12912results about "Fluorescence/phosphorescence" patented technology

A sample holder includes a first member featuring a first retaining mechanism configured to retain a first substrate that includes a sample, a second member featuring a second retaining mechanism configured to retain a second substrate that includes a reagent medium, and an alignment mechanism connected to at least one of the first and second members, and configured to align the first and second members such that the sample contacts at least a portion of the reagent medium when the first and second members are aligned.

The invention provides an online quality detection method of a nerve-soothing and brain-nourishing liquid based on multispectral fusion, and aims at solving the bottleneck problems of long offline time consumption, weak multi-index detection capability, poor dynamic adaptability and the like in the traditional detection technology, and realizing intelligent quality control of the whole process of the production technology. The device integrates an ultraviolet-visible spectrum module, a near-infrared spectrum module and a fluorescence spectrum module, constructs a multi-scale residual convolutional network to extract near-infrared spectrum characteristics, analyzes a fluorescence excitation-emission matrix through bidirectional LSTM, and cooperates with dynamic baseline correction of an ultraviolet spectrum, so that the icariin content detection precision is improved by 40% compared with that of a traditional HPLC method, the heavy metal residue detection lower limit reaches 0.05 ppm, and the detection accuracy is greatly improved. And an innovative solution is provided for intelligent production of traditional Chinese medicine preparations.

This disclosure provides methods for spatial profiling of biological analytes present in a biological sample. Methods include generating feature arrays using a master / copy format using recessed arrays, and methods for using such arrays. For example spatially-tagged analyte capture analytes can be used in spatial detection in methods to determine the location of analytes (e.g., proteins) in biological samples.

This disclosure relates to compositions and methods for analyzing a tissue section from a biological sample.

The invention provides a river pollutant traceability method and a river pollutant traceability system. The method comprises the following steps: constructing a potential pollution source feature fingerprint database fused with an LSTM time sequence feature extraction model, carrying out abnormal water quality fingerprint identification on a monitored river reach, if identification is abnormal, collecting an upstream water sample, detecting to obtain a water quality fingerprint, comparing the water quality fingerprint with the abnormal water quality fingerprint, determining a target river reach according to a comparison result, and determining the target river reach according to the comparison result. The method comprises the following steps: sampling all enterprises of a target river reach in real time, comparing detection result data with detection result data of an abnormal water sample, determining potential pollution sources according to a comparison result, determining high-matching candidate sources from the potential pollution sources by utilizing an LSTM (Long Short Term Memory) time sequence feature extraction model, carrying out space transition verification on the high-matching candidate sources, and carrying out space transition verification on the high-matching candidate sources. And determining the pollution source according to the verification result. According to the method, the accuracy, efficiency and result reliability of tracing the river pollutants can be effectively improved, and the scenes of multi-source pollution, intermittent emission and the like of complex rivers can be effectively handled.

Systems and methods are provided for targeting one or more features in a region of interest, and efficiently removing the features from an array of features. The systems and methods can remove one or more beads that contain analytes, intermediate agents, or a combination thereof, from the region of interest of a substrate by emitting light toward the beads on the substrate.

A sample holder includes a first member featuring a first retaining mechanism configured to retain a first substrate that includes a sample, a second member featuring a second retaining mechanism configured to retain a second substrate that includes a reagent medium, and an alignment mechanism connected to at least one of the first and second members, and configured to align the first and second members such that the sample contacts at least a portion of the reagent medium when the first and second members are aligned.

The invention provides a farmland disease and pest monitoring method and device and a storage medium, and relates to the technical field of agriculture, the method comprises the steps: dividing a farmland into a plurality of sub-regions, obtaining an infrared spectrum image, and segmenting the infrared spectrum image into corresponding sub-images; and calculating a vegetation stress index based on the reflectivity of the insect pest related characteristic wave band, and screening high-risk sub-regions. Risk plants are randomly extracted in a high-risk area, physiological parameters of leaves are collected, physiological health indexes of the leaves are constructed, and dynamic correction is carried out through an environment regulation coefficient according to environment data. Using the trained network model to identify the risk sample leaf scab, and calculating the scab area index. And finally, fusing the corrected leaf physiological health index and the scab area index, calculating a normalized risk value through a risk assessment function, and dividing the normalized risk value into four risk levels. Through multi-stage fusion of spectral analysis, physiological parameter correction and deep learning scab detection, accurate early warning of diseases and insect pests is realized, and the monitoring efficiency is improved.

