965 results about "Flow cell" patented technology

The present disclosure relates to systems, devices, and methods for automated cell sorting within a cell processing system. In an embodiment, the present disclosure relates to an automated cell sorting system comprising a cartridge having a cell sorting module, where the cell sorting module comprises a flow cell, and an instrument within a bay of a cell processing workcell, where the instrument comprises a magnetic array couplable to the flow cell, each of the magnets within the magnetic array having a width of w and being spaced apart by between about w / 3 to about 3 / 4w, and wherein the flow cell has a height of between about 1 / 12w to about 1 / 8w.

The present disclosure provides flow cell devices, systems, and methods for facilitating and performing DNA sequencing analysis with reduced system complexity and cost, significant cost of goods saving, and reduced contamination level. The sequencing systems described herein permit processing of multiple flow cells simultaneously, such that sequencing and imaging steps, or multiple sequencing methods, can be performed in parallel using a single sequencing system.

The present disclosure relates to systems, devices, and methods for automated cell sorting within a cell processing system. In an embodiment, the present disclosure relates to an automated cell sorting system comprising a cartridge having a cell sorting module, where the cell sorting module comprises a flow cell, and an instrument within a bay of a cell processing workcell, where the instrument comprises a magnetic array couplable to the flow cell, each of the magnets within the magnetic array having a width of w and being spaced apart by between about w / 3 to about ¾w, and wherein the flow cell has a height of between about 1 / 12w to about ⅛w.

The present disclosure relates to systems, devices, and methods for automated cell sorting within a cell processing system. In an embodiment, the present disclosure relates to an automated cell sorting system comprising a cartridge having a cell sorting module, where the cell sorting module comprises a flow cell, and an instrument within a bay of a cell processing workcell, where the instrument comprises a magnetic array couplable to the flow cell, each of the magnets within the magnetic array having a width of w and being spaced apart by between about w / 3 to about 3 / 4w, and wherein the flow cell has a height of between about 1 / 12w to about 1 / 8w.

Methods and systems for analysis of image data generated from various reference points. Particularly, the methods and systems provided are useful for real time analysis of image and sequence data generated during DNA sequencing methodologies.

The invention focuses on the field of online turbidity analysis of industrial circulating water, and provides an innovative analysis method. According to the method, a two-stage defoaming and dynamic calibration collaborative strategy is adopted to solve the problem that a traditional online turbidity meter is easily interfered by bubbles and temperature fluctuation in a low-turbidity environment, so that the measurement error is large. Primary defoaming is completed by arranging a porous bubble adsorption layer in a flow cell, and secondary defoaming is realized by virtue of centrifugal force of rotating blades of a centrifugal degassing unit; double light paths (infrared light and visible light) are used for cross validation of turbidity signals, and the light path sensitivity is dynamically adjusted according to the water turbidity; and constructing a temperature-turbidity correlation model to correct the temperature drift influence in real time, and constructing a historical data trend library to predict the turbidity change. Through application of a chemical plant circulating water system, the error in a low-turbidity scene is remarkably reduced, and the stability is greatly improved.

An integrated optical fluid interrogation module is provided. An integrated optical fluid interrogation module of interest comprises: a flow cell comprising an optically accessible flow channel for transporting particles in a flow stream; a linear fiber array integrated with the flow cell and including a plurality of non-circular core fibers, each non-circular core fiber configured to project excitation wavelength light onto a corresponding focus within the flow cell; and a plurality of fiber optic light transmitter couplers for operably attaching the plurality of light sources to the linear fiber array. Flow cytometers including the subject integrated optical fluid interrogation module and methods of using the same are also provided.

The embodiment of the invention provides a concentration measuring device and a concentration measuring method. The concentration measuring device comprises a light source module, a plurality of flow cells, a light splitting and on-off module and a processing and control module. The light splitting and on-off module is used for splitting light emitted by the light source module into multiple paths of incident light and transmitting the incident light to the flow cells, and meanwhile one of the characteristic light output by the flow cells can be selected to be transmitted to the processing and control module. And the processing and control module receives the concentration value of the flow cell corresponding to the characteristic light output. According to the concentration measuring device provided by the embodiment of the invention, by arranging the light splitting and on-off module, it is ensured that only the characteristic light output by one flow cell can be transmitted to the processing and control module at any moment, and the characteristic light data of the flow cell at each process point can be sequentially obtained through light path switching, so that monitoring of the concentration of a plurality of process points is realized; the equipment cost can be saved, the system structure is simplified, the maintenance amount is reduced, and the operation difficulty is reduced.

The invention provides methods and compositions, including, without limitation, algorithms, computer readable media, computer programs, apparatus, and systems for determining the identity of nucleic acids in nucleotide sequences using, for example, data obtained from sequencing by synthesis methods. A plurality of smaller flow cells is employed, each with a relatively small area to be imaged, in order to provide greater flexibility and efficiency.

