1621 results about "Multiplex" patented technology

The invention is directed to methods and systems for carrying out one or more highly multiplexed cellular assays. In some embodiments, one or more channels of a fluidic device are provided with photopolymerizable polymer precursors and cells randomly disposed on a surface, after which positions of cells are measured by a detector, hydrogel chambers are synthesized by photopolymerization to enclose individual cells, and channels are loaded with assay reagents. Assay signals indicative of desired cellular characteristics can be generated for each of the enclosed cells.

Disclosed are a primer probe for PCR detection, a primer probe set, and an application thereof. The primer probe and the primer probe set can simultaneously perform analysis in two dimensions of a fluorescent channel and a melting temperature in a single tube reaction. That is, by using a same fluorescent channel, different targets may be detected by means of melting temperature characteristics; or when different fluorescent channels are used, a target type detection of a product of the number of fluorescent channels and the melting temperature characteristic may be achieved. Moreover, a method for performing multiplex PCR detection by using the primer probe has the advantages of being low in fluorescence background, adjustable in melting temperature, good in inclusiveness, and low in cost, achieving single tube reaction, and being simple and convenient to operate, not easy to cause pollution, high in sensitivity, and wide in applicable range.

The present invention, among other things, provides technologies for detecting and / or quantifying nucleic acids in cells, tissues, organs or organisms. In some embodiments, through sequential barcoding, the present invention provides methods for high-throughput profiling of a large number of targets, such as transcripts and / or DNA loci.

The invention discloses a multiple primer group for detecting pathogens of reproductive system diseases and a kit thereof. The kit comprises a combination of amplification primers and fluorescent probes of 10 pathogens, the pathogens comprise molluscum contagiosum virus, Snethia bacteria, gardnerella vaginalis, prevotella genus, campylobacter genus, giant globules genus, Atortobacter vaginalis, lactobacillus jensenii, lactobacillus crispatus and inert lactobacillus. The kit contains UDG enzyme and is used for preventing pollution of a PCR amplification product, and the accuracy of a detection result is ensured. The kit provided by the invention contains an internal reference control, can display the occurrence of false negative, and is used for monitoring the collection, transportation, extraction and amplification processes of a to-be-detected sample. According to the kit, the specificity is 100%, the detection sensitivity reaches 10 copies / mL, the specificity is good, and the detection efficiency is high. The kit is used for identifying the reproductive system disease pathogens of a to-be-detected sample, the to-be-detected sample can be vaginal secretion, the operation steps are simple, and the detection period is short.

Provided herein are methods of performing multiplex detection of ligand binding kinetics. In some embodiments, the methods include contacting ligands with an array of nucleic acid barcoded oscillators disposed on a first surface of a substrate that comprises an electrically conductive coating, applying an AC electric field to the substrate sufficient to induce the nucleic acid barcoded oscillators to oscillate proximal to the first surface of the substrate, and detecting changes in oscillation amplitudes of the nucleic acid barcoded oscillators over a duration to produce sets of ligand binding data. In some embodiments, the methods also include contacting barcode decoding nucleic acids with the array of nucleic acid barcoded oscillators applying an AC electric field to the substrate sufficient to induce the nucleic acid barcoded oscillators to oscillate proximal to the first surface of the substrate, and detecting changes in oscillation amplitudes of the nucleic acid barcoded oscillators over a duration to produce sets of barcode decoding data.

The invention relates to a kit for detecting a myasthenia gravis antibody and a preparation method of the kit. The kit for detecting the myasthenia gravis antibody comprises the following components: a myasthenia gravis antibody detection plate, a working solution, PBS (Phosphate Buffer Solution), a fluorescent secondary antibody and a mounting medium, wherein the myasthenia gravis antibody detection plate is prepared by mixing hela cells containing MUSK, LRP4, Titin and RYR1 proteins with modified polystyrene and then spotting the mixture on a glass slide; the modified polystyrene is obtained by reacting chloromethyl polystyrene resin with dopamine. According to the present invention, the existence of the four antibodies such as MUSK, LRP4, Titin and RYR1 can be simultaneously detected in the same system. The multiple detection mode can comprehensively reflect the antibody spectrum of the myasthenia gravis patient, reduce the sample size and the detection time, reduce the detection cost, and improve the detection efficiency and the clinical application value.

