156 results about "Weaning" patented technology

The invention belongs to the technical field of molecular biotechnology and molecular markers, and particularly relates to an SNP (Single Nucleotide Polymorphism) molecular marker located on a pig chromosome 13 and related to the right nipple number character and application. The site of the SNP marker is the 132654738th nucleotide site of the 132654738th nucleotide site of the 13th chromosome of the international pig reference genome version 11.1, and the basic group of the site is G or A. By optimizing the dominant alleles of the SNP, the frequency of the dominant alleles can be increased generation by generation, the number of right nipples of pigs can be increased, the lactation capacity of sows can be improved, the weaning survival rate of piglets can be increased, excellent breeding pigs with the characters can be bred, the genetic improvement progress of the pigs can be accelerated, and the economic benefits of breeding of the breeding pigs can be effectively improved.

The invention discloses a molecular marker influencing the early weight of sheep as well as a primer and application of the molecular marker. According to the molecular marker capable of influencing the early weight of the sheep, two complete linkage sites of NC056056.1: g.26656G > A and NC056056.1: g.26473C > T of NR4A1 genes of the sheep, and three genotypes of GGCC, AGCT and AATT are shared. The weaning weight of the AGCT type sheep at the age of 45 days and the average daily gain from newborn to weaning are obviously higher than those of GGCC type sheep. The invention relates to a method for screening an early-stage rapid growth sheep variety (line), which comprises the following steps: detecting the G / A polymorphism of a NR4A1NC056056.1: g.26656 site and the C / T polymorphism of a NC056056.1: g.26473 site of sheep, and selecting AGCT type individuals for breeding. The polymorphic site can be used for early auxiliary selection of sheep growth traits, the selection accuracy is improved, and the breeding process is accelerated.

The invention provides an SNP (Single Nucleotide Polymorphism) molecular marker related to weaning weight of a Yondeng seven goat as well as a detection kit and application of the SNP molecular marker, and belongs to the technical field of SNP molecular markers. The nucleotide sequence of the SNP molecular marker is shown as SEQ ID NO.1, the mutation site is the base of the 5343313 site on the No.3 chromosome of the international sheep genome Oarv4.0 version, the dominant allele of the site is selected by typing the site, and the high weaning weight character of the Yondon seven goat can be identified. On the basis of the SNP molecular marker, the invention further provides a method for identifying the weaning weight character of the Zondeng seven goats and screening or breeding the Zondeng seven goats with the high weaning weight character, and the method is high in accuracy, high in detection speed, low in cost and easy in result interpretation and has important application value in large-scale molecular precise breeding of the Zondeng seven goats.

The invention belongs to the field of biology, and discloses lactobacillus mucosa which is preserved in China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC30899, and the preservation address is Institute of Microbiology, Chinese Academy of Sciences, No.3, No.1 Yard, Beichen West Road, Chaoyang District, Beijing. The lactobacillus is separated from small intestines of piglets, is one of main lactobacillus in digestive tracts of the piglets, and has certain cholate tolerance. The lactobacillus mucosa and the composition can promote the weight, the average daily gain and the weaning weight of 14-day-old suckling piglets by regulating the breast milk of the sows, promote the development of an ileum structure and the secretion of gastric mucus and gastric acid, regulate the 5-HT metabolism of intestinal tracts, and improve the immunity of the piglets. The expression of the HTR subtype in the stomach, the jejunum, the ileum and the colon is differentially regulated, and the indole level is increased by regulating tryptophan metabolism of microorganisms in the stomach, the ileum and the colon to play a role. Meanwhile, the invention further discloses application of the lactobacillus mucosa and the composition.

The invention discloses a feed additive for improving reproductive performance of sows and a preparation method of the feed additive, and particularly relates to the technical field of feed additives, and the feed additive comprises a probiotic composition, an antioxidant, a plant extract, trace elements, a synergist and a carrier. The preparation method comprises the following steps: S1, raw material pretreatment; S2, compound fermentation; S3, microcapsule embedding treatment; and S4, freeze-drying and mixing. The feed additive can significantly shorten the interval from weaning to oestrus of sows, increase the number of live litter birth, increase the weaning survival rate of piglets and optimize the reproductive performance, and the preparation process can effectively release traditional Chinese medicine active ingredients, guarantee stable and accurate release of the active ingredients, also bring significant economic benefits, increase the yield of piglets, reduce the cost and improve the economic benefit. The feed has outstanding environmental protection benefits, reduces waste of wastes and feeds, and reduces environmental pressure.

