129 results about "Hesperidin" patented technology

The invention discloses an application of a CsHCT1 gene in increasing the content of citrus flavonoids, belongs to the technical field of agricultural biological genetic engineering, and provides an application of the CsHCT1 gene in increasing the content of citrus flavonoids, and the nucleotide sequence of the CsHCT1 gene is as shown in SEQ ID NO.1. The invention further discloses an application of the CsHCT1 gene in increasing the content of citrus flavonoids. The invention also discloses a method for improving the flavone compound of the citrus peel by using the CsHCT1 gene. The method comprises the following steps: cloning the citrus CsHCT1 gene; a CsHCT1 overexpression vector is constructed; transforming the overexpression vector to agrobacterium tumefaciens to obtain an agrobacterium tumefaciens liquid; and injecting the CsHCT1 gene into the citrus fruit to obtain the CsHCT1 gene overexpressed citrus fruit. A new choice is provided for increasing the flavonoid compounds in the citrus peel, the content of vicenin-2 and / or hesperidin in the citrus peel is effectively increased, and the application has great application value for cultivating new functional citrus varieties and accelerating the breeding process.

The invention belongs to the field of pharmaceutical analysis, and discloses a method for simultaneously determining the contents of tolperidone hydrochloride, methyl hesperidin and vitamin B6 in Naoluotong capsules, the method comprises three steps of preparation of a mixed reference solution, preparation of a test solution and content determination, and the chromatographic conditions of the content determination are as follows: a C18 chromatographic column, a C18 chromatographic column, a C18 chromatographic column, a C18 chromatographic column, a C18 chromatographic column, a C18 chromatographic column, a C18 chromatographic column, a C18 chromatographic column and a C18 chromatographic column; a mobile phase is methanol (A)-0.1% glacial acetic acid solution (B), gradient elution is carried out for 0-5 min, and 96% of B is obtained; 5-6 min, 96%-60% of B is selected; 6-20 min, the content of B is 60%-50%; the volume flow rate is 1.0 ml / min; the column temperature is 30 DEG C; the detection wavelength is 291 nm; the sample size is 1 microliter. According to the method, synchronous detection of three components (tolperidone hydrochloride, methyl hesperidin and vitamin B6) with remarkable physicochemical property difference can be realized, the chromatographic condition is simple, and the detection speed is high.

The invention discloses a method for evaluating the comprehensive quality of dried orange peel based on a near-infrared spectrometer and an analytic hierarchy process-entropy weight method, and belongs to the technical field of optical analysis, and the method comprises the following steps: preparing a dried orange peel sample, acquiring near-infrared spectrum data of the dried orange peel sample, and preprocessing; acquiring the year information of the production place of the dried orange peel sample, and measuring the hesperidin content, the nobiletin content, the tangeretin content, the anti-inflammatory activity, the delicate flavor, the sweet taste, the bitter taste, the overall preference degree and the comprehensive score value of the dried orange peel in the dried orange peel sample; establishing a pericarpium citri reticulatae producing area year discrimination model, a hesperidin quantitative model, a nobiletin quantitative model, a tangeretin quantitative model, an anti-inflammatory activity quantitative model, a delicate flavor quantitative model, a sweet taste quantitative model, a bitter taste quantitative model, an overall preference quantitative model and a comprehensive score quantitative model; and detecting a to-be-detected dried orange peel sample, and evaluating the dried orange peel quality. The method has the advantages of economy, simplicity, convenience, rapidness, greenness and the like, and provides a basis for comprehensively evaluating the quality of the pericarpium citri reticulatae.

The invention discloses a plant-sourced anti-allergy soothing composition and application thereof, and belongs to the field of cosmetic processing.The plant-sourced anti-allergy soothing composition is prepared from hydrolyzed corn starch, saururus chinensis extract, carrot root extract and water-soluble hesperidin. Active ingredients of plant sources are deeply studied, and the anti-allergy soothing composition has the advantages that the plant-sourced anti-allergy soothing composition is prepared from the hydrolyzed corn starch, the saururus chinensis extract, the carrot root extract and the water-soluble hesperidin; a series of plant extracts with a synergistic effect are screened out, the plant-source multi-dimensional composition for relieving itching and repairing red is successfully researched and developed, and through the synergistic effect of the hydrolyzed corn starch, the saururus chinensis extract, the carrot root extract and the water-soluble hesperidin, the composition has the effects of relieving and repairing skin in a multi-dimensional mode, relieving itching and repairing red. In addition, the composition can repair the skin barrier for a long time and improve the tolerance of the skin, and meanwhile, the composition has good safety and stability and is suitable for various skin types, especially sensitive skin.

