256 results about "Allele" patented technology

Among other things, the present disclosure provides various technologies including chirally controlled oligonucleotide compositions and technologies tor manufacturing and using such oligonucleotide compositions. In some embodiments, the present disclosure provides technologies useful for allele -specific knockdown of mutant Huntingtin transcripts. In some embodiments, tire present disclosure provides technologies useful for reducing the expression, level, amount, and / or activity of mutant Huntingtin transcripts or products thereof. In some embodiments, the present disclosure provides methods for treating Huntington's disease.

The invention belongs to the technical field of aquatic animal molecular markers and aquatic genetic breeding, and particularly relates to an SNP (Single Nucleotide Polymorphism) molecular marker remarkably related to growth traits of grass carp and application of the SNP molecular marker. The SNP molecular marker is located at the position of 23608308bp of the eleventh chromosome (SLG1123608308) of the grass carp, and is Tgt; and A basic group mutation. Experiments show that the SNP molecular marker developed by the invention has significant influence on four growth traits (Plt; 0.05), and the allele A has more excellent growth traits. The invention discloses application of an SNP (Single Nucleotide Polymorphism) molecular marker, an amplification primer or a kit in breeding grass carp with excellent growth traits. The SNP molecular marker provided by the invention is not limited by the age, sex and the like of the grass carp, can be used for breeding grass carp varieties with excellent growth traits, and even can be accurately screened after being hatched, so that the breeding process of the grass carp varieties with excellent growth traits is greatly accelerated.

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker linked with a major QTL (Quantitative Trait Loci) related to grass carp body weight traits and application of the SNP molecular marker, the SNP molecular marker is positioned on a 13428777 site basic group of a 24th chromosome of grass carp, and polymorphism of two alleles A and G appears at the site. The screened SNP molecular marker is closely related to the main effect QTL locus of the grass carp body weight character, and plays an important role in regulation and control of the grass carp body weight. When the genotype of the SNP molecular marker is AA, the average body weight and average body length of the grass carp are higher than those of individuals with genotypes of AG and GG. Therefore, the SNP molecular marker can be used for assisting in selection of grass carp breeding, and especially has a wide application prospect when being used for selecting grass carp strains with high growth speed.

The present invention discloses a method for selective crop management in real time. The method comprises steps of: (a) producing a biosensor plant, said biosensor plant comprises a visual biomarker, said biomarker is encoded by at least one modified genetic locus comprising (i) preselected reporter gene allele having a phenotype detectable by a sensor, and (ii) a regulatory region of a preselected gene allele responsive to at least one parameter or condition of said plant or its environment, said regulatory region is operably linked to said reporter gene, such that the expression of said reporter gene phenotype is correlated with the status of said at least one parameter or condition of said biosensor plant or its environment; (b) acquiring image data of a target area comprising a plurality of said biosensor plants via said sensor and processing said data to generate a signal indicative of the phenotypic expression of said reporter gene allele of said biosensor plant; and (c) communicating said signal to an execution unit communicably linked to the sensor, said execution unit is capable of exerting in real time a selective monitoring and / or treatment of said target area or a portion thereof comprising said biosensor plants, said treatment is being responsive to said status of said parameter or condition of the biosensor plant or its environment. The present invention further discloses systems and plants related to the aforementioned method.

The present invention relates to tomato plants comprising two copies of an allele associated with enhanced resistance to ToBRFV infection. The invention also relates to seeds and parts of said plants, such as fruits. The invention further relates to methods of making, identifying and using such seeds and plants.

The invention provides a method for non-invasively evaluating the zygotic property and the fetal fraction of a double-fetal pregnant fetus. Specifically, the method provided by the invention constructs a method for noninvasive evaluation of fetal zygote by detecting and calculating relative abundance of fetal specific allele and fetal specific allele fraction of cfDNA in a blood sample. By adopting the method disclosed by the invention, the zygotic property of the fetus can be efficiently and accurately determined.

