281 results about "Offspring" patented technology

The invention relates to a composition for identifying epinephelus fuscoguttatus, epinephelus coioides and hybrid offspring and application, and belongs to the field of molecular biology, the composition comprises a pair of primers and a group of probes, and the sequences are shown as SEQ ID NO.1-4. The invention further provides a kit for identifying the epinephelus fuscoguttatus, the epinephelus coioides and the hybrid offspring thereof, and the kit is used for identifying the epinephelus fuscoguttatus and the hybrid offspring of the epinephelus coioides and the hybrid offspring of the epinephelus coioides and the hybrid offspring of the epinephelus coioides. The kit comprises the composition disclosed by the invention. The composition can be used for rapidly identifying epinephelus fuscoguttatus, epinephelus coioides and hybrid filial generation gold and tiger hybrid spots of the epinephelus coioides, the epinephelus coioides and the hybrid filial generation gold and tiger hybrid spots of the epinephelus coioides and the epinephelus coioides and the hybrid filial generation gold and tiger hybrid spots of the epinephelus

The invention belongs to the technical field of genetic breeding, and particularly relates to application of a molecular marker for breast circumference character breeding of grassland short-tailed sheep, the molecular marker is at least one of the following (1)-(3): (1) a nucleotide sequence as shown in SEQ ID NO.1, nucleotide at the 101bp site is A or G; (2) a nucleotide sequence as shown in SEQ ID NO.3, wherein the nucleotide at the 101bp site is C or A; (3) a nucleotide sequence as shown in SEQ ID NO.5, wherein the nucleotide at the 101bp site is T or C; the application refers to any one of the following steps: identifying the chest circumference of the grassland short-tailed sheep; the chest circumference of the offspring of the grassland short-tailed sheep is improved. By detecting the genotype of the molecular marker and selecting a dominant genotype individual as a parent, the average chest circumference of offspring can be effectively improved, and a reliable tool is provided for realizing molecular assisted breeding and precise genetic improvement of the chest circumference character of the grassland short-tailed sheep.

The invention relates to the technical field of genetic breeding, in particular to application of a molecular marker related to daily gain traits of sheep before weaning. The molecular marker is at least one of the following (1)-(2): (1) a nucleotide sequence as shown in SEQ ID NO.1, wherein the nucleotide sequence at the 101bp site is T or C; (2) a nucleotide sequence as shown in SEQ ID NO.3, wherein the nucleotide sequence at the 101bp site is T or C. The application refers to any one of the following (1) and (2): (1) identifying the daily gain of the sheep before weaning; and (2) the daily gain of the offspring of the sheep before weaning is improved. The screened molecular marker influencing the daily gain character of the sheep before weaning can be used for identifying the daily gain character of the sheep before weaning, and the daily gain of offspring before weaning can be improved by selecting a CC genotype individual as a male parent or a female parent.

The invention discloses a male sterility gene ZmUCE4 and application thereof in creating a male sterility line of corn, and belongs to the field of plant biotechnology breeding. According to the invention, site-directed mutagenesis of the ZmUCE4 gene in wild type corn is carried out through a CRISPR / Cas9 gene editing technology, pollen development abnormality and abortion can be caused, complete male sterility is caused, and it is proved that the ZmUCE4 gene has a regulation and control function on corn male reproductive development. Through offspring screening, a sterile line without transgenic components can be obtained, a stable maize male sterile line is created, and the method has important significance on maize male fertility control and hybrid seed production. The invention also designs a functional molecular marker aiming at the obtained uce4 male sterility mutant gene, and has important application value in maize male sterility line cultivation, sterile hybrid seed production and molecular marker-assisted selection.

The invention discloses an oat KASP marker, a fingerprint spectrum and application, and relates to the technical field of molecular breeding. The KASP marker comprises 96 SNP (Single Nucleotide Polymorphism) sites, and the position information and the polymorphism of the KASP marker are shown in a table 1. The invention also provides a gene chip for analyzing the KASP marker and an oat variety characteristic fingerprint spectrum constructed by using the KASP marker, and the KASP marker, the gene chip and the oat variety characteristic fingerprint spectrum provided by the invention can be used for screening, identifying and tracing oat quality. The method can be used for identifying the authenticity of the seeds and the genetic relationship between the parent and offspring of the oat, and has important significance for protecting and improving the germplasm resources of the oat.

