11952results about "Plant peptides" patented technology

Disclosed herein are methods, compositions, and kits for determining the presence or absence of a target polypeptide (e.g., a polypeptide involved in nitrogen fixation) in a sample (e.g., a sample of soil from the rhizosphere of a non-leguminous plant).

The present invention relates to the fields of genetic engineering and plant genetic breeding, and specifically to the application of wheat TaPND gene. In the present invention, by taking gene TaPND as a target, designing a sgRNA sequence based on CRISPR / Cas9, connecting a DNA fragment containing the encoding sgRNA sequence to a vector carrying CRISPR / Cas, a plant binary expression vector capable of knocking out the TaPND gene in wheat is constructed; by genetically transforming wheat, offspring with TaPND gene knockout are obtained, and wheat mutants with early flowering time are obtained. Experiments have confirmed that transgenic wheat with TaPND gene knockout achieves the advancement of wheat flowering time. The technical scheme of the present invention provides a practical method for realizing rapid wheat breeding using genetic engineering technology, which has important breeding application value and broad market application prospects.

The invention discloses an application of a TaNLP-3B gene and a related biological material thereof in regulating and controlling the protein content of plant seeds. The gene TaNLP-3B is cloned from wheat, the gene TaNLP-3B is overexpressed in wheat Fielder to obtain a TaNLP-3B transgenic overexpressed plant, and a detection result shows that the protein content in wheat seeds can be remarkably increased by more than 40% through TaNLP-3B gene overexpression, so that the TaNLP-3B gene has an effect of regulating and controlling the protein content in the plant seeds, and the protein content in the wheat seeds can be increased by more than 40%. The method can be applied to plant breeding or quality improvement.

The invention discloses a wheat salt-tolerant gene and application thereof. Specifically, the invention discloses a protein TaSGR (SEQ ID NO: 3, 6 and / or 9) and an application of a coding gene of the protein TaSGR in regulation and control of plant salt tolerance and / or plant height. According to the invention, a homozygous gene editing plant of which the TaSGR gene is knocked out is successfully created by utilizing a CRISPR / Cas9 technology. The result shows that after the TaSGR gene is knocked out, the plant height of the wheat is obviously reduced, and the salt tolerance is obviously enhanced. The invention discloses the application of the protein TaSGR and the coding gene thereof for the first time. The salt tolerance of the plant is increased and / or the plant height of the plant is reduced by reducing the content and / or activity of the protein TaSGR (for example, the TaSGR gene is knocked out or silenced). According to the invention, a good gene resource is provided for salt-tolerant breeding of wheat, a new field is developed for application of the TaSGR gene, and the TaSGR gene has important significance for cultivation of novel salt-tolerant wheat.

The invention relates to the field of rice gene engineering, and particularly provides OsSULTR2; the invention also discloses application of the 2 protein and the coding gene thereof in regulating and controlling the salt tolerance of rice. The protein meets the following conditions: B1) a protein with an amino acid sequence of SEQ ID NO.1; and B2) a fusion protein with the same function obtained by connecting a tag to the N end and / or C end of B1). The method is used for detecting OsSULTR2; the salt stress phenotype identification in the seedling stage is carried out on the transgenic rice with the gene knockout 1, 2, and the result shows that when the gene segment is deleted, the salt stress tolerance of the rice is improved, and the function and the application way of the gene are proved. Therefore, the OsSULTR2 of the present invention; the 2 protein and the coding gene thereof can regulate and control the salt tolerance of rice, and have important significance for cultivating salt-tolerant transgenic rice.

