140 results about "Allele" patented technology

The present invention relates to tomato plants comprising two copies of an allele associated with enhanced resistance to ToBRFV infection. The invention also relates to seeds and parts of said plants, such as fruits. The invention further relates to methods of making, identifying and using such seeds and plants.

The invention discloses an SNP (Single Nucleotide Polymorphism) marker for auxiliary selection of the leaf number of brassica napus, a primer group, a detection method and application, and relates to the technical field of biology and rape breeding. The SNP marker is located at the 9283404 bp position of a brassica napus A02 chromosome, a basic group of the site is a T allele or a C allele, and the T allele is associated with a leafy character. The SNP marker provided by the invention can solve the technical problem that the breeding efficiency of the high-photosynthetic-efficiency ideal-plant-type rape variety is restricted due to the fact that an effective molecular marker closely linked with the leaf number character of the brassica napus is lacked in the prior art and the character cannot be quickly and accurately screened in the early breeding stage.

The present invention relates to methods of breeding with spotted watermelon, Citrullus lanatus, lines and varieties. More specifically, methods of making homozygous-spotted diploid watermelon lines (HSDL) for use in breeding and methods of making homozygous-spotted tetraploid watermelon lines (HSDL) for use in breeding are described. Further, methods of producing 33%, 66%, or 100% spotted triploid hybrid watermelon plants, as well as methods of producing 50% or 100% spotted diploid hybrid watermelon plants, are described. This invention further relates to markers able to determine the percentage of spotted allele present in spotted watermelon lines and varieties. In addition, this invention relates to new and distinctive lines and varieties of watermelon with a spotted phenotype.

The invention relates to the technical field of molecular markers and animal genetic breeding, in particular to an SNP marker influencing pig UMP content and application thereof. Whole genome re-sequencing and GWAS analysis are adopted, American-series purebred landrace pigs, American-series purebred large white pigs and American-series purebred duroc are used as research objects, seven SNP markers related to the pig UMP content are researched and determined, by optimizing dominant alleles of the SNP, the frequency of the dominant alleles can be increased generation by generation, the UMP content can be increased, and the pig UMP content can be improved. The pig genetic improvement progress is accelerated, so that the economic benefit of breeding pig breeding is effectively improved.

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker related to the salt tolerance of island cotton and application of the SNP molecular marker. The site of the SNP molecular marker is located at the 45674375th site of a D02 chromosome of gossypium barbadense, and the allele at the 45674375th site is A or G. According to the invention, whole genome association analysis is carried out on the relative plant height and the relative overground part fresh weight in salt stress and salt-free stress environments, the SNP site related to the salt tolerance of the sea island cotton is obtained through screening, and the SNP molecular marker is accurate and efficient in detection and convenient and stable in amplification, can be used for molecular marker-assisted selection, and can be used for detecting the salt tolerance of the sea island cotton. The identification efficiency of different salt-tolerant cotton varieties is improved.

The invention provides an SNP (Single Nucleotide Polymorphism) molecular marker combination for predicting a Chinese rose flower type and application of the SNP molecular marker combination. The combination comprises 2001 SNP loci distributed on 14 chromosomes of the rosa chinensis, and can be used for predicting the flower types of the rosa chinensis, including circles, irregular circles and stars. The SNP loci are determined through genome-wide association analysis and screening of a plurality of software models, and have dominant alleles which are positively and remarkably associated with the Chinese rose types. The invention also relates to a prediction probe and a prediction chip based on the SNP molecular marker combination, and applications of the prediction probe and the prediction chip in China rose type prediction. According to the SNP molecular marker combination, the prediction probe or the prediction chip, the prediction model is combined, the florescence characteristics of the Chinese roses can be predicted 6-8 months after the Chinese roses are planted, the breeding period is greatly shortened to 2-3 years, meanwhile, the prediction precision is improved, the cost is reduced, the screening efficiency is improved, a variety right technical barrier is formed, and the SNP molecular marker combination, the prediction probe or the prediction chip have important theoretical value and application significance.

The invention belongs to the technical field of crop molecular markers, and particularly discloses a molecular marker for identifying the salt tolerance of brassica napus in the germination period and application of the molecular marker. The molecular marker is located at the position of an A02 chromosome scafoldA028707654 of a brassica napus reference genome ZS11.v10, and the allelotype of the molecular marker is wild type C or mutant type CATATT insertion. The invention also provides a specific primer group for detecting the molecular marker, and the salt tolerance (sensitive type or tolerance type) of the brassica napus line can be quickly identified according to the existence of 411bp and 416bp bands through double PCR amplification and electrophoresis detection. The molecular marker is significantly associated with salt tolerance phenotypes, the detection method is rapid, accurate and low in cost, the molecular marker can be used for brassica napus salt tolerance germplasm resource screening and molecular marker-assisted breeding, and the breeding efficiency is significantly improved.