The invention belongs to the field of food, and particularly relates to application of a nutritional composition for assisting in improving memory ability. The invention provides application of a nutritional composition in preparation of food for assisting in improving memory ability of offspring. The nutritional composition comprises the following necessary components: active folic acid substances and fucosyllactose, moreover, in the nutritional composition, the mass ratio of the active folic acid substance to the fucosyllactose is (0.0001-0.0015): (0.5-10), the food is ingested by a mother to play the role of assisting in improving the memory ability of the offspring, the mother is in a gestation period and / or a lactation period, and the maternal is in the gestation period and / or the lactation period. The filial generation comprises a fetal-stage filial generation and / or an infant-stage filial generation and an optional child-stage filial generation. A large number of studies find that when active folic acid substances and fucosyllactose which are combined according to a certain proportion are supplemented to a mother at the beginning of the gestation period, the memory ability of offspring can be remarkably improved in an auxiliary mode.

The invention relates to the technical field of genetic breeding, in particular to application of a molecular marker related to the weaning weight character of sheep, the molecular marker is a nucleotide sequence shown in SEQ ID NO.1, and nucleotide at the 101bp site is T or C; the application refers to any one of the following (1) and (2): (1) identifying the weaning weight of sheep; and (2) the weaning weight of the offspring of the sheep is improved. The screened molecular marker influencing the weaning weight character of the sheep can be used for identifying the weaning weight character of the sheep, and the weaning weight of offspring can be improved by selecting a CC genotype individual as a male parent or a female parent.

The invention relates to the field of distant hybridization breeding, and discloses an artificial breeding method for hybrid species of humpback perches and epinephelus fuscoguttatus, which comprises the following steps: selecting healthy humpback perches as female parent fishes, and screening healthy epinephelus fuscoguttatus as male parent fishes; carrying out intensive cultivation on the parents by means of nutrient enrichment and temperature regulation and control, and promoting synchronous development and maturation of gonads of the parents; after the parents reach the breeding state, eggs of the female parent fishes and seminal fluid of the male parent fishes are collected respectively, fertilized eggs are obtained through artificial insemination, incubation management such as temperature control and continuous oxygenation is conducted on the fertilized eggs, and finally hybrid grouper fries are obtained. The crossbreeding application of the humpback perch as the female parent and the epinephelus fuscoguttatus is realized, and the problems that in the traditional hybridization process, parent gonad development is asynchronous, and filial generations cannot develop are effectively solved; the bred hybrid provides technical guarantee for standardization and precision promotion of grouper crossbreeding, and creates favorable conditions for breeding new hybrid lines with excellent characters of parents.

The use of a nutritional composition in the preparation of a food product for assisting in improving the memory ability in offspring, wherein the nutritional composition contains the following essential components: an active folic acid substance and fucosyllactose; and in the nutritional composition, the mass ratio of the active folic acid substance to the fucosyllactose is (0.0001-0.0015):(0.5-10). The food product exerts the effect of assisting in improving the memory ability in offspring, wherein the food product is ingested by a mother, the mother is preganant and / or lactating, and the offspring comprises fetal offspring and / or infant offspring and optionally childhood offspring.

Systems and methods are provided that include using models configured to generate offspring genotypes and a fitness evaluator configured to evaluate fitness of the offspring genotypes.

The invention discloses a plant gene function analysis method (FAGEN), and belongs to the technical field of molecular genetics and functional genomics. The method comprises the following steps: constructing a target gene mutant by using a gene editing technology; hybridizing the mutant and the corresponding wild type as a common parent with a plurality of parents with different genetic backgrounds to construct a pair F1 group; an F2 group is obtained through selfing; and determining phenotypic data of target characters of F1 and F2 populations, and comparing phenotypic differences of filial generations of wild types and mutants under different genetic backgrounds to judge the functions of the target genes. The method has the advantages of simplicity in operation, flexibility in design, short construction period and low cost, and gene functions can be efficiently and accurately analyzed under various genetic backgrounds.

