46 results about "Abscisic acid" patented technology

The invention discloses a sugarcane ScPP2C49 gene and application thereof, and relates to the technical field of plant biology, the nucleotide sequence of the ScPP2C49 gene is as shown in SEQ ID No.1, and the amino acid sequence of protein coded by the ScPP2C49 gene is as shown in SEQ ID No.2. The gene is induced to express by drought and abscisic acid, and the encoded protein of the gene is positioned in a cell nucleus. Functional studies show that after overexpression of the ScPP2C49 gene in the plant, the drought resistance of the plant is negatively regulated through various mechanisms of inhibiting an abscisic acid signal channel, hindering stomatal closure under drought stress, weakening active oxygen scavenging ability, reducing photosynthetic efficiency, inhibiting root growth and the like. The invention also provides a recombinant overexpression vector containing the gene and a method for obtaining a transgenic plant with reduced drought resistance by using the vector through an agrobacterium-mediated method. According to the invention, a new gene resource is provided for deep analysis of a plant drought-resistant molecular mechanism, and an important target gene is provided for cultivation of high-stress-resistance crop varieties through a reverse genetics means (such as gene knockout).

The invention discloses a gene for degrading chlorophyll as well as an encoding protein and application thereof, and belongs to the fields of molecular biology, plant genetic engineering and horticulture. Protein coded by the gene is located in chloroplast, and chlorophyll degradation can be efficiently promoted under various stress conditions. By performing different treatments on Huangkui tea trees and performing gene function verification in model plants such as nicotiana benthamiana and arabidopsis thaliana, it is found that the CsSGRs gene is subjected to induced expression by factors such as natural aging, abscisic acid, illumination intensity and low temperature, and the gene participates in a chlorophyll degradation approach, so that the functions are redundant. In addition, a bZIP family transcription factor CsABF4 is screened out, and CsSGRs gene expression can be regulated and controlled. The invention provides gene resources and theoretical basis for regulating and controlling the chlorophyll content of plants and improving related characters of plant leaf color, and has potential application value in the fields of plant breeding and gardening.

The invention relates to the technical field of plant genetic engineering, in particular to a peanut bacterial wilt resistance related gene Ah6Q1KS5, an encoding protein thereof, a biological material and application of the gene Ah6Q1KS5 in disease-resistant breeding. Through whole genome identification and functional analysis, the gene Ah6Q1KS5 which is remarkably related to bacterial wilt resistance in a peanut SAMS gene family is screened, and the gene is induced by ralstonia solanacearum, salicylic acid (SA), abscisic acid (ABA) and methyl jasmonate (MeJA) to be remarkably expressed in a peanut bacterial wilt resistant variety H108. Peanut and tobacco plants over-expressing Ah6Q1KS5 can significantly reduce cell death, inhibit growth of ralstonia solanacearum, and enhance resistance to bacterial wilt. The molecular marker can be used for analyzing the molecular mechanism of peanut bacterial wilt resistance, provides new gene resources and technical means for cultivating bacterial wilt resistant peanut varieties, and has important application value in the field of peanut breeding.

The FaCP5L1 and FaCP5L3 genes and application thereof in regulating fruit ripening and postharvest quality belong to the field of agricultural biological genetic engineering technology. It is found for the first time that the cruciferin coding genes FaCP5L1 and FaCP5L3 can directly participate in the regulation of strawberry ripening and postharvest quality, including but not limited to the regulation of abscisic acid (ABA) content, ripening process, soluble solid content, hardness, color, anthocyanin content. The expression of FaCP5L1 and FaCP5L3 can promote the ripening of strawberry fruit and the formation of postharvest quality; the expression reduction can slow down the ripening of strawberry fruit and the formation of postharvest quality. Accordingly, the ripening of strawberry fruit and the postharvest quality traits can be regulated by reasonably utilizing the genes, and the ripening and postharvest quality related breeding can be guided. The present application provides a theoretical basis and technical support for regulating the ripening of strawberry fruit and the postharvest quality traits by using genetic engineering technology, and has important application value.