The invention discloses SSR (simple sequence repeat) multiple PCR (polymerase chain reaction) primers for paternity test of grass carp and application of the SSR multiple PCR primers. The primers comprise 16 pairs of specific primers, and base sequences of the 16 pairs of specific primers are respectively shown as SEQ ID NO.1-32 in sequence. The invention further discloses a fish paternity test kit comprising the SSR multiple PCR primer, an SSR multiple fluorescent PCR method for grass carp paternity test and application of the SSR multiple PCR primer, the kit or the method in grass carp paternity test. According to the kit, 16 pairs of specific SSR primers, multiple fluorescent PCR and a universal amplification primer technology are combined, 16 sites can be detected at a time, universal primers can be repeatedly used, and compared with simple single-site detection, the efficiency is improved, and the cost is reduced; the microsatellite loci contained in the invention are 3-6 basic groups, the allele size interpretation is more accurate, and the accuracy of genotype data is improved.

A multiplex KASP marker primer set for a set of wheat plant height major genes, consisting of a pre-primer having a nucleotide sequence as shown in SEQ ID NO. 10, a post-primer having a nucleotide sequence as shown in SEQ ID NO. 11, and a universal primer having a nucleotide sequence as shown in SEQ ID NO. 9. The multiplex KASP marker primer set achieves the simultaneous detection of the Rht-B1 and Rht-D1 genes.

The invention relates to the field of biological detection, in particular to a mini STR (short tandem repeat) double-multiplex amplification system and kit for degrading 25 gene loci of a sample. The invention provides a kit. The kit comprises 25 gene loci; the 25 gene loci comprise 23 autosomal STR gene loci, one sex gene locus and one Yindel gene locus. According to the kit provided by the invention, the primers are divided into a group A and a group B, and all gene loci are miniS TR gene loci on the basis of using current mainstream instruments and equipment. The two tubes of primers use the same experiment condition, and amplification and electrophoresis can be carried out at the same time. Compared with the existing miniSTR kit, the kit disclosed by the invention can be independently amplified and does not need to be combined with other autosomal STR kits for use, and the typing of 25 gene loci can be obtained through one-time detection. The effective detection rate of degraded samples is greatly improved, and technical support is provided for detection of difficult cases.

The invention discloses a primer and a method for detecting bacterial vaginitis related pathogens and application of the primer and the method. The nucleic acid sequence of the primer group comprises the sequences as shown in SEQ ID NO.1 to SEQ ID NO.12. The invention further discloses a kit for detecting the content of the nucleic acid sequence. The invention provides a primer group and a method for evaluating bacterial vaginitis related pathogens by designing specific primers to carry out super-multiplex PCR amplification and enrichment, obtaining the detection data volume of each pathogen based on sequence reading of a gene sequencing platform and assisting by a machine learning model, and further provides a kit for evaluating bacterial vaginitis related pathogens and a kit for evaluating bacterial vaginitis related pathogens. The problems that an existing Amsel and Nutant scoring method is greatly influenced by subjective factors, the requirement for the sample size of multiple qPCR joint detection is high, and the 16s rDNA detection cost is high can be solved.

The invention discloses a surface water quality parameter detection method, device and equipment, a medium and a product, and relates to the technical field of water quality monitoring. The method comprises the following steps: constructing sample data of each selected water quality parameter index according to ultraviolet-visible absorption spectrum data and water quality detection data of different surface water samples, sequentially determining a plurality of corresponding optimal water quality parameter estimation models and an optimal model fusion method on the basis of the corresponding sample data for each index, and calculating the optimal water quality parameter estimation model according to the optimal water quality parameter estimation model and the optimal model fusion method. Finally, the ultraviolet-visible absorption spectrum data, collected in real time, of the field surface water are imported into the optimal water quality parameter estimation models of all the indexes in real time, and the final index values of all the indexes of the field surface water are obtained by applying the optimal model fusion method of all the indexes. Therefore, the purpose of quickly, accurately and real-timely monitoring multiple parameters of the surface water quality can be achieved, and the method has the advantages of no reagent, no secondary pollution, real-time performance, high efficiency, multi-parameter monitoring capability and high robustness.

