29 results about "Leaf water" patented technology

The invention relates to the technical field of agricultural remote sensing and precise irrigation, in particular to a method and equipment for inverting leaf water potential of grapes before dawn based on multispectral remote sensing, and the method comprises the steps: measuring leaf water potential of representative grape plants in each planting test plot before dawn in different growth periods, and collecting canopy multispectral images in the noon period; preprocessing the remote sensing original image data; calculating a vegetation index; on the basis of the model fitting data set and the training feature set, constructing basic prediction models in different growth periods by adopting a unary linear regression algorithm, a multiple linear regression algorithm and a partial least square regression algorithm respectively; determining a PLSR inversion model according to the decision coefficient and the root-mean-square error; and carrying out pre-dawn leaf water potential prediction through the PLSR inversion model. Through the method, high-precision inversion of the water potential of the leaves before dawn based on the noon canopy multispectral data can be realized, and reliable technical support is provided for accurate management of vineyard moisture and intelligent irrigation.

The application discloses a drying method of hibiscus flowers. The method comprises the following steps: 1) enzyme inactivation and color protection: the hibiscus flowers are sequentially immersed in an acidic citric acid-vitamin C solution and a solution added with corn stem and leaf water extract, so as to efficiently inactivate oxidase and protect color; 2) composite film construction: a hibiscus leaf extract solution is used as a natural film-forming matrix, is compounded with hydroxypropyl methyl cellulose and the like to form a film-forming solution, the hibiscus flowers are immersed and coated, and a supercritical CO2 drying technology is used to form a uniform and porous protective film on the petal surface; and 3) hot air drying: two-stage gradient temperature and humidity control is adopted for final drying. The application can significantly inhibit enzyme browning and non-enzyme browning in the drying process, effectively reduce the loss of functional components such as anthocyanins and flavonoids, solve the problems of petal blackening, brittleness, fragility and the like caused by traditional drying, and the finished product is full in shape, bright in color, and green in the whole process.

The application discloses a kind of passion fruit plant health monitoring methods using spectral analysis, the method is by simultaneously collecting the spectral data of the top of plant new leaf, middle functional leaf and fruit, it is compared with the standard health spectrum model of prestorage, based on the diagnosis of magnesium deficiency of top leaf 530nm reflection peak, the diagnosis of nitrogen deficiency of middle leaf red edge position blue shift, the diagnosis of water deficiency of middle leaf water index rise, specific waveband spectrum distortion early warning disease and fruit spectrum form evaluation quality, realize multi-index comprehensive diagnosis;The application realizes multi-index diagnosis of plant nitrogen, magnesium nutritional status, water stress, potential disease and fruit quality by simultaneously collecting and analyzing the reflection characteristics of the top of passion fruit plant new leaf, middle functional leaf and fruit in specific spectral band, so as to overcome the problem of the prior art relying on single vegetation index or chlorophyll measurement, which leads to the problem of partial diagnosis information.

This invention provides a non-destructive detection method and device for plant leaf moisture based on dual-modal fusion, belonging to the field of plant water detection technology. The method includes: simultaneously acquiring the current dielectric, micro-deformation, and surface temperature parameters of the target plant leaf; after temperature compensation, using calibrated elastic coefficients and micro-deformation parameters to invert the dynamic thickness variation mapping relationship; accordingly, adaptive morphological interference elimination is performed on the dielectric parameters to obtain thickness-normalized dielectric parameters; finally, the above parameters are used as joint features input to a dual-modal fusion model, and the moisture detection results are decoupled and output. This invention decouples water loss and leaf morphological variation from the underlying physical mechanism, effectively eliminating interference and strain response hysteresis caused by changes in sensor electrode spacing, and significantly improving the accuracy and noise resistance of in-situ continuous monitoring of plant moisture across the entire range.

The invention relates to the field of plant biotechnology and molecular biology, in particular to application of a VvECT10 gene in regulation and control of drought resistance of grapes. A strain with overexpressed VvECT10 gene is slow in leaf water loss and light in withering degree under a drought condition, the content of malondialdehyde (MDA) and hydrogen peroxide (H2O2) is remarkably reduced, and the activity of peroxidase (POD) and catalase (CAT) is remarkably improved, which indicates that the overexpression of the VvECT10 gene can enhance the drought resistance of the grape plant and relieve oxidative damage.