The invention belongs to the technical field of aquatic genetic breeding, and particularly relates to a grass carp disease-resistant character genome breeding method and related application thereof. According to the method, filial generation juvenile fish families produced by non-family grass carp parents from different geographical sources are used for constructing an anti-hemorrhagic disease reference group, the disease-resistant SNP site provided by the invention is used for calculating a genome estimation breeding value of a candidate group, and individuals with high breeding value are relatively high in disease resistance and can be used for breeding disease-resistant improved varieties of grass carp.

The invention relates to the technical field of livestock molecular genetic breeding, in particular to application of a molecular marker for grassland short-tailed sheep body weight character breeding, the molecular marker is a nucleotide sequence as shown in SEQ ID NO.1, and nucleotide at the 101bp site is G or A; the application refers to any one of the following steps: identifying the weight of the grassland short-tailed sheep; the body weight of the grassland short-tailed sheep offspring is improved. The SNP molecular marker obviously related to the body weight character is identified on the 24 # chromosome of the grassland short-tailed sheep for the first time. The molecular marker can be used for genotype detection in a young sheep stage or an embryo stage, and does not need to wait for stable phenotype when the sheep grow to an adult stage, so that the breeding cycle is shortened, and the genetic progress is remarkably accelerated. The technical blank in the field of grassland short-tailed sheep body weight character molecular breeding is filled, and a reliable molecular tool and technical support are provided for efficient, accurate and sustainable breeding of the breed.

The invention relates to the technical field of genetic breeding, in particular to application of a haplotype molecular marker related to mutton sheep weight traits. The haplotype molecular marker is a nucleotide sequence as shown in SEQ ID NO.1 and a nucleotide sequence as shown in SEQ ID NO.3; the nucleotide at the 101bp site of the SEQ ID NO. 1 is C or G; the nucleotide at the 101bp site of the SEQ ID NO.3 is C or G, and the nucleotide at the 101bp site of the SEQ ID NO.3 is C or G; the application refers to any one of the following (1) and (2); (1) identifying the weight of the mutton sheep; (2) increasing the weight of the offspring of the mutton sheep. The haplotype molecular marker provided by the invention can be used for early identification and auxiliary seed selection of weight traits of meat sheep, and the weight level of offspring can be effectively improved by screening individuals of which haplotypes are combined into GGGG as parents.

The invention discloses an X-chromosome specific molecular marker of micropterus salmoides and application of the X-chromosome specific molecular marker. On the basis of a whole genome sequencing technology, the X chromosome specific molecular marker of micropterus salmoides is obtained. Aiming at the molecular marker, the invention also provides a detection primer pair and a detection method of the molecular marker, and by detecting the molecular marker, rapid and accurate screening of YY supermale fish individuals can be realized to obtain a YY supermale fish population. The obtained YY super-male fish group and a proper female fish group are jointly fed and bred, and then the micropterus salmoides all-male fingerling can be obtained. If the YY super-male fish group and the YY pseudo-female fish group are jointly bred and bred, largemouth bass offspring which is all YY super-male fish can be obtained. Compared with a traditional all-male breeding method of the largemouth black bass, the all-male breeding method of the largemouth black bass is beneficial to simplifying all-male breeding steps of the largemouth black bass, shortening breeding time, and beneficial to cultivation of all-male largemouth black bass fingerlings and popularization of single-male breeding of the largemouth black bass.

The invention discloses a breeding method of a new strain of meat dwarf recessive white-feather black-bone chickens, and belongs to the technical field of special high-quality broiler chicken seed production. The novel meat dwarf recessive white-feather black-bone chicken strain bred by the method meets the requirements of the market on black meat, fast growth, feeding space, feed conversion rate and the like; by utilizing the recessive white feather characteristic, the new strain is matched with the black-bone chicken paternal line with other feather colors, the feather color of the offspring chicken is consistent with that of the paternal black-bone chicken, and meanwhile, the consistency of the blackness of the black-bone chicken is ensured. The new strain can also be used as a paternal line or a maternal line to be applied to black-bone chicken production through white-skin high-quality chicken hybridization. When the new strain is applied to hybridization matching, the problem of feather color and blackness differentiation caused by growth speed increase through hybridization can be well solved, and the market requirements of live colored-feather black-bone chickens are met.

