32 results about "Abscisic acid" patented technology

The invention discloses a sugarcane ScPP2C49 gene and application thereof, and relates to the technical field of plant biology, the nucleotide sequence of the ScPP2C49 gene is as shown in SEQ ID No.1, and the amino acid sequence of protein coded by the ScPP2C49 gene is as shown in SEQ ID No.2. The gene is induced to express by drought and abscisic acid, and the encoded protein of the gene is positioned in a cell nucleus. Functional studies show that after overexpression of the ScPP2C49 gene in the plant, the drought resistance of the plant is negatively regulated through various mechanisms of inhibiting an abscisic acid signal channel, hindering stomatal closure under drought stress, weakening active oxygen scavenging ability, reducing photosynthetic efficiency, inhibiting root growth and the like. The invention also provides a recombinant overexpression vector containing the gene and a method for obtaining a transgenic plant with reduced drought resistance by using the vector through an agrobacterium-mediated method. According to the invention, a new gene resource is provided for deep analysis of a plant drought-resistant molecular mechanism, and an important target gene is provided for cultivation of high-stress-resistance crop varieties through a reverse genetics means (such as gene knockout).

The invention relates to the technical field of plant genetic engineering, in particular to a peanut bacterial wilt resistance related gene Ah6Q1KS5, an encoding protein thereof, a biological material and application of the gene Ah6Q1KS5 in disease-resistant breeding. Through whole genome identification and functional analysis, the gene Ah6Q1KS5 which is remarkably related to bacterial wilt resistance in a peanut SAMS gene family is screened, and the gene is induced by ralstonia solanacearum, salicylic acid (SA), abscisic acid (ABA) and methyl jasmonate (MeJA) to be remarkably expressed in a peanut bacterial wilt resistant variety H108. Peanut and tobacco plants over-expressing Ah6Q1KS5 can significantly reduce cell death, inhibit growth of ralstonia solanacearum, and enhance resistance to bacterial wilt. The molecular marker can be used for analyzing the molecular mechanism of peanut bacterial wilt resistance, provides new gene resources and technical means for cultivating bacterial wilt resistant peanut varieties, and has important application value in the field of peanut breeding.

The FaCP5L1 and FaCP5L3 genes and application thereof in regulating fruit ripening and postharvest quality belong to the field of agricultural biological genetic engineering technology. It is found for the first time that the cruciferin coding genes FaCP5L1 and FaCP5L3 can directly participate in the regulation of strawberry ripening and postharvest quality, including but not limited to the regulation of abscisic acid (ABA) content, ripening process, soluble solid content, hardness, color, anthocyanin content. The expression of FaCP5L1 and FaCP5L3 can promote the ripening of strawberry fruit and the formation of postharvest quality; the expression reduction can slow down the ripening of strawberry fruit and the formation of postharvest quality. Accordingly, the ripening of strawberry fruit and the postharvest quality traits can be regulated by reasonably utilizing the genes, and the ripening and postharvest quality related breeding can be guided. The present application provides a theoretical basis and technical support for regulating the ripening of strawberry fruit and the postharvest quality traits by using genetic engineering technology, and has important application value.

The invention discloses a pesticide composition containing trinexapac-ethyl and S-abscisic acid and a preparation method of the pesticide composition. 4.0%-6.0% of trinexapac-ethyl and 0.1%-0.5% of S-abscisic acid are used as main effective components, and a surfactant and liquid caustic soda are used as auxiliary components to prepare an aqueous solution; and further performing micro-fluidization nano-emulsification to prepare nano-emulsion particles with D90 of 50-150nm, embedding the nano-emulsion particles in a sodium alginate or chitosan matrix, and applying the nano-emulsion particles to foliar spraying or seed coating / root coating dual-mode administration. According to the technology, quick spraying effectiveness and early seed coating seedling protection are both considered, an obvious synergistic effect is achieved according to the proportion of 5: 5, and 90%-98% of the lodging prevention effect can be achieved within the effective component concentration range of 20-50 g / L. Residues can be degraded within 1-3 days, and the advantages of high efficiency, long acting and environmental protection are achieved.