The invention discloses a composition for reducing blood pressure, blood sugar and blood fat, and belongs to the technical field of food processing. The moringa oleifera health-care food is prepared from moringa oleifera leaf powder, mulberry leaf powder, wall-broken ganoderma lucidum spore powder, passion fruit powder, prune powder, sea buckthorn powder, roxburgh rose powder, acerola cherry powder, angelica keiskei powder, corn stigma extract, perfume lemon powder, cyclocarya paliurus extract, radix puerariae extract, globe artichoke extract, lecithin, enzymes, OMEGA-3, arabinose and fructo-oligosaccharide. The plant composition which is sour with sweetness and good in taste is prepared by compounding and seasoning all edible raw materials such as glucosyl stevioside / hesperidin, the plant composition has good effects of reducing blood pressure, blood sugar and blood fat, and the adopted raw materials are healthy, non-toxic, easy to obtain, non-toxic to human bodies, especially livers, suitable for popular use and good in application prospect.

The invention provides a method for detecting medicinal components of a traditional Chinese medicine composition UPLC-MS / MS. The detection method can be used for identifying 14 medicinal components of the traditional Chinese medicine composition, namely saikoside A, saikoside D, naringin, paeoniflorin, hesperidin, chicoric acid, chlorogenic acid, salvianolic acid B, narcissoside, kaempferitrin, lobetyolin, amygdalin, hyperoside and rutin. And a reference is provided for evaluating the internal quality of the traditional Chinese medicine composition.

The invention provides a donkey-hide gelatin solid beverage and a preparation method thereof, the preparation method comprises the following steps: adding 8 times by mass of water into rhizoma polygonati, Chinese wolfberry, astragalus membranaceus, codonopsis pilosula, red dates, angelica sinensis and orange peel, soaking for 0.5 h, extracting for 2 h for the first time, adding 6 times by mass of water for extracting for 1.5 h for the second time, combining filtrates, and concentrating until the relative density is 1.23 g / cm < 3 > to obtain a water extraction concentrated solution; mixing the water extraction concentrated solution with maltodextrin, and drying to obtain an extract; and adding auxiliary materials into the extract and the donkey-hide gelatin, and molding to obtain the donkey-hide gelatin solid beverage. In the extraction step, proper soaking time, water adding amount and extraction time are combined, so that the ferulic acid content, the total polysaccharide content, the hesperidin content and the calycosin-7-glucoside content in the concentrated solution are all higher. And on the basis, subsequent drying and forming are carried out, so that the final donkey-hide gelatin solid beverage also has relatively high content of the components, and the content in each batch of products is stable.

A patchouli Qi-rectifying tablet, and a preparation method therefor and a qualitative and quantitative determination method therefor. The patchouli Qi-rectifying tablet comprises the following components in parts by mass: 0.5-1.5 parts of patchouli oil; 0.1-1 part of perilla leaf oil; 150-250 parts of common aucklandia root; 100-150 parts of rhizome of swordlike atractylodes; 10-100 parts of root of Ural licorice; 150-250 parts of Indian bread; 100-150 parts of dried tangerine peel; 100-150 parts of prepared tuber of pinellia; 100-150 parts of officinal magnolia bark; and 100-150 parts of fresh ginger. The qualitative and quantitative determination method for multiple components in the patchouli Qi-rectifying tablet comprises: performing qualitative determination of ten components, i.e., liquiritin, hesperidin, honokiol, costunolide, dehydrocostus lactone, magnolol, atractylodin, perilla ketone, nobiletin, and tangeretin, in the patchouli Qi-rectifying tablet; and performing quantitative determination of seven components, i.e., liquiritin, hesperidin, honokiol, costunolide, dehydrocostus lactone, magnolol, and atractylodin, in the patchouli Qi-rectifying tablet. According to the method, the operation is simple, the detection time is short, the linear relationship is good, the repeatability is good, the precision is high, the accuracy is high, and the stability is good.