The invention discloses a phytolacca americana seed EMS mutation library establishment method, and belongs to the technical field of mutation library construction.The phytolacca americana seed EMS mutation library establishment method comprises the steps that concentrated sulfuric acid is used for pretreating phytolacca americana seeds, then germination accelerating is conducted on the seeds, and the seeds are treated with an EMS solution when the seeds are white, so that the problems that phytolacca americana seed coats are thick, and the sensitivity to EMS is low are solved; according to the mutagenesis method disclosed by the invention, high-density series allele point mutation can be induced and generated, the mutagenesis frequency is relatively high, and meanwhile, chromosome distortion is less caused. In the mutagenesis process, abundant genetic variation can be more efficiently created, and more possibilities are provided for screening mutants with excellent characters. Besides, the method has a remarkable advantage in the aspect of cost, the required chemical reagent is cheap, the experimental operation process is simple, and complicated equipment and technology are not required like common irradiation. The breeding cost is reduced, the working efficiency is improved, and wide popularization and application are facilitated.

The invention discloses an SNP (Single Nucleotide Polymorphism) marker for auxiliary selection of the leaf number of brassica napus, a primer group, a detection method and application, and relates to the technical field of biology and rape breeding. The SNP marker is located at the 9283404 bp position of a brassica napus A02 chromosome, a basic group of the site is a T allele or a C allele, and the T allele is associated with a leafy character. The SNP marker provided by the invention can solve the technical problem that the breeding efficiency of the high-photosynthetic-efficiency ideal-plant-type rape variety is restricted due to the fact that an effective molecular marker closely linked with the leaf number character of the brassica napus is lacked in the prior art and the character cannot be quickly and accurately screened in the early breeding stage.

The present invention relates to methods of breeding with spotted watermelon, Citrullus lanatus, lines and varieties. More specifically, methods of making homozygous-spotted diploid watermelon lines (HSDL) for use in breeding and methods of making homozygous-spotted tetraploid watermelon lines (HSDL) for use in breeding are described. Further, methods of producing 33%, 66%, or 100% spotted triploid hybrid watermelon plants, as well as methods of producing 50% or 100% spotted diploid hybrid watermelon plants, are described. This invention further relates to markers able to determine the percentage of spotted allele present in spotted watermelon lines and varieties. In addition, this invention relates to new and distinctive lines and varieties of watermelon with a spotted phenotype.

The invention relates to the technical field of molecular markers and animal genetic breeding, in particular to an SNP marker influencing pig UMP content and application thereof. Whole genome re-sequencing and GWAS analysis are adopted, American-series purebred landrace pigs, American-series purebred large white pigs and American-series purebred duroc are used as research objects, seven SNP markers related to the pig UMP content are researched and determined, by optimizing dominant alleles of the SNP, the frequency of the dominant alleles can be increased generation by generation, the UMP content can be increased, and the pig UMP content can be improved. The pig genetic improvement progress is accelerated, so that the economic benefit of breeding pig breeding is effectively improved.

The invention discloses an application of an MLANA gene SNP molecular marker in black-bone chicken molecular marker-assisted breeding, the SNP molecular marker is located at the 28230965th site on a Z chromosome and is T or C polymorphism, and a chicken to be bred with a CT dominant genotype is an individual with high huckle muscle black color. The MLANA gene SNP molecular marker is adopted in black-bone chicken muscle black color molecular marker-assisted breeding, a CT dominant allele type can be used as an important molecular marker for breeding a thigh chicken brightness L * value, and breeding for improving thigh muscle black color is assisted by a method of selecting and remaining a CT dominant genotype individual and eliminating other genotype individuals. And the progress of thigh muscle black color generation breeding is accelerated.

Provided is a gene ZmFIE2 for regulating maize kernel development, an encoded protein thereof, an InDel molecular marker, and a use thereof, aiming to solve the current technical problems of low maize haploid induction rate, long breeding cycle, and low maize yield. An InDel molecular marker for detecting ZmFIE2 mutants was screened out, and a primer for InDel molecular marker was designed, the InDel molecular marker and the primer thereof can be applied to the breeding of maize kernel size varieties and maize haploid or diploid lines. From the epigenetic level, it was found that ZmFIE2 regulates the development of maize embryo and endosperm, affects the accumulation of kernel storage materials, and affects the cell cycle process of endosperm cells, which has great value in improving maize yield. By using multiple created ZmFIE2 allele mutants, research has found that the loss of ZmFIE2 function can directly determine the division of endosperm cells and apomixis, which will provide new gene resources and new ways to achieve high-frequency haploid induction and heterosis fixation in maize.

