1162 results about "Multiplex" patented technology

The present invention, among other things, provides technologies for detecting and / or quantifying nucleic acids in cells, tissues, organs or organisms. In some embodiments, through sequential barcoding, the present invention provides methods for high-throughput profiling of a large number of targets, such as transcripts and / or DNA loci.

Provided herein are methods of performing multiplex detection of ligand binding kinetics. In some embodiments, the methods include contacting ligands with an array of nucleic acid barcoded oscillators disposed on a first surface of a substrate that comprises an electrically conductive coating, applying an AC electric field to the substrate sufficient to induce the nucleic acid barcoded oscillators to oscillate proximal to the first surface of the substrate, and detecting changes in oscillation amplitudes of the nucleic acid barcoded oscillators over a duration to produce sets of ligand binding data. In some embodiments, the methods also include contacting barcode decoding nucleic acids with the array of nucleic acid barcoded oscillators applying an AC electric field to the substrate sufficient to induce the nucleic acid barcoded oscillators to oscillate proximal to the first surface of the substrate, and detecting changes in oscillation amplitudes of the nucleic acid barcoded oscillators over a duration to produce sets of barcode decoding data.

The invention discloses SSR (simple sequence repeat) multiple PCR (polymerase chain reaction) primers for paternity test of grass carp and application of the SSR multiple PCR primers. The primers comprise 16 pairs of specific primers, and base sequences of the 16 pairs of specific primers are respectively shown as SEQ ID NO.1-32 in sequence. The invention further discloses a fish paternity test kit comprising the SSR multiple PCR primer, an SSR multiple fluorescent PCR method for grass carp paternity test and application of the SSR multiple PCR primer, the kit or the method in grass carp paternity test. According to the kit, 16 pairs of specific SSR primers, multiple fluorescent PCR and a universal amplification primer technology are combined, 16 sites can be detected at a time, universal primers can be repeatedly used, and compared with simple single-site detection, the efficiency is improved, and the cost is reduced; the microsatellite loci contained in the invention are 3-6 basic groups, the allele size interpretation is more accurate, and the accuracy of genotype data is improved.

The invention relates to the field of biological detection, in particular to a mini STR (short tandem repeat) double-multiplex amplification system and kit for degrading 25 gene loci of a sample. The invention provides a kit. The kit comprises 25 gene loci; the 25 gene loci comprise 23 autosomal STR gene loci, one sex gene locus and one Yindel gene locus. According to the kit provided by the invention, the primers are divided into a group A and a group B, and all gene loci are miniS TR gene loci on the basis of using current mainstream instruments and equipment. The two tubes of primers use the same experiment condition, and amplification and electrophoresis can be carried out at the same time. Compared with the existing miniSTR kit, the kit disclosed by the invention can be independently amplified and does not need to be combined with other autosomal STR kits for use, and the typing of 25 gene loci can be obtained through one-time detection. The effective detection rate of degraded samples is greatly improved, and technical support is provided for detection of difficult cases.

The invention provides a kit and a method for detecting common fusion genes of lymphoid leukemia based on a multiple digital PCR (Polymerase Chain Reaction) method. By constructing a synergistic effect mechanism of the primer and the fluorescent probe and optimizing matching of the primer, the probe and a fluorescent marker, non-specific binding among amplification products is effectively avoided. A composite reaction aid with a specific formula, namely a PCR enhancer, is innovatively introduced, so that the amplification efficiency and specificity of a multiple detection system are remarkably improved. According to the kit provided by the invention, the integrated detection capability on seven fluorescent channels, namely Atto 425, VIC, FAM, ROX, CY5, CY5.5 and CY7, is realized in a single-hole design. By innovatively constructing an ROX + CY5 and Atto425 + VIC dual-channel joint detection system, when the ROX and CY5 channels or Atto425 and VIC channels in the same detection hole simultaneously present positive signals, two additional detection targets can be specifically interpreted. The innovative detection strategy based on channel combination enables the kit to realize precise detection of 18 fusion genes only through two detection holes in a breakthrough manner.

