23 results about "Cyclocarya" patented technology

The invention belongs to the technical field of plant active ingredient extraction, and relates to a segmented enzymolysis-cascade membrane separation extraction method of cyclocarya paliurus combined-state selenium polysaccharide and the combined-state selenium polysaccharide prepared by the method. The method comprises the following steps: firstly, constructing a first extraction system for a cyclocarya paliurus leaf raw material, carrying out first-stage enzymolysis, inactivation and solid-liquid separation, separating a first liquid phase through a nanofiltration membrane to form a low-molecular selenium-containing component flow, and excluding the low-molecular selenium-containing component flow in a subsequent alcohol precipitation and recovery step; and constructing a second extraction system from the first solid phase, carrying out second-stage enzymolysis, inactivation and solid-liquid separation, sequentially carrying out microfiltration, first ultrafiltration, second ultrafiltration and nanofiltration washing on the second liquid phase, and carrying out ethanol precipitation to obtain the combined-state selenium polysaccharide. The proportion of target molecular weight interval components in the obtained product is increased, the mass fraction of polysaccharide is increased, and the proportion of inorganic selenium, the mass fraction of protein and the ash content are reduced.

This invention discloses a strain of *Carya volvacea* ZZNS-Y2 and its applications, belonging to the field of microbial application technology. The *Carya volvacea* (… Daldinia sp. The preservation number of ZZNS-Y2 is CCTCC NO: M 20241561. The method includes the following steps: *Cyclocarya paliurus* ZZNS-Y2 is cultured on one of the following solid-state fermentation media: corn, potato, corn grits, or rice. After extraction and concentration, the compounds crocusatin C and jasminodiol are separated by silica gel column chromatography, gel column chromatography, and high-performance liquid chromatography. The method of this invention is efficient, pollution-free, has mild reaction conditions, is not limited by plant resources, and is easy to scale up for production.

This invention relates to a rooting agent and seedling cultivation method for promoting rooting of *Cyclocarya paliurus* cuttings, belonging to the field of plant asexual reproduction technology. It solves one of the problems in existing *Cyclocarya paliurus* seedling cultivation: low rooting rate, long rooting time, few and poor-quality adventitious roots, and low transplant survival rate. This invention discloses a rooting agent for promoting rooting of *Cyclocarya paliurus* cuttings, which consists of two components, A and B. Component A consists of potassium indolebutyrate, soluble starch, chlorogenic acid, salicylic acid, and talc; component B consists of indolebutyric acid, sucrose, chlorogenic acid, vitamin C, and talc. Using the above-mentioned rooting agent and the corresponding seedling cultivation method, rapid rooting of *Cyclocarya paliurus* cuttings is achieved, with a rooting rate as high as 93.06%, and a large number and good quality of adventitious roots, resulting in a transplant survival rate of 90.37%.

The invention relates to the technical field of tea drinks, in particular to cyclocarya paliurus yellow tea and a preparation method thereof, and particularly relates to a cyclocarya paliurus leaf fermentation method and cyclocarya paliurus yellow tea formed by blending and mixing fermented cyclocarya paliurus leaves and black tea according to a certain proportion. The cyclocarya paliurus leaf fermentation method comprises the steps of withering, rolling, fermenting and drying. The water content of the withered cyclocarya paliurus leaves is 58-62 wt%; the cell destruction rate of the rolled cyclocarya paliurus leaves reaches 85% or above, and the strip forming rate of the leaves is 90% or above; the fermentation degree is 15-20%, 90% or more of the leaf color of the cyclocarya paliurus leaves after fermentation becomes copper red, and the liquor color is bright yellow; the drying comprises gross fire drying, spreading for cooling and complete fire drying which are performed in sequence; the moisture content after the first fire drying is 20wt%, and the moisture content after the full fire drying is 6wt%. The cyclocarya paliurus leaves are processed through a fermentation technology, the fermented cyclocarya paliurus leaves and the black tea are blended and mixed according to a certain proportion, the cyclocarya paliurus yellow tea which is unique in flavor and suitable for human health is formed, the cyclocarya paliurus yellow tea and the black tea are in synergistic interaction, the requirements of consumers for health functions are met, the acceptability of the public for the flavor of tea drinks is met, and the cyclocarya paliurus yellow tea is suitable for popularization and application. A new way is opened up for high-value utilization of the cyclocarya paliurus leaves.

