11335 results about "Reagent" patented technology

The disclosure provides systems and methods for droplet processing. For example, a method can include providing a first droplet and a second droplet. In some cases, the first droplet may have a first concentration of a reagent and the second droplet may have a second concentration of the reagent. The second droplet may comprise a bead or a biological particle. The method can also include subjecting the first droplet and the second droplet to conditions sufficient to transfer the reagent from the first droplet to the second droplet, thereby decreasing the first concentration in the first droplet and increasing the second concentration in the second droplet.

The invention relates to the technical field of food, in particular to diglyceride for reducing triglyceride, cholesterol and uric acid as well as a biological enzyme preparation method and application of the diglyceride. The preparation method comprises the following steps: (1) adding water and glycerol into vegetable oil, and then adding immobilized lipase to react to obtain a reactant; (2) centrifuging reactants, collecting a light-phase product, carrying out molecular distillation, and collecting a heavy-phase product to obtain diglyceride; the immobilized lipase comprises lipase and a carrier, the carrier comprises loofah sponge, soybean meal and bentonite, and the mass ratio of the loofah sponge to the soybean meal to the bentonite is 1: (2-4): (3-6). The method has the advantages that the effects of reducing triglyceride, total cholesterol and uric acid are relatively good, and the yield and the purity are relatively high.

The invention relates to a preparation method of Pt monatomic nano-enzyme and application of Pt monatomic nano-enzyme in cardiac troponin I. The preparation method comprises the following steps: 1) ultrasonically dispersing cobalt salt, selenium powder, chloroplatinic acid, urea and sodium chloride in an alcohol solvent, heating and stirring until the solvent is completely volatilized to obtain a solid mixture, and grinding the solid mixture into powder; and 2) placing the powder in a heating device, carrying out Joule heat treatment in an air atmosphere, washing a reactant, and carrying out vacuum drying to obtain the Pt monatomic nano-enzyme. Se doping is introduced in the one-step chemical reaction process to construct oxygen vacancies, rapid heating and structural shaping are achieved in combination with Joule heat treatment, the Se-doped Co3O4-loaded Pt monatomic catalyst is obtained, the oxygen vacancies and Se doping sites are synergistically used as anchoring sites, Pt monatomic atoms are effectively fixed, and the loading capacity and dispersity of the Pt monatomic atoms are improved. The obtained material shows excellent POD-like enzyme activity, can be used for constructing a high-sensitivity colorimetric immunoassay system, and realizes rapid and accurate detection of cTnI.

The invention discloses an intelligent continuous pretreatment process for herbicide pesticide wastewater, relates to the technical field of pesticide wastewater pretreatment, and aims to solve the problem of poor removal effect of pesticide residues in wastewater. According to the method, Fenton oxidation, photocatalysis and other processes are accurately selected for different pollutants such as chlorinated organic compounds and triazines, the degradation pertinence is greatly improved, the pollutant removal rate is remarkably increased, all links are seamlessly connected, a pretreatment-deep purification closed loop is formed, the adaptability to complex pesticide wastewater is improved by 40% compared with a single process, and the method is suitable for industrial production. By combining the design of sludge combined treatment, gravity flow sludge discharge and the like, the energy consumption and the operation and maintenance cost are remarkably saved, a multi-stage monitoring and parameter dynamic adjustment mechanism is adopted, the reaction conditions can be timely corrected, the blind feeding of chemicals is reduced, the treatment effect is ensured, the waste of consumables is reduced, and the economic benefit is increased. Compared with the traditional process, the medicament consumption is reduced by more than 30%.

The invention relates to the technical field of biomedical ceramic materials, in particular to a zirconia-based biomedical ceramic material and a preparation method thereof.The preparation method comprises the following steps: preparing a zirconia-based biomedical ceramic material on the basis of preset opening degrees of air channels of a first heating part and a second heating part in a heating assembly and a preset working state of a first stirring structure; synchronously and continuously adding a plurality of to-be-reacted substances into the first space of the container for primary reaction; after the interval is fixed, opening a circulation channel based on a preset liquid channel opening degree of the circulation channel of the first space and the second space of the container, so that the material in the first space flows into the second space, and performing a second-section reaction at a preset second temperature and a preset rotating speed of a second stirring structure to generate a precipitate; according to the invention, the materials to be reacted can be heated at a stepped temperature, and the reaction process is fully adjusted according to the ion concentration and uniformity in the detected solution, so that the high-precision preparation of the zirconium oxide-based biomedical ceramic material is stably and efficiently carried out.

The invention relates to the technical field of bearings, and provides a hydrostatic bearing gas supply system which comprises a first gas supply subsystem and a second gas supply subsystem, the first gas supply subsystem comprises a first pipeline, a first check valve, a first reaction mechanism, a second reaction mechanism and a degassing structure, and the first reaction mechanism, the second reaction mechanism and the first pipeline are sequentially connected; a reactant is arranged in the first reaction mechanism, a catalyst is arranged in the second reaction mechanism, and the first check valve and the degassing structure are arranged on the first pipeline; the second gas supply subsystem is connected with the first pipeline; when the gas source is cut off, the reactant and the catalyst are subjected to chemical reaction in the second reaction mechanism to generate water vapor and oxygen, the degassing structure is used for consuming the oxygen and generating superheated steam, and the superheated steam enters the hydrostatic bearing. The air supply system for the hydrostatic bearing provides the pure water vapor working medium for the hydrostatic bearing, so that the normal operation of the hydrostatic bearing is ensured, and the hydrostatic bearing is prevented from being damaged.

The invention provides a composite reagent system and a chalcopyrite and sphalerite collecting method, and belongs to the technical field of mineral processing. The composite reagent system comprises a histidine derived functional group collecting agent and a black agent, and the histidine derived functional group collecting agent (containing a total sulfur ester group, a sulfydryl functional group and a side chain imidazole ring) serves as a core and is compounded with the black agent according to a specific proportion to form a target combination-synergistic interaction flotation mechanism. The histidine derivative collecting agent derives high-activity functional groups under the alkaline condition, and after sulfydryl is dissociated, stable chemical bonds are formed by the dissociated sulfydryl and metal ions on the surfaces of the chalcopyrite and the zinc sulfide. The black powder serves as an auxiliary collecting agent, and the collecting capacity of fine-grain or low-activity sulfide minerals is enhanced through chemical adsorption of dithiophosphate radicals and metal ions on the surfaces of the chalcopyrite and the sphalerite. After the two components are compounded, the selective enrichment efficiency of copper-zinc minerals in the flotation process is remarkably improved.

The invention provides a TEM sample and a preparation method and application thereof.According to the preparation method of the TEM sample, on the premise that the hole structure of a semiconductor device is not affected, part of residual polymer accumulated and blocked in a hole opening in the semiconductor device is removed through reactive ion etching, so that the hole opening is expanded and not blocked any more; a protective colloid can enter a pore structure for filling and precursor material plating of atomic layer deposition is facilitated, the definition of the side wall of the pore is enhanced, and the quality and the final observation effect of the TEM sample are effectively improved; the equipment and technology adopted by the preparation method of the TEM sample provided by the invention are low in investment, and the operation process is simpler and faster than that of a Fab etching process; meanwhile, batch laboratory-scale sample treatment can also be realized; in addition, compared with a wet chemical corrosion cleaning technology for removing residual polymers, the problems of chemical reagent residues, chemical reactant pollution and the like at the bottom of the deep hole structure are avoided.

The invention discloses a preparation method of an acid-resistant polyquaternary ammonium membrane based on a Porxitch interfacial polymerization reaction. The preparation method comprises the following steps: dissolving an amine monomer in a water-phase solvent to form a water-phase solution; dissolving a bromomethyl monomer in an organic phase solvent to form an organic phase solution; after the base membrane is soaked in the water phase solution for a period of time, the water phase solution is removed, and the water phase solution remaining on the surface of the base membrane is removed; coating the organic phase solution on the surface of a base membrane, and carrying out interfacial polymerization reaction for 1-15 minutes at the high temperature of 50-90 DEG C, so as to prepare a polyquaternary ammonium membrane on the base membrane; and washing the surface of the polyquaternary ammonium membrane with an organic solvent to remove residual reactants, and then carrying out heat treatment in a drying oven to obtain the acid-resistant polyquaternary ammonium membrane.

The invention relates to the technical field of high polymer material chemistry, and discloses a high and low temperature resistant anti-yellowing optical adhesive and a preparation process thereof, the high and low temperature resistant anti-yellowing optical adhesive comprises the following components by weight: 40-60% of modified organic silicon resin, 20-30% of urethane acrylate, 10-15% of alicyclic epoxy resin, 0.5-5% of a nano inorganic filler, 0.8-2.5% of an anti-yellowing composite reagent, and the balance of a solvent. The coating is prepared from the following components in percentage by weight: 0.5 to 1.5 percent of 2-hydroxy-2-methyl-1-phenyl-1-acetone, 0.1 to 0.3 percent of fluorine modified polyacrylate flatting agent and 0.3 to 1.0 percent of chloroplatinic acid-divinyl tetramethyl disiloxane complex catalyst. The preparation method comprises the following steps: mixing alpha, omega-dihydroxy polydimethylsiloxane and phenyltrimethoxysilane according to a mass ratio of 8: 2-7: 3, adding 0.3-1.0% of a chloroplatinic acid-divinyl tetramethyl disiloxane complex catalyst, and raising the temperature by stages under the protection of nitrogen, so that the glass transition temperature of the adhesive can reach 120 DEG C below zero and 125 DEG C, and the temperature of the adhesive can reach 120 DEG C below zero and 125 DEG C below zero. And the long-term service requirement of the optical device in an extreme temperature environment is met.

The present disclosure relates to compositions and methods for reversible fixation of biological samples using fixation reagents that form bis-carbamate crosslinks between amine-bearing moieties in biomolecules.

A method for visible light-mediated chemical modification of a polypeptide or protein based on cysteine includes the following steps: allowing a cysteine residue on a cysteine-containing oligopeptide, polypeptide, or protein to undergo a reaction with an activating reagent to generate a free radical precursor in situ; and subjecting the free radical precursor to desulfurization under photocatalytic condition, and allowing the generated free radical intermediate to undergo an addition reaction with an olefin or an alkyne, such that sulfhydryl removal and carbon-carbon bond construction are achieved to produce a chemical modification product of the polypeptide and protein. The method cleverly avoids the influence on the chiral center, and thus the chirality of the amino acid residue can be retained. The method is conducive to well avoiding the occurrence of side reactions, and thus the efficiency of the entire conversion is very high.

The invention provides a chiral resolution method and application of a 3-aryl substituted glutaric acid monoester compound, and the method comprises the following steps: taking a substance as shown in a formula I as a starting raw material, and carrying out alcoholysis to obtain a racemic intermediate as shown in a formula IV; and splitting the intermediate shown in the formula IV, and then dissociating to obtain the compound shown in the formula III, which has a single spatial configuration and an ee value of more than 99%. A cyclic anhydride substrate with a symmetrical structure is used and reacts with alcohol to obtain racemic 3-aryl substituted glutaric acid monoester, a target configuration with high chiral purity is obtained by screening some resolution reagents and solvents and performing salifying resolution on the 3-aryl substituted glutaric acid monoester, the operation is simple, the resolution reagents are easily available in the market and can be recycled, and the method is suitable for industrial production. The method has the advantages that an expensive chiral catalyst or a harsh enzyme catalysis condition is avoided, the reaction cost is reduced, amplified production is facilitated, the ee value of a target configuration can be increased to 99% or above, and the method is an economic and environment-friendly technical route which is simple in post-treatment and convenient for amplified production.

The invention provides a preparation method and application of a wear-resistant polyimide material, and the preparation method comprises the following steps: S1, adding a polyimide material into an alkali solution for reaction, cleaning reactants with acid and ethanol, and drying to obtain an activated polyimide material; s2, adding the activated polyimide material into a silane coupling agent solution for reaction, cleaning reactants with ethanol and water, and drying to obtain a modified polyimide material with silicon-oxygen bond active sites on the surface; s3, adding the modified polyimide material with silicon-oxygen bond active sites on the surface into a modified epoxy resin solution, adjusting the pH value to be acidic, and reacting to obtain the wear-resistant modified polyimide material, wherein the modified epoxy resin solution is obtained by reacting a silane coupling agent with acrylate. According to the method, the wear resistance of the polyimide can be improved on the premise that other properties of the polyimide material are not influenced.

The invention provides a dump leaching method for improving the leaching rate of gold ore, and belongs to the technical field of mineral processing. The method comprises the following steps: firstly, separating minerals into oversize products and undersize products; mixing sodium carboxymethyl cellulose, bentonite and ferrous sulfate to obtain a mixture, and granulating the undersize product by using the mixture as a binder; and mixing the undersize product particles with the oversize product, and carrying out cyanide-free dump leaching. According to the method, through graded granulation and heap building, the problem that heap building permeability is poor due to the fact that the gold ore contains many clay minerals is solved. In the granulation stage, ferrous hydroxide colloid generated by hydrolysis of ferrous sulfate in the binder can assist ore particle agglomeration, and particle stability is enhanced; during subsequent cyanide-free dump leaching, ferrous sulfate can be used as an auxiliary reducing agent and a catalyst in a thiourea gold leaching system to promote oxidation and dissolution of gold; meanwhile, ferrous ions can adjust the oxidation-reduction potential of a system, so that the thiourea gold leaching reaction is in a more favorable potential interval, the oxidation efficiency of thiourea is improved, invalid decomposition of thiourea is reduced, and therefore the use amount of thiourea and the use amount of agents are reduced.

The invention discloses a preparation method of second-generation denitrified flavin TND1128, which comprises the following steps: S1, by taking 1-methylbarbituric acid and o-aminobenzaldehyde as raw materials and a mixture of water and alcohol as a solvent, heating and reacting to generate an intermediate I; s2, taking the intermediate I as a raw material, and performing ethylation under the combined action of inorganic alkali and an ethylation reagent to generate a TND1128 crude product; s3, the TND1128 crude product is purified, and a TND1128 pure product is obtained; according to the invention, 1-methylbarbituric acid and 2-nitrobenzaldehyde are taken as raw materials for cyclization synthesis of an intermediate I, then ethylation and pulping purification are carried out under the combined action of inorganic base and an ethylation reagent, and high-purity TND1128 is synthesized in a high-yield, high-specificity and high-selectivity manner; the method has the advantages of simple process route, good reaction selectivity, high yield, cheap and easily available raw materials, low equipment requirement, easy realization of industrial production and the like.

The invention discloses a magnetic ion exchange resin and a preparation method thereof, and the preparation method comprises the following steps: preparing a reaction solution which comprises magnetic particles, glycidyl methacrylate, divinylbenzene and a photoinitiator; the mass ratio of the glycidyl methacrylate to the divinyl benzene is (4.5-10): 1; an ultraviolet-initiated polymerization step: irradiating the reaction solution by ultraviolet light to initiate a polymerization reaction to obtain magnetic microspheres; a functional modification step: mixing the magnetic microspheres with an ammoniation reagent for ammoniation reaction to prepare ammoniated magnetic microspheres; and mixing the ammoniated magnetic microspheres with an alkylation reagent, and carrying out an alkylation reaction to prepare the magnetic ion exchange resin. According to the method, the production time is remarkably shortened, the extra energy consumption is reduced, and the process production flow is effectively simplified.

The application provides a masterbatch type vulcanization aid and a preparation method thereof, a rubber composition, a rubber product and a tire. The preparation method comprises the following steps: S1, pre-reaction of stearic acid, an antioxidant and a vulcanization system to obtain a pre-reaction mixture; and S2, mixing of the pre-reaction mixture with rubber to obtain the masterbatch type vulcanization aid. Under the catalysis of stearic acid, the reagents in the antioxidant and the vulcanization system react to generate active intermediates. The active intermediates have the effect of promoting vulcanization and play the role of activating accelerators in advance, so that the active intermediates can replace the role of zinc oxide. The product after reaction is mixed with rubber, and the masterbatch formed can be used to prepare rubber products such as tires without containing zinc elements, so that zinc emission can be realized, and the environment is friendly.

The invention discloses a method for predicting the catalytic activity of a bimetallic supported catalyst in a CO2RR reaction, and belongs to the technical field of computational material science and catalytic materials. The method comprises the following steps: constructing a spatial configuration of TMCu / W2CO2 with double metal atoms (TMCu) loaded on the surface of W2CO2; a relaxation configuration with the distance between TM atoms and Cu atoms smaller than 3.0 is screened out; calculating the adsorption energy Eads of adsorbing CO2 at different sites, and screening out a catalyst model with strong adsorption capacity on reactants; the optimal path in the reaction process is determined, the limiting potential UL of the surface of the catalyst is calculated based on the maximum value of the Gibbs free energy change delta G in the optimal path, and the UL is used for predicting the activity of different TMCu / W2CO2 surface electrocatalysis CO2RR. According to the method, screening standards taking the distance between double metal atoms, the binding energy of double metals, the adsorption energy of CO2 and the limited potential as the core are established, a low-efficiency system is rapidly eliminated, the experiment trial and error cost is reduced, and the development efficiency of the catalyst is improved.

The invention discloses a micro-reagent ammonia nitrogen on-line continuous analyzer and an analysis method, and belongs to the technical field of water quality ammonia nitrogen monitoring, the micro-reagent ammonia nitrogen on-line continuous analyzer comprises a first injection pump, a second injection pump, a multi-channel valve, a liquid storage ring and a reaction tank; liquid outlets of the first injection pump and the second injection pump are respectively connected with one end of the liquid storage ring and a circulating port of the reaction tank through different channels of the multi-channel valve; a circulating port of the reaction tank is connected with a first peristaltic pump; the other end of the liquid storage ring is connected with a second peristaltic pump; liquid outlets of the first injection pump and the second injection pump are connected with a third peristaltic pump; a first reagent and a second reagent for ammonia nitrogen analysis are respectively stored in the first injection pump and the second injection pump; different channels of the multi-channel valve are respectively connected with a standard sample and a water sample which are used for ammonia nitrogen analysis, and cleaning water. The micro-reagent ammonia nitrogen on-line continuous analyzer provided by the invention can reduce cross contamination of reagents, reduce reagent consumption and realize short-interval continuous analysis.

The invention provides a thioether oxidation catalyst, a preparation method thereof and a preparation method of pyroxasulfone. The preparation method comprises the following steps: S1, preparing a selenium source and a first solvent into a first solution; s2, adding a Lewis acid source into the first solution, and performing first mixing to obtain a second solution; s3, adding an organic alkali ligand source into the second solution, and performing second mixing to obtain a third solution; and S4, carrying out solvothermal reaction on the third solution at 100-150 DEG C to obtain the thioether oxidation catalyst. A selenium source, a Lewis acid source and an organic alkali ligand source are subjected to a combined reaction according to a specific sequence and conditions, and finally the inorganic-organic hybrid selenite catalyst with a special structure is formed under a solvothermal condition. The obtained catalyst is high in active atom utilization rate and strong in reactant molecule adsorbability, is beneficial to deep oxidation of reactants, accelerates oxidation of thioether into sulfone, and improves reaction selectivity and production efficiency.

A fully automated chemiluminescence immunoassay analyzer, including a sample and reagent receiving device for receiving a sample and a reagent, a dispensing device for aspirating and discharging the sample and the reagent, a mixing device for mixing the sample and the reagent in a reaction vessel, an incubation and luminescence detection device for incubation and luminescence detection, a magnetic separation cleaning device for separation cleaning an analyte and impurities in the reaction vessel, a reaction vessel grasping device for transferring the reaction vessel, and a liquid path device.

The invention relates to the field of organic chemical synthesis, in particular to a synthetic method and application of pyrimidone compounds. The invention provides a synthesis method, reactants are o-amino heterocyclic formamide and triethyl orthoformate respectively, a solvent system is a hexafluoroisopropanol solvent, and a product is a heterocyclic pyrimidone compound. The reaction does not need strong acid or strong alkali conditions, does not need additional reducing agents or oxidizing agents, does not need transition metal catalysis, is economical and practical, the reaction conditions are relatively mild, and the synthesized pyrimidone compound has a good antibacterial effect.

The invention discloses a method for preparing calcium hydroxide slurry with high specific surface area and high pore volume, which comprises the following steps: S1, adding an alcohol reagent into water to obtain a solution A; s2, adding an inorganic salt solution into the solution A, and uniformly mixing to obtain a solution B; s3, a reagent X is added into the solution B, ultrasonic mixing is performed to obtain a solution C, and the reagent X is a mixture of phenol or triethanolamine, ethidene diamine and ethylamine; and S4, adding calcium oxide powder into the solution C, continuously stirring, and reacting to obtain calcium hydroxide slurry. The invention provides a method for preparing calcium hydroxide slurry with high specific surface area and high pore volume, the calcium hydroxide slurry prepared by the method can form calcium hydroxide with high specific surface area and high pore volume when in use, and the method aims to solve the problems of low specific surface area and low pore volume of the existing calcium hydroxide. Therefore, the problem of desulfurization performance inactivation caused by blockage of calcium hydroxide (desulfurizer) pores by generated CaSO3 and CaSO4 in the desulfurization process is avoided.

The invention belongs to the technical field of adhesives, and particularly relates to a preparation method of a polysaccharide-based reversible bonding adhesive for constructing a dynamic covalent bond network. The preparation method comprises the following steps: by taking lipoic acid and sodium alginate as reactants and ferric chloride as a cross-linking agent, carrying out cross-linking reaction under a stirring condition to obtain a lipoic acid adhesive, namely the polysaccharide-based reversible bonding adhesive. According to the invention, renewable biomass resources such as sodium alginate, hyaluronic acid, chitosan and the like are used as main raw materials, so that the adhesive is expected to replace a traditional synthetic adhesive depending on a petroleum base, accords with a sustainable development strategy, contributes to reducing consumption of fossil resources, avoids release of toxic substances such as formaldehyde and the like in the synthesis and use processes, and has a good application prospect. And the method is more environment-friendly. The bio-based polysaccharide raw material has good biocompatibility and degradability, and an ideal base material is provided for developing a green and safe biomedical adhesive.

The application provides a kind of super-long chemical pipeline conveying system, comprising: centralized liquid supply system equipment;Several chemical distribution plates;Several transparent PVC boxes, and hollow double-layer pipes;For connecting centralized liquid supply system equipment, chemical distribution plate and transparent PVC box;Hollow double-layer pipe includes transparent PVC pipe and PFA hose, transparent PVC pipe is contained in transparent PVC direct head, PFA hose is contained in transparent PVC pipe, PFA hose diameter is 1-1.5 inch, transparent PVC box is internally provided with by letting box, by letting box includes several adapters, locking nuts and electronic valves, electronic valve is electrically connected with PLC and smoke sensor;Transparent PVC box bottom surface is also provided with several reagent boxes, and reagent box contains reagent that can react with dangerous chemicals to generate smoke.The application can realize super-long chemical transportation turning to prevent pipeline rupture, and prevent chemical leakage.

The invention relates to a vertical flow enhanced biological denitrification device and a sewage treatment method, a denitrification agent adding assembly is additionally arranged at a proper position of an existing vertical flow reaction device, namely the outer side of an outer inclined flow guide part, a corresponding enhanced denitrification agent can be added at a selected point position, a denitrification reaction is further enhanced, and the denitrification effect is improved. The selective reaction of the functional microorganism reaction is improved, nitrate generated by the anaerobic ammonia oxidation reaction is consumed, and a sufficient matrix is provided for anaerobic ammonia oxidation, so that the anaerobic ammonia oxidation reaction is greatly promoted, and the total nitrogen removal rate is further improved; by accurately controlling the dosing position and accurately dosing according to the reaction, the nitrogen removal efficiency of the autotroph is enhanced, the coordination of the minimization of the dosing amount of the medicament and the maximization of the nitrogen removal efficiency is realized, and finally, the green, low-carbon, economical and efficient nitrogen removal target of the sewage is achieved.

The invention discloses a carbon nitride-based composite material as well as a preparation method and application thereof. The carbon nitride-based composite material comprises a non-metal doped carbon nitride carrier and a noble metal, wherein the noble metal exists in the carrier in an element doping form and / or is loaded on the carrier in an oxide form; the precious metal is one or more of Pt, Pd, Au and Ru, and the nonmetal is one or more of P, S and I. According to the carbon nitride composite material disclosed by the invention, noble metal is utilized to modify carbon nitride, the number of surface active sites is increased, the electron and chemical structures of an active center are adjusted, and meanwhile, generation and migration of photo-generated charges are promoted. Meanwhile, the surface of the carbon nitride composite material is modified by non-metallic elements, so that adsorption and activation of reactant H2O molecules are effectively promoted. The catalyst disclosed by the invention is simple in preparation process, good in repeatability and easy for large-scale production.

The utility model belongs to the technical field of micro-fluidic chips, and particularly provides a digital MIRA real-time diagnosis chip and an analysis device.The digital MIRA real-time diagnosis chip comprises a top glass slide, a micro-pattern layer and a bottom glass slide which are sequentially arranged from top to bottom; a water-soluble film is embedded in a position where the micro-pattern layer is matched with the water filling nozzle; the sample injection port is suitable for sequentially injecting a reaction reagent and sealing oil. Through the design of a chip structure without an outlet, when reaction reagents are introduced from a sample injection port, all the reaction reagents simultaneously, equivalently and uniformly enter each micro-chamber, and the reaction reagents are separated into each micro-chamber by adopting an oil seal mode, so that the chip has the advantage of 100% sample discretization in the digital nucleic acid detection process, and no sample or reagent is lost. Meanwhile, each part of reaction reagent is accurately separated into each micro-chamber and cannot be pushed out of the chip, so that each molecule enters the corresponding micro-chamber, and the detection requirement of a low-concentration template is met.

The invention relates to the technical field of bearings, and provides a hydrostatic bearing air supply system capable of improving transient bearing capacity, which comprises a first air supply subsystem and a second air supply subsystem, the first gas supply subsystem comprises a first pipeline, a first switch valve, a reaction mechanism and a degassing mechanism, reactants are arranged in the reaction mechanism, the reaction mechanism is communicated with the first end of the first pipeline, the second end of the first pipeline is used for being communicated with the hydrostatic bearing, and the first switch valve and the degassing mechanism are both arranged on the first pipeline; the degassing mechanism is positioned between the reaction mechanism and the first switch valve; the second air supply subsystem is used for communicating with the hydrostatic bearing through a first pipeline; the first switch valve is used for being opened under the condition that the hydrostatic bearing is impacted, so that water vapor in the hydrostatic bearing enters the reaction mechanism to be chemically reacted with reactants, and water vapor and oxygen are generated; the degassing mechanism is used for consuming oxygen and generating superheated steam. Normal operation of the hydrostatic bearing can be guaranteed, and the hydrostatic bearing is prevented from being damaged due to rotor collision.