The application discloses a G101T SNP related to wheat wet gluten content, which is located at the physical position 80813538 bp of the 6A chromosome in the Chinese spring wheat genome IWGSC RefSeq v1.0, and the genotype is G or T. The application develops a KASP marker and a KASP primer group for identifying the wheat wet gluten content based on the SNP site, and provides a method for identifying or assisting in identifying the wheat wet gluten content, and it is found through the method that the wheat wet gluten content of the G101T SNP site genotype TT is higher than or higher than the wheat wet gluten content of the genotype GG. The application has important theoretical significance and economic value for utilizing molecular marker assisted selection to obtain wheat germplasm or breeding offspring materials with higher wet gluten content.

This invention belongs to the field of molecular genetics and discloses a method and application for controlling fish fertility using in vivo gene editing technology. The applicant, for the first time, cloned a carp-specific U6 promoter and used this promoter to construct the U6 gene. MOVIE gRNA transgenic vectors and water The Cas9 vector allows for the establishment of an in vivo editing system. This strategy enables the heritability of sterility, a reproductive control trait, through hybridization of fertile parents to produce sterile offspring. The operation is highly efficient, simple, and environmentally friendly. Furthermore, since high-copy-rate short, scattered repetitive sequences exist in all fish species, this strategy has broad applicability across species.

The invention discloses a method for breeding Chouioia cunea Yang through spodoptera frugiperda pupae and application of the method in prevention and control of spodoptera frugiperda, and belongs to the field of biological prevention and control of agricultural pests. Aiming at the parasitic process of Chouioia cunea Yang on spodoptera frugiperda pupae, the suitable age of hosts, the processing mode of host pupae, the physiological status of parasitic wasps, the ratio of inoculated wasps to pupae and environmental conditions are determined, and a set of breeding method suitable for indoor large-scale breeding is constructed. The stability of the method in the multi-generation breeding process is verified through continuous generation breeding. Compared with the traditional host hyphantria cunea, the method has the advantages that the development duration of breeding Chouioia cunea by taking spodoptera frugiperda pupae as the host is obviously shortened, and the breeding efficiency is improved on the premise of ensuring high parasitic rate and offspring yield. When the Chouioia cunea Yang bred by the method is released in the field, the grassland spodoptera exigua can be efficiently prevented and controlled, the dosage of chemical pesticides is reduced, and the method has good ecological benefits and application prospects.

This invention discloses a multi-unit composite stress tiered breeding device and method for juvenile large yellow croaker, belonging to the field of aquaculture breeding technology. The device uses a central control unit as its sole core, forming a fully integrated unidirectional closed-loop drive system. Four independent breeding units are connected through a transparent channel with a centrally located aeration head, forming a physical flow-limiting isolation structure. Each unit independently regulates water temperature, salinity, and dissolved oxygen, creating a multi-factor composite stress gradient. The breeding method employs a three-factor linear equal-gradient synergistic progressive setting, utilizing the juvenile fish's autonomous adaptation and avoidance instincts to achieve cross-unit migration and natural selection. Combined with contour recognition and dynamic tracking algorithms, it monitors the fish population status and mortality rate in real time, and identifies superior offspring with comprehensive stress resistance through secondary verification under extreme stress. This invention offers high screening accuracy and automation, increasing the survival rate of superior individuals by over 40%, reducing labor costs by over 60%, and allowing for flexible adjustment of device parameters. It can be extended to the stress resistance breeding of other marine economic fish species.

This invention discloses a molecular marker, primer pair, kit, and its application and method for identifying gynogenetic offspring of red crucian carp induced by blunt snout bream. The nucleotide sequence of the molecular marker is shown in SEQ ID NO.1. Based on resequencing analysis and genome alignment of gynogenetic offspring of red crucian carp induced by blunt snout bream, this invention screens for paternal gene fragments from blunt snout bream and develops primer pairs with blunt snout bream molecular markers and specificity. This effectively identifies gynogenetic offspring of red crucian carp induced by blunt snout bream, effectively avoiding the identification difficulties caused by morphological differences. The method of this invention is simple, rapid, and highly accurate, and can play an important role in the field of fish genetics and breeding.