This invention relates to the field of insect sex pheromone preparation technology, specifically to a method for preparing and applying a sex pheromone and its lure for controlling the Chinese moth *Cyclocarya paliurus*. Using GC-EAD and GC-MS technologies, this invention identified two sex pheromones, Z9-16:Ald and Z9,Z12,Z15-18:Ald, from the gonad extracts of female *Cyclocarya paliurus* moths. The sex pheromone provided by this invention has a clearly defined composition and optimized ratio. The lure preparation process is simple, has a long-lasting effect, and the trap has strong color adaptability. It enables efficient monitoring and green control of *Cyclocarya paliurus*, completely replacing chemical pesticides, is environmentally friendly, and suitable for widespread application in urban gardens and residential areas.

Flavonoid extract of Cyclocarya paliurus leaves is used in the preparation of antibacterial drugs and / or antibacterial agents against the bacteria that are resistant to conventional antibiotics. Experiments have proved that the flavonoid extract of C. paliurus leaves has obvious killing effect on methicillin-resistant Staphylococcus aureus strains, which provides a potential resolution to the problem that methicillin-resistant S. aureus strains are resistant to most beta-lactam antibiotics.

The invention discloses cyclocarya paliurus and tartary buckwheat granular tea with functions of reducing blood sugar, reducing blood lipid and resisting oxidation and a preparation method, and belongs to the technical field of functional food. The granular tea is prepared from 20-40% of cyclocarya paliurus powder, 60-80% of cured tartary buckwheat powder and a proper amount of food-grade auxiliary materials, wherein the cyclocarya paliurus powder comprises directly sieved powder and extracted concentrated powder. The preparation method comprises the following steps: pretreating the raw materials, mixing according to a scientific ratio, granulating by adopting a wet method or dry pressing to prepare 0.5-2.5 mm granules, drying at a low temperature until the moisture content is less than or equal to 5%, and sealing and packaging in a dark place. According to the present invention, the composition has characteristics of synergistic interaction, excellent taste and high active component absorption rate, is suitable for metabolic syndrome people including cold bodies, has characteristics of simple and controllable process, is suitable for industrial production, and has good application prospects.

The invention provides a construction method of a cyclocarya paliurus root system VIGS instantaneous silencing system. The construction method comprises the following steps: connecting a cyclocarya paliurus CpMYC2 gene with pTRV2 to obtain a recombinant vector pTRV2-CpMYC2; the method comprises the following steps: respectively transforming vectors pTRV2-CpMYC2 and pTRV1 into agrobacterium, so as to obtain agrobacterium containing a recombinant vector pTRV2-CpMYC2 and agrobacterium containing an auxiliary vector pTRV1; the method comprises the following steps: respectively inoculating a pTRV2-CpMYC2-containing agrobacterium tumefaciens and a pTRV1-containing agrobacterium tumefaciens into an LB liquid culture medium; collecting thalli, and resuspending the thalli by using the infection liquid; mixing the two resuspension solutions to obtain a dip dyeing bacterial solution; and infecting the root system of the cyclocarya paliurus with the infection bacterial liquid by adopting a vacuum pumping method or a root injury soaking method. The invention also provides application of the method in verification of cyclocarya paliurus gene functions. The cyclocarya paliurus root system is infected through the method, a target gene silencing system is constructed, and a foundation is laid for cyclocarya paliurus gene function research.

The invention provides specific primers and a rapid detection method used for detecting or identifying solanum tuberosum and application of the solanum tuberosum, the primer pair is PCTF1 / PCTR1, the nucleotide sequence of an upstream primer PCTF1 is shown as SEQ ID No: 1, and the nucleotide sequence of a downstream primer PCTR1 is shown as SEQ ID No: 2. The target sequence detected by the primer disclosed by the invention is a translation elongation factor (TEF) gene, the gene expression regulation is very conservative, and the gene sequence has high evolution conservative property and can be used as a DNA bar code of a point between species. The specific primer provided by the invention has the advantages of being good in specificity, high in sensitivity, economical, short in time consumption and the like when being used for detecting the brachycladium cucumerinum, and can play an important role in early diagnosis of the angelica sinensis root rot disease caused by Plectosphaerella cinerina.