The application provides cassava MeFER4 Gene and application thereof in improving sensitivity of plants to stress. By constructing overexpression vector containing MeFER4 Gene and transforming into host plant tissues such as Arabidopsis and cassava through infection method, plants sensitive to drought, abscisic acid, oxidation, salt and osmotic stress and other stress are obtained. Research shows that, MeFER4 It is a negative regulatory factor of plant stress response and plays a negative regulatory role in plant drought resistance and the like, and the transgenic plant is more sensitive to stress. The application is beneficial to elucidating the role of MeFER4 Gene in stress response from the molecular mechanism, and has important significance for directional genetic improvement of plant drought resistance and plant variety selection.

The invention discloses a pesticide composition containing trinexapac-ethyl and S-abscisic acid and a preparation method of the pesticide composition. 4.0%-6.0% of trinexapac-ethyl and 0.1%-0.5% of S-abscisic acid are used as main effective components, and a surfactant and liquid caustic soda are used as auxiliary components to prepare an aqueous solution; and further performing micro-fluidization nano-emulsification to prepare nano-emulsion particles with D90 of 50-150nm, embedding the nano-emulsion particles in a sodium alginate or chitosan matrix, and applying the nano-emulsion particles to foliar spraying or seed coating / root coating dual-mode administration. According to the technology, quick spraying effectiveness and early seed coating seedling protection are both considered, an obvious synergistic effect is achieved according to the proportion of 5: 5, and 90%-98% of the lodging prevention effect can be achieved within the effective component concentration range of 20-50 g / L. Residues can be degraded within 1-3 days, and the advantages of high efficiency, long acting and environmental protection are achieved.

The invention relates to a composition and a medicament for inhibiting sprouting of wheat ears and application of the composition and the medicament, and belongs to the technical field of plant growth regulators. Experiments prove that when prohexadione calcium or ethephon is combined with S-abscisic acid for use, the effect of the composition is obviously superior to that of single use of S-abscisic acid, and the drug effect lasts for a longer time. It is proved for the first time that a synergistic effect exists between prohexadione calcium or ethephon and S-abscisic acid, the drug effect can be better achieved, and meanwhile the use amount of S-abscisic acid is remarkably reduced. The composition for inhibiting wheat pre-harvest germination provided by the invention effectively overcomes the defects of unstable effect, pesticide residue and the like in the prior art, can improve the effect of singly using S-abscisic acid, and achieves the purposes of controlling pre-harvest germination and not influencing wheat yield.

The application discloses a functional gene PaSPL8 for regulating apricot fruit ripening, and the nucleotide sequence of the PaSPL8 gene is shown as SEQ ID NO. 1. The functional gene PaSPL8 for regulating apricot fruit ripening is used for regulating apricot or tomato fruit ripening, and the fruit ripening is delayed by increasing the expression amount of the PaSPL8 gene; or the functional gene PaSPL8 for regulating apricot fruit ripening is used for regulating the content of abscisic acid in plant tissues. The application verifies that the PaSPL8 gene regulates the ripening of apricot fruits by regulating the content of ABA in apricots, and therefore, the PaSPL8 gene can be used for apricot breeding.

The present invention relates to the technical field of fruit industry, and specifically discloses a regulation method and application for promoting and strengthening the flower formation of citrus. The regulation method is to spray flower-promoting regulator A during the ripening stage of autumn shoots of citrus; spray flower-promoting regulator B from 1 week before the appearance of flower buds in the following year to the flower bud stage of citrus. The components and spraying concentration of the flower-promoting regulator A are: S-abscisic acid 15-50 μg / ml, prohexadione-calcium 25-100 μg / ml, chlorophyllin iron 0.05-1 μg / ml, and the balance is an agriculturally acceptable adjuvant; the components and spraying concentration of the flower-promoting regulator B are: S-abscisic acid 1-35 μg / ml, 6-benzylaminopurine 10-70 μg / ml, and chlorophyllin iron 0.1-2 μg / ml, and the balance is an agriculturally acceptable adjuvant. The present invention can regulate the balance of flower-forming hormones in axillary buds of the tree body, effectively increase the flower formation amount of citrus in the following year, improve the quality of flower buds and the flowering uniformity, reduce the proportion of deformed flowers, reduce the difficulty of fruit retention, increase the citrus yield and improve the quality.

