698 results about "Flow cell" patented technology

The present disclosure relates to systems, devices, and methods for automated cell sorting within a cell processing system. In an embodiment, the present disclosure relates to an automated cell sorting system comprising a cartridge having a cell sorting module, where the cell sorting module comprises a flow cell, and an instrument within a bay of a cell processing workcell, where the instrument comprises a magnetic array couplable to the flow cell, each of the magnets within the magnetic array having a width of w and being spaced apart by between about w / 3 to about ¾w, and wherein the flow cell has a height of between about 1 / 12w to about ⅛w.

The invention focuses on the field of online turbidity analysis of industrial circulating water, and provides an innovative analysis method. According to the method, a two-stage defoaming and dynamic calibration collaborative strategy is adopted to solve the problem that a traditional online turbidity meter is easily interfered by bubbles and temperature fluctuation in a low-turbidity environment, so that the measurement error is large. Primary defoaming is completed by arranging a porous bubble adsorption layer in a flow cell, and secondary defoaming is realized by virtue of centrifugal force of rotating blades of a centrifugal degassing unit; double light paths (infrared light and visible light) are used for cross validation of turbidity signals, and the light path sensitivity is dynamically adjusted according to the water turbidity; and constructing a temperature-turbidity correlation model to correct the temperature drift influence in real time, and constructing a historical data trend library to predict the turbidity change. Through application of a chemical plant circulating water system, the error in a low-turbidity scene is remarkably reduced, and the stability is greatly improved.

The embodiment of the invention provides a concentration measuring device and a concentration measuring method. The concentration measuring device comprises a light source module, a plurality of flow cells, a light splitting and on-off module and a processing and control module. The light splitting and on-off module is used for splitting light emitted by the light source module into multiple paths of incident light and transmitting the incident light to the flow cells, and meanwhile one of the characteristic light output by the flow cells can be selected to be transmitted to the processing and control module. And the processing and control module receives the concentration value of the flow cell corresponding to the characteristic light output. According to the concentration measuring device provided by the embodiment of the invention, by arranging the light splitting and on-off module, it is ensured that only the characteristic light output by one flow cell can be transmitted to the processing and control module at any moment, and the characteristic light data of the flow cell at each process point can be sequentially obtained through light path switching, so that monitoring of the concentration of a plurality of process points is realized; the equipment cost can be saved, the system structure is simplified, the maintenance amount is reduced, and the operation difficulty is reduced.

A system includes a flow cell and a fluidics system under pressure causing sheath and sample biological fluids to flow. The fluidics system includes a gas bubble remover to remove and eliminate gas bubbles in the sheath fluid. The flow cell receives sheath fluid from the fluidics system, wherein the sample biological fluid flows with cells or particles through the flow cell to be surrounded by the sheath fluid. A deflection chamber under the flow cell receives drops of the sample biological fluid and sheath fluid out of the flow cell to selectively deflect one or more of the drops along one or more deflection paths. A droplet deposition unit system in communication with the deflection chamber receives the selectively deflected drops in the stream of the sample biological fluid with the one or more biological cells or particles into one or more containers.

The invention discloses a dynamic leaching-spectrophotometric detection method for the release rate of nutrients in a slow-release fertilizer, and relates to the field of agrochemical analys.The detection method comprises the following steps that S1, a slow-release fertilizer sample is placed in a leaching column to be leached, and leachate is collected; s2, sequentially adding a masking agent solution and a chromogenic reagent into the leachate, and carrying out chromogenic reaction; s3, pumping the solution into a double-optical-path flow cell, and measuring absorbance values under a target nutrient characteristic absorption wavelength and a background interference characteristic wavelength; s4, calculating the corrected net absorbance; and S5, calculating the nutrient concentration, and drawing a nutrient release kinetic curve. By adopting a dual-wavelength synchronous detection and real-time dynamic subtraction correction algorithm, the method can eliminate system errors of time-varying background chromaticity, so that accurate and reliable absorbance readings can be obtained at the initial stage of nutrient release even if the nutrient concentration in the leachate is very low, thereby ensuring the authenticity of the initial stage of a release curve and improving the accuracy of nutrient release. And initial release kinetic parameters are accurately captured.