This invention relates to the field of microbial technology, and provides a composite microbial suspension comprising a *Streptococcus pyogenes* R3 suspension and a *Cyclocarya pallida* N4 suspension in a volume ratio of 1-2:1-2; the *Streptococcus pyogenes* R3 classification name is... Herbaspirillum sp. R3 was deposited on September 20, 2023, at the China Center for Type Culture Collection (CCTCC), Wuhan University, Wuhan, China, with accession number CCTCC NO: M 20231757; the taxonomic name of Chlorella N4 is... Chryseobacterium endophyticum N4 was deposited on March 31, 2025, at the China Center for Type Culture Collection (CCTCC), Wuhan University, Wuhan, China, with accession number CCTCC NO: M 2025633. The aforementioned composite microbial suspension promotes nitrogen fixation and nitrogen conversion.

The invention provides a tea composition for reducing blood uric acid as well as a preparation method and application thereof, and belongs to the technical field of tea compositions. The tea comprises the following raw materials in parts by weight: 1-10 parts of Baihao silver needle, 1-9 parts of large-leaf white tea, 1-9 parts of mulberry leaf tea, 1-9 parts of cyclocarya paliurus, 1-5 parts of dandelion, 1-8 parts of pericarpium citri reticulatae, 1-6 parts of corn stigma and 1-5 parts of rose. The tea has the effect of reducing blood uric acid, and is rich in tea soup aroma, pure in taste and long in aftertaste. According to the composition, the Baihao silver needle is used as a monarch material to directly act on the pathogenesis of damp-heat; corn stigma and dandelion are used as ministerial drugs to construct a double mechanism of inhibiting generation and promoting excretion; cyclocarya paliurus, mulberry leaves and large-leaf white tea are used as assistants to regulate and control metabolic disorder in a multi-target manner; the dried orange peel and the rose are used as guide materials to regulate medicine properties and improve symptoms. The whole formula focuses on the characteristic of'deficiency in root and excess in superficies' of hyperuricemia, and gives consideration to etiological treatment and symptom relief.

When the four-wheel driving device of the cyperus esculentus harvester roller is used, power is distributed to the front side and the rear side through the middle gearbox to be output, the rear side is input to the rear gearbox through the rear transmission shaft, and the rear gearbox converts 90 degrees and then transmits the power to the rear left transmission shaft. The rear left transmission shaft transmits the power to a rear left driving wheel and a rear right driving wheel, the rear left driving wheel and the rear right driving wheel are connected through a rear right transmission shaft, and synchronous rotation of the two driving wheels on the rear side is achieved; the front side inputs power to the front gearbox through the front transmission shaft, the front gearbox converts 90 degrees and then transmits the power to the front left transmission shaft, the front left transmission shaft transmits the power to the front left driving wheel and the front right driving wheel, the front left driving wheel and the front right driving wheel are connected through the front right transmission shaft, and synchronous rotation of the two driving wheels on the front side is achieved. The cyperus esculentus screening device can drive the circular roller to rotate in the left-right direction to screen cyperus esculentus, and is stable in structure, low in failure rate and easy to replace.

The invention relates to the technical field of cyclocarya paliurus processing, and discloses a cyclocarya paliurus composite solid beverage and a preparation method thereof, and a plant composition comprises the following raw material components: cyclocarya paliurus leaves, rhizoma polygonati, haws and Chinese wolfberry fruits in a mass ratio of 3: 1: (1-3): 1. According to the cyclocarya paliurus beverage, four medicinal and edible plants including cyclocarya paliurus leaves, rhizoma polygonati, haws and Chinese wolfberry fruits are extracted by adopting a water extraction method, the method meets the requirements of related food safety standards of new food raw materials, meanwhile, rhizoma polygonati, haws and Chinese wolfberry fruits are added into the cyclocarya paliurus beverage, the flavor of the cyclocarya paliurus beverage is optimized, and meanwhile, the nutrition of the components is matched; the cyclocarya paliurus composite solid beverage has the advantages that the cyclocarya paliurus composite solid beverage is prepared by mixing four medicinal and edible plant dry extract powder of cyclocarya paliurus leaves, rhizoma polygonati, haws and Chinese wolfberry fruits with sodium carbonate, citric acid and erythritol, and the prepared cyclocarya paliurus composite solid beverage can be quickly dissolved without the conditions that the particles are difficult to dissolve, agglomerate, stick to cup walls and the like when being brewed with warm water.