The invention relates to a composition and a medicament for inhibiting sprouting of wheat ears and application of the composition and the medicament, and belongs to the technical field of plant growth regulators. Experiments prove that when prohexadione calcium or ethephon is combined with S-abscisic acid for use, the effect of the composition is obviously superior to that of single use of S-abscisic acid, and the drug effect lasts for a longer time. It is proved for the first time that a synergistic effect exists between prohexadione calcium or ethephon and S-abscisic acid, the drug effect can be better achieved, and meanwhile the use amount of S-abscisic acid is remarkably reduced. The composition for inhibiting wheat pre-harvest germination provided by the invention effectively overcomes the defects of unstable effect, pesticide residue and the like in the prior art, can improve the effect of singly using S-abscisic acid, and achieves the purposes of controlling pre-harvest germination and not influencing wheat yield.

The application discloses a functional gene PaSPL8 for regulating apricot fruit ripening, and the nucleotide sequence of the PaSPL8 gene is shown as SEQ ID NO. 1. The functional gene PaSPL8 for regulating apricot fruit ripening is used for regulating apricot or tomato fruit ripening, and the fruit ripening is delayed by increasing the expression amount of the PaSPL8 gene; or the functional gene PaSPL8 for regulating apricot fruit ripening is used for regulating the content of abscisic acid in plant tissues. The application verifies that the PaSPL8 gene regulates the ripening of apricot fruits by regulating the content of ABA in apricots, and therefore, the PaSPL8 gene can be used for apricot breeding.

The invention belongs to the technical field of agricultural compositions, and particularly relates to an agricultural composition for promoting disease resistance and quality improvement of fruit trees. The active ingredients of the agricultural composition are geraniol and S-abscisic acid, a field test shows that the mass ratio of geraniol to S-abscisic acid is (8-16): (15-7), the geraniol and S-abscisic acid have a synergistic effect, and the geraniol and S-abscisic acid can remarkably improve the single fruit weight and soluble solids of facility cherries and peaches, improve the anthocyanin content in the facility cherries and improve the vitamin C content in the peaches, so that the yield of the facility cherries and peaches is increased. Meanwhile, the morbidity of the gummosis of the fruit trees is remarkably reduced, and the disease resistance of the tree bodies is enhanced. The invention provides more solutions for solving the problems of frequent gummosis and fruit quality decline in fruit tree cultivation.

The application discloses a Phyllostachys edulis-derived PeDHN2 gene and application of a coded protein thereof, and belongs to the technical field of plant biological breeding. A nucleotide sequence of the PeDHN2 gene is shown as SEQ ID NO. 11; and a sequence of the coded protein of the PeDHN2 gene is shown as SEQ ID NO. 12. The PeDHN2 gene is cloned from the Phyllostachys edulis, and experiments show that the gene is significantly up-regulated under salt stress, drought and abscisic acid (ABA) stress, and after being introduced into rice through a transgenic technology, the germination rate and root growth ability of the rice under salt stress are significantly improved. The application provides a key gene resource and technical support for molecular design breeding of salt-tolerant crops.

The application discloses a gene PtoTAR2 for improving drought resistance and sensitivity to ABA of poplar and application thereof, a nucleotide sequence of the gene is shown in SEQ ID NO. 1, an amino acid sequence of a coded protein is shown in SEQ ID NO. 3, the gene can negatively regulate drought resistance and sensitivity to ABA of the poplar, provides a candidate gene for cultivating new germplasm of the poplar with improved stress resistance and without affecting growth, and effectively shortens a breeding cycle. The application also discloses a method for improving drought resistance and sensitivity to ABA of the poplar and related application, and the drought resistance and the sensitivity to ABA of the poplar are specifically enhanced by silencing expression of the gene PtoTAR2 in the poplar, which provides an effective way for cultivating new germplasm of the poplar with improved stress resistance and without affecting growth, and has important significance for promoting sustainable development of forestry.

