8795results about "Fluorescence/phosphorescence" patented technology

The invention relates to a saline intrusion measurement method, system and equipment based on dynamic ship motion conditions and a medium, and aims to solve the technical problem that the salinity inversion precision is reduced due to spatial-temporal dislocation between particle image velocity measurement flow field data and plane laser-induced fluorescence concentration field data caused by ship six-degree-of-freedom motion. According to the method, future poses are predicted by collecting ship motion parameters and environment disturbance parameters, and space-time compensation parameters are generated in combination with a static calibration matrix; performing three-dimensional coordinate transformation and time synchronization on synchronously acquired original flow field and concentration field data by applying compensation parameters, and eliminating space-time deviation caused by ship movement; a three-dimensional salinity distribution field is calculated based on registration data fusion flow field diffusion characteristics and concentration field distribution; and meanwhile, the pose of the measuring equipment is dynamically adjusted through the closed-loop controller so as to optimize the data acquisition quality. According to the method, ship motion interference is effectively inhibited, and accurate characterization of saline invasion frontal surface spatial characteristics is guaranteed.

A sample holder includes a first member featuring a first retaining mechanism configured to retain a first substrate that includes a sample, a second member featuring a second retaining mechanism configured to retain a second substrate that includes a reagent medium, and an alignment mechanism connected to at least one of the first and second members, and configured to align the first and second members such that the sample contacts at least a portion of the reagent medium when the first and second members are aligned.

The invention belongs to the technical field of river and lake water quality monitoring, and discloses a river and lake water quality intelligent traceability monitoring system and method.Dissolved organic matter fluorescent fingerprints are analyzed in real time through a three-dimensional fluorescent monitoring unit, pollution markers are detected, and parameters such as COD, ammonia nitrogen and total phosphorus are synchronously detected through a full-spectrum monitoring unit; the video monitoring unit is used for collecting section images / videos of rivers and lakes, other sensors are used for collecting real-time values of chlorophyll, pH, conductivity, turbidity and dissolved oxygen in a water body, then the data processing module is used for carrying out normalization processing on multi-source data, and the analysis and early warning module is used for analyzing water quality early warning scores, spectrum early warning scores and video early warning scores. All scores are synthesized to obtain a comprehensive early warning score, traceability is started when comprehensive early warning is larger than or equal to the grade II, pollution sources are recognized based on three-dimensional fluorescence spectrum comparison and upstream patrol, and a river and lake water body monitoring-early warning-traceability-verification closed loop is achieved.

The invention particularly relates to a water pollution intelligent traceability system based on a three-dimensional fluorescence spectrum, which relates to the technical field of environmental monitoring, and comprises a water sample collection and three-dimensional fluorescence data acquisition module, a data preprocessing and PARAFAC component decomposition module, a multi-source pollution characteristic fingerprint database module and an intelligent comparison and pollution source analysis module. According to the method, standard acquisition and preprocessing are adopted, and the original data quality is ensured by means of scattering rejection, instrument correction and the like; pure fluorescence components are decomposed through a PARAFAC algorithm, and feature extraction reliability is guaranteed through triple verification. In the comparison link, spectrum similarity, concentration proportion difference and space-time hydrological constraint multi-round screening are adopted, and irrelevant pollution sources are removed; and then the contribution proportion is quantified, and Monte Carlo simulation, water quality and specific pollutant cross validation are carried out, so that the crossing from suspected tracing to quantitative responsibility investigation is realized.

A wide-field dynamic magnetic imaging device and method. The wide-field dynamic magnetic imaging device comprises: a probe assembly (1), comprising a diamond (101) containing NV centers; a microwave magnetic field assembly (2) configured to generate a uniform microwave field and a uniform bias magnetic field in the vicinity of the diamond (101), so as to regulate the spin energy level of the diamond (101) and the quantum state evolution process; a laser modulation assembly (3) configured to output an initial uniform pulsed laser to excite the diamond (101) to emit fluorescence; a fringe light modulation assembly (4) configured to modulate the initial uniform pulsed laser to output a fringe pulsed laser, causing the fluorescence emitted by the diamond (101) to be fringe fluorescence, or to modulate the fluorescence emitted by the diamond (101) into fringe fluorescence; a fluorescence detection assembly (5) configured to perform spatial imaging on the fringe fluorescence to obtain magnetic field information of a sample under test (7), thereby obtaining an initial magnetic image of said sample (7); and a data processing assembly (6) configured to perform Fourier transform and inverse Fourier transform on the initial magnetic image to obtain a first magnetic image.