Systems and methods for measuring water concentration in a liquid medium include a flow cell configured to fluidly couple to a pipe; a light source coupled to a first side of the flow cell; an infrared camera coupled to a second side of the flow cell opposite the first side; and at least one processor and a memory storing instructions that when executed by the at least one processor cause the at least one processor to perform operations. The operations include acquiring one or more images from the infrared camera; detecting water droplets in the one or more images using a trained machine learning model; and determining a concentration of water in the liquid medium based on the detected water droplets.

The present disclosure provides flow cell devices, systems, and methods for facilitating and performing DNA sequencing analysis with reduced system complexity and cost, significant cost of goods (COGS) savings and reduced contamination levels.

Methods and systems for analysis of image data generated from various reference points. Particularly, the methods and systems provided are useful for real time analysis of image and sequence data generated during DNA sequencing methodologies.

A method and instrument for determining optical density of a solution is disclosed. A flow cell 1 having at least three light paths (4a, 4b, 4c) is provided (100), wherein each light path has a respective predetermined path length, l. Absorbance readings are taken (400), A, of the solution at the at least three light paths (4a, 4b, 4c). For each pair of light paths, a slope, αc, is calculated (500) by dividing a difference in absorbance reading, ΔA, with a difference in path length, Δl. The calculated slopes, αc, are compared (600), and a) if the calculated slopes, αc, are the same, the slope is used for determining (700) optical density of the solution, or b) if the calculated slopes, αc, are not the same, the steepest slope of the calculated slopes is used for determining (701a) optical density of the solution, or the slope of the calculated slopes being in the range of an absorbance reading of 0.01 to 2 is used for determining (701b) optical density of the solution.

The invention discloses a surface plasmon resonance imaging system for expanding a dynamic range through a multi-mode optical path, and relates to the technical field of microscopic imaging, a laser outputs a monochromatic and linearly polarized continuous wave beam, a first dynamic beam deflection device performs dynamic angle deflection adjustment on the continuous wave beam, a prism is used for providing a total reflection interface, and a second dynamic beam deflection device is used for performing dynamic angle deflection adjustment on the continuous wave beam. Exciting an evanescent wave; a surface plasma resonance effect is generated through interaction of the SPR detection chip and evanescent waves, and sample characteristics are detected; the flow cell provides a sample flow channel, so that a sample is in full contact with the surface of the SPR detection chip; the second dynamic light beam deflection device cooperatively adjusts the propagation direction of reflected light on the surface of the SPR detection chip, and the imaging detection device captures a high-spatial-resolution SPR image formed by the reflected light and transmits the image to the upper computer in real time for processing and analysis. According to the invention, the change of the resonance angle can be tracked in real time, and high-sensitivity and high-resolution monitoring of the dynamic process of biomolecule interaction is realized.

The utility model provides an electrode device for detecting Zeta potential of a high-concentration sample by electrophoresis light scattering, which comprises a laser, a high-concentration sample electrode and a photoelectric detector, an incident laser beam emitted by the laser is incident in a manner of being vertical to the outer vertical surface of a micro-flow cell, and an optical fiber head of the photoelectric detector is positioned in a direction forming an angle of 12 degrees with the laser beam; the high-concentration sample electrode adopts a flow-through design, and the minimum optical path of a micro flow cell is 1.5 mm; a sample can be added into the micro-flow cell through the silicone tube. According to the utility model, the Zeta potential test requirement of a high-concentration sample can be met, the minimum sample quantity is 57 microliters, the electrode cost is low, the electrode can be replaced, and the Zeta potential titration test can be carried out by being connected with an automatic titrator.

The invention relates to the technical field of intelligent monitoring, and discloses a full-automatic sediment monitoring method and system.The method comprises the steps that a water sampling and distributing unit samples a target water area based on real-time sampling control parameters and conveys a sampled first water sample to a flow cell unit based on sampling control parameters; the flow cell unit simulates and forms a water sample monitoring water area environment matched with the target water area condition; the detection unit detects the water sample in the flow cell unit through a target detection method based on the detection control parameters to obtain a sediment detection result at least containing the sediment content; the reserved sample unit is used for extracting a reserved sample water sample for verifying the sediment detection result from the flow cell unit based on the reserved sample control parameters, and storing the reserved sample water sample and corresponding multi-dimensional water sample parameters. Therefore, the sediment condition of the related water area can be automatically and intelligently tested based on the full-automatic sediment monitoring system, and the testing efficiency and testing accuracy of the sediment condition can be improved.

