81 results about "Allele" patented technology

The present invention relates to tomato plants comprising two copies of an allele associated with enhanced resistance to ToBRFV infection. The invention also relates to seeds and parts of said plants, such as fruits. The invention further relates to methods of making, identifying and using such seeds and plants.

The invention belongs to the technical field of crop molecular markers, and particularly discloses a molecular marker for identifying the salt tolerance of brassica napus in the germination period and application of the molecular marker. The molecular marker is located at the position of an A02 chromosome scafoldA028707654 of a brassica napus reference genome ZS11.v10, and the allelotype of the molecular marker is wild type C or mutant type CATATT insertion. The invention also provides a specific primer group for detecting the molecular marker, and the salt tolerance (sensitive type or tolerance type) of the brassica napus line can be quickly identified according to the existence of 411bp and 416bp bands through double PCR amplification and electrophoresis detection. The molecular marker is significantly associated with salt tolerance phenotypes, the detection method is rapid, accurate and low in cost, the molecular marker can be used for brassica napus salt tolerance germplasm resource screening and molecular marker-assisted breeding, and the breeding efficiency is significantly improved.

The invention belongs to the technical field of molecular marker development and molecular marker assisted breeding, and particularly relates to a brassica napus salt tolerance related KASP molecular marker and application thereof. The invention discloses novel salt-tolerant allelic mutation of major QTL (quantitative trait loci) of A06 chromosome and C08 chromosome of brassica napus. A molecular marker is obtained through whole genome association analysis. According to the brassica napus salt tolerance related KASP molecular marker disclosed by the invention, the amplification effect of marking SNPA06.1, SNPA06.2 and SNPC08 is optimal, and the SNP variation of salt-tolerant germplasm and sensitive germplasm can be obviously distinguished.

IL33 antagonists, alone or in combination with IL-4R antagonists, can be used to treat or inhibit eosinophilic asthma, eosinophilic COPD, eosinophilic ACOS, and nasal polyps in subjects having one or more risk alleles in intronic IL1RL1 variant rs1420101, in IL33 variant rs1342326, in both, or in variants in linkage disequilibrium therewith.

The present application relates to the field of molecular markers and animal genetic breeding technology, and particularly relates to a SNP marker affecting pig purine base content and application thereof. Based on three test pig populations of American purebred Landrace, American purebred Large White and American purebred Duroc, the present application uses pig whole genome resequencing and GWAS analysis to research and determine 15 SNP markers significantly affecting pig purine base content. By selecting the advantageous alleles of the above SNP markers, the frequency of the advantageous alleles can be increased generation by generation, the corresponding purine content can be reduced or increased, the progress of pig genetic improvement can be accelerated, and the economic benefits of pig breeding can be effectively improved.

The invention relates to methods of identifying and characterizing a Cannabis spp. plant with respect to a flower to leaf ratio trait comprising genotyping the plant for a quantitative trait locus (QTL) associated with a flower to leaf ratio trait, and to methods of producing plants having a flower to leaf ratio trait of interest based on defined allelic states of polymorphisms defining the QTL. Also provided are Cannabis spp. plants having a flower to leaf ratio trait of interest comprising defined allelic states of polymorphisms defining the QTL and plants identified, characterized or produced by the methods described herein. The invention further relates to marker assisted selection and marker assisted breeding methods, in particular using a combination of specific markers provided, for obtaining plants having a flower to leaf ratio trait of interest or for modulating the flower to leaf ratio of cannabis plants.

The invention discloses a wheat haploid accurate identification and breeding method based on molecular markers, and belongs to the technical field of agricultural biology. The method comprises the following steps: hybridizing a created multifunctional haploid induction line HIP-Plus serving as a male parent with a target female parent, and saving immature embryos to obtain seedlings; a three-stage identification system comprising visual preliminary screening, molecular confirmation and early selection is adopted to carry out accurate screening on seedlings: firstly, preliminary screening is carried out based on embryo color, then haploid confirmation and transgenic component detection are carried out by utilizing TaMTL allele specific markers, and finally genetic typing is carried out through important character function markers to screen out elite haploids; and carrying out chromosome doubling to obtain a double haploid homozygous line. According to the method, the haploid identification accuracy is improved, the breeding period is shortened, the early directional selection of the target agronomic traits in the haploid stage is realized, and the breeding efficiency and accuracy are greatly improved.

The application belongs to the technical field of cotton molecular breeding, and particularly relates to a KASP molecular marker associated with the flowering trait of upland cotton and an application thereof. The nucleotide sequence of the molecular marker is shown as SEQ ID NO. 27. The molecular marker contains a SNP site. The SNP site is located at the 14th bp from the 5' end of the sequence shown as SEQ ID NO. 27. The polymorphism is G / A. The A allele is a favorable allele for promoting early flowering. The application also develops a primer set for detecting the molecular marker. The early flowering germplasm resource of upland cotton can be screened, the flowering time of upland cotton can be predicted, or early flowering varieties of upland cotton can be assistedly selected by using the primer set. The application also develops a method for assisting in the selection of early flowering varieties of upland cotton. The method can shorten the breeding cycle of the flowering trait of cotton and improve the breeding efficiency of the early flowering trait.