Pentadecanoic acid is added to feed, so that the inflammation level and oxidative stress of animals can be effectively relieved, the weaning stress of the animals is reduced, and the growth and development level of the animals is promoted. The present invention provides a use of pentadecanoic acid in reducing the weaning stress of the animals. Pentadecanoic acid can replace antibiotics to be added to the feed of the animals and is used for maintaining the health level of the animals and promoting the growth and development of the animals.

The invention discloses an application of alpha-ketoglutaric acid (alpha-KG) in preparation of a feed additive for relieving skeletal muscle development injury of pregnancy heat stress induced offspring calves. According to the additive, sodium alpha-ketoglutarate (C5H4O5Na2) accounting for 0.1% of dry matter is uniformly mixed into dry dairy cow total mixed ration (TMR), and the dairy cow is fed with heat stress (THIgt; 72) pregnant Holstein cows, the weight of fetal cattle is increased by 23.2%, and the weight of skeletal muscles is increased by 31.3%. Meanwhile, during pregnancy, alpha-KG is fed, so that the weight of the offspring weaned calves is increased by 10.5%, the weight of skeletal muscles is increased by 28.6%, and the fiber density of the skeletal muscles is improved. According to the method, the skeletal muscle growth and fiber density of the offspring calves are subjected to targeted cross-generation regulation and control through maternal nutrition intervention for the first time, the regulation and control bottleneck of heat stress cross-generation injury in the prior art is broken through, and a solution is provided for improving animal health and growth potential for ruminant heat stress management.

The invention provides a feed for relieving weaning stress of lambs and a preparation method of the feed, and belongs to the field of animal feeds. The feed disclosed by the invention is prepared from the following components in parts by weight: 35-40 parts of puffed corn, 15-20 parts of enzymolysis soybean meal, 5-8 parts of fish meal, 5-8 parts of alfalfa meal, 0.3-3 parts of an organic trace element compound, 0.1-2 parts of a vitamin compound, 3-5 parts of whey powder, 0.5-1 part of butyrin, 0.3-0.5 part of glutamine and 0.2-0.3 part of a radix astragali-spina date seed compound extract. 0.2 to 0.3 part of xylooligosaccharide-xylanase compound, 0.05 to 0.1 part of pancreatin active peptide and 0.1 to 2 parts of complex microbial inoculant. The weaning stress of the lambs is reduced through a targeted intestinal tract-immunity-nerve regulation ternary synergistic formula, the digestive system development of the weaned lambs can be obviously improved, the activity of digestive enzymes can be improved, and therefore the weight gain of the lambs is obviously improved, and the lambs stably pass through the weaning period.

The invention discloses a structural variation molecular marker located on a pig chromosome 7 and related to the lactation ability of a sow. The structural variation molecular marker is a DNA (deoxyribonucleic acid) fragment which is inserted between the 112024896 bp site and the 112024896 bp site on a chromosome 7 of an international pig reference genome 11.1 version, wherein the nucleotide sequence of the DNA fragment is shown as SEQ ID NO: 1; the genotype of the gene is T / T, T / INS and INS / INS. The structural variation molecular marker provided by the invention obviously influences the total weight gain character and the lactation ability character of the 7-day-old sow, and by breeding the pig with the structural variation molecular marker genotype of T / T, the lactation ability of the sow can be obviously improved, and the survival rate and the weaning weight of piglets are increased.

The invention belongs to the technical field of pig molecular markers, and particularly discloses an SNP (Single Nucleotide Polymorphism) molecular marker related to a pig weaning litter number character and application of the SNP molecular marker. Gene typing is carried out by utilizing a gene chip technology, whole genome association analysis is carried out on the basis of SNP molecular markers, and 11 SNP molecular marker sites related to the weaning litter number character of the binary hybrid sow are screened out through the analysis. The sites of the SNP molecular marker are located on a WDR25 gene segment and a BEGAIN gene segment of a pig chromosome 7, and the SNP molecular marker can be used for marker-assisted breeding of the pig weaning litter number character.