A resealable fluidic device for conducting assays of a biological sample comprises a frame having a sample opening; and a cover slide configured to be received in the frame; wherein the frame is configured to receive a base slide on which a biological sample is configured to be disposed; wherein the cover slide and the base slide are configured to form a flow cell chamber housing the biological sample, and the flow cell chamber is configured to be aligned with the sample opening when the frame, the cover slide, and the based slide are assembled.

Provided herein are apparatuses and methods of releasing or converting a gas. In some embodiments, an apparatus may include a gas flow cell having an inlet and an outlet for flowing a gas through said gas flow cell; a structured material within said gas flow cell, wherein the structured material has an electrical conductivity selected from the range of 3×10−15 S / m to 6.3×107 S / m; and a plasma source integrated with said gas flow cell, the plasma source being configured to generate a plasma within a portion of the gas flow cell, the plasma source comprising: a first electrode and a second electrode within said gas flow cell; and a power source configured to provide voltage to the first and second electrodes.

The invention discloses a fluorescence detector based on an off-axis parabolic mirror, and belongs to the technical field of liquid chromatographic analysis, the fluorescence detector comprises a light source light path, an excitation monochromatic light path, a flow cell light path, a fluorescence collection light path, a fluorescence emission light path and a detector which are arranged in sequence, and the light source light path generates and converges ultraviolet-visible light; the converged ultraviolet-visible light is converted into monochromatic light with different wavelengths through the monochromatic light excitation path, the monochromatic light irradiates a to-be-detected sample in the flow cell light path to generate fluorescence, the fluorescence collection light path collects and converges the fluorescence, the converged fluorescence is divided into monochromatic fluorescence with different wavelengths through the fluorescence emission light path, and the monochromatic fluorescence is finally detected by the detector. The light source light path and the fluorescence collection light path both adopt off-axis parabolic reflectors to replace lenses, so that the influence of chromatic aberration is eliminated, and the utilization rate of optical energy is improved; the two off-axis parabolic reflectors are matched with each other, so that high spectral resolution is realized, and a simulation imaging light spot is less than 20 microns.

Disclosed herein are sequencing systems and sequencing methods for training neural networks and for utilizing the trained neural networks for sequencing analysis after acquiring flow cell images using the sequencing systems. The sequencing systems disclosed herein can include Field-Programmable Gate Array (FPGAs), artificial intelligence (AI) chips, or a combination thereof.

The invention discloses an activated sludge fluorescence online detection device based on a flow cell, and relates to the field of activated sludge biomass detection.The activated sludge fluorescence online detection device comprises a fluorescence spectrophotometer and a fluorescence spectrophotometer sample table arranged on the fluorescence spectrophotometer, and the fluorescence spectrophotometer is connected with a processor through a cable; the fluorescence spectrophotometer sample table is connected with a sample cup through a guide pipe, activated sludge is arranged in the sample cup, and a peristaltic pump is arranged on the guide pipe; the activated sludge continuously flows through the fluorescence spectrophotometer sample table through the guide pipe for three-dimensional fluorescence detection through the peristaltic pump, sludge sedimentation in a traditional cuvette is avoided, measurement is simple and convenient, and on-line real-time monitoring of the activated sludge biomass can be achieved.

A fluid delivery system and process for fluid delivery is provided that is useful in in flow-injection based sensing measurements, wherein samples from a sample rack are introduced into a flow cell where sensing measurements are taken. The system and process utilize at least two holding lines where each holding line can retain one of the samples prior to injection of the thus retained sample into a flow cell. The introduction of the samples from the sample racks to the holding lines is alternated and the injection of the samples from the holding lines to the flow cell are alternated so that one holding line is loaded with one of the samples simultaneously with another sample being injected from the another holding line to the flow cell.