The invention relates to the technical field of biology, in particular to an anti-pollution freeze-dried eight-color Mini-STR composite amplification kit. Through design and optimization, the primer group, the reagent and the kit for detecting the eight-color multiple fluorescence labeling Mini-STR capable of identifying 25 STR gene loci are developed; the primer group, the reagent and the kit are used for detecting forensic samples, the detection sites are more, the detection accuracy is higher, the anti-pollution capacity is high, the detection capacity on trace samples is higher, the detection rate of degraded sample DNA can be remarkably increased, and the kit is higher in stability and has wide application value.

The present disclosure provides methods for conducting in situ multiplex and multi-omics detection and identification using coded padlocks probes. The methods comprise simultaneous use of RNA-specific padlock probes and polypeptide-specific padlock probes to detect both RNA and polypeptides in a cellular sample. Both types of probes include a barcode that unique identifies the RNA or polypeptide that that padlock probe detects. Both types of probes also include a batch-specific sequencing primer binding site to enable sequencing a desired subset of concatemer template molecules. Use of the batch-specific sequencing primers reduces overcrowding signals and images, to produces optical images that are intense and resolvable. By conducting multiple rounds of sequencing on the same cellular sample using different batch-specific sequencing primers enables multiplex and multi-omics sequencing to reveal numerous target RNAs and their encoded polypeptides.

The invention belongs to the field of biological detection, and particularly relates to an ewing sarcoma detection primer group, a kit and application thereof. The Ewing sarcoma detection primer group comprises a primer group A and a primer group B, and totally has 17 primer sequences. The primer group is designed, Ewing sarcoma characteristic variation genes are detected through multiple fluorescent PCR and a high-resolution capillary electrophoresis apparatus, 24 variation types fused by 7 types of genes can be detected at a time, the lowest detection limit is 10 copies, and the shortest detection time is 240 minutes. The detection result of the primer group disclosed by the invention can pass Sanger sequencing verification and is completely consistent with the morphological diagnosis result, the methodological consistency reaches 100%, and a sensitive and efficient Ewing sarcoma genetic variation detection method is provided.

Provided herein are methods, systems, compositions, and kits for detecting and decoding an encoded assay. The present disclosure provides low cost, multiplexed and automatable assays by hypercoding, a scalable technology for detection and quantitation of multi-omics targets. Hypercoding, or coding, utilizes signals from fluorescent hybridization with an error-corrected code to enable accurate detection and high-plexity targets of interest from samples. The present disclosure provides encoded assays for multiplex target detection from a sample suitable for detection by hybridization. Detection polynucleotide complexes, or detection oligonucleotides and anchor oligonucleotides, of the present disclosure are utilized in the detection and decoding of the hypercodes to produce optical signals capable of being decoded by a soft decision decoding algorithm for determining the presence of a target of interest, or multiple targets of interest, in a sample.

The invention provides a method for simultaneously detecting three hematogenous parasites based on RAA-LFS and application of the method. The method comprises the following steps: firstly, screening target genes of genomic DNA of specific amplified toxoplasma gondii, babesia and plasmodium and primers of the target genes; a primer and probe combination for specifically amplifying the 18s rRNA-1 gene of the toxoplasma gondii, the 18s rRNA-2 gene of the babesia and the 18s rRNA-3 gene of the plasmodium is determined; rapid multiple detection is completed in a reaction system by utilizing an RAA constant-temperature amplification technology, and a detection result is intuitively judged by combining a lateral flow chromatography test strip, so that the detection efficiency is greatly improved, and the result can be obtained in a short time. The RAA-LFS detection method provided by the invention has remarkable advantages in application scenes such as epidemic disease prevention and control or blood screening.

The present invention provides multiplex assays for improved scoring of tumour tissues stained with an immune checkpoint protein such as VISTA. Methods of ranking multiple tumour tissues and of diagnosing cancer patients are also provided. The methods disclosed are also useful for identifying patient responsive to anticancer therapy.

