194 results about "Allele" patented technology

The invention belongs to the technical field of aquatic animal molecular markers and aquatic genetic breeding, and particularly relates to an SNP (Single Nucleotide Polymorphism) molecular marker remarkably related to growth traits of grass carp and application of the SNP molecular marker. The SNP molecular marker is located at the position of 23608308bp of the eleventh chromosome (SLG1123608308) of the grass carp, and is Tgt; and A basic group mutation. Experiments show that the SNP molecular marker developed by the invention has significant influence on four growth traits (Plt; 0.05), and the allele A has more excellent growth traits. The invention discloses application of an SNP (Single Nucleotide Polymorphism) molecular marker, an amplification primer or a kit in breeding grass carp with excellent growth traits. The SNP molecular marker provided by the invention is not limited by the age, sex and the like of the grass carp, can be used for breeding grass carp varieties with excellent growth traits, and even can be accurately screened after being hatched, so that the breeding process of the grass carp varieties with excellent growth traits is greatly accelerated.

The present invention relates to tomato plants comprising two copies of an allele associated with enhanced resistance to ToBRFV infection. The invention also relates to seeds and parts of said plants, such as fruits. The invention further relates to methods of making, identifying and using such seeds and plants.

The invention discloses a phytolacca americana seed EMS mutation library establishment method, and belongs to the technical field of mutation library construction.The phytolacca americana seed EMS mutation library establishment method comprises the steps that concentrated sulfuric acid is used for pretreating phytolacca americana seeds, then germination accelerating is conducted on the seeds, and the seeds are treated with an EMS solution when the seeds are white, so that the problems that phytolacca americana seed coats are thick, and the sensitivity to EMS is low are solved; according to the mutagenesis method disclosed by the invention, high-density series allele point mutation can be induced and generated, the mutagenesis frequency is relatively high, and meanwhile, chromosome distortion is less caused. In the mutagenesis process, abundant genetic variation can be more efficiently created, and more possibilities are provided for screening mutants with excellent characters. Besides, the method has a remarkable advantage in the aspect of cost, the required chemical reagent is cheap, the experimental operation process is simple, and complicated equipment and technology are not required like common irradiation. The breeding cost is reduced, the working efficiency is improved, and wide popularization and application are facilitated.

The invention discloses an SNP (Single Nucleotide Polymorphism) marker for auxiliary selection of the leaf number of brassica napus, a primer group, a detection method and application, and relates to the technical field of biology and rape breeding. The SNP marker is located at the 9283404 bp position of a brassica napus A02 chromosome, a basic group of the site is a T allele or a C allele, and the T allele is associated with a leafy character. The SNP marker provided by the invention can solve the technical problem that the breeding efficiency of the high-photosynthetic-efficiency ideal-plant-type rape variety is restricted due to the fact that an effective molecular marker closely linked with the leaf number character of the brassica napus is lacked in the prior art and the character cannot be quickly and accurately screened in the early breeding stage.

The present invention relates to methods of breeding with spotted watermelon, Citrullus lanatus, lines and varieties. More specifically, methods of making homozygous-spotted diploid watermelon lines (HSDL) for use in breeding and methods of making homozygous-spotted tetraploid watermelon lines (HSDL) for use in breeding are described. Further, methods of producing 33%, 66%, or 100% spotted triploid hybrid watermelon plants, as well as methods of producing 50% or 100% spotted diploid hybrid watermelon plants, are described. This invention further relates to markers able to determine the percentage of spotted allele present in spotted watermelon lines and varieties. In addition, this invention relates to new and distinctive lines and varieties of watermelon with a spotted phenotype.

The invention relates to the technical field of molecular markers and animal genetic breeding, in particular to an SNP marker influencing pig UMP content and application thereof. Whole genome re-sequencing and GWAS analysis are adopted, American-series purebred landrace pigs, American-series purebred large white pigs and American-series purebred duroc are used as research objects, seven SNP markers related to the pig UMP content are researched and determined, by optimizing dominant alleles of the SNP, the frequency of the dominant alleles can be increased generation by generation, the UMP content can be increased, and the pig UMP content can be improved. The pig genetic improvement progress is accelerated, so that the economic benefit of breeding pig breeding is effectively improved.