According to the barley HvPHR1 gene and the application thereof in the aspect of increasing the phosphorus content of barley, rice PHR2 homologous protein serves as bait, homologous comparison means is utilized, it is found that two PHR proteins HvPHR1 and HvPHR2 exist in barley, and it is found that the content of inorganic phosphorus in barley leaves can be remarkably increased through overexpression of the HvPHR1 gene in an HvPHR1 overexpression strain with the normal phosphorus concentration being 200 micrometers NH4H2PO4 and an HvPHR1 overexpression strain with the low phosphorus concentration being 10 micrometers NH4H2PO4 hydroponic culture for three weeks and a wild type control (GP). In conclusion, the function of the HvPHR1 gene in barley low phosphorus stress is defined, and a theoretical basis is provided for cultivation of new phosphorus-efficient barley varieties.

The invention discloses an AsSN1 gene and application, and relates to the technical field of biology. The nucleotide sequence of the AsSN1 gene is as shown in SEQ ID NO.1, and the amino acid sequence of the AsSN1 protein is as shown in SEQ ID NO.2. The AsSN1 gene and the protein provided by the invention can regulate and control the spikelet number of the oats, further regulate and control the yield of the oats, provide important support for oat breeding and germplasm resource improvement, and have important significance for meeting the development requirements of animal husbandry.

The invention discloses application of a wheat WRKY transcription factor gene TaWRKY1, and belongs to the technical field of gene engineering. A nucleotide sequence of an open reading frame of the gene TaWRKY1 is shown as SEQ ID NO: 1, and the gene TaWRKY1 is infected by stripe rust to induce expression in interaction of wheat and stripe rust, and plays a negative regulation role in wheat stripe rust resistance. A TaWRKY1 gene editing mutant plant is created by using an agrobacterium tumefaciens-mediated genetic transformation method, the sporulation quantity on wheat leaves of the gene editing mutant is obviously reduced, and the allergic necrosis is increased, which indicates that the edited gene TaWRKY1 can improve the resistance of wheat to stripe rust. The invention provides a disease-resistant germplasm resource for the cultivation of the stripe rust-resistant wheat variety.

The invention discloses application of a citrus CsDREB gene in citrus plant height regulation and control and dwarf variety cultivation, and belongs to the technical field of genetic engineering breeding. The invention provides a gene-CsDREB gene which has a remarkable regulation effect on the plant height of citrus, overexpression of the CsDREB gene can remarkably reduce the plant height and internode length of citrus, compared with a wild type, the plant height of a transgenic plant can be averagely reduced by 67.4%, and the higher the relative expression quantity is, the more remarkable the reduction of the plant height is; the CsDREB gene provided by the invention can be used as a candidate gene for breeding and / or breeding dwarf citrus varieties by adopting a genetic engineering method, so that the labor cost of fruit farmers is reduced, the production efficiency is improved, and experimental evidence is provided for clarifying the molecular mechanism of dwarf citrus phenotypes.

The invention relates to a gene GmHB1a participating in development of vascular bundles of soybean root nodules and application of the gene GmHB1a. Specifically, the invention relates to a separated gene participating in development of vascular bundles of soybean root nodules or regulation and control of the number of soybean nodules, and a coding region sequence of the gene is shown as SEQ ID NO: 1 or a homologous sequence of the gene. The invention also provides a method for regulating and controlling the nodulation number of the plant, which comprises the following steps: introducing the gene or a sequence for inhibiting the expression of the gene into a target plant cell or tissue to obtain a transgenic plant, the nodulation number of the transgenic plant is changed compared with that of the target plant, the plant is a leguminous plant, preferably soybean, and the nodulation number of the transgenic plant is changed. Optionally, the change in number of nodules is manifested as an increase in number of nodules and / or in number of single nodules, and optionally, the change in number of nodules is manifested as a decrease in number of nodules and / or in number of single nodules.

The invention provides a Chinese rose heat shock protein family gene RcHSP70-8, the nucleotide sequence of the Chinese rose heat shock protein family gene RcHSP70-8 is shown as SEQ ID No. 1, and the amino acid sequence of the Chinese rose heat shock protein family gene RcHSP70-8 is shown as SEQ ID No. 2. The invention discloses an application of the Chinese rose heat shock protein family gene RcHSP70-8 in delaying flowering of plants, and the RcHSP70-8 protein is positioned in a cell nucleus and can delay flowering through overexpression in arabidopsis thaliana. The invention is helpful for enriching the research on the flowering regulation and control mechanism of the Chinese rose, and has important significance for genetic engineering breeding.