The invention belongs to the technical field of plant planting, and provides a Hainan camellia oleifera high-survival-rate drought-resistant afforestation and biological nutrient solution preparation method. The special nutrient solution for the camellia oleifera comprises the following raw materials in parts by weight: 6-12 parts of a probiotic agent, 8-12 parts of potassium fulvate, 15-20 parts of potassium nitrate, 60-70 parts of a coco coir extract and 95-105 parts of water. Through the synergistic effect of the afforestation method and the special nutrient solution for camellia oleifera, the survival rate and preservation rate of camellia oleifera afforestation are remarkably increased. Wherein the special nutrient solution has high-temperature-resistant, drought-resistant and root-promoting effects, and can effectively promote the growth of roots of camellia oleifera, improve the water-retaining capacity of leaves and increase the content of available phosphorus in soil, thereby enhancing the stress resistance and growth vigor of plants; in the drought period, the leaf wilting degree of the oil-tea camellia adopting the drought-resistant afforestation method is far lower than that of a traditional method, the water content of soil is higher, and stage drought stress can be effectively resisted.

A kind of tea processing preparation process, it is related to tea processing technical field, solve the problem of different water content of tea fresh leaves due to different degree of sucking and biting of small green leafhopper adults and nymphs and different time interval from sucking and biting to picking.The preparation process includes fresh leaf pretreatment, collision green and aroma enhancement, steam + high hot air fixation, spreading, striping, drying, screening and picking, aroma enhancement and packaging.In the fresh leaf pretreatment process, small green leafhopper adults are put in, and small green leafhoppers are attracted to suck and bite fresh leaves by yellow light.In the collision green process, tea fresh leaves are made to jump by vibration to carry out collision green treatment.The present application solves the problem of different degree of fresh leaf water loss due to different degree of sucking and biting of small green leafhopper adults and nymphs and different time interval from sucking and biting to picking by putting small green leafhopper adults and nymphs to concentrate sucking and biting of tea fresh leaves.Meanwhile, the quality of cicada tea is effectively improved by temperature and humidity control, light supplement, collision green and aroma enhancement, steam + high hot air fixation.

The invention relates to the technical field of cosmetics, in particular to a soothing, repairing and moisturizing composition and application thereof, essence and a preparation method thereof. The soothing, repairing and moisturizing composition is prepared from the following components in parts by weight: 5 to 10 parts of myrtle leaf water, 1 to 5 parts of plantago major leaf extract, 0.5 to 1.5 parts of murdannia simplex leaf extract, 0.9 to 3 parts of lactic acid bacteria fermentation product and 0.1 to 2 parts of European larch wood extract. The effect is more comprehensive, more effective and more durable.

The present application provides a kind of regulation method for inducing phalaenopsis flower bud differentiation by day and night temperature difference, belong to plant culture technical field, the present application is by collecting each strain phalaenopsis genome single nucleotide polymorphism marker and historical phenotype data constructs temperature threshold prediction model, formulates differential day and night temperature control scheme and embeds optimal cooling curve planning module;During induction, use stem flow sensor and leaf microclimate probe to collect stem flow rate and leaf water potential in real time;Multi-channel physiological signal is input into day and night rhythm perception temperature control network, and the flower probability prediction value and target control temperature parameter are output in real time by artificial intelligence;With the flower rate and refrigeration energy consumption as double target, the energy-saving flowering game optimization model is constructed, the pareto frontier is solved on the agent surface, and the result is written back to the temperature control scheme;The technical problem that temperature control decision cannot simultaneously perceive plant rhythm physiological state and real-time water stress state, leading to the disconnection of temperature control strategy and actual physiological demand of plant is solved.