The invention relates to the technical field of pig feeds, and particularly discloses a special compound feed for weaning sows, the feed comprises a functional additive, the functional additive comprises the following raw materials in parts by weight: 1-3 parts of a plant extract compound, 2-5 parts of conjugated linoleic acid, 0.0003-0.0005 part of selenomethionine, 0.15-0.35 part of vitamin E, and 0.15-0.3 part of vitamin C; the plant extract compound is prepared from the following components: astragalus polysaccharide, icariin, epigallocatechin gallate and a carrier, and the mass ratio of the astragalus polysaccharide to the icariin to the epigallocatechin gallate is 1 to (1 to 4) to (0.2 to 0.8). The special compound feed for the weaned sows is used for feeding the weaned sows through a scientific and reasonable formula, can promote follicular development of the sows after weaning, improve the oestrus rate and reduce the proportion of non-oestrus sows, and can solve the problem of slow ovarian function recovery of the sows in summer high-temperature seasons.

The invention relates to the technical field of biological breeding, and discloses a composite microecological preparation for adjusting lamb intestinal flora and relieving weaning stress, the composite microecological preparation comprises, by weight, 10-40 parts of enterococcus faecalis powder, 10-40 parts of bacillus subtilis powder and 10-40 parts of saccharomycetes powder, the enterococcus faecalis powder is prepared through a gradient acid-resistant pre-domestication system, and the bacillus subtilis powder is prepared through a gradient acid-resistant pre-domestication system. The bacillus subtilis powder is prepared through an oxygen-controlled induced spore strengthening process, the saccharomycetes powder is prepared through a beta-glucan strengthening reactor, and the three kinds of bacterium powder are mixed under the sterile condition to obtain the bacillus subtilis powder, the saccharomycetes powder and the beta-glucan strengthening reactor. The tolerance, activity and stability of the strain are improved, the controllable and repeatable preparation process of the compound microecological preparation is ensured, and a reliable technical basis is provided for regulating intestinal flora of weaned lambs and relieving weaning stress.

The invention belongs to the technical field of molecular biotechnology and molecular markers, and particularly relates to an SNP (Single Nucleotide Polymorphism) molecular marker located on a pig chromosome 14 and related to a left nipple number character and application. The site of the SNP marker is the 92941574th nucleotide site on a chromosome 14 of an international pig reference genome version 11.1, and the basic group of the site is G or A. By optimizing the dominant alleles of the SNP, the frequency of the dominant alleles can be increased generation by generation, the number of left nipples of pigs can be increased, the lactation ability of sows can be improved, the weaning survival rate of piglets can be increased, excellent breeding pigs with the characters can be bred, the progress of genetic improvement of the pigs can be accelerated, and the economic benefit of breeding of the breeding pigs can be effectively improved.

The invention belongs to the technical field of feed additives and preparation thereof, and discloses a preparation method and application of a hemerocallis citrina baroni extract for promoting lactation of sows, and the preparation method comprises the following steps: S1, selecting a hemerocallis citrina baroni raw material; s2, mixing the day lily with ethanol or pure water with the volume fraction of 80-95% according to the material-liquid ratio of (1: 5)-(1: 10), and performing reflux extraction; s3, combining the extracting solutions, filtering to remove impurities, and concentrating into thick paste; and S4, carrying out spray drying or vacuum drying treatment on the concentrated solution, and crushing the dried material to obtain daylily extract powder. The extracts are obtained from different parts of the day lily through a water extraction or alcohol extraction process, are rich in flavonoid and hydroxy fatty acid active ingredients, can significantly improve the lactation performance of sows and the weight of weaned piglets, and realize feed additive and industrial popularization and application in the field of animal husbandry.

An Acidaminococcus fermentans strain and use thereof in improving rumen fermentation are provided, and relates to the technical field of microorganisms. An acid-producing strain of Acidaminococcus fermentans P47 is obtained by screening, which has desirable passage stability and safety. The strain can increase a ratio of butyric acid in the rumen of a ruminant, improve rumen fermentation parameters of a pre-weaning calf, promote rumen fermentation, improve a rumen environment, and increase an average daily gain (ADG) of the ruminant.

