101 results about "Biological particles" patented technology

The invention discloses a modified microfiltration membrane for enriching biological particles and a preparation method and application thereof, and belongs to the technical field of biological separation.The preparation method of the modified microfiltration membrane comprises the following steps that a mixed cellulose ester microfiltration membrane is provided and subjected to alkali liquor activating treatment; placing the activated membrane in a weakly alkaline buffer solution for balancing; the preparation method comprises the following steps: in a weakly alkaline buffer solution, firstly contacting the surface of the membrane with a tannic acid solution to carry out a first reaction, and then directly adding a polyethyleneimine solution to carry out a second reaction under the condition of not replacing a system, so as to form a phenol-amine cross-linked network on the surface of the membrane in situ; sodium cyanoborohydride is continuously added for reduction stabilization treatment, and the modified microporous filter membrane is obtained. The modified microporous filter membrane prepared by modifying the microporous filter membrane with tannic acid and polyethyleneimine can realize one-step synchronous enrichment of all negatively charged targets, and the problems of target loss, efficiency loss and the like caused by a multi-step process are avoided from the source.

The invention relates to the technical field of micro-fluidic biological detection, and discloses a micro-fluidic chip for rapidly detecting bacteria based on multi-mode detection, a preparation method and a detection method.The chip comprises a PDMS runner layer and a glass substrate layer which are sequentially arranged from top to bottom; the PDMS flow channel layer comprises an injection area used for receiving a to-be-detected sample and carrying out pretreatment; the focusing area is used for realizing three-dimensional fluid focusing of the detected particles through a coupling effect of viscoelasticity and an inertial effect; the detection area is used for performing electrical impedance detection and fluorescence detection on the focused particles; the outflow area is used for collecting and outputting the detected sample; the PDMS flow channel layer and the glass substrate layer are bonded through oxygen plasma to form a sealed channel structure, and the sealed channel structure is used for realizing electrical impedance and fluorescence dual-mode synchronous detection of microbial particles in a single flow channel. By optimizing the fluid focusing structure and the electrode design, high-precision synchronous detection of the microbial particles is realized.

A correction and capture method for a biological particle capture device includes: providing an image observation unit facing a side of a carrier platform on an observation platform and orientating a view region of the image observation unit towards a carrier region of the carrier platform for taking images; performing a fixed point displacement on the observation platform by the image observation unit based on several observation basic coordinates on an observation platform coordinate system and defining a correction point in an image of the view region when the image observation unit is positioned at one observation basic coordinate and take the image; controlling a capture portion of the particle capture tool to enter the image of the view region; adjusting the capture portion to align with the correction point and recording a capture correction coordinate by the terminal device.

1. The name of the design product: the main body of a biological particle separator with a transparent membrane layer. 2. The use of the design product: the design product is the main body of a biological particle separator with a transparent membrane layer, which is a device or system for separating specific biological particles (such as cells, organelles, proteins, nucleic acids, viruses, etc.) from complex mixtures. 3. The design points of the design product: the shape shown by solid lines in the view. 4. The picture or photo that best indicates the design points: Design 1 perspective view 1. 5. Design 1 is designated as the basic design. 6. Other circumstances that need to be explained: the part represented by solid lines in the view is the design for which protection is sought, and the part represented by dashed lines does not constitute the design content for which protection is sought. The circular membrane marked by letters A-F in the view is transparent.

The present application relates to the technical field of tumor cell detection chip, in particular to a kind of based on inertial microfluidic tumor cell mechanical property detection device and method, the present application adopts sine microfluid channel inertial focusing cell, and the mechanical property detection of cell multiple deformation in channel is carried out by high-speed camera.The cell suspended in PBS is subjected to the combined action of inertial force and dean force in sine microfluid channel, and the single-column focusing of cell can be realized in a wide flow rate range, and the recognition accuracy of optical detection is improved.Cell is subjected to shear force, tensile force and wall collision in microfluid channel and generates three deformations, and different types and sizes of force make cell produce different degrees and shapes of deformation.The size, roundness, deformation and circumscribed rectangle size of cell in different deformations are extracted, and the classification model of species is trained using machine learning method, to identify cell species.The present application is suitable for the detection of various cells in blood samples and biological particles in other biological samples.