The invention belongs to the technical field of agricultural compositions, and particularly relates to an agricultural composition for promoting disease resistance and quality improvement of fruit trees. The active ingredients of the agricultural composition are geraniol and S-abscisic acid, a field test shows that the mass ratio of geraniol to S-abscisic acid is (8-16): (15-7), the geraniol and S-abscisic acid have a synergistic effect, and the geraniol and S-abscisic acid can remarkably improve the single fruit weight and soluble solids of facility cherries and peaches, improve the anthocyanin content in the facility cherries and improve the vitamin C content in the peaches, so that the yield of the facility cherries and peaches is increased. Meanwhile, the morbidity of the gummosis of the fruit trees is remarkably reduced, and the disease resistance of the tree bodies is enhanced. The invention provides more solutions for solving the problems of frequent gummosis and fruit quality decline in fruit tree cultivation.

The application discloses a Phyllostachys edulis-derived PeDHN2 gene and application of a coded protein thereof, and belongs to the technical field of plant biological breeding. A nucleotide sequence of the PeDHN2 gene is shown as SEQ ID NO. 11; and a sequence of the coded protein of the PeDHN2 gene is shown as SEQ ID NO. 12. The PeDHN2 gene is cloned from the Phyllostachys edulis, and experiments show that the gene is significantly up-regulated under salt stress, drought and abscisic acid (ABA) stress, and after being introduced into rice through a transgenic technology, the germination rate and root growth ability of the rice under salt stress are significantly improved. The application provides a key gene resource and technical support for molecular design breeding of salt-tolerant crops.

The application discloses a gene PtoTAR2 for improving drought resistance and sensitivity to ABA of poplar and application thereof, a nucleotide sequence of the gene is shown in SEQ ID NO. 1, an amino acid sequence of a coded protein is shown in SEQ ID NO. 3, the gene can negatively regulate drought resistance and sensitivity to ABA of the poplar, provides a candidate gene for cultivating new germplasm of the poplar with improved stress resistance and without affecting growth, and effectively shortens a breeding cycle. The application also discloses a method for improving drought resistance and sensitivity to ABA of the poplar and related application, and the drought resistance and the sensitivity to ABA of the poplar are specifically enhanced by silencing expression of the gene PtoTAR2 in the poplar, which provides an effective way for cultivating new germplasm of the poplar with improved stress resistance and without affecting growth, and has important significance for promoting sustainable development of forestry.

The invention belongs to the technical field of plant genetic engineering, and particularly relates to application of an LsSLR1 gene or a related biological material containing the LsSLR1 gene in plant character improvement. The invention discloses a nucleotide sequence of an LsSLR1 gene as shown in SEQ ID NO.3. A recombinant expression vector pCAMBIA1300-GFP-LsSLR1 is constructed, the recombinant expression vector is introduced into a model plant arabidopsis thaliana, and a transgenic arabidopsis thaliana plant over-expressing the LsSLR1 is obtained. Experiments find that overexpression of the LsSLR1 gene can promote development of lateral roots of plants, dwarf the plants, shorten the growth cycle of the plants and improve stress resistance of the plants to salt stress, drought stress or abscisic acid stress, and genetic improvement can be performed on plant traits by using the LsSLR1 gene.

The invention discloses a northern tree seed breeding greenhouse with a monitoring function, and relates to the technical field of agricultural breeding facilities. The northern tree seed breeding greenhouse with the monitoring function comprises a main body, an irrigation unit and a nursery garden, a main body comprises a transparent greenhouse film, a framework and a monitoring assembly, the framework guarantees the structural stability, the monitoring assembly drives a monitoring camera with a built-in near infrared spectrum sensor through a slidable moving cross rod and a telescopic rod, and accurate monitoring of the ABA content of plant bud abscisic acid is achieved; the main body is also provided with an air inlet pipe with an electric heating wire and a main control module integrated with multiple sensors; the irrigation unit is linked with the main control module through an electromagnetic valve to realize precise irrigation; a floor heating module and various soil sensors are arranged in the nursery garden. According to the invention, cooperative monitoring and intelligent regulation and control of the environment in the greenhouse and the plant physiological indexes in the northern cold environment can be realized, and the breeding success rate and efficiency are improved.