The invention provides a kit and a method for detecting common fusion genes of lymphoid leukemia based on a multiple digital PCR (Polymerase Chain Reaction) method. By constructing a synergistic effect mechanism of the primer and the fluorescent probe and optimizing matching of the primer, the probe and a fluorescent marker, non-specific binding among amplification products is effectively avoided. A composite reaction aid with a specific formula, namely a PCR enhancer, is innovatively introduced, so that the amplification efficiency and specificity of a multiple detection system are remarkably improved. According to the kit provided by the invention, the integrated detection capability on seven fluorescent channels, namely Atto 425, VIC, FAM, ROX, CY5, CY5.5 and CY7, is realized in a single-hole design. By innovatively constructing an ROX + CY5 and Atto425 + VIC dual-channel joint detection system, when the ROX and CY5 channels or Atto425 and VIC channels in the same detection hole simultaneously present positive signals, two additional detection targets can be specifically interpreted. The innovative detection strategy based on channel combination enables the kit to realize precise detection of 18 fusion genes only through two detection holes in a breakthrough manner.

The invention provides a primer probe composition and a kit for detecting a Databean virus and application, and relates to the technical field of biological detection, the primer probe composition comprises a primer probe group aiming at a Databean virus S gene and a primer probe group aiming at a Databean virus L gene, the Databean virus L and S genes are used as amplification target genes for design, and the kit is used for detecting the Databean virus. The primer probe group aiming at the S gene of the Databu virus and the primer probe group aiming at the L gene of the Databu virus are used as the primer probe composition, so that multi-gene target amplification is realized, and the positive detection rate reaches 100% and is higher than the positive detection rate of single-gene amplification; the lowest detection limit is up to 200 copies / mL; the quantification limit reaches 500 copies / mL, and the quantification linear range is 5.0 * 10 < 2 >-1.0 * 10 < 8 > copies / mL. The technical problem that in the prior art, a high-sensitivity and high-accuracy multiplex qPCR detection method for the nucleic acid of the copyda virus is lacked is solved.

The invention discloses a multiplex fluorescent PCR (polymerase chain reaction) kit and a detection method for multiple pathogenic microorganisms and drug-resistant genes. The kit comprises a primer and a fluorescent probe which are used for detecting target pathogenic microorganisms and drug-resistant genes, a PCR reaction premix solution, a positive quality control product and a negative quality control product. A plurality of fluorescent channels are used for respectively marking corresponding detection targets, and the amplification conditions of different targets are monitored by monitoring the change conditions of fluorescent signals in different fluorescent channels, so that the detection of 96 pathogenic microorganisms and drug-resistant genes is realized. The multiplex fluorescent PCR kit disclosed by the invention can be used for simultaneously detecting various pathogenic microorganisms and drug-resistant genes, and is high in accuracy, strong in specificity, high in sensitivity, rapid and reliable in result.

The invention relates to the technical field of biology, in particular to an anti-pollution freeze-dried eight-color Mini-STR composite amplification kit. Through design and optimization, the primer group, the reagent and the kit for detecting the eight-color multiple fluorescence labeling Mini-STR capable of identifying 25 STR gene loci are developed; the primer group, the reagent and the kit are used for detecting forensic samples, the detection sites are more, the detection accuracy is higher, the anti-pollution capacity is high, the detection capacity on trace samples is higher, the detection rate of degraded sample DNA can be remarkably increased, and the kit is higher in stability and has wide application value.