The invention belongs to the technical field of molecular marker development and molecular marker assisted breeding, and particularly relates to a brassica napus salt tolerance related KASP molecular marker and application thereof. The invention discloses novel salt-tolerant allelic mutation of major QTL (quantitative trait loci) of A06 chromosome and C08 chromosome of brassica napus. A molecular marker is obtained through whole genome association analysis. According to the brassica napus salt tolerance related KASP molecular marker disclosed by the invention, the amplification effect of marking SNPA06.1, SNPA06.2 and SNPC08 is optimal, and the SNP variation of salt-tolerant germplasm and sensitive germplasm can be obviously distinguished.

The application belongs to the technical field of molecular breeding of Brassica napus, and particularly relates to a SNP molecular marker related to the thousand seed weight of Brassica napus and application thereof. The SNP molecular marker is located at the position of 43,480,336 of the scaffold C05 chromosome of Brassica napus reference genome ZS11 v0. The site has T / G polymorphism, wherein the T allele is a favorable allele variation for increasing the thousand seed weight of Brassica napus. The SNP molecular marker can be used for screening high thousand seed weight potential materials at the seedling stage or early stage through DNA detection, improving the high yield breeding efficiency of Brassica napus, and can be used for screening high thousand seed weight materials of Brassica napus, molecular assisted selection breeding, evaluation of germplasm resources and improvement of yield-related traits.

IL33 antagonists, alone or in combination with IL-4R antagonists, can be used to treat or inhibit eosinophilic asthma, eosinophilic COPD, eosinophilic ACOS, and nasal polyps in subjects having one or more risk alleles in intronic IL1RL1 variant rs1420101, in IL33 variant rs1342326, in both, or in variants in linkage disequilibrium therewith.

The invention relates to the technical field of biomolecular markers, and provides a chicken butyric acid level and / or death rate related SNP marker and application thereof, the SNP marker comprises a 106476774th nucleotide site corresponding to a No.3 chromosome of a GRCg6a-galGal6 genome, and the basic group of the nucleotide site is A or T. The invention also provides an application of the SNP marker in chicken butyric acid level and / or death rate related SNP marker in chicken butyric acid level and / or death rate related SNP marker in chicken butyric acid level and / or death rate related SNP marker in chicken butyric acid level and / or death rate related SNP marker. By optimizing the dominant alleles of the SNP, broilers with the butyric acid level and / or high and low mortality can be rapidly identified, broilers with high mortality can be eliminated, the frequency of the dominant alleles is increased generation by generation through breeding, the butyric acid level and / or mortality of the broilers, especially the 28-42-day-old stage, of the broilers is reduced, the genetic improvement progress of the domestic broilers is accelerated, and the breeding efficiency of the domestic broilers is improved. Therefore, the economic benefit of chicken breeding is effectively improved, and the application value is high.

Provided are a rice mutant gene and the use thereof in regulating the plant height of rice. The rice mutant gene at least has an allele mutation, and the allele mutation is the mutation into Asn from Asp at the 377th site in an amino acid sequence shown as SEQ ID No. 26. The mutant gene can effectively regulate and control the rice plant height, and has good application potential in plant architecture breeding of rice varieties.

The invention discloses molecular marker identification and application of dwarf round-grain wheat alleles. The invention belongs to the field of plant breeding, and particularly relates to molecular marker identification and application of dwarf round-grain wheat alleles. The method for identifying or assisting in identifying the wheat dwarf round grains comprises the steps that the genotype of an SNP site in a wheat genome to be detected is detected, the phenotype of the wheat dwarf round grains is identified or assisting in identifying according to the genotype, the SNP site is an SNP site on a wheat 3B chromosome, the nucleotide type of the SNP site is T or C, and the SNP site is the 185th nucleotide of a sequence 1 in a sequence table.

The invention discloses an application of an MITF gene SNP molecular marker in black-bone chicken muscle and skin black color molecular marker assisted breeding. The SNP molecular marker comprises the following two SNP sites: an SNP1 molecular marker is located at the 15747890th site of a 12 # chromosome positive-sense strand, is in a C or T polymorphism, is numbered as rs316573471, and has a dominant genotype of TT; the SNP2 molecular marker is located at the 15751940th site of a positive-sense strand of a chromosome 12, is A or G polymorphism, and is numbered as rs14045480, and the dominant genotype is AA. By adopting the MITF gene SNP molecular marker in black-bone chicken muscle black color molecular marker assisted breeding, TT and AA dominant allelotype can be respectively used as important molecular markers for breeding thigh muscle brightness L * value and back skin brightness L * value, and TT genotype and AA genotype individuals are selected and reserved; the method of eliminating other genotype individuals is used for assisting in improving the breeding of the huckle muscle black color and the back skin black color, and the progress of the generation breeding of the huckle muscle and the back skin black color is accelerated.