Chromatographic media comprising porous beads having an inner porous core and an outer porous shell are used for the chromatographic separation of enveloped or membrane-bearing biological particles from impurities such as contaminating DNA and / or proteins. The core is capable of binding molecules through hydrophobic interactions; however, the pore size of the shell prevents particles with a size of 20 nm or larger from penetrating into the beads and interacting with the core. Separation is performed at a pH below 7.4. The enveloped or membrane-bearing biological particles may have been subjected to a prior chromatographic capture step. When used for the purification of enveloped viral particles, the method of the present invention has been found to result in a significantly higher rate of infectious viral particles.

The present application belongs to the technical field of microfluidic chip, and particularly relates to a two-section cell sample sorting microfluidic chip and a sorting method thereof. A rectangular array composed of multiple columns of micro-pillars is arranged in the main flow channel of the chip, and the column offset angle between adjacent two columns of micro-pillars periodically changes along the sample flow direction. In one change cycle, each column of micro-pillars is first deflected by a preset gradient angle column by column along a first direction until the cumulative deflection angle reaches a limit angle, and then is deflected along a second opposite direction until the limit angle is reached, and the cycle is repeated. By arranging the micro-pillar array with periodically changing column offset angle, a dynamic flow field is created in the main flow channel, and high stiffness fibrous particles are actively induced to rotate and change their poses, and their effective hydrodynamic size is dynamically amplified, so that accurate sorting of fibrous biological particles based on morphology and length is realized, and the risk of sample damage and clogging is reduced, which is suitable for the rapid detection needs of clinical complex samples.

This invention discloses a method and system for detecting the strongest Rayleigh acoustic current in immobilized microdroplets. Specifically, it involves: calculating the acoustic current suppression coefficient through experimental quantitative testing to establish a quantitative mapping relationship between acoustic current intensity and droplet contact angle; utilizing an electrowetting structure composed of interdigitated electrodes, a dielectric layer, and a hydrophobic layer to actively regulate the droplet contact angle through an applied voltage; and dynamically adjusting the applied voltage in real time by monitoring the droplet vibration amplitude, particle velocity, and contact angle through a collaborative control program to maintain the acoustic current intensity at its strongest. This invention also provides a detection system for implementing the above method, including an indium tin oxide transparent interdigitated electrode substrate, a lead zirconate titanate piezoelectric transducer, a laser Doppler vibrometer, a microscope, and a collaborative control module. This invention can maximize and dynamically maintain the Rayleigh acoustic current intensity in immobilized microdroplets, significantly accelerating the solid-liquid interface mass transfer process, and is applicable to biochemical analysis fields such as rapid immunoassay and biological particle mixing.

The present invention relates to a method and associated system for characterizing biological particles in aerosol form (i.e., suspended in an ambient gas) using laser-induced plasma spectroscopy, comprising the steps of: a) collecting (Ea) the ambient gas containing the biological particles intended to be characterized; b) generating (Eb) a jet of said particles (JAB) in a vacuum chamber (CH); c) transmitting a laser beam (FL) in the form of pulses and focusing the laser beam in the vacuum chamber (CH) transversely to the propagation direction of the jet of particles (JP) so as to capture in a focusing volume (VF) the laser beam (FL) and at least one individual particle (N) of the jet. P ), wherein the plasma sends other particles that are specific to the interaction between the laser beam and individual particles of the jet; d) collecting the particles sent by the plasma (Ed); and e) performing spectroscopic analysis of said particles (Ee) to ultimately characterize the biological particles.

1. Name of the product in this design: Flow Cytometer. 2. Purpose of this design: For rapid quantitative analysis and sorting of cells or other biological particles in liquids using multiple parameters. 3. The key design feature of this product is its shape. 4. The image or photograph that best illustrates the design's key points: 3D view 1.