The invention discloses an application of an MLANA gene SNP molecular marker in black-bone chicken molecular marker-assisted breeding, the SNP molecular marker is located at the 28230965th site on a Z chromosome and is T or C polymorphism, and a chicken to be bred with a CT dominant genotype is an individual with high huckle muscle black color. The MLANA gene SNP molecular marker is adopted in black-bone chicken muscle black color molecular marker-assisted breeding, a CT dominant allele type can be used as an important molecular marker for breeding a thigh chicken brightness L * value, and breeding for improving thigh muscle black color is assisted by a method of selecting and remaining a CT dominant genotype individual and eliminating other genotype individuals. And the progress of thigh muscle black color generation breeding is accelerated.

Provided is a gene ZmFIE2 for regulating maize kernel development, an encoded protein thereof, an InDel molecular marker, and a use thereof, aiming to solve the current technical problems of low maize haploid induction rate, long breeding cycle, and low maize yield. An InDel molecular marker for detecting ZmFIE2 mutants was screened out, and a primer for InDel molecular marker was designed, the InDel molecular marker and the primer thereof can be applied to the breeding of maize kernel size varieties and maize haploid or diploid lines. From the epigenetic level, it was found that ZmFIE2 regulates the development of maize embryo and endosperm, affects the accumulation of kernel storage materials, and affects the cell cycle process of endosperm cells, which has great value in improving maize yield. By using multiple created ZmFIE2 allele mutants, research has found that the loss of ZmFIE2 function can directly determine the division of endosperm cells and apomixis, which will provide new gene resources and new ways to achieve high-frequency haploid induction and heterosis fixation in maize.

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker related to the salt tolerance of island cotton and application of the SNP molecular marker. The site of the SNP molecular marker is located at the 45674375th site of a D02 chromosome of gossypium barbadense, and the allele at the 45674375th site is A or G. According to the invention, whole genome association analysis is carried out on the relative plant height and the relative overground part fresh weight in salt stress and salt-free stress environments, the SNP site related to the salt tolerance of the sea island cotton is obtained through screening, and the SNP molecular marker is accurate and efficient in detection and convenient and stable in amplification, can be used for molecular marker-assisted selection, and can be used for detecting the salt tolerance of the sea island cotton. The identification efficiency of different salt-tolerant cotton varieties is improved.

The invention provides an SNP (Single Nucleotide Polymorphism) molecular marker combination for predicting a Chinese rose flower type and application of the SNP molecular marker combination. The combination comprises 2001 SNP loci distributed on 14 chromosomes of the rosa chinensis, and can be used for predicting the flower types of the rosa chinensis, including circles, irregular circles and stars. The SNP loci are determined through genome-wide association analysis and screening of a plurality of software models, and have dominant alleles which are positively and remarkably associated with the Chinese rose types. The invention also relates to a prediction probe and a prediction chip based on the SNP molecular marker combination, and applications of the prediction probe and the prediction chip in China rose type prediction. According to the SNP molecular marker combination, the prediction probe or the prediction chip, the prediction model is combined, the florescence characteristics of the Chinese roses can be predicted 6-8 months after the Chinese roses are planted, the breeding period is greatly shortened to 2-3 years, meanwhile, the prediction precision is improved, the cost is reduced, the screening efficiency is improved, a variety right technical barrier is formed, and the SNP molecular marker combination, the prediction probe or the prediction chip have important theoretical value and application significance.

The invention belongs to the technical field of gene detection, and particularly discloses an STR fluorescent multiplex amplification detection kit for pigeon individual recognition, a detection method and application of the STR fluorescent multiplex amplification detection kit. The kit comprises 19 STR (short tandem repeat) loci and one sex identification locus of the domestic pigeon, also comprises an allele lader and the like, can more accurately judge the typing of the loci, has high cumulative paternity exclusion rate, can be applied to individual identification and paternity relationship identification of the domestic pigeon, has high detection sensitivity, and can obtain an accurate typing result only by 0.5 ng of DNA (deoxyribonucleic acid).