Image data analysis, and particularly identifying cluster or polony locations for performing base-calling in a digital image of a flow cell during DNA sequencing is described. A method may include generating a first plurality of flow cell images of a cellular sample immobilized on a support by conducting one or more cycles of sequencing reactions. The cellular sample may include a plurality of concatemer molecules therewithin. For the first plurality of flow cell image, pixel intensities, and a respective color purity of each of the pixel intensities may be determined. A base calling template may include base calling locations based on the pixel intensities and the respective color purity of the pixel intensities. The base calling template may be for registering a second plurality of flow cell images of the support in one or more subsequent cycles of the one or more cycles.

Apparatuses and corresponding methods are provided for determining molecular properties of a molecule. To determine molecular properties of the molecule, a molecule library comprising the molecule is generated, a focused library comprising the molecule is generated by filtering the molecule library; the focused library is attached to a flow cell of the imaging instrument; and a fluid control module of the imaging instrument is controlled to incubate the flow cell with a fluid comprising labelled target molecules. A light source module and an imaging module of the imaging instrument are operated in a selected operational mode such that the light source module illuminates the flow cell and the imaging module captures light generated by fluorescence by the labelled target molecules within the flow cell. Image data is generated based on the captured light and the imaging data is analyzed to determine molecular properties of the at least one molecule.

A kit includes a flow cell, an extension mix, and an enzymatic methylation conversion mix. The flow cell includes a substrate having depressions separated by interstitial regions; first and second primers immobilized within each of the depressions; and first and second transposome complexes immobilized within each of the depressions and / or over each of the interstitial regions. The kit may be used for on-flow cell library preparation and methylation detection.

A particle measuring device includes a flow cell unit including a cell hollow portion in which a liquid sample flows, a mount unit including an accommodation hole accommodating at least a portion of the flow cell unit, and a resonance unit positioned behind the mount unit and coupled to the mount unit. The mount unit includes a mount body in which the accommodation hole is formed, and a passage hole depressed in an outer surface of the mount body and communicating with the accommodation hole. The resonance unit includes a resonance body behind the mount body and coupled to the mount body, and a cavity that is a space formed in the resonance body and communicates with the passage hole.

A flow nanocytometer for measuring extracellular vesicles (EVs) may include a flow cell for receiving a core stream carry at least one EV, at least one excitation source module for outputting a light beam to interrogate a portion of the core stream, a detection module for detecting the light beam, and a processor communicatively coupled to a memory, the at least one excitation source, and the detection module. The processor may execute a method including receiving a plurality of electronic signals from the detection module; detecting an intensity of a polycystic kidney disease (PKD) associated protein expressed by the EVs based on the plurality of electronic signals; determining a quantity of the EVs based on the plurality of electronic signals; and determining a severity or a prognosis of polycystic kidney disease in the subject, based on the intensity of the PKD associated protein and the quantity of the EVs.

The invention provides an optical instrument self-cleaning micro flow cell, and relates to the technical field of optical detection equipment, and the optical instrument self-cleaning micro flow cell comprises a self-cleaning analysis module which is used for analyzing a pollution degree index in a measurement cell, and determining whether to generate a self-cleaning signaling according to the pollution degree index; when it is determined that the self-cleaning signaling is generated, calculating a power adjustment value of the ultrasonic vibrator and a rotating speed adjustment value of the magnetic stirrer; and the control module is used for adjusting the power of the ultrasonic vibrator and the rotating speed of the magnetic stirrer according to the power adjustment value and the rotating speed adjustment value after receiving the self-cleaning signaling. An ultrasonic wave and magnetic force synergistic self-cleaning mode is adopted, a mechanical cleaning device is not needed, damage of mechanical cleaning to the structure and the sealing performance of the flow cell is avoided, the reliability of equipment is improved, the service life of the equipment is prolonged, targeted efficient cleaning is achieved through accurate pollution degree index calculation and self-cleaning parameter adjustment, and the service life of the equipment is prolonged. The cleaning effect can be guaranteed, and energy consumption can be reduced.

The present invention describes a gas volume and flow measurement device 1 comprising a flow cell compartment 2 with a definite and predefined 5 inner geometric volume, the flow cell compartment 2 having one gas accumulating end 3 and one lifting end 4 and having a pivoting element 5 enabling the flow cell compartment 2 to pivot upwards to release the gas contained therein and then downwards again to its initial position and operating by liquid displacement and according to the leverage effect, wherein said gas flow measurement device 1 also comprises a bubble counter unit 6.

