465 results about "Offspring" patented technology

The invention belongs to the technical field of genetic breeding, and particularly relates to a molecular marker influencing sheep weight traits and application thereof, and the molecular marker comprises a site I or a site II. The nucleotide sequence of the molecular marker containing the site I is as shown in SEQ ID NO.1, and mutation from T to C occurs at the 101bp position in the SEQ ID NO.1; the nucleotide sequence of the molecular marker containing the site 2 is as shown in SEQ ID NO.2, and mutation from A to G occurs at the 101bp position in the SEQ ID NO.2. According to the molecular markers influencing the sheep weight traits, the marker I and the marker II can be used for identifying the sheep weight traits, and the offspring weight can be increased by selecting TT or AA genotype individuals as male parents or female parents.

The invention discloses a sugarcane germplasm resource evaluation and breeding method for smart agriculture. The sugarcane germplasm resource evaluation and breeding method comprises the following steps: step 1, collecting materials with wide genetic diversity; establishing an intelligent incubator and a greenhouse, and monitoring environmental parameters by using an Internet of Things sensor; 2, performing data acquisition on the related germplasm resources by using an unmanned aerial vehicle, a robot and a near infrared spectrum technology, and constructing a phenotype database for data management; meanwhile, genotype identification is carried out by combining molecular marker-assisted selection and genome selection technologies, molecular markers of key genes are mined, and the genetic value of related germplasm is evaluated; step 3, constructing a hybrid combination prediction model by using an AI algorithm, optimizing parent matching of the related germplasm and the cultivated sugarcane, monitoring and collecting the filial generation at the same time, and analyzing and screening in combination with smart agriculture; 4, demonstration planting is conducted on the bred excellent sugarcane strain, and application of the excellent strain is improved through government-enterprise-scientific research institution collaborative popularization and in combination with farmer technical training.

The invention relates to a composition for identifying epinephelus fuscoguttatus, epinephelus coioides and hybrid offspring and application, and belongs to the field of molecular biology, the composition comprises a pair of primers and a group of probes, and the sequences are shown as SEQ ID NO.1-4. The invention further provides a kit for identifying the epinephelus fuscoguttatus, the epinephelus coioides and the hybrid offspring thereof, and the kit is used for identifying the epinephelus fuscoguttatus and the hybrid offspring of the epinephelus coioides and the hybrid offspring of the epinephelus coioides and the hybrid offspring of the epinephelus coioides. The kit comprises the composition disclosed by the invention. The composition can be used for rapidly identifying epinephelus fuscoguttatus, epinephelus coioides and hybrid filial generation gold and tiger hybrid spots of the epinephelus coioides, the epinephelus coioides and the hybrid filial generation gold and tiger hybrid spots of the epinephelus coioides and the epinephelus coioides and the hybrid filial generation gold and tiger hybrid spots of the epinephelus

The invention belongs to the field of molecular biology, and particularly relates to a transgenic soybean event LP207-1 and a detection method thereof. The invention provides a nucleic acid sequence for detecting a transgenic soybean event LP207-1, the nucleic acid sequence is selected from one or more of sequences SEQ ID NO: 1-7 or complementary sequences thereof, and the nucleic acid sequence is derived from a plant, a seed or a cell of the transgenic soybean event LP207-1. A representative sample of a seed of the transgenic soybean event LP207-1 is preserved in the China Center for Type Culture Collection on May 23, 2025, and the preservation number is CCTCC NO: P202516. The transgenic soybean event LP207-1 has good resistance to lepidoptera pests and can tolerate agricultural herbicides containing glyphosate and glufosinate-ammonium, economic loss caused by the lepidoptera pests can be avoided, and the yield is not reduced; and the breeding efficiency is enhanced, and the molecular marker can be used for tracking a breeding population and transgenic insertion fragments in offspring of the breeding population. The detection method provided by the invention can quickly, accurately and stably identify the existence of the plant material derived from the transgenic soybean event LP207-1.