This invention discloses a quality control method for an anti-snoring oral liquid and its application, belonging to the field of traditional Chinese medicine quality testing technology. The method includes thin-layer chromatography identification of Gleditsia sinensis thorns, Scrophularia ningpoensis, and Glycyrrhiza uralensis, as well as high-performance liquid chromatography (HPLC) determination of hesperidin content. By optimizing pretreatment, developing solvent, and colorimetric conditions, this invention achieves efficient and specific identification and quantification of multiple components in the compound preparation. The method is highly specific, produces clear spots, has good separation, and is tolerant to a wide temperature and humidity range (2-40℃, 20%-75%RH), requiring no strict environmental control, significantly improving detection efficiency and providing a reliable basis for establishing quality standards for this preparation.

The application provides a kind of zhangtou red pericarpium citri reticulatae UPLC quantitative fingerprint detection method, belongs to the quality detection technical field of traditional Chinese medicine.The detection method is prepared by reference solution, preparation of test solution, using ultra high performance liquid chromatography analysis, multiple batches of liquid chromatogram is introduced into "traditional Chinese medicine chromatographic fingerprint similarity evaluation system", 21 common peaks are identified, similarity matching is carried out with 21 common peaks, and the control spectrum of zhangtou red pericarpium citri reticulatae is generated.On this basis, combined with hesperidin, sweet orange flavone, chuan chenpisi, tangeretin, 5-dimethyl chuan chenpisi 5 flavonoid content determination, zhangtou red pericarpium citri reticulatae quantitative fingerprint is established.The application realizes that fingerprint detection and the content determination of 5 flavonoids of zhangtou red pericarpium citri reticulatae can be completed at the same time through one analysis, can quickly, comprehensively and accurately evaluate the quality of zhangtou red pericarpium citri reticulatae, has good reproducibility and stability, and can provide technical support for the uniformity and stability of zhangtou red pericarpium citri reticulatae quality.

The invention discloses a method for identifying Xinhui pericarpium citri reticulatae and pericarpium citri reticulatae, which is characterized in that four active ingredients including hesperidin, nobiletin, tangeretin and 2-methylamino methyl benzoate are simultaneously detected through high performance liquid chromatography, and distinguishing is realized according to detection data. The method comprises the following specific steps: preparing a mixed reference solution and a test solution, extracting a test with methanol, performing ultrasonic treatment, and filtering, specific chromatographic conditions are adopted for detection, the detection wavelength of the hesperidin is 283nm, and the detection wavelengths of the other three components are 330nm. Test results show that the contents of the four active ingredients in the Xinhui pericarpium citri reticulatae are high, the content of methyl 2-methylaminobenzoate is not lower than 0.020%, the ratio of the total amount of nobiletin and tangeretin to hesperidin is not lower than 0.17, and related indexes of the pericarpium citri reticulatae are remarkably low. The method is efficient, accurate, stable and reliable, and provides technical support for standardizing market circulation, guaranteeing product quality and promoting standardized development of the Xinhui pericarpium citri reticulatae industry.

The present invention discloses a method for constructing a fingerprint of Gualou Niu Bang decoction, relating to the technical field of analytical chemistry. The method is simple and has high precision, stability, and repeatability. Determination revealed that seven components, including geniposide, liquiritin, hesperidin, arctiin, baicalin, melaleucalyptol A-7-O-β-D-glucuronide, and wogonin, can be used as common characteristic peaks in the fingerprint of Gualou Niu Bang decoction for identification research.

The embodiment of the invention discloses a preparation method and a quality control method of fingered citron formula granules. The preparation method of the fingered citron formula granules comprises the following steps: crushing fingered citron decoction pieces, carrying out alcohol extraction twice, filtering, combining filtrates, carrying out vacuum concentration until no alcohol smell exists, and adding water to obtain a concentrated solution; enabling the concentrated solution to flow through a macroporous adsorption resin column for adsorption and alcohol elution, and collecting eluent; and concentrating the eluent under reduced pressure to obtain an extract, adding auxiliary materials, uniformly mixing, granulating, drying, and finishing to obtain the fingered citron formula granules. The preparation method provided by the invention can enrich hesperidin and other active ingredients, the prepared fingered citron formula granules are high in active ingredient content, and the preparation method is simple, high in operability and suitable for expanded production. The quality control method provided by the invention is accurate, reliable and good in repeatability, and can comprehensively evaluate the quality of the fingered citron formula granules.