The invention discloses a northern tree seed breeding greenhouse with a monitoring function, and relates to the technical field of agricultural breeding facilities. The northern tree seed breeding greenhouse with the monitoring function comprises a main body, an irrigation unit and a nursery garden, a main body comprises a transparent greenhouse film, a framework and a monitoring assembly, the framework guarantees the structural stability, the monitoring assembly drives a monitoring camera with a built-in near infrared spectrum sensor through a slidable moving cross rod and a telescopic rod, and accurate monitoring of the ABA content of plant bud abscisic acid is achieved; the main body is also provided with an air inlet pipe with an electric heating wire and a main control module integrated with multiple sensors; the irrigation unit is linked with the main control module through an electromagnetic valve to realize precise irrigation; a floor heating module and various soil sensors are arranged in the nursery garden. According to the invention, cooperative monitoring and intelligent regulation and control of the environment in the greenhouse and the plant physiological indexes in the northern cold environment can be realized, and the breeding success rate and efficiency are improved.

The invention discloses a functional gene PaSPL8 for regulating and controlling maturity of apricot fruits. The nucleotide sequence of the gene PaSPL8 is as shown in SEQ ID NO. 1. The functional gene PaSPL8 for regulating and controlling apricot fruit ripening is used for regulating and controlling apricot or tomato fruit ripening, and fruit ripening is delayed by increasing the expression quantity of the gene PaSPL8; or the functional gene PaSPL8 for regulating and controlling the maturity of the apricot fruits is used for regulating and controlling the content of abscisic acid in plant tissues. The invention verifies that the PaSPL8 gene regulates and controls the maturity of apricot fruits by regulating and controlling the ABA content of apricot, so that the PaSPL8 gene can be used for apricot breeding.

The invention discloses a recombinant Yarrowia lipolytica engineering strain for producing abscisic acid and application of the recombinant Yarrowia lipolytica engineering strain, and belongs to the technical field of genetic engineering and bioengineering. According to the invention, heterologous synthesis of ABA is realized in the Yarrowia lipolytica, precursor supply is optimized by enhancing carbon flow in a metabolic pathway, the activity of key enzymes BcABA2 and BcABA3 is improved by adopting a semi-rational strategy, NADPH regeneration engineering is implemented to enhance the oxygen uptake ability of a strain, a cytoplasm-peroxisome engineering system is constructed, and the oxygen uptake ability of the strain is enhanced. The ABA synthesis is further enhanced by utilizing the characteristic of high-yield acetyl coenzyme A of peroxisome. According to the invention, fermentation conditions are further optimized, so that the ABA accumulation concentration of the constructed engineering strain in a 5 L bioreactor reaches 2554.36 mg / L, and the highest titer of ABA biosynthesis reported so far is created. The metabolic modification strategy provides important reference for biosynthesis of other terpenoids.

The invention discloses a functional gene PaACO2 for regulating and controlling maturity of apricot fruits and application of the functional gene PaACO2. The nucleotide sequence of the gene PaACO2 is shown as SEQ ID NO. 1. The PaACO2 gene is used for regulating and controlling the maturation of apricot fruits, and the maturation of the apricot fruits is promoted by increasing the expression quantity of the PaACO2 gene, or the maturation of the fruits is delayed by inhibiting the expression of the PaACO2 gene. The functional gene PaACO2 for regulating and controlling the maturation of the apricot fruits is used for promoting the maturation of the tomato fruits or regulating and controlling the content of abscisic acid and / or aminocyclopropane carboxylic acid in plant tissues. The invention verifies that the PaACO2 gene regulates and controls the maturation of apricot fruits by regulating and controlling the ABA and ACC contents of apricots, so that the PaACO2 gene can be used for breeding apricots to regulate and control the maturation of apricot fruits.

This invention belongs to the field of biotechnology, specifically relating to the cassava transcription factor MeWRKY30 and its applications. This invention cloned the transcription factor gene of MeWRKY30 from cassava. Overexpression of this gene in cassava can improve the resistance of transgenic cassava plants to low-temperature and drought stress. The effects of qRT-PCR on cassava... MeWRKY30 Gene expression was significantly upregulated after drought and treatment with the plant hormone abscisic acid (ABA). After recovery from low temperature treatment, the malondialdehyde content of the overexpressing cassava lines decreased, wilting was reduced, root growth inhibition was lessened, and the number of cortical cells in the root tip meristem was significantly increased.