The invention discloses application of ZmRIN4 protein in regulation and control of corn stalk strength, and belongs to the technical field of biological breeding. The technical problem to be solved by the invention is how to regulate and control the stalk strength of plants, for example, how to regulate and control the stalk strength of corns. In order to solve the technical problem, the invention provides a method for increasing the stalk strength and / or stalk diameter of a plant, and the method can be used for increasing or improving the stalk strength and / or stalk diameter of a receptor plant by knocking out ZmRIN4 protein in the receptor plant. The receptor plant is a plant containing a coding gene of the ZmRIN4 protein, and the ZmRIN4 protein is a protein with an amino acid sequence as shown in SEQ ID No. 1. The application discloses the effect of ZmRIN4 in regulating and controlling the strength of corn stalks for the first time; the method can be applied to corn breeding to directionally and accurately improve lodging-resistant and close-planting molecular design breeding of corn, and has a wide market prospect.

The invention discloses an application of a GHERF114 gene in regulating and controlling salt stress tolerance and drought stress tolerance of a plant. The ID (Identity) of the gene is GHA05G2445. According to the invention, an arabidopsis thaliana homozygous line over-expressing the GHERF114 gene is obtained through a transgenic technology; through phenotypic change after salt stress treatment and drought stress treatment, leaf wilting degree, rosette leaf size and form comparison, in-vitro leaf water loss rate, leaf relative water content, chlorophyll content and the like verification, it is indicated that the tolerance of transgenic arabidopsis thaliana to salt stress and drought stress is remarkably reduced, and a TRV: GHERF114 gene silent plant is obtained through a VIGS technology; compared with a control group after salt stress and drought stress treatment, the salt tolerance and the drought resistance of silent plants are remarkably improved. Results show that the GHERF114 plays an important role in plant salt stress and drought stress response processes, has important significance in breeding and research for improving salt tolerance and drought tolerance of cotton, and can be applied to screening of stress-resistant cotton varieties through a gene knockout technology.

The invention discloses a tea tree CsU3 gene promoter and application thereof, and belongs to the technical field of plant biotechnology and gene engineering. Tea tree RNA polymerase III type promoters-tea tree endogenous U3 gene promoters CsU3a, CsU3b, CsU3c and CsU3d are successfully cloned for the first time, a tea tree U3 gene promoter activity detection carrier is successfully constructed, the transcriptional activities of the four CsU3 promoters are compared through instantaneous tobacco leaf conversion, GUS dyeing and GUS enzyme activity determination, and the CsU3 gene promoter activity detection carrier can be used for detecting the activity of the tea tree U3 gene promoter. CsU3a and CsU6b promoters with strong transcriptional activity are selected to be used for constructing a tea tree gene editing carrier, the feasibility of the promoter applied to a tea tree CRISPR / Cas9 gene editing system is verified in a tea tree protoplast, and CRISPR / Cas9 mediated tea tree genome targeted editing is realized.

The invention relates to the field of gene editing, in particular to a peony PoLEC1 gene and application of protein coded by the peony PoLEC1 gene. The peony PoLEC1 gene is cloned from cultivated peony paeonia ostii, after the peony PoLEC1 gene is transformed into arabidopsis thaliana, phenotypic contrast finds that the peony PoLEC1 gene can play a role in bolting and flowering as well as growth and development of plants, specifically, overexpression of the peony PoLEC1 gene can increase the number of leaves, delay bolting and flowering time and reduce the height of the plants. According to the invention, arabidopsis thaliana is taken as a model plant, and a certain theoretical basis is provided for establishing a complete plant regeneration and genetic transformation system for peony by exploring the functional characteristics of the peony PoLEC1 gene and the expression characteristics of the peony PoLEC1 gene in the whole somatic embryo direct generation process and in each tissue of overexpressed arabidopsis thaliana.