The invention discloses a transformer oil leakage detection method and device based on polarization-ultraviolet fusion imaging, and the device comprises a housing, a support, a camera, an environment induction device, an overall controller, a power supply, a polaroid rotating wheel assembly, a lens seat assembly, and an image processor. And the motor driver, the illumination intensity sensor, the ultraviolet irradiance sensor, the image processor and the power supply are electrically connected with the integral controller respectively. According to the invention, a multi-angle polarization image and a non-polarization image in the same field of view can be acquired, and then polarization characteristics contained in an oil stain fluorescence signal are extracted and effectively distinguished from non-polarization characteristics of background light; in the daytime mode, the transformer oil leakage detection device can identify a target area with relatively high polarization intensity, and through image fusion enhancement processing, non-target background signals are significantly suppressed, and the fluorescence significance of an oil leakage characteristic area is improved.

The invention relates to a method for measuring optical lenses (10) using a device (1) comprising: a detection device (11) arranged on a first side (10a) of the lens (10) with respect to a plane of extension of the lens (10); one or more first radiation sources (12) with which the position of the lens (10) in space is determined; and / or a second radiation source (13) with which a planar refractive power distribution of the lens (10) over the surface is determined. According to the invention, a planar, moving pattern extending through the lens (10) is generated by the second or a further radiation source (13), which is detected by the detection device (11) through the lens (10), and one or more defects (15) on the surface of the lens (10) are detected by the computer unit (14).

Systems and methods are provided for targeting one or more features in a region of interest, and efficiently removing the features from an array of features. The systems and methods can remove one or more beads that contain analytes, intermediate agents, or a combination thereof, from the region of interest of a substrate by emitting light toward the beads on the substrate.

Methods and devices for analyzing a sample from a subject are provided. A device includes one or more coherent light sources and one or more partially coherent light sources. The light sources are configured to emit first and second wavelengths of electromagnetic radiation towards an imaging chamber configured to hold blood cells. The light sources are aligned to illuminate a single common area of the imaging chamber. The device includes optics positioned to receive light from the imaging chamber, and a detector in optical communication with the optics. The detector is configured to detect fluorescence emission and backscatter from blood cells when present in the imaging chamber. The device includes mixers / unmixers configured to replicate and mix first and second signals representative of the responses to the first and second wavelengths after interacting with the plurality of blood cells to generate spectro-spatial responses.

The utility model relates to the field of microfluidic detection and fluorescence immunoassay, in particular to a portable biochemical blood coagulation and fluorescence immunoassay integrated analysis device, which comprises a shell, a power supply arranged in the shell, two support plates arranged in the shell, a fluorescent bracket fixedly connected to the upper ends of the support plates, a support arranged in the shell, a lamp source arranged at the upper end of the support, and a light source arranged at the lower end of the lamp source. A microfluidic detection module is mounted in the support, a fluorescence immunodetection module is mounted at the upper end of the fluorescence bracket, an optical module is arranged in the fluorescence immunodetection module, and a bar code scanner for confirming information of a detection chip or a reagent strip is arranged on one side of the optical module; compared with the traditional detection equipment with a single function, the double detection functions of biochemistry and fluorescence immunity are realized, the detection efficiency and comprehensiveness are greatly improved, meanwhile, the kit also supports the simultaneous detection of three reagent strips with different specifications, and the flexibility and convenience of the detection are further enhanced.