Disclosed is a multiplex quantum dot-based quantitative test card, and a preparation method, apparatus, and use method thereof in the technical field of biological testing, the multiplex quantum dot-based quantitative test card including a multiplex card housing and a test strip, where the multiplex card housing is provided with a plurality of reaction slots configured to accommodate the test strip; the multiplex card housing further includes a plurality of sample loading ports and a plurality of observation windows, where the sample loading ports and the observation windows are in one-to-one correspondence with the reaction slots; and the test strip includes a conjugate pad coated with quantum dot-labeled antibodies, where the quantum dot-labeled antibodies serve as chromogenic substances.

The invention relates to the technical field of life science, in particular to a method for constructing an atrophic gastritis-based SPEM lesion model, which comprises the following steps: S1, constructing an Slc7a11 conditional knockout mouse strain: carrying out cage hybridization on an Slc7a11 flox / flox mouse and an ATP4b-Cre transgenic mouse, and knocking out a gastric wall cell specific Slc7a11 gene; s2, carrying out genotyping identification; the identification method based on the atrophic gastritis SPEM lesion model and the construction method based on the atrophic gastritis SPEM lesion model comprise the following steps: S1, pathological model verification: taking a mouse gastric mucosa tissue slice, quantitatively analyzing the proportion of an SPEM region by adopting an immunofluorescence double-standard technology, and confirming Slc7a11 knockout induced SPEM pathological characteristics in combination with the existence of SPEM in the gastric mucosa; and S2, long-term pathology evolution monitoring. Through gastric wall cell specific Slc7a11 gene knockout and multiple PCR identification technologies, the initial response SPEM state after gastric mucosa damage is successfully simulated, and the kit is used for researching atrophic gastritis pathological mechanisms and evaluating drug intervention.

The invention designs a carbapenem drug-resistant gene nucleic acid fluorescent PCR method detection kit and application thereof, the kit uses Taqman fluorescent probe multiple amplification technology to detect carbapenem drug-resistant genes, the kit comprises a nucleic acid reaction liquid Mix1 and a nucleic acid reaction liquid Mix2, the nucleic acid reaction liquid Mix1 is used for detecting drug-resistant genes IMP, NDM and OXA48, the nucleic acid reaction liquid Mix2 is used for detecting drug-resistant genes IMP, NDM and OXA48, and the nucleic acid reaction liquid Mix2 is used for detecting drug-resistant genes IMP, NDM and OXA48. The nucleic acid reaction liquid Mix2 is used for detecting drug resistance genes VIM, KPC and OXA23. The method is high in detection specificity, and the detection result is visual and easy to interpret. Clinical doctors are assisted to judge respiratory tract pathogen infection more comprehensively and more accurately. The kit covers six carbapenem drug resistance genes, is more comprehensive and accurate in detection, and is high in specificity and sensitivity. Meanwhile, multiple sample types can be detected, a sputum sample, an excrement sample and a rectum swab sample are detected through paramagnetic particle extraction, and pure bacterial colonies are directly detected without extraction.

According to the nucleic acid multiple detection micro-fluidic chip and the detection method thereof, an exhaust channel and a waterproof breathable film are arranged in a reaction cavity and a detection cavity respectively, a valve block is additionally arranged between the reaction cavity and the detection cavity, the temperature rise phase change performance of the valve block and the characteristics of the waterproof breathable film are utilized, and the atmospheric pressure internal and external balance principle is combined, so that the nucleic acid multiple detection micro-fluidic chip is obtained. The controllable transfer of a sample to be detected in different chambers is ingeniously realized, and the sample backflow can be effectively prevented; the position relation and the shape of each flow channel between the reaction cavity and the detection cavity are designed and improved, and a simple pressure applying device is additionally arranged, so that a sample to be detected smoothly enters or flows out of a corresponding cavity and is stably retained and reacted in the cavity. The micro-fluidic chip is simple in structure, small in size and convenient to operate, adapts to more simple detection environments, really realizes low-cost integration and is suitable for large-scale production and application.

