13 results about "Sequencing by synthesis" patented technology

The invention is directed to methods for massively parallel template-free enzymatic synthesis of a plurality of different polynucleotides of predetermined sequences. In one aspect, methods of the invention employ large scale arrays of reaction sites each associated with at least one working electrode for controlling deprotection and deblocking steps at predetermined user selected sites. In another aspect, the invention provides template-free enzymatic synthesis with proofreading, wherein completed polynucleotides at predetermined reaction sites are sequenced using a sequencing by synthesis technique, particularly employing electrochemically labile blocking groups.

Embodiments of the present disclosure relate to methods and kits for sequencing by synthesis utilizing orthogonal or biorthogonal chemical reactions between unlabeled nucleotides and multi-dye labeled post incorporation labeling reagents containing water-soluble polymer scaffolds.

Embodiments of the present disclosure relate to multi-dye labeled polyphosphodiester scaffolds, as well as methods and kits for sequencing by synthesis utilizing such multi-dye labeled, scaffolds as post-incorporation labeling reagents.

Embodiments of the present disclosure relate to nucleotide molecules having an enzymatically cleavable self-immolative linker group. Also provided herein are methods and kits for sequencing applications using such nucleotides.

The present application relates to the technical field of organic compound reagent, specifically relates to a silicon-based rhodamine compound and a preparation method thereof, fluorescent modified nucleotide and kit, wherein the fluorescent modified nucleotide can be applied to the nucleic acid sequencing reaction of sequencing by synthesis. The present application creatively improves -COR 11 The connection structure between the fluorescent compound dye core structure adopts a heteroalkyl structure containing dioxygen -O-(CH2) m -O-(CH2) n -COR 11 As the connection structure, the connecting group -COR The silicon-based rhodamine compound is connected to the fluorescent compound core structure, and the fluorescence stability, fluorescence intensity and incorporation efficiency of the modified nucleotide formed by the silicon-based rhodamine compound as a modified molecule used in the nucleic acid sequencing reaction are improved.

A method for real-time sequence analysis of DNA fragments includes providing a DNA fragments sample and connecting first and second adapter oligonucleotides to 5′ and 3′ ends of a DNA strand of the DNA fragments, respectively. A first adapter oligonucleotide includes from 5′ to 3′ a) a first flow cell binding sequence, b) a read 1 sequencing primer site, c) optionally a random sequence, and d) a sample-specific barcoding sequence. A second adapter oligonucleotide includes from 5′ to 3′ d) a sequence complementary to the sample-specific barcoding sequence, c) optionally a sequence complementary to the random sequence, b) a read 2 sequencing primer site that might be complementary to the read 1 sequencing primer site, and a) a second flow cell binding sequence. First and second adapter oligonucleotides of one kind have complementary barcoding sequences. A sequencing by synthesis process is used for sequencing the connected adapter oligonucleotides.

The present invention provides compositions and methods for nucleic acid sequencing and other applications. In sequencing by synthesis, an unlabeled reversible terminator is incorporated in each cycle by a polymerase and then labeled after incorporation by binding a directly or indirectly labeled antibody or other affinity reagent to the reversible terminator.

The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, recombinant polymerases and biologically active fragments thereof are provided that allow for nucleic acid amplification. In some aspects, the disclosure provides recombinant polymerases that yield lower systematic error rates and / or improved accuracy, when used in sequencing by synthesis reactions as compared to a control polymerase. In one aspect, the disclosure relates to recombinant polymerases useful for nucleic acid sequencing, genotyping, copy number variation analysis, paired-end sequencing and other forms of genetic analysis. In another aspect, the recombinant polymerases are useful for the amplification of nucleic acid templates during PCR, emPCR, isothermal amplification, recombinase polymerase amplification, rolling circle amplification, strand displacement amplification and proximity ligation amplification. In some aspects, the disclosure relates to recombinant polymerases useful for the generation of nucleic acid libraries and / or nucleic acid templates.

Disclosed herein are highly accurate approaches for single molecule electronic nanopore-based SBS.

Systems and methods for nucleic acid sequencing are provided. Polynucleotide strands to be sequenced are immobilized to a surface, clonally amplified and primed for polynucleotide synthesis. Nucleotides modified with cleavable electroactive labels at the 3'-OH of the sugar ring are provided and are incorporated into the growing strand during synthesis. The labels of nucleotides that are successfully incorporated into the growing polynucleotide strand are cleaved via application of a stimulus after incorporation and are detected by an electronic nanosensor. The systems and methods described herein combine the high accuracy of SbS methods combined with the scalability and speed of semiconductor-based electrical detection mechanisms.

Systems and methods for nucleic acid sequencing are provided. Polynucleotide strands to be sequenced are immobilized to a surface, clonally amplified and primed for polynucleotide synthesis. Nucleotides modified with cleavable electroactive labels at the 3′-OH of the sugar ring are provided and are incorporated into the growing strand during synthesis. The labels of nucleotides that are successfully incorporated into the growing polynucleotide strand are cleaved via application of a stimulus after incorporation and are detected by an electronic nanosensor. The systems and methods described herein combine the high accuracy of SbS methods combined with the scalability and speed of semiconductor-based electrical detection mechanisms.