The present application relates to fine particle sensing technology, and particularly relates to a microcavity vibration spectrum system and method for measuring inherent vibration spectrum of fine particles. The microcavity vibration spectrum system comprises an inherent vibration excitation light path unit and a microcavity detection light path unit; the inherent vibration excitation light path unit is sequentially provided with a pulsed light source, a vibrating mirror, an objective lens and a particle to be measured; and the microcavity detection light path unit is sequentially provided with a tunable laser light source, an adjustable attenuator, a polarization controller, a coupling optical waveguide, an optical microcavity, a photodetector and an oscilloscope. The present application can realize precise measurement of sound waves caused by particle vibration of mesoscale particles and biological particles with inherent vibration frequency in the sub-megahertz-gigahertz frequency range.

The present disclosure provides materials and methods for detecting the presence of a target, such as a nucleic acid, biomolecule or biological particle, or virus genome in a sample. The present disclosure further provides point of care detection methods that cane done quickly and simply, optionally without sophisticated instrumentation. The present disclosure further provides improved methods for detecting and quantifying detection signals from various emulsions.

The application provides a microfluidic chip and a biological particle separation device based on the microfluidic chip, which comprises a main flow channel, a plurality of bypass flow channels and an electrode flow channel. The electrode flow channel is arranged on both sides of the side wall of the main flow channel and forms a plurality of electrode zones along the extension direction of the main flow channel, so as to provide a non-uniform electric field for the main flow channel; the plurality of bypass flow channels are sequentially communicated with the main flow channel along the extension direction of the main flow channel, so that the electrode zones and the bypass flow channels are alternately and spacedly arranged along the extension direction of the main flow channel. Thus, after the sample liquid is injected into the main flow channel, different target biological particles in the sample liquid can produce different lateral deviations under the dielectrophoresis force of one or more electrode zones, so that the target biological particles can flow out from the liquid outlet of the corresponding bypass flow channel or the main flow channel, and the efficient separation of biological particles of multiple sizes and types is realized.

The present application relates to a method for extracting lipids from microorganisms, comprising the steps of: (a) preparing a microbial fermentation solution containing lipids; (b) fluidized-bed drying the microbial fermentation solution of step (a) so as to form microbial granules; and (c) press-extracting the microbial granules of step (b), wherein a solvent is not used during lipid extraction.

Disclosed herein are fluidic devices configured for bioparticle processing, systems comprising said fluidic devices, and methods of bioparticle processing using said systems.

Compositions and methods for hydrogel formation, such as microcapsule hydrogel shell formation, are disclosed herein. Methods and compositions herein yield microcapsule populations having predictable microcapsule properties such as shell widths and microcapsule diameters, and may comprise bioparticles that may be analyzed using reactions performed in series. Approaches using biocompatible catalysis of hydrogel formation, as well as uncatalyzed hydrogel crosslinking approaches, are disclosed herein.

The application discloses a liquid metal injection type annular micro-particle fractionation micro-fluidic device and method, relates to the technical field of micro-fluidics and biological particle separation, and comprises a micro-fluidic chip main body which is internally provided with a sample channel, positive and negative electrode channels which are arranged in the micro-fluidic chip main body and are parallelly distributed on the two sides of the sample channel respectively, a liquid metal electrode array which is composed of liquid metal filled and solidified in the electrode channels, and the liquid metal electrode array for generating a non-uniform electric field in the sample channel, wherein the electrode channels are in a meandering and returning structure, so that the liquid metal electrode array forms thick-wall side electrodes in the micro-fluidic chip main body; and the device realizes high-throughput, high-purity and low-damage label-free biological particle separation.

This invention discloses a high-precision positioning platform, which mainly includes a drive motor, a roller, a rope, a main slide, and a main guide rail. The output end of the drive motor is connected to the roller via a coupling. A rope is wound around the roller, with one end of the rope wound around the roller and the other end connected to the main slide. The main slide is slidably connected to the main guide rail, which is fixedly mounted on the frame. This positioning platform can drive the roller to rotate via the drive motor, causing the rope to wind around the roller, thereby driving the operating device on the main slide to achieve submicron and nanometer precision single-axis movement. It can be used for the precise manipulation of micro- and nano-sized biological particles such as single cells and single-cell extracellular vesicles.