The invention relates to an intelligent breeding method for crayfish offspring seeds in a winter fallow field, and belongs to the technical field of aquaculture. In order to solve the problems of dissolved oxygen management lag, high ammonia nitrogen accumulation risk and overlarge energy consumption caused by low utilization rate of winter fallow fields and dependence of water quality regulation and control on artificial experience in the prior art, the method comprises the following steps: deploying a water quality dynamic monitoring system based on the Internet of Things to collect data of water temperature, dissolved oxygen, pH value, ammonia nitrogen and nitrite in real time; the power and the water level of the oxygenation equipment are dynamically adjusted after analysis of the edge calculation module; graded regulation and control are achieved by combining a three-level response mechanism, when the dissolved oxygen content is lower than 6 mg / L or the pH value is abnormal, the power of the nanometer oxygenation device is started to 50%, and when the dissolved oxygen content continuously deteriorates, the irrigation system is linked to inject the water body with the temperature being adjusted in advance. According to the method, through intelligent multi-parameter cooperative control and ecological bait design, the resource utilization rate of the winter fallow field is increased, the survival rate of the young crayfish is increased to 85% or above, and the method is suitable for large-scale and green efficient breeding of the young crayfish in a rice-shrimp rotation mode.

Rosa chinensis gray mold is a main disease in the cultivation process of rosa chinensis, and seriously threatens the quality and yield of cut-flower rosa chinensis. In order to effectively cope with the disaster stress which influences the yield and quality of the Chinese rose, 192 pairs of SSR primers are used in the research to carry out PCR amplification on DNA of parents' Jinmai '' Beijing red 'and 6 randomly selected offspring. 35 pairs of primers show genetic difference between male and female parents. The 35 pairs of primers are further used for carrying out PCR amplification on filial generation DNA, detection is carried out through PAGE electrophoresis, and an electrophoretic band is converted into a 0, 1 matrix for genetic typing. The method comprises the following steps: selecting an optimal subset through correlation regression analysis, and finally successfully screening out three primers, namely, Geno-BC-1, Geno-BC-2 and Geno-BC-3, which are related to gray mold resistance. The discovery provides an important theoretical basis for molecular marker-assisted Chinese rose disease-resistant breeding, and is expected to promote early identification of disease-resistant plants, so that the breeding period is shortened, and the breeding efficiency is improved.

The invention discloses a tetraploid crassostrea gigas parent-child relationship identification method based on SNP sites, and belongs to the technical field of tetraploid crassostrea gigas molecular assisted breeding. The method for identifying the parent-child relationship of tetraploid crassostrea gigas comprises the following steps: (1) sequencing adductor muscles of tetraploid crassostrea gigas to be detected; (2) comparing the reference genome of the crassostrea gigas to obtain the genotype of the specified SNP site of the tetraploid crassostrea gigas; (3) calculating IBS and LOD of the to-be-detected parents and the to-be-detected offspring according to an SNP typing result; and (4) identifying the parent-child relationship between the to-be-detected parent and the filial generation by combining IBS and LOD. The SNP loci have the advantages that 1000 SNP loci for identifying the parent-child relationship of the tetraploid crassostrea gigas are disclosed for the first time, data support is provided for identifying the parent-child relationship of the tetraploid crassostrea gigas, a foundation is laid for developing a liquid phase chip for identifying the parent-child relationship of the tetraploid crassostrea gigas, and the SNP loci have good application prospects.

The invention belongs to the technical field of genetic breeding, and particularly relates to application of a molecular marker for breast circumference character breeding of grassland short-tailed sheep, the molecular marker is at least one of the following (1)-(3): (1) a nucleotide sequence as shown in SEQ ID NO.1, nucleotide at the 101bp site is A or G; (2) a nucleotide sequence as shown in SEQ ID NO.3, wherein the nucleotide at the 101bp site is C or A; (3) a nucleotide sequence as shown in SEQ ID NO.5, wherein the nucleotide at the 101bp site is T or C; the application refers to any one of the following steps: identifying the chest circumference of the grassland short-tailed sheep; the chest circumference of the offspring of the grassland short-tailed sheep is improved. By detecting the genotype of the molecular marker and selecting a dominant genotype individual as a parent, the average chest circumference of offspring can be effectively improved, and a reliable tool is provided for realizing molecular assisted breeding and precise genetic improvement of the chest circumference character of the grassland short-tailed sheep.

