14131results about "Bioreactor/fermenter combinations" patented technology

A system for the hypothermic transport of biological samples, such as tissues, organs, or body fluids. The system includes a self-purging preservation apparatus to suspend a sample in preservation fluid and perfuse a tissue with preservation fluid. The self-purging preservation apparatus is placed in an insulated transport container having a cooling medium. When assembled, the system allows for transport of biological samples for extended periods of time at a stable temperature.

The invention relates to the technical field of microbial separation, in particular to an efficient targeted separation device and method for environmental functional microorganisms. The device comprises an environmental sample preset culture module and an intelligent detection and feedback regulation module which are arranged in a box body, the preset culture module is used for culturing a pretreated target environmental microorganism sample, the intelligent detection and feedback regulation module is used for detection every certain period of culture, and the sample is obtained through automatic separation until the sample is completely cultured. The device can realize efficient culture and separation of target environmental functional microorganisms, avoids sample loss and pollution risk, remarkably improves the standardization degree and efficiency of the culture process, and solves the problems of low efficiency, poor accuracy, limited separation quantity and insufficient standardization of environmental functional microorganisms in the prior art.

The invention belongs to the field of microbial electrochemistry and solid organic waste recycling, and discloses a microbial electrosynthesis enhanced solid wastewater hydrolysis and anaerobic digestion biogas high-value utilization system coupling microbial electrosynthesis, organic solid waste electrochemical-biological synergistic hydrolysis oxidation and anaerobic digestion. The system comprises a cathode chamber and an anode chamber which are separated by an ion exchange membrane, an electrochemical driving device and an anaerobic digestion unit communicated with the anode chamber. And the cathode chamber is provided with a cathode electrode and an anaerobic acetic acid production microbial system and is communicated with biogas generated by anaerobic digestion, so that carbon dioxide in the biogas is reduced at the cathode to generate acetic acid and methane-enriched gas is output. The anode chamber is provided with an anode electrode, organic solid waste and a hydrolytic microorganism system are added, electrochemical-biological synergistic hydrolytic oxidation of the organic solid waste and hydrolysates of the organic solid waste is used as an anode reaction, oxygen evolution is inhibited, and oxygen transmembrane migration is reduced; and the hydrolysate in the anode chamber flows into the anaerobic digestion unit to produce biogas, so that the integrated operation of solid wastewater hydrolysis and biogas upgrading is realized.

The invention relates to the technical field of industrial process control, and discloses a digital twinning-based fermentation process temperature adaptive control system, which comprises a data acquisition and state representation module, a probability generation type digital twinning module, an adaptive risk management module, an opportunity constraint model prediction control module and a model iteration trigger module, according to the method, probability distribution of future states is predicted by constructing probability generation type digital twinning, and the uncertainty of the model is quantified in real time; the system can dynamically adjust the risk tolerance, drives the chance constraint model prediction controller, seeks an optimal control solution on the premise of ensuring the process safety probability, triggers the model adaptive update by means of an online evaluation mechanism, and realizes the optimal control solution on the basis of effectively managing the process uncertainty. The fermentation temperature is subjected to high-performance self-adaptive control considering safety and economy, and the problem that a traditional deterministic model is insufficient in control robustness is solved.

Methods of and system for growing and maintaining an optimized / ideal active yeast solution in the yeast tank and fermenter tank during the fermentation filling cycle are provided. A new yeast stage tank is used between the yeast tank and the fermenter tank allowing yeast to rapidly produce a huge amount of active young yeast cells for a fermenter during the filling period. A measurable and useful controlling factor, % DT / % Yeast by weight ratio (or “food” to yeast ratio), is used (e.g., % DT=glucose), which offers information on the health status of the yeast. The controlling factor is used to control the status of the yeast throughout the entire process.