The present application relates to the field of molecular markers and animal genetic breeding technology, and particularly relates to a SNP marker affecting pig purine base content and application thereof. Based on three test pig populations of American purebred Landrace, American purebred Large White and American purebred Duroc, the present application uses pig whole genome resequencing and GWAS analysis to research and determine 15 SNP markers significantly affecting pig purine base content. By selecting the advantageous alleles of the above SNP markers, the frequency of the advantageous alleles can be increased generation by generation, the corresponding purine content can be reduced or increased, the progress of pig genetic improvement can be accelerated, and the economic benefits of pig breeding can be effectively improved.

A method of enhancing grain yield of a Gramineae plant is provided. The method comprises:(a) crossing a recipient plant with a donor plant comprising a sequence variation in a TPR allele, said sequence variation imparting enhanced grain yield; and(b) selecting a progeny plant of said crossing comprising said sequence variation by identifying said variation, said progeny plant being characterized by an improved grain yield relative to said recipient plant. Also provided are plants and processed products resultant of the method.

The invention relates to methods of identifying and characterizing a Cannabis spp. plant with respect to a flower to leaf ratio trait comprising genotyping the plant for a quantitative trait locus (QTL) associated with a flower to leaf ratio trait, and to methods of producing plants having a flower to leaf ratio trait of interest based on defined allelic states of polymorphisms defining the QTL. Also provided are Cannabis spp. plants having a flower to leaf ratio trait of interest comprising defined allelic states of polymorphisms defining the QTL and plants identified, characterized or produced by the methods described herein. The invention further relates to marker assisted selection and marker assisted breeding methods, in particular using a combination of specific markers provided, for obtaining plants having a flower to leaf ratio trait of interest or for modulating the flower to leaf ratio of cannabis plants.

The application belongs to the technical field of aquatic animal molecular marker and aquatic genetic breeding, and particularly relates to a SNP molecular marker significantly related to growth traits of grass carp and application thereof. The SNP molecular marker is located at 23608308 bp of the 11th chromosome of the grass carp (SLG11_23608308), and is a T>A base mutation. Experiments show that the SNP molecular marker developed in the application has a significant influence on four growth traits of the grass carp, including body length, body height, body width and body weight (P<0.05), and the allele A has more excellent growth traits. The application discloses application of the SNP molecular marker, amplification primers or kits in breeding of the grass carp with excellent growth traits. The SNP molecular marker provided in the application is not limited by the age and gender of the grass carp, and can be used for breeding of the grass carp with excellent growth traits, and even accurate screening can be performed just after hatching, so that the breeding process of the grass carp with excellent growth traits is greatly accelerated.

The invention relates to the technical field of molecular breeding, in particular to a novel rice Gn1a allele Gn1a G556D and application of the novel rice Gn1a allele Gn1a G556D in breeding. The invention provides a new allele Gn1a G556D of the rice Gn1a gene and a cytokinin oxidase / dehydrogenase OsCKX2 mutant encoded by the same, and the allele can improve the panicle type structure and increase the yield, can be used for improving germplasm resources of plants and cultivating new varieties of high-yield plants, and has important application value in the field of plant breeding.

The invention discloses a wheat haploid accurate identification and breeding method based on molecular markers, and belongs to the technical field of agricultural biology. The method comprises the following steps: hybridizing a created multifunctional haploid induction line HIP-Plus serving as a male parent with a target female parent, and saving immature embryos to obtain seedlings; a three-stage identification system comprising visual preliminary screening, molecular confirmation and early selection is adopted to carry out accurate screening on seedlings: firstly, preliminary screening is carried out based on embryo color, then haploid confirmation and transgenic component detection are carried out by utilizing TaMTL allele specific markers, and finally genetic typing is carried out through important character function markers to screen out elite haploids; and carrying out chromosome doubling to obtain a double haploid homozygous line. According to the method, the haploid identification accuracy is improved, the breeding period is shortened, the early directional selection of the target agronomic traits in the haploid stage is realized, and the breeding efficiency and accuracy are greatly improved.

The application belongs to the technical field of cotton molecular breeding, and particularly relates to a KASP molecular marker associated with the flowering trait of upland cotton and an application thereof. The nucleotide sequence of the molecular marker is shown as SEQ ID NO. 27. The molecular marker contains a SNP site. The SNP site is located at the 14th bp from the 5' end of the sequence shown as SEQ ID NO. 27. The polymorphism is G / A. The A allele is a favorable allele for promoting early flowering. The application also develops a primer set for detecting the molecular marker. The early flowering germplasm resource of upland cotton can be screened, the flowering time of upland cotton can be predicted, or early flowering varieties of upland cotton can be assistedly selected by using the primer set. The application also develops a method for assisting in the selection of early flowering varieties of upland cotton. The method can shorten the breeding cycle of the flowering trait of cotton and improve the breeding efficiency of the early flowering trait.