The invention provides a method for deducing the abundance of each family in a group based on mixed pool simplified genome sequencing, which comprises the following steps of: (1) establishing a parent 2b-rad library, and extracting SNP (Single Nucleotide Polymorphism) genotypes and sites by using a 2b-rad standard process; combining the SNP genotypes of the 2n parents into genotypes of n parent pairs according to a male parent and female parent relationship; comparing the genotypes of the parent pairs, and determining family specific tags; (2) extracting offspring individual tissues, mixing, and carrying out DNA extraction and 2b-rad library building sequencing on the mixed sample tissues; counting the sequencing depth of all the specific tag sites and calculating the secondary allele frequency of the specific tag of the mixed sample filial generation; and (3) calculating the proportion of each family in the offspring according to the sequencing depth of the specific tag site and the secondary allele frequency of the specific tag of the mixed offspring. According to the method, the overall feature distribution of the family can be accurately evaluated without sequencing each individual, so that the cost is greatly reduced.

Disclosed are a genomic mating method for Huaxi cattle based on whole genome single nucleotide polymorphism (SNP) information and an application thereof. The method includes the following specific steps: step 1, extracting deoxyribonucleic acid (DNA) from to-be-hybridized Huaxi cattle individuals for genotyping; step 2, performing genotype data imputation to obtain high-density chip data; step 3, calculating an additive genetic relationship matrix, utilizing genomic best linear unbiased prediction (GBLUP) to obtain genomic estimated breeding values of five important economic traits of a to-be-hybridized Huaxi cattle population, and calculating a comprehensive selection index of the individuals; and step 4, using a genetic algorithm to construct a population optimal mating combination list. In the present invention, the breeding cost is greatly saved and an inbreeding level of offspring populations is reduced.

The invention relates to the technical field of genetic breeding, in particular to application of a molecular marker related to daily gain traits of sheep before weaning. The molecular marker is at least one of the following (1)-(2): (1) a nucleotide sequence as shown in SEQ ID NO.1, wherein the nucleotide sequence at the 101bp site is T or C; (2) a nucleotide sequence as shown in SEQ ID NO.3, wherein the nucleotide sequence at the 101bp site is T or C. The application refers to any one of the following (1) and (2): (1) identifying the daily gain of the sheep before weaning; and (2) the daily gain of the offspring of the sheep before weaning is improved. The screened molecular marker influencing the daily gain character of the sheep before weaning can be used for identifying the daily gain character of the sheep before weaning, and the daily gain of offspring before weaning can be improved by selecting a CC genotype individual as a male parent or a female parent.

The invention discloses a male sterility gene ZmUCE4 and application thereof in creating a male sterility line of corn, and belongs to the field of plant biotechnology breeding. According to the invention, site-directed mutagenesis of the ZmUCE4 gene in wild type corn is carried out through a CRISPR / Cas9 gene editing technology, pollen development abnormality and abortion can be caused, complete male sterility is caused, and it is proved that the ZmUCE4 gene has a regulation and control function on corn male reproductive development. Through offspring screening, a sterile line without transgenic components can be obtained, a stable maize male sterile line is created, and the method has important significance on maize male fertility control and hybrid seed production. The invention also designs a functional molecular marker aiming at the obtained uce4 male sterility mutant gene, and has important application value in maize male sterility line cultivation, sterile hybrid seed production and molecular marker-assisted selection.

The invention relates to the field of plant breeding, and particularly provides a method for fixing rice heterosis by using an OsZFPP gene. The method comprises the following steps: firstly, constructing an OsZFPP1 gene or OsZFPP2 gene expression cassette driven by an OsECA1 promoter; then, an expression box of rice with three target points of OsPAIR1, OsREC8 and OsOSD1 being knocked out of CRISPR / Cas9 is constructed; integrating the expression cassette into the same vector to transform hybrid rice; screening three-gene homozygous mutation and OsZFPP positive plants, and identifying diploid cloned offspring through flow cytometry and genome sequencing. According to the method, the rice OsZFPP gene is combined with MiMe to provide a novel method for fixing the heterosis of the rice through apomixis, the heterosis of the rice can be successfully fixed through the method, an apomixis system with the high maturing rate can be obtained, and a novel solution is provided for fixing the heterosis of the rice through the apomixis.

The invention belongs to the technical field of gene engineering, and relates to a construction method of a mouse model capable of rapidly degrading MSTN protein, which comprises the following steps: sequentially inoculating a 5'homologous arm, 3xEAAAK, an FKBP1A gene synonymous mutation sequence, 3xEAAAK, a red fluorescent protein mCherry sequence and a 3 'homologous arm on a Donor vector to obtain a Donor recombinant vector; designing gRNA based on a third exon of the Mstn gene, mixing the gRNA, Cas9 protein and a Donor recombinant vector, injecting the mixture into a mouse fertilized egg, and performing in-vitro culture and transplantation to obtain an F0-generation mouse; and hybridizing the F0-generation mouse with a wild type, or continuously breeding the filial generation to obtain the knock-in mouse model. According to the invention, a dTAG targeted protein degradation technology is utilized, the obtained homozygous knock-in mouse is subjected to dTAG administration, and rapid and reversible degradation and removal of MSTN protein can be realized.