The invention discloses a functional gene PaSPL8 for regulating and controlling maturity of apricot fruits. The nucleotide sequence of the gene PaSPL8 is as shown in SEQ ID NO. 1. The functional gene PaSPL8 for regulating and controlling apricot fruit ripening is used for regulating and controlling apricot or tomato fruit ripening, and fruit ripening is delayed by increasing the expression quantity of the gene PaSPL8; or the functional gene PaSPL8 for regulating and controlling the maturity of the apricot fruits is used for regulating and controlling the content of abscisic acid in plant tissues. The invention verifies that the PaSPL8 gene regulates and controls the maturity of apricot fruits by regulating and controlling the ABA content of apricot, so that the PaSPL8 gene can be used for apricot breeding.

The invention discloses a recombinant Yarrowia lipolytica engineering strain for producing abscisic acid and application of the recombinant Yarrowia lipolytica engineering strain, and belongs to the technical field of genetic engineering and bioengineering. According to the invention, heterologous synthesis of ABA is realized in the Yarrowia lipolytica, precursor supply is optimized by enhancing carbon flow in a metabolic pathway, the activity of key enzymes BcABA2 and BcABA3 is improved by adopting a semi-rational strategy, NADPH regeneration engineering is implemented to enhance the oxygen uptake ability of a strain, a cytoplasm-peroxisome engineering system is constructed, and the oxygen uptake ability of the strain is enhanced. The ABA synthesis is further enhanced by utilizing the characteristic of high-yield acetyl coenzyme A of peroxisome. According to the invention, fermentation conditions are further optimized, so that the ABA accumulation concentration of the constructed engineering strain in a 5 L bioreactor reaches 2554.36 mg / L, and the highest titer of ABA biosynthesis reported so far is created. The metabolic modification strategy provides important reference for biosynthesis of other terpenoids.

The invention discloses a functional gene PaACO2 for regulating and controlling maturity of apricot fruits and application of the functional gene PaACO2. The nucleotide sequence of the gene PaACO2 is shown as SEQ ID NO. 1. The PaACO2 gene is used for regulating and controlling the maturation of apricot fruits, and the maturation of the apricot fruits is promoted by increasing the expression quantity of the PaACO2 gene, or the maturation of the fruits is delayed by inhibiting the expression of the PaACO2 gene. The functional gene PaACO2 for regulating and controlling the maturation of the apricot fruits is used for promoting the maturation of the tomato fruits or regulating and controlling the content of abscisic acid and / or aminocyclopropane carboxylic acid in plant tissues. The invention verifies that the PaACO2 gene regulates and controls the maturation of apricot fruits by regulating and controlling the ABA and ACC contents of apricots, so that the PaACO2 gene can be used for breeding apricots to regulate and control the maturation of apricot fruits.

The invention discloses a moso bamboo-derived gene and application of encoded protein thereof, and belongs to the technical field of gene engineering. The gene derived from the moso bamboo comprises a PeDHN4 gene and a PeDHN8 gene; the nucleotide sequence of the PeDHN4 gene is as shown in SEQ ID NO. 15; the nucleotide sequence of the PeDHN8 gene is as shown in SEQ ID NO. 17. According to the PeDHN4 gene and the PeDHN8 gene cloned from moso bamboos, experiments show that the expression of the genes is remarkably up-regulated under the stress of salt, drought and abscisic acid (ABA), and after the genes are introduced into rice through a transgenic technology, the germination rate and the root growth capability of the rice under the stress of salt are remarkably improved. The invention provides key gene resources and technical support for molecular design and breeding of saline-alkaline tolerant crops.