The application provides a gene mutant for reducing the leaf water loss rate and an application thereof, and belongs to the technical field of genetic engineering. The NtMYB184 gene mutant for reducing the leaf water loss rate has a mutated NtMYB184 gene sequence, as shown in SEQ ID NO. 2. The application also provides a gene editing vector, a transformant, a kit and a method for constructing the NtMYB184 gene mutant for reducing the leaf water loss rate, and an application of the method. The NtMYB184 gene mutant for reducing the leaf water loss rate provided by the application has a leaf water loss rate of 5.72% when placed for 300 minutes, which is lower than the water loss rate (6.87%) of a control WT, indicating that the leaf water loss rate of the NtMYB184 gene mutant (myb184) is lower than that of the control WT.

The application discloses a kind of coupling deep learning and physical mechanism's leaf water use efficiency estimation method, first, the environmental data and leaf water use efficiency of target area historical monitoring period are acquired;Then, construct the carbon dioxide concentration prediction model inside leaf, the trained carbon dioxide concentration prediction model inside leaf is used to predict carbon dioxide concentration inside leaf, the predicted value of carbon dioxide concentration inside leaf is substituted into formula to calculate leaf water use efficiency, the error between leaf water use efficiency estimation value and measured value is calculated, and the model is optimized by back propagation;Finally, the environmental data of target area monitoring period are acquired, and are input into the trained carbon dioxide concentration prediction model inside leaf, to obtain carbon dioxide concentration inside leaf;Leaf water use efficiency is calculated by carbon dioxide concentration formula inside leaf, and the estimation of leaf water use efficiency is completed.The method improves the estimation accuracy of leaf water use efficiency, and realizes continuous estimation.

The invention relates to the technical field of herbicides, in particular to a biological herbicide based on schizonepeta leaf water extract and a preparation method and application thereof. The invention discloses and proves that the schizonepeta leaf water extract has a remarkable and concentration-dependent allelopathy inhibition effect on seed germination and seedling growth of major weeds barnyard grass in farmland through systematic biological assay for the first time. The extract simultaneously and obviously inhibits the germination potential (germination speed), germination rate (germination ability), root length and bud length (early planting) of barnyard grass, realizes cooperative blocking of a plurality of key links in the early life stage of weeds, and has more thorough prevention and removal effects. The key concentration range (0.033 g / mL-0. 1 g / mL) and the optimal concentration (0.1 g / mL) of the schizonepeta leaf water extract for generating the remarkable inhibition effect are defined, and an accurate matching basis is provided for product development.

The invention relates to application of a potassium efficient gene DT1 in improvement of drought resistance of corn. It is found that the DT1 gene can significantly improve the drought resistance of corn, by overexpressing the DT1 gene in corn, a corn plant with higher drought resistance is successfully constructed, after drought treatment, the relative water content of leaves of the constructed DT1 overexpressed plant is higher than that of wild type leaves, the water loss rate of in-vitro leaves is reduced, and the drought resistance is significantly enhanced; and a gene resource is provided for cultivating a new variety. Compared with a traditional breeding mode, the drought-resistant breeding method has the advantages that the breeding time is short, the purposiveness is high, the breeding period is remarkably shortened, the breeding efficiency is improved, the drought-resistant breeding method has great application value in crop molecule improvement, and a theoretical basis is provided for clarification of a molecular mechanism of plant drought resistance.

The invention belongs to the field of plant genetic engineering, and relates to application and a method of a GmCRY2a gene or a GmCRY2a protein in regulating and controlling the water loss rate of soybean leaves. According to the invention, two GmCRY2a genes in soybean are knocked out, so that a phenotype with high water loss rate of leaves appears. According to the CRISPR / Cas9 knockout technology constructed by the invention, pure and gmcry2a1a2 mutant soybean plants can be obtained, and compared with control soybeans, the water loss rate of in-vitro leaves is remarkably increased along with the increase of time, so that the method can be applied to screening soybeans suitable for growing in high-temperature and arid regions all year round, and has a huge application prospect.