To provide new plants exhibiting improved resistance to nematodes, seeds and parts of the plants, methods of making and using such seeds and plants, and new SmD1 alleles that result in modified SmD1 proteins associated with such improved resistance to nematodes.SOLUTION: Provided are plants comprising SmD1 alleles encoding SmD1 proteins having at least 90% amino acid sequence identity to a particular sequence, wherein the SmD1 proteins comprise missense mutations resulting in modified SmD1 proteins that confer enhanced nematode resistance.SELECTED DRAWING: None

The invention provides a method for evaluating or predicting the chlorophyll content of sugarcane, which utilizes a molecular marker obviously associated with the chlorophyll content of sugarcane to accurately distinguish germplasm with high / low chlorophyll content. The method is suitable for sugarcane filial generation screening, core germplasm evaluation and gene localization, and provides a direct target for sugarcane photosynthetic efficiency improvement. The molecular marker is developed based on a GWAS method of Kmer, and the method avoids allele dose estimation deviation caused by traditional sugarcane high-power genetic complexity and can solve the problem that sugarcane correlation analysis is difficult.

The invention belongs to the technical field of plant bioengineering, and discloses a KASP primer group for detecting wheat plant height and yield synergistically improved QTL locus QPH-6A and application, the primer group is designed for SNP loci at the physical positions 11, 164 and 425 bp of wheat 6A chromosomes, and the primer group comprises two specific forward primers for different alleles and a universal reverse primer. By utilizing the primer group and the detection method provided by the invention, whether the wheat material carries the QPH-6A excellent allele or not can be quickly and accurately identified. The excellent allelic gene has a unique'dwarfing without yield reduction 'genetic effect, and the thousand seed weight, the ear length and the ear seed number can be remarkably increased while the plant height is reduced to a suitable lodging-resistant range (75-85 cm). According to the invention, the problems of too short plant, reduced biomass, premature senility and the like caused by the traditional dwarf gene in arid ecological regions such as Xinjiang are solved, and an efficient breeding tool is provided for cultivating a new high-yield ideal plant type wheat variety suitable for an arid environment.

Provided herein is the identification and markers and causal genes associated with day-neutral autoflowering in plants and their use in selecting plants, including Cannabis plants, having autoflowering activity. The markers are useful for breeding autoflowering plants by obtaining nucleic acids, detecting one or more markers that indicate autoflowering activity, and establishing plant lines having such characteristics. Also provided are methods of editing plants to establish plant lines having autoflowering allelic variations.

A wheat transgenic plant carrying restorer of fertility genes specific to T. timopheevii CMS cytoplasm.

Plants, cells, tissues, and germplasms comprising genes and labeled alleles associated with increased resistance to gray leaf spot are provided. Also provided are methods of breeding plants having alleles associated with increased resistance to gray leaf spot and methods of identifying and selecting plants having alleles associated with increased resistance to gray leaf spot. Methods for identifying novel genes encoding proteins that provide resistance to gray leaf spot in plants and uses thereof are provided. The genes and marker alleles of the present disclosure can be used to produce gray leaf spot resistant plants by breeding, transgenic modification or genome editing.

The invention discloses a chicken feed utilization rate character molecular detection kit matched data management system, particularly relates to the field of poultry genetic detection, and aims to solve the problem of feed utilization rate typing result deviation. The spatial drift and the time drift are synchronously stripped at a signal source; a curve generated after real-time correction is subjected to global screening through a machine learning model, abnormal signals are isolated immediately in high-throughput operation, and amplification features are strictly aligned with physical positions; a subsequent allele map and a feeding index are integrated at a time on a data management platform, and each feed conversion track has a unique and credible genetic fingerprint; the thermal light field model is self-learned along with batches, the drift compensation precision keeps convergence along with the detection quantity, and the drift compensation precision is not dragged by hidden deviation after long-term operation; a breeding window is compressed from a periodic level to near real-time, the feed utilization rate improvement rhythm is accelerated, and resource investment is more controllable.

A method identifies and selects plants resistant to a fungal disease comprises (a) extraction of nucleic acid from a plant; (b) analysis of extracted nucleic acid for the presence of markers associated with increased fungal resistance within a single chromosome interval; and (c) selection of the plants that have these markers. Furthermore, a method sufficient for introgression into plants of fungal disease resistance alleles comprises (a) crossing parents of plants identified by the first embodiment method with other parents that do not have this resistance; (b) select progenies possessing markers associated with increased resistance to fungal disease using the method as defined in the first achievement; and (c) backcross in one or more cycles the selected progenies with the recurrent genitor to develop new progenies.