This invention discloses a gene derived from moso bamboo and the application of its encoded protein, belonging to the field of genetic engineering technology. The gene derived from moso bamboo includes the PeDHN4 gene and the PeDHN8 gene; the nucleotide sequence of the PeDHN4 gene is shown in SEQ ID NO.15; and the nucleotide sequence of the PeDHN8 gene is shown in SEQ ID NO.17. The PeDHN4 and PeDHN8 genes cloned from moso bamboo in this invention show that their expression is significantly upregulated under salt, drought, and abscisic acid (ABA) stress. Furthermore, after being introduced into rice using transgenic technology, they significantly improved the germination rate and root growth capacity of rice under salt stress. This invention provides key gene resources and technical support for the molecular design breeding of salt-tolerant crops.

This invention belongs to the field of biotechnology, specifically relating to the cassava transcription factor MeWRKY30 and its applications. This invention cloned the transcription factor gene of MeWRKY30 from cassava. Overexpression of this gene in cassava can improve the resistance of transgenic cassava plants to low-temperature and drought stress. The effects of qRT-PCR on cassava... MeWRKY30 Gene expression was significantly upregulated after drought and treatment with the plant hormone abscisic acid (ABA). After recovery from low temperature treatment, the malondialdehyde content of the overexpressing cassava lines decreased, wilting was reduced, root growth inhibition was lessened, and the number of cortical cells in the root tip meristem was significantly increased.

The invention relates to the technical field of fruit tree cultivation, in particular to a method for thinning kiwi fruits and / or increasing the ascorbic acid content of the kiwi fruits and application. The invention provides an application and method of a plant growth regulator composition in thinning kiwi fruits and / or increasing the ascorbic acid content of kiwi fruits, and the plant growth regulator composition comprises broad beans, ethephon and 6-benayl aminopurine. According to the method disclosed by the invention, the single fruit rate and the ascorbic acid content of the kiwi fruits of Jinchu No.1 can be remarkably increased, and the double-fruit rate of the kiwi fruits is remarkably reduced, but the three-fruit rate and the total fruit setting rate of the kiwi fruits are not influenced.

ABSCISIC ACID SOLVATES AND LIQUID COMPOSITIONS CONTAINING ABSCISIC ACID. The present invention relates to novel modifications of abscisic acid, liquid compositions of abscisic acid, and their use in agrochemical applications. The modifications and compositions of the invention contain abscisic acid in the form of a solvate with at least one N-alkyl pyrrolidone, wherein the N-alkyl group has 3 to 6 carbon atoms. These solvates allow formulations with high concentrations of abscisic acid.

The invention provides application of geranyl linalool to inhibition of growth of lateral roots of notopterygium incisum seedlings and / or breeding of notopterygium incisum superior seedlings, and belongs to the technical field of planting of wild notopterygium incisum seedlings. In the invention, the geranyl linalool can promote the elongation of the roots of the notopterygium incisum seedlings and inhibit the formation of lateral roots by increasing the content of auxin in the notopterygium incisum seedlings and reducing the contents of jasmonic acid, abscisic acid and 1-aminocyclopropane-1-carboxylic acid (an ethylene synthesis precursor). Moreover, the geranyl linalool can also improve the activity of polyphenol oxidase and urease in rhizosphere soil, improve the activity of nitrate reductase and glutamine synthetase in leaves, improve the content of alkali-hydrolyzable nitrogen in soil, reduce the content of total phenolic acid in soil and promote conversion of ammonium nitrogen into nitrate nitrogen, so that nitrogen absorption and utilization are promoted, and growth and shape optimization of seedlings are facilitated. In addition, the geranyl linalool can also improve the abundance of rhizosphere soil probiotic bacteria and reduce the abundance of rhizosphere soil pathogenic fungi, so that the disease resistance of the notopterygium incisum seedlings is improved.