The invention discloses an aloe barbadensis leaf water composition for removing acnes and application thereof, and the composition is prepared from the following components in percentage by mass: 91 to 97 percent of aloe barbadensis leaf water, 1 to 4 percent of nicotinamide, 1 to 4 percent of arbutin, 0.1 to 0.4 percent of benzalkonium bromide and 0.1 to 0.6 percent of chlorhexidine acetate. The invention also provides a preparation method and application of the composition. The method comprises the following steps: weighing the raw materials in proportion, then mixing the aloe barbadensis leaf water, nicotinamide, arbutin, benzalkonium bromide and chlorhexidine acetate in a container, stirring and dissolving uniformly, sampling and detecting to be qualified, disinfecting and sterilizing, and finally, sealing, packaging and storing the aloe barbadensis leaf water, the nicotinamide, the arbutin, the benzalkonium bromide and the chlorhexidine acetate. The composition is used for preparing skin care products for removing acnes. The aloe barbadensis leaf water composition for removing acnes, prepared by the preparation method disclosed by the invention, adopts acnes-removing components derived from the nature, is safe in acnes removal without hurting the skin, restores the skin to a healthy state while repairing the damaged skin, and inhibits the re-breeding of acnes and bacteria.

Disclosed herein is a system and methods for leaf detection and extraction. The extracted leaf may be used for leaf water potential analysis. In some embodiments, the method comprises identifying the leaf from a point cloud based on an image, determining a pose of the leaf based on the point cloud, and cutting and storing the leaf based on the pose of the leaf.

The application discloses application of N-acetyl-L-cysteine in improving drought resistance of plants and belongs to the technical field of plant cultivation.The application first proposes application of N-acetyl-L-cysteine (NAC) in improving drought resistance of Brassica napus L.And through experiments, it is proved that compared with a control group, the fresh weight, dry weight, root length and chlorophyll content of the Brassica napus L seedlings that are sprayed with 150 muM of N-acetyl-L-cysteine on leaves are improved, the leaf water loss rate is reduced, and the drought stress tolerance of the Brassica napus L is enhanced.This reflects that the drought stress tolerance of the Brassica napus L treated with N-acetyl-L-cysteine is obviously improved.Further experiments are conducted to obtain an optimal application concentration of 150 muM, so that the N-acetyl-L-cysteine can be optimally used in improving the drought stress tolerance of the Brassica napus L.

The invention relates to the technical field related to plant genetic engineering, in particular to application of a gene BnaMLP328L in drought resistance of brassica napus L. The drought resistance and sensitive drought germplasm transcriptome analysis finds that the expression difference of the gene BnaMLP328L under drought stress is related to the drought resistance, functional verification shows that the drought resistance is weakened by overexpression of the gene, the drought resistance is enhanced by gene editing, and the gene BnaMLP328L can be applied to drought resistance of brassica napus L.. The mechanism is to adjust the pore opening of the plant. According to the invention, a CRISPR / Cas9 editing vector of the gene is constructed, and agrobacterium tumefaciens-mediated transformation is carried out on brassica napus, so that a strain with significantly improved drought resistance (showing that the water loss rate of in-vitro leaves is reduced, the relative water content is improved and the like) is obtained. The method fills the gap of brassica napus drought-resistant gene resources, the technical scheme is mature and feasible, key support is provided for drought-resistant germplasm creation and new variety breeding, the oilseed rape planting area is enlarged, and edible oil supply safety is guaranteed.

The present application relates to the field of agricultural informatization and crop water physiological monitoring technology, solves the technical problems of insufficient estimation accuracy, single response factor, lack of causal (physiological) explanation and engineering application closed loop in the existing leaf water use efficiency estimation method, especially relates to a leaf water use efficiency estimation method based on multi-source feature coupling and application, synchronously collects photosynthetic parameters, environmental factors and electrophysiological parameters to construct a feature vector when the leaf is fully expanded, uses an integrated learning model to predict the photosynthetic parameters and calculate the leaf water use efficiency; SHAP is used to give feature contribution and identify the main control factor; a WUE thermal map is generated by combining coordinate interpolation for zoned irrigation and agronomic optimization, and the sliding time window is used for online updating to improve the timing robustness. Field verification of cotton, corn and wheat shows that the method has high precision, strong adaptability and is easy to deploy and popularize.