The application discloses a suspension for improving DON-caused piglet intestinal toxicity and a preparation method and application thereof, and belongs to the technical field of feed additives, and comprises the following raw materials: hesperidin 40-60 mg / mL, sodium butyrate 50-150 mg / mL and PR-39 antibacterial peptide 10-15 mg / mL. The suspension provided by the application can also regulate intestinal flora balance, promote the proliferation of beneficial bacteria such as lactobacillus, and inhibit the growth of harmful bacteria. The effect of the suspension provided by the application is significantly better than that of single component or partial combination, and the suspension provides an efficient and safe nutritional intervention strategy for preventing and treating animal body damage caused by feed mycotoxin pollution.

The utility model provides a foreign matter detecting and removing device used before hesperidin finished product packaging, and belongs to the technical field of hesperidin processing. Comprising a conveying channel, materials are conveyed in the conveying channel through negative pressure suction, a foreign matter detector and a filtering assembly are arranged on the conveying channel, the filtering assembly comprises a filtering channel, a movable cylinder slides in the filtering channel, the movable cylinder comprises a partition plate and filtering cylinders coaxially and rotatably arranged on the two sides of the partition plate, and filtering pieces are arranged on the filtering cylinders; and a collecting assembly for collecting impurities filtered out by the filtering piece is arranged on the filtering channel. Impurities in hesperidin powder passing through the conveying channel can be filtered out through the filtering piece, when the foreign matter detector detects that the impurities pass through, the movable barrel is controlled to move in the filtering channel in a delayed mode, the other filtering barrel is moved into the conveying channel alternately, the filtering barrel with the impurities dumps and collects the impurities through rotation, and therefore the impurities can be filtered out through the filtering piece. The hesperidin powder filtering device has the effect of filtering hesperidin powder under the condition that equipment is not stopped.

The utility model relates to the technical field of extraction equipment, in particular to a hesperidin extraction device which comprises a support, a stand column, a heat preservation barrel and a reaction barrel. A stand column is connected to the middle of the upper surface of the support, a heat preservation barrel is connected to one side of the stand column, and a reaction barrel is connected to the inner wall of the heat preservation barrel. According to the hesperidin extraction device, water in the water tank is fed into the steam generator to generate steam by starting the water pump, the steam is fed into the heat preservation barrel to heat materials in the reaction barrel, then the steam is fed into the water tank from the heat dissipation pipe to preheat the water in the water tank, and then the hesperidin extraction device is heated; materials are fed into the filtering barrel by opening the discharging valve, the driving motor is started to filter the materials through the filtering barrel, the materials are stored in the liquid collecting barrel, separated liquid is taken out by opening the valve, then the liquid collecting barrel and the heat preservation barrel are staggered, the remaining materials are poured out through the filtering barrel, and then the hesperidin extraction device is convenient for solid-liquid separation.

The invention discloses a microbial fertilizer for treating citrus huanglongbing and a preparation method of the microbial fertilizer, and belongs to the field of agricultural biological control. 33 to 50 percent of sodium alginate-chitosan microspheres; 5 to 8% of hesperidin; 3-5% of nano zinc oxide; 2-4% of humic acid chelated boron; 2-4% of sucrose fatty acid ester; 2-4% of diatomite; wherein the preparation method of the sodium alginate-chitosan microspheres comprises the following steps: mixing sodium alginate with a microbial suspension, dropwise adding the mixture into a calcium chloride solution by using an injector, and standing and curing to form a microsphere core; and immersing the cured microspheres into a chitosan solution with the pH value of 6.0, taking out, and freeze-drying to obtain finished microspheres. Wherein the microbial suspension comprises trichoderma, bacillus and actinomycetes. The invention solves the problems of poor prevention and treatment effect of the existing Candidatus Liberobacter asiaticum, difficulty in colonization of the strain at phloem, short survival time and lack of targeted slow release function.