The embodiment of the utility model discloses a weaning device. The weaning device comprises a ring body arranged on the head of a livestock in a sleeving mode; the ring body extends downwards to form the two connecting rods, and the two connecting rods extend to the two sides of the mouth of the livestock respectively; the sector-ring-shaped blocking piece is connected with the end, away from the ring body, of the connecting rod and is perpendicular to the connecting rod; the blocking piece comprises a supporting part formed by outwards extending one end, far away from the ring body, of the connecting rod, and the supporting part is perpendicular to the connecting rod; the end, connected with the connecting rod, of the supporting part is a connecting end, and the end, away from the connecting rod, of the supporting part is a blocking end. The blocking part is used for connecting the two blocking ends; the connecting part is used for connecting the two connecting ends and is positioned between the nose and the mouth of the livestock; and the supporting part, the blocking part and the connecting part enclose to form a sector ring. The weaning device can prevent the mouth of the livestock from getting close to the nipples of the mother of the livestock, can prevent the mother of the livestock and the livestock from being hurt, and does not affect the livestock to eat other food.

The invention provides a breeding-promoting lactating sow compound feed as well as a preparation method and application thereof. The breeding-promoting lactating sow compound feed comprises the following components in parts by weight: 70-110 parts of a base material, 1-2 parts of calcium salt, 1-3 parts of a functional additive and 1-2 parts of a premix, the functional additive is prepared from the following components in parts by weight: 20 to 40 parts of plant extract, 0.2 to 2 parts of verbascoside, 0.1 to 1 part of geniposide and 0.5 to 2 parts of L-theanine. The compound feed provided by the invention can improve the feed intake of the lactating sows, maintain proper backfat thickness, reduce the weaning loss of the sows and improve the weaning oestrus rate of the sows; excessive free radicals generated by the body can be removed, the balance of a redox system in the body is maintained, oxidative stress is prevented from being generated, the reproductive system of the lactating sow is prevented from being damaged, and the reproductive performance of the sow is improved; and inflammatory factors in the sows can be removed, inflammatory reaction of the sows is avoided, and the reproductive capacity of the lactating sows is improved.

The multifunctional supplementary feeding fence for the lambs before weaning relates to the field of the lambs and comprises a supporting bottom plate, moving wheels are symmetrically connected to the bottom face of the supporting bottom plate in a butt joint mode, brake plates are arranged on the side edges of the moving wheels, a side channel is formed in one side edge of the supporting bottom plate, a temperature adjusting rotary knob is connected to the inner side edge of the side channel in an inserted mode, and the temperature adjusting rotary knob is connected to the other side edge of the supporting bottom plate in a butt joint mode. The top face of the supporting bottom plate is in butt joint with an isolation top plate in the vertical direction, the top face of the supporting bottom plate is in butt joint with a matched partition plate in the vertical direction, bottom screw holes are symmetrically formed in the bottom face of the supporting bottom plate, connecting screw rods are fixedly inserted into the top faces of the moving wheels, and inserting top grooves are symmetrically formed in the top face of the supporting bottom plate. A split splicing type structure is adopted, so that the lamb breeding device is small in size after being disassembled to be stored, carried and used, and meanwhile, a temperature-controllable and isolation protection type structure is combined, so that the lamb breeding device can feed and treat lambs more comprehensively.

The invention discloses a method for establishing a rat artificial feeding model, and belongs to the technical field of biomedicine. The method specifically comprises the following steps: 1) selecting a plurality of newly born SD suckling rats, and averagely dividing the rats into an artificial feeding group and a breast feeding group; 2) preparing artificial milk; each SD suckling mouse in the artificial feeding group is artificially fed through the suckling mouse feeding device; (3) the SD suckling rats and the female rats in the breast feeding group are placed in the same space, and manual intervention is not carried out; and (4) the artificial feeding group and the breast feeding group continue to the weaning period, a weight growth curve of the SD suckling rats is drawn, and if the weight growth curve of the artificial feeding group and the breast feeding group have no obvious statistical difference and the death rate is lower than 0.1%, the artificial feeding suckling rat model is successfully established. The model provides feasible animal model support for research on health outcomes of different life development stages of individuals influenced by environmental factors in early life, and can deeply discuss a potential mechanism of influence of milk powder feeding and breast feeding on individual growth and development.