The invention provides application of a transcription factor CsPIF7 gene in regulation and control of theanine synthesis and improvement of tea quality, and belongs to the technical field of agricultural molecular biology. The invention finds that high expression of CsPIF7 can activate expression of TS1 and GS1, so that more theanine can be accumulated in tea leaves; after the expression of CsPIF7 is reduced by a tea tree VIGS technology, the activation and expression of TS1 and GS1 can be reduced, so that the content of theanine in tea leaves is reduced. Furthermore, the tea tree transcription factor CsPIF7 is found to be capable of positively regulating and controlling expression of TS1 and GS1 by utilizing LUC verification, and can be used for regulating and controlling the content of theanine in tea trees and improving the quality of tea leaves.

The invention discloses a gene OsSK8 for regulating and controlling disease resistance of rice as well as an encoding protein and application of the gene OsSK8, the gene is the gene OsSK8, and the sequence of the gene OsSK8 is shown as SEQ ID NO. 1 or SEQ ID NO. 2. A novel rice sheath blight and bacterial blight resistance regulation gene OsSK8 is identified, the OsSK8 gene is subjected to overexpression and knockout, and after resistance identification, it is found that the overexpression of the OsSK8 gene can enhance the resistance of rice to sheath blight and bacterial blight, and the knockout of the gene can reduce the resistance of rice to sheath blight and bacterial blight. The OsSK8 gene positively regulates the resistance level of rice to sheath blight and bacterial blight, so that a new thought and direction are provided for breeding rice varieties with higher disease resistance, and meanwhile, a solid foundation is laid for further exploration in the field of rice disease-resistant breeding.

The present invention relates to the use of MtNAC33 gene and a protein encoded thereby in the cultivation of high-biomass and drought-tolerant alfalfa, belonging to the technical field of plant genetic engineering. The plant protein encoded by MtNAC33 gene has the amino acid sequence set forth in SEQ ID NO: 2, and MtNAC33 gene has the nucleotide sequence set forth in SEQ ID NO: 1. In the present invention, the drought tolerance of Arabidopsis thaliana and alfalfa can be significantly improved by regulating the expression level of MtNAC33 in alfalfa. The phenotypes of delayed flowering, increased leaf-to-stem ratio and more biomass in alfalfa were also developed, which has an important effect for genetic improvement of stress resistance and biomass of forage grass and other crops.

The invention relates to a protein for improving the expression yield of a foreign protein and application of the protein. Wherein the ERV29 protein and the related protein as well as the P180 protein and the related protein can be used as molecular chaperones for improving the expression yield of foreign proteins such as Brazilian sweet and the like; the amino acid sequence of the ERV29 protein is as shown in SEQ ID No. 4; the amino acid sequence of the P180 protein is as shown in SEQ ID No. 6.

The invention belongs to the technical field of gene engineering, and particularly relates to application of a ZmDIR11 gene in regulating and controlling salt stress resistance of corn. After salt stress treatment, it is found that the ZmDIR11 gene mutant corn plant growth vigor and material accumulation are significantly lower than those of a wild type B73 material, an oxidative metabolism regulation path, proline accumulation, chlorophyll synthesis, lignan accumulation and a Na < + > / K < + > transport pathway are inhibited, and related metabolic indexes are significantly lower than those of the wild type B73 material. It is shown that the ZmDIR11 gene positively regulates and controls the salt-resistant metabolic pathway of the corn in the corn, and the salt tolerance of the corn is reduced after the ZmDIR11 gene is mutated. The research provides a new gene resource for corn salt-tolerant breeding by utilizing gene engineering.