Provided are plants, cells, tissues, and germplasm thereof comprising genes and marker alleles associated with increased resistance to gray leaf spot disease. Also provided are breeding methods and methods of identifying and selecting plants having alleles associated with increased resistance to gray leaf spot. Provided are methods to identify novel genes that encode proteins providing plant resistance to gray leaf spot and uses thereof. The disclosed genes and marker alleles are useful in the production of gray leaf spot disease resistant plants through breeding, transgenic modification, or genome editing.

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker related to the salt tolerance of island cotton and application of the SNP molecular marker. The site of the SNP molecular marker is located at the 45674375th site of a D02 chromosome of gossypium barbadense, and the allele at the 45674375th site is A or G. According to the invention, whole genome association analysis is carried out on the relative plant height and the relative overground part fresh weight in salt stress and salt-free stress environments, the SNP site related to the salt tolerance of the sea island cotton is obtained through screening, and the SNP molecular marker is accurate and efficient in detection and convenient and stable in amplification, can be used for molecular marker-assisted selection, and can be used for detecting the salt tolerance of the sea island cotton. The identification efficiency of different salt-tolerant cotton varieties is improved.

Provided are plants, cells, tissues, and germplasm thereof comprising genes and marker alleles associated with increased resistance to gray leaf spot disease. Also provided are breeding methods and methods of identifying and selecting plants having alleles associated with increased resistance to gray leaf spot. Provided are methods to identify novel genes that encode proteins providing plant resistance to gray leaf spot and uses thereof. The disclosed genes and marker alleles are useful in the production of gray leaf spot disease resistant plants through breeding, transgenic modification, or genome editing.

The invention provides an SNP (Single Nucleotide Polymorphism) molecular marker combination for predicting a Chinese rose flower type and application of the SNP molecular marker combination. The combination comprises 2001 SNP loci distributed on 14 chromosomes of the rosa chinensis, and can be used for predicting the flower types of the rosa chinensis, including circles, irregular circles and stars. The SNP loci are determined through genome-wide association analysis and screening of a plurality of software models, and have dominant alleles which are positively and remarkably associated with the Chinese rose types. The invention also relates to a prediction probe and a prediction chip based on the SNP molecular marker combination, and applications of the prediction probe and the prediction chip in China rose type prediction. According to the SNP molecular marker combination, the prediction probe or the prediction chip, the prediction model is combined, the florescence characteristics of the Chinese roses can be predicted 6-8 months after the Chinese roses are planted, the breeding period is greatly shortened to 2-3 years, meanwhile, the prediction precision is improved, the cost is reduced, the screening efficiency is improved, a variety right technical barrier is formed, and the SNP molecular marker combination, the prediction probe or the prediction chip have important theoretical value and application significance.

The invention belongs to the technical field of gene detection, and particularly discloses an STR fluorescent multiplex amplification detection kit for pigeon individual recognition, a detection method and application of the STR fluorescent multiplex amplification detection kit. The kit comprises 19 STR (short tandem repeat) loci and one sex identification locus of the domestic pigeon, also comprises an allele lader and the like, can more accurately judge the typing of the loci, has high cumulative paternity exclusion rate, can be applied to individual identification and paternity relationship identification of the domestic pigeon, has high detection sensitivity, and can obtain an accurate typing result only by 0.5 ng of DNA (deoxyribonucleic acid).