The utility model provides an operation table for hesperidin raw material pretreatment, and belongs to the technical field of hesperidin production. Comprising an operation table body, a cleaning tank is arranged on the operation table body, a liquid discharging pipe is arranged at the bottom of the cleaning tank, a valve is arranged on the liquid discharging pipe, a mounting frame is vertically and slidably arranged in the cleaning tank, a cleaning barrel used for containing hesperidin raw materials is rotatably arranged in the mounting frame, a feeding opening is formed in the outer wall of the cleaning barrel, and a cover plate is rotatably arranged at the feeding opening; a plurality of water inlet holes are formed in the outer wall of the cover plate and the outer wall of the cleaning barrel, a connecting piece is arranged between the cover plate and the cleaning barrel, a spreading and airing frame used for moving to the position below the cleaning barrel to receive hesperidin raw materials is horizontally and linearly arranged on the operation table body in a sliding mode, and a first driving piece used for driving the mounting frame to move is arranged on the operation table body. The second driving part is used for driving the airing rack to move, and a third driving part used for driving the cleaning barrel to rotate is arranged on the mounting frame. And the effect of reducing the labor intensity of workers is achieved.

The invention discloses a pet food for improving skeletal muscle system functions of elderly cats and a preparation method of the pet food. The pet food comprises the following raw materials in parts by mass: 40-45 parts of fresh chicken, 20-30 parts of fresh duck meat, 10-20 parts of starch, 1-5 parts of alfalfa particles, 1-5 parts of vitamins, 1-3 parts of mineral elements, 0.1-5 parts of probiotics, 0.01-0.2 part of an antioxidant, 1-5 parts of hydrolyzed chicken powder, 1-5 parts of a chicken extract, 1-5 parts of euphausia superba oil, 0.1-0.3 part of hesperidin and 0.1-0.5 part of a eucommia ulmoides extract. Wherein the functional components comprise euphausia superba oil, hesperidin and eucommia ulmoides plant extract, through scientific compatibility of all the components, the bone trabecula structure of cats can be improved through the synergistic effect, the bone density is enhanced, bone mineralization is promoted, cat body fat deposition is reduced, the muscle quality is improved, and therefore the bone muscle system of the cats is comprehensively improved.

The invention relates to a method for preparing trilobatin by taking hesperidin as a raw material, which comprises the following steps of: 1, hydrolyzing the hesperidin with the content of more than 90 percent as the raw material to remove rhamnose to obtain an intermediate 1; step 2, reacting the obtained intermediate 1 with p-hydroxybenzaldehyde to obtain an intermediate 2; 3, the obtained intermediate 2 is subjected to a reduction reaction and then purified, and the product trilobatin is obtained. The hesperidin is used as the main raw material to prepare the trilobatin, the hesperidin serving as the raw material is sufficient in source and low in cost and meets market requirements and has competitive advantages, and the preparation method is easy to operate and suitable for industrial production.

Nutritional supplements and compositions for activating the GLP-1 peptide and / or other hormones in a subject in order to regulate blood sugar, weight, and hunger of the subject are described in this application. The compositions include a first nutritional supplement containing specific amounts of hydrolyzed Acacia gum, lemon bioflavonoids, chromium, Berberis aristate, and hesperidin. The compositions also include a second nutritional supplement containing specific amounts of flax seed oil, resistant potato starch, resistant tapioca fiber, kombucha, Bacillus coagulans, Bacillus clausii, and allulose. A third composition includes the first and second nutritional supplements containing these ingredients in sufficient amounts to regulate the GLP-1 peptide and / or other hormones. These compositions are able to increase the GLP-1 concentration in the blood by up to about 140% without binding directly to any GLP-1 receptors. Other embodiments are also described.