This disclosure provides methods for spatial profiling of biological analytes present in a biological sample. Methods include generating feature arrays using a master / copy format using recessed arrays, and methods for using such arrays. For example spatially-tagged analyte capture analytes can be used in spatial detection in methods to determine the location of analytes (e.g., proteins) in biological samples.

The present invention discloses a three-dimensional fluorescence portable water quality analyzer for pollution component determination and source tracing, and relates to the technical field of water quality analysis, the analyzer comprises the following components: a sample collection and processing module for collecting a water sample from a to-be-detected water body, filtering to remove impurities, and diluting the water sample according to a self-adaptive dilution multiple algorithm; fluorescence intensity information of a water sample under different excitation wavelengths and emission wavelengths is obtained through the three-dimensional fluorescence detection module, rich three-dimensional fluorescence spectrum data is formed, the real-time fluorescence lifetime imaging module is introduced, and by measuring the fluorescence lifetime of a fluorescent substance and utilizing the principle that different fluorescent substances have different fluorescence lifetime characteristics, the real-time fluorescence lifetime of the water sample is obtained. Various pollution components in the water sample are further distinguished and recognized, the pollution components can be accurately distinguished through fluorescence lifetime measurement and imaging, the accuracy of pollution component judgment is greatly improved, and a reliable basis is provided for accurate treatment of water quality pollution.

A sample holder includes a first member featuring a first retaining mechanism configured to retain a first substrate that includes a sample, a second member featuring a second retaining mechanism configured to retain a second substrate that includes a reagent medium, and an alignment mechanism connected to at least one of the first and second members, and configured to align the first and second members such that the sample contacts at least a portion of the reagent medium when the first and second members are aligned.

The invention discloses a complex water body pollution detection method and system based on an adaptive cruise technology, and belongs to the technical field of environment monitoring. The method overcomes the inherent defect that an existing monitoring scheme based on a static preset path cannot adapt to a dynamic pollution environment by constructing a closed-loop feedback control architecture of real-time sensing, central decision making and dynamic execution. Generating initial global pollution distribution estimation and planning an initial path by the main aircraft; the subordinate vehicle sails along the path and estimates the local pollution concentration and the gradient direction in real time; and the main aircraft fuses all data to update global estimation, and dynamically generates an instruction for guiding the subordinate aircraft to move towards a pollution concentration gradient rising direction after identifying a pollution plume or a hot spot, thereby realizing active tracking and high-resolution surveying and mapping. According to the invention, the efficiency, precision and intelligent response capability of pollution monitoring are significantly improved, and accurate and adaptive tracking of a dynamic pollution field is realized.

The invention discloses a water quality safety monitoring and early warning method based on big data, and relates to the technical field of monitoring and early warning. Setting sampling points according to areas, pasting scene labels, and arranging sensors to obtain water quality information; establishing a water quality evaluation model based on a support vector machine improved model, inputting the water quality information into the water quality evaluation model, outputting to obtain a water quality category, and dividing the sampling points into normal sampling points and key sampling points according to the water quality category; based on the normal point data, using LSTM to predict water quality safety and performing graded early warning; based on the key point data, positioning a pollution source by using space-time Kriging interpolation, and simulating a pollution diffusion path by using multivariable collaborative interpolation; and combining normal point early warning and key point diffusion simulation to obtain a water quality safety monitoring early warning result. According to the method, a global risk grading report is generated by fusing a normal sampling point early warning result and a key sampling point diffusion path, and emergency response and long-term treatment strategies are matched.

The invention relates to the technical field of electronics, and discloses a drainage pipe network pollutant tracing method, device and equipment, a medium and a program product. According to the method, the map similarity between a first three-dimensional fluorescence spectrum map corresponding to a first water sample of a target node and a second three-dimensional fluorescence spectrum map corresponding to each upstream pipe network node is calculated; and determining at least one target upstream pipe network node in the plurality of upstream pipe network nodes based on the map similarity, and determining pollution source information of abnormal water in a target node according to first water quality data of the target upstream pipe network node and second water quality data on a sewage flowing path between the target upstream pipe network node and the target node, the advantages of high sensitivity, rapid detection and the like of the three-dimensional fluorescence spectrum technology are utilized in the pollutant traceability process, and efficient and accurate traceability of pipe network pollutants is achieved.