To provide new plants exhibiting improved resistance to nematodes, seeds and parts of the plants, methods of making and using such seeds and plants, and new SmD1 alleles that result in modified SmD1 proteins associated with such improved resistance to nematodes.SOLUTION: Provided are plants comprising SmD1 alleles encoding SmD1 proteins having at least 90% amino acid sequence identity to a particular sequence, wherein the SmD1 proteins comprise missense mutations resulting in modified SmD1 proteins that confer enhanced nematode resistance.SELECTED DRAWING: None

The invention belongs to the technical field of plant bioengineering, and discloses a KASP primer group for detecting wheat plant height and yield synergistically improved QTL locus QPH-6A and application, the primer group is designed for SNP loci at the physical positions 11, 164 and 425 bp of wheat 6A chromosomes, and the primer group comprises two specific forward primers for different alleles and a universal reverse primer. By utilizing the primer group and the detection method provided by the invention, whether the wheat material carries the QPH-6A excellent allele or not can be quickly and accurately identified. The excellent allelic gene has a unique'dwarfing without yield reduction 'genetic effect, and the thousand seed weight, the ear length and the ear seed number can be remarkably increased while the plant height is reduced to a suitable lodging-resistant range (75-85 cm). According to the invention, the problems of too short plant, reduced biomass, premature senility and the like caused by the traditional dwarf gene in arid ecological regions such as Xinjiang are solved, and an efficient breeding tool is provided for cultivating a new high-yield ideal plant type wheat variety suitable for an arid environment.

Plants, cells, tissues, and germplasms comprising genes and labeled alleles associated with increased resistance to gray leaf spot are provided. Also provided are methods of breeding plants having alleles associated with increased resistance to gray leaf spot and methods of identifying and selecting plants having alleles associated with increased resistance to gray leaf spot. Methods for identifying novel genes encoding proteins that provide resistance to gray leaf spot in plants and uses thereof are provided. The genes and marker alleles of the present disclosure can be used to produce gray leaf spot resistant plants by breeding, transgenic modification or genome editing.

A method identifies and selects plants resistant to a fungal disease comprises (a) extraction of nucleic acid from a plant; (b) analysis of extracted nucleic acid for the presence of markers associated with increased fungal resistance within a single chromosome interval; and (c) selection of the plants that have these markers. Furthermore, a method sufficient for introgression into plants of fungal disease resistance alleles comprises (a) crossing parents of plants identified by the first embodiment method with other parents that do not have this resistance; (b) select progenies possessing markers associated with increased resistance to fungal disease using the method as defined in the first achievement; and (c) backcross in one or more cycles the selected progenies with the recurrent genitor to develop new progenies.

A geminivirus replicon, an in planta directed evolution / selection system based thereon, and use. Provided is a geminivirus-based controllable artificial replicon, which comprises two LIRs derived from BeYDV; the two LIRs are located at two flanks of the artificial replicon, respectively. Also provided is an in planta directed evolution / selection system, which comprises the geminivirus-based controllable artificial replicon, a Rep and / or RepA protein, and a mutant or mutant library of a genetic element. The replication of the geminivirus-based controllable artificial replicon is configured to be associated with the desired function of the mutant of the genetic element. The provided in planta directed evolution / selection system is a universal, rapid, and efficient in planta directed evolution system, and can be used for generating new excellent alleles and assisting future agricultural breeding.

The invention belongs to the technical field of plant molecular breeding, and particularly relates to a KASP molecular marker related to soybean aphid resistance and application of the KASP molecular marker. The molecular marker is targeted to a T / G polymorphic SNP (Single Nucleotide Polymorphism) site at 11165119bp of a chromosome 8 in a soybean reference genome Wm82. A2. V1 version; the homozygous type of the G allele can significantly enhance the aphid resistance of soybeans, and is an excellent aphid-resistant genotype. According to the invention, genotypes can be rapidly and accurately distinguished through KASP-PCR fluorescent signal detection, and efficient screening of aphid-resistant soybean individuals is realized. The marker has the advantages of strong correlation of aphid-resistant characters, strong specificity, high detection efficiency and the like, can be widely applied to soybean molecular marker assisted breeding and aphid-resistant germplasm resource evaluation, and provides a key technical tool for breeding aphid-resistant soybean varieties.

