5 results about "Sequencing by synthesis" patented technology

Embodiments of the present disclosure relate to multi-dye labeled polyphosphodiester scaffolds, as well as methods and kits for sequencing by synthesis utilizing such multi-dye labeled, scaffolds as post-incorporation labeling reagents.

The present application relates to the technical field of organic compound reagent, specifically relates to a silicon-based rhodamine compound and a preparation method thereof, fluorescent modified nucleotide and kit, wherein the fluorescent modified nucleotide can be applied to the nucleic acid sequencing reaction of sequencing by synthesis. The present application creatively improves -COR 11 The connection structure between the fluorescent compound dye core structure adopts a heteroalkyl structure containing dioxygen -O-(CH2) m -O-(CH2) n -COR 11 As the connection structure, the connecting group -COR The silicon-based rhodamine compound is connected to the fluorescent compound core structure, and the fluorescence stability, fluorescence intensity and incorporation efficiency of the modified nucleotide formed by the silicon-based rhodamine compound as a modified molecule used in the nucleic acid sequencing reaction are improved.

A method for real-time sequence analysis of DNA fragments includes providing a DNA fragments sample and connecting first and second adapter oligonucleotides to 5′ and 3′ ends of a DNA strand of the DNA fragments, respectively. A first adapter oligonucleotide includes from 5′ to 3′ a) a first flow cell binding sequence, b) a read 1 sequencing primer site, c) optionally a random sequence, and d) a sample-specific barcoding sequence. A second adapter oligonucleotide includes from 5′ to 3′ d) a sequence complementary to the sample-specific barcoding sequence, c) optionally a sequence complementary to the random sequence, b) a read 2 sequencing primer site that might be complementary to the read 1 sequencing primer site, and a) a second flow cell binding sequence. First and second adapter oligonucleotides of one kind have complementary barcoding sequences. A sequencing by synthesis process is used for sequencing the connected adapter oligonucleotides.