The invention belongs to the technical field of traditional Chinese medicinal preparation quality control, and concretely discloses a Kangshen granule fingerprint construction method. The method comprises the following steps: preparing a sample solution and a reference substance solution, determining high performance liquid chromatography conditions, and determining fingerprint; and the high performance liquid chromatography conditions are as follows: octadecylsilane chemically bonded silica is adopted as a filler, the detection wavelength is 270-290 nm, the column temperature is 20-40 DEG C, the flow velocity is 0.8-1.2 ml/min, a mobile phase is composed of a mobile phase A and a mobile phase B, acetonitrile is adopted as the mobile phase A, 0.5-1.5% of a formic acid-water solution is adopted as the mobile phase, and linear gradient elution is carried out. The Kangshen granule fingerprint constructed in the invention is formed by chromatographic information of multiple common peaks, a technology for controlling the quality by using a single index component is changed, and the substance of the granule is systemically and comprehensively reflected, so comprehensive and effective quality control is realized, and the established method good precision, repeatability and stability, and can be used for controlling the inner quality of the Kangshen granule.