The invention belongs to the technical field of biology, and particularly relates to a scrota-derived antibacterial peptide and application thereof. The antibacterial peptide from the scrota is SPC-1 or SPC-2, and the antibacterial peptide from the scrota is SPC-2; wherein the amino acid sequence of the SPC-1 is as shown in a sequence table SEQ ID NO. 1, and the amino acid sequence of the SPC-2 is as shown in a sequence table SEQ ID NO. 2. The scrota antibacterial peptide provided by the invention is novel in amino acid sequence, has remarkable antibacterial activity on gram-negative bacteria and gram-positive bacteria, shows broad-spectrum antibacterial property, and has the advantages of low hemolysis toxicity, low cytotoxicity and high safety, and by virtue of the characteristics, the scrota antibacterial peptide shows excellent antibacterial value; and a new resource is provided for the development of antibacterial products against the human pathogenic bacteria.

The invention provides a construction method of a cyclocarya paliurus leaf VIGS instantaneous silencing system. The construction method comprises the following steps: connecting a CpPDS gene with pTRV2 to obtain a vector pTRV2-CpPDS; the method comprises the following steps: respectively transforming carriers pTRV2-CpPDS and pTRV1 into agrobacterium tumefaciens, and then coating an LB solid culture medium; the method comprises the following steps: respectively inoculating agrobacterium containing a carrier pTRV2-CpPDS and agrobacterium containing a carrier pTRV1 into an LB liquid culture medium; collecting thalli, and resuspending the precipitate by using an infection solution; mixing the two resuspension solutions to obtain a dip dyeing bacterial solution; the leaf backs and leaf stems of the cyclocarya paliurus are scratched, and the cyclocarya paliurus are put into the dip-dyeing bacterial liquid to be soaked, or the scratched parts are wrapped with cotton absorbing the dip-dyeing bacterial liquid. The invention also provides application of the method in verification of cyclocarya paliurus gene functions. Cyclocarya paliurus leaves are infected through the method, a silence system of a target gene is constructed, the expression level of the target gene can be reduced, and a foundation is laid for cyclocarya paliurus gene function research.

This invention discloses a method for cultivating sea urchins that can improve their immunity. The method involves pre-introducing *Cyclocarya paliurus*, *Bacillus subtilis*, and photosynthetic bacteria (*Rhodopseudomonas palustris*) into the cultivation pond to cultivate a symbiotic system of bacteria and algae. During the sea urchin cultivation process, this symbiotic system is maintained by controlling the light intensity and applying specific nutrients. When combined with conventional sea urchin cultivation methods under this system, the immunity of sea urchins can be significantly improved, ultimately achieving the goal of increasing the survival rate and growth rate of the cultivated sea urchins.

The application discloses a method for preparing quercetin-3-O-glucuronide from Cyclocarya paliurus, and belongs to the technical field of Chinese herbal medicine development. The quercetin-3-O-glucuronide is separated from the Cyclocarya paliurus by utilizing the polar characteristics of the quercetin-3-O-glucuronide, and the purity reaches 98%, which indicates that the method for preparing the quercetin-3-O-glucuronide from the Cyclocarya paliurus has a development prospect.

The invention relates to the technical field of functional food, in particular to a composition with the effects of reducing blood sugar and regulating blood fat and application of the composition. The invention provides a composition with the effects of reducing blood sugar and regulating blood fat, the composition comprises a cyclocarya paliurus triterpenoic acid compound and a selenoprotein additive, and the effect is optimal when the mass ratio of ursolic acid to oleanolic acid to betulinic acid to arjiurolic acid in the cyclocarya paliurus triterpenoic acid compound is optimized to be 1: 3.21: 3.37: 0.74 through a response surface experiment. According to the invention, the cyclocarya paliurus triterpene component and the selenium food nutrition enhancer are used at the same time, so that the cyclocarya paliurus selenium food nutrition enhancer can reduce blood sugar, regulate blood fat and increase the antioxidant capacity of the body, also has the functions of inhibiting postprandial blood sugar rise, relieving insulin resistance and protecting liver islet, and can meet the selenium intake of 50-200 micrograms per day of adults recommended by Chinese Nutrition Association; the health-care food can be used as a novel functional food to be applied to prevention and health care of sub-health people with hyperglycemia and hyperlipidemia.

This disclosure relates to a method for controlling or preventing plant pathogenic fungi on plants or their plant propagation material, the plant pathogenic fungi belonging to the genus *Cyclocarya paliurus*, *Bacillus oryzae*, *Cyclocarya argentea*, *Cyclocarya gleditsiae*, *Cyclocarya rotundifolia*, *Bacillus aubergine*, *Botrytis cinerea*, *Botrytis tulipta*, *Cercospora multiflora*, *Cercospora kikuchi*, and / or *Cercospora davidii*, the method comprising applying a composition containing jassamycin to the plant, its plant propagation material, or the site thereof.

