161 results about "Biological particles" patented technology

The disclosure provides systems and methods for droplet processing. For example, a method can include providing a first droplet and a second droplet. In some cases, the first droplet may have a first concentration of a reagent and the second droplet may have a second concentration of the reagent. The second droplet may comprise a bead or a biological particle. The method can also include subjecting the first droplet and the second droplet to conditions sufficient to transfer the reagent from the first droplet to the second droplet, thereby decreasing the first concentration in the first droplet and increasing the second concentration in the second droplet.

The invention discloses a modified microfiltration membrane for enriching biological particles and a preparation method and application thereof, and belongs to the technical field of biological separation.The preparation method of the modified microfiltration membrane comprises the following steps that a mixed cellulose ester microfiltration membrane is provided and subjected to alkali liquor activating treatment; placing the activated membrane in a weakly alkaline buffer solution for balancing; the preparation method comprises the following steps: in a weakly alkaline buffer solution, firstly contacting the surface of the membrane with a tannic acid solution to carry out a first reaction, and then directly adding a polyethyleneimine solution to carry out a second reaction under the condition of not replacing a system, so as to form a phenol-amine cross-linked network on the surface of the membrane in situ; sodium cyanoborohydride is continuously added for reduction stabilization treatment, and the modified microporous filter membrane is obtained. The modified microporous filter membrane prepared by modifying the microporous filter membrane with tannic acid and polyethyleneimine can realize one-step synchronous enrichment of all negatively charged targets, and the problems of target loss, efficiency loss and the like caused by a multi-step process are avoided from the source.

The invention discloses a method for preparing a high-capacity sodium ion material based on precursor particle size screening and application, and belongs to the technical field of sodium ion batteries. According to the method, bamboo-based biomass is used as a raw material, and air pre-carbonization and high-temperature refining are performed to prepare the sodium ion material. Before pre-carbonization, crushing and screening are adopted to control the granularity of the bamboo-based biological particles, so that the morphology and the size of the precursor are more uniform, the pore structure of hard carbon is optimized, the energy storage performance of the precursor is improved, meanwhile, the sodium ion negative electrode material prepared by the preparation method is applied to a sodium ion battery, the capacity can reach 384.8 mAh / g, the preparation method is simple, the cost is low, and the method is suitable for industrial production. And the method is suitable for large-scale production.

The invention relates to the technical field of micro-fluidic biological detection, and discloses a micro-fluidic chip for rapidly detecting bacteria based on multi-mode detection, a preparation method and a detection method.The chip comprises a PDMS runner layer and a glass substrate layer which are sequentially arranged from top to bottom; the PDMS flow channel layer comprises an injection area used for receiving a to-be-detected sample and carrying out pretreatment; the focusing area is used for realizing three-dimensional fluid focusing of the detected particles through a coupling effect of viscoelasticity and an inertial effect; the detection area is used for performing electrical impedance detection and fluorescence detection on the focused particles; the outflow area is used for collecting and outputting the detected sample; the PDMS flow channel layer and the glass substrate layer are bonded through oxygen plasma to form a sealed channel structure, and the sealed channel structure is used for realizing electrical impedance and fluorescence dual-mode synchronous detection of microbial particles in a single flow channel. By optimizing the fluid focusing structure and the electrode design, high-precision synchronous detection of the microbial particles is realized.

Highly efficient and rapid filtration-based concentration devices, systems and methods are disclosed with sample fluidic lines and a filter packaged in a disposable tip which concentrates biological particles that are suspended in liquid from a dilute feed suspension. A sample concentrate or retentate suspension is retained while eliminating the separated fluid in a separate flow stream. The concentrate is then dispensed from the disposable tip in a set volume of elution fluid. Suspended biological particles include such materials as proteins / toxins, viruses, DNA, and / or bacteria in the size range of approximately 0.001 micron to 20 microns diameter. Concentration of these particles is advantageous for detection of target particles in a dilute suspension, because concentrating them into a small volume makes them easier to detect and identify. A single-use pipette tip includes fluid ports for aspirating the sample and connecting to a concentrating unit.