The invention relates to the technical field of molecular biological detection, in particular to a primer, a probe group and a kit for multiplex nucleic acid mass spectrometry detection of porcine diarrhea pathogens and application. By adopting a matrix-assisted laser desorption / ionization time-of-flight nucleic acid mass spectrometry technology, eight main pathogens such as PEDV, TGEV, PDCoV, PoRV, SADS-CoV, PBoV, HEV and salmonella can be rapidly, sensitively and specifically detected at the same time. By designing the specific primer and the unexpanded probe and combining multiple PCR amplification and mass spectrometry, the method realizes high-throughput and multi-pathogen simultaneous detection. Experimental results show that the method has high sensitivity and high specificity, has no false positive reaction, and shows 100% repeatability in 60 repeated experiments. Compared with a qPCR method, the detection consistency is as high as 96.2%. The method has the advantages of being easy and convenient to operate, high in detection speed and high in adaptability, can be widely applied to efficient monitoring of porcine diarrhea pathogens, and provides important technical support for early diagnosis, prevention and control of porcine diseases.

The invention provides a non-invasive fetal trisomy 21 syndrome prenatal screening kit based on digital PCR, and relates to the technical field of fetal trisomy 21 syndrome screening, according to a method for enriching fetal cfDNA in pregnant woman plasma, SPRIselect magnetic beads with low enrichment multiple and silicon hydroxyl magnetic beads are combined for use, and under a certain dosage, the recovery rate of fetal cfDNA short fragments reaches 95%; according to the provided multiple system construction method, positive gDNA and negative gDNA are obtained on the basis of a 21 trisomy positive cell line and a 21 trisomy negative cell line, low-proportion reference substances are prepared and used, internal reference sites and detection sites which are high in detection capacity and stable in detection performance are screened from the beginning, and compared with existing multiple system establishment and verification through direct use of pregnant woman plasma samples, the multiple system construction method has the advantages that the detection efficiency is high, and the detection cost is low. The to-be-detected sample with lower fetal cfDNA content can be stably detected.

Techniques are disclosed herein that encompass image pre-processing and a semi-supervised clustering for optimization and analysis of immune-enriched single-cell proteomics data generated via multiplexed imaging technologies. This is achieved through an image pre-processing pipeline, which converts image data contained in one type of file (e.g., .mcd) into another type of file (e.g., .tiff) and removes artifact signals from the image data using various algorithms to generate improved image data. Thereafter, a semi-supervised clustering pipeline analyzes the improved image data using various techniques, including implementing a supervised algorithm to identify metaclusters such as general immune phenotypes (e.g., CD4−T-cells, Macrophages, Neutrophils, etc.) as well as non-immune phenotypes while implementing an unsupervised algorithm that enables the identification of specific subclusters and a more in-depth cellular status characterization.

The present invention relates to a multiplex immunoassay method for diagnosing autoimmune nodopathy and, more specifically, to a multiplex immunoassay method, comprising the steps of: (1) binding CNTN1, Caspr1, NF155, and NF186 proteins each to microspheres having different color codes; (2) mixing the microspheres having different color codes to which the proteins are each bound; (3) treating the mixed microspheres with a biological sample to induce an antigen-antibody reaction; (4) adding a detection antibody; and (5) reading fluorescence information of the microspheres to measure the presence or absence of anti-CNTN1 antibody, anti-Caspr1 antibody, anti-NF155 antibody, or anti-NF186 antibody in the biological sample and quantify amounts thereof, thereby enabling simultaneous testing of four antibodies for rapid diagnosis of autoimmune nodopathy.

The invention discloses a multiplex fluorescent quantitative PCR (polymerase chain reaction) detection kit for prawn culture pathogenic bacteria, which is characterized in that the kit contains primers and probes for detecting the prawn culture pathogenic bacteria, wherein the primers and the probes are respectively used for detecting TDH-related hemolsin genes of vibrio parahaemolyticus, ORF107 genes of white spot syndrome viruses, small subunit ribosomal genes of shrimp enterocytozoon hepatopenaei, 37 kDa coat protein genes of infectious subcutaneous and hematopoietic necrosis viruses, DNA (deoxyribonucleic acid) primer genes of full-eye iridovirus 1 and PirA [pi] genes of pathogens of bacterial prawns acute hepatopancreas necrosis; the method has the advantage that the detection of eight prawn culture pathogens can be realized at the same time. The method comprises the following steps of: obtaining a tcdB gene of a high-pathogenicity vibrio and a hemolsin gene of a vibrio harveyi;

The invention can be used to provide a more efficient and less error-prone method of detecting variants in DNA, such as SNPs and indels. The invention also provides a method for performing inexpensive multiplex assays.