The invention relates to the technical field of microorganisms, in particular to giant spheroidin Escherichia and application of the giant spheroidin Escherichia in promoting intestinal development of piglets. The Megasphaera elsdenii provided by the invention is preserved in Guangdong Microbial Culture Collection Center on May 22, 2025, the preservation number is GDMCC No: 66369, and the Megasphaera elsdenii can be used for promoting the development of intestinal tracts and intestinal mucosa of piglets and retarding growth retardation caused by weaning stress. In addition, the in-vitro pure culture supernatant of the giant spheroidin can also improve the viability of the jejunum stem cells and promote the proliferation of the epithelial cells of the pig intestinal tract.

Method of feeding young pigs involves feeding young pigs with complete mixed feed. Wherein, isoleucine levels in the composition of mixed feed are specifically regulated by introducing synthetic isoleucine. The ratio of standardized ideal digestible (SID) isoleucine to lysine in the diet is 0.55. The specified ratio is maintained in the diets of young pigs during the nursery after weaning, from 24 to 70 days of age.

The invention relates to the technical field of biomedicine, in particular to a piglet weaning diarrhea in-vitro multicellular co-culture model as well as a preparation method and application thereof, and the piglet weaning diarrhea in-vitro multicellular co-culture model provided by the invention is obtained by co-culturing small intestine endothelial cells and IPEC-J2 cells and inducing through an inflammatory factor inducer. The piglet weaning diarrhea in-vitro multi-cell co-culture model provided by the invention solves the problems of incomprehensiveness of a single-cell model and heterogeneity and heterogeneity of an existing model, establishes a cell model closer to the physiological status of intestinal tracts, and is of great significance to research on piglet weaning diarrhea.

The invention provides a breeding method for improving the reproductive performance of sows, and the breeding method comprises the following steps: (1) adding a functional nutritional composition into a sow feed from two weeks before delivery to the day of delivery of the sows; (2) feeding the sow with functional fat powder from 3 days before delivery to 1-3 days after delivery; and (3) from the postpartum period to the weaning period of the sow, adding the functional nutrient composition into the sow feed. The breeding method provided by the invention can relieve the production stress of the sows, reduce postpartum inflammation of the sows, promote rapid postpartum recovery of the sows, shorten the interval of weaning oestrus of the sows and improve the reproductive performance of the sows.

The present invention discloses a method for establishing an artificial feeding model for rats, which belongs to the field of biomedical technology. Specifically: 1) select a number of newly born SD suckling mice and divide them into an artificial feeding group and a breast feeding group on average; 2) prepare artificial milk; artificially feed each SD suckling mouse in the artificial feeding group through a suckling mouse feeding device; 3) the SD suckling mice and the mother mice in the breast feeding group are placed in the same space without artificial intervention; 4) the artificial feeding group and the breast feeding group are continued until the weaning period, and the weight growth curve of the SD suckling mice is drawn. If there is no significant statistical difference between the weight growth curve of the artificial feeding group and the breast feeding group, and the mortality rate is less than 0.1%, the artificial feeding suckling mouse model is successfully established. This model provides a feasible animal model support for exploring the impact of environmental factors in early life on the health outcomes of individuals at different life development stages, and can deeply explore the potential mechanism of the impact of breastfeeding on individual growth and development compared with formula feeding.

The invention relates to the field of breeding feed, and provides a formula of fermented feed for feeding two-end black pigs, and the fermented feed comprises the following preparation materials: 150-220 parts of ice clara; 100 to 180 parts of dried ear grains; 0-30 parts of moso bamboo powder; 0 to 100 parts of straw powder; 200 to 300 parts of rice bran; 180 to 250 parts of corn flour; 90 to 180 parts of soybean meal; 10 to 5 parts of pine needle meal; and 5-10 parts of a fermentation strain. The fermented feed in the formula of the fermented feed for feeding the two-end black pigs is rich in probiotics and organic acid, the intestinal microecological balance can be improved, the weaning litter weight and the weaning weight gain of piglets can be remarkably improved through the EM bacteria fermented feed, meanwhile, the daily average feed intake of sows is remarkably improved, and the diarrhea rate of the piglets is remarkably reduced.