The invention provides a cytometer and a cell rotation method, the cytometer comprises a piezoelectric substrate made of a piezoelectric material and a transducer, and the piezoelectric substrate comprises a sample placing area for placing cell sap to be detected; the transducer comprises a first interdigital transducer and a second interdigital transducer which are oppositely arranged on the piezoelectric substrate, and the sample placement area is located between the first interdigital transducer and the second interdigital transducer; wherein the first interdigital transducer and the second interdigital transducer are used for driving the piezoelectric substrate to vibrate so as to spread surface acoustic waves in cell sap in the sample placement area, the width of an area corresponding to the cell sap in the sample placement area is related to the wavelength of the surface acoustic waves, and the surface acoustic waves are used for forming at least two vortexes in the cell sap; the vortex drives cells in the cell sap to rotate orderly, the area width is the width of the cell sap in the propagation direction of the surface acoustic wave, the cells in multiple vortexes can be clearly observed in the cell rotation process, and in-situ control over multiple biological particles is achieved.

A biological particle counting system can include: an impedance particle counter comprising at least one sample aperture; a pump configured to pull particles through the at least one sample aperture of the impedance particle counter for counting, the pump producing a vacuum pressure; and a stepper motor configured to adjust a speed of the pump to substantially maintain the vacuum pressure.

A correction and capture method for a biological particle capture device includes: providing an image observation unit facing a side of a carrier platform on an observation platform and orientating a view region of the image observation unit towards a carrier region of the carrier platform for taking images; performing a fixed point displacement on the observation platform by the image observation unit based on several observation basic coordinates on an observation platform coordinate system and defining a correction point in an image of the view region when the image observation unit is positioned at one observation basic coordinate and take the image; controlling a capture portion of the particle capture tool to enter the image of the view region; adjusting the capture portion to align with the correction point and recording a capture correction coordinate by the terminal device.

The utility model provides non-contact biological particle treatment equipment and a biological particle treatment device. The biological particle processing device comprises a liquid droplet generating chamber, a working chamber communicated with the liquid droplet generating chamber, and a sorting chamber communicated with the working chamber. The droplet generating chamber is configured to receive a first liquid, a biological particle located within the first liquid, and a second liquid insoluble in the first liquid. The droplet generating chamber is used for enabling the flow of the second liquid to be staggered with the flow of the first liquid, so that the biological particles and the first liquid part around the biological particles jointly generate a biological particle droplet flowing to the working chamber after penetrating through the second liquid; the biological particles are cultured or tested through the first liquid. Therefore, the biological particle liquid drop can protect at least one biological particle located in the biological particle liquid drop, and culture work or detection can be completed while the biological particle liquid drop moves.

The present invention pertains to specific bioparticules at the surface of which are expressed multimeric protein. The bioparticles according to the present invention comprise an envelope consisting of a plasma membrane; and at least one type I or II transmembrane fusion protein anchored in said membrane, said fusion protein comprising successively a) a first monomer of a multimeric protein of interest, b) a coiled-coil domain or oligomerization sequence; and c) a domain for anchoring in the plasma membrane, consisting of a transmembrane segment and a cytosolic segment. Fragments a) and b) are exposed at the surface of the bioparticle, and fragment a) is bound to a second monomer of said multimeric protein by means of a bond which is not a peptide bond. The bioparticles according to the present invention can be used in therapy such as immunotherapy. The present invention also pertains to methods for producing such bioparticles.

Methods for distinguishing particles in a fluid sample are disclosed. In one embodiment, the method includes acquiring a background SIMI image and a background brightfield image of a membrane filter while the membrane filter is free of a fluid sample, introducing a fluid sample onto the membrane filter, acquiring a SIMI image and a brightfield image of filtered particles resting on the membrane filter, distinguishing between the filtered particulates and the membrane filter based on the background SIMI image, generating a particle mask based on the SIMI image, and detecting beads via the particle mask. Methods for distinguishing particulates include distinguishing between viable and non-viable cell populations, distinguishing between cellular and non-cellular particulates, distinguishing between biological and non-biological particulates, distinguishing between first and second protein types, determining stability of monoclonal antibody drugs, identifying beads in a cell therapy, and detecting bacteria.