The application discloses a kind of gradient low temperature domestication, constant low temperature storage method of acrosternum hilare adult, comprising the following steps: (1) 10-15 day old acrosternum hilare adult is placed into storage box, the substrate and armyworm are loaded in the storage box;(2) the storage box is placed into artificial climate chamber and gradient low temperature domestication is carried out, and the gradient low temperature domestication program is: 26 DEG C →20 DEG C domestication 8h →15 DEG C domestication 8h →10 DEG C domestication 8h →6 DEG C domestication 8h →4 DEG C domestication 8h;(3) after domestication, acrosternum hilare adult is placed at 4 DEG C and refrigerated, and refrigeration time is 40-60 days.The application can guarantee normal fecundity and egg hatch rate, and better offspring development and offspring survival rate in its life period by placing acrosternum hilare adult into storage box loaded with substrate and armyworm and carrying out gradient low temperature domestication, and then placing it at 4 DEG C and refrigerating for 40-60 days.

The application belongs to the field of genetic engineering and genetic breeding, and particularly relates to a high-temperature response gene HSP70 expression regulation SNP marker of Crassostrea gigas and application of the SNP marker in identifying high-temperature resistant oyster individuals. The SNP marker is the 251th base in the sequence shown in SEQ ID NO: 2, and the mutation type is C / G (trans-regulation site Marker13973). The application provides a SNP marker which is significantly related to the expression of the HSP70 gene, and the advantage of the application is that the genotype of parent oysters can be identified before seed breeding, and the tolerance of offspring to high-temperature environment is improved. The SNP marker obtained in the research has high reliability and stable results.

The invention relates to the technical field of genetic breeding, and discloses a method for stabilizing the chemical property of a subtropical silkworm mid-line variety, which comprises the following steps: screening an egg circle with the optimal chemical property from a production group to establish an initial group; second-generation frame seed production is obtained through incubation and single moth breeding in combination with comprehensive screening of pupa unified life rate, cocoon quality and offspring hatching rate; single moth breeding screening is repeated twice, and third-generation and fourth-generation frame seed production is obtained; adopting a 4 / 4 moth area mixed breeding method to obtain five-generation frame seed production; then five-generation frame seed production is used as a new starting point, the breeding and screening processes are repeatedly executed, each time 4 / 4 moth area mixed breeding is executed as a breeding cycle, and at least two breeding cycles are continuously executed until a new silkworm strain with the sum of the seed generation rate and the re-egg-out rate stably lower than 0.5% is obtained. The method is used for cultivating new silkworm strains which are stable in chemical property and maintain excellent economic characters and robustness, and provides support for stable development of the silkworm industry.

The invention relates to the technical field of livestock and poultry breeding, in particular to a goat hybridization breeding method capable of achieving complete-cycle nutrition enhancement. The core of the method is that systematic nutrition intervention runs through the whole breeding process. Before hybridization, performing differential nutrition pretreatment on the parents for 60 days: feeding the female parents with a breeding nutrition enhancer added with components such as D-chiro-inositol and N-acetylcysteine, and supplementing sperm motility enhancer containing acetylated L-carnitine and ergothioneine to the male parents. In the gamete treatment stage, the oocytes are subjected to in-vitro maturation culture in a special culture solution containing components such as D-chiro-inositol and a growth differentiation factor 9; after the sperms are subjected to gradient centrifugal screening, completing in-vitro fertilization in an optimized fertilization culture solution; after the embryos develop into blastocysts in the sequential culture system, carrying out laparoscopic transplantation, and carrying out staged precise nutrition culture on the pregnant ewes. According to the method, through multi-level nutrition enhancement, the reproductive capacity of the goats and the growth performance of offspring are effectively improved.

The application belongs to the field of fish development, and particularly relates to a molecular marker for identifying Nibea albiflora sperm-induced Pseudosciaena heteroclita gynogenesis offspring and hybrid offspring, a primer set thereof, a kit and application. The primer set for identifying the molecular marker of the Nibea albiflora sperm-induced Pseudosciaena heteroclita gynogenesis offspring and hybrid offspring comprises primers F and R, the nucleotide sequence of the primer F is shown as SEQ ID NO. 1, and the nucleotide sequence of the primer R is shown as SEQ ID NO. 2. Based on genomic data analysis, the application develops the molecular marker F / R which can effectively identify the Pseudosciaena heteroclita gynogenesis offspring and hybrid offspring in the juvenile stage. The method comprises extracting genomic DNA of parents and offspring, PCR amplification and agarose gel electrophoresis detection, and finally distinguishing the gynogenesis individuals and hybrid individuals by whether the Nibea albiflora parent marker is contained in the electrophoresis band.