The invention belongs to the technical field of environmental water quality pollution monitoring, and particularly relates to a drainage basin water environment monitoring and emergency pollution rapid tracing method and system based on water quality pollution detection, and the method comprises the following steps: constructing a multi-parameter cooperative monitoring model, and carrying out the training and deployment; through distributed sampling points and sampling stations, real-time data with verification marks and time-sharing data of watershed water environment water quality pollution detection are collected; mLP and LSTM / GRU models are adopted, EEM spectrum data and environment characteristics are fused, and multi-source heterogeneous data are fused and analyzed; whether an emergency pollution event occurs or not is automatically identified according to a preset condition, the pollution types and traceability results of water environment monitoring and emergency pollution are automatically output, manual further checking is carried out, and environmental law enforcement checking is carried out. According to the invention, drainage basin water environment pollution condition monitoring and emergency pollution rapid tracking and tracing can be completed in a large-range, low-cost and high-efficiency manner so as to support environment law enforcement.

The invention relates to a saline intrusion measurement method, system and equipment based on dynamic ship motion conditions and a medium, and aims to solve the technical problem that the salinity inversion precision is reduced due to spatial-temporal dislocation between particle image velocity measurement flow field data and plane laser-induced fluorescence concentration field data caused by ship six-degree-of-freedom motion. According to the method, future poses are predicted by collecting ship motion parameters and environment disturbance parameters, and space-time compensation parameters are generated in combination with a static calibration matrix; performing three-dimensional coordinate transformation and time synchronization on synchronously acquired original flow field and concentration field data by applying compensation parameters, and eliminating space-time deviation caused by ship movement; a three-dimensional salinity distribution field is calculated based on registration data fusion flow field diffusion characteristics and concentration field distribution; and meanwhile, the pose of the measuring equipment is dynamically adjusted through the closed-loop controller so as to optimize the data acquisition quality. According to the method, ship motion interference is effectively inhibited, and accurate characterization of saline invasion frontal surface spatial characteristics is guaranteed.

The invention provides an underground water pollution traceability method and system based on deep learning and multi-source data fusion, and the method employs multi-source data fusion to accurately describe pollution source features, builds a pollution source multi-source data set, builds a pollution source recognition model based on a CNN convolutional neural network and an LSTM long-short-term memory network, and achieves the traceability of underground water pollution. The multi-source data set is used for training, and the features and potential relationships of the multi-source data are fully mined, so that the traceability model can accurately identify the features of the main pollution source and contributions thereof, the limitation of single-dimensional data traceability and the difficulty of multi-source data fusion are overcome, and the accuracy and efficiency of groundwater pollution traceability are improved.

A sample holder includes a first member featuring a first retaining mechanism configured to retain a first substrate that includes a sample, a second member featuring a second retaining mechanism configured to retain a second substrate that includes a reagent medium, and an alignment mechanism connected to at least one of the first and second members, and configured to align the first and second members such that the sample contacts at least a portion of the reagent medium when the first and second members are aligned.

The invention belongs to the technical field of river and lake water quality monitoring, and discloses a river and lake water quality intelligent traceability monitoring system and method.Dissolved organic matter fluorescent fingerprints are analyzed in real time through a three-dimensional fluorescent monitoring unit, pollution markers are detected, and parameters such as COD, ammonia nitrogen and total phosphorus are synchronously detected through a full-spectrum monitoring unit; the video monitoring unit is used for collecting section images / videos of rivers and lakes, other sensors are used for collecting real-time values of chlorophyll, pH, conductivity, turbidity and dissolved oxygen in a water body, then the data processing module is used for carrying out normalization processing on multi-source data, and the analysis and early warning module is used for analyzing water quality early warning scores, spectrum early warning scores and video early warning scores. All scores are synthesized to obtain a comprehensive early warning score, traceability is started when comprehensive early warning is larger than or equal to the grade II, pollution sources are recognized based on three-dimensional fluorescence spectrum comparison and upstream patrol, and a river and lake water body monitoring-early warning-traceability-verification closed loop is achieved.