The invention relates to the technical field of tumor biomedical engineering and organ chips, in particular to a vascularized gastric cancer organ chip and a preparation method thereof.The chip is composed of an integrated micro-fluidic main body, an annular micro-column array and an optical sealing film, and a central culture cavity is divided into a tumor area and a blood vessel area by micro-columns; the preparation method comprises the following steps: injecting a fibrous protein solution containing human umbilical vein endothelial cells and cancer-related fibroblasts into a vascular region, and adding thrombin for in-situ gelation to form a pre-vascularized network; the method comprises the following steps: mixing a patient-derived gastric cancer organ with a methacrylic acid esterified gelatin pre-polymerized solution, injecting the mixture into a tumor area, and carrying out photo-crosslinking immobilization; culturing for 7-14 days under the dynamic perfusion condition of a mixed culture medium to obtain a three-dimensional gastric cancer organ model containing a capillary network; the method is expected to be used for rapid, low-cost and high-throughput screening of chemotherapy or anti-angiogenesis drugs and accurate prediction of individual curative effects of patients.

The invention relates to the technical field of microbial culture, and provides a culture device for agricultural microbial agent production and a use method, the culture device comprises a culture box, and the top of the culture box is hermetically and detachably provided with a box cover. According to the device, the air pump is controlled by the controller to be started, so that the air pressure in the closed space is increased, the air passes through the non-woven fabric and the polymer microporous membrane, more tiny bubbles can be generated under the action of the polymer microporous membrane, a nutrient solution flows and is mixed, and the stirring effect on the nutrient solution is achieved; a nutrient solution in the incubator flows into the culture dish through the liquid discharge pipe, so that the stirring of the nutrient solution and the injection of the nutrient solution into the culture dish to be mixed with a microbial inoculum can be completed without using more structural components, the operation is simple, and the condition of pollution caused by improper cleaning is avoided; therefore, the subsequent microbial agent culture effect is improved.

The invention provides an automatic microbial sample inspection device and method, the device comprises a base, a support column vertically arranged on the base, a sliding sleeve slidably arranged on the support column and a first driving assembly used for driving the sliding sleeve to lift up and down, a rotating seat is rotatably arranged on the sliding sleeve, and a second driving assembly is arranged between the rotating seat and the sliding sleeve. A plurality of needle tubes which are uniformly distributed in the circumferential direction of the supporting column and are used for containing reagents are arranged on the rotating seat, a sample bottle which is vertically arranged on the base is arranged below one needle tube, a detection head is arranged below the sample bottle, and the detection head is connected with a control circuit board; a third driving assembly used for pushing a piston in a needle tube located over the sample bottle is further arranged on the supporting column, and the first driving assembly, the second driving assembly and the third driving assembly are connected with the control circuit board. Due to the fact that the needle tubes are arranged, and the reagents contained in the needle tubes are different, the multiple reagents can be injected into the sample bottles through the device respectively, and diversity of inspection functions is achieved.

The invention provides a low-carbon modular artificial wetland system and a carbon sink collaborative purification method thereof, and relates to the technical field of artificial wetlands, the low-carbon modular artificial wetland system comprises a modular box body, and a water inlet layer, a carbon sink layer and a purification layer which are sequentially arranged in the modular box body from top to bottom; the water inlet layer is used for filtering suspended matters through coarse sand; the carbon sequestration layer is used for adsorbing carbon dioxide through biochar and carbon sequestration microorganisms, and meanwhile, the carbon sequestration capacity is enhanced through the plant-microorganism synergistic effect; the purification layer is used for purifying a water body through an iron-based phosphorus removal material and a zeolite denitrification filler. The technical problems of low-carbon material selection, carbon sink potential excavation, renewable energy source coupling and greenhouse gas emission inhibition of an existing constructed wetland system are solved, the technical effects of efficient sewage purification and carbon emission reduction are achieved, and a carbon emission reduction closed loop of the whole life cycle is achieved.