The present disclosure relates to proteomics, including the detection of immune cell target proteins as examples that the disclosed methods can be used for protein detection. Methods, compositions, and systems are described for identifying the presence of proteins, including detecting immune cell proteins from a sample using a combination of multiplex immuno-PCR with antibodies conjugated to oligonucleotides comprising an antibody-specific barcode flanked by PCR priming sites; detection of barcoded amplicons obtained from immuno-PCR with encoded padlock probes comprising codes specific for the barcodes / target protein; rolling circle amplification (RCA) of the circularized padlock probes; and determination of the sequence of the codes amplified by RCA by next generation sequencing or using fluorescently labeled hybridization probes.

The present disclosure provides peptides, chimeric molecular constructs, and compositions thereof, that can bind volatile organic compounds (VOCs) and be utilized to distinguish VOC profiles, e.g., indicative of disease, as well as related methods. A VOC-based gas sensing approach described in the present specification can be an effective screening tool, due, at least in part, to its speed, ease of use, sensitivity, specificity, and because it does not rely on binding to specific nucleic acid fragments or proteins to function.

This invention discloses primer compositions, detection products, and applications for detecting 17 pathogens, relating to the field of biotechnology. A primer composition for detecting 17 pathogens includes 17 pairs of amplification primers and 17 extension probes, as shown in SEQ ID NO: 1-51, respectively. This invention also provides detection products comprising the above primer compositions and their application in simultaneously detecting 17 pathogens for non-disease diagnostic purposes. The beneficial effects of this invention are that it designs 17 primer sets to simultaneously amplify DNA fragments containing specific target sequences of 17 pathogens in multiplex PCR, and performs single-base extension of these products using highly efficient single-base primers. Simultaneous detection and identification of 17 pathogens can be achieved using MALDI-TOF MS mass spectrometry, with accurate and highly specific detection results.

The invention relates to a primer composition for detecting genital tract infection related pathogens and drug-resistant genes thereof and application of the primer composition, and detection targets of the primer composition cover 69 genital tract infection related pathogens including HPV virus, candida disease, trichomoniasis, sexually transmitted diseases and the like. And an antibiotic strong associated drug resistance site for neisseria gonorrhoeae and mycoplasma genitalium. The primers in the primer composition not only can be independently used for PCR amplification, but also can be used for multiple PCR amplification in the same detection system, mutual interference among the primers is little, no obvious non-specific amplification exists, one-tube multiple PCR amplification reaction is efficiently realized under the condition that the number of the primers is up to 150, and the detection efficiency is high. Comprehensive screening of pathogens related to complex genital tract infection and drug resistance detection of neisseria gonorrhoeae and mycoplasma genitalium can be achieved through one-time detection, and the method has the advantages of being good in specificity, high in sensitivity and high in accuracy.

The invention relates to a data analysis method and system for multiple PCR multi-target parallel amplification, and relates to the field of biological detection.User features and respiratory tract features of a target user are collected and analyzed to obtain feature confidence, the features are utilized to predict multiple PCR detection results, error calculation is carried out on the results and an actual detection spectrum, and the accuracy of the results is improved. According to the multi-PCR multi-target parallel amplification data analysis method and system, the abnormal modes are detected in a classified mode in combination with the feature confidence coefficient, the actual detection spectrum is marked, the technical problems that multi-PCR multi-target parallel amplification data analysis is complex, errors are prone to occurring, and automatic and accurate analysis is difficult are solved, automatic and precise analysis of the multi-PCR multi-target parallel amplification data is achieved, the accuracy and efficiency of data analysis are effectively improved, and the data analysis efficiency is improved. Detection abnormity can be found in time, and the reliability and accuracy of clinical research are improved.

