the bearing component to do lifting motion in the gravity direction so as to drive the bearing component to enter / exit from the centrifugal rotary drum; the sliding block is arranged on the bearing component in a sliding mode and can slide in the radial direction of the centrifugal rotating drum; the rotating roller is parallel to the central axis of the centrifugal rotating drum and is rotatably arranged on the sliding block; a main body of the telescopic part is fixedly installed on the bearing part, and a piston rod of the telescopic part is used for pushing the sliding block to face the inner wall face close to the centrifugal rotary drum so as to extrude the orange peel residues; by using the hesperidin residue separation device, the rotary roller can effectively extrude out the residual filtrate in the hesperidin residue, so that the waste amount of hesperidin is reduced, and the crystallization separation efficiency of hesperidin in the subsequent filtrate is improved.

The application provides a blood-activating and stasis-resolving external use plaster and a preparation process thereof, and the plaster is a microneedle-plaster composite preparation which is composed of a drug active ingredient, a sustained-release agent, a penetrating agent, an absorbent and a humectant. The drug active ingredient includes various Chinese herbal medicines such as Dysosma Versipellis, frankincense and myrrh, and the absorbent is a mixture of polyglutamic acid and snail polypeptide solution. The preparation process includes the steps of crushing medicinal materials, extracting with ethanol, concentrating and collecting cream, preparing a mixed solution, preparing a microneedle-plaster composite preparation and coating and the like. The snail polypeptide solution is obtained by enzymolysis of snail homogenate, and the humectant can be selected from hesperidin, glycerol and the like. The dissolvable material is selected from polylactic acid-hydroxyacetic acid copolymer and the like, and the ultrasonic power and frequency and the stirring speed of ultrasonic stirring all have certain ranges. The plaster has the effects of activating blood and resolving stasis and strengthening muscles and bones, and is suitable for external use. The preparation process is simple and feasible, and has high practical value and application prospect.

The present application provides an herbal supplement and method for alleviation of symptoms of gastroesophageal reflux disease (GERD). The herbal supplement comprises two or more herbal components selected from the group consisting of atractloydes extract, dandelion root extract, noni extract, hesperidin extract and quebracho extract.

To provide an inhibitor of expression of a receptor for adherence of pneumonia-causing bacteria.SOLUTION: An inhibitor of expression of a receptor for adherence of pneumonia-causing bacteria comprises at least one selected from the group consisting of hesperidin, cinnamaldehyde, citric acid hydrate, L-glutamic acid hydrochloride, and tartaric acid.SELECTED DRAWING: None

This invention belongs to the field of pharmaceutical technology and relates to a preparation process of a modified Huoxiang Zhengqi compound. The preparation process is as follows: Pinellia ternata and Angelica dahurica are percolated; Pogostemon cablin, Perilla frutescens, Citrus reticulata peel, Magnolia officinalis, and Atractylodes macrocephala are extracted in groups for 2-5 hours, and the total volatile oil is collected. The residues of each group are decocted with water, and the decoctions of each group are collected; Poria cocos, Platycodon grandiflorus, Glycyrrhiza uralensis, Areca catechu peel, Ziziphus jujuba, and Zingiber officinale are decocted with water to obtain a decoction; the total volatile oil is added to an inclusion material, and inclusion is obtained using an ultrasonic-assisted method; the percolation and decoctions are combined, concentrated, and centrifuged to obtain a supernatant; a preservative is added to the supernatant and the inclusion material to obtain the final product. This invention extracts medicinal materials containing volatile oils in groups and uses a cyclodextrin inclusion complexation process, which not only improves the total extraction rate of volatile oils, but also increases the content of hesperidin, hydrated oxypenicillin, magnolol, honokiol, patchouli flavonol, and baicalein, while improving the taste and flavor of the flavored patchouli flavonoid compound.

The invention relates to the technical field of gene engineering, and discloses an application of a CsCSE2 gene in increasing the content of citrus vicenin-2 and / or hesperidin, the CDS sequence of the CsCSE2 gene is shown as SEQ ID NO.1, and the protein sequence coded by the CsCSE2 gene is shown as SEQ ID NO.2. The invention also discloses a method for preparing the CsCSE2 gene for improving the content of citrus vicenin-2 and / or hesperidin. According to the invention, the CsCSE2 gene is found to be positively correlated with the content of citrus vicenin-2 and / or hesperidin for the first time, and in an experiment, the highest value of citrus of which the CsCSE2 gene overexpression vector is transformed can be 69.38% higher than that of citrus of which an empty vector is transformed; therefore, the CsCSE2 gene can be used as a candidate gene for breeding a new citrus variety with high-dimensioning-2 and / or hesperidin content, and has important significance in breeding a new functional citrus variety by adopting a genetic engineering method, accelerating the breeding process, reducing the breeding workload and improving the comprehensive utilization rate of citrus.

The present application provides an herbal supplement and a method for relieving symptoms of gastroesophageal reflux disease (GERD). The herbal supplement includes two or more herbal ingredients selected from the group consisting of atractylodes lancea extract, dandelion root extract, noni extract, hesperidin extract, and lignum extract.