A system includes a flow cell and a fluidics system under pressure causing sheath and sample biological fluids to flow. The fluidics system includes a gas bubble remover to remove and eliminate gas bubbles in the sheath fluid. The flow cell receives sheath fluid from the fluidics system, wherein the sample biological fluid flows with cells or particles through the flow cell to be surrounded by the sheath fluid. A deflection chamber under the flow cell receives drops of the sample biological fluid and sheath fluid out of the flow cell to selectively deflect one or more of the drops along one or more deflection paths. A droplet deposition unit system in communication with the deflection chamber receives the selectively deflected drops in the stream of the sample biological fluid with the one or more biological cells or particles into one or more containers.

A heat exchanger has a first inlet in fluid communication with a tank that contains a first chemical solution of a known concentration, and a second inlet in fluid communication with a conduit. The conduit is in fluid communication with a process tool and conveys a second chemical solution from the process tool to the second inlet. The heat exchanger receives a quantity of the first chemical solution via the first inlet and a quantity of the second chemical solution via the second inlet, and adjusts a temperature of the received quantity of the first chemical solution to bring the temperature of the received quantity of the first chemical solution toward a temperature of the received quantity of the second chemical solution. The temperature-adjusted first chemical solution can be selectively provided to an analytic flow cell for verification and / or calibration of the flow cell.

The invention discloses a dynamic leaching-spectrophotometric detection method for the release rate of nutrients in a slow-release fertilizer, and relates to the field of agrochemical analys.The detection method comprises the following steps that S1, a slow-release fertilizer sample is placed in a leaching column to be leached, and leachate is collected; s2, sequentially adding a masking agent solution and a chromogenic reagent into the leachate, and carrying out chromogenic reaction; s3, pumping the solution into a double-optical-path flow cell, and measuring absorbance values under a target nutrient characteristic absorption wavelength and a background interference characteristic wavelength; s4, calculating the corrected net absorbance; and S5, calculating the nutrient concentration, and drawing a nutrient release kinetic curve. By adopting a dual-wavelength synchronous detection and real-time dynamic subtraction correction algorithm, the method can eliminate system errors of time-varying background chromaticity, so that accurate and reliable absorbance readings can be obtained at the initial stage of nutrient release even if the nutrient concentration in the leachate is very low, thereby ensuring the authenticity of the initial stage of a release curve and improving the accuracy of nutrient release. And initial release kinetic parameters are accurately captured.

Systems and methods for measuring water concentration in a liquid medium include a flow cell configured to fluidly couple to a pipe; a light source coupled to a first side of the flow cell; an infrared camera coupled to a second side of the flow cell opposite the first side; and at least one processor and a memory storing instructions that when executed by the at least one processor cause the at least one processor to perform operations. The operations include acquiring one or more images from the infrared camera; detecting water droplets in the one or more images using a trained machine learning model; and determining a concentration of water in the liquid medium based on the detected water droplets.

The present disclosure provides flow cell devices, systems, and methods for facilitating and performing DNA sequencing analysis with reduced system complexity and cost, significant cost of goods (COGS) savings and reduced contamination levels.

Methods and systems for analysis of image data generated from various reference points. Particularly, the methods and systems provided are useful for real time analysis of image and sequence data generated during DNA sequencing methodologies.

The invention discloses a flow cell with a lubricating oil abrasive particle measuring function, and relates to the technical field of lubricating oil, the flow cell comprises a measuring main body, two pipelines, a measuring instrument and a light emitting instrument; a cavity is formed in the measuring main body; the cavity penetrates through two sides of the measuring main body to form two circulation ports; the pipelines are communicated with the cavity through the corresponding circulation openings; the pipeline has a structure with a round top and a square bottom; a first mounting opening and a second mounting opening are respectively formed in two side surfaces of the measuring main body; the measuring instrument is arranged on the measuring main body through the first mounting port to seal the first mounting port, and part of the measuring instrument is located in the cavity; and the light-emitting instrument is arranged on the measuring main body through the second mounting port to seal the second mounting port. According to the invention, the accuracy of the detection result can be ensured, the flowing circulation of the lubricating oil can be effectively improved, and the lubricating oil can be recycled conveniently.

A method and instrument for determining optical density of a solution is disclosed. A flow cell 1 having at least three light paths (4a, 4b, 4c) is provided (100), wherein each light path has a respective predetermined path length, l. Absorbance readings are taken (400), A, of the solution at the at least three light paths (4a, 4b, 4c). For each pair of light paths, a slope, αc, is calculated (500) by dividing a difference in absorbance reading, ΔA, with a difference in path length, Δl. The calculated slopes, αc, are compared (600), and a) if the calculated slopes, αc, are the same, the slope is used for determining (700) optical density of the solution, or b) if the calculated slopes, αc, are not the same, the steepest slope of the calculated slopes is used for determining (701a) optical density of the solution, or the slope of the calculated slopes being in the range of an absorbance reading of 0.01 to 2 is used for determining (701b) optical density of the solution.