The invention discloses a high-flux biological particle separation system based on a micro-fluidic chip. The high-flux biological particle separation system comprises a micro-fluidic BAW chip, a micro-fluidic cascade chip and a plurality of DLD separation units, wherein a separation outlet area in the microfluidic BAW chip is provided with an extracellular vesicle collection channel, and the radiation assembly comprises a first piezoelectric transducer and a second piezoelectric transducer which are respectively used for providing high-frequency bulk acoustic waves and low-frequency bulk acoustic waves; a liquid inlet channel of the microfluidic cascade chip is connected with an extracellular vesicle collection channel, and a fluid distribution area is provided with a plurality of parallel branch channels; and the DLD separation units are connected with the parallel branch channels in a one-to-one correspondence manner. Through reasonable configuration of the multi-stage separation structure, multi-target separation of biological particles with different sizes in a blood sample can be realized.

This invention discloses a method for early warning and detection of biological particulate matter in air, belonging to the field of environmental monitoring technology. It uses a high-speed, lensless diffraction imaging system to capture particulate matter diffraction ring images in real time, extracts gray-level co-occurrence matrix texture features and wavelet multi-scale features, fuses them to generate a real-time fusion vector, and constructs an index calculation model to calculate a biological index sequence. It identifies biological attributes and uses multi-class SVM for classification, statistically represents concentration by the number of events, and combines Poisson mutation detection to analyze the trend of change. When the concentration exceeds the standard or the trend is abnormal, it triggers a graded early warning. Through concentration adjustment feedback and multi-source data verification, it identifies the type of misjudgment and dynamically optimizes the equipment, model, and threshold. This method achieves high-precision identification, stable monitoring, and intelligent early warning of biological particulate matter in the air, improving detection sensitivity and robustness, and reducing the misjudgment rate.

A semifinished product for producing shaped bodies, comprising foamed biogranules obtained from biogranules, wherein the embryos have been removed from the biogranules. In a method for producing a semifinished product, the embryo is removed from a grain, more specifically corn, in order to obtain embryo-free biogranules, which are then converted to foamed biogranules.

Highly efficient and rapid filtration-based concentration devices, systems and methods are disclosed with sample fluidic lines and a filter packaged in a disposable tip which concentrate biological particles that are suspended in liquid from a dilute feed suspension. A sample concentrate or retentate suspension is retained while eliminating the separated fluid in a separate flow stream. The concentrate is then dispensed from the disposable tip in a set volume of elution fluid. Suspended biological particles include such materials as proteins / toxins, viruses, DNA, and / or bacteria in the size range of approximately 0.001 micron to 20 microns diameter. Concentration of these particles is advantageous for detection of target particles in a dilute suspension, because concentrating them into a small volume makes them easier to detect. A single-use pipette tip includes fluid ports for aspirating the sample and connecting to a concentrating unit.

The main purpose of this disclosure is to provide a bioparticle sorting device that is easy for each of two different types of users to use or operate.　This disclosure provides a bioparticle sorting device that has a first display mode for receiving input of operation control data relating to bioparticle sorting processing and a second display mode for displaying an operation screen for carrying out processing and operates in either the first display mode or the second display mode on the basis of identification information. In the first display mode, a processing condition setting screen relating to bioparticle sorting processing is displayed, and the bioparticle sorting device may receive operation control data input via the processing condition setting screen.

A chromatography medium comprising porous beads having an inner porous core and an outer porous shell is used for chromatographic separation of enveloped or membranous biological particles from impurities such as contaminant DNA and / or protein. The core is capable of binding molecules via hydrophobic interactions; however, the pore size of the shell does not allow particles having a size of 20 nm and larger to permeate into the bead and interact with the core. The separation is performed at a pH of less than 7.4. The enveloped or membranous biological particles may have been subjected to a prior chromatographic capture step. When used for purification of enveloped virus particles the inventive process was found to yield a remarkably high rate of infectious virus particles.