The invention particularly relates to a water pollution intelligent traceability system based on a three-dimensional fluorescence spectrum, which relates to the technical field of environmental monitoring, and comprises a water sample collection and three-dimensional fluorescence data acquisition module, a data preprocessing and PARAFAC component decomposition module, a multi-source pollution characteristic fingerprint database module and an intelligent comparison and pollution source analysis module. According to the method, standard acquisition and preprocessing are adopted, and the original data quality is ensured by means of scattering rejection, instrument correction and the like; pure fluorescence components are decomposed through a PARAFAC algorithm, and feature extraction reliability is guaranteed through triple verification. In the comparison link, spectrum similarity, concentration proportion difference and space-time hydrological constraint multi-round screening are adopted, and irrelevant pollution sources are removed; and then the contribution proportion is quantified, and Monte Carlo simulation, water quality and specific pollutant cross validation are carried out, so that the crossing from suspected tracing to quantitative responsibility investigation is realized.

The invention relates to microwave-free sensor technology with NV centers in diamond. In contrast to the prior art, the intensity of the pump radiation is not PWM-modulated, but monofrequency and sinusoidally modulated. Further measures, such as the use of semiconductor-based pump radiation sources and operation with an offset, prevent the formation of harmonics in the temporal intensity profile of the pump radiation irradiating the NV centers in the sensor element. This allows the sensor system to use the phase shift to determine measured values ​​of external physical parameters such as magnetic flux density, acceleration, temperature, etc., within a recognized and disclosed frequency range of the intensity modulation of the pump radiation, thereby minimizing the influence of noise from the pump radiation source. The galvanic isolation does not need to be compromised by cables from additional sensors.

The invention relates to the technical field of cytology, in particular to a grass carp brain astrocyte line and application thereof, the grass carp brain astrocyte line is preserved in China Center for Type Culture Collection on May 7, 2025, and the preservation number of the grass carp brain astrocyte line is CCTCC NO: C2025149. The grass carp brain astroglia cell line provided by the invention has the capability of efficiently proliferating GCRV-II, and the virus titer of the GCRV-II replicated in the cell line at least can reach 1.38 * 10 < 8 > pfu / mL or above; after the GCRV-II is blindly passed for 5 generations in a grass carp astroglia cell line, the grass carp can still have typical bleeding symptoms and death due to the virus. And the exogenous plasmid transfected grass carp brain astroglia cell line has similar transfection efficiency to commercial grass carp kidney cells. Therefore, the invention lays an important foundation for deep research of pathogenic mechanism of GCRV-II, vaccine preparation, antiviral drug screening and prevention and control of grass carp viral hemorrhagic disease.

There is proposed a method for detecting a presence of a fluorescence pattern type on an organ segment via immunofluorescence microscopy and digital image processing. The steps comprise: provision of the organ segment, incubation of the organ segment with a liquid patient sample, incubation of the organ segment with secondary antibodies which have been labelled with a fluorescent dye, acquisition of a fluorescence image of the organ segment in a colour channel corresponding to the fluorescent dye, and provision of the fluorescence image to a neural network. What takes place by means of the neural network is simultaneous determination of segmentation information through segmentation of the fluorescence image and, furthermore, of a measure of confidence indicating an actual presence of the fluorescence pattern type. What further takes place is determination, on the basis of the previously determined segmentation information, of at least one sub-area of the fluorescence image that is relevant to formation of the fluorescence pattern type, determination, on the basis of the previously determined at least one sub-area, of validity information indicating a degree of a validity of the measure of confidence, and output of the measure of confidence depending on the validity information.

The invention relates to the technical field of underground engineering groove wall stability physical simulation, and discloses a model test device and method for stratum excavation and grouting reinforcement, and the model test device comprises a grooving construction module, a grouting support module and a dynamic monitoring module. The device adopts a pressing plate method to simulate a grooving excavation process, so that the device conforms to an actual excavation working condition from top to bottom; a servo motor drives a power lead screw to drive a model groove to move downwards, so that a free face is formed in a sand layer, and the actual excavation working condition is simulated; a water tank for storing fresh slurry is pressurized by using an air compressor, so that the slurry overflows from the hollow model groove section, and the permeation and invasion process of the slurry is simulated; and a water and soil pressure gauge and a matched acquisition device are arranged in the sand layer, and a soil displacement field is obtained by utilizing a particle image velocity measurement system, so that the visualization of the whole process of excavation-grouting-invasion is realized. It is ensured that simulation results are highly matched with actual working conditions, and accuracy and reliability are improved.