The present invention relates to a soybean transcription factor GmERF416 and a coding gene thereof, and a use thereof in regulating a plant yield and / or salt tolerance / alkali tolerance, and further relates to a method for cultivating plants having an increased yield and / or salt tolerance / saline-alkali tolerance, and prepared plants, such as soybeans, having an increased yield and / or salt tolerance / saline-alkali tolerance.

The invention belongs to the field of plant genetic engineering, and discloses application of a vitis amurensis cold-resistant gene VaWRKY65 in plant cold-resistant genetic improvement, the VaWRKY65 gene is a transcription factor separated and cloned from extremely cold-resistant vitis amurensis, and the sequence of the VaWRKY65 gene is shown as SEQ ID NO.1. The invention further discloses a preparation method of the vitis amurensis cold-resistant gene VaWRKY65. The gene is used for constructing an overexpression and interference vector, the overexpression and interference vector is respectively introduced into arabidopsis thaliana and vitis amurensis through agrobacterium tumefaciens-mediated genetic transformation, and biological function verification of the obtained transgenic plant shows that the cloned VaWRKY65 gene has the function of improving the cold resistance of the plant. The discovery of the gene provides a new gene resource for plant stress resistance molecular design breeding and provides a new genetic resource for implementation of green agriculture and water-saving agriculture, and development and utilization of the genetic resource are beneficial to reduction of agricultural production cost and realization of environmental friendliness.

The invention provides a Chinese cabbage clubroot susceptibility related gene BrSWEET14a, a construction method and application, belongs to the technical field of plant genetic engineering, and particularly relates to a Chinese cabbage clubroot susceptibility related gene BrSWEET14a. The CDS sequence of the Chinese cabbage BrSWEET14a is as shown in SEQ ID No. 1 (sequence identifier number 1). The gene is transformed into Col-type arabidopsis thaliana through an agrobacterium flower immersion transformation method, a BrSWEET14a heterologous expression arabidopsis thaliana strain is obtained, and the result shows that T-DNA insertion mutant of the BrSWEET14a homologous gene Atsweet14 is more resistant to clubroot than that of the Col-type arabidopsis thaliana, and heterologous expression of Chinese cabbage BrSWEET14a leads to reduction of disease resistance of arabidopsis thaliana to plasmodiophora brassicae. Therefore, the Chinese cabbage BrSWEET14a is closely related to the clubroot disease, and the gene and the homologous gene of the gene in other cruciferous plants can be applied to breeding of the cruciferous plants and other horticultural plants and have a good application prospect.

The invention discloses a sandalwood SaERF33 protein and / or a SaERF33 gene and application of the sandalwood SaERF33 protein and / or the SaERF33 gene in promoting synthesis of sandalwood essential oil. The amino acid sequence of the sandalwood SaERF33 protein is as shown in SEQ ID NO. 2. According to the invention, the SaERF33 gene is obtained from sandalwood for the first time, the synthesis of sandalwood essential oil can be obviously promoted by overexpressing the SaERF33 gene in sandalwood, and the normal growth of plants is not influenced. The invention provides a new genetic resource for cultivating a new sandalwood variety with high essential oil synthesis efficiency, and has important popularization and application values in the aspects of sandalwood variety cultivation and sandalwood essential oil production.

The invention relates to the technical field of plant molecular breeding, in particular to a wheat grain protein content related protein TaSKP1 and application of a coding gene thereof. The amino acid sequence of the protein TaSKP1 related to the protein content of the wheat grains is shown as SEQ ID No.2 or 3; or the amino acid sequence is an amino acid sequence which is obtained by substitution, deletion or insertion of one or more amino acid residues of the amino acid sequence shown as SEO ID No.2 or 3 and has protein with the same function, and the amino acid sequence is obtained by substitution, deletion or insertion of one or more amino acid residues of the amino acid sequence shown as SEO ID No.2 or 3. By reducing the expression quantity of the protein TaSKP1 related to the wheat grain protein content or knocking out the coding gene of the protein TaSKP1, a wheat variety with high grain protein content can be obtained, a theoretical clue is provided for molecular genetic research of the wheat grain protein content, and an important gene resource is also provided for wheat quality molecular breeding.