The disclosure provides systems and methods for droplet processing. For example, a method can include providing a first droplet and a second droplet. In some cases, the first droplet may have a first concentration of a reagent and the second droplet may have a second concentration of the reagent. The second droplet may comprise a bead or a biological particle. The method can also include subjecting the first droplet and the second droplet to conditions sufficient to transfer the reagent from the first droplet to the second droplet, thereby decreasing the first concentration in the first droplet and increasing the second concentration in the second droplet.

The present application relates to a kind of biological particle sorting flow channel and microfluidic chip, biological particle sorting flow channel includes first flow channel unit, the first flow channel unit includes oppositely arranged first side wall and second side wall, the first side wall and second side wall are asymmetric curved surface, the first flow channel unit near one side of first side wall or second side wall is excavated with deepening flow channel, the deepening flow channel is along the first flow channel unit extension direction setting.Set with asymmetric curved surface first flow channel unit, form focusing flow, deepening flow channel is along the first flow channel unit extension direction setting, break the liquid flow state near the first flow channel unit near deepening flow channel, so that inertial lift force and dean drag change, destroy original balance, so that the particle such as leukocyte near deepening flow channel side can produce disorderly movement state, avoid leukocyte aggregate band and circulating tumor cell overlap, benefit the recovery of circulating tumor cell.

The wet-wall cyclone cup biological particle collection efficiency evaluation method disclosed by the embodiment of the invention comprises the following steps: S1, arranging a biological aerosol closed experiment module, and arranging a wet-wall cyclone cup sampler and a six-stage Anderson sampler in the experiment module; s2, generating biological aerosol with set concentration in the experiment module; s3, simultaneously starting the wet wall cyclone cup sampler and the six-stage Anderson sampler for sampling; and S4, determining sampling results of the wet-wall cyclone cup sampler and the six-stage Anderson sampler, and determining the biological particle collection efficiency of the wet-wall cyclone cup according to the sampling results. Biological particles are directly adopted for testing, so that the collection efficiency of the wet-wall cyclone cup on the biological aerosol can be truly reflected, and evaluation deviation caused by characteristic difference between physical particles and actual biological particles is avoided; the method is simple, easy to repeat, simple in equipment, high in practicability and good in universality.

Highly efficient and rapid filtration-based concentration devices, systems and methods are disclosed with sample fluidic lines and a filter packaged in a disposable tip which concentrates biological particles that are suspended in liquid from a dilute feed suspension. A sample concentrate or retentate suspension is retained while eliminating the separated fluid in a separate flow stream. The concentrate is then dispensed from the disposable tip in a set volume of elution fluid. Suspended biological particles include such materials as proteins / toxins, viruses, DNA, and / or bacteria in the size range of approximately 0.001 micron to 20 microns diameter. Concentration of these particles is advantageous for detection of target particles in a dilute suspension, because concentrating them into a small volume makes them easier to detect and identify. A single-use pipette tip includes fluid ports for aspirating the sample and connecting to a concentrating unit.

A method for screening and detecting biological particles includes the following steps: providing a biological particle screening and detection system, comprising a flow channel, an optical detection component, and a carrier disk, wherein the optical detection component provides a detection optical path that penetrates the flow channel, and the optical detection component is used to sense a target biological particle; providing a sample; diluting the sample to form a test solution; controlling the test solution to flow into the flow channel from an inlet end; and when the optical detection component senses the target biological particle in the test solution, the carrier disk is controlled to move to an outlet end of the flow channel, wherein the carrier disk contains a diluent, and a portion of the solution containing the target biological particle in the test solution is controlled to be discharged from the outlet end and then loaded onto the carrier disk to dilute the portion of the solution.

A handling device for biological particles includes a substrate, a metal conductive layer, a working electrode and at least one dielectric layer. The metal conductive layer is on the substrate. The working electrode is arranged on the metal conducting layer and is electrically connected with the metal conducting layer. The at least one dielectric layer covers the metal conducting layer, the at least one dielectric layer is provided with a first groove, the first groove exposes the working electrode, the top surface of the working electrode is higher than the bottom of the first groove, and the at least one dielectric layer contacts the side wall of the working electrode. The first groove can prevent the working electrode from contacting and damaging the target biological particles.