The invention belongs to the technical field of crop molecular markers, and particularly discloses a molecular marker for identifying the salt tolerance of brassica napus in the germination period and application of the molecular marker. The molecular marker is located at the position of an A02 chromosome scafoldA028707654 of a brassica napus reference genome ZS11.v10, and the allelotype of the molecular marker is wild type C or mutant type CATATT insertion. The invention also provides a specific primer group for detecting the molecular marker, and the salt tolerance (sensitive type or tolerance type) of the brassica napus line can be quickly identified according to the existence of 411bp and 416bp bands through double PCR amplification and electrophoresis detection. The molecular marker is significantly associated with salt tolerance phenotypes, the detection method is rapid, accurate and low in cost, the molecular marker can be used for brassica napus salt tolerance germplasm resource screening and molecular marker-assisted breeding, and the breeding efficiency is significantly improved.

The invention belongs to the technical field of molecular marker development and molecular marker assisted breeding, and particularly relates to a brassica napus salt tolerance related KASP molecular marker and application thereof. The invention discloses novel salt-tolerant allelic mutation of major QTL (quantitative trait loci) of A06 chromosome and C08 chromosome of brassica napus. A molecular marker is obtained through whole genome association analysis. According to the brassica napus salt tolerance related KASP molecular marker disclosed by the invention, the amplification effect of marking SNPA06.1, SNPA06.2 and SNPC08 is optimal, and the SNP variation of salt-tolerant germplasm and sensitive germplasm can be obviously distinguished.

The application belongs to the technical field of molecular breeding of Brassica napus, and particularly relates to a SNP molecular marker related to the thousand seed weight of Brassica napus and application thereof. The SNP molecular marker is located at the position of 43,480,336 of the scaffold C05 chromosome of Brassica napus reference genome ZS11 v0. The site has T / G polymorphism, wherein the T allele is a favorable allele variation for increasing the thousand seed weight of Brassica napus. The SNP molecular marker can be used for screening high thousand seed weight potential materials at the seedling stage or early stage through DNA detection, improving the high yield breeding efficiency of Brassica napus, and can be used for screening high thousand seed weight materials of Brassica napus, molecular assisted selection breeding, evaluation of germplasm resources and improvement of yield-related traits.

IL33 antagonists, alone or in combination with IL-4R antagonists, can be used to treat or inhibit eosinophilic asthma, eosinophilic COPD, eosinophilic ACOS, and nasal polyps in subjects having one or more risk alleles in intronic IL1RL1 variant rs1420101, in IL33 variant rs1342326, in both, or in variants in linkage disequilibrium therewith.

The invention relates to the technical field of biomolecular markers, and provides a chicken butyric acid level and / or death rate related SNP marker and application thereof, the SNP marker comprises a 106476774th nucleotide site corresponding to a No.3 chromosome of a GRCg6a-galGal6 genome, and the basic group of the nucleotide site is A or T. The invention also provides an application of the SNP marker in chicken butyric acid level and / or death rate related SNP marker in chicken butyric acid level and / or death rate related SNP marker in chicken butyric acid level and / or death rate related SNP marker in chicken butyric acid level and / or death rate related SNP marker. By optimizing the dominant alleles of the SNP, broilers with the butyric acid level and / or high and low mortality can be rapidly identified, broilers with high mortality can be eliminated, the frequency of the dominant alleles is increased generation by generation through breeding, the butyric acid level and / or mortality of the broilers, especially the 28-42-day-old stage, of the broilers is reduced, the genetic improvement progress of the domestic broilers is accelerated, and the breeding efficiency of the domestic broilers is improved. Therefore, the economic benefit of chicken breeding is effectively improved, and the application value is high.

Provided are a rice mutant gene and the use thereof in regulating the plant height of rice. The rice mutant gene at least has an allele mutation, and the allele mutation is the mutation into Asn from Asp at the 377th site in an amino acid sequence shown as SEQ ID No. 26. The mutant gene can effectively regulate and control the rice plant height, and has good application potential in plant architecture breeding of rice varieties.