The invention belongs to the technical field of microalgae biology, and in particular relates to thaurita procumbens and a method for producing fucoxanthin by utilizing the thaurita procumbens. The invention relates to a phalaenopsis palustris which is named PCSM-1 and is preserved in the China Center for Type Culture Collection on January 11, 2024, and the preservation number is CCTCC NO: M 2024076. The content of fucoxanthin in the brachythecium cutescens is as high as 1.58 wt%, and the brachythecium cutescens can grow under both a photoautotrophic condition and a heterotrophic condition. The method comprises the following steps: firstly, performing heterotrophic fermentation on the palmaris cutescens so that the palmaris cutescens is rapidly proliferated and fermented; the method comprises the following steps: firstly, carrying out photoautotrophy and heterotrophy induction to quickly accumulate the fucoxanthin, so that the whole process is short in period and low in energy consumption, the yield of the fucoxanthin is far higher than that of the existing microalgae, and the problems of low production efficiency, high cost and the like of the current fucoxanthin can be solved.

The invention provides a hypoglycemic composition as well as a preparation method and application thereof, and belongs to the technical field of medicines. The feed additive is prepared from the following raw materials in parts by weight: 3-5 parts of selenium / chromium yeast embedding material, 2-3 parts of fresh ginger / discolor cinquefoil herb total flavonoids, 5-7 parts of charantin / mangiferin ethosome and 7-10 parts of cyclocarya paliurus extract. The blood sugar reducing composition prepared by the invention can improve blood sugar control and insulin resistance and stimulate intestinal L cells to secrete glucagon-like peptide-1 (GLP-1), so that insulin secretion is enhanced, the blood sugar level is reduced, glucagon secretion can be inhibited, generation of endogenous glucose is reduced, and the blood sugar reducing composition has a relatively good blood sugar regulating effect, and can be used for preparing a medicine for reducing blood sugar. The biocompatibility and the bioavailability are high, the patient compliance is good, and the application prospect is wide.

The invention discloses cyclocarya paliurus compound yoghourt with a blood glucose reducing function and a preparation method thereof, and belongs to the technical field of yoghourt preparation. Mixing the cyclocarya paliurus leaf powder, deionized water and enzyme-loaded particles, performing ultrasonic enzymolysis, performing centrifugal separation to obtain supernate, and sequentially inactivating and filtering the supernate to obtain cyclocarya paliurus leaf enzymatic hydrolysate; mixing the cyclocarya paliurus leaf enzymatic hydrolysate, defatted pure milk and cane sugar, homogenizing, and carrying out heat treatment to obtain a mixed base material; mixing and fermenting the mixed base material and the bacteria-loaded liposome compound, then cooling and refrigerating, and then filling to obtain the cyclocarya paliurus compound yoghourt. Through the preparation method disclosed by the invention, the finally prepared cyclocarya paliurus composite yoghourt has a good sensory state, a good viable count and a good hypoglycemic effect.

The invention provides a method for transient overexpression of exogenous genes on cyclocarya paliurus leaves, which comprises the following steps: carrying out double enzyme digestion on a target gene and an overexpression vector, and connecting the target gene and the overexpression vector by using ligase; transforming the recombinant overexpression vector into agrobacterium tumefaciens, and coating an LB solid culture medium with the agrobacterium tumefaciens; propagating the monoclone in an LB liquid culture medium; after the agrobacterium tumefaciens is collected, discarding the supernatant, and resuspending with the infection liquid to obtain an agrobacterium tumefaciens infection liquid; the method comprises the following steps: scratching leaf backs and leaf stems of cyclocarya paliurus, infecting leaves with an agrobacterium infection solution, or wrapping scratched parts with cotton absorbing the agrobacterium infection solution for infection. The invention further provides application of the method, and the exogenous gene is the CpSnRK1 alpha gene of the cyclocarya paliurus. The method has the advantages that a simple, convenient and efficient exogenous gene transient overexpression method for cyclocarya paliurus leaves is established for the first time, effectiveness of the method is verified through application of the CpSnRK1alpha gene, and potential possibility is provided for follow-up improvement of cyclocarya paliurus varieties through gene regulation and control.