The invention discloses a surface plasmon resonance imaging system for expanding a dynamic range through a multi-mode optical path, and relates to the technical field of microscopic imaging, a laser outputs a monochromatic and linearly polarized continuous wave beam, a first dynamic beam deflection device performs dynamic angle deflection adjustment on the continuous wave beam, a prism is used for providing a total reflection interface, and a second dynamic beam deflection device is used for performing dynamic angle deflection adjustment on the continuous wave beam. Exciting an evanescent wave; a surface plasma resonance effect is generated through interaction of the SPR detection chip and evanescent waves, and sample characteristics are detected; the flow cell provides a sample flow channel, so that a sample is in full contact with the surface of the SPR detection chip; the second dynamic light beam deflection device cooperatively adjusts the propagation direction of reflected light on the surface of the SPR detection chip, and the imaging detection device captures a high-spatial-resolution SPR image formed by the reflected light and transmits the image to the upper computer in real time for processing and analysis. According to the invention, the change of the resonance angle can be tracked in real time, and high-sensitivity and high-resolution monitoring of the dynamic process of biomolecule interaction is realized.

Cas-cleavable reporter systems, CRISPR-Cas systems thereof, and methods of use thereof in CRISPR-Cas based diagnostics. Cas-cleavable reporter systems may generate a luminescent, fluorescent, or other detectable signal upon Cas-collateral cleavage of one or more reporter system components. CRISPR-Cas systems may comprise a Cas protein having collateral cleavage activity, guide molecules, and the Cas-cleavable reporter system. Cas-cleavable reporter systems may be a split luciferase reporter system, at least one element of which is bound to beads. For multiplexing, CRISPR-Cas systems may comprise a Cas-cleavable quenched reporter system, optionally a Cas-cleavable quenched fluorescent reporter system, a Cas protein having collateral cleavage activity, and target-specific guide molecules attached to color-coded beads. CRISPR-Cas systems may further comprise amplification reagents. Methods may apply said CRISPR-Cas systems to flow cell, well-plate, or other devices, and may measure detectable signals by microscopic, plate reader, or other methods.

The utility model provides an electrode device for detecting Zeta potential of a high-concentration sample by electrophoresis light scattering, which comprises a laser, a high-concentration sample electrode and a photoelectric detector, an incident laser beam emitted by the laser is incident in a manner of being vertical to the outer vertical surface of a micro-flow cell, and an optical fiber head of the photoelectric detector is positioned in a direction forming an angle of 12 degrees with the laser beam; the high-concentration sample electrode adopts a flow-through design, and the minimum optical path of a micro flow cell is 1.5 mm; a sample can be added into the micro-flow cell through the silicone tube. According to the utility model, the Zeta potential test requirement of a high-concentration sample can be met, the minimum sample quantity is 57 microliters, the electrode cost is low, the electrode can be replaced, and the Zeta potential titration test can be carried out by being connected with an automatic titrator.

A flow path system, a gene sequencer and a reagent recovery method. The flow path system comprising at least two reagent storage containers, a flow cell, a shunting module and a fluid power unit. The flow cell is connected to the at least two reagent storage containers. The shunting module comprises a shunt structure and at least two shunt channels. The fluid power unit is connected to the shunting module, the fluid power unit is selectively connected to one of the at least two shunt channels, and the fluid power unit is configured to drive a forward flow of the reagent from the reagent storage container toward the shunting module, and the fluid power unit is further configured to drive a reverse flow of the reagent from the shunting module toward the reagent storage container.

A noninvasive system and method for simultaneous monitoring of species in a cell culture medium is described. Instead of direct contact with the culture media, the measurements can be made through permeable membranes via either a port in the culture vessel wall or a port in a flow cell. The noninvasive monitoring system and method can offer accurate, and contamination-minimized monitoring of critical process parameters including dissolved O2, pH, and dissolved CO2. These advancements will enhance the control and optimization of cell culture processes, promising improved cell culture performance.

The invention relates to the technical field of intelligent monitoring, and discloses a full-automatic sediment monitoring method and system.The method comprises the steps that a water sampling and distributing unit samples a target water area based on real-time sampling control parameters and conveys a sampled first water sample to a flow cell unit based on sampling control parameters; the flow cell unit simulates and forms a water sample monitoring water area environment matched with the target water area condition; the detection unit detects the water sample in the flow cell unit through a target detection method based on the detection control parameters to obtain a sediment detection result at least containing the sediment content; the reserved sample unit is used for extracting a reserved sample water sample for verifying the sediment detection result from the flow cell unit based on the reserved sample control parameters, and storing the reserved sample water sample and corresponding multi-dimensional water sample parameters. Therefore, the sediment condition of the related water area can be automatically and intelligently tested based on the full-automatic sediment monitoring system, and the testing efficiency and testing accuracy of the sediment condition can be improved.