1. The name of the design product: the main body of a biological particle separator with a transparent membrane layer. 2. The use of the design product: the design product is the main body of a biological particle separator with a transparent membrane layer, which is a device or system for separating specific biological particles (such as cells, organelles, proteins, nucleic acids, viruses, etc.) from complex mixtures. 3. The design points of the design product: the shape shown by solid lines in the view. 4. The picture or photo that best indicates the design points: Design 1 perspective view 1. 5. Design 1 is designated as the basic design. 6. Other circumstances that need to be explained: the part represented by solid lines in the view is the design for which protection is sought, and the part represented by dashed lines does not constitute the design content for which protection is sought. The circular membrane marked by letters A-F in the view is transparent.

The invention relates to the technical field of soil conditioners, in particular to an efficient preparation method of a micro-nano biochar conditioner, which comprises the following steps: crushing and compressing raw materials, and heating under a nitrogen filling condition to obtain a biochar matrix; the preparation method comprises the following steps: ball-milling a biochar matrix, mixing the ball-milled biochar matrix with deionized water, sequentially carrying out stirring, ultrasonic oscillation and filter membrane filtration at room temperature, and then carrying out freezing and freeze-drying; when it is judged that the preparation of the micro-nano biological particles does not meet the preset standard according to the particle evaluation value, secondary judgment is conducted, or the reason for the preparation of the micro-nano biological particles does not meet the preset standard is determined; mixing the micro-nano biological particles meeting the preset standard with the bacterial suspension, and oscillating by using a shaking table according to a preset shaking table rotating speed, so as to obtain the micro-nano biological carbon conditioner; when it is judged that the preparation of the micro-nano biochar conditioner does not meet the preset standard according to the adsorption characterization value, the rotating speed of a shaking table is increased, or the centrifugal rotating speed is increased, and the product quality is improved.

The present application relates to the technical field of tumor cell detection chip, in particular to a kind of based on inertial microfluidic tumor cell mechanical property detection device and method, the present application adopts sine microfluid channel inertial focusing cell, and the mechanical property detection of cell multiple deformation in channel is carried out by high-speed camera.The cell suspended in PBS is subjected to the combined action of inertial force and dean force in sine microfluid channel, and the single-column focusing of cell can be realized in a wide flow rate range, and the recognition accuracy of optical detection is improved.Cell is subjected to shear force, tensile force and wall collision in microfluid channel and generates three deformations, and different types and sizes of force make cell produce different degrees and shapes of deformation.The size, roundness, deformation and circumscribed rectangle size of cell in different deformations are extracted, and the classification model of species is trained using machine learning method, to identify cell species.The present application is suitable for the detection of various cells in blood samples and biological particles in other biological samples.

Chromatographic media comprising porous beads having an inner porous core and an outer porous shell are used for the chromatographic separation of enveloped or membrane-bearing biological particles from impurities such as contaminating DNA and / or proteins. The core is capable of binding molecules through hydrophobic interactions; however, the pore size of the shell prevents particles with a size of 20 nm or larger from penetrating into the beads and interacting with the core. Separation is performed at a pH below 7.4. The enveloped or membrane-bearing biological particles may have been subjected to a prior chromatographic capture step. When used for the purification of enveloped viral particles, the method of the present invention has been found to result in a significantly higher rate of infectious viral particles.

The present application belongs to the technical field of microfluidic chip, and particularly relates to a two-section cell sample sorting microfluidic chip and a sorting method thereof. A rectangular array composed of multiple columns of micro-pillars is arranged in the main flow channel of the chip, and the column offset angle between adjacent two columns of micro-pillars periodically changes along the sample flow direction. In one change cycle, each column of micro-pillars is first deflected by a preset gradient angle column by column along a first direction until the cumulative deflection angle reaches a limit angle, and then is deflected along a second opposite direction until the limit angle is reached, and the cycle is repeated. By arranging the micro-pillar array with periodically changing column offset angle, a dynamic flow field is created in the main flow channel, and high stiffness fibrous particles are actively induced to rotate and change their poses, and their effective hydrodynamic size is dynamically amplified, so that accurate sorting of fibrous biological particles based on morphology and length is realized, and the risk of sample damage and clogging is reduced, which is suitable for the rapid detection needs of clinical complex samples.