The invention discloses a high-sensitivity miniaturized HOx free radical accurate measurement device and method, and relates to the technical field of atmospheric environment detection and automatic control, and the device is composed of a free radical generation and removal device, a light source module, longitudinal HOx double cavities, a gas distribution module and a collection and control system. The free radical generation and removal device can generate HOx free radicals or remove OH free radicals; the light source module can emit laser which enables OH free radicals to generate fluorescence and transmit the laser into the longitudinal HOx double-cavity; the longitudinal HOx double cavities are used for exciting OH free radicals to generate a large amount of fluorescence and collecting and converting the fluorescence into electric signals; the gas distribution module is used for injecting gas in the device and maintaining the flowing state of gas flow; and the control and acquisition system is used for controlling automatic operation and data acquisition and processing of the device. The device has the advantages of being high in detection sensitivity, small in size and capable of rapidly obtaining and removing OH free radical measurement interference, rapid calibration of similar concentration can be conducted on the actual HOx concentration in the actual measurement environment, accurate spectrum determination can be conducted on the laser wavelength, and therefore high-sensitivity accurate measurement of HOx free radicals is achieved.

The invention relates to the field of ulcerative colitis, and discloses a multi-parameter monitoring system for evaluating the intestinal barrier function of ulcerative colitis, which is used for realizing the goal of automatically adjusting a treatment scheme according to a monitoring result by constructing a closed-loop feedback treatment system. According to the invention, by designing the nanoprobe of which the surface is modified with a specific binding element and combining with a swallowing capsule endoscopy, real-time imaging and molecular level dynamic tracking of the intestinal barrier are realized, genetically engineered bacteria are constructed to quantify intestinal permeability abnormality and local inflammation level, and an intestinal barrier function damage score and a disease progress prediction result are output; individual monitoring is achieved through transfer learning, ultrasonic elastography, optical coherence tomography, wearable equipment for measuring intestinal electric field impedance spectroscopy and other technologies are adopted, an intestinal wall mechanics-electrophysiology composite parameter set is obtained, and the evaluation accuracy is improved.

The invention discloses a regional GPP assimilation inversion system fusing foundation-multi-source satellite chlorophyll fluorescence, and belongs to the technical field of terrestrial ecosystem carbon cycle monitoring. The signal unmixing and simulation generation module is used for unmixing the total fluorescence of the mixed pixels into pure chlorophyll fluorescence (SIF) and driving an ecological model to generate SIF and GPP simulation values based on environmental stress data; the state component decoupling module is used for decoupling the total GPP analog value into a light response fast component, a light response slow component and a light response memory component; the state evolution prediction module is used for predicting dynamic evolution of the slow component and the memory component; and the self-adaptive assimilation inversion module is used for performing inversion on the region GPP by taking the slow component and the memory component as state variables, taking the SIF observation value as observation data and combining a prediction result of the state evolution prediction module. According to the method, systematic deviation caused by model simplification or data singleness in a traditional method is overcome.

A wide-field dynamic magnetic imaging device and method. The wide-field dynamic magnetic imaging device comprises: a probe assembly (1), comprising a diamond (101) containing NV centers; a microwave magnetic field assembly (2) configured to generate a uniform microwave field and a uniform bias magnetic field in the vicinity of the diamond (101), so as to regulate the spin energy level of the diamond (101) and the quantum state evolution process; a laser modulation assembly (3) configured to output an initial uniform pulsed laser to excite the diamond (101) to emit fluorescence; a fringe light modulation assembly (4) configured to modulate the initial uniform pulsed laser to output a fringe pulsed laser, causing the fluorescence emitted by the diamond (101) to be fringe fluorescence, or to modulate the fluorescence emitted by the diamond (101) into fringe fluorescence; a fluorescence detection assembly (5) configured to perform spatial imaging on the fringe fluorescence to obtain magnetic field information of a sample under test (7), thereby obtaining an initial magnetic image of said sample (7); and a data processing assembly (6) configured to perform Fourier transform and inverse Fourier transform on the initial magnetic image to obtain a first magnetic image.

The invention discloses a transformer oil leakage detection method and device based on polarization-ultraviolet fusion imaging, and the device comprises a housing, a support, a camera, an environment induction device, an overall controller, a power supply, a polaroid rotating wheel assembly, a lens seat assembly, and an image processor. And the motor driver, the illumination intensity sensor, the ultraviolet irradiance sensor, the image processor and the power supply are electrically connected with the integral controller respectively. According to the invention, a multi-angle polarization image and a non-polarization image in the same field of view can be acquired, and then polarization characteristics contained in an oil stain fluorescence signal are extracted and effectively distinguished from non-polarization characteristics of background light; in the daytime mode, the transformer oil leakage detection device can identify a target area with relatively high polarization intensity, and through image fusion enhancement processing, non-target background signals are significantly suppressed, and the fluorescence significance of an oil leakage characteristic area is improved.