The present disclosure relates to deoxyribonucleic acid (DNA) editing agents, and their use in preparing genetically modified cells and birds. The present disclosure further relates to fertile genetically modified avians and genetically modified avian primordial germ cells (PGCs) for producing sterile genetically modified avians (birds) that can serve as surrogate hosts for donor PGCs. The present disclosure further relates to methods for producing fertile avian strains that can produce a population of embryos and offspring, in both sexes, all of which are sterile and viable, and further relates to their subsequent use as sterile surrogate hosts for donor PGCs.

The invention belongs to the technical field of aquatic genome selective breeding, and particularly relates to an edwardsiella tarda-resistant genome selective breeding liquid-phase chip for oplegnathus punctatus and application of the liquid-phase chip. According to the method, 500 core SNP loci highly associated with the Edwardsiella tarda resistance character are screened out from 2415773 high-quality SNPs by obtaining the whole genome SNP marker of the oplegnathus punctatus and combining the three methods of MME-rank, GWAS and PVE, and the low-density liquid phase chip prepared by taking the 500 core SNP loci as the core shows extremely high prediction accuracy in a reference group and an independent verification group and can be used for detecting the Edwardsiella tarda resistance character of the Edwardsiella tarda resistance character of the Edwardsiella tarda resistance character of the Edwardsiella tarda resistance character of the Edwardsiella tarda resistance character. The method is obviously superior to a whole genome set and a uniformly distributed marker set; family verification shows that the family GEBV calculated based on the chip is significantly related to the actual survival rate of the offspring. According to the method, high-precision disease resistance performance prediction is realized at extremely low cost, and a key technical tool and a solution are provided for rapid breeding of disease-resistant improved varieties of oplegnathus punctatus.

The invention discloses a thrip resistance identification device and application. The device comprises an inoculation barrel, a preservation substrate is laid at the bottom in the inoculation barrel, an isolation structure with one or more through holes covers the preservation substrate, and a sealing structure with one or more air holes covers an opening in the top of the inoculation barrel. When the device is used, the whole plant does not need to be cultivated, in-vitro flowering branches can be picked for identification, rapid screening of large-scale germplasm resources can be achieved, and repeatability and consistency of experimental results are good. Meanwhile, when the device is used, buds and functional leaves of in-vitro flowering branches are reserved, the actual feeding part of the Frankliniella occidentalis is simulated, and compared with in-vitro leaf inoculation, the real resistance level can be better reflected. The device has the advantages of easily available materials, low cost and strong operability, can be used for identifying the resistance of the Frankliniella occidentalis of various materials such as chrysanthemum and related wild species, cultivated species, breeding offspring and the like, provides a high-quality parent material for insect-resistant breeding, can be used for researching an insect-resistant mechanism, and is suitable for popularization and application in basic breeding units.

The invention provides a seed production method of a new purple golden crassostrea gigas strain with a high meat yield, and belongs to the technical field of aquaculture. The method comprises the following steps: firstly, preliminarily screening out a class group with two shells which are purple golden and purple mainly, 1-3 golden shells and high meat yield as a core group; the method comprises the following steps: selecting crassostrea gigas individuals with alternately purple and golden shells and high meat percentage as an optimal population by adopting a head cutting selection method, taking the optimal population as a breeding population, and carrying out filial generation breeding according to a conventional shellfish breeding method to obtain a new purple and golden shell strain F1 with high meat percentage; further purifying the purple golden shell character in a family breeding mode to form a second-generation breeding family F2; and then a plurality of generations of continuous purification are carried out to obtain the new crassostrea gigas strain with purple gold color, high meat yield and stable ovum shape. The survival rate, the growth speed and the fatness degree of the new purple golden crassostrea gigas strain with the high meat percentage are greatly improved.