The present disclosure relates to an optical system for a sample processing instrument including a flow cell with a detection channel for passage and detection of a sample. The optical system includes: a laser source; a collimating device configured to collimate beam emitted from the laser source; a focusing lens configured to focus the light beam from the laser source within the detection channel; and a reshaping device disposed between the collimating device and the focusing lens and configured to reshape spot of the collimated light beam. The reshaping device includes a first prism pair including two prisms being adjustable relative to each other so that the beam of the laser source has a predetermined size in a first direction. A sample processing instrument including the above optical system and flow cell is further provided therein.

A resealable fluidic device for conducting assays of a biological sample comprises a frame having a sample opening; and a cover slide configured to be received in the frame; wherein the frame is configured to receive a base slide on which a biological sample is configured to be disposed; wherein the cover slide and the base slide are configured to form a flow cell chamber housing the biological sample, and the flow cell chamber is configured to be aligned with the sample opening when the frame, the cover slide, and the based slide are assembled.

Provided herein are apparatuses and methods of releasing or converting a gas. In some embodiments, an apparatus may include a gas flow cell having an inlet and an outlet for flowing a gas through said gas flow cell; a structured material within said gas flow cell, wherein the structured material has an electrical conductivity selected from the range of 3×10−15 S / m to 6.3×107 S / m; and a plasma source integrated with said gas flow cell, the plasma source being configured to generate a plasma within a portion of the gas flow cell, the plasma source comprising: a first electrode and a second electrode within said gas flow cell; and a power source configured to provide voltage to the first and second electrodes.

The present invention discloses a Raman spectroscopy system designed to enhance the accuracy and reliability of fluid sample analysis. The system includes a flow cell that integrates an optical probe specifically optimized for Raman spectrometry measurements. To ensure precise measurements, the flow cell incorporates a fluid inlet and outlet for controlled routing of the sample. Significantly, an anti-reflective, non-fluorescent material is positioned opposite the optical interface of the flow cell. By introducing an anti-reflective, nonfluorescent measurement arrangement into the sample analysis process, it becomes possible to improve the overall quality of Raman spectra and enable more precise identification and characterization of samples.

The invention discloses a fluorescence detector based on an off-axis parabolic mirror, and belongs to the technical field of liquid chromatographic analysis, the fluorescence detector comprises a light source light path, an excitation monochromatic light path, a flow cell light path, a fluorescence collection light path, a fluorescence emission light path and a detector which are arranged in sequence, and the light source light path generates and converges ultraviolet-visible light; the converged ultraviolet-visible light is converted into monochromatic light with different wavelengths through the monochromatic light excitation path, the monochromatic light irradiates a to-be-detected sample in the flow cell light path to generate fluorescence, the fluorescence collection light path collects and converges the fluorescence, the converged fluorescence is divided into monochromatic fluorescence with different wavelengths through the fluorescence emission light path, and the monochromatic fluorescence is finally detected by the detector. The light source light path and the fluorescence collection light path both adopt off-axis parabolic reflectors to replace lenses, so that the influence of chromatic aberration is eliminated, and the utilization rate of optical energy is improved; the two off-axis parabolic reflectors are matched with each other, so that high spectral resolution is realized, and a simulation imaging light spot is less than 20 microns.

Disclosed herein are sequencing systems and sequencing methods for training neural networks and for utilizing the trained neural networks for sequencing analysis after acquiring flow cell images using the sequencing systems. The sequencing systems disclosed herein can include Field-Programmable Gate Array (FPGAs), artificial intelligence (AI) chips, or a combination thereof.

The invention discloses an activated sludge fluorescence online detection device based on a flow cell, and relates to the field of activated sludge biomass detection.The activated sludge fluorescence online detection device comprises a fluorescence spectrophotometer and a fluorescence spectrophotometer sample table arranged on the fluorescence spectrophotometer, and the fluorescence spectrophotometer is connected with a processor through a cable; the fluorescence spectrophotometer sample table is connected with a sample cup through a guide pipe, activated sludge is arranged in the sample cup, and a peristaltic pump is arranged on the guide pipe; the activated sludge continuously flows through the fluorescence spectrophotometer sample table through the guide pipe for three-dimensional fluorescence detection through the peristaltic pump, sludge sedimentation in a traditional cuvette is avoided, measurement is simple and convenient, and on-line real-time monitoring of the activated sludge biomass can be achieved.