An adapter for incorporation into an open well flow cell, the adapter including a body having an upper surface, a lower surface spaced from the upper surface defining a thickness profile therebetween, at least one side extending about a perimeter of the body, an inlet formed in the body extending through the thickness of the upper surface and the lower surface, at least one foot extending from the lower surface, each foot of the at least one foot extending a vertical distance away from the lower surface and a protrusion extending from the upper surface. The adaptor configured to be placed over, or onto, an open well flow cell to form an effectively closed, and reversible, flow cell. A variety of structural features are included to inhibit / prohibit gas bubbles from evaporating from the fluid reagent within the cell, and / or providing egress or venting of such bubbles.

The utility model discloses and provides an automatic backwashing pretreatment system for a water quality on-line instrument, which comprises a sample inlet, a washing water inlet, a normal-pressure drain outlet, an electric control box and a water quality on-line analyzer, the on-line water quality analyzer sends sampling signals to the electrical control box to control the on-off states of the first pneumatic three-way valve and the second pneumatic three-way valve, and the flushing state is maintained in a normal state, so that the workload of maintenance and cleaning is greatly reduced, algae generation in the system is greatly reduced, the cleanliness is improved, and the service life of the system is prolonged. And during sampling, the flushing state is closed, a sample enters the sampling flow cell after passing through the filter to be sampled and analyzed, automatic control is achieved, and time and worry are saved.

The invention belongs to the technical field of water environment detection, and particularly relates to a heavy metal concentration and water body flow velocity synchronous detection method for a dispersed drain outlet, which is used for synchronously detecting the heavy metal cadmium concentration and the water body flow velocity. The core of the method comprises a three-electrode sensing system consisting of a working electrode coated with a tungsten oxide film, a reference electrode and a counter electrode, and is assisted by an electrochemical workstation, a peristaltic pump, a flow cell and a signal processing module. The cadmium concentration is accurately measured through the square wave anodic stripping voltammetry, the flow velocity is synchronously detected through the open circuit potentiometry, dual-function detection is achieved through integration of the square wave anodic stripping voltammetry and the open circuit potentiometry, synchronous in-situ detection of the cadmium concentration and the water body flow velocity is achieved, and errors caused by measurement position differences are effectively eliminated. The result shows that the cadmium concentration and the dissolution peak current are in a linear relationship, and the water flow velocity logarithm and the open-circuit potential are in a linear relationship. The method designed by the invention is suitable for scattered and small sewage draining exit scenes, and effectively solves the problems of time consumption and equipment redundancy of traditional step-by-step detection. In an actual water sample test, the cadmium recovery rate is 80%-120%, the flow velocity measurement RSD is smaller than or equal to 5.0%, high reproducibility, stability and anti-interference performance are achieved, technical support is provided for real-time online evaluation of the total heavy metal emission amount, and the method has remarkable environmental monitoring application potential.

The present application relates to spectral technology, and specifically provides a nitrogen oxide analysis device and method, the analysis device comprising a first light source; further comprising: a flow cell having a main flow channel along its axial direction, an inlet being arranged adjacent to a first end of the flow cell, and an outlet being arranged adjacent to a second end; the first end, the inlet, the outlet and the second end are sequentially arranged in the axial direction of the flow cell; a first flow channel and a second flow channel are arranged in the body of the flow cell, and the gas sequentially passes through the inlet, the first flow channel and the second flow channel, enters the main flow channel and flows in the direction from the first end to the second end; a third flow channel and a fourth flow channel are arranged in the body of the flow cell, and the gas sequentially passes through the third flow channel, the fourth flow channel and the outlet in the second end; the flow directions of the gas in the third flow channel and the second flow channel are the same and opposite to the flow direction in the main flow channel. The present application has the advantages of accurate measurement results.

The present disclosure provides flow cell devices, systems, and methods for facilitating and performing DNA sequencing analysis with reduced system complexity and cost, significant cost of goods saving, and reduced contamination level. The sequencing systems described herein permit processing of multiple flow cells simultaneously, such that sequencing and imaging steps, or multiple sequencing methods, can be performed in parallel using a single sequencing system.