The invention discloses molecular marker identification and application of dwarf round-grain wheat alleles. The invention belongs to the field of plant breeding, and particularly relates to molecular marker identification and application of dwarf round-grain wheat alleles. The method for identifying or assisting in identifying the wheat dwarf round grains comprises the steps that the genotype of an SNP site in a wheat genome to be detected is detected, the phenotype of the wheat dwarf round grains is identified or assisting in identifying according to the genotype, the SNP site is an SNP site on a wheat 3B chromosome, the nucleotide type of the SNP site is T or C, and the SNP site is the 185th nucleotide of a sequence 1 in a sequence table.

The invention discloses an application of an MITF gene SNP molecular marker in black-bone chicken muscle and skin black color molecular marker assisted breeding. The SNP molecular marker comprises the following two SNP sites: an SNP1 molecular marker is located at the 15747890th site of a 12 # chromosome positive-sense strand, is in a C or T polymorphism, is numbered as rs316573471, and has a dominant genotype of TT; the SNP2 molecular marker is located at the 15751940th site of a positive-sense strand of a chromosome 12, is A or G polymorphism, and is numbered as rs14045480, and the dominant genotype is AA. By adopting the MITF gene SNP molecular marker in black-bone chicken muscle black color molecular marker assisted breeding, TT and AA dominant allelotype can be respectively used as important molecular markers for breeding thigh muscle brightness L * value and back skin brightness L * value, and TT genotype and AA genotype individuals are selected and reserved; the method of eliminating other genotype individuals is used for assisting in improving the breeding of the huckle muscle black color and the back skin black color, and the progress of the generation breeding of the huckle muscle and the back skin black color is accelerated.

The invention relates to a gene for regulating and responding to environment temperature change and application thereof. Specifically, the invention provides the application of the TOMS1 (Thomson MeSocotyl1) or the allele of the TOMS1 in the aspect of regulating and controlling the rice to respond to the environment temperature change so as to improve the yield. The invention further discloses a method for cultivating the plants suitable for growing at the high environment temperature. The TOMS1 gene has a wide application prospect in high environment temperature adaptation and high-yield plant breeding.

The present application relates to the field of molecular markers and animal genetic breeding technology, and particularly relates to a SNP marker affecting pig purine base content and application thereof. Based on three test pig populations of American purebred Landrace, American purebred Large White and American purebred Duroc, the present application uses pig whole genome resequencing and GWAS analysis to research and determine 15 SNP markers significantly affecting pig purine base content. By selecting the advantageous alleles of the above SNP markers, the frequency of the advantageous alleles can be increased generation by generation, the corresponding purine content can be reduced or increased, the progress of pig genetic improvement can be accelerated, and the economic benefits of pig breeding can be effectively improved.

A method of enhancing grain yield of a Gramineae plant is provided. The method comprises:(a) crossing a recipient plant with a donor plant comprising a sequence variation in a TPR allele, said sequence variation imparting enhanced grain yield; and(b) selecting a progeny plant of said crossing comprising said sequence variation by identifying said variation, said progeny plant being characterized by an improved grain yield relative to said recipient plant. Also provided are plants and processed products resultant of the method.

The invention relates to methods of identifying and characterizing a Cannabis spp. plant with respect to a flower to leaf ratio trait comprising genotyping the plant for a quantitative trait locus (QTL) associated with a flower to leaf ratio trait, and to methods of producing plants having a flower to leaf ratio trait of interest based on defined allelic states of polymorphisms defining the QTL. Also provided are Cannabis spp. plants having a flower to leaf ratio trait of interest comprising defined allelic states of polymorphisms defining the QTL and plants identified, characterized or produced by the methods described herein. The invention further relates to marker assisted selection and marker assisted breeding methods, in particular using a combination of specific markers provided, for obtaining plants having a flower to leaf ratio trait of interest or for modulating the flower to leaf ratio of cannabis plants.