The invention provides an elastic-plastic analysis method and system for a plant leaf water physiological state, and relates to the technical field of plant physiological ecology and crop stress resistance research, and the method comprises the following steps: obtaining original data of a leaf dehydration process, and calculating an extraplastid water volume and a co-plastid water relative water content based on the original data; correcting the original data to determine the initial position of the co-mass water analysis; based on the corrected data, automatically dividing the swelling pressure drop stage into a linear elastic stage and a nonlinear elastic-plastic stage, and performing index-linear combination model fitting on the nonlinear elastic-plastic stage; performing derivation calculation on the index-linear combination model of the nonlinear elastic-plastic stage to generate an elastic-plastic change rate and an elastic adjustment capability index; the elastic-plastic change rate represents the elastic-plastic transition dynamic state of the cell wall; the elasticity adjusting capability index represents the elasticity adjusting capability of the plant cell wall. The precision and depth of plant moisture physiological research and stress resistance breeding evaluation can be improved.

The invention relates to the technical field of gene engineering, in particular to application of a GhUGP1 gene in regulation and control of drought resistance of cotton. Phylogenetic analysis reveals that the GhUGP1 gene has high conservative property in the plant evolution process, and promoter cis-acting element analysis shows that the GhUGP1 gene contains rich stress response elements and indicates that the GhUGP1 gene participates in abiotic stress response. By constructing a GhUGP1 overexpression plant, it is found that the GhUGP1 overexpression plant shows a more drought-resistant phenotype, a lower in-vitro leaf water loss rate and a faster rehydration recovery capability under drought stress. Physiological index detection shows that active oxygen accumulation of overexpressed plants is remarkably reduced, antioxidant enzyme activity is improved, malondialdehyde content is reduced, callose deposition is reduced, and pore opening is reduced. Results show that the GhUGP1 plays a key role in maintaining moisture balance, enhancing active oxygen scavenging ability and regulating stomatal movement by positively regulating the drought resistance of the cotton.

The utility model relates to the field of leaf water potential measurement, in particular to a portable leaf water potential measuring device based on an optical sensor, which is simple in structure and capable of measuring leaf water potential data in a short time. The measuring device comprises a light source module, an optical sensor module, a signal processing sensor module, a buffer pad, a rotating shaft, a display module, a function key and a battery, the device is connected with the upper leaf chamber and the lower leaf chamber through the rotating shaft to clamp the leaf, and the buffer pad prevents the leaf from being damaged by excessive extrusion and prevents external natural light from entering the leaf chambers to affect measurement. The optical sensor module receives the light intensity of the LED lamp source which transmits the leaves or reflects the leaves and transmits the light intensity information to the signal processor module, and the signal processor module converts the light intensity change into a leaf water potential value based on a pre-established relation model of the leaf water potential and the light intensity change. Measured data are displayed in real time through the display module, and the battery supplies power to the whole device. The device is suitable for leaf water potential measurement.

The invention discloses an intelligent leaf water potential monitoring system and method based on a multi-element flexible thin film, and belongs to the technical field of leaf water potential monitoring, the specific structure comprises a plurality of flexible thin film sensing units, a wireless transmission unit and a remote monitoring terminal, the flexible thin film sensing units are respectively installed on the surfaces of a plurality of leaves, and the wireless transmission unit is connected with the remote monitoring terminal; the plurality of flexible thin film sensing units are connected to the remote monitoring terminal through the wireless transmission unit, the flexible thin film sensing units are used for collecting the temperature, humidity, illuminance and tension value of the leaf, each flexible thin film sensing unit comprises a flexible thin film and a plurality of sensing units uniformly distributed on the flexible thin film, and the plurality of sensing units are attached to the surface of the leaf. According to the method, the leaf water potential of the plant leaf moisture condition can be monitored in real time, losslessly and accurately, and a scientific basis is provided for agricultural irrigation decision making.