The invention discloses a water sample pollution traceability analysis method fusing absorption-three-dimensional fluorescence spectrum characteristics, and relates to the field of water sample spectrum analysis traceability. According to the method, high-precision prediction of pollutant indexes is realized by utilizing multi-dimensional information of a three-dimensional fluorescence spectrum and an absorption spectrum of a water sample, qualitative and quantitative traceability of main pollution components and potential emission sources in a water body is carried out based on correlation analysis of the prediction indexes, a pollutant feature library and an emission source database, and the method has the advantages of high accuracy and high accuracy. The integration of water quality monitoring and pollution source management is realized, and a systematic and real-time technical means is provided for pollution analysis of a complex water body.

The invention discloses an all-optical three-dimensional scanning confocal fluorescent microscopic imaging device and an implementation method thereof. According to the invention, through a deep learning driven adaptive regulation and control method, CNN is adopted to process spatial distribution data and dynamically regulate and control a phase hologram and the light intensity and phase compensation of a laser, so that the focal point of exciting light is subjected to aberration-free axial displacement, a bidirectional parallel optical scanning track of a two-dimensional scanning system is optimized, and all-optical three-dimensional scanning is realized; lSTM and TCN are combined to obtain a time sequence dependency relationship, a laser light source, an adjustable diaphragm, an electric focus-adjustable lens and a photoelectric detector are integrally controlled, efficient synchronization and automatic operation and high-speed axial focusing adjustment are realized, errors and time sequence mismatch are eliminated, optical characteristics of different samples and environmental interference are automatically adapted, and high-quality imaging is kept. The robustness and the applicable scene range of the system are improved; the method is used for model biological embryo real-time tracking, intracellular signal molecule dynamic visualization, cell membrane protein migration and aggregation observation and intracellular organelle interaction tracking.

The invention discloses an indole-benzothiadiazole derivative as well as a preparation method and application thereof. The general structural formula of the indole-benzothiadiazole derivative provided by the invention is shown in the specification, wherein R is selected from one of methyl piperazinyl, diethylamino, azetidinyl and pyrrolidinyl. The derivative can be specifically combined and fluorescently labeled with mitochondrial RNA in cells, and has good selectivity, high fluorescence signal-to-noise ratio and excellent anti-interference capability in a complex biological system. Meanwhile, the indole-benzothiadiazole derivative provided by the invention is simple in synthetic route, easily available in raw materials, good in stability and convenient to store and use, can be specifically combined with RNA and generate strong fluorescence response, is suitable for real-time detection and imaging of in-vitro and in-vivo mitochondrial RNA, and is an efficient mitochondrial RNA micromolecular fluorescent probe.

The invention discloses a multi-mode nonlinear optical microscopic imaging device and system, and the device achieves the two-photon and three-photon excitation imaging of a sample through an integrated light source module, a dispersion compensation module, a scanning light path module, a fluorescence obtaining module, a synchronous control module and a signal processing module. Fluorescence intensity imaging and fluorescence lifetime imaging can be supported at the same time; wherein the scanning light path module is driven by the random mask to realize sparse scanning, so that scanning point locations are effectively reduced, and the exciting light dosage is reduced; the synchronous control module performs time window screening and processing on the fluorescence signal based on the pulse synchronous signal and the position code, so that the signal acquisition efficiency is improved; the signal processing module adopts an FPGA (Field Programmable Gate Array) to carry out real-time image reconstruction and life calculation, so that the imaging speed and quality are ensured; and the master control PC can also perform high-fidelity reconstruction on the sparse sampling image through a deep learning model, so that high-speed, low-light-dose and high-resolution nonlinear fluorescence intensity and life imaging is realized.