The invention discloses a Jiangxi No. 1 CcMYB17 gene as well as an expression protein and application thereof, and belongs to the technical field of plant genetic engineering. The nucleotide sequence of the Jiangxi No. 1 CcMYB17 gene disclosed by the invention is as shown in SEQ ID NO.1, and the amino acid sequence of the expression protein of the Jiangxi No. 1 CcMYB17 gene is as shown in SEQ ID NO.2. The method comprises the following steps: constructing a plant expression vector of the Jiangxi No. 1 CcMYB17 gene; transforming the constructed plant expression vector into a '84K poplar' leaf; and cultivating and screening to obtain a '84K poplar' plant which overexpresses the Jiangxi No. 1 CcMYB17 gene. The poplar leaves of the overexpression Jiangxi No. 1 CcMYB17 gene constructed by the invention are deep in color and show obvious red spots; the total anthocyanin content is obviously increased and is obviously higher than that of a wild type. The invention provides a research basis and a theoretical basis for germplasm improvement of the camphor tree.

The invention discloses application of an OsNLP3 gene, and relates to the technical field of plant genetic engineering, and an amino acid sequence coded by the OsNLP3 gene is shown as SEQ ID NO.2. By constructing an OsNLP3 gene overexpression plant and comparing the OsNLP3 gene overexpression plant with a wild type of the OsNLP3 gene overexpression plant, the increase of the single gene expression quantity of the OsNLP3 gene overexpression plant causes the significant increase of the total protein content of the rice, the invariable characters of the grain length, the grain width, the yield and the like, the genetic effect is significant, and the OsNLP3 gene overexpression plant has huge application potential and prospects for the improvement of the characters of rice varieties and provides a new gene resource for

The invention provides application of an ECT2 gene in regulating and controlling development of secondary xylem of a poplar. Transgenic poplar with overexpressed ECT2 genes is obtained through genetic transformation, it is found that overexpressed ECT2 genes can increase the width of secondary xylem of the poplar, and the nucleotide sequence of the ECT2 genes is shown as SEQ ID NO: 1. The ECT2 gene has a regulation effect on the development of the secondary xylem of the poplar.

The invention relates to the field of biotechnology and plant breeding, and particularly provides a method for fixing rice heterosis by using a DMP gene. Aiming at the problems of low maturing rate and complex operation of the existing apomixis system, the haploid induction functions of the rice OsDMP8 and OsDMP13 genes are verified for the first time, the OsPAIR1, OsREC8, OsOSD1 and OsDMP genes are synchronously knocked out through a CRISPR / Cas9 technology, a'MiMe-OsDMP 'four-gene editing system is constructed, and a new thought is provided for construction of the rice apomixis system. A new solution is provided for fixing heterosis of rice apomixis, the efficiency and quality of agricultural production can be greatly improved, and the method has high economic value and wide application prospects.

The invention discloses a wheat E3 ubiquitin ligase gene RFEL1 and application of the wheat E3 ubiquitin ligase gene RFEL1 in wheat disease resistance, and belongs to the technical field of plant genetic engineering. The invention discloses a nucleotide sequence of a wheat E3 ubiquitin ligase gene RFEL1 as shown in SEQ ID NO. 3. The invention further provides application of the wheat E3 ubiquitin ligase gene RFEL1 or related biological materials thereof. The application comprises any one of the following applications: A1, improving the resistance of wheat to stripe rust; a2, the resistance of wheat to powdery mildew is improved; and A3, the resistance of wheat to leaf rust is improved. The E3 ubiquitin ligase RFEL1 gene provided by the invention not only has broad-spectrum resistance to different stripe rust, but also has resistance to wheat leaf rust and powdery mildew. The E3 ubiquitin ligase RFEL1 gene disclosed by the invention is applied to broad-spectrum resistance breeding of wheat and has important application value.