The invention discloses an oat KASP marker, a fingerprint spectrum and application, and relates to the technical field of molecular breeding. The KASP marker comprises 96 SNP (Single Nucleotide Polymorphism) sites, and the position information and the polymorphism of the KASP marker are shown in a table 1. The invention also provides a gene chip for analyzing the KASP marker and an oat variety characteristic fingerprint spectrum constructed by using the KASP marker, and the KASP marker, the gene chip and the oat variety characteristic fingerprint spectrum provided by the invention can be used for screening, identifying and tracing oat quality. The method can be used for identifying the authenticity of the seeds and the genetic relationship between the parent and offspring of the oat, and has important significance for protecting and improving the germplasm resources of the oat.

The invention belongs to the field of food, and particularly relates to application of a nutritional composition for assisting in improving memory ability. The invention provides application of a nutritional composition in preparation of food for assisting in improving memory ability of offspring. The nutritional composition comprises the following necessary components: active folic acid substances and fucosyllactose, moreover, in the nutritional composition, the mass ratio of the active folic acid substance to the fucosyllactose is (0.0001-0.0015): (0.5-10), the food is ingested by a mother to play the role of assisting in improving the memory ability of the offspring, the mother is in a gestation period and / or a lactation period, and the maternal is in the gestation period and / or the lactation period. The filial generation comprises a fetal-stage filial generation and / or an infant-stage filial generation and an optional child-stage filial generation. A large number of studies find that when active folic acid substances and fucosyllactose which are combined according to a certain proportion are supplemented to a mother at the beginning of the gestation period, the memory ability of offspring can be remarkably improved in an auxiliary mode.

The invention relates to the technical field of genetic breeding, in particular to application of a molecular marker related to the weaning weight character of sheep, the molecular marker is a nucleotide sequence shown in SEQ ID NO.1, and nucleotide at the 101bp site is T or C; the application refers to any one of the following (1) and (2): (1) identifying the weaning weight of sheep; and (2) the weaning weight of the offspring of the sheep is improved. The screened molecular marker influencing the weaning weight character of the sheep can be used for identifying the weaning weight character of the sheep, and the weaning weight of offspring can be improved by selecting a CC genotype individual as a male parent or a female parent.

The invention relates to the field of distant hybridization breeding, and discloses an artificial breeding method for hybrid species of humpback perches and epinephelus fuscoguttatus, which comprises the following steps: selecting healthy humpback perches as female parent fishes, and screening healthy epinephelus fuscoguttatus as male parent fishes; carrying out intensive cultivation on the parents by means of nutrient enrichment and temperature regulation and control, and promoting synchronous development and maturation of gonads of the parents; after the parents reach the breeding state, eggs of the female parent fishes and seminal fluid of the male parent fishes are collected respectively, fertilized eggs are obtained through artificial insemination, incubation management such as temperature control and continuous oxygenation is conducted on the fertilized eggs, and finally hybrid grouper fries are obtained. The crossbreeding application of the humpback perch as the female parent and the epinephelus fuscoguttatus is realized, and the problems that in the traditional hybridization process, parent gonad development is asynchronous, and filial generations cannot develop are effectively solved; the bred hybrid provides technical guarantee for standardization and precision promotion of grouper crossbreeding, and creates favorable conditions for breeding new hybrid lines with excellent characters of parents.

The invention discloses a method for artificially breeding white moth gathering cotesia sinensis and applying the white moth gathering cotesia sinensis to biological control of fall webworm. According to the method, the key factors such as the optimal age, the optimal bee inoculation proportion and the optimal temperature of the hyphantria cunea larvae which are hosts for artificially breeding the cotesia cunea are determined, the parasitism rate and the offspring number of the cotesia cunea are effectively increased, the breeding period is shortened, and the bee breeding efficiency is improved. Meanwhile, an optimal scheme for prolonging the shelf life of the cotesia sinensis gather through low-temperature storage of the cocoons or newly-emerged adults is also defined. The new natural enemy product breeding application technology provided by the invention is specially applied to biological prevention and control of the fall webworms in the larval stage, not only enriches the types of existing fall webworm natural enemy products, but also provides a more comprehensive solution for biological prevention and control of the fall webworms, can reduce the excessive use of chemical pesticides, and is beneficial to environmental protection. And a powerful technical guarantee is provided for balance and health of an ecological system.