The application belongs to the technical field of aquatic animal molecular marker and aquatic genetic breeding, and particularly relates to a SNP molecular marker significantly related to growth traits of grass carp and application thereof. The SNP molecular marker is located at 23608308 bp of the 11th chromosome of the grass carp (SLG11_23608308), and is a T>A base mutation. Experiments show that the SNP molecular marker developed in the application has a significant influence on four growth traits of the grass carp, including body length, body height, body width and body weight (P<0.05), and the allele A has more excellent growth traits. The application discloses application of the SNP molecular marker, amplification primers or kits in breeding of the grass carp with excellent growth traits. The SNP molecular marker provided in the application is not limited by the age and gender of the grass carp, and can be used for breeding of the grass carp with excellent growth traits, and even accurate screening can be performed just after hatching, so that the breeding process of the grass carp with excellent growth traits is greatly accelerated.

The invention relates to the technical field of molecular breeding, in particular to a novel rice Gn1a allele Gn1a G556D and application of the novel rice Gn1a allele Gn1a G556D in breeding. The invention provides a new allele Gn1a G556D of the rice Gn1a gene and a cytokinin oxidase / dehydrogenase OsCKX2 mutant encoded by the same, and the allele can improve the panicle type structure and increase the yield, can be used for improving germplasm resources of plants and cultivating new varieties of high-yield plants, and has important application value in the field of plant breeding.

The present invention relates to a QTL allele in maize associated with drought resistance and carbon isotope composition as well as specific marker alleles associated with the QTL allele. The present invention further relates methods for identifying maize plants based on screening for the presence of the QTL allele or marker alleles. The invention also relates to methods for modifying drought resistance and carbon isotope composition in maize plants.

The invention discloses a wheat haploid accurate identification and breeding method based on molecular markers, and belongs to the technical field of agricultural biology. The method comprises the following steps: hybridizing a created multifunctional haploid induction line HIP-Plus serving as a male parent with a target female parent, and saving immature embryos to obtain seedlings; a three-stage identification system comprising visual preliminary screening, molecular confirmation and early selection is adopted to carry out accurate screening on seedlings: firstly, preliminary screening is carried out based on embryo color, then haploid confirmation and transgenic component detection are carried out by utilizing TaMTL allele specific markers, and finally genetic typing is carried out through important character function markers to screen out elite haploids; and carrying out chromosome doubling to obtain a double haploid homozygous line. According to the method, the haploid identification accuracy is improved, the breeding period is shortened, the early directional selection of the target agronomic traits in the haploid stage is realized, and the breeding efficiency and accuracy are greatly improved.

The invention relates to the technical field of plant gene editing, in particular to a method for converting plant alleles among chromosomes by utilizing CRISPR / Cas9 (clustered regularly interspaced short palindromic repeats / CRISPR associated protein 9) and super recombinant lines (HRLs), and the conversion efficiency of the alleles is remarkably improved by combined use of the CRISPR / Cas9 and the super recombinant lines (HRLs). In the allele conversion process, the male parent allelic variation is repaired and converted by taking the excellent allele in the female parent plant as a template, so that the problem that the bad gene closely linked with the target gene is introduced together is avoided, and the quality and performance of the new variety are improved. According to the allele transformation method, the multi-generation backcross and screening process is not needed, the breeding period is greatly shortened, and the breeding cost is reduced. The CRISPR / Cas9 system can accurately identify and cut a specific DNA sequence, and realizes accurate conversion between homologous chromosomes of alleles in combination with an efficient homologous recombination repair mechanism of a super-recombinant line (HRLs).

The embodiment of the invention discloses a breeding method of a novel rice blast-resistant hybrid japonica rice combination. According to the method, different rice blast genes are directionally introduced into different parents of the hybrid japonica rice, so that excellent rice blast resistant maintainer line, sterile line and restorer line materials can be obtained, meanwhile, pi2 and pigm are respectively introduced into male and female parents, and the rice blast resistant genes such as pi2 and pigm on the same chromosome can be polymerized into the same hybrid japonica rice variety; the rice blast resistance of the hybrid combination is improved, the adaptability of the new combination is improved, and the breeding method has important significance on the rice blast resistance breeding work of the hybrid japonica rice.