This invention discloses a method and system for detecting the strongest Rayleigh acoustic current in immobilized microdroplets. Specifically, it involves: calculating the acoustic current suppression coefficient through experimental quantitative testing to establish a quantitative mapping relationship between acoustic current intensity and droplet contact angle; utilizing an electrowetting structure composed of interdigitated electrodes, a dielectric layer, and a hydrophobic layer to actively regulate the droplet contact angle through an applied voltage; and dynamically adjusting the applied voltage in real time by monitoring the droplet vibration amplitude, particle velocity, and contact angle through a collaborative control program to maintain the acoustic current intensity at its strongest. This invention also provides a detection system for implementing the above method, including an indium tin oxide transparent interdigitated electrode substrate, a lead zirconate titanate piezoelectric transducer, a laser Doppler vibrometer, a microscope, and a collaborative control module. This invention can maximize and dynamically maintain the Rayleigh acoustic current intensity in immobilized microdroplets, significantly accelerating the solid-liquid interface mass transfer process, and is applicable to biochemical analysis fields such as rapid immunoassay and biological particle mixing.

The present invention relates to a method and associated system for characterizing biological particles in aerosol form (i.e., suspended in an ambient gas) using laser-induced plasma spectroscopy, comprising the steps of: a) collecting (Ea) the ambient gas containing the biological particles intended to be characterized; b) generating (Eb) a jet of said particles (JAB) in a vacuum chamber (CH); c) transmitting a laser beam (FL) in the form of pulses and focusing the laser beam in the vacuum chamber (CH) transversely to the propagation direction of the jet of particles (JP) so as to capture in a focusing volume (VF) the laser beam (FL) and at least one individual particle (N) of the jet. P ), wherein the plasma sends other particles that are specific to the interaction between the laser beam and individual particles of the jet; d) collecting the particles sent by the plasma (Ed); and e) performing spectroscopic analysis of said particles (Ee) to ultimately characterize the biological particles.

1. Name of the product in this design: Flow Cytometer. 2. Purpose of this design: For rapid quantitative analysis and sorting of cells or other biological particles in liquids using multiple parameters. 3. The key design feature of this product is its shape. 4. The image or photograph that best illustrates the design's key points: 3D view 1.

The present application relates to fine particle sensing technology, and particularly relates to a microcavity vibration spectrum system and method for measuring inherent vibration spectrum of fine particles. The microcavity vibration spectrum system comprises an inherent vibration excitation light path unit and a microcavity detection light path unit; the inherent vibration excitation light path unit is sequentially provided with a pulsed light source, a vibrating mirror, an objective lens and a particle to be measured; and the microcavity detection light path unit is sequentially provided with a tunable laser light source, an adjustable attenuator, a polarization controller, a coupling optical waveguide, an optical microcavity, a photodetector and an oscilloscope. The present application can realize precise measurement of sound waves caused by particle vibration of mesoscale particles and biological particles with inherent vibration frequency in the sub-megahertz-gigahertz frequency range.

The invention discloses a biological particle chemical cracking method based on low-voltage direct current and application, the cracking method adopts an electrode to realize efficient cracking of biological particles under a working voltage of less than or equal to 4.5 V. The cracking method has the core technical advantages that: 1) low-voltage cracking can effectively avoid a Joule heating effect and ensure the integrity of nucleic acid; electrochemical damage to nucleic acid under high voltage is avoided; (2) contents released by the biological particles can be directly used for nucleic acid amplification without centrifugal purification, so that complicated procedures such as centrifugation and purification required by traditional nucleic acid extraction are omitted; and 3) compared with a commercial lysis solution, the nucleic acid extraction efficiency of electric lysis is higher than that of the commercial lysis solution by one order of magnitude. The technology is matched with a miniaturized detection system, the whole process can be completed within 25 minutes, the detection sensitivity reaches 101 CFU / mL, and compared with a traditional method, the method has the advantages that the operation time is remarkably shortened, the cost is effectively reduced, and the method is particularly suitable for microfluidic automatic nucleic acid detection.

The present disclosure provides materials and methods for detecting the presence of a target, such as a nucleic acid, biomolecule or biological particle, or virus genome in a sample. The present disclosure further provides point of care detection methods that cane done quickly and simply, optionally without sophisticated instrumentation. The present disclosure further provides improved methods for detecting and quantifying detection signals from various emulsions.