The invention relates to the technical field of fruit tree cultivation, in particular to a method for thinning kiwi fruits and / or increasing the ascorbic acid content of the kiwi fruits and application. The invention provides an application and method of a plant growth regulator composition in thinning kiwi fruits and / or increasing the ascorbic acid content of kiwi fruits, and the plant growth regulator composition comprises broad beans, ethephon and 6-benayl aminopurine. According to the method disclosed by the invention, the single fruit rate and the ascorbic acid content of the kiwi fruits of Jinchu No.1 can be remarkably increased, and the double-fruit rate of the kiwi fruits is remarkably reduced, but the three-fruit rate and the total fruit setting rate of the kiwi fruits are not influenced.

ABSCISIC ACID SOLVATES AND LIQUID COMPOSITIONS CONTAINING ABSCISIC ACID. The present invention relates to novel modifications of abscisic acid, liquid compositions of abscisic acid, and their use in agrochemical applications. The modifications and compositions of the invention contain abscisic acid in the form of a solvate with at least one N-alkyl pyrrolidone, wherein the N-alkyl group has 3 to 6 carbon atoms. These solvates allow formulations with high concentrations of abscisic acid.

The application belongs to the technical field of biological genetic engineering, and particularly relates to application of a ZmDIR4 gene in regulation of drought sensitivity of corn. The application finds through experiments that after the ZmDIR4 gene is knocked out, the dry matter accumulation of mutant corn plants after drought stress, the activity of antioxidant enzymes in the antioxidant metabolic pathway, the accumulation of proline, chlorophyll and lignan, and the activation degree of the hormone metabolic pathway (zeatin and abscisic acid) are all significantly higher than those of wild type and overexpression materials, proving that the ZmDIR4 gene has a negative regulation function in drought stress resistance of corn. The ZmDIR4 gene can be used to create new corn germplasm with improved drought resistance, and provides a new idea for corn drought resistance.

This invention discloses a functional gene, PaNCED5, that regulates apricot fruit ripening. The nucleotide sequence of the PaNCED5 gene is shown in SEQ ID NO.1. This PaNCED5 gene can be used to regulate apricot fruit ripening by increasing its expression level to promote ripening or by inhibiting its expression to delay ripening. This functional gene, PaNCED5, can also be used to promote tomato fruit ripening or to regulate the content of abscisic acid (ABA) in plant tissues. This invention verifies that the PaNCED5 gene regulates apricot fruit ripening by controlling the ABA content in apricots; therefore, the PaNCED5 gene can be used in apricot breeding.

The invention belongs to the technical field of plant genetic engineering, and particularly relates to a wheat TaSAPK3 gene and application thereof in the aspect of regulating plant drought resistance. The wheat TaSAPK3 gene is subjected to abscisic acid (ABA), drought and salt stress induced expression, meanwhile, the wheat TaSAPK3 gene is overexpressed and transformed into wheat in an overexpression mode, and it is found that compared with a wild type, the drought resistance of a transgenic plant overexpressed with TaSAPK3 is remarkably improved; after the wheat TaSAPK3 gene in wheat is knocked out, the drought resistance is obviously reduced. It is proved that the wheat TaSAPK3 gene positively regulates the drought resistance of wheat, a new gene resource is provided for molecular breeding of wheat, and the wheat TaSAPK3 gene has great significance in genetic improvement of the drought resistance of wheat.

The invention discloses a functional gene PaNCED5 for regulating and controlling maturity of apricot fruits. The nucleotide sequence of the gene PaNCED5 is as shown in SEQ ID NO. 1. The PaNCED5 gene is used for regulating and controlling the maturation of apricot fruits, and the maturation of the apricot fruits is promoted by increasing the expression quantity of the PaNCED5 gene, or the maturation of the fruits is delayed by inhibiting the expression of the PaNCED5 gene. The functional gene PaNCED5 for regulating and controlling the maturation of the apricot fruits is used for promoting the maturation of tomato fruits or regulating and controlling the content of abscisic acid in plant tissues. The invention verifies that the PaNCED5 gene regulates and controls the maturity of apricot fruits by regulating and controlling the ABA content of apricot, so that the PaNCED5 gene can be used for apricot breeding.