Disclosed are recombinant meganucleases engineered to bind and cleave a recognition sequence present in a mutant RHO P23H allele. The invention further relates to the use of such recombinant meganucleases in a method for treating retinitis pigmentosa, wherein the mutant RHO P23H allele is preferentially targeted, cleaved, and inactivated.

The invention provides a molecular marker site related to an initial life heavy character on a Tan sheep chromosome 2, wherein the molecular marker site is SNPs mutation on the Tan sheep chromosome 2. By optimizing the dominant alleles of the markers, the frequency of the dominant alleles can be increased generation by generation, the primary living weight character of the Tan sheep is improved, and the genetic improvement process of the Tan sheep is accelerated, so that the economic benefit of Tan sheep breeding is effectively improved.

The application discloses a SNP molecular marker related to an epididymal weight major quantitative trait locus of a Lake sheep and application thereof, and belongs to the genetic breeding and molecular biology fields.The SNP molecular marker is located at the 33127527th base of a chromosome No.9 of the Lake sheep, and polymorphism of C and T two alleles appears at the site.Different genotypes of the SNP site are significantly related to the epididymal weight of the Lake sheep, and the epididymal weight of an individual with a CC genotype is significantly lower than that of individuals with a TC or TT genotype (P<0.01).The SNP molecular marker is applied to early selection of a high-fertility ram, and is of great significance to improvement of the production capacity of a sheep group and increase of breeding benefits.

This invention discloses a rapid identification method for male sterility loci in rice hybrids, belonging to the field of crop genetics and breeding technology. The method involves purifying F1 hybrid rice pollen by removing impurities and separating fertile pollen through density gradient centrifugation; extracting genomic DNA from fertile pollen and somatic cells of the same plant and performing high-throughput sequencing, using somatic cell sequencing data as background correction; screening for SNPs homozygous in both parents with opposite alleles as ancestral information loci (AIS); calculating allele frequencies based on AIS loci; and locating partial segregation loci using a combination of smooth fitting and maximum likelihood estimation, followed by background signal subtraction to determine the hybrid male sterility locus. This invention eliminates the need for constructing genetic populations and field phenotypic identification, featuring a simple process, short cycle, high throughput, and accurate localization. It can rapidly and efficiently identify male sterility loci in rice hybrids, and is applicable to rice indica-japonica hybrid breeding and the localization and cloning of sterility genes, possessing high application value.

More effective breeding against Cercospora leaf spot or the development of new resistant lines is possible via the provision of Cercospora resistance mediated genes according to the present application; in particular, the dominant resistance effect in the target plant is caused by the properties of the single identified genes. The Cercospora resistance mediated genes as well as the aforementioned embodiments of the present application provide additional applications, for example the use of alleles of the resistance genes in cis- or transgenetic approaches with the aim to develop new resistant cultivars.

The application belongs to the technical field of molecular biology and molecular marker, and particularly relates to a SNP molecular marker related to a pig girth trait on a chromosome 4 of a pig and application thereof. The SNP molecular marker related to the pig girth trait on the chromosome 4, wherein a SNP site corresponds to a T>G mutation at position 122124975 on a chromosome 4 of an international pig reference genome version 11.1, and the polymorphism of the base at the site influences the pig girth trait. The application can increase the frequency of the dominant allele generation by generation by selecting the dominant allele of the SNP, improve the pig girth trait, select the excellent germplasm of the above traits, accelerate the progress of pig genetic improvement, and thus effectively improve the economic benefits of pig breeding.

Provided herein are methods and compositions for producing pea plants having a desirable yield, height, and / or lodging phenotype using marker-assisted selection. The disclosure further provides methods and compositions for introgressing one or more loci comprising at least an allele linked to the yield, height, and / or lodging QTL, thus producing pea plants with a desirable yield, height, and / or lodging phenotype.

The invention discloses a primer pair for identifying wheat with different seed protein contents under a low-fertility soil condition as well as an identification method and application of the primer pair. The primer pair comprises an upstream primer 1 and an upstream primer 2, wherein the nucleotide sequences of the upstream primer 1 and the upstream primer 2 are shown as SEQ ID No. 1 and SEQ ID No. 2; and the nucleotide sequence of the downstream primer is as shown in SEQ ID No.3. Carrying out KASP reaction by using the primer pair and taking the extracted wheat genome DNA as a template, carrying out fluorescence signal reading on a product, and if the signal reading result is Fam allele, determining that the wheat is BB genotype, and if the signal reading result is Fam allele, determining that the wheat is BB genotype; if the result is Hex allele, the wheat is of a bb genotype, and the protein content of the wheat seeds of the BB genotype is greater than that of the wheat seeds of the bb genotype. The primer pair disclosed by the invention can be used for identifying wheat with different seed protein contents under a low-fertility soil condition, and has an important application value in wheat breeding and low-fertility land development and utilization.