The invention relates to the field of environmental monitoring, and particularly discloses a non-negative matrix factorization and adaptive peak recognition fluorescence feature extraction and traceability system, which comprises a spectral data preprocessing module, a spectral data non-negative matrix factorization module, a component number automatic selection module, an adaptive peak recognition module, a feature library construction module and a similarity comparison module. An improved non-negative matrix factorization model is adopted to decompose the three-dimensional fluorescence spectrum matrix of a single sample, and an optimal component number K is automatically determined through multiplicative update rule iterative optimization; the self-adaptive peak identification module carries out selective filtering, accurately extracts the position and intensity of a fluorescence peak through multiple mechanisms, and carries out peak position calibration in a neighborhood; the Hungary algorithm is adopted to carry out characteristic peak matching to calculate the comprehensive similarity between the samples, and rapid and accurate identification of the pollution source is realized. The method has the advantages of high resolution, strong anti-interference capability, low requirement on the number of samples, automation and the like, and is suitable for water quality fingerprint feature extraction of a water sample in a complex environment and real-time source tracing of sewage.

The invention relates to the technical field of oil stain detection, and discloses an oil-filled equipment oil stain detection method based on fluorescence reflection. The method comprises the following steps: carrying out cleaning pretreatment on a to-be-detected surface; an ultraviolet light source is utilized to excite the oil stain to generate fluorescence; collecting a fluorescence image through an imaging device with a filter; performing noise reduction and edge processing on the image to extract an oil stain area; and finally judging the existence and type of oil stain and estimating the thickness according to image features. According to the invention, the fluorescence characteristic of the oil stain is utilized, no fluorescent agent needs to be added, and pollution-free, high-precision and high-efficiency non-contact detection is realized.

The invention discloses a sugar chain marker combination for predicting prognosis of liver cancer transarterial chemoembolization and application of the sugar chain marker combination. The invention discovers and verifies that the combination composed of NA3Fb, NA4Fb and NA4F2b can be used as a novel marker for evaluating TACE treatment reaction and prognosis by analyzing a serum sugar chain map of a liver cancer patient for the first time. Based on the marker combination, a TACE-GT prognosis scoring model and system are established, and the progression risk of a patient within 12 months after a TACE operation can be effectively distinguished by noninvasively detecting the expression levels of three carbohydrate chains in serum of the patient and calculating a specific score. The detection method provided by the invention is based on a blood sample, and has the advantages of noninvasiveness and convenience, and a risk assessment system can provide individualized curative effect prediction and decision support for clinicians before and after TACE treatment, and has important values for realizing precise treatment of liver cancer and improving prognosis of patients.

The invention discloses a dual-mechanism antibacterial photosensitizer as well as a preparation method and application thereof, and relates to the technical field of biomedical photoelectric functional materials. The invention relates to a triphenylamine derivative, which can realize fluorescence imaging of bacterial or fungal infection by utilizing fluorescence characteristics, can generate active oxygen under illumination to play a photodynamic therapy role, is suitable for preparing photodynamic therapy preparations and developing antibacterial materials, and can also be applied to the fields of environment and public health and tumor treatment. The problems that an existing photosensitizer is insufficient in broad spectrum, different in fungus drug resistance mechanism, limited in single treatment effect and the like can be effectively solved, and the photosensitizer has wide application prospects.

The invention discloses a correction method for saturation spectral line intensity in a laser spectrum, and relates to the technical field of spectral data analysis, the method comprises the following steps: identifying saturation characteristics in the spectrum, determining a saturation point based on an intensity threshold, and recording the original intensity of the saturation point; determining a target spectrum peak wavelength range containing a saturation point; eliminating saturation points from the wavelength range of the target spectrum peak, and retaining unsaturation points; fitting the unsaturated point through a nonlinear function to obtain the maximum fitting strength; if the maximum fitting strength is lower than the original strength, performing strength correction by adopting a linear extension mode by taking an unsaturated point as a reference, and otherwise, performing strength correction directly based on a fitting model; and outputting the corrected spectral intensity data, and calculating the maximum intensity of the spectral peak and the integral area of the spectral peak. According to the method, the accuracy of saturation judgment and the reliability of a correction result are improved, and a direct intensity data basis and core derivative parameters are provided for quantitative analysis.