The invention belongs to the technical field of crop molecular markers, and particularly discloses a molecular marker for identifying the salt tolerance of brassica napus in the germination period and application of the molecular marker. The molecular marker is located at the position of an A02 chromosome scafoldA028707654 of a brassica napus reference genome ZS11.v10, and the allelotype of the molecular marker is wild type C or mutant type CATATT insertion. The invention also provides a specific primer group for detecting the molecular marker, and the salt tolerance (sensitive type or tolerance type) of the brassica napus line can be quickly identified according to the existence of 411bp and 416bp bands through double PCR amplification and electrophoresis detection. The molecular marker is significantly associated with salt tolerance phenotypes, the detection method is rapid, accurate and low in cost, the molecular marker can be used for brassica napus salt tolerance germplasm resource screening and molecular marker-assisted breeding, and the breeding efficiency is significantly improved.

The invention belongs to the technical field of molecular marker development and molecular marker assisted breeding, and particularly relates to a brassica napus salt tolerance related KASP molecular marker and application thereof. The invention discloses novel salt-tolerant allelic mutation of major QTL (quantitative trait loci) of A06 chromosome and C08 chromosome of brassica napus. A molecular marker is obtained through whole genome association analysis. According to the brassica napus salt tolerance related KASP molecular marker disclosed by the invention, the amplification effect of marking SNPA06.1, SNPA06.2 and SNPC08 is optimal, and the SNP variation of salt-tolerant germplasm and sensitive germplasm can be obviously distinguished.

The application belongs to the technical field of molecular breeding of Brassica napus, and particularly relates to a SNP molecular marker related to the thousand seed weight of Brassica napus and application thereof. The SNP molecular marker is located at the position of 43,480,336 of the scaffold C05 chromosome of Brassica napus reference genome ZS11 v0. The site has T / G polymorphism, wherein the T allele is a favorable allele variation for increasing the thousand seed weight of Brassica napus. The SNP molecular marker can be used for screening high thousand seed weight potential materials at the seedling stage or early stage through DNA detection, improving the high yield breeding efficiency of Brassica napus, and can be used for screening high thousand seed weight materials of Brassica napus, molecular assisted selection breeding, evaluation of germplasm resources and improvement of yield-related traits.

The invention discloses a method for cross breeding of trichosanthes kirilowii maxim, which belongs to the technical field of breeding and comprises the following steps: performing sister line inbreeding through female plants and male plants in the same trichosanthes kirilowii maxim, planting seed seedlings according to plant rows in the second year, combining field phenotype identification, eliminating bad single plants, selecting single plant groups with excellent characters, and further performing sister line inbreeding homozygosis to obtain the trichosanthes kirilowii maxim. The method comprises the following steps: continuously inbreeding compulsory sister lines for 3-4 years, eliminating bad allelic genes, identifying the homozygous degree of different sites of a whole genome by using SSR molecular markers to obtain inbred lines with excellent comprehensive characters, calculating genetic distances of different inbred lines according to genotypes, and selecting the inbred lines as parents in combination with phenotypic character complementation and breeding target requirements of the inbred lines. The method comprises the following steps: carrying out cross breeding on snakegourd fruits, selecting single plants with excellent comprehensive characters from filial generations, carrying out propagation through root tuber or tissue culture, and carrying out yield and related quality comparison so as to breed more new snakegourd fruit varieties with high yield, high quality, disease resistance and excellent comprehensive characters.

IL33 antagonists, alone or in combination with IL-4R antagonists, can be used to treat or inhibit eosinophilic asthma, eosinophilic COPD, eosinophilic ACOS, and nasal polyps in subjects having one or more risk alleles in intronic IL1RL1 variant rs1420101, in IL33 variant rs1342326, in both, or in variants in linkage disequilibrium therewith.