The utility model discloses an extraction tank for preparing methyl hesperidin. The extraction tank comprises an extraction tank, a fixed seat is arranged at the top of the extraction tank, a motor and a stirring shaft are arranged on the fixed seat, and one end of the stirring shaft penetrates through a bearing on the fixed seat and is connected with the motor; a paddle wheel and a stirring frame are arranged on the stirring shaft, the stirring frame is provided with lantern rings, transverse rods, vertical rods and stirring blades, the three lantern rings are sequentially arranged on the stirring shaft at intervals, each lantern ring is provided with two transverse rods which are symmetrically arranged, the two vertical rods are connected with the transverse rods on the two sides of the lantern ring respectively, and the stirring blades are fixed to the vertical rods. The paddle wheel at the tail end of the stirring shaft can push liquid to the bottom of the tank, the stirring blades facing the outer side on the vertical rods can stir the liquid outwards, the stirring blades facing the inner side on the vertical rods can stir the liquid outwards, the front end openings of the stirring blades are large, the rear end openings of the stirring blades are small, and the stirring effect is achieved through the flow velocity difference of the liquid at the two end openings. The liquid is stirred in multiple directions, so that the stirring efficiency is improved.

The invention discloses a preparation method of a hesperidin regulated polyamide nanofiltration membrane. The preparation method comprises the following steps: S1, contacting the surface of a base membrane with a water-phase solution, standing, and removing the water-phase solution; s2, the surface of the base membrane with the water phase solution removed in the step S1 makes contact with an organic phase solution, interfacial polymerization is carried out, and the hesperidin-regulated polyamide nanofiltration membrane is prepared; the aqueous phase solution contains hesperidin, an amine monomer and water; the mass volume ratio of the hesperidin to the water is (5-10): 1g / L, and the mass volume ratio of the amine monomer to the water is (4-6): 1g / L; the organic phase solution contains an acyl chloride monomer and an organic solvent; the mass volume ratio of the acyl chloride monomer to the organic solvent is (1.3-2): 1g / L. The polyamide nanofiltration membrane has excellent inorganic salt / antibiotic selectivity while keeping excellent water flux and antibiotic rejection rate, and can be applied to devices such as sewage treatment equipment, seawater desalination equipment, industrial wastewater treatment and recycling equipment and the like.

The invention provides a Naoluotong capsule and a preparation method thereof, and belongs to the technical field of medicines. The Naoluotong capsule is prepared from the following components in parts by weight: 40 to 60 parts of radix salviae miltiorrhizae extract, 40 to 60 parts of rhizoma chuanxiong extract, 40 to 60 parts of radix astragali seu hedysari extract, 40 to 60 parts of tolperidone hydrochloride, 5 to 15 parts of methyl hesperidin, 1 to 4 parts of vitamin B6, 12 to 25 parts of polyvinylpyrrolidone K30, 13 to 25 parts of polyethylene glycol 400, 32 to 42 parts of corn starch, 3 to 7 parts of magnesium stearate and 200 to 250 parts of ethanol aqueous solution with the volume concentration of 70 to 80 percent. The Naoluotong capsule prepared by the invention is short in disintegration time, good in stability and high in tolperidone hydrochloride content.

The invention discloses a method for efficiently preparing hesperidin, which comprises the following steps: mixing grapefruit outer skin powder with petroleum ether, and carrying out ultrasonic-assisted extraction, suction filtration, concentration and drying to obtain a crude extract; dissolving the crude extract in petroleum ether, loading a sample to an SNAP KP-Sil normal-phase silica gel column, carrying out forward rapid liquid chromatography separation by taking petroleum ether-ethyl acetate as a mobile phase, identifying separated hesperelactone components through thin-layer chromatography, and merging the same components; carrying out reversed-phase preparative high-pressure liquid chromatography purification on the purified hesperidin component by virtue of a reversed-phase preparative high-pressure liquid chromatography column, and identifying the purified hesperidin component by virtue of thin-layer chromatography; carrying out structure identification on the collected components by using nuclear magnetic resonance and mass spectrometry, and determining the purity of the components by using high performance liquid chromatography; therefore, high-purity hesperidin can be rapidly prepared in a low-energy-consumption and solvent-saving manner.

The present application provides an herbal supplement and method for alleviation of symptoms of gastroesophageal reflux disease (GERD). The herbal supplement comprises two or more herbal components selected from the group consisting of atractloydes extract, dandelion root extract, noni extract, hesperidin extract and quebracho extract.