The invention belongs to the technical field of gene detection, and particularly relates to application of a CFTR gene mutation combination, an amplification reagent and a product in preparation of a CF risk assessment product. The CFTR gene mutation combination consists of an intron region mutation combination and an exon region mutation combination, based on the CFTR gene mutation combination, the invention further develops an amplification reagent and a CFTR gene mutation detection product, the amplification reagent and the CFTR gene mutation detection product comprise a primer combination for multiplex PCR amplification of the CFTR gene mutation combination, and the primer combination has the characteristics of high accuracy, high specificity and high sensitivity, can accurately detect related gene mutation, and provides a reliable basis for risk assessment and diagnosis of CF. When the amplification reagent and the CFTR gene mutation detection product are combined with sweat chlorine detection for use, the diagnosis rate of CF can be remarkably improved, and the amplification reagent has important application value in clinical diagnosis of CF and is expected to provide more powerful support for early diagnosis and treatment of CF patients.

The invention discloses a high-throughput composite STR (short tandem repeat) detection system based on next-generation sequencing. The high-throughput composite STR detection system comprises specific primer sequences designed for 291 STR loci; the 291 STR loci are composed of 106 autosome STR loci, one sex identification locus Amelogenin, 148 Y chromosome STR loci and 36 X chromosome STR loci, and the 291 STR loci are composed of 106 autosome STR loci, one sex identification locus Amelogenin, 148 Y chromosome STR loci and 36 X chromosome STR loci; the system is used for performing STR typing and is prepared into a kit for individual recognition, paternity identification and complex sample analysis. The STR detection system covers STR locus combinations of whole chromosomes (autosomes and X / Y chromosomes), breaks through the split design in the prior art, can overcome the limitation of the traditional capillary electrophoresis (CE) technology on complex samples, degraded samples and large-scale detection, improves the detection sensitivity, flux and accuracy, and has a good application prospect. And the requirements of identity identification of complex genetic relationships, mixed samples and disastrous events in judicial expertise are met.

The invention discloses a reagent and a method for detecting low-frequency pancreatic cancer KRAS G12D gene mutation based on a Cas12a-Cas13a combined reaction system. The reagent comprises a single-stranded substrate DNA (Deoxyribonucleic Acid), a TRNA (Transcription Ribonucleic Acid), a 12CrRNA, a 13CrRNA, a 12Report and a 13Report; the single-stranded substrate DNA is derived from genome DNA of a to-be-detected sample, after the genome DNA of the to-be-detected sample is extracted, asymmetric PCR is carried out by utilizing a PCR primer pair, namely, the single-stranded substrate DNA is amplified and comprises wild type WT and mutant type MT; the mutant MT is completely matched with a recognition region of 12CrRNA, and a base mismatch exists between the mutant MT and a recognition region of 13CrRNA; a base mismatch exists between the wild type WT and a recognition region of 12CrRNA, and the wild type WT is completely matched with a recognition region of 13CrRNA; the TRNA is a pre-synthesized single-stranded RNA, has a base mismatch with a recognition region of the 12CrRNA, and is completely matched with a recognition region of the 13CrRNA; the 12Reporter is a DNA (Deoxyribose Nucleic Acid) probe of the FAM and BHQ double-standard genes, and the 13Reporter is an RNA (Ribonucleic Acid) probe of the FAM and BHQ double-standard genes. The nucleic acid cleavage activities of Cas12a and Cas13a are jointly utilized to realize multiple signal amplification, so that low-frequency detection of KRAS G12D mutation, which is simple to operate and low in cost, is realized.

This invention provides a primer design method for multiplex PCR targeted sequencing technology. This method combines multiple indicators to carefully screen high-quality species genomes to design primers with high specificity and broad genome coverage. This method effectively optimizes the dimer formation of the primer system, improving the specificity and efficiency of detection. While ensuring high specificity, this technology significantly improves the performance of the multiplex primer system, optimizes the common dimer problem, and ensures the high efficiency of multiple pathogen detection.