The invention discloses application of 2-hydroxy-4-methylpentanoic acid in preparation of a product for relieving weaning stress, and belongs to the technical field of feed development. The product is a product for improving slow growth caused by weaning stress, promoting weight gain of animals and improving animal intestinal injury caused by weaning stress. The 2-hydroxy-4-methylpentanoic acid directly intervenes in the fundamental pathological change of jejunum epithelium structure damage, an efficient and safe scheme for relieving weaning stress is provided for animal husbandry, and the 2-hydroxy-4-methylpentanoic acid can be applied to piglets and other animals prone to being affected by weaning stress on a large scale and has remarkable economic and social benefits.

The invention discloses a compound traditional Chinese medicine for preventing piglet diarrhea and a use method of the compound traditional Chinese medicine, and relates to the field of traditional Chinese veterinary medicine preparation. 11 to 14 parts of fructus gardeniae; 10 to 15 parts of radix aconiti lateralis preparata; 30 to 36 parts of cyperus rotundus; and 11-14 parts of meadowrue root and rhizome. According to the compound traditional Chinese medicine and the use method thereof, the intestinal microbial flora structure and the intestinal tissue structure of piglets can be improved, and the immune function and the anti-stress capability are remarkably improved, so that the diarrhea rate of the piglets is remarkably reduced, the daily gain and the weaning weight of the piglets are increased, and the growth performance of the piglets is effectively improved.

The invention belongs to the technical field of molecular biology and molecular markers, and particularly relates to an InDel molecular marker located on a porcine chromosome 11 and related to effective right papillary number character and application of the InDel molecular marker. The InDel site of the InDel marker is the 6990232 nucleotide site to the 6990235 nucleotide site on the 11 # chromosome of the international pig reference genome version 11.1, and the basic group of the site is CCAG or C. By optimizing the dominant alleles of the InDel, the frequency of the dominant alleles can be increased generation by generation, the number of effective right nipples of pigs can be increased, the lactation capacity of sows can be improved, the weaning survival rate of piglets can be increased, excellent breeding pigs with the characters can be bred, the progress of genetic improvement of the pigs can be accelerated, and the economic benefit of breeding of the breeding pigs can be effectively improved.

The invention provides application of carvacrol in preparation of a feed additive and / or feed for repairing intestinal injury of piglets, and belongs to the technical field of animal feed. The invention discloses application of carvacrol in preparation of a feed additive and / or feed for repairing intestinal injury of piglets. Experiments show that weaning stress causes piglet growth performance reduction and intestinal barrier injury; the feed added with CAR can effectively repair intestinal injuries of piglets, maintain the integrity of intestinal barriers and regulate the intestinal microbial structure, and carvacrol also has the activity of resisting the characteristics of porcine epidemic diarrhea virus and porcine delta coronavirus. The invention provides a reasonable feeding strategy and theoretical support for piglet intestinal injury caused by weaning stress.

The utility model provides a multifunctional calf feeding device, belongs to the technical field of calf feeding, and solves the problem that in the prior art, weaned calves can only be fed, and feeding is troublesome. The device comprises a box body, a feed storage cavity and a controller are arranged in the box body, a fluid director is arranged in the feed storage cavity, a discharge port is formed in the box body, a feed trough is arranged on the outer side of the box body, the highest horizontal position of the feed trough is higher than the horizontal position of the discharge port, a milk tank is arranged below the feed storage cavity, and the milk tank is connected with a milk outlet pipe extending out of the box body. The milk outlet pipe is connected with a nipple, a first electromagnetic valve is arranged in the milk outlet pipe, and a camera is arranged on the side wall of the box body. The feed storage cavity can output feed for feeding, and the milk tank can output milk for feeding, so that the feeding device is suitable for feeding suckling calves and weaning calves; and the camera can scan the information of the calf, so that the milk outlet pipe is opened for the suckling calf, and the weaning calf is prevented from drinking milk.