The present invention belongs to the technical field of biological agents, and specifically relates to a Bacillus siamensis DG-39, a bacterial agent thereof, and their application in inhibiting the early bolting of Angelica sinensis. The Bacillus siamensis DG-39 and the bacterial agent containing Bacillus siamensis DG-39 of the present invention mainly inhibit the early bolting of Angelica sinensis by increasing the endogenous melatonin content of Angelica sinensis, decreasing the contents of abscisic acid, auxin and gibberellin, promoting the circulation and utilization of nitrogen, effectively changing the physiological and biochemical state of Angelica sinensis, and down-regulating the expression of photoperiod-related genes. At the same time, the Bacillus siamensis DG-39 bacterial agent of the present invention has the characteristics of nitrogen fixation, non-phosphate solubilization and siderophore production, and can promote the absorption and utilization of nitrogen by plants to avoid the promoting effect of excessive phosphorus on bolting; meanwhile, Bacillus siamensis DG-39 has strong laccase activity and can remove phenolic amine substances that promote bolting, which helps to reduce the bolting rate.

The invention discloses a phyllostachys edulis sourced PeDHN2 gene and application of a coding protein of the phyllostachys edulis sourced PeDHN2 gene, and belongs to the technical field of plant biological breeding. The nucleotide sequence of the PeDHN2 gene is as shown in SEQ ID NO. 11; the sequence of the PeDHN2 gene coding protein is as shown in SEQ ID NO. 12. According to the PeDHN2 gene cloned from moso bamboos, experiments show that expression of the gene is remarkably up-regulated under salt stress, drought and abscisic acid (ABA) stress, and after the gene is introduced into rice through a transgenic technology, the germination rate and root growth ability of the rice under salt stress are remarkably improved. The invention provides key gene resources and technical support for molecular design and breeding of saline-alkaline tolerant crops.

The application of BBX29 gene in negative regulation of plant ABA tolerance and / or salt tolerance belongs to the field of genetic engineering and plant genetic breeding. The application provides the application of BBX29 gene in negative regulation of plant ABA tolerance and / or salt tolerance and a method for negative regulation of plant ABA tolerance and / or salt tolerance. The application can provide a gene resource for cultivating new ABA-tolerant and salt-tolerant varieties, has important significance for genetic improvement of plant salt tolerance and ABA tolerance, and has potential application value.

The invention relates to the technical field of leaf surface conditioner manufacturing, in particular to a compound leaf surface conditioner for improving crop stress resistance and a preparation method and application thereof. The invention provides a composite leaf surface conditioner for improving crop stress resistance. The composite leaf surface conditioner is prepared from 1.8-2.2 parts of abscisic acid, 5-10 parts of Tween 20, 10-15 parts of polyglutamic acid, 5-20 parts of microelement fertilizer and 100 parts of water. Trace elements in the trace element fertilizer comprise at least one of molybdenum, manganese, boron, copper, zinc, iron and calcium. The composite leaf surface conditioner provided by the invention not only improves the stability of ABA, but also obviously improves the stress resistance of crops, ensures nutrient elements required by the crops, promotes the growth of the crops, does not cause secondary pollution, and obviously improves the problem of production obstacles of the crops in saline-alkali soil.

The application belongs to the technical field of biological genetic engineering, and particularly relates to application of a ZmDIR4 gene in regulation of drought sensitivity of corn. The application finds through experiments that after the ZmDIR4 gene is knocked out, the dry matter accumulation of mutant corn plants after drought stress, the activity of antioxidant enzymes in the antioxidant metabolic pathway, the accumulation of proline, chlorophyll and lignan, and the activation degree of the hormone metabolic pathway (zeatin and abscisic acid) are all significantly higher than those of wild type and overexpression materials, proving that the ZmDIR4 gene has a negative regulation function in drought stress resistance of corn. The ZmDIR4 gene can be used to create new corn germplasm with improved drought resistance, and provides a new idea for corn drought resistance.

This invention discloses a functional gene, PaNCED5, that regulates apricot fruit ripening. The nucleotide sequence of the PaNCED5 gene is shown in SEQ ID NO.1. This PaNCED5 gene can be used to regulate apricot fruit ripening by increasing its expression level to promote ripening or by inhibiting its expression to delay ripening. This functional gene, PaNCED5, can also be used to promote tomato fruit ripening or to regulate the content of abscisic acid (ABA) in plant tissues. This invention verifies that the PaNCED5 gene regulates apricot fruit ripening by controlling the ABA content in apricots; therefore, the PaNCED5 gene can be used in apricot breeding.