The present invention relates to wheat plants comprising a mutation causing an alteration of the amino acid sequence in centromere histone H3 (CENH3), which have the biological activity of a haploid inducer. Further, the present invention provides methods of generating the wheat plants of the present invention and haploid and doubled haploid wheat plants obtainable by crossing the wheat plants of the present invention with wildtype wheat plants.

The application discloses a molecular marker related to wheat stomatal width and application thereof, and belongs to the field of molecular marker breeding.The application develops a KASP molecular marker closely related to the wheat stomatal width based on A / G base variation of a site at 55049899 on a 5D chromosome of the wheat, the nucleotide sequence of the KASP molecular marker is shown as SEQ ID NO.4; further, a primer group for amplifying the KASP molecular marker is designed, including an upstream primer F1, F2 and a downstream primer R connected with different color fluorescent adapters.The experimental results show that the KASP molecular marker developed by the application can efficiently and accurately determine the genetic information of the stomatal width, and predict the stomatal width of the wheat under drought stress, so that the identification cost is saved, the selection efficiency is remarkably improved, the breeding process is accelerated, and technical support is provided for screening of excellent alleles and cultivation of drought-resistant and stress-tolerant wheat varieties.The application has outstanding industrial application value.

The invention discloses a primer group for detecting a KASP molecular marker and application of the primer group in preparation of a preparation for identifying soybean cyst nematode disease, and belongs to the technical field of molecular assisted breeding. The primer group for detecting the KASP molecular marker disclosed by the invention is as shown in SEQ ID NO. 3 to SEQ ID NO. 5. The KASP molecular marker is located at the 8361148bp position of the No.8 chromosome of the soybean reference genome, and the basic group is C / G. The molecular marker is closely linked with soybean cyst nematode disease, and can be used for preliminary screening of soybean cyst nematode disease resistance, so that molecular marker-assisted breeding is achieved, and the breeding process is accelerated. In production, the molecular marker can be used for detecting and screening soybean cyst nematode resistant plants in the seedling stage, the screened germplasm carrying excellent disease-resistant alleles provides a basis for further phenotype identification and rapid screening and utilization of resistant sources, and the yield per unit area of soybeans is increased.

The invention discloses application of a KASP molecular marker in preparation of a preparation for identifying soybean cyst nematode disease, and belongs to the technical field of molecular assisted breeding. According to the application of the KASP molecular marker in preparing the preparation for identifying the soybean cyst nematode disease, the KASP molecular marker is located at the 32969916bp position of the 11th chromosome of a soybean reference genome, and the basic group is C / A. The molecular marker is closely linked with soybean cyst nematode disease, and can be used for preliminary screening of soybean cyst nematode disease resistance, so that molecular marker-assisted breeding is achieved, and the breeding process is accelerated. In production, the molecular marker can be used for detecting and screening soybean cyst nematode resistant plants in the seedling stage, the screened germplasm carrying excellent disease-resistant alleles provides a basis for further phenotype identification and rapid screening and utilization of resistant sources, and the yield per unit area of soybeans is increased.

The application belongs to the field of animal and plant breeding prediction, and discloses a fast and accurate genomic prediction method based on a genetic force model, which calculates the minor allele frequency and linkage disequilibrium score of a marker; obtains an optimal SNP genetic force model through a SNP genetic force model; selects an optimal number of layers; obtains layer genotype data formed by the genotype data of all individuals in a corresponding layer of a to-be-predicted data set; calculates the estimated genetic force of each layer of SNPs based on the optimal SNP genetic force model, and assigns the estimated genetic force to a corresponding diagonal weight matrix of the layer; calculates the kinship matrix between individuals in the corresponding layer genotype data of each layer; fits a multi-random mixed model to obtain the genomic estimated breeding value of each phenotype of all individuals in the to-be-tested data set. The application is based on a linear model framework, has low calculation complexity, and the key steps can be processed in parallel, so the calculation speed is fast; the application constructs a trait-specific SNP genetic force model, optimizes the model representation of the genetic structure of a complex trait, and thus improves the prediction performance.

The application creates new alleles of MTL by a targeted mutagenesis strategy, and is not directly to make MTL lose function, but regulates the expression of MTL in different degrees, or makes individual sequence change, and further improves the fertility and haploid induction rate.

The present application relates to the technical field of molecular marker assisted breeding, and particularly relates to a molecular marker linked to the sex of oyster, a primer pair for detecting the molecular marker and application. The molecular marker is an InDel marker Chr9:8462525, the nucleic acid sequence of which is shown in SEQ ID NO:1, and the primer pair for detecting the molecular marker has nucleotide sequences shown in SEQ ID NO:2 and SEQ ID NO:3. The sex of oyster can be predicted by detecting the molecular marker, and male linkage alleles can be screened, which can be realized by PCR amplification and electrophoresis, and has important application value in breeding unisexual oyster varieties or strains.