Methods for determining a location of a feature in a spatial array with features include: (a) providing an array with a first set of one or more features immobilized on a substrate, a first feature of the first set having a first barcoded oligonucleotide with a first spatial barcode and a first constant sequence, and a second set of one or more features immobilized on the substrate, a second feature of the second set having a second barcoded oligonucleotide with a second spatial barcode and a second constant sequence; (b) attaching the first constant sequence to the second constant sequence to generate a nucleic acid product; (c) determining all or a portion of a sequence of the nucleic acid product or a complement thereof; and (d) associating the second barcoded oligonucleotide with the first barcoded oligonucleotide in the nucleic acid product.

The invention provides an underground water environment monitoring system and method, and the method comprises the steps: carrying out the three-dimensional fluorescence spectrum full-wave band scanning of a known type of pollution source reference sample in a target monitoring region, and obtaining a three-dimensional fluorescence spectrum data set; performing characteristic analysis on the three-dimensional fluorescence spectrum data set to obtain fluorescence characteristic components, and determining a characteristic excitation wavelength corresponding to each fluorescence characteristic component; the classification contribution degree of each characteristic excitation wavelength is evaluated, and a characteristic excitation wavelength combination during source tracing of the pollution source is obtained through screening; performing fixed wavelength scanning on the to-be-detected underground water sample in the target monitoring area according to the characteristic excitation wavelength combination, and determining the initial type of the pollution source; and determining the relative contribution degree of the type of the pollution source of the to-be-detected underground water sample, simulating the migration trend of the pollution plume according to the underground water migration and diffusion model, and generating the position of the pollution source of the to-be-detected underground water sample. By adopting the scheme of the application, the pollution source of the underground water can be quickly identified and positioned at low cost.

The invention discloses a method for detecting the concentration of cyclosporine in blood through fluorescence and electrochemical double signals, and belongs to the technical field of biochemical analysis and medical detection. Aiming at the technical problems of insufficient sensitivity and high false positive rate of traditional single-signal detection of blood concentration of cyclosporine, the method comprises the following steps: activating carboxyl microspheres by a coupling agent, fixing double-stranded DNA (deoxyribonucleic acid) formed by a cyclosporine aptamer and an initiator chain, inducing the initiator to release by using a cyclosporine sample, realizing strand displacement through an A2-PEI composite system, and detecting the blood concentration of cyclosporine by using an A < 2 >-PEI composite system. DNA modified cadmium telluride quantum dots are respectively adopted to detect fluorescence signals, eATRP electrochemical signal amplification is carried out to detect electrochemical signals, concentration values obtained by the two signals are verified, and an average value is taken as a result. The method can accurately capture low-concentration cyclosporine signals in blood, eliminates interference of a single method, is mainly used for accurately monitoring the blood concentration of cyclosporine, and provides a reliable detection means for medication safety and curative effect control of cyclosporine in clinical scenes such as organ transplantation.

The invention provides a three-order angular distance extraction-based emulsified asphalt polymer distribution uniformity evaluation method, which comprises the following steps of: acquiring a fluorescent microphotograph of an emulsified asphalt polymer modified sample, carrying out RGB three-channel separation, constructing a joint probability matrix with a gray vector on polar coordinates, and simultaneously carrying out normalization processing; and further calculating a first-order angular moment, a second-order angular moment and a third-order angular moment of the fluorescent microscopic image of the emulsified asphalt polymer modified sample according to the joint probability matrix of the gray vector, and evaluating the distribution uniformity of the polymer in the emulsified asphalt matrix. The method can accurately quantify the distribution uniformity of the polymer in the emulsified asphalt, is small in test sampling amount and high in test speed, can be widely applied to quality detection of the emulsified asphalt in road engineering construction and maintenance, and provides an effective technical means for improving the modification effect of the polymer of the emulsified asphalt and guiding optimization of a preparation process of the emulsified asphalt.