The present invention belongs to the technical field of feed additives, and specifically relates to a coated tannic acid preparation for weaned piglets, and a preparation method and use thereof. In view of the existing problems that weaned piglets are prone to high diarrhea rate, low feed intake, reduced immune function, and poor growth, the present invention provides a coated tannic acid preparation for weaned piglets. The coated tannic acid preparation for weaned piglets described in the present invention comprises: by weight percentage, 70-80% of the main material and 20-30% of the auxiliary material; the main material is a mixture of tannic acid, chlorogenic acid and hesperidin; the auxiliary material is a mixture of corn starch, β-cyclodextrin, sodium alginate, palm oil, acrylic resin and hydroxypropyl methylcellulose acetate succinate. The present invention also provides a preparation method and use of the above-mentioned preparation. The preparation of the present invention can improve the palatability of feed, reduce the diarrhea rate of weaned piglets, repair damaged intestines of weaned piglets, maintain the balance of intestinal microbial cells, and improve the disease resistance of weaned piglets.

The invention relates to the technical field of citrus processing, in particular to a composite green extraction technology for low-ester pectin, hesperidin and synephrine in young citrus fruits, which comprises the following steps: (1) crushing the dried young citrus fruits, putting the young fruit powder into an ultrasonic extraction tank, adding distilled water according to the material-liquid ratio of 1: 5-1: 30 g / mL, performing ultrasonic extraction for 20-60 minutes at the ultrasonic frequency of 20-80 kHz, and filtering to obtain filtrate; the temperature is 50-90 DEG C, centrifuging after the treatment is completed, taking supernate, and using residual filter residues for extracting hesperidin and synephrine. According to the technical scheme provided by the invention, composite extraction of low-ester pectin, hesperidin and synephrine in the young citrus fruits can be realized, and the extraction efficiency of functional active substances in the young citrus fruits is effectively improved; and the used extraction solvent has small pollution, is more environment-friendly compared with dichloromethane, ethyl acetate, methanol, acetone and other organic solvents used in the prior art, realizes reutilization of the extraction solvent, reduces environmental pollution, and reduces the extraction cost.

The invention discloses a preparation method and application of an enzyme preparation for enzymatically hydrolyzing bitter substances in citrus juice. Using Aspergillus tabingensis UA13 isolated in the early stage of the laboratory and mutagenized by atmospheric and room temperature plasma (ARTP) as the starting strain, limonin in lemon peel powder is used as an inducer to induce enzyme production, and the fermentation enzyme production process of the strain and the substrate affinity of the enzyme are studied. The results show that: in a 1 L fermentor medium, under the optimal conditions of limonin addition amount of 4.0 mg / L, inoculation amount of 10% (v / v), initial pH value of 5.0, stirring speed of 250 rpm, and fermentation at 30 °C, the highest limonin debittering enzyme activity can reach 48.52 U / mL. The results of the study on the substrate affinity of the enzyme for limonin show that the Km value of the limonin debittering enzyme for limonin is 0.622 mmol / L, indicating strong affinity. At the same time, the enzyme can still efficiently hydrolyze other bitter substances such as naringin and hesperidin, has good heat resistance and acid resistance, and has very good application effects in the citrus juice processing industry.

The invention discloses a preparation method of natural product co-assembled nanoparticles, and hesperidin and emodin are subjected to a co-assembly process to form carrier-free nanoparticles, so that the natural product co-assembled nanoparticles are obtained. The invention further provides the natural product co-assembled nanoparticles prepared by the preparation method and application of the natural product co-assembled nanoparticles. According to the natural product co-assembled nanoparticles provided by the invention, emodin which is rapidly released in an early stage can rapidly kill methicillin-resistant staphylococcus aureus (MRSA) on the surface of a wound, and a local antibacterial barrier is established; the hesperidin continuously released in the later stage plays an anti-inflammatory role by regulating and controlling the expression of inflammatory factors, and promotes the proliferation of fibroblasts and the secretion of angiogenesis-related growth factors (such as VEGF) at the same time. The'space-time synergistic release strategy 'provides a multifunctional platform integrating antibiosis, anti-inflammation and tissue repair for chronic wound treatment, and is suitable for accelerating healing of chronic wounds such as infection, diabetes and burn.