The invention discloses a cultivation method of drought-resistant brassica napus, and belongs to the technical field of biology. The method comprises the following steps: by taking brassica napus as a research object, extracting leaf RNA (Ribonucleic Acid), performing reverse transcription to obtain cDNA (Complementary Deoxyribonucleic Acid), cloning a BnaNCED3 gene, constructing an overexpression and silence vector, performing transformation verification by escherichia coli, and introducing agrobacterium; infecting the oilseed rape hypocotyl explant through agrobacterium tumefaciens, and carrying out co-culture, differentiation screening and rooting culture to obtain a transgenic plant; and finally, screening strains with high expression of BnaNCED3, and identifying the drought tolerance. By improving the expression level of the BnaNCED3 gene, the drought survival rate of the oilseed rape is remarkably improved, the water loss rate of leaves is reduced, and an efficient technical means is provided for cultivation of drought-resistant oilseed rape varieties.

The present application belongs to the field of agricultural technology, and in particular to a dynamic induction system for water requirement of cranberry and a method thereof. The existing traditional irrigation method is often based on a fixed schedule or simple soil moisture measurement, which cannot accurately reflect the actual water requirement of cranberry in time, easily leading to insufficient irrigation or over-irrigation, wasting water resources and affecting the growth and yield of cranberry. The present application includes a monitoring module: stem flow rate, leaf water potential, soil water content at different depths and positions, and air temperature and humidity, light intensity and wind speed data of cranberry; a data acquisition module: collecting stem flow rate and leaf water potential of cranberry. The present application can realize accurate monitoring, real-time evaluation and scientific prediction of the water requirement of cranberry, and further guide reasonable irrigation decision and environmental regulation, improve irrigation efficiency and water resource utilization efficiency.

The invention provides application of a vaccinium bracteatum leaf extract in preparation of a product with an effect of bidirectionally regulating oral flora, and belongs to the technical field of oral care. The vaccinium bracteatum leaf water extract is obtained through a mild and green preparation method, and it is found that the vaccinium bracteatum leaf water extract can inhibit growth of oral pathogenic bacteria at a specific concentration and promote proliferation of oral beneficial bacteria at the same time, so that oral micro-ecological balance is effectively adjusted. The vaccinium bracteatum leaf extract can also be compounded with quercetin to prepare a composite nano-emulsion, and the vaccinium bracteatum leaf extract has the characteristics of being natural, safe and efficient, provides a new raw material choice for developing a new generation of health products for regulating oral flora, and has a wide application prospect.

The invention belongs to the technical field of plant molecular breeding, and particularly relates to an application of a BnIAA19-related biological product in regulating and controlling the wax content and / or drought resistance of oilseed rape epidermis, and the BnIAA19 refers to any one of the following: 1) a nucleotide sequence as shown in SEQ ID NO.1; 2) a nucleotide sequence having at least 90% of identity with the SEQ ID NO.1; and 3) a nucleotide sequence capable of being hybridized with SEQ ID NO.1. The invention discloses a key negative regulation effect of BnIAA19 in regulation of epidermis wax synthesis and drought resistance, and the leaf epidermis wax content can be remarkably improved by knocking out the gene, so that the leaf water loss rate is effectively reduced, the water retention capability and survival rate of plants under drought stress are improved, and the photosynthetic water utilization efficiency is synergistically improved; the discovery provides a clear new target for stress-resistant breeding of oilseed rape and cruciferae crops, accurate regulation and control can be realized by utilizing mature technologies such as CRISPR / Cas9 and the like, and the method has the advantages of strong operability and wide application prospect.

The application discloses a digital plant canopy water evaporation amount data mining system, comprising a main control unit, which is connected with a detection unit for detecting and acquiring water evaporation data of the plant canopy position, the detection unit comprising: a leaf physiological detection module for detecting leaves of the plant canopy position and acquiring the influence of environmental factors on leaf physiological processes; the leaf physiological detection module comprises a leaf temperature detection module; through the design of the application, on the basis of retaining existing conventional water evaporation detection means, corresponding analysis and detection can be made according to leaves of different plants, so that the acquired leaf water evaporation data is more accurate, and further detection of the conditions influencing leaf water evaporation is realized, so that the accuracy of data is further improved, the existing detection mode is more comprehensive, and data security is provided for later analysis.