The application provides a microfluidic chip and a biological particle separation device based on the microfluidic chip, which comprises a main flow channel, a plurality of bypass flow channels and an electrode flow channel. The electrode flow channel is arranged on both sides of the side wall of the main flow channel and forms a plurality of electrode zones along the extension direction of the main flow channel, so as to provide a non-uniform electric field for the main flow channel; the plurality of bypass flow channels are sequentially communicated with the main flow channel along the extension direction of the main flow channel, so that the electrode zones and the bypass flow channels are alternately and spacedly arranged along the extension direction of the main flow channel. Thus, after the sample liquid is injected into the main flow channel, different target biological particles in the sample liquid can produce different lateral deviations under the dielectrophoresis force of one or more electrode zones, so that the target biological particles can flow out from the liquid outlet of the corresponding bypass flow channel or the main flow channel, and the efficient separation of biological particles of multiple sizes and types is realized.

According to the multifunctional emergency phosphorus removal aeration device for the lake, the phosphorus removal system is adopted, sewage to be treated is guided into the water inlet pipe after being preliminarily filtered, more than 90% of phosphorus can be removed from the sewage passing through the biological filter layer under the action of the phosphorus adsorption plate, and the water after phosphorus removal is guided into the system, so that the sewage can be recycled. Under the action of oxygen bubbles generated by the aeration pipe grid, microorganisms released by the bottom microorganism particle slow release tank are diffused into a large water area, so that the treatment of eutrophicated rivers and lakes is improved. The aeration system is simple in structure and convenient to use, the aeration system is not provided with a mechanical rotating part in water, the water body is not vibrated violently, organisms in the water body are not damaged, the water body is not easily stirred, deposited pollutants are not easily released into the water body again, and on the other hand, a biological filtering layer formed by microorganisms attached to the ceramsite layer is low in price, resistant to corrosion, light in weight and convenient to use. The porous surface area is large, the stress resistance is good, and the generalizability is high.

A sensor device for detecting biological particles and non-biological particles includes: at least one light-source unit for radiating ultraviolet rays; and a light-condensing mirror unit including a first light-condensing mirror unit disposed in front of the at least one light source unit and a second condensing mirror unit spaced apart from the first light-condensing mirror unit.

The present application relates to a method for extracting lipids from microorganisms, comprising the steps of: (a) preparing a microbial fermentation solution containing lipids; (b) fluidized-bed drying the microbial fermentation solution of step (a) so as to form microbial granules; and (c) press-extracting the microbial granules of step (b), wherein a solvent is not used during lipid extraction.

Disclosed herein are fluidic devices configured for bioparticle processing, systems comprising said fluidic devices, and methods of bioparticle processing using said systems.

Provided is a novel cell capture method.　The present technology provides a bioparticle capture method, etc., in which at least a capture step, for capturing a bioparticle having a lipid membrane using a lipid-molecule-modified nucleic acid having a branched structure and a plurality of modified lipid molecules, is performed, and the distance from a branch point of the branched structure to a hydroxyl group that binds a lipid molecule is 3 Å or more.

Compositions and methods for hydrogel formation, such as microcapsule hydrogel shell formation, are disclosed herein. Methods and compositions herein yield microcapsule populations having predictable microcapsule properties such as shell widths and microcapsule diameters, and may comprise bioparticles that may be analyzed using reactions performed in series. Approaches using biocompatible catalysis of hydrogel formation, as well as uncatalyzed hydrogel crosslinking approaches, are disclosed herein.

The present disclosure relates to a chromatography system comprising a buffer valve arrangement configured to allow independent control of a first buffer feed and a second buffer feed; a pump arrangement configured to supply the first buffer feed, the second buffer feed, and a feed comprising a biological target compound and one or more impurities; a selector valve arrangement comprising a first chromatography device selector valve; a first chromatography device comprising a first chromatography material comprising a support material functionalized with a ligand wherein the ligand comprises an anion exchange group or an affinity group having a binding affinity for a biological target compound; a second chromatography device comprising a second chromatography material, the second chromatography material comprising a regulatory chromatography material; wherein the selector valve arrangement is configured to be able to separate a biological target compound from one or more impurities by allowing a feed comprising the biological target compound and the one or more impurities to continuously pass through the first chromatography device and the second chromatography device, and wherein the first chromatography device and the second chromatography device are configured to be connected in series. The present disclosure also provides uses of the chromatography system and a method for separating enveloped or membranous bio-particles from one or more impurities.