The invention discloses a method and a device for quantitatively detecting active microorganisms in a solid / semi-solid sample, and belongs to the field of microorganism detection. According to the method, detection is carried out based on a PMA-qPCR method, influence factors of the stability of an experimental result are analyzed, and a stability analysis model is constructed; the stability analysis model is shown in the specification; wherein S is a comprehensive stability score, and the higher the S value is, the better the stability of the experiment result is; the weight coefficient is the weight coefficient of the ith influence factor; the performance function is the performance function of the ith influence factor; the influence factors comprise sample suspension dilution ratio, PMA dye concentration, dye incubation time, blue light irradiation time, blue light irradiation area and impurity particle size. The method can be used for simultaneously detecting the activity of various microorganisms in a solid / semi-solid sample, the accuracy is high, the experimental period is short, and the quantitative result has important guiding significance.

The application discloses a pollution-preventing detection device, method and application for rapid detection of nucleic acid amplification, which comprises a detection part and a mixed reaction cabin, and the mixed reaction cabin comprises a reaction cabin main body and a reaction cabin cover which is in sealed connection with the reaction cabin main body and can be detached; a channel for connecting a hybridization liquid containing part and a mixed reaction part is arranged between the reaction cabin cover and the reaction cabin main body; the detection part comprises a detection inner core and a detection box body containing the detection inner core; a transparent observation window is arranged on the detection box body and corresponds to the reaction area position of the detection inner core; a flow guide device matched with the mixed reaction cabin is further arranged on the detection box body; the flow guide device comprises a flow guide pipe and a flow guide part; one end of the flow guide part is connected with the flow guide pipe, and the other end is connected with the detection inner core; the hybridization liquid and the sample mixed liquid are guided to the detection inner core; and the detection inner core displays the final detection result in the area corresponding to the transparent observation window. The application realizes that the liquid in the reaction tube flows out to the designated detection card in a completely closed environment, and has the advantages of good sealing effect and prevention of primer aerosol pollution.

The utility model discloses a tissue single cell constant-temperature grinder which comprises a base, a test tube support is arranged on the base, at least one test tube groove used for placing test tubes is arranged on the test tube support, a top support is arranged above the test tube support, and grinding rods are arranged at the positions, above the test tube grooves, of the top support. The top support is provided with a driver used for driving the grinding rods, and the base is provided with a lifting mechanism used for driving the top support to ascend or descend, and is characterized in that a heating water tank is arranged on the base, the top of the heating water tank is provided with insertion grooves below the test tube grooves, the bottoms of the test tubes are located in the corresponding insertion grooves, and the test tubes are arranged in the insertion grooves. A temporary storage groove is formed in the position, close to the front side of the heating water tank, of the inserting groove. The utility model provides a tissue single cell constant-temperature grinder which can automatically grind tissue cells, so that the grinding stability is good, the efficiency is high, and meanwhile, the temperature in the tissue cell grinding process can be ensured.

The invention relates to the technical field of shellfish breeding, in particular to a rapid microalgae breeding method for shellfish breeding based on a photobioreactor and a cement pool. The method comprises the following steps: firstly, optimizing a photobioreactor structure and an illumination layout, controlling a culture environment by matching with a multi-parameter regulation and control system, breeding microalgae seeds with high resistance and high carbon sequestration capacity, culturing to a preset density, and partially harvesting and inoculating into a cement pond, after partial harvest, the photobioreactor realizes continuous culture by supplementing disinfected seawater and nutritive salt; the harvested high-density microalgae are inoculated into a cement pond, nutritive salt is supplemented in a doubled mode, air and carbon dioxide mixed gas is used for continuous aerated culture, and the microalgae are promoted to rapidly reach the index growth period and used for being directly fed to shellfish larvae; according to the rapid microalgae breeding method for shellfish seedling culture, the yield and quality of microalgae are both considered, the success rate and productivity of aquatic seedling culture are effectively improved, the production cost and the labor intensity of workers are reduced, and the method is suitable for large-scale popularization.

According to the nucleic acid extraction and detection device and the use method, by forming a plurality of communicating holes and taking a rotary valve as a communicating medium, communication among functional chambers is achieved, then sample transfer is achieved, the whole transfer process is completed in an internally-closed environment, sample pollution is not caused, and the detection efficiency is improved. The rotating cover and the rotating valve rotate synchronously, and the rotating cover is also closed when the rotating valve is closed, so that the sealing performance of the cavity in the uniform mixing and reaction process is ensured, and the stability of a pre-stored reagent and the safety of the reaction process are ensured; the sample transfer step is reduced, the pollution risk is reduced, the whole process is completed only through manual operation without a power supply or other equipment, and the method can be used for household self-inspection or in areas with limited resources or even in extreme environments without power supply.