The present disclosure provides methods for conducting in situ multiplex and multi-omics detection and identification using coded padlocks probes. The methods comprise simultaneous use of RNA-specific padlock probes and polypeptide-specific padlock probes to detect both RNA and polypeptides in a cellular sample. Both types of probes include a barcode that unique identifies the RNA or polypeptide that that padlock probe detects. Both types of probes also include a batch-specific sequencing primer binding site to enable sequencing a desired subset of concatemer template molecules. Use of the batch-specific sequencing primers reduces overcrowding signals and images, to produces optical images that are intense and resolvable. By conducting multiple rounds of sequencing on the same cellular sample using different batch-specific sequencing primers enables multiplex and multi-omics sequencing to reveal numerous target RNAs and their encoded polypeptides.

The invention belongs to the field of biological detection, and particularly relates to an ewing sarcoma detection primer group, a kit and application thereof. The Ewing sarcoma detection primer group comprises a primer group A and a primer group B, and totally has 17 primer sequences. The primer group is designed, Ewing sarcoma characteristic variation genes are detected through multiple fluorescent PCR and a high-resolution capillary electrophoresis apparatus, 24 variation types fused by 7 types of genes can be detected at a time, the lowest detection limit is 10 copies, and the shortest detection time is 240 minutes. The detection result of the primer group disclosed by the invention can pass Sanger sequencing verification and is completely consistent with the morphological diagnosis result, the methodological consistency reaches 100%, and a sensitive and efficient Ewing sarcoma genetic variation detection method is provided.

The invention belongs to the technical field of plant virus detection, and provides a multiplex RT-PCR detection method for areca-nut related viruses. The primer group for multiple RT-PCR detection of areca-related viruses (areca yellow virus APV1, areca ringspot virus ANRSV and areca shuttle spot virus ANSSV) comprises a first primer group for detecting the APV1, a second primer group for detecting the ANRSV and a third primer group for detecting the ANSSV. The invention also provides a detection kit, which comprises a PCR reaction solution, and the PCR reaction solution comprises the primer group. The invention also provides application of the kit in simultaneous detection of areca-related viruses APV1, ANRSV and ANSSV. The primer group and the kit disclosed by the invention can specifically detect three areca-nut related viruses in a PCR system, can accurately identify any combination infection of the three viruses, and have the characteristics of high efficiency, economy, simplicity and convenience.

The invention provides a multiple qPCR (quantitative polymerase chain reaction) primer probe group for detecting duck enteritis virus, goose parvovirus and muscovy duck parvovirus, a detection system, a kit and application, and belongs to the technical field of virus detection. The multiplex qPCR detection primer probe group comprises an upstream primer DEV-F, a downstream primer DEV-R and a fluorescent probe DEV-Probe, wherein the upstream primer DEV-F, the downstream primer DEV-R and the fluorescent probe DEV-Probe are used for detecting the duck enteritis virus; the upstream primer GPV-F, the downstream primer GPV-R and the fluorescent probe GPV-Probe are used for detecting goose parvovirus, and the upstream primer MDPV-F, the downstream primer MDPV-R and the fluorescent probe MDPV-Probe are used for detecting muscovy duck parvovirus. The primer probe group provided by the invention has good specificity and sensitivity, can simultaneously detect three waterfowl infectious disease pathogens, has no cross reaction among the three pathogens, and is high in stability, and the coefficient of variation is below 10%.

Provided herein are methods, systems, compositions, and kits for detecting and decoding an encoded assay. The present disclosure provides low cost, multiplexed and automatable assays by hypercoding, a scalable technology for detection and quantitation of multi-omics targets. Hypercoding, or coding, utilizes signals from fluorescent hybridization with an error-corrected code to enable accurate detection and high-plexity targets of interest from samples. The present disclosure provides encoded assays for multiplex target detection from a sample suitable for detection by hybridization. Detection polynucleotide complexes, or detection oligonucleotides and anchor oligonucleotides, of the present disclosure are utilized in the detection and decoding of the hypercodes to produce optical signals capable of being decoded by a soft decision decoding algorithm for determining the presence of a target of interest, or multiple targets of interest, in a sample.

The invention belongs to the technical field of molecular identification of insects, and particularly relates to multiple PCR (Polymerase Chain Reaction) primers and a method for rapidly identifying thrips insect varieties. The invention discloses a multiple PCR primer and a method for rapidly identifying the variety of thrips, the identification primer and a multiple PCR system can rapidly, effectively and accurately identify the three types of thrips, and the sizes of PCR product strips of tobacco thrips, comb flower thrips and common large thrips are 104 bp, 237 bp and 439 bp respectively. The detection limit of the sensitive tobacco thrips is 500 copies, and the detection limits of the sensitive tobacco thrips and the sensitive common large thrips are both 100 copies. Compared with common PCR which can only identify one type of thrips at a time, the multiplex PCR system can complete identification of various types of thrips at a time, has the advantages of being easy to operate, low in cost, capable of saving time and the like, has very high value in the aspect of field thrips detection, and can be widely applied to the fields of plant protection and the like.

The invention provides a method for simultaneously detecting three hematogenous parasites based on RAA-LFS and application of the method. The method comprises the following steps: firstly, screening target genes of genomic DNA of specific amplified toxoplasma gondii, babesia and plasmodium and primers of the target genes; a primer and probe combination for specifically amplifying the 18s rRNA-1 gene of the toxoplasma gondii, the 18s rRNA-2 gene of the babesia and the 18s rRNA-3 gene of the plasmodium is determined; rapid multiple detection is completed in a reaction system by utilizing an RAA constant-temperature amplification technology, and a detection result is intuitively judged by combining a lateral flow chromatography test strip, so that the detection efficiency is greatly improved, and the result can be obtained in a short time. The RAA-LFS detection method provided by the invention has remarkable advantages in application scenes such as epidemic disease prevention and control or blood screening.

The invention provides a multiplex PCR (Polymerase Chain Reaction) detection primer composition for identifying streptococcus pasteurii serotypes and application of the multiplex PCR detection primer composition, and belongs to the technical field of biology. The multiplex PCR detection primer composition comprises a first primer pair, a second primer pair, a third primer pair and a fifth primer pair. The invention also provides application of the multiplex PCR detection primer composition in preparation of a kit for identifying the serotype of streptococcus pasteurii. The primer composition provided by the invention can identify five main serotypes of Pharmacococcus pasteurii through multiple PCR detection, and the detection method has the advantages of simple operation, strong specificity, high detection sensitivity, strong anti-interference performance and the like.

The invention provides a kit for single-hole detection of nucleic acids of various respiratory pathogens, and belongs to the technical field of pathogen detection, the kit comprises 2-5 primer combinations and a film-forming agent; the film-forming agent is polyethylene wax; each primer combination comprises a plurality of primer pairs, and the primer pairs are used for amplifying specific respiratory tract pathogen nucleic acid. According to the invention, the reaction is divided into two systems, the two systems are separated by a film-forming agent, amplification of the two reaction systems is carried out in the same reaction tube, after multiple amplification is completed, an isolating membrane is heated and melted, two groups of amplification products are mixed, and then the amplification products are detected. The multiplex RT-PCR kit which is good in detection specificity, high in sensitivity, good in repeatability and high in detection accuracy and can be used for simultaneously detecting various pathogenic bacteria is successfully constructed.

The invention provides a multiple qPCR (quantitative polymerase chain reaction) primer probe group for detecting a duck tembusu virus, a duck reovirus, a duck hepatitis A virus type 1 and a duck hepatitis A virus type 3, a detection system and application, and belongs to the technical field of virus detection. The multiplex qPCR detection primer probe group comprises an upstream primer, a downstream primer and a fluorescent probe which are used for detecting the duck tembusu virus, the upstream primer, the downstream primer and the fluorescent probe are used for detecting the duck reovirus; the upstream primer, the downstream primer and the fluorescent probe are used for detecting the duck hepatitis A virus type 1, and the upstream primer, the downstream primer and the fluorescent probe are used for detecting the duck hepatitis A virus type 3. The primer probe group disclosed by the invention has good specificity and sensitivity and can be used for simultaneously detecting four waterfowl infectious disease pathogens.

The present invention provides multiplex assays for improved scoring of tumour tissues stained with an immune checkpoint protein such as VISTA. Methods of ranking multiple tumour tissues and of diagnosing cancer patients are also provided. The methods disclosed are also useful for identifying patient responsive to anticancer therapy.

Disclosed is a multiplex quantum dot-based quantitative test card, and a preparation method, apparatus, and use method thereof in the technical field of biological testing, the multiplex quantum dot-based quantitative test card including a multiplex card housing and a test strip, where the multiplex card housing is provided with a plurality of reaction slots configured to accommodate the test strip; the multiplex card housing further includes a plurality of sample loading ports and a plurality of observation windows, where the sample loading ports and the observation windows are in one-to-one correspondence with the reaction slots; and the test strip includes a conjugate pad coated with quantum dot-labeled antibodies, where the quantum dot-labeled antibodies serve as chromogenic substances.

The invention relates to the technical field of life science, in particular to a method for constructing an atrophic gastritis-based SPEM lesion model, which comprises the following steps: S1, constructing an Slc7a11 conditional knockout mouse strain: carrying out cage hybridization on an Slc7a11 flox / flox mouse and an ATP4b-Cre transgenic mouse, and knocking out a gastric wall cell specific Slc7a11 gene; s2, carrying out genotyping identification; the identification method based on the atrophic gastritis SPEM lesion model and the construction method based on the atrophic gastritis SPEM lesion model comprise the following steps: S1, pathological model verification: taking a mouse gastric mucosa tissue slice, quantitatively analyzing the proportion of an SPEM region by adopting an immunofluorescence double-standard technology, and confirming Slc7a11 knockout induced SPEM pathological characteristics in combination with the existence of SPEM in the gastric mucosa; and S2, long-term pathology evolution monitoring. Through gastric wall cell specific Slc7a11 gene knockout and multiple PCR identification technologies, the initial response SPEM state after gastric mucosa damage is successfully simulated, and the kit is used for researching atrophic gastritis pathological mechanisms and evaluating drug intervention.

The invention designs a carbapenem drug-resistant gene nucleic acid fluorescent PCR method detection kit and application thereof, the kit uses Taqman fluorescent probe multiple amplification technology to detect carbapenem drug-resistant genes, the kit comprises a nucleic acid reaction liquid Mix1 and a nucleic acid reaction liquid Mix2, the nucleic acid reaction liquid Mix1 is used for detecting drug-resistant genes IMP, NDM and OXA48, the nucleic acid reaction liquid Mix2 is used for detecting drug-resistant genes IMP, NDM and OXA48, and the nucleic acid reaction liquid Mix2 is used for detecting drug-resistant genes IMP, NDM and OXA48. The nucleic acid reaction liquid Mix2 is used for detecting drug resistance genes VIM, KPC and OXA23. The method is high in detection specificity, and the detection result is visual and easy to interpret. Clinical doctors are assisted to judge respiratory tract pathogen infection more comprehensively and more accurately. The kit covers six carbapenem drug resistance genes, is more comprehensive and accurate in detection, and is high in specificity and sensitivity. Meanwhile, multiple sample types can be detected, a sputum sample, an excrement sample and a rectum swab sample are detected through paramagnetic particle extraction, and pure bacterial colonies are directly detected without extraction.

The invention relates to the technical field of molecular diagnosis, in particular to a multiplex nucleic acid detection primer probe combination and application thereof. A corresponding primer probe combination is designed for researching 10 common respiratory tract pathogens such as new coronavirus, influenza A virus, influenza B virus, adenovirus, rhinovirus, respiratory syncytial virus and human parainfluenza virus types 1, 2, 3 and 4, a respiratory tract infection multiple pathogen detection system is established, a multiple RT-qPCR system is optimized, and the kit is used for detecting the respiratory tract infection multiple pathogens. The optimized system is applied to a clinical throat swab sample for verification, and the multiple pathogen nucleic acid detection kit is researched and developed and used for detecting viral respiratory tract infection. According to the invention, multiple pathogens are detected by adopting a multiple RT-qPCR method, so that the detection time can be shortened, the detection sensitivity and specificity are improved, the detection cost is reduced, and the kit has important clinical significance on rapid diagnosis of respiratory tract multiple pathogen infection.