The invention discloses a Siberia apricot kernel size regulation gene as well as an encoding protein, a primer pair and application thereof. The regulation gene is a PsPYL12 gene and a PsABI5 gene; the nucleotide sequence of the PsPYL12 gene is as shown in SEQ ID NO. 1, and the nucleotide sequence of the PsABI5 gene is as shown in SEQ ID NO. 2. The amino acid sequences of the encoding proteins of the two regulatory genes are respectively shown as SEQ ID NO.3 and SEQ ID NO.4; nucleotide sequences of a forward primer and a reverse primer for amplifying the PsPYL12 gene are respectively as shown in SEQ ID NO. 5 and SEQ ID NO. 6; and the nucleotide sequences of a forward primer and a reverse primer for amplifying the PsABI5 gene are respectively as shown in SEQ ID NO.7 and SEQ ID NO.8. The invention also discloses a kit for detecting the PsABI5 gene. The regulation gene participates in the abscisic acid signal channel to positively regulate the seed size of the plant. The invention provides a theoretical basis and a technical tool for high-yield breeding of the Siberia apricot.

The invention discloses a lodging-resistant regulator for highland barley and a method for improving lodging-resistant capability of the highland barley in a seedling stage. The lodging-resistant regulator comprises the following components: a plant growth retardant, a nutritional agent, a biological active agent, a plant extract and a surfactant, wherein the plant growth retardant is prepared from 5 to 10 parts by mass of uniconazole and 3 to 6 parts by mass of prohexadione calcium; the nutritional agent comprises 15-20 parts by mass of potassium silicate, 3-5 parts by mass of sodium selenate, 2-3 parts by mass of zinc sulfate, 2-3 parts by mass of manganese sulfate, 2-3 parts by mass of ammonium molybdate, 2-3 parts by mass of borax and 2-3 parts by mass of EDTA chelated magnesium. The invention relates to the technical field of plant growth regulators. According to the lodging-resistant regulator for the highland barley and the method for improving the lodging-resistant capability of the highland barley in the seedling stage, the regulator can more accurately act on the highland barley in the seedling stage through the synergistic effect of multiple components and the combination of a step-by-step using method.

This invention relates to a ginkgo glycosyltransferase GbUGT74E1 and its application in regulating plant height and drought stress tolerance. GbUGT74E1 The nucleotide sequence is shown in SEQ ID No. 1, and the amino acid sequence is shown in SEQ ID No. 2. Its protein is located in the cell nucleus and cytoplasm. This invention provides a method for regulating... GbUGT74E1 A method to regulate plant height and drought stress tolerance through gene expression. Experiments have shown that overexpression of gene expression in tobacco... GbUGT74E1 It can significantly reduce relative conductivity, malondialdehyde content, and hydrogen peroxide content under drought stress, while increasing antioxidant enzyme activity, free proline content, and the accumulation of various flavonoids. Furthermore, this gene, when overexpressed in tobacco and ginkgo, can regulate the content of resistance-related hormones such as abscisic acid, jasmonic acid, and salicylic acid, as well as auxins. This invention clarifies... GbUGT74E1 Its function in plant growth and drought tolerance provides a new approach to regulating plant height and drought resistance through genetic engineering.

The application provides a gene (GA2ox9) for regulating seed dormancy and germination of plants and application thereof. The GA2ox9 affects alpha amylase activity and soluble sugar content by regulating the content of active gibberellin (GA) in endosperm, changes abscisic acid (ABA) signal in the embryo, and further regulates seed dormancy and plant ear germination. The GA2ox9 gene deletion leads to the decrease of seed dormancy and the occurrence of ear germination, and the overexpression of the GA2ox9 gene improves seed dormancy. The application provides a new way for improving seed dormancy and reducing ear germination of plants.