The invention relates to the technical field of biomolecular markers, and provides a chicken butyric acid level and / or death rate related SNP marker and application thereof, the SNP marker comprises a 106476774th nucleotide site corresponding to a No.3 chromosome of a GRCg6a-galGal6 genome, and the basic group of the nucleotide site is A or T. The invention also provides an application of the SNP marker in chicken butyric acid level and / or death rate related SNP marker in chicken butyric acid level and / or death rate related SNP marker in chicken butyric acid level and / or death rate related SNP marker in chicken butyric acid level and / or death rate related SNP marker. By optimizing the dominant alleles of the SNP, broilers with the butyric acid level and / or high and low mortality can be rapidly identified, broilers with high mortality can be eliminated, the frequency of the dominant alleles is increased generation by generation through breeding, the butyric acid level and / or mortality of the broilers, especially the 28-42-day-old stage, of the broilers is reduced, the genetic improvement progress of the domestic broilers is accelerated, and the breeding efficiency of the domestic broilers is improved. Therefore, the economic benefit of chicken breeding is effectively improved, and the application value is high.

Provided are a rice mutant gene and the use thereof in regulating the plant height of rice. The rice mutant gene at least has an allele mutation, and the allele mutation is the mutation into Asn from Asp at the 377th site in an amino acid sequence shown as SEQ ID No. 26. The mutant gene can effectively regulate and control the rice plant height, and has good application potential in plant architecture breeding of rice varieties.

The invention discloses molecular marker identification and application of dwarf round-grain wheat alleles. The invention belongs to the field of plant breeding, and particularly relates to molecular marker identification and application of dwarf round-grain wheat alleles. The method for identifying or assisting in identifying the wheat dwarf round grains comprises the steps that the genotype of an SNP site in a wheat genome to be detected is detected, the phenotype of the wheat dwarf round grains is identified or assisting in identifying according to the genotype, the SNP site is an SNP site on a wheat 3B chromosome, the nucleotide type of the SNP site is T or C, and the SNP site is the 185th nucleotide of a sequence 1 in a sequence table.

The invention discloses an application of an MITF gene SNP molecular marker in black-bone chicken muscle and skin black color molecular marker assisted breeding. The SNP molecular marker comprises the following two SNP sites: an SNP1 molecular marker is located at the 15747890th site of a 12 # chromosome positive-sense strand, is in a C or T polymorphism, is numbered as rs316573471, and has a dominant genotype of TT; the SNP2 molecular marker is located at the 15751940th site of a positive-sense strand of a chromosome 12, is A or G polymorphism, and is numbered as rs14045480, and the dominant genotype is AA. By adopting the MITF gene SNP molecular marker in black-bone chicken muscle black color molecular marker assisted breeding, TT and AA dominant allelotype can be respectively used as important molecular markers for breeding thigh muscle brightness L * value and back skin brightness L * value, and TT genotype and AA genotype individuals are selected and reserved; the method of eliminating other genotype individuals is used for assisting in improving the breeding of the huckle muscle black color and the back skin black color, and the progress of the generation breeding of the huckle muscle and the back skin black color is accelerated.

The invention relates to a gene for regulating and responding to environment temperature change and application thereof. Specifically, the invention provides the application of the TOMS1 (Thomson MeSocotyl1) or the allele of the TOMS1 in the aspect of regulating and controlling the rice to respond to the environment temperature change so as to improve the yield. The invention further discloses a method for cultivating the plants suitable for growing at the high environment temperature. The TOMS1 gene has a wide application prospect in high environment temperature adaptation and high-yield plant breeding.

The present application relates to the field of molecular markers and animal genetic breeding technology, and particularly relates to a SNP marker affecting pig purine base content and application thereof. Based on three test pig populations of American purebred Landrace, American purebred Large White and American purebred Duroc, the present application uses pig whole genome resequencing and GWAS analysis to research and determine 15 SNP markers significantly affecting pig purine base content. By selecting the advantageous alleles of the above SNP markers, the frequency of the advantageous alleles can be increased generation by generation, the corresponding purine content can be reduced or increased, the progress of pig genetic improvement can be accelerated, and the economic benefits of pig breeding can be effectively improved.

A method of enhancing grain yield of a Gramineae plant is provided. The method comprises:(a) crossing a recipient plant with a donor plant comprising a sequence variation in a TPR allele, said sequence variation imparting enhanced grain yield; and(b) selecting a progeny plant of said crossing comprising said sequence variation by identifying said variation, said progeny plant being characterized by an improved grain yield relative to said recipient plant. Also provided are plants and processed products resultant of the method.