The invention relates to a method for measuring optical lenses (10) using a device (1) comprising: a detection device (11) arranged on a first side (10a) of the lens (10) with respect to a plane of extension of the lens (10); one or more first radiation sources (12) with which the position of the lens (10) in space is determined; and / or a second radiation source (13) with which a planar refractive power distribution of the lens (10) over the surface is determined. According to the invention, a planar, moving pattern extending through the lens (10) is generated by the second or a further radiation source (13), which is detected by the detection device (11) through the lens (10), and one or more defects (15) on the surface of the lens (10) are detected by the computer unit (14).

Systems and methods are provided for targeting one or more features in a region of interest, and efficiently removing the features from an array of features. The systems and methods can remove one or more beads that contain analytes, intermediate agents, or a combination thereof, from the region of interest of a substrate by emitting light toward the beads on the substrate.

The invention discloses a sky-ground integrated sunlight-induced vegetation chlorophyll fluorescence monitoring system, and belongs to the technical field of remote sensing monitoring. The system provides functions, parameter constraints and cooperation relations of space-based, empty-based and foundation chlorophyll fluorescence information acquisition and data analysis processing and application modules. Spatial scale matching, spectral scale matching, observation angle matching and atmospheric element matching are performed on the acquired sky-ground multi-element data with different scales, so that reflection characteristics, heat dissipation, photochemical indexes, fluorescence emission and atmospheric transmission characteristics during vegetation photosynthesis can be completely acquired; the invention provides a parameter relation and a method for extracting a fluorescence full-spectrum radiation information curve based on oxygen absorption and Fraunhofer dark line. On the basis of sky and landmark calibration and model verification, chlorophyll fluorescence detection of different fineness degrees of global large-range, key areas and ground crown planting scales can be carried out, various stress effects of vegetation can be monitored in real time, and the primary total productivity of the vegetation can be accurately estimated.

The present disclosure relates to systems and methods for facing a tissue block. In some embodiments, a method is provided for facing a tissue block that includes imaging a tissue block to generate imaging data of the tissue block, the tissue block comprising a tissue sample embedded in an embedding material, estimating, based on the imaging data, a depth profile of the tissue block, wherein the depth profile comprises a thickness of the embedding material to be removed to expose the tissue sample to a pre-determined criteria, and removing the thickness of the embedding material to expose the tissue to the pre-determined criteria.

Methods and devices for analyzing a sample from a subject are provided. A device includes one or more coherent light sources and one or more partially coherent light sources. The light sources are configured to emit first and second wavelengths of electromagnetic radiation towards an imaging chamber configured to hold blood cells. The light sources are aligned to illuminate a single common area of the imaging chamber. The device includes optics positioned to receive light from the imaging chamber, and a detector in optical communication with the optics. The detector is configured to detect fluorescence emission and backscatter from blood cells when present in the imaging chamber. The device includes mixers / unmixers configured to replicate and mix first and second signals representative of the responses to the first and second wavelengths after interacting with the plurality of blood cells to generate spectro-spatial responses.

The utility model relates to the field of microfluidic detection and fluorescence immunoassay, in particular to a portable biochemical blood coagulation and fluorescence immunoassay integrated analysis device, which comprises a shell, a power supply arranged in the shell, two support plates arranged in the shell, a fluorescent bracket fixedly connected to the upper ends of the support plates, a support arranged in the shell, a lamp source arranged at the upper end of the support, and a light source arranged at the lower end of the lamp source. A microfluidic detection module is mounted in the support, a fluorescence immunodetection module is mounted at the upper end of the fluorescence bracket, an optical module is arranged in the fluorescence immunodetection module, and a bar code scanner for confirming information of a detection chip or a reagent strip is arranged on one side of the optical module; compared with the traditional detection equipment with a single function, the double detection functions of biochemistry and fluorescence immunity are realized, the detection efficiency and comprehensiveness are greatly improved, meanwhile, the kit also supports the simultaneous detection of three reagent strips with different specifications, and the flexibility and convenience of the detection are further enhanced.