The application provides a grape VvKCS11 The application provides a promoter and application thereof, and belongs to the technical field of plant genetic engineering. ProKCS11 The nucleotide sequence is shown as SEQ ID NO. 1; a recombinant vector, an expression cassette, a recombinant cell or a recombinant bacteria containing the nucleotide sequence shown as SEQ ID NO. 1. ProKCS11 The application further provides a promoter with the function of responding to salt stress, drought stress, osmotic stress, ion stress or abscisic acid. ProKCS11 The salt stress, drought stress and ion toxicity can induce high expression of a downstream gene driven by the promoter, and the expression has certain tissue specificity. ProKCS11 The application further provides a transgenic plant. VvKCS11 The application can improve the stress resistance of the transgenic plant.

The invention discloses a composition and a method for improving waterlogging tolerance of pear trees. The composition comprises a compound microbial fertilizer for root application and a growth regulator for leaf application, wherein the compound microbial fertilizer for root application contains bacillus amyloliquefaciens and trichoderma harzianum T22; the growth regulator for leaf application contains a uniconazole solution and a prohexadione calcium suspending agent. The composition has a synergistic effect on the overground part and the underground part of the pear tree, and by enhancing physiological response of leaves and root systems, the damaged root systems are effectively prevented from secondary soil-borne diseases, normal growth of tree bodies is recovered in time, the leaf falling rate and the fruit falling rate caused by waterlogging are reduced, and sharp reduction of the yield is avoided; the waterlogging tolerance of the pear trees is improved, and the death rate of pear tree bodies after waterlogging is effectively reduced.

The invention discloses a rice promoter induced by abscisic acid ABA and application of the rice promoter. The nucleotide sequence of the promoter is shown as SEQ ID NO. 1. The promoter provided by the invention belongs to an ABA-induced promoter, i.e., the promoter responds to an ABA signal to open the expression of a downstream target gene. When a plant suffers from adversity stress (endogenous ABA rising), the promoter can rapidly start efficient expression of a downstream gene. And under the normal growth condition, the low activity is maintained. The expression mode effectively avoids plant metabolism burden and growth inhibition caused by continuous overexpression of the stress-resistant gene. The characteristic of on-demand expression is suitable for cultivating high-yield and stable-yield stress-resistant new varieties in plant genetic engineering breeding.

The invention relates to a preparation method and application of a paclobutrazol-containing nano-pesticide, in particular to a paclobutrazol and prohexadione calcium nano-pesticide, which is prepared from the following raw materials in 1000 parts by weight: 100 to 400 parts by weight of paclobutrazol, 25 to 100 parts by weight of prohexadione calcium, 1 to 100 parts by weight of nano-carrier, 40 to 400 parts by weight of auxiliary agent and the balance of water, wherein the weight ratio of paclobutrazol to prohexadione calcium is 4: 1. The nano pesticide reduces the pesticide particle size, enhances the permeability and adsorbability of the pesticide, resists rain wash, improves the pesticide liquid absorption rate, solves the technical problems that the traditional pesticide blocks a spray head and the pesticide liquid is easy to drift especially for airplane spraying, can reduce the pesticide usage amount, has a good promotion effect on wheat yield increase, and has a good application prospect. And the wheat quality can be improved.

The invention discloses an ABA (abscisic acid) related protein CAR4 gene of a C2 structural domain of alfalfa and application of the ABA related protein CAR4 gene. The cDNA sequence MsCAR4 of the alfalfa C2 structural domain ABA related protein CAR4 gene is cloned from alfalfa, and research finds that after the gene is over-expressed in alfalfa, branches are reduced, flowering is advanced, and the yield is reduced; meanwhile, after the gene is interfered in medicago sativa, branches are increased, flowering is delayed, and the yield is improved. According to the invention, genes for regulating late flowering of medicago sativa and promoting branch growth are excavated and related functional studies are carried out, so that a theoretical foundation can be laid for variety improvement of medicago sativa and breeding of germplasm resources.