The invention discloses a feed containing astragalus polysaccharide and a preparation method thereof, and belongs to the field of feeds. The astragalus polysaccharide is added into the basic feed, so that the adverse effect of heat stress on the female rabbits can be effectively relieved, the conception rate of the female rabbits, the number of live litters and the average weight of the live litters can be increased, and the breeding economic benefits are improved while the reproductive performance is optimized and the growth of the young rabbits is promoted; the prepared feed can effectively improve the serum hormone level of animals and reduce the inflammatory factor level, meanwhile, the diversity of animal intestinal microorganisms under heat stress is effectively improved, a scientific basis is provided for precise feeding management of domestic rabbits in summer, and meanwhile, an important reference is provided for application of astragalus polysaccharide in the field of livestock and poultry anti-stress.

The invention discloses a culture method for improving sebastes schlegeli fry quality, which comprises the following steps: collecting sebastes schlegeli breeder seed fields in advance, artificially breeding parents by using net cages on the sea, performing nutrition rejuvenation on the parents, and obtaining offspring fry by using a method of manually squeezing fry; reasonable culture density is controlled according to different seedling specifications, and natural plant active ingredients easy to absorb are screened to be matched with common microecologics and the like for use, so that disinfection and drug use are avoided; controlling the flowing speed of aquaculture water in different growth stages. The method integrates nutrition rejuvenation, artificial farrowing, pollution-free water quality regulation and control and offspring seed behavior state regulation and control, solves the problems that parent germplasm is poor, dead offspring seeds or offspring seed activity is insufficient, growth is slow, stress resistance is poor and the like in the offspring seed breeding process, and is high in operation controllability in the breeding period, high in offspring seed survival rate and remarkable in growth promoting immune effect; the method has good prevention and treatment effects on bacterial diseases, realizes pollution-free seedling breeding, can reduce disease occurrence and death rate, reduces feed coefficient, and effectively improves culture economic benefits.

The use of a nutritional composition in the preparation of a food product for assisting in improving the memory ability in offspring, wherein the nutritional composition contains the following essential components: an active folic acid substance and fucosyllactose; and in the nutritional composition, the mass ratio of the active folic acid substance to the fucosyllactose is (0.0001-0.0015):(0.5-10). The food product exerts the effect of assisting in improving the memory ability in offspring, wherein the food product is ingested by a mother, the mother is preganant and / or lactating, and the offspring comprises fetal offspring and / or infant offspring and optionally childhood offspring.

The invention discloses a method for identifying Yushan black pigs by adopting an SNP (Single Nucleotide Polymorphism) molecular marker technology, and particularly relates to the technical field of molecular detection.The method comprises the following steps: S1, sample preparation: collecting any one of ear tissues, tail tips or blood samples of to-be-detected pig individuals, and extracting to obtain genome DNA (Deoxyribose Nucleic Acid); the genome DNA is used as a template, amplification detection is conducted through a first primer pair, a second primer pair, a third primer pair and a fourth primer pair corresponding to the first SNP site, the second SNP site and the fourth SNP site, and the nucleotide sequences of the first primer pair, the second primer pair and the fourth primer pair are sequentially SEQ ID NO.5-SEQ ID NO.12. By detecting the specific single nucleotide polymorphic sites in the Yushan black pig genome and carrying out genetic typing comparison, the Yushan black pigs and the filial generation thereof can be quickly and accurately identified.

The invention provides an SNP (Single Nucleotide Polymorphism) molecular marker for identifying salt tolerance of grass carp and application of the SNP molecular marker. The nucleotide sequence of the SNP molecular marker is as shown in SEQ ID NO: 1, wherein the 503rd base of the sequence is T or C. The SNP molecular marker provided by the invention can be applied to rapid identification of the salt tolerance of grass carp and auxiliary breeding of salt-tolerant grass carp, grass carp parents with excellent salt tolerance can be effectively selected under the same breeding condition by detecting the SNP molecular marker, the SNP molecular marker can be effectively used for molecular marker-assisted breeding of grass carp, and the breeding efficiency is improved. The grass carp parents can be subjected to genotype identification according to actual breeding requirements, the grass carp parents with proper genotypes are selected for breeding, grass carp offspring (fry) with high salt resistance can be obtained, breeding time is saved, cost is low, accuracy is high, the grass carp breeding process is accelerated, and economic benefits of grass carp breeding in the saline-alkaline environment are increased.

The invention discloses pediococcus acidilactici for improving offspring social behaviors of a parent and application thereof, and belongs to the technical field of microorganisms. According to the pediococcus acidilactici CCFM1344 provided by the invention, by intervening neurodysplasia caused by high-fat diet of a parent body, the improvement of hyperactivity and social behaviors of the offspring is specifically shown as follows: (1) hyperactivity disorder, induced by high-fat diet of the parent body, of the offspring in juvenile and adult periods is recovered; (2) restoring parent high-fat diet induced social contact disorders of offspring juveniles and adult periods; (3) reducing the gene expression of the small glial cell M1 type differentiation marker CD86 in the offspring hippocampus; and (4) reducing the gene expression of the macrophage activation marker F4 / 80 in the offspring hippocampus. Through the probiotic preparation containing the pediococcus acidilactici CCFM1344, a new thought and method can be provided for early intervention of the autism spectrum disorder.

The invention relates to the technical field of new silkworm variety breeding, and discloses a method for assisting silkworm variety breeding by parthenogenesis, which comprises the following steps: introducing a parthenogenesis high-incidence material into a target variety, screening individual hybridization recurrent parents retaining target characters or genes, and backcrossing with recurrent parent varieties, excellent individuals with target characters are selected for parthenogenesis homozygous immobilization and bisexual reproduction homozygous immobilization; according to the characteristic that a parthenogenesis high-incidence material is not easy to lose in filial generations due to the parthenogenesis high incidence, the parthenogenesis high-incidence material is hybridized, backcrossed and selfed with a target character variety and a conventional excellent variety, and after backcrossing is completed, parthenogenesis homozygous target character is carried out; target character homozygosis can be realized in a few generations, stable homozygous offspring can be obtained, and the breeding process is expected to be shortened.

The invention relates to application of recognition site mutation of OCT4 in breeding of pig spine number and rib number. The recognition site of the OCT4 gene is at the 98095075bp position of the No.7 chromosome of a pig genome, the basic group is mutated from T to C, and the version number of the genome is Sscrofa11.1; during breeding, the number of spines and the number of ribs of offspring pigs are increased by reserving individuals with the genotype of the recognition site being CC and eliminating individuals with the genotype being TC or TT, the production performance of the bred pigs can be effectively improved, the pig strain with the character of the high number of spines and the number of ribs is obtained, and therefore the production performance of the pigs of the strain is improved, and the breeding cost is reduced. The genetic improvement progress and the breeding progress of the breeding pigs are accelerated.

Systems and methods are provided that include using models configured to generate offspring genotypes and a fitness evaluator configured to evaluate fitness of the offspring genotypes.

The invention discloses a plant gene function analysis method (FAGEN), and belongs to the technical field of molecular genetics and functional genomics. The method comprises the following steps: constructing a target gene mutant by using a gene editing technology; hybridizing the mutant and the corresponding wild type as a common parent with a plurality of parents with different genetic backgrounds to construct a pair F1 group; an F2 group is obtained through selfing; and determining phenotypic data of target characters of F1 and F2 populations, and comparing phenotypic differences of filial generations of wild types and mutants under different genetic backgrounds to judge the functions of the target genes. The method has the advantages of simplicity in operation, flexibility in design, short construction period and low cost, and gene functions can be efficiently and accurately analyzed under various genetic backgrounds.

The present invention relates to a method for obtaining a genetically modified progeny of an avian animal by packaging a gene edited or genetically modified vector in a recombinant adenovirus and injecting into the dorsal aorta of an embryo when PGCs migrate to a developing gonad; compared with the existing disclosed method for transforming the PGCs in vitro, the operation method disclosed by the invention not only can stably and effectively infect the PGCs in vivo, but also has the advantages that more importantly, the probability of generating gene editing offspring is higher, and particularly, the breeding efficiency of cultivating the transgenic chicken offspring with KRT75L4 gene and MSTN gene knockout is higher; and on the other hand, the reporter gene or crispr-cas protein gene does not need to be integrated into the genome of the bird and the non-native protein does not need to be expressed, so that the operability is higher from the commercial level.