A system includes a flow cell and a fluidics system under pressure causing sheath and sample biological fluids to flow. The fluidics system includes a gas bubble remover to remove and eliminate gas bubbles in the sheath fluid. The flow cell receives sheath fluid from the fluidics system, wherein the sample biological fluid flows with cells or particles through the flow cell to be surrounded by the sheath fluid. A deflection chamber under the flow cell receives drops of the sample biological fluid and sheath fluid out of the flow cell to selectively deflect one or more of the drops along one or more deflection paths. A droplet deposition unit system in communication with the deflection chamber receives the selectively deflected drops in the stream of the sample biological fluid with the one or more biological cells or particles into one or more containers.

The invention discloses a micro-fluidic chip, extraction equipment and an exosome extraction method, which can be used for an enzymatic detection device. Wherein the micro-fluidic chip comprises a chip base body with a rotation center, the chip base body is provided with a sample introduction unit, a reagent unit, a filtering unit, a capturing unit, a waste liquid bin, a collecting unit and a switching structure, and the filtering unit is arranged on the downstream of the sample introduction unit in the centrifugal direction, communicates with the sample introduction unit and is used for filtering impurities in a sample; the capturing unit is arranged on the downstream of the filtering unit in the centrifugal direction and communicated with the filtering unit, the capturing unit is communicated with the reagent unit and located on the downstream of the reagent unit, and the capturing unit is used for capturing or releasing the extracted target object; the waste liquid bin and the collecting unit are arranged on the downstream of the capturing unit in the centrifugal direction, and the waste liquid bin communicates with the capturing unit through a communicating structure; the switching structure is arranged in the communication structure, the switching structure can selectively block the communication structure, and the switching structure can enable the capturing unit to be communicated with the collecting unit.

The invention relates to the technical field of microfluidics, in particular to a high-throughput and high-efficiency cell extrusion microfluidic system and a particle introduction method. And a closed-loop control system integrating real-time cell deformation sensing, dynamic parameter decision and physical extrusion and alternating electric field cooperative application is constructed. The chip is provided with an inlet shunting device, a micro-channel and a channel structure of a microelectrode. The method comprises the steps of real-time deformation image acquisition and analysis and dynamic extrusion parameter decision and cooperative application. The invention provides a high-flux and high-efficiency cell extrusion microfluidic system and a particle introduction method, and aims to solve the problems that the drug loading efficiency and the survival rate are difficult to consider due to heterogeneity in the existing cell extrusion technology and a physical extrusion and electroporation coordinated regulation strategy is lacked, so that personalized precise treatment of cells is realized, and the cell quality is improved. The drug loading efficiency, the cell survival rate and the high throughput are obviously improved.

A system and method for automatically digesting, culturing and growing a biopsy. The digester is a closed fluid transfer system, including disposable and durable parts, that receives a biopsy, washes the biopsy tissue, digests the tissue to separate the cells from each other, and seeds the cells in a culture flask. In one use, the system receives cells from a patient, expands and multiplies them, and returns them to the patient. A controller coordinates the motion of a plurality of pumps and the status of a plurality of valves, the pumps and the plurality of valves moving the fluid throughout the system. A sensor system assists the controller in volume and flow rate control of the fluids in the system, and in controlling the position of a tube providing air and fluids to a biopsy vial that includes the biopsy.

The invention relates to a temperature control method and device and nucleic acid amplification detection equipment. The method comprises the following steps: when a main heating element reaches a target working temperature and the temperature is stable, obtaining a current combination corresponding to each auxiliary heating element according to the target working temperature and a preset corresponding relation; wherein the preset corresponding relation is the relation between the temperature and the current of the auxiliary heating piece meeting the temperature uniformity requirement; each auxiliary heating element is arranged corresponding to each temperature compensation area; and according to the current combination, an auxiliary heating element is driven to carry out temperature compensation adjustment on the temperature compensation area. The temperature uniformity can be realized, and the temperature accuracy is ensured.

According to the device and the reagent for extracting the pathogen, the pathogen product separated from the uncultured sample extracted by using the device and the reagent can be subjected to a drug sensitivity test after simple culture and bacteria collection treatment, so that the culture process is shortened compared with pathogen diagnosis and identification of a clinical infected sample, and a drug sensitivity result can be obtained more quickly; and a more timely diagnosis and treatment reference is provided for clinicians.

The invention relates to a microfluidic nucleic acid detection device, and relates to the field of detection. Comprising a bottom plate, a chip inlet and outlet module erected on one side above the bottom plate, a chip table erected on the chip inlet and outlet module and used for bearing a micro-fluidic chip with a liquid bag, a displacement module erected above the chip table, and a crushing module erected on the displacement module and used for squeezing liquid in the liquid bag into the micro-fluidic chip, the liquid transferring module is erected on one side of the crushing module and is used for transferring liquid in the micro-fluidic chip to flow; the pneumatic module is erected above the front end of the bottom plate and is used for controlling a flow channel of the micro-fluidic chip to be opened and closed; the heating module is erected on the other side above the bottom plate and is used for locally heating the micro-fluidic chip and automatically detecting the temperature; and the fluorescent module is used for acquiring a fluorescent signal of the micro-fluidic chip. The micro-fluidic chip detection device has the advantages that different types of micro-fluidic chips can be rapidly detected, and an accurate result can be obtained.

A system for automated extraction of biological samples from a set of biological samples has a small footprint and minimal movement of consumable sets, thereby reducing potential contamination during the extraction process. The extraction system includes a set of reaction tubes, a storage unit for storing a set of consumables, a sample preparation unit, a sample extraction unit, a waste unit, a plurality of robots for moving the tubes, samples, and cassettes, and a programmable control system programmed to process the samples in a serial mode in which a series of samples are processed sequentially in time one after another, thereby maintaining a continuous mode. Thus, a fixed processing turnaround time for each sample is maintained regardless of when the sample starts processing.

The present invention relates to the technical field of medical instruments, and relates to the technical field of small amplification devices, especially portable amplification devices. Disclosed are a pressing and sealing apparatus for a POCT kit, and a molecular diagnostic device and operation method. The pressing and sealing apparatus for a POCT kit comprises a movable bracket, wherein a transmission assembly can drive the movable bracket to enable a sealing portion to seal a reaction chamber of the POCT kit when the movable bracket moves in a first direction; and when the transmission assembly drives the movable bracket to move in a second direction, a pressing portion comes into contact with the reaction chamber of the POCT kit to enable the reaction chamber of the POCT kit to tightly fit with a heat source. In the molecular diagnostic device including the pressing and sealing apparatus, a valve control portion and a clamping device share a second drive motor, a hot-melt bonding portion of the sealing portion and a detection and acquisition unit of the pressing portion share a first drive motor, a gas source drive part and a clamping apparatus cooperate with each other to drive liquid to flow through chambers of the kit, thereby achieving high integration, few drive parts, reliable operation, high detection accuracy, and small contamination.

The invention provides a micro-fluidic chip capable of culturing oral squamous cell carcinoma organoid and a preparation method thereof, and relates to the technical field of micro-fluidic chips, the chip comprises a four-layer structure: the first layer is a closed layer, the second layer is a culture solution / drug pool layer (containing a concentration gradient injection channel and a slow flow channel), the third layer is a matrix culture pool layer, and the fourth layer is a matrix culture pool layer. The fourth layer is a matrigel inflow layer (containing an S-shaped flow channel); the second layer and the third layer are integrally formed, and all the layers are connected through oxygen plasma welding; the problems that in the prior art, matrigel is prone to shifting, drug gradient regulation and control is poor, operation is tedious, and adaptability is insufficient are solved, the matrigel and organoids can be stably fixed, the drug concentration gradient can be precisely regulated and controlled, the nutrition supply efficiency is enhanced, the structural integration level and operation convenience are improved, and the application prospect is wide. The method is suitable for oral squamous cell carcinoma organoid culture, drug screening and tumor mechanism research.

The invention discloses an intelligent liquid supplementing and sampling system and method of a microorganism sampler and an unmanned flight system. A control module controls a sampling liquid pump to inject sampling liquid in a sampling liquid bottle into a wet-wall cyclone type sampler by a preset liquid injection amount and suspends the sampling liquid pump; the control module controls the fan to inject air into the wet-wall cyclone sampler for sampling, and adjusts the rotating speed of the fan in real time according to the flow detected by the sampling flow sensor, so that the sampling flow is maintained in a preset flow range; in the sampling process, the temperature sensor is used for detecting the temperature of the current air environment in real time per second, the humidity sensor is used for synchronously detecting the humidity of the current air environment in real time per second, and the control module is used for substituting the current temperature and the current humidity into a one-second sampling liquid evaporation capacity fitting function. And calculating the evaporation capacity of the sampling liquid in one second in the current temperature and humidity environment, controlling the working liquid supplementing time of the sampling liquid pump, and injecting the sampling liquid into the wet-wall cyclone type sampler to realize intelligent real-time liquid supplementing.

The present application provides a microfluidic device, comprising at least one microfluidic channel unit, each microfluidic channel unit comprising a microfluidic channel configured to contain a fluid and comprising a plurality of independent compartments. Each microfluidic channel unit comprises a first side wall and a second side wall opposite and spaced apart from the first side wall, a plurality of first extensions formed on the first side wall extending toward the second side wall without abutting the second side wall, a plurality of second extensions formed on the second side wall extending toward the first side wall without abutting the first side wall, the plurality of first extensions and the plurality of second extensions being alternately arranged in the microfluidic channel. A compartment is formed in at least one first extension or at least one second extension. The compartment has an opening positioned such that the fluid does not directly flow into each compartment. The microfluidic device of the present application has a stable isolated environment to facilitate various reactions and prevent micro-objects in the compartments from escaping.

The invention discloses a single-shaft adjustable mechanical stretching device for organoids or cells and a stretching culture method. A fixed support and a guide rail are arranged on a fixed base through a guide rail lifting seat, and a sliding block above the guide rail is connected with a stretching movement end through an adapter seat; the stretching moving end and the stretching fixed end are arranged at an interval, a lead screw of a stepping motor on the fixed support is fixedly connected to a coupler, and the coupler is fixedly connected with an adapter through a blocking block; handles on the two sides of the culture chamber are fixed to a stretching fixing base and a stretching fixing end respectively, a stepping motor is started, a lead screw of the stepping motor drives an adapting base to move longitudinally when moving, the longitudinal movement of the adapting base drives a sliding block to move on a guide rail, and then a stretching movement end is made to be far away from / close to the corresponding stretching fixing end; according to the invention, accurate and controllable tensile mechanical stimulation is carried out on different forms of organoids or cells through the replaceable culture chamber and the accurately controllable stepping motor, so that the tissue maturity / calcification degree is obviously changed or the cell differentiation process is adjusted.

The present disclosure relates to systems, devices, and methods for automated fluid transfer. In an embodiment, the present disclosure relates to a system for fluid transfer between a fluid device and a cartridge for cell processing. The system comprises a first portion comprising an instrument head coupled to a gantry, the instrument head comprising a pneumatic actuator, a peristaltic pump, and a gripping feature for receiving the fluid device. The peristaltic pump may be configured to engage compressible fluidic tubing of a fluid pump module of the fluid device to transfer the fluid between the fluid device and the cartridge. The system further comprises a second portion comprising a docking station for receiving the cartridge. In some variations, movement of the pneumatic actuator relative to the fluid pump module causes occlusion of the compressible